using the fst to correlate depressive-like behavior and estrogen levels in three strains of mice
DESCRIPTION
This presentation will probably involve audience discussion, which will create action items. Use PowerPoint to keep track of these action items during your presentation In Slide Show, click on the right mouse button Select “Meeting Minder” Select the “Action Items” tab - PowerPoint PPT PresentationTRANSCRIPT
Using the FST to Correlate Depressive-Like Behavior and Estrogen Levels in Three Strains of Mice
Kunwal Nasrullah
Columbia University, College of Physicians and Surgeons
D. Toran-Allerand M.D., Sc.D.
Imam Nethrapalli, PhD
Key TermsDepression-“symptoms meeting established clinical threshold criteria
for depression as measured by validated questionnaires or standardized psychiatric interviews”¹ vs. depressive-like behavior
Forced Swimming Test (FST)-a behavior test that serves as a rodent model for depressive-like symptoms in humans.
Estrogens- 17β-estradiol vs. 17α-estradiol
Mice strains: BALB/c, 129/SvEv, and c57BL/6J
Intact vs. Ovariectomized (OVX) vs. Adrenalectomized (ADRX)
What I do…
I am a double-blind scorer of mice FST conducted by my co-mentor, Imam Nethrapalli. Our ongoing project is to test the hypothesis that 17α-estradiol is produced locally in the brain and that it has antidepressant activities in mice.
What I see…
http://www.biobserve.com/products/fst/acquisition_movie.html
Computer Image of FST
http://62.193.209.148/en/content.php?content.66
Things that I look for…
During an FST scoring, three actions can be observed:
swimmingclimbing/scratching along the sides of the container floating/immobility: no active movement
HINT: The surface of the water is a good indicator of movement or immobility.
Possible ResultsMy results can help to validate
previous findings of my mentor.
It is also possible that my results will negate the findings of my mentor.
In either case, it is necessary to certify the antidepressant effects of 17α-estradiol in mice.
These results will be used in a paper.
Future PlansI will continue to score the FST
relevant to the study and learn lab techniques related to these behavior studies.
We will add results of an Open Field Test (a 3-by-3 feet square made of melamine which allows researchers to gauge the anxiety of mice and rats) to the results of the FST to support the lab’s work.
http://www.biochemj.org/bj/389/0593/bj3890593f01.gif
Open Field Test
References(2007). Bioserve: solutions for your science. Retrieved August 2, 2007 from
http://www.biobserve.com/products/fst/acquisition_movie.html.
(2006). Cheshire wildlife trust. Retrieved August 2, 2007 from http://www.wildlifetrust.org.uk/cheshire/IMAGES/watch_harvest_mice.jpg.
¹ Frasure-Smith N, Lesperance F, Talajic M, et al. Depression following myocardial infarction. Impact on 6-month survival. JAMA. 1993;270:1819–25.
Porsolt et al., 1978 R.D. Porsolt, G. Anton, N. Blavet and M. Jalfre, Behavioural despair in rats: a new model sensitive to antidepressant treatments, European Journal of Pharmacology. 47 (1978), pp. 379–391.
Toran-Allerand CD, Tinnikov AA, Singh RJ, Nethrapalli IS. 2005. 17alpha-estradiol: a brain-active estrogen? Endocrinology, 146:3843–3850.
van Meer, P., & Raber, J. (2005). Mouse behavioral analysis in systems biology. Biochemical journal, 389. Retrieved August 1, 2007, from http://www.biochemj.org/bj/389/0593/bj3890593.htm.
Villalba, Shannon. (2005). The behavior core. Retrieved August 1, 2007 from http://www.psychiatry.uc.edu/BehaviorCore/HTML/openfield.html.
Acknowledgments
Dr. Sat
HCS
HCS staff
Dr. Toran-Allerand
Dr. Imam Nethrapalli
Caitlin
Columbia University, College of Physicians and Surgeons
MSKCC
And you!