venus - pure-process.compure-process.com/wp-content/documentacion/biotecno... · bacterial...
TRANSCRIPT
AUTOCLAVABLEPRESSURE CONTROLLED R&D FERMENTERS/BIOREACTORS
VENUS
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
The peculiarity that makes unique fermentation processes is the difficulty and complexity of scale-up.
Successful scale-up means a shortened cycle to full-scale production, competitive advantage, and cost savings.
Currently bench top autoclavable R&D fermenters on the market are largely if not exclusively working at non controlled
atmospheric pressure. That represent a substantial limitation in terms of Oxygen Transfer for R&D and particularly for future
scale up. Solaris has filled the gap with the launch of VENUS.
VENUS
2
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
WHY TO INVEST
IN THIS PRODUCT
Removable jacketbefore autoclaving:
Lighter & Handling
improved heat transfer
Pressure controlled
up to 2 bar
High oxygen
transfer
VENUS typical applications includes the following:
Education & Basic research
Scale-up and scale-down studies
Process development and optimization
VENUS can be used for:
Biopharmaceutical
Biofuels
Food industry
Bioremediation
Bioplastic
Cosmeceutical
Nutraceutical
3
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
Up to 24 units managed with one HMI with
innovative PARALLEL process control
LEONARDO: smart controller designed to
provide an high level of automated
management of the fermentation/cultivation
processes
Batch, Fed batch or continous processes
Remote control via PC, tablet and
smartphone for process management and
after sale assistance
23’’ (single unit) or
27’’ (multi system) multi touch HMI
Different gas mixing strategies with
up to 5 TMFC
4
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
VENUS
Fully removable and cleanable glass jacket for
an improved heat transfer during autoclaving
Powerful/ Accurate brushless motor, from 1 to 2000 RPM. Online absorbed Torques (Nm) and Power (W) measurements obtaining an indirect density indication of the culture broth
Compact and modular PCS
(350x350x350 mm)
N.4 assignable Watson Marlow pumps,
all speed controlled in entry level
Modbus Digital Hamilton sensors
Pressure controlled
up 2 bar
Easier scaling up
High oxygen trasfer
5
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
Why a digital sensor?
Modbus Hamilton sensors
Sensor lifetraceability
Reducingbackground noise
Hamilton sensors (including Cell Density) has been integrated into Solaris PCS and Leonardo software
giving the user the benefit of having a unique platform.
Fully compensated digital sensors, store and transmit all relevant sensor data, including calibration and
diagnostic information.
Optical density sensor (total and viable cell density)
The Incyte sensor enables real-time, online measurement of viable cells in solution. The measurement is not influenced by
changes in the media, microcarriers, dead cells or debris, and is designed for mammalian cell culture, yeast and high-density
bacterial fermentation. Online measurement of viable cells makes it possible to detect events and respond in real time without
sampling.
ON LINE MEASUREMENT OF VIABLE CELL DENSITY
- Increase yield and lower production costs
- Detect changes in cell physiology with frequency scanning
- Precisely control harvesting for continuous culturing
- Early detection of process deviations
HOW IT WORKS
The Incyte measurement principle is based on
capacitance. In an alternating electrical field, viable cells
behave like small capacitors. The charge from these
small capacitors is measured by the sensor and reported
as permittivity (capacitance per area).
VIABLE CELLS POLARIZED
DEAD CELLS HAVE DEMAGESMEMBRANES AND DO NOT POLARIZED
PLATINUM ELECTRODES
ELECTRIC FIELD6
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
VENUS
The Dencytee sensor performs online measurement of total cell density in solution. The sensor is based on optical density,
which measures the turbidity of the cell suspension. The measurement is made at NIR (near-infra red) wavelengths so it is
insensitive to changes in media color. All particles and molecules that scatter light at 880 nm will be detected, including living
and dead cells as well as cell debris. This measurement is effective after inoculation when cells are expanding quickly but
concentrations are low, making capacitance-based readings less reliable.
GAS MIXING
ON LINE MEASUREMENT OF TOTAL CELL DENSITY
- Simple online measurement of cell growth
- Reliable values during the growth phase
- Early detection of process deviations
HOW IT WORKS
The Dencytee sensor emits light through a 5 mm window onto a
light detector. Cells in suspension absorb and scatter light so less
light is read by the detector. To compensate,
the sensor increases the amount of light emitted by the light
source to maintain a constant reading at the detector. By reading
the amount of light that is increased at the light source,
the Dencytee sensor can measure solutions with high cell densities.
Various controller and hardware configurations enable aeration strategies using air, oxygen, nitrogen or a mixture of these to
enrich the air. The mass-flow controller allows the exact flow rate control of individual gases. The flexible aeration options
integrated in the fermenter/bioreactor permit a wide range of different application giving to this system a substantial versatility.
• Thermal Mass Flow Controller in entry model
• Gas mixing through TMFC and solenoid valves
or numbers of TMFC
• Automatic gas mixing algorithms
• Toro and sintered spargers
5 mm WINDOW
LIGHT SOURCE INCREASESINSTENSITY TO COMPENSATEFOR THE TURBIDITY OF THE CELL SOLUTION
LIGHTDETECTOR
7
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
• Sterile single use sampling system up
to 180 sterile sampling per batch.
• Needlefree connector is designed to
reduce the risk of contamination during
sampling.
• The sterile combination of a syringe (3-5-
10-30 ml) and a non return valve guarantee
the sterility after sampling until the next use
Technical specifications
Material VALOX resin (external) silicone (internal)
Autoclavable 121-133°C (up to 30 minutes)
Residual volume 0.04 mL
Flow rate 165 mL/minute
Flat
Split-septum
Straight fluid path
Minimal deadspace
S Cube
Additional parameters in modular external boxes
for future PCS upgrade including dCO2, Cell
Density, Weight, Peristaltic pumps, ect.
Solaris new modular product design strategy decreases
time to market and the number of unique parts in the
product architecture
increasing the number of product variants. The result is a
lean, flexible and smart PCS.
LEDA sterile sampling system
8
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
VENUS
Leonardo 2.0
USER-FRIENDLY SOFTWARE
The software is the user’s best friend in experimental design planning and performing
trial runs, as well as analyzing and optimizing media and parameters for cultivation.
The graphical user interface enables you to select the software functions intuitively.
Data extracted are compatible with Windows Excel. However, Solaris has developed
a platform where to easily and quickly manage cultivation data. This software is
included in the fermenter/bioreactor supply and can be installed on unlimited
number of client’s PC or laptop.
Do it parallel: smarter..fasterLeonardo can be used for process
development (i.e. time-saving ∙ parallel
fermentation approaches) Up to 24
indipendent fermentations/cultivations
can be carried out simultaneously.
Parallel synoptic. Parallel trends comparison between units, current and old batches.
Do it wireless!Increased mobility: users can roam
around lab or reaching office or home
without losing their connection with the
running batch.
9
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
Data sheetVessel
Solaris Code Venus 2.0
Total Volume (liters) 2,00
Ratio D/H 1:2,0
Min. Working Volume (liters) 0,33
Max. Working Volume (liters) 1,50
Max. temperature 65°C
Operating pressure < 2 bar
Headplate Ports (14)
Sensors lenght (mm)
pH 225
dO₂ 225
Dimensions for autoclave (with Condenser)
Height (mm) 505
Diameter (mm) 225
Design Borosilicate Glass Jacketed Vessel
Materials Borosilicate Glass and AISI 316 L
Stirring
Drive Brushless Motor, Direct Assembly , 1-2000 rpm (bacterial), 1-500 (cell cultures)
Power (PN) 266 W
Impellers Select from: Rushtons impellers, Marine Impellers, Pitched blade
Thermoregulation
Control PID Control - Accuracy 0,1 °C - Jacketed with electrical heaters
Gas Control & Gas Mixing
Sparger and overlay Gas Control TMFC
Gas Mixing (Air,CO2,O
2,N
2) 1TMFC (included in entry level) +4 solenoid valves or + n. of additional TMFC
Sparger type Select from: Toro type (ring), syntered microbubbling both provided with 0,2 µm filter
Exhaust Condenser and 0,2 µm filter
Controller
Master Control Module from 1 to 24 units - Dimensions Height: 350 mm Largeness: 350 mm Depth: 350 mm
HMI with Leonardo software 23’’ for single unit , 27’’ for multi system parallel
n.1 port, Gas Sparger Input
n.1 port, Gas overlay
n.1 port, Gas out/Condenser
n.1 port, Sampling system
Peristaltic Pumps
up to 4 Watson Marlow 114, speed 10 - 60 rpm, volumetric flow 0,5-51 ml/min, application assignable from software
n.1 port, Harvesting system
n.1 port, Temperature sensor
n.1 port, multiaddition (4) needle free connectors
n.5 ports, spares
n.1 port, single addition needle free connectors
n.1 port, agitation group
10
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
Controls Chiller• Optionally VENUS can be equipped with a
chiller for heat removal from your culture minimizing lab water usage
• Using this system you don’t need a water supply line in your lab
• Cost-effective cooling of fermenters
• Easy operation
• Refregerant level monitoring
Chiller data sheet
Working temperature range -10°C / +40°C
Temperature stability ±0.5
Power consumption 0.7 kW
Filling volume range 2-8 L
Cooling output at 20°C
measured with ethanol
0.25-0.60 kW
Cooling output at 10°C
measured with ethanol
0.20-0.50 kW
Cooling output at 0°C
measured with ethanol
0.15-0.36 kW
Pump pressure max. 0.35-1.30 bar
Pump flow max. 16-35 L/min.
Dimension (WxDxH) 200x350x465 mm
Cooling output at -10°C
measured with ethanol
0.09-0.15 kW
TemperatureSensor PT100
Accuracy 0,1 °C
Control system Measuring resident in Leonardo 2.0 software
Control range 0 - 70°C
pHSensor Digital Hamilton sensor
Sensitivity 57 to 59 mV/pH
Control system Measuring resident in Leonardo 2.0 software
Control range 0 - 14
Operation temperature 0 - 130°C
Pressure range 0 - 6 bar
dO2
Sensor Digital Optical Hamilton sensor
Accuracy ±0.05%-vol, 21±0.2%-vol, 50±0.5%-vol
Control system Measuring resident in Leonardo 2.0 software
Control range 0,05 - 300% air saturation
Operation temperature -10 - 130°C
Pressure range 0 - 12 bar
Actuator Cascade to RPM, Gas Control, feedings,ect
Redox (ORP)Sensor Digital Hamilton sensor
Sensitivity 57 to 59 mV/pH
Control system Measuring resident in Leonardo 2.0 software
Control range ±2000 mV
Operation temperature - 10 -130°C
Pressure range ≤ 6 bar
ConductivitySensor Digital Hamilton sensorAccuracy ±3% Control system Measuring resident in Leonardo 2.0 software
Control range 1 - 3000 µS/cm
Operation temperature 0 -130°C
Pressure range 0 - 20 bar
Antifoam/LevelSensor Solaris sensor
Control Measuring resident in Leonardo 2.0 softwareINT
EG
RA
TE
D IN
S C
UB
E
WeightSensor load cells
Accuracy ±0.2 g
Control Measuring resident in Leonardo 2.0 software
dCO2
Sensor Mettler Toledo sensor
Accuracy ±10% (pCO2 10-900 mbar) ≥ ±10%(pCO2 > 900 mbar))
Control system Measuring resident in Leonardo 2.0 software
Control range 0,00-200% saturation
Operation temperature -20.0-150°C
Cell densitySensor Hamilton-Fogale sensor
Accuracy Mammalian cells in suspension ±5·104 cells/ml - Fermentation ±0.05 g/l dry weight
Control system Measuring resident in Leonardo 2.0 software
Pressure range 0-3 bar (option 1) 0-10 bar (option 2)
Operation temperature 0-60°C (option 1 ) 0-80°C (option 2 ) (max. sterilization temperature 135°C)
EX
TE
RN
AL
MO
DU
LAR
BO
X
Peristaltic pumpsWM 114 10-60 rpm
Option 1 Total cell density based on turbidity(Two ranges: 10^5 to 10^8 mammalian cells/ml - 0.5 to 100 g/L dry weight)
Option 2 Viable cell density based on capacitance(Two ranges: 5x10^5 to 8x10^8 mammalian cells/ml - 5 to 200 g/L dry weight)
VENUS
11
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016
SOLARIS BIOTECHNOLOGY srlVia Bachelet, 58 - 46047 Porto MantovanoMantova - ItalyPhone: +39 0376 408760Fax: +39 0376 385108Email: [email protected]
www.solarisbiotech.com
Pure Process Latinoamerica SA de CV Distribuidor Exclusivo. www.pure-process.com [email protected]
Tel.: +52 (55) 6273-4046 / 6273-4047 / 2465-1016