253 254

UNUSUAL LUNG METASTASIS FROM CERVICAL AND NASOPHARYNGEAL CANCER: IDENTIFICATION BY F’OLYMERASE CHAIN REACMON AND IN SITU HYBRIDIZATION. M.F. Wu, K.C. Chow, W.K. Chan, W.Y. Li, K.Y. Chen, R.P. Perng. Veterans General Hospital-Taipei, Taiwan, R.O.C.

Metastatic lung cancer of uterine cervix or nasopharynx presenting as a central endobronchial tumor obstruction with lobar collapse or as a peripheral mass with medktbml lymphadenopathy is very rare. However, it is important to differentiate between metastatic lung cancer from primary hmg cancer because of difference in treatment plan. We present a case of epidermoid carcinoma of cervix, stage Ib, after radical hysterectomy and adjuvant pelvic radiotherapy and a case of carcinoma of nasopbatynx, stage T4N3M0, after local radiotherapy, both bad lung tumor mimic primary lung cancer years later. It was difficult to distinguish between primary or metastatic lung cancer by light microscopy and other conventional diagnostic methods. using polymerase chain reaction and in situ hybrid&ion techniques, we were able to demonstrate the presence of identical human papillomavirus DNA or Epstein-Barr virus DNA in the nuclei of the neoplastic cells from the primary site and the lung tumor tissues. Metastatic lung cancer was thus confirmed. The detail of the influence on treatment and the results will be discussed.

255

THE VALUE OF LUNG FUNCTION MEASUREMENTS FOR THE ASSESSMENT OF CIKMOTHERAF'Y OF LUNG CANCER PATIENTS. P.Pinson, J.P. Sculier, J.Klastersky. Institut Jules Bordet, Brussels, BelgiUIO..

To evaluate lung function tests for assessing tumor res- ponse of patients with lung cancer to chemotherapy, 95 pa- tients files were retrospectively screened for the availa- bility of data on lung function testing before and after 3 cycles of chemotherapy; 44 files (26 non small cell and 18 small cell lung cancers) contained sufficient data (i.e.X- ray and CT scan of the chest, bronchoscopy, lung function tests before and after 3 courses of chemotherapy). On the basis of radiologic and bronchoscopic findings at presenta- tion, patients were separated in 4 groups : obstructive, restrictive, luated in all

coin lesions and "mixed" forms. FEV1 was eva- groups, while Raw was evaluated in the ob-

structive and mixed groups and TLC, DLCO in the restrictive and mixed groups.

before chemotherapy after 3 cycles FEVl resp.* 1,74 1.97 p=O.O58 (l/s) non-resp.** 1,50 1.49 p=O.48 Raw resp. 2,47 2.08 p=o.4 (cmli2O/l.s) non-resp. insufficient data TLC resp. 4,69 5.2 p=O.169 (in 1) non-resp. 4,57 4.68 p=o.575 DLCO resp. 16,44 15.08 p=o.241 (in ml/mini non-resp. 14,06 12.42 p=o.515 dg) We conclude that all lung function parameters showed

changes in the expected direction (i.e. improvement in res- ponding patients) except DLCO which dropped in all patients irrespective of response to chemotherapy. Changes were eta- tistically non significant except for the rise of FEVl in responders which was borderline significant. *resp.=responders;**non-resp.=non responders (WHO criteria)

ImU~MY OF SCLC XWO@MTS WITH ANTI- NCABl MA6 12sC3: m#iT OF RESULTS BY INTERNALlUTtON. H.B. Kwa,A.H.M. Verhoeven, J. Hikes. Department of Tumor Biology and Medical Oncology, The Netherlands Cancer Institute and depmtment of Pohnonology, University Hospital Leiden, The Netherlands.

Three anti-NCAM mAbs were evaluated for immunoscintigrapbic use in a SCLC H69 xenogratl nude mouse model, nmnely mAbs 123C3, 123Afi and MOCl91. The affinity of these antibodies to the antigen, es expressed in the Ka value, was respectively 1.04, 0.43 and 1.16 lo9 M’. Each mAb wes evaluated for its usefulness for immunoscintigraphy of H69 tumors in nude mice. The best images were pmduced with radiolabeled mAb 123C3. The biolocelization study showed the highest fraction of injected dose per gram of tomor tissue for the same mAb (13.9% at day 7 after injection). Although MOCl91 was found to have similar in vitro affinity, the biolocalization and scintigraphic results were inferior (9.25% of ID/g of honor tissue at day 7). The dikence in results achieved with mAbs 123C3 end MOCl91 CM be explained by the internalization of mAb 123C3 into the tumor cells. The results witb mAb 123A8 were inferior to both other mAbs, possibly due to a lower affinity and lack of intexnaiization. Internalization for anti-NCAM mAbs has not bean described before. It was demonstrated in vitro using radiolsbclod antibodies, immuwfluoresccnce and electron microscopy. MAb 123C3 showed internalization into the tumor cells while the 2 other anti-NCAM mAbs did not. Intemalization of mAbs CM improve the results of immunoscintigmphy due to a better retention of the antibody in the tumor tissue.

256

USEFULNESS OF CINE CT-SCAN IN DIAGNOSING THE LUNG CANCER INVADING THE AORTA. T. Otsuka, J. Nakajima, T. Kohno, A. Furuse, N. Minami' . Dpts of Cardiothoracic Surgery and Radiology * . Faculty of Medicine, Univ of Tokyo, Tokyo, Japan.

CINE CT-SCAN(C-CT) visualizes the real-time movement of the lung cancer, and it is feasible to determine wheter or not it adheres to contacting tissues or organs. Preoperative C-CT was done in 5 cases with lung

cancer apparently contacting to the aorta. The slices were set at eight levels and the movie was taken at each slice for six seconds, synchronized with breathing. when the tumor moved synchronously with the aorta in the movie, it was suspected of Tb, i.e. direct invasion to the aorta, and preparation was done for the concomitant resection of the aorta. C-CT findings and operative findings were as follows:

C-CT OP Preparation

CASE 1 N N _ 2 P (descending) N T B

2 N' (arch) P CPB N _

5 P (descending) P TB

N or P represents negative or poitive findings of invasion to the aorta respectively. TB or CPB is temporary or cardiopulmonary bypass. He conclude that the C-CT is useful in evaluating

T-factor of the tuaor and preparing for the concomitant resection of the aorta.