vascular injury panel 2 (human) kits

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Page 1: Vascular Injury Panel 2 (human) Kits
Page 2: Vascular Injury Panel 2 (human) Kits

MSD Biomarker Assays

MESO SCALE DISCOVERY®

Page 3: Vascular Injury Panel 2 (human) Kits

Table of Contents

Contact Information MSD Customer Service

MSD Scientific Support

Page 4: Vascular Injury Panel 2 (human) Kits

Introduction

µ

Page 5: Vascular Injury Panel 2 (human) Kits
Page 6: Vascular Injury Panel 2 (human) Kits

Principle of the Assay

Page 7: Vascular Injury Panel 2 (human) Kits

Kit Components

Page 8: Vascular Injury Panel 2 (human) Kits
Page 9: Vascular Injury Panel 2 (human) Kits

Additional Materials and Equipment

Optional Materials and Equipment

Safety

Page 10: Vascular Injury Panel 2 (human) Kits

Best Practices and Technical Hints

Page 11: Vascular Injury Panel 2 (human) Kits

Reagent Preparation

Page 13: Vascular Injury Panel 2 (human) Kits
Page 14: Vascular Injury Panel 2 (human) Kits

Protocol

Page 15: Vascular Injury Panel 2 (human) Kits

Validation

Page 17: Vascular Injury Panel 2 (human) Kits

Analysis of Results

Typical Data

Vascular Injury Panel 2 (human)

1 10 100 1000 10000 100000 1000000

100

1000

10000

100000

1000000

10000000

SAA

CRP

VCAM-1

ICAM-1

Concentration (pg/mL)

Sig

nal

Page 18: Vascular Injury Panel 2 (human) Kits

Sensitivity

Page 19: Vascular Injury Panel 2 (human) Kits

Precision

Page 20: Vascular Injury Panel 2 (human) Kits

Dilution Linearity

% 𝑅𝑒𝑐𝑜𝑣𝑒𝑟𝑦 =𝑚𝑒𝑎𝑠𝑢𝑟𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛

𝑒𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛× 100

Page 21: Vascular Injury Panel 2 (human) Kits

Spike Recovery

% 𝑅𝑒𝑐𝑜𝑣𝑒𝑟𝑦 =𝑚𝑒𝑎𝑠𝑢𝑟𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛

𝑒𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛× 100

Specificity

% 𝑁𝑜𝑛𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐𝑖𝑡𝑦 =𝑛𝑜𝑛𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑠𝑖𝑔𝑛𝑎𝑙

𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑠𝑖𝑔𝑛𝑎𝑙× 100

Interference

Page 22: Vascular Injury Panel 2 (human) Kits

Stability

Calibration

Page 23: Vascular Injury Panel 2 (human) Kits

Tested Samples

Assay Components

Page 24: Vascular Injury Panel 2 (human) Kits

References

Page 25: Vascular Injury Panel 2 (human) Kits

Appendix A

10 100 1000 10000 100000 1000000100

1000

10000

100000

1000000

10000000

Wash

Remove Wash

SAA Concentration (pg/mL)

Sig

nal

1 10 100 1000 10000 100000100

1000

10000

100000

1000000

10000000

Wash

Remove Wash

CRP Concentration (pg/mL)

Sig

nal

10 100 1000 10000 100000 100000010

100

1000

10000

100000

1000000

10000000

Wash

Remove Wash

VCAM-1 Concentration (pg/mL)

Sig

nal

1 10 100 1000 10000 10000010

100

1000

10000

100000

1000000

10000000

Wash

Remove Wash

ICAM-1 Concentration (pg/mL)

Sig

nal

Page 26: Vascular Injury Panel 2 (human) Kits

Appendix B

10 100 1000 10000 100000 100000010

100

1000

10000

100000

1000000

10000000

Multiplex

Individual Assay

SAA Concentration (pg/mL)

Sig

nal

1 10 100 1000 10000 10000010

100

1000

10000

100000

1000000

10000000

Multiplex

Individual Assay

CRP Concentration (pg/mL)S

ignal

10 100 1000 10000 100000 100000010

100

1000

10000

100000

1000000

10000000

Multiplex

Individual Assay

VCAM-1 Concentration (pg/mL)

Sig

nal

1 10 100 1000 10000 10000010

100

1000

10000

100000

1000000

10000000

Multiplex

Individual Assay

ICAM-1 Concentration (pg/mL)

Sig

nal

Page 27: Vascular Injury Panel 2 (human) Kits

Summary Protocol

o

o

Page 28: Vascular Injury Panel 2 (human) Kits

Catalog Numbers

Page 29: Vascular Injury Panel 2 (human) Kits

Plate Diagram