updated virtual cat lab tuesday afternoon data
DESCRIPTION
labTRANSCRIPT
Protocol #11 2ACh10 ug/kg decrease in heart rate (bpm) Decrease in heart rate (bpm)Baseline 110 108.3Artery 8.! 8".#$ein 8#. 83.3Protocol #2 No Atropine Estimated [Atropine] (M)0 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 10.30.! 1.80." 33.11 #%.%! &.8" 8&.310 %".#!0 %&."0 %&.100 %&.Atropine Estimated [Atropine] (M)2 mg/kg 0.0001# 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 !0.! #0." 1!1 1%! 3!."" "310 &!!0 8""0 %3100 %3Atropine Estimated [Atropine] (M)5 mg/kg 0.0003" 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 .!0.! &.30." 13.!1 !0.3! 3!.8" ".10 &.!!0 8%.&"0 %.!100 %.!Atropine Estimated [Atropine] (M)10 mg/kg 0.000% 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 10.! 1.0." &.31 11."! 1&.&" !.110 #0.&!0 "".!"0 ".&100 %.8Atropine Estimated [Atropine] (M)20 mg/kg 0.00138 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 1.80.! !."0." #.11 .8! 1!."" !0.810 33.3!0 #&.%"0 8.&100 &"Protocol #3No Tubocurarine1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 &.80.! 1#.10." !%.%1 #&.!! "."" 8".#10 %#.#!0 %&"0 %&100 %&Tubocurarine2 mg/kg 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 100.! 1&.%0." 33.11 #%.3! 8.&" 8%.&10 %%.!!0 101.3"0 101.3100 101.3Protocol #4No Neostigmine1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 #.!0.! ".80." 1#.11 !#.! 3%.8" #10 &%.!!0 8&"0 %0.!100 8%.1Neostigmine0.1 mg/kg 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 1."0.! 1."0." ".!1 18.8! !8.8" ""10 "."!0 &0.8"0 &0.!100 &0.83Decrease in heart rate (bpm)108.883.%83.%2 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0#.1 ".!10.# %.%!0.8 !1.83".# !%.&"! "!.3&" .183.3 83.#8&." 88&." 88&." 82 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0! !.# #10.& 101%.3 1&3!.1 !%"".& "!&!.% 883. 8!8".& 8!8&.% 812 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0! 1."! 3.1.! .!10.# 11.%18.& 1%.!3".# 3".&"0 "!.3. &.3&" &.1&" &.12 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0# 0" 0.! !.18.3 .#1!." 8."!&.1 1%.!3%. 3#.!".! #%.!&0.8 8."&" &!.82 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0! 01 0! 03 3 "11 818 1030 18#" 3""3 #12 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0#.1 #.&10.# &.81. 1&.1#1. !8".! #".1&%.1 8.%%3.& 8#.3100 %1."100 %!.!100 %!.!2 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0#.1 .!10.# %.3!!.% 18.3&." 31.1&0.8 #8.!&& &3.8%." 88.1%".8 %.%%&.% %.%100 %.%2 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0#.! #.&.3 8.318.8 1.1!%.! !8#8 ##.&0.% 8.%8&." 8!.%8%. %0.&%1.& %!.!%3.8 %!.!2 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0#.! 0."8.# .&1#. 13."!!.% !.%3".# #0.%0.# "8!." %.#!." &3!." &3#. &3Decrease in heart rate (bpm)Averae !td Dev n !td Error Acet"lcholine into Arter"8".1& 1.1&18"%! 3 0.!#%83.%333333333 0."0#0&0%% 3 0.3&"#&Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 0."333333333 3.3080&0%38# 3 1.%0%%1"1!.3& 3.8#&"1001&3 3 !.!!130%!".!333333333 .8310""313 3 3.%#3%1!338.3333333333 10.#1#"&31!3 3 .01!8""&.3& %.03&&&11" 3 ".!1&38""&8.8 &.3%"&!& 3 #.!"&%3388&.3& .%"&!"0#1#& 3 #.01&&0#%0.3& .308%88!%&1 3 3.#!#%1%0.3& .308%88!%&1 3 3.#!#%1%0.3& .308%88!%&1 3 3.#!#%1Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 0! 0 3 0#.8 1.38"#0#1 3 0.810.% 1.01#88%1"" 3 0."8"%#"18.#333333333 1.!"0333!88% 3 0.&!1880331.! 1.%1"&!##01 3 1.100##"3."& 1.%13%83!%3 3 1.10"03%&0.%& !.083!00!13 3 1."0"%1#383."333333333 1."01110%%% 3 0.8&8.% "."%&3!0&8&& 3 3.!311#&8&.3 .0!!#"&%& 3 3.#&&0&&Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 03.!333333333 !."813#331%" 3 1.#%033%3#.1333333333 !.&%&0!!!!1 3 1.1#81"8."333333333 #.0#1#"188#3 3 !.33333331#.! ".33"&!8!88 3 3.080"8##!3."& 8.000!08330 3 #.18%!!##!."& 1!.1"#1#8811 3 &.01&!011"%." 1#."08!&3"01& 3 8.3&3""."333333333 13.13%3810103 3 &."80!"!8!.#333333333 11.%3#%!##8 3 .8%0"3%8!.#333333333 11.%3#%!##8 3 .8%0"3%Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 01.& !.081"%%%" 3 1.!018"0#!.! !.""3#!%0& 3 1.#!!3".! !.#3&%!1! 3 1."8!1%!8.&333333333 !.""3 3 1.#881011!.% #.130!"03&% 3 !.3331!!#.1333333333 #.301""0108! 3 !.#83"01138.1& 3.#&8%8#"!! 3 !.008"%!&"3."333333333 3.&8"%388%&! 3 !.18"81!88.3333333333 !.""#081&00 3 1.#"%%8&!."333333333 !.10!3!01 3 1."0&0!0Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 01.!& 1.101"1#10%" 3 0.3"%"%"1.1& 1.!"830"&3%! 3 0.&!#83!!.0333333333 !.0"0!03!#! 3 1.1838"##.!& !.1%3%310!!% 3 1.!&&.8333333333 #.0&!!3%0& 3 !.3"11!!&13.!& .%#!&1! 3 3.8#%#!1!0.#333333333 11.83%0"%#&3 3 .83"!8#331.%& 1".0#&0"0"& 3 8.8&!1%!#%."& 1&.30&8%800#" 3 %.%%!&1%".3333333333 1&.!#33"!08" 3 %.%""#"3Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 0"."333333333 1.%8"&8!8011 3 1.1##%!!10.&& 3.1"%#83#" 3 1.8!&8&0!1.! &."38"&"03! 3 #.3"!3%#38.%333333333 %.8&38&1!#3" 3 ".&008!!"". 10.!13!!&183 3 ".8%0%!&&.8 8.3!#383"3 3 #.80&!81%0.8 ".#003"#0% 3 3.!"!&%.1& #.31083%0"!1 3 !.#888#1%.# 3.%3##311# 3 !.!&1"33%.# 3.%3##311#Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 0.&& !.%%0"#0#!3 3 1.&!"8%#1!."333333333 #.800%%&1# 3 !.&0!0"8!#.8& &.##&3&0%00& 3 #.!%%3%.3 %.!3!""11101 3 ".330#1"%!."& 1!.#81!"33# 3 &.!088880.1 8.#8"8&00%# 3 #.8%%31%&%!.!& .0#"108!1"! 3 3.#%01##%%8 !.%103!##!! 3 1.80!&&8%8.& !.30"1!"18% 3 1.331"%%.# !.!0"30%111 3 1.30"1181Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 0#.3& 0.!88&"13# 3 0.1&.8 1.3!!8&"""" 3 0.&3&!1.3333333333 !.3"8&1%#!& 3 1.31&&%%!&.!& !.380&08%1 3 1.3&&"%8##.1333333333 #.11%8&0"#81 3 !.3&808#&.%333333333 3.""011&3% 3 !.0#%1!83.! #.1"81!#"&&3 3 !.#00%#38%.1 1.% 3 1.0%%""%1.3& 1.0#083!%%%& 3 0.00%!"!%1.& !.38%"0!%1 3 1.3&%13"Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3 0!.0& 1.%13%83!%3 3 1.10"03%"."333333333 3."%#%038003 3 !.0&""18&11.1 ".13%003 3 !.%&0#1!!!.8& #.0"010!8&%# 3 !.3383!83".0333333333 .0"83!&0! 3 3.#%&&&&1"&.8 !.&0""#%8"1& 3 1."!0#%%".8 3.#"%&8&&8# 3 1.%%%8#8.&& "."3#8!%1 3 3.1%&0#&!8."& ".#3&!1!" 3 3.13%1&8%%.#& #.3""838%%3 3 !."1#8##8Acet"lcholine into Arter" 8".1&Acetylch'line int' 83.%3333Carbach'l int' Art 8%.3Carbach'l int' (e %0.&0 0 0 0 0 00.1 ."33333 ! 3.!33333 1.& 1.!&0.! 1!.3& #.8 #.133333 !.! 1.1&0." !".!3333 10.% 8."33333 ".! !.0333331 38.33333 18.#3333 1#.! 8.&33333 #.!&! "&.3& 31.! !3."& 1!.% &.833333" &8.8 "3."& #!."& !#.13333 13.!&10 8&.3& &0.%& "%." 38.1& !0.#3333!0 %0.3& 83."3333 ."3333 "3."3333 31.%&"0 %0.3& 8.% 8!.#3333 8.33333 #%."&100 %0.3& 8&.3 8!.#3333 &!."3333 ".3333380828486889092Efect on Heart Rate from Chemical Injection into Cat ModelChemical Injected and WhereDecrease in Heart Rate (bpm)0 0 0 0 00.1 "."33333 .&& 10!.8& 10".1&0.! 10.&& 1!."3333 %&.#3333 %%.#33330." !1.! !#.8& 8#." 8&.!1 38.%3333 3%.3 %."& &1.!! "". !."& "1."& "!.%" &&.8 80.1 !%."3333 30."10 %0.8 %!.!& !0.03333 18.33330 10 20 30 40 50 60 70 80 90 1000102030405060708090100Atropine on Heart Rate Response to Acetylcholine0.0 mg/kg Atropine0.5 mg/kg Atropine1 mg/kg Atropine2 mg/kg Atropine5 mg/kg AtropineAcetylcholine (!"#!)Decrease in Heart Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on Heart Rate Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate (bpm)80828486889092Efect on Heart Rate from Chemical Injection into Cat ModelChemical Injected and WhereDecrease in Heart Rate (bpm)!0 %.1& %8 1.& 1#.!3333"0 %.# %8.& 1&.&& 1#.%3333100 %.# %%.# 1%.&& 1&.&33330 0 0 0 00.1 #.3& !.0& 10!.1& 81.&0.! .8 "."33333 %#.& &8.!33330." 1.33333 11.1 80.%3333 &1.#1 !&.!& !!.8& ".% 0.#3333! ##.13333 3".03333 #8.0& #.&&" &.%3333 "&.8 !.0& !8.%333310 83.! ".8 1&.% 1%.33333!0 8%.1 8.&& 1".!& 1&.!3333"0 %1.3& 8."& !!.83333 !1.#100 %1.& %.#& 31.3333 30.%&0 20 40 60 80 100 120020406080100120$bocrarine on Heart Rate Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate (bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate (bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate (bpm)decrease in heart rate (bpm)80828486889092Efect on Heart Rate from Chemical Injection into Cat ModelChemical Injected and WhereDecrease in Heart Rate (bpm)0 10 20 30 40 50 60 70 80 90 1000102030405060708090100Atropine on Heart Rate Response to Acetylcholine0.0 mg/kg Atropine0.5 mg/kg Atropine1 mg/kg Atropine2 mg/kg Atropine5 mg/kg AtropineAcetylcholine (!"#!)Decrease in Heart Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on Heart Rate Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate (bpm)80828486889092Efect on Heart Rate from Chemical Injection into Cat ModelChemical Injected and WhereDecrease in Heart Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on Heart Rate Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate (bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate (bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate (bpm)Tubocurarine0 20 40 60 80 100 120020406080100120$bocrarine on Heart Rate Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on Heart Rate Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate (bpm)Protocol #11 2ACh10 ug/kg increase in tensi'n (g) increase in tensi'n (g)Baseline 0 0Artery 0.& 0.#$ein 0 0Protocol #2 No tubocurarine1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 0.00.1 !.%0.! #.30." &.%1 1#.3! !".0" #".010 1.#!0 &&.1"0 8#.3100 8%.3tubocurarine0.5 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 00.! #."0." 31 .1! 8.3" !!10 3.#!0 "0"0 &.#100 &3."tubocurarine1 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 0.&0.! 1.80." !.81 #.3! &.8" 18.110 3".!!0 3%.""0 "%.1100 .%tubocurarine2 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 1."0.! 1."0." 3.11 %.!! 1#." !.%10 ##.!0 "%.!"0 &0.8100 &!.3tubocurarine5 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 00.! 00." 11 #.!! !.8" #.!10 %!0 1#."0 !%.!100 #1.&Protocol #3No Atropine1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 0.00.1 !."0.! #.0." %.31 1.0! !8."" #%.810 ".8!0 &%.#"0 8#.3100 88.8Atropine )h'uld ha$e n' e**ect5 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 !.80.! #.30." 8.%1 1.&! !8."" "0.!10 .!!0 &%.#"0 8&."100 88.Protocol #4No Neostigmine1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 0.00.1 !.80.! ".30." 10.31 1%.! 33.1" "".!10 &0."!0 8!.%"0 8%.&100 %0.0Neostigmine0.1 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 0.00.1 .00.! 10.&0." !0.1 3%.1! "3.#" &0.810 8!.!0 8#.3"0 8&."100 8%.03increase in tensi'n (g)00.%02 3increase in tensi'n (g) increase in tensi'n (g)0 0!.1 1.13." 3.#&. ".&13.% 13.8!" !".3##.# #!."1.8 !.1&".& &".%&%.! 83.%8.8 8".12 3increase in tensi'n (g) increase in tensi'n (g)0 00 1.#1.# 1.#3." !.8&. #.%10.# 10.#!!.! !!.%3".# 38.!"!.1 "0.&%.# 8.1.3 &0.82 3increase in tensi'n (g) increase in tensi'n (g)0 01.# 1.&1. 1.#!.1 !.1#.% 3.8.! &.31".3 1#.!!.# !8.###.# 38.#".! ".18.1 .12 3increase in tensi'n (g) increase in tensi'n (g)0.0 00.0 0.&0.0 !.1!.1 !.8!.8 3."%.& &.#1!." %.&1&.# 1&.#!".& !8."#3.1 #".8"#.! "!.82 3increase in tensi'n (g) increase in tensi'n (g)0 00 00.& 00.& !.13." 3."!.8 ".%%.& 1.&10.# !!.%13.! !3.!" "#3".3 0.#2 3increase in tensi'n (g) increase in tensi'n (g)0 0!.1 !.13." 3.".% &.313.% 1#.!!#.3 !#.%##.# ##.31.8 1.&".0# &".18".# 8.!88.% 88.%2 3increase in tensi'n (g) increase in tensi'n (g)0 0!.1 !.13." 3.8&. &.313.% 13.8!#.3 !3.%##.# ##.31.1 !.3&".& &."8".# 8&.%%0.3 8%.32 3increase in tensi'n (g) increase in tensi'n (g)0 01.# 1.&3." !.#.% .!11.8 11.1!1." 1%#0.3 3&.&"%.3 "3.&!.% &0.%8#.& 8388.% 8.%2 3increase in tensi'n (g) increase in tensi'n (g)0 03." 3.1.! "."13.% 11.1!1." !3."33.3 3#.3"0.& "3.3&!.% ".#&%.! &&.!81.3 81.&8" 8!#ncrease in tension ()Averae !td Dev n !td Error00.& 0.!"111#&8 3 0.1#"!%0 0 3 0#ncrease in tension ()Averae !td Dev n !td Error0 0 3 0!.0333333333 0.%018#%%"0 3 0."!08333.&333333333 0.#%3!88!8! 3 0.!8#8001&.0& 1.1%303"3##" 3 0.88&%%31# 0.!#"&"1311 3 0.1"!&"!"!".1 0.1&3!0"0808 3 0.1#3.%& 1.30"11813 3 0.&"3"1031.&& 0.3"1188#"8# 3 0.!0!&"88&.!333333333 0.&"&18&&&%# 3 0.#3&1!8!.#& !.830&&10!% 3 1.3%%8&.0& !.11!0!"03 3 1.!1%"#ncrease in tension ()Averae !td Dev n !td Error0 0 3 00.#& 0.808!%03&% 3 0.#&!.#333333333 1.&8%&8"83#" 3 1.03333333.1 0.30"""1!&" 3 0.!081.! 1.3"!&%!"8 3 0.&810!"%.& 1.!1!#3"""3 3 0.&!!.3& 0.#&!"81"! 3 0.!&!8#"13.& 1.#18%1%&&% 3 0.81%!13&"0.%333333333 1.0%!&!! 3 0.1&3#!8.3 1.01#88%1"" 3 0."8"%#"&!.8& 1.833%3%!%38 3 1.0"88!"3#ncrease in tension ()Averae !td Dev n !td Error0 0 3 01.!& 0."13101#3% 3 0.!%!&311. 0.! 3 0.11"#&01!.3333333333 0.#0#1#"188# 3 0.!333333#.3333333333 0.""0&"&0"#& 3 0.31&%&%&&.1 0.818"3"!&&! 3 0.#&!"811".8& !.01080#1"0% 3 1.10%38330 #.130!"03&% 3 !.3331!#0.&& 3.1%#!"%"" 3 1.8##!10!"&.1333333333 1.&03%1&0""% 3 0.%83&"&&.0333333333 1.00##"%1# 3 0."8118"#ncrease in tension ()Averae !td Dev n !td Error0 0 3 00.&333333333 0.&"0"""3#%% 3 0.#3333331.! 1.081"38! 3 0.!##%%8!.& 0."13101#3% 3 0.!%!&31".1& 3."10#0""8 3 !.0!&"310."& 3.&"0&8 3 !.1!31"!81.3& %.!!8%3%%8%& 3 ".3!8331!.#& 1".&03%!&3!! 3 %.0&3&.8 18."8"&!! 3 10.&30#8"3.!333333333 1".!&!%08"8 3 8.81&8#81"%.&& 10.8&&33"&83 3 .!&%8"1#ncrease in tension ()Averae !td Dev n !td Error0 0 3 00 0 3 00.!333333333 0.#0#1#"188# 3 0.!3333331.!& 0.&3&111#&% 3 0.#!""&1"3.&333333333 0.#0#1#"188# 3 0.!3333333.8333333333 1.&8%&8"83#" 3 1.033333310.! .!#%8!0#31 3 3.1&08%11#.1 &."3103%#"" 3 #.#18"!11&.1333333333 ".#38&"&3% 3 3.!"8#%3!3.0& 1".&!0""810& 3 %.0#8!"#".8 13.0#!!!#3"& 3 &."3011#ncrease in tension ()Averae !td Dev n !td Error0 0 3 0 0!.!333333333 0.!30%#010&& 3 0.1333333 0.13.8& 0.3"08"!%1 3 0.3& 0.!&.8333333333 1.!8"8!0101" 3 0.!38 0."1#.& 1.13"&81%! 3 0.""% 1!".% !.!&1"33383 3 1.311#8&& !#.1& 3.1#%"!01# 3 1.818% "3.0& !.3%!#%! 3 1.3&88" 10&."133333333 !."0010## 3 1.##3#3&3 !08".3 0.%"3%3%!01# 3 0.""0&"&1 "088.8& 0.0"&&3"0!% 100#ncrease in tension ()Averae !td Dev n !td Error0 0 3 0!.3333333333 0.#0#1#"188# 3 0.!3333333.8& 0.#0#1#"188# 3 0.!333333&.%333333333 0.8"0#%00"#8 3 0.#%1030&1#.8 1.#!0&&33 3 0.%"0#38"!"."& !."#8!01%80" 3 1.#&1!0"1#.3 3.3&&8%1"08 3 1.%"0!13&3.! !.#"83!"! 3 1."3%#80#&&.! 1.%#&%!!33# 3 1.1!3%818.%333333333 1.3#!88!#&1% 3 0.&&"3138%.# 0.8"##003" 3 0.#%3!883#ncrease in tension ()Averae !td Dev n !td Error0 0 3 01.%& 0.&3&111#&% 3 0.#!""&1"3.&333333333 1.##01!&"0# 3 0.8#"!#8!&.8 !.1%31&1!1%% 3 1.!!!81#.1& #.&18#038#% 3 !.&!#1&1!#."333333333 &."!3"18803 3 #.3#3&0""##.# %.##!%881" 3 ".#"1%111.1333333333 8."%&8&%"!8 3 #.%3%81#&"."& .#!%100"0&3 3 3.&118#!%8".8 3.#8!81#%"3" 3 !.01080#!88. 1."&1!33# 3 0.%0&3&&!1.83%3&1.0!11#%#ncrease in tension ()Averae !td Dev n !td Error0 0 3 0#.! 1."&1!33# 3 0.%0&3&&!&.#& !.8!1%3&88# 3 1.!%!#1".! #.881"%80%% 3 !.8183%!!8.0333333333 %.30#3##81 3 "."33&!3#0.3333333333 11.3!&1013!3 3 ."3%&0&8"8.!& 10.%31&"801 3 .311#"3#&3.333333333 8.!3#1&&!%3 3 #.%&8&3!"80.!333333333 3.10"313% 3 !.113&11883." 3.#%8&031# 3 !.0033308".3333333333 3."1188#"8#3 3 !.0!&"8&"!.1&3#"!1.!"131&3Base 0Acetylch'li 0.&Acetylch'li 0Carbach'l i &0.0Carbach'l i 0.00 0 0 0 0 00.1 !.033333 0.#& 1.!& 0.&33333 00.! 3.&33333 !.#33333 1. 1.! 0.!333330." &.0& 3.1 !.333333 !.& 1.!&1 1# .! #.333333 ".1& 3.&33333! !".1 %.& &.1 10."& 3.833333" #3.%& !!.3& 1".8& 1.3& 10.!10 1.&& 3.& 30 !.#& 1#.1!0 &.!3333 "0.%3333 #0.&& 3&.8 1&.13333"0 8!.#& 8.3 "&.13333 "3.!3333 3.0&100 8&.0& &!.8& &.03333 "%.&& #".80 0 0 0!.!33333 !.333333 0.810313 !.######3.8& 3.8& 1.##"0!8 3.#""""&.833333 &.%33333 !.8%"!&3 .0#####1#.& 1#.8 ".3#"!1 10.3333!".% !"."& %."!38"# 1&.%3333#.1& #.3 1&."1"" 31.1"""0 10 20 30 40 50 60 70 80 90 1000102030405060708090$bocrarine on eletal Mscle Response to Acetylcholine0.0 mg/kg T!ocrarine0.5 mg/kg T!ocrarine1 mg/kg T!ocrarine2 mg/kg T!ocrarine5 mg/kg T!ocrarineAcetylcholine (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)3.0& 3.! !#.&!308 #!.""""&."1333 &&.! 3!."133& "1."3&&88".3 8.%3333 #".#"131 "8.1&88.8& 8%.# !.%8"%1 "%.!"""acetylch'line by itsel*and in the presence '* atr'pine and e**ect '* carbach'l by itsel* and in the presence '*atr'pineAC+, AC+ -e' car!acho0.1 neo$tig0 0 0 0 00.1 1.%& #.! 4.2 4.80.! 3.&33333 &.#& 8.4 8.60." &.8 1".! 17.3 17.41 1#.1& !8.03333 28.8 29.2! !#."3333 #0.33333 43.3 44.6" ##.# "8.!& 65.1 60.110 1.13333 &3.3333 72.1 72.7!0 &"."& 80.!3333 79.3 78.4"0 8".8 83." 81.0 81.1100 88. 8".33333 83.8 80.40 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)Acetylcholine into Artery "ar!achol into Artery01020304050607080Efect on eletal Mscle from Chemical Injection into Cat ModelChemical Injected and WhereIncrease in $ention (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090$bocrarine on eletal Mscle Response to Acetylcholine0.0 mg/kg T!ocrarine0.5 mg/kg T!ocrarine1 mg/kg T!ocrarine2 mg/kg T!ocrarine5 mg/kg T!ocrarineAcetylcholine (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)Acetylcholine into Artery "ar!achol into Artery01020304050607080Efect on eletal Mscle from Chemical Injection into Cat ModelChemical Injected and WhereIncrease in $ention (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine on eletal Mscle Response With Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg #eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)