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Synchrotron Radiation Protein Crystallography Facility

User Committee Meeting

Core Facility Report

Yuch-Cheng JeanApril 27, 2011

I. Service Statistics and Research Outcomes

II. Training and Dissemination Activities

III. Beamline Development

Outline

I. Service Statistics and Research Outcomes

BL13B1 Service Statistics

83.3 %96716232282010-1

86.3 %196930059382009-3

85.6 %168620245382010-2

86.8 %87919533262009-2

88.1 %176428049312009-1

77.5 %123120937272008-3

81.4 %160423142262008-2

90.9 %172023845262008-1

88.5 %164921737242007-3

83.8 %233893326587403Sum

84.0 %164524650402010-3

89.5 %183022939232007-2

90.9 %109215527192007-1

77.8 %154119830172006-3

86.5 %157423131172006-2

76.7 %114213318132006-1

65.9 %109610213102005-3

% of BeamtimeUsed for Service

# of BeamtimeHours Delivered

# of TrainedUsers

# of Conducted Experiments

# of Visit User Groups

BL13B1Beamtime Period

Updated to 2010-3 period

BL13C1 Service Statistics

78.9 %91513527262010-1

75.3 %171815328272009-3

80.7 %79312221202009-2

63.8 %125715627262010-2

79.6 %126415825242008-3

67.4 %125314121152006-2

84.7 %166716928222008-2

81.1 %162517930212009-1

74.7 %195302360390304Sum

83.9 %164324140322010-3

82.0 %155217530192008-1

78.9 %147116433202007-3

57.7 %117914523162007-2

81.3 %92011521152007-1

58.3 %115415021112006-3

76.3 %111812716112006-1

% of BeamtimeUsed for Service

# of BeamtimeHours Delivered

# of TrainedUsers

# of Conducted Experiments

# of Visit User Groups

BL13C1Beamtime Period


11

15

25

4649

0

12

22

27

34

39

4

15

20

0

10

20

30

40

50

60

2005 2006 2007 2008 2009 2010 2011

Year

Num

ber

of U

ser

Gro

up

DomesticInternational

Growth of User Groups


User Group DistributionNational Chiao-Tung University (2)Department of Biological Science and Technology

National Health Research Institute (2)Division of Biotechnology and Pharmaceutical ResearchInstitute of Cellular and System Medicine

NSRRC (4)National Tsing-Hua University (5)Institute of Molecular and cellular BiologyInstitute of Bioinformatics and Structural Biology

Yuanpei University (1)Department of Biotechnology

National Chung-Hsing University (4)Institute of BiochemistryBiotechnology Center

Chung Shan Medical University (1)Department of Biomedical Sciences

HungKuang University (1)Department of Biotechnology

Academia Sinica (12)Institute of ChemistryInstitute of BiochemistryInstitute of Molecular BiologyGenomic Research Center

National Defense Medical Center (1)Institute of Biochemistry

National Taipei University of Technology (1)Institute of Organic and Polymeric Materials

National Taiwan University (7)Department of BiotechnologyDepartment of Life ScienceDepartment of Agricultural ChemistryInstitute of Biochemistry and Molecular BiologyGraduate Institute of Oral BiologyInstitute of Biotechnology

National Yang-Ming University (2)Institute of Genome Science

There are currently 49 domestic users and 27 international users

National Chiayi University (1)Department of Biochemical Science and Technology

Updated to 2011-2 periodNational Cheng-Kung University (2)

Department of Earth SciencesDepartment of Microbiology

National Tong Hwa University (1)Department of Chemistry

TAIWAN

National Sun Yat-sen University (1)Department of Marine Biotechnology

Chang Gung University (1)Chang Gung Molecular Medicine Research Center

Taiwan

Singapore

Hong Kong

Japan (7)Hokkaido UniversityKyoto UniversityNagoya University Osaka UniversityPhoto FactoryYokohama City University

Korea (1)Yonsei University

China (1)Institute of Cellular and System Medicine

Hong Kong (1)University of Hong Kong

Thailand (4)Mahidol UniversityNational Center for Genetic Engin. and Biotech.Suranaree University of Technology

Singapore (13)Institute of Molecular and Cell BiologyNan-Yang Technological UniversityNational University of Singpore

User Group Distribution

There are currently 49 domestic users and 27 international users


• Science• Nat. Chem. Biol.• Mol. Cell• Nat. Struct. Mol. Biol.• Angew. Chem. Int. Ed.• Plant Cell• Proc. Natl. Acad. Sci. USA• Curr. Opin. Struc. Biol.• EMBO J.• J. Am. Chem. Soc.• Nucleic Acids Res.• FASEB J.• Plant Physiol.• Mol. Cell. Bio.• Structure• J. Biol. Chem.• RNA• Biochem J.• J. Med. Chem.• Plos One• J. Mol. Biol.• …

Number of User Publications

0

10

20

30

40

50

60

2002 2003 2004 2005 2006 2007 2008 2009 2010

IF > 9.0 IF > 6.0 IF > 4.8 Total SCI

2006年核心設施開放後

2006年

核心設施開放後

2006年

核心

設施開放前

Top 10% SCI Publications (2010)

Top 10% SCI Publications (2011)

0

10

20

30

40

50

60

70

80

90

2001 2002 2003 2004 2005 2006 2007 2008 2009 2010

Growth of Protein Structures

Num

ber

of P

rote

in S

truc

ture

2006年核心設施開放後

II. Training and Dissemination Activities

2010.05.26~27 Workshop for Remote User Access

2010.08.09~13 2010 PX Training Course

2010 PX Training Activities

2011.01.17~21 2011 PX Winter School

2011.09.05~09 2011 PX Training Course

2011 PX Training Activities

2011 PX Winter School (1/17-21, 14 Trainees)

III. Beamline Development

1. Beamline Automation

2. Remote Access

3. Mail-in Service

YesYesNo13C1

YesYesNo13B1Mail-In Service

YesYesNo13C1

YesNoNo13B1Remote Access

YesYesYes13C1

YesYesNo13B1Automation

FY 2010FY 2009FY 2008BeamlineCapability

Tetative Delivery Target Year

How to use SAM to collect data for you

How to use Remote Access to collect data for you

How to use Mail-In Service to collect data for you

Future Plan

核心設施第三期計畫(2008.05.01 – 2011.04.30)組織

NRPGM計畫總主持人：王惠鈞核心辦公室主任：蔡明道

核心辦公室經理：袁佩宜

核心計畫主持人：陳建德

核心計畫共同主持人：袁小琀、蕭傳鐙

設施經理：簡玉成、趙俊雄

使用者委員會

詹迺立

王雯靜、伍素瑩、周三和

馬徹、孫玉珠、殷獻生

徐駿森、陳俊榮、楊啟伸

廖彥銓、廖淑惠、鄭貽生

生技類核心設施計畫(2011.05.01 – 2014.04.30)組織

NRPB計畫共同主持人：王惠鈞核心辦公室主任：陳仲瑄

核心辦公室經理：袁佩宜

國科會生物處承辨人：張絲珍

核心計畫主持人：陳建德

設施主任：簡玉成

設施經理：趙俊雄、陳彥儒

使用者委員會

王惠鈞

袁小琀、蕭傳鐙、詹迺立

王雯靜、伍素瑩、周三和

馬徹、孫玉珠、殷獻生

徐駿森、陳俊榮、楊啟伸

廖彥銓、廖淑惠、鄭貽生

Status of BL15A1

BL15A1 Beamline Schematics

•Energy range: 5.5 keV – 16 keV (wavelength 2.25 - 0.775 Å) •Photon beamsize at sample: ~ 250 µm (FWHM) •Beam divergence a sample: < 3 mrad (H) x < 1 mrad (V)•Flux at sample (ph/s) @ 1Å: > 1.5x1011 in 100 µm x 100 µm

BL15A1 Beamline Schematics

3-D Drawing of BL15A1

BL15A1 Protein Crystallography Station

MD2 Diffractometer

MX300HE CCD Detector

Huber Alignment Table

BL15A1 Tentative Time Table

4. Sub-system testing and integrated testing

Year

Working Item2009 2010 2011 2012 2013 2014

1. Long-lead time procurments of major equipments

2. Completion of final design

3. Procurment and installation

5. BL commissioning

TPS-PXCurrent

Original

Status of TPS Phase-I PX Beamline

2008.04.23 The 1st PXIG-WG meeting2008.06.11 The 2nd PXIG-WG meeting2008.07.06 Submit LOI to NSRRC2008.08.15 Receive format review comments2008.09.02 PI/Co-PI meeting2008.09.03 Submit revised LOI to NSRRC2008.12.31 Receive BSC review comments2009.01.08 Present the Science Plan in 2009 SAC Meeting2009.03.13 Receive SAC review comments2009.03.17 Beamlie integration meeting (NTHU merged into PXIG)2009.04.16 Receive CDR-1 format2009.04.23 The 3rd PXIG-WG meeting2009.05.01 Start working on the CDR-1 with the help from NSRRC2009.10.29 The 4th PXIG-WG meeting2010.01.14 Present the Beamline Plan in 2010 SAC Meeting2010.04.14 Receive SAC review comments2010.11.23 Submit CDR to NSRRC2011.02.14 Present the PX User Program in 2011 SAC Meeting2011.03.08 Receive SAC review comments2011.03.31 Receive CDR review comments

Action Items

Users’Requirement

Current Design

Revised Design

Energy Range (keV) 5.5 – 20.0 5.5 – 15.5 5.7 – 20.0

Vertival Beam Divergence (mrad) ~0.1 0.092 0.1

Horizontal Beam Divergence (mrad) ~0.5 0.14 0.5

Horizontal Beam Size (µm) ~50 97 57

Vertical Beam Size (µm) ~50 45 50

Total Flux @1Å(Phs/sec)

> 4E12 4.7E12 3.8E12

Defined Flux after A 50 µm Aperture @1Å(Phs/sec)

> 1E12 1.5E12 1.3E12

Flux Density after A 50 µm Aperture @1Å(Phs/sec/µm2) > 4E8 7.6E8 6.6E8

Defined Flux after A 5 µm Aperture @1Å(Phs/sec)

> 2E10 1.2E10 1.3E10

Flux Density after A 5 µm Aperture @1Å(Phs/sec/µm2) > 1E9 6.1E8 6.6E8

Revised Performance Evaluation

SRF

SRFSRF

SRF

Injection 01

09

TPS第一期周邊實驗設施位置規劃

25(74m)

23(75m)

43

高解析非彈性軟X光散射(Soft RIXS)

X光奈米探測(Nano-probe)

同調X光散射(Coherent ScatteringSAXS)

微米X光蛋白質結晶學(µ Protein Crystallography)

時間同調X光繞射(Temporal Coherent XRD)

次微米軟X光能譜(µ soft X-ray)

次微米X光繞射(Sub-μ XRD)

21(73m)

3341

053 GeV, 500 mA

24-cell DBAID: 12m X 6, 7 m X 18

15 ID and 24 BM available

Total beamlines:22 ID (odd #)24 BM (even #)

102年完成103年完成

IU22 x 3

IU22 (1+1) x 2

EPU48 (1+1) x 1

U50 x 1

以四年期「特別申請額度專案計畫」方式編列概算

TPS第一期周邊實驗設施建造經費與時程規劃

100年

高解析非彈性軟X光散射

次微米軟X光能譜 (德國MPI)

101年 102年 103年

年度經費

(總經費為19.56億 )1.56 億 3 億 5 億 5 億

一般計畫預算

104年

5億

微米X光蛋白質結晶學

同調X光散射

次微米X光繞射

X光奈米探測

時間同調X光繞射

TPS出光 TPS試車完成

光束線與實驗

站建造時程

年度

TPS-PX-BL Tentative Time Table

FY

Working Item99 100 101 102 103 104

TPS Installation

TPS Commissioning

1. Completion of conceptual design

2. Engineering design of major componetsand enclosure

3. Long-lead time procurments of major componets and enclosure

4. Completion of final design

5. Procurment and installation

6. Sub-system testing and integrated testing

7. BL commissioning

Status of TPS Phase-II PX Beamline

TPS周邊實驗設施之長程規劃

Phase I100-104 年經費:19.56億

7 座ID光束線/實驗站

高解析非彈性軟X光散射次微米軟X光能譜 (德國MPI)微米X光蛋白質結晶學同調X光散射時間同調X光繞射次微米X光繞射X光奈米探測

Phase II105-110 年經費:30億

Phase III

ID:

BM:

年

億

4座ID17座BM

105 106 107 108 109 1105 5 5 5 5 5

11座ID光束線/實驗站7 座BM光束線/實驗站

100 101 102 103 1041.56 3 5 5 5

TLS 搬遷TPS

07A 13B

16A01B01C17C

24A

11 14 17 19 22 25752

23A

diagnostic

13A

新建 ID 0 1 1 1 2 2

15A

Thank You for Your Attention

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