two genomes

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Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populations as inferred from complete mtDNA genome sequences Steven M Carr Terra Nova Genomics, Inc. & Dept of Biology, Memorial University of Newfoundland St John's NL, Canada 2 nd International Conference on Oceanography Las Vegas NV, 21 July 2014

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Page 1: Two Genomes

Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populations

as inferred from complete mtDNA genome sequences

Steven M CarrTerra Nova Genomics, Inc. &

Dept of Biology, Memorial University of NewfoundlandSt John's NL, Canada

2nd International Conference on OceanographyLas Vegas NV, 21 July 2014

Page 2: Two Genomes

Two Genomes

nucDNA genome2,850,000,000 bp

20,500 genes

mtDNA genome~17,000 bp38 genes

Page 3: Two Genomes

DNA Sequencing on a MicroarrayComplete mtDNA genome in one experiment

[ SM Carr et al. 2008. Comp Biochem Physiol D 3:1-11 ]

Page 4: Two Genomes

Molecular Velcro: Multi-species ArkChip(Three fish, three mammals, one bird: 115,984 bp)

Page 5: Two Genomes

Information content of DNA microarrays:7 species X 80 individuals / spp X 16.5 Kbp @ = 9.2 Mbp

Page 6: Two Genomes

Atlantic Cod(Gadus morhua)

Newfoundland Caribou(Rangifer tarandus)

Harp Seals(Pagophilus groenlandicus)

Atlantic Wolffish(Anarhicas lupus)

Page 7: Two Genomes

Harp Seal pupping & breeding grounds

Gulf

Front

GreenlandSea

WhiteSea

Page 8: Two Genomes

Genomic Phylogeography of Pagophilus16.5 Kbp @ from 83 Harp seals

[ Carr, Duggan, Stenson, & Marshall 2014 PLoS ONE, in review ]

Page 9: Two Genomes

White Sea

Greenland Sea

Ice Front

Gulf

Front ‘99

Stronger geographic structures require fewer migration events;Compare observed tree versus 10,000 random trees

Is the observed tree geographically structured?

Page 10: Two Genomes

Are populations geographically structured ?

Isolation-by-Distance

YES: Trans-Atlantic “Stepping-stone” modelrequires significantly fewer migration events than random expectation

Page 11: Two Genomes

Population expansion wrt Climate Change over 300 Ky

Wisconsinanglaciation

Page 12: Two Genomes

Testing structure of populations within speciesKolmogorov – Smirnov non-parametric test:

Are cumulative genetic distance curves significantly offset?

YES: Diversity among Greenland Sea Harp Seals is youngerthan among White Sea seals [ versus ]

Page 13: Two Genomes

Spawning Stocks of Atlantic CodAre they genetically distinguishable populations

?

Page 14: Two Genomes

Family Tree of Atlantic Cod:Northern Cod (2J3KL) vs Trans-Laurentian stocks (4WX5Ze)

vs Flemish Cap (3M) vs Norwegian Coastal stocks[ Carr & Marshall 2008 Genetics 180,381 & in progress ]

Bay Cod

Page 15: Two Genomes

Worst-Case Scenario:Oil Spill impacts Genome Ecosystem

Page 16: Two Genomes

Genetic Consequences of Environmental Disruption

Loss of connectivity: disruption of gene flow & dispersal

Local success & dispersal

Gene Flow

Page 17: Two Genomes