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※基础研究 食品科学 2016, Vol.37, No.23 57
Vacuum Freezing Properties of Blanched Apple Slices
WANG Haiou, FU Qingquan, CHEN Shoujiang, WANG Rongrong, ZHANG Wei, YANG Ping(School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China)
Abstract: The vacuum freezing properties of blanched apple slices were investigated by comparing with traditional refrigerator freezing. Results showed that after 40 min vacuum freezing, a mass loss of 27.5% and the lowest frozen temperature of -27.6 ℃ were achieved in apple slices pretreated by blanching, while a mass loss of 22.9% and the lowest
frozen temperature of -26.5 ℃ were obtained in non-blanched apple slices. The total process of vacuum freezing was divided into low pressure flash evaporation stage, ice crystal formation stage and deep freezing stage. Vacuum freezing caused less microstructural changes and less significant cell disruption and contributed to smaller thawing loss and weaker relative electrical conductivity in frozen-thawed apple slices in contrast to refrigerator freezing. Blanching pretreatment before freezing caused more severe damage to cell microstructure in apple slices than non-blanching pretreatment, resulting in a significant increase in thawing loss and relative electrical conductivity in frozen-thawed apple slices.Key words: vacuum freezing; blanching pretreatment; apple slices; micro-structure
热烫处理苹果片真空冻结特性
王海鸥,扶庆权,陈守江,王蓉蓉,张 伟,杨 平
(南京晓庄学院食品科学学院,江苏 南京 211171)
摘 要:本研究与传统冰箱冷冻相比较,研究了热烫处理苹果片的真空冻结特性。真空冻结40 min后,经热烫处理
的苹果片冻结最低温度达-27.6 ℃,质量损失为27.5%,而未经热烫处理的苹果片的冻结最低温度和质量损失分别
为-26.5 ℃、22.9%。依据温度变化,苹果片真空冻结过程可分为:减压闪发段、冰晶形成段、深层冻结段。相对
于冰箱冷冻而言,真空冻结苹果片对组织微观结构改变小,冻融后汁液流失率低,电导率低,另外,冷冻前热烫处
理会引起更大的组织结构损坏,显著增加冻融后的汁液流失率和电导率。
关键词:真空冻结;热烫处理;苹果片;微观结构
DOI:10.7506/spkx1002-6630-201623010中图分类号:TS255.3 文献标志码:A 文章编号:1002-6630(2016)23-0057-07
引文格式:
WANG Haiou, FU Qingquan, CHEN Shoujiang, et al. Vacuum freezing properties of blanched apple slices[J]. 食品科学,
2016, 37(23): 57-63. DOI:10.7506/spkx1002-6630-201623010. http://www.spkx.net.cn
WANG Haiou, FU Qingquan, CHEN Shoujiang, et al. Vacuum freezing properties of blanched apple slices[J]. Food Science,
2016, 37(23): 57-63. (in English with Chinese abstract) DOI:10.7506/spkx1002-6630-201623010. http://www.spkx.net.cn
收稿日期:2016-07-24
基金项目:国家自然科学基金青年科学基金项目(31301592);常州市科技支撑计划项目(CE20152017);
江苏省教育厅自然科学基金项目(15KJB550008);南京晓庄学院人才引进项目(2013xzrc04)
作者简介:王海鸥(1978—),男,副教授,博士,主要从事食品冷冻与干燥加工技术研究。E-mail:[email protected]
Freezing has become one of the most important unit
operations in food processing and preservation[1]. And it is the
necessary process for food vacuum freeze-drying[2]. Usually,
freezing consists of three stages[3-4]: precooling stage, phase
transition stage and tempering stage, during which the
sensible and latent heat from food product are removed by the
traditional refrigerator cooling system. As a very effective and
clean cooling technology, vacuum cooling is characterized by
the rapid evaporation of the water in the product itself, and
quick removal of the heat contained in the product[5-7]. It was
widely used in the processing of fruits, vegetables, meat, fish,
sauces, soups, bakery, and ready meals[8-9].
The vacuum cooling turns into vacuum freezing if the
vapor pressure in the vacuum chamber drop below 0.6 kPa,
58 2016, Vol.37, No.23 食品科学 ※基础研究
i.e., the saturation pressure of water at 0 ℃. So vacuum
freezing is known as a new special freezing technique that is
attracting more and more researchers’ interests. And some
studies on vacuum freezing of food have been reported
recently. Cogné et al.[10] developed a numerical simulation
to model heat and mass transfer during vacuum freezing
of puree droplet. Chen et al.[11] studied the morphological
changes of water during vacuum cooling and vacuum
freezing, finding that the liquid water inside the vessel in a
vacuum cooling system was frozen into two layers: irregular
porous layer on the top and dense layer on the bottom. In
order to simplify the food freeze-drying process and shorten
the drying time, the author proposed a novel freeze-drying
technology in which the traditional air-freezing or plate
freezing process of food product was replaced by vacuum
freezing conducted in the same vacuum freeze dryer[12]. And
some laboratory experiments were performed on fruits and
vegetables with this novel freeze-drying technology. Zhang
Haifeng et al.[13] analyzed the process characteristics of water
vaporization and solidization, the organizational structure and
mass loss during the vacuum freezing of fresh mutton.
In the freezing or drying process of fruits and
vegetables, blanching pretreatment is generally applied
in order to undermine and suppress the enzyme activity,
prevent nutrients loss, retain original colors and taste, reduce
bacterial contamination, and so on[14-19]. There is few report
on the vacuum freezing properties of fruits and vegetables
with blanching pretreatment. The main objectives of the
current study are to evaluate the vacuum freezing properties
of blanching-treated apple slices in contrast with traditional
refrigerator freezing method, including mass loss, temperature
variation, thawing loss, relative electrical conductivity and
micro-structure of the tissue in the apple materials. Due
to the fact that there have been lots of reports about the
influence of blanching pretreatment on the sensory and
nutritional qualities of apples[14-19], so these quality indices
were not involved in this experiments. This study aimed to
provide basic knowledge for improving the effect of vacuum
freezing in fruits and vegetables and promoting the practical
application of this novel freezing technique.
1 Materials and Methods
1.1 Materials
Fresh Fuji apples were obtained from a farm located
in Yantai, Shangdong, China. The apples were selected
according to apparent color and size. Then, the selected
apples were washed, pitted and cut into 3 cm × 3 cm × 0.5 cm slices. The initial moisture content of these apple
slices was (86.34 ± 0.52)% (mf). Some apple slices were
blanched for 1 min in 95 ℃ distilled water heated by an
induction cooking plate and then cooled quickly to room
temperature using cold distilled water according to the
method of Wang Yuchuan et al.[16]. The blanching parameters
were chosen on the basis of the literatures[17-19]. And it was
also verified by the pre-experiments that the chosen blanching
parameters can suppress the enzyme activity and retain good
colors for the fresh slices. The non-blanched apple slices
were taken as the control group.
1.2 Instruments and equipment
50F vacuum f r eeze d rye r N ingbo Sc i en t z
Biotechnology Co. Ltd.; BCD-182DTB freeze refrigerator
Hefei Meiling Co. Ltd.; YNK/TH-50 constant temperature
and humidity chamber Suzhou Unique Environmental
Test Equipment Co.; FE38-Standard conductivity meter
Mettler-Toledo Instruments Co. Ltd.; HNY-1102C shaker
Nuoji Instrument Co. Ltd..
1.3 Methods
1.3.1 Vacuum freezing and refrigerator freezing
A laboratory-scale vacuum freeze dryer and a freeze
refrigeratorwere used for the freezing operation of apple
samples which were divided into 4 groups (group A, B,
C, D). Apple slices of group A and group B were selected
from the blanched slices, and those of group C and group D
from the non-blanched slices. Group A and group C were
performed vacuum freezing operation for 40 min in the
vacuum freeze dryer. The refrigeration units of the freeze
dryer were switched on half an hour earlier to reduce the
cold trap temperature below -50 ℃, then apple slices of
group A and group C were put into the freeze dryer at the
same batch ensuring the two groups’ vacuum freezing were
performed under the same operation parameters such as the
environmental pressure and the cold trap temperature. Apple
slices in group B and D were put into the freeze refrigerator
to perform traditional freezing for 4 h at -30 ℃.
1.3.2 Mass loss analysis in vacuum freezing
Three slices of apple samples in each group were
randomly selected and individually weighed before and after
vacuum freezing to determine the mass loss (ML), taking
the average value of 3 slices as the final result. ML was
calculated as the percentage loss of initial weightmass using
the formula (1).
※基础研究 食品科学 2016, Vol.37, No.23 59
ML/%= ×100m0-m1m0
(1)
where m0/g and m1/g were the weight of apple slice
before and after vacuum freezing, respectively.
1.3.3 Sample temperature analysis in vacuum freezing
The temperature of the geometric center of apple
slices in each group was measured using the thermocouple
temperature sensors of the vacuum freeze dryer to determine
the sample temperature variation during vacuum freezing.
1.3.4 Thawing loss analysis
After freezing, 3 frozen slices were taken out from each
group, separately placed into a high-density polyethylene bag
and thawed in a constant temperature and humidity chamber
maintained at (20 ± 0.5) ℃ and (70 ± 5)% relative humidity
until the temperature in the geometric center of the samples
reached 4 ℃. Each thawing experiment was undertaken in
triplicate. Then thawing loss (TL) was calculated according
to the formula (2).
TL/%= ×100m2-m3
m2 (2)
where m2/g was the weight of samples before freezing;
m3/g was the weight of samples after thawing.
1.3.5 Relative electrical conductivity analysis
The method was based on Lebovka et al.[20]. The
relative electrical conductivity (REC) of fresh slices (before
blanching), blanched slices (after blanching), and freeze-
thawed slices in group A, B, C, D (after freeze-thawing)
were all measured with the following method. Ten small
apple slices with 1 cm in diameter were obtained from each
group using the 1 cm-diameter hole puncher, then were
washed twice with deionized water, placed in the Erlenmeyer
flask containing 100 mL deionized water, and measured
the initial conductivity E0 with the conductivity meter. The
second conductivity E1 was also measured after shaking
the Erlenmeyer flask for 1 h at temperature of 15 ℃ in the
shaker. Subsequently, the Erlenmeyer flask was boiled for
15 min on the electric furnace, then cooled down to the room
temperature and added with deionized water until achieving
the original weight before boiling. Finally, the third conductivity
E2 was measured. All the measurements were undertaken in
triplicate. REC was calculated as the formula (3).
REC/%= ×100E1-E0
E2 (3)
1.3.6 Micro-structure analysis by light microscope
The method was modified from that of Ignat et al.[21].
Some of the fresh, blanched and freeze-thawed slices in
group A, B, C, D were taken for micro-structure analyses by
light microscope. All the prepared apple slices were fixed in
Formalin acetic acid alcohol (FAA) solution (90% ethanol,
5% acetic acid, 5% formalin) for 3 d. After fixation, gradient
elution with 30%, 50%, 70%, 90% and 100% ethanol was
performed for 15 min in each ethanol concentration. Then
the slices were processed by an automatic histoprocessor
to embed the tissue in paraffin which was cut into 5 μm
paraffin-tissue slices with a tissue slicer. The paraffin-tissue
slices were baked to remove paraffin, stained with Safranin
O/Fast Green, and finally were sealed in glass slide to be
ready for microscope imaging.
1.4 Statistics
Statistical analysis of variance (ANOVA) was performed
using SPSS 20.0 software. Tests of significant differences
between means were determined by Duncan’s multiple range
tests at a significance level at 0.05 (P<0.05).
2 Results and Discussion
2.1 ML analysis in vacuum freezing
00
10
20
30
10 20Time/min
30 40
ML
/%
group Agroup C
group A. blanched apple slices; group C. non-blanched apple slices. The same below.
Fig. 1 ML of apple slices after different times of vacuum freezing
During vacuum freezing the water in the apple slices
evaporated from liquid to vapor along with the performing
time causing the result of continual ML. As shown in Fig. 1,
ML in group A (27.5%) after vacuum freezing for 40 min was
significantly higher than that of group C (22.9%, P<0.05).
Due to the blanching pretreatment, the ML in group A was
increased by 32.35%, 21.85%, 20.26%, 20.00% at 10, 20, 30,
40 min of the vacuum-freezing performing time compared
with that in group C, respectively. Water evaporation rate (ML
rate) varied with a slowing down trend. The first 10 min was
the rapidest and main evaporation stage, contributing to more
than half of the total ML in 40 min.
During the vacuum cooling and freezing process, water
evaporation in apple slices was driven by the difference
between the water vapor pressure on materials and the
60 2016, Vol.37, No.23 食品科学 ※基础研究
pressure in the vacuum chamber. ML and frozen temperature
are the main indicators of vacuum freezing performance which
is affected by some factors including the initial temperature
of the material, moisture status and micro-structure of
the inner tissue, vacuum pressure, vacuum performing
time, the cold trap temperature and so on[10-11]. In our
experiments, vacuum freezing operations of group A and C
were performed at the same batch in the same equipment,
ensuring that the material initial temperature and the vacuum
processing conditions of the two groups were consistent. The
significant difference of ML between group A and C may be
mainly caused by the moisture status and micro-structure of
material tissue. This might be due to the fact that blanching
pretreatment resulted in some changes to the cell wall micro-
structure, contributing to the more ML in group A.
ML was inevitable phenomenon of vacuum freezing,
which lead to different results for food different processing.
ML was undesirable for the manufacturers if the vacuum-
frozen materials were used as instant freezing products
for the preserving purpose, and should be controlled as
small as possible to reduce the economic losses. However,
ML was desirable if the vacuum-freezing processing was
used as the purpose of freezing stage in freeze-drying
technology, and should be controlled as more as possible
to obtain the maximum dehydration mass and the lowest
freezing temperature in favor of enhancing freeze-drying
performances.
2.2 Sample temperature analysis in vacuum freezing
Sample temperature variation was caused by the phase
change of the moisture in the apple slices themselves,
including evaporation from liquid to vapor and freezing from
liquid to solid ice[10]. As shown in Fig. 2, the vacuum freezing
process can be divided into 3 stages according the change
curve of sample temperature which presented a similar trend
in the studies of Zhang Haifeng et al.[13].
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40Time/min
-30-25-20-15-10-505
10
Tem
pera
ture
/
group Agroup C
Fig. 2 Temperature changes of apple slices during vacuum freezing
The first stage: pressure-reducing and flashing stage.
The pressure in the vacuum chamber dropped to saturation
vapor pressure at the initial temperature of apple slices after
2 min of vacuum pumping, reaching to the flash-point of
water evaporation. Then rapid evaporation and boiling status
of water in apple slices happened, removing large quantity
of heat from samples themselves and leading to a rapid
reduction in sample temperature (dropped below -10 ℃).
The temperature curves of group A and C almost overlapped,
showing no obvious difference in slices temperature variation
caused by the blanching pretreatment.
The second stage: ice formation stage. Apple slice
samples turn into a super-cooling sate due to the happening
of the flashing evaporation. And ice crystals in samples were
gradually formed releasing a lot of latent heat, which slowed
down the temperature reduction rate of samples caused by the
removed heat of water evaporation. This stage lasted about
10 min, the temperature curve dropped with a slighter slope
compared with the first stage. Moreover, the temperature
curves of group A and C slightly separated showing that the
blanched samples had a lower temperature.
The third stage: deep freezing stage. Water evaporation
and freezing in slices get on simultaneously along with
the continuation of vacuum freezing. And the temperature
curves slowly leveled off at the limit frozen temperature at
-27.6 ℃ in group A and -26.5 ℃ in group C, following by
a slight rise. At this stage, most of water in the slices was
frozen and water evaporation gradually weakened. Even at
the later stage, sublimation drying happened due to the heat
transferring from ambient environment to the slices, which
contributed to the slight rise in slices temperature.
It is well known that vacuum freezing has an amazing
freezing rate in contrast to traditional refrigerator freezing,
which was verified by the above analyses of sample
temperature in vacuum freezing. And the temperature
variation and ML variation of apple slices showed a close
relation in Fig. 1 and Fig. 2. The more and faster ML, the
more and faster temperature reduction, which reflected
the energy conservation principle during vacuum freezing
process in some sense.
2.3 TL analysis
TL is an important indicator for the integrity in the cell
tissue of frozen products. Thawing experiments of samples
in this study were performed under the same temperature
and humidity. TL of the 4 groups was presented in Fig. 3.
TL in group B was highest (27.67%), followed by group
D (24.94%), group A (21.87%) and the lowest group C
(10.71%). The difference between any two groups in TL
※基础研究 食品科学 2016, Vol.37, No.23 61
was significant except group B and D (P<0.05). It can be
concluded that blanching pretreatment caused more TL in
terms of the same freezing method. Many available studies
revealed that a series of changes in tissues of fruits and
vegetables will happened during blanching pretreatment,
including degeneration of inner cell protoplasm, increase in
cell membrane permeability, extracellular and intracellular
water exchange, increase in tissue elasticity and toughness
and so on[22]. In this study, blanching pretreatment caused
about 104% and 11% increase in TL for the vacuum freezing
and the refrigerator freezing, respectively. This was probably
due to micro-structure damage on cell tissue of apple slices
caused by the blanching pretreatment.
c
a
c
b
A B C Dgroup
0
10
20
30
TL
/%
group A. vacuum-frozen apple slices with blanching pretreatment; group B.
refrigerator-frozen apple slices with blanching pretreatment; group C: vacuum-
frozen apple slices without blanching pretreatment; group D: refrigerator-
frozen apple slices without blanching pretreatment. The same in Fig. 4.
Fig. 3 TL of frozen-thawed apple slices from 4 groups
And TL in the refrigerator freezing method were
significantly higher than that in the vacuum freezing method.
This was probably due to the formation of larger ice crystals
in the extracellular or intracellular space because of extremely
slow freezing rate in refrigerator freezing, leading to more
disruption of cells[23-24].
2.4 REC analysis
contr
ol
blanc
hing
thawing
in gr
oup A
thawing
in gr
oup B
thawing
in gr
oup C
thawing
in gr
oup D
group
010203040506070
RE
C/% a
b
cd
b
c
Fig. 4 Relative electrical conductivity of apple slices after different treatments
REC is an important indicator to measure permeability of
cell membrane. Higher REC value represents greater damage
of membrane intactness. The REC results were shown in Fig. 4.
After blanching pretreatment and/or the freeze-thawing
treatment in all groups, the RECs in the slices were increased
with different degrees compared with the raw fresh slices. A
similar difference trend of REC in group A, B, C and D was
observed in Fig. 4 as TL in Fig. 3. The highest REC value of
63.45% was found in the thawing slices of group B, following
down by 58.62% in thawing slices of group D, 55.77% in
thawing slices of group A, 40.66% in thawing slices of group
C, 39.33% in the blanched slices (blanching) and 31.22% in
the raw fresh slices (control group). A significant difference
in REC was observed between any two groups except the
pair of blanching and thawing in group C, thawing in group
A and thawing in group D. It can be concluded from the REC
results that refrigerator freezing conducted more damage to
cell tissue than vacuum freezing, and combined treatments of
blanching and freezing conducted a further more damage on
the micro-structure of apple tissue than the single one.
2.5 Micro-structure analysis by light microscope
100 µm 100 µm
a b
100 µm 100 µm
c d
100 µm 100 µm
e f
a. control group; b. blanching group; c. thawing in group A;
d. thawing in group B; e. thawing in group C; f. thawing in group D.
Fig. 5 Photomicrographs of apple tissues after different treatments
Fig. 5 shows the photomicrographs of apple tissue under different treatment conditions, in which thin layers lined cells surface profile. It was observed from Fig. 5a that cells in untreated fresh apple were regular in shape, arranged in order and appeared plump with an apparent consistent cell wall structure. However, blanching treatment caused the loss of turgidity of the cells, appearing irregular in cell shape, distortion in tissue and disorderly in arrangement (Fig. 5b), which was
62 2016, Vol.37, No.23 食品科学 ※基础研究
verified by the apparent phenomenon that blanched apple slices presented relatively more soft in tissue and smaller in volume-size compared with untreated fresh ones.
Fig. 5c shows the cell tissue of vacuum-freeze-thawed apple slices with blanching pretreatment, in which a similar tissue morphology as Fig. 5b was observed except that a small quantity of cell walls were disrupted. The cell tissue of vacuum-freeze-thawed apple samples without blanching pretreatment was shown in Fig. 5e with fewer structural changes in contrast to Fig. 5c, even retaining many regular and plump cells similar to those in Fig. 5a.
Cells in refrigerator-freeze-thawed apple slices with (Fig. 5d) and without (Fig. 5f) blanching pretreatment appeared more distortion and disruption in cell walls compared to Fig. 5c and Fig. 5e. It is well known that ice crystal size is closely related to the freezing rate. Vacuum freezing were performed with a much faster freezing rate than refrigerator freezing, resulting in smaller and more uniform ice crystals in the extracellular and intracellular region. In contrast, larger ice crystals in apple tissue were formed during refrigerator freezing contributing to more cell disruption and morphological changes.
It can be concluded from Fig. 5 that vacuum freezing without blanching pretreatment (Fig. 5e) caused the smallest morphological changes and the lowest breakage to apple cell tissue, flowing increasingly by Fig. 5c, f and d. And the aforementioned difference of ML, thawing loss and relative electrical conductivity in different treatments groups could be attributed to the micro-structural changes at cellular level in the sample tissue.
In Fig.1, ML in group A was significantly higher than that in group C. It might be due to that blanching pretreatment on apple slices softened cells tissue, enhanced cells permeability and promoted more water evaporation or ML during vacuum freezing. And TL in Fig. 2 and relative electrical conductivity in Fig. 3 indirectly reflect the degree of structural changes in apple cells shown in Fig. 5. For example, vacuum-freeze-thawed apple samples without blanching pretreatment had the smallest TL value and REC value which was confirmed by the minimum structural changes in Fig. 5e. Refrigerator-freeze-thawed apple samples with blanching pretreatment had the highest TL value and REC value conforming to the highest degree of disruption in cells tissue.
And for the purpose of freezing preservation, the least micro-structure disruption in Fig. 5e (vacuum freezing
without blanching pretreatment) was desired and accepted in priority in order to achieve a good quality of frozen products. However, the micro-structure changes on apples tissue due to blanching treatment and freezing methods would also cause different drying properties if the frozen products were used for vacuum freeze drying[25-26], which needed further studies in the near future.
3 Conclusions
The vacuum freezing properties of blanching-treated
apple slices were investigated in this study by comparing
with the traditional refrigerator freezing. Results from this
work demonstrated that blanching pretreatment provided
significant increase in ML of apple slices during vacuum
freezing. In addition, more than half of the ML and most of
temperature reduction happened within the first 10 min of the
vacuum freezing period. The total process of vacuum freezing
can be divided into 3 stages: pressure-reducing and flashing
stage, ice formation stage and deep freezing stage. Based on
the analyses of thawing loss, relative electrical conductivity
and photomicrographs, it has been concluded that vacuum
freezing causes less micro-structural changes and disruption
to apple cells tissue and contributes to smaller TL and REC
value in contrast to refrigerator freezing, and that blanching
treatment before freezing conducts further damage to cell
micro-structure than non-blanching pretreatment.
References:
[1] PRETAMO G, PALOMARES L, SANZ P. Broccoli (Brasica oleracea) treated under pressure-shift freezing process[J]. European
Food Research and Technology, 2004, 219(6): 598-604. DOI:10.1007/
s00217-004-1022-2.
[2] PALACIOS I, GUILLAMON E, GARCIA-LAFUENTE A, et al.
Effects of freeze-drying treatment on the aromatic profile of tuber spp.
truffles[J]. Journal of Food Processing and Preservation, 2014, 38(3):
768-773. DOI:10.1111/jfpp.12028.
[3] BRONFENBRENER L, RABEEA M A. Kinetic approach to modeling
the freezing porous media: application to the food freezing[J].
Chemical Engineering and Processing: Process Intensification, 2015,
87: 110-123. DOI:10.1016/j.cep.2014.11.008.
[4] XANTHAKIS E, LE-BAIL A, RAMASWAMY H. Development of
an innovative microwave assisted food freezing process[J]. Innovative
Food Science & Emerging Technologies, 2014, 26: 176-181.
DOI:10.1016/j.ifset.2014.04.003.
[5] LIU E, HU X, LIU S. Theoretical simulation and experimental
study on effect of vacuum pre-cooling for postharvest leaf lettuce[J].
Research on Crops, 2014, 15(4): 443-449.
[6] SCHMIDT F C, LAURINDO J B. Alternative processing strategies
to reduce the weight loss of cooked chicken breast fillets subjected to
vacuum cooling[J]. Journal of Food Engineering, 2014, 128: 10-16.
DOI:10.1016/j.jfoodeng.2013.12.006.
※基础研究 食品科学 2016, Vol.37, No.23 63
[7] FENG C H, DRUMMOND L, ZHANG Z H, et al. Effects of
processing parameters on immersion vacuum cooling time and
physico-chemical properties of pork hams[J]. Meat science, 2013,
95(2): 425-432. DOI:10.1016/j.meatsci.2013.04.057.
[8] SUN D W, ZHENG L. Vacuum cooling technology for the agri-food
industry: past, present and future[J]. Journal of Food Engineering,
2006, 77(2): 203-214. DOI:10.1016/j.jfoodeng.2005.06.023.
[9] MCDONALD K, SUN D W. Vacuum cooling technology for the food
processing industry: a review[J]. Journal of Food Engineering, 2000,
45(2): 55-65. DOI:10.1016/S0260-8774(00)00041-8.
[10] COGNÉ C, NGUYEN P U, LANOISELLÉ J L, et al. Modeling
heat and mass transfer during vacuum freezing of puree droplet[J].
International Journal of Refrigeration, 2013, 36(4): 1319-1326.
DOI:10.1016/j.ijrefrig.2013.02.003.
[11] CHENG H P, LIN C T. The morphological visualization of the water in
vacuum cooling and freezing process[J]. Journal of Food Engineering,
2007, 78(2): 569-576. DOI:10.1016/j.jfoodeng.2005.10.025.
[12] WANG Haiou, HU Zhichao, TU Kang, et al. Application of vacuum-
cooling pretreatment to microwave freeze drying of carrot slices[J].
Transactions of the Chinese Society of Agricultural Engineering, 2011,
27(7): 358-363. DOI:10.3969/j.issn.1002-6819.2011.07.063.
[13] ZHANG Haifeng, BAI Jie. Vacuum cooling and freezing of mutton[J]. Meat Research, 2008(9): 62-65.
[14] JAISWAL A K, GUPTA S, ABU-GHANNAM N. Kinetic evaluation of colour, texture, polyphenols and antioxidant capacity of Irish York cabbage after blanching treatment[J]. Food Chemistry, 2012, 131(1): 63-72. DOI:10.1016/j.foodchem.2011.08.032.
[15] CANET W, ALVAREZ M D, LUNA P, et al. Blanching effects on chemistry, quality and structure of green beans (cv. Moncayo)[J]. European Food Research and Technology, 2005, 220(3/4): 421-430. DOI:10.1007/s00217-004-1051-x.
[16] WANG Y, ZHANG M, MUJUMDAR A S, et al. Effect of blanching on microwave freeze drying of stem lettuce cubes in a circular conduit drying chamber[J]. Journal of Food Engineering, 2012, 113(2): 177-185.DOI:10.1016/j.jfoodeng.2012.06.007.
[17] DOYMAZ I. Effect of citric acid and blanching pre-treatments on drying and rehydration of Amasya red apples[J]. Food and Bioproducts Processing, 2010, 88(2/3): 124-132. DOI:10.1016/j.fbp.2009.09.003.
[18] GONZÁLEZ-FÉSLER M, SALVATORI D, GÓMEZ P, et al. Convective air drying of apples as affected by blanching and calcium impregnation[J]. Journal of Food Engineering, 2008, 87(3): 323-332. DOI:10.1016/j.jfoodeng.2007.12.007.
[19] BEVERIDGE T, WEINTRAUB S E. Effect of blanching pretreatment on color and texture of apple slices at various water activities[J]. Food Research International, 1995, 28(1): 83-86. DOI:10.1016/0963-9969(95)93335-R.
[20] LEBOVKA N I, PRAPORSCIC I, VOROBIEV E. Effect of moderate thermal and pulsed electric field treatments on textural properties of carrots, potatoes and apples[J]. Innovative Food Science & Emerging Technologies, 2004, 5(1): 9-16. DOI:10.1016/j.ifset.2003.12.001.
[21] IGNAT A, MANZOCCO L, MAIFRENI M, et a l . Surface decontamination of fresh-cut apple by pulsed light: effects on structure, colour and sensory properties[J]. Postharvest Biology and Technology, 2014, 91: 122-127. DOI:10.1016/j.postharvbio.2014.01.005.
[22] SANJUAN N, CLEMENTE G, BON J, et al. The effect of blanching on the quality of dehydrated broccoli florets[J]. European Food Research and Technology, 2001, 213(6): 474-479. DOI:10.1007/s002170100401.
[23] LI B, SUN D W. Novel methods for rapid freezing and thawing of foods-a review[J]. Journal of Food Engineering, 2002, 54(3): 175-182. DOI:10.1016/S0260-8774(01)00209-6.
[24] BOONSUMREJ S, CHAIWANICHSIRI S, TANTRATIAN S, et al. Effects of freezing and thawing on the quality changes of tiger shrimp (Penaeus monodon) frozen by air-blast and cryogenic freezing[J]. Journal of Food Engineering, 2007, 80(1): 292-299. DOI:10.1016/j.jfoodeng.2006.04.059.
[25] KOCHS M, KORBER C H, HESCHEL I, et al. The influence of the freezing process on vapour transport during sublimation in vacuum-freeze-drying of macroscopic samples[J]. International Journal of Heat and Mass Transfer, 1993, 36(7): 1727-1738. DOI:10.1016/S0017-9310(05)80159-0.
[26] KRAMER M, SENNHENN B, LEE G. Freeze-drying using vacuum-induced surface freezing[J]. Journal of Pharmaceutical Sciences, 2002, 91(2): 433-443. DOI:10.1002/jps.10035.