UWRF GomashiAnnotation

Matt SutterGabby Field

Megan Kreitzman

Annotations• Table 3 genes 18-27• Gabby Field 18-20, Matt Sutter 21-23,

Megan Kreitzman 24-27 • All genes FWD and similar to Halo or Liefie

Pham Map Genes 18-27• Our map matches

perfectly with the maps of other G phages. No genes are missing or added in this clip of genes.

• Our section of genes enable Gomashi to attach to hosts.

Gomashi-draft

Hope

Liefie

Halo

Confusion/Debate• Class -1 debate• LO- Listing length of longest • Good collaboration

Multiple starts?

Write annotation

All C.P. included?

Better blast match?

LO?

Change start

OK overlap

Good SD score

= no = yes

Gene 18• Start Changed!• Possible start @ 16,650 SD score of 462

matches Liefie gp18 1:1 • Called start @ 16,641-18,404 (FWD) SD score

of 378 and matches Liefie gp18 4:1• Phamily: 4102, members: 38 • Pham shared with K1-K5• Function: Minor tail protein

Gene 19• Possible start @ 18,359 with an overlap of 44,

LO, SD score of 294 and a blast of 16:1• Called start @ 18,404-18,865 (FWD) best SD

score of 609, overlap of 1 bp, matches Halo gp19 1:1

• Phamily: 1333, members: 32 • Pham shared with K1-K5• NKF

Gene 20• Possible start @ 18,658 with an overlap of 206• Called start @ 18,862-19,998 (FWD) best SD

score of 525, gap of 2 bp, matches Halo gp20 1:1

• Phamily: 3204, members: 33 • Pham shared with K1-K5 • Function: Minor tail protein.

Gene 21• One plausible start. Called @ 20,009-20,443

(FWD) good SD score of 483, overlap of 1, is LO, and 1:1 match with Halo gp21

• 48.1 % threshold shows that gene most likely originated from cluster K

• phamily: 4430 members: 32 • Pham shared with K1-K5• NKF

Gene 22• One plausible start called @ 20,442-22,836

(FWD) good SD score of 462, is LO, matches Halo gp22 1:1

• Phamily: 3208, members: 12 • Pham unique to G• NKF

Gene 23• One plausible start called @ 22,851-23,171

(FWD) good SD score of 735, L.O, matches Halo gp23 1:1

• Pham: 1371, members: 12• Pham unique to G• NKF

Gene 24• Possible start @ 23,168 matches Halo 3:1• Called start @ 23,174-23,356 (FWD) good SD

score of 378, gap of 2, matches Halo gp24 1:1 • Phamily: 1372, members: 12• Pham unique to G• NKF

Gene 25• Possible start @ 23,353 matches Halo 33:32,

38 bp overlap• Called start @ 23,356-23,631 (FWD) best SD

score of 525, overlap of 1, matches 32:32 with Halo

• Phamily: 1373, members: 12• Pham unique to G• NKF

Gene 26• Possible start @ 23,643 may not include all

c.p., matches Halo gp26 1:5. • Called start @ 23,631-23,825 (FWD) includes

all c.p. is L.O, good SD score of 441, matches Halo gp26 1:1

• Phamily: 3209, members: 12 • Pham unique to G• NKF

Gene 27• Possible start @ 23,871 with a SD score of 180.• Called start @ 23,886-25,202 (FWD) better SD

score of 378, matches Halo gp27 1:1 • Phamily: 4284, members: 423• Pham shared with A1-A10, B1-B5, C, D, E, F1, F2, H1, H2, I1, I2, J, K1-K5, L1-L3, M, N, O, P, Q, R, S, and T • Function Lys A

Similar G Locations

• Gomashi-River Falls, Wisconsin• Halo- Pittsburgh, Pennsylvania• LieFie- Durban, South Africa • BPs- Pittsburgh, Pennsylvania • Hope- Atlanta, Georgia

Impact• Gene 18 change • Few G phages are sequenced• Annotation Experience• Thank you!