Life Sciences

The 21st century hero in your laboratory

Drug Discovery Research Clinical Screening

VICTOR2™

MultiLabel Counter

One compact instrument, a who

VICTOR2 is available in a version to suityour specific needs. You can select optionssuch as Barcode Reader, reagent dispensers,and temperature control.

Despite its power and flexibility, VICTOR2 is an easy-to-use laboratorytool. It switches from one measuring mode to the next without any physical adjustment or time delay.

The Wallac VICTOR2 multilabel counter from PerkinElmer Life Sciences

is today’s most advanced detection platform for non-isotopic assays.

To accommodate the full range of assays your work demands, it includes

high quality optical systems designed for each of the individual measuring

technologies. In the same protocol, for example, you can define up to 10

different labels.

ole new technology platform

To meet today’s needs, VICTOR2 accepts all types of microtitrationplates with between 6 and 1536 wells. Clear and opaque (black orwhite) plates, filterplates and membranes, Petri dishes, slides, filters and Terasaki plates are all accepted.

Any non-standard configuration can be defined using the instru-ment’s Windows Workstation software. The plates can be loaded manually or using 20-plate free access stackers (40-plate stackers are also available).

You can also integrate VICTOR2 into your robotics system.To meet the future demands on your laboratory, 32-bit WindowsWorkstation software is designed to utilize the full capabilities ofWindows NT, Windows 95 and Windows 98.

With VICTOR2, more than 10 counting modesare available at any time

Fluorescence (top and bottom)

Time-resolved fluorescence

Fluorescence polarization

Dual-window time-resolved fluorescence

Luminescence (glow, flash or dual type)

Photometry

Special dual label measurements

Dual excitation and emission fluorescence

Scanning of the well area

Fast and slow kinetic measurements

Not only is VICTOR2 easy to use, it is also easy to learn to use.

It gives you the freedom to employ it’s different technologies,

without forcing you to spend time learning how to use different

instruments. Nor do you have to waste time telling the instrument

things it should know. Details of the plates, filters and labels you

use are all pre-installed on VICTOR2.

It’s so easy but an extremely powerful tool

To measure your assay with VICTOR2,you simply load the plate, select aprotocol, and start measurement.Although easy to use, VICTOR2 doesnot restrict your freedom. It is a flexi-ble system providing access to count-less advanced features, all of whichare made available through the usersoftware.

Allowing everyone more time for science

There is a VICTOR2 configuration package to support every type of laboratory. Where manual loading is too slow, you can choose 20-plate or 40-plate stacker towers to allow automatic loading and continuouscounting.

The instrument’s HELP function is part of the versatileWindows Workstation package. You can click on pictorial“hot spots” to obtain more information about any feature ofthe counter. There is also an extremely comprehensivesearch function that allows you rapid access to specificinformation.

Factory set protocols cover labels, plates and filters, andthere is a PROTOCOL EXPLORER for quick access and editing of protocols. With ready-prepared protocols there is no need for manual tuning. However there are limitlessopportunities to create your own application-specific protocols.

After measuring of your plate, VICTOR2 automatically trans-fers results to appropriate application software such asMicrosoft Excel or MultiCalc®. Or you can manage bothassay design and results analysis using the instrument’soptional WorkOut™ data analysis software.

WorkOut™ data analysis software -ideal for both

endpoint and kinetics assays

With endpoint and kinetics assays, PerkinElmer WorkOut soft-ware provides a flexible and easy to use tool for assay design andresults handling. It’s a 32-bit application for Windows NT/95/98,and includes powerful features such as context sensitive help,and the facility to work with any number of documents open atonce. There is a wide range of curve fitting methods, kineticsreduction methods, data export facilities and graphics options.You can visualize your results with special 3D viewing and othergraphics options.

With 32-bit WindowsWorkstation software

The automatic file output feature permits export ofresults in the format you specify.

You can visualize your results with the special 3Dviewing and other graphics options.

Similar, thoughtful VICTOR2 features add distinction to your work within the wholegamut of molecular biology applications, bind-ing studies, environmental and food testing, toxicology and drug screening.

In both slow and fast kinetic assay, VICTOR2

allows faithful reproduction of physical condi-tions. Delays and repeats can be specified byplate, row or well.

More application and reference information isavailable at www.perkinelmer.com/lifesciences.

Designed to accommodate developing needs ofits users, VICTOR2 provides numerous functionswhich can be supplied now or at any time inthe future.

Whatever your label, whatever yo

Cell biology, molecular biology, binding studies and much more

VICTOR2 is a complete platform for quantitative detection of

light-emitting, or light-absorbing markers. It is suitable for high-

sensitivity time-resolved fluorometry, fluorescence, flash or glow

luminescence, flash absorbance and photometry. It is the ideal

detector for use with dozens of labels in use today, as well as

those which will gain in importance in the future.

The most versatile counting solution for all types of cell-based

assay, VICTOR2 offers specialities such as scanning function, top

and bottom measurement, temperature control and dispensing

capabilities. All this makes VICTOR2 a true platform for cell

applications.

Factory customized filters cover the fullranges of wave-length used in fluorometric

and photometric applications.

our assay

Examples of VICTOR2

application areasAgglutination

Apoptosis

Bacterial adhesion

Bacterial identification

Blue fluorescent protein

Cell adhesion

Cell counting

Cell expression

Cell membrane integrity and lysis

Cell proliferation

Cell viability and cytotoxicity

Chemotaxis

Chlorophyll

Colorimetric assays

Cytokine analysis

Cytotoxicity

DNA hybridization

DNA quantification

ELISA/FELISA

Endocrine hormones

Enzyme activity

Enzymatic cleavage

Enzyme kinetics

Environmental toxins

FIA

Gene expression

Green fluorescent protein GFP

HTS

Homogeneous TRF (LANCE)

Immunoassays

Kinase activity

LANCE

Macrophage activity

Metabolic activity

MIC (min inhibitory conc)

Mitochondrial membrane potential

Multiprobe assays

NADH,NADPH

Na+/H+ exchange

NK cell activity

Nucleic acid quantification

Oxidative burst

Oxidation reactions

PCR product -quant&qual

pH indicators

Phagocytosis

Phospholipid assay

PKU

Protease activity

Protein assays quantification

Receptor binding studies

Reporter gene

Reverse transcriptase

RNA hybridization

RNA quantification

Signal transduction

SNP (Single Nucleotide Polymorphisms)

Steroid hormones

Toxicology

Uranium

Platform for DELFIA® assays

Time-resolved fluorometry is today’s fastest growing non-isotopic label technology

... and the only one allowing multilabel assays

Time-resolved fluorometry (TRF) is based on the unique fluorescence properties of lanthanide chelates. Because theirfluorescence is as much as 200,000 times longer than conventional fluorophores, measurement starts only after allnon-specific fluorescence has died down. And because the Stokes shifts are so wide, measurement takes place atclearly separated wavelengths. The great specificity of each lanthanide’s emission spectrum also means you can measure several different labels as part of the same TRF assay.

Please ask your PerkinElmer representative for more information about our DELFIA system based on time-resolvedfluorometry.

Protein phosphorylation is a basic mecha-nism for modification of protein functionin eukaryotic cells. Tyrosine kinases areinvolved in immune, endocrine and nervous system physiology and pathology.Tyrosine kinase function increases thelevel of phosphorylated tyrosine in manycellular proteins which leads to variousactivation processes.

DELFIA assays are separationassays built on coatedmicrotitration plates. DELFIAassays have high sensitivityand wide dynamic range.The enhancement step is aunique proprietary feature ofDELFIA technology and it isthe key to high sensitivity.

VICTOR2 enables fast multiwavelength assays inmicroplates

Isoplate Top BottomConstant voltage 8.2 6.2Stabilized energy 13.4 3.6

Black plate Top BottomConstant voltage 2.4 N.D.Stabilized energy 4.4 N.D.

Calcium response following addition of ATP to cultured cells:Chinese hamster ovary cells were challenged with the purinergic agonist ATP, introduced using the VICTOR2 injector. Dual wavelengthmeasurement alternating between 340 and 380 nm every 0.5 s provides a ratiometric index of intracellular Ca2+ activity, enabling realcomparison between different experiments, cell types etc. The fast filter changer capability of the VICTOR2 enables the use of severaldyes monitoring different cellular functions for each experiment.

A limitation of cell-based fluorescent assays in microplates today is the necessity of using single wavelength lightsources. In many cases much more information could be gathered through the use of ratiometric indicators or indeedmultiple probes in one experiment. For assay development, or secondary screening the information content of the assayis as valuable as absolute speed. All VICTOR2 counters are capable of pharmacological measurements on end-point typeinflux experiments such as NMDA receptors or voltage gated Ca2+ channels. Injector-equipped VICTOR2 models will dosub second resolution single wavelength dye pharmacology for GPCRs. VICTOR2 does multi wavelength and/or ratio-metric Ca2+ analysis with sub second time resolution and integral addition of effectors.

Improved below-reading optics in the VICTOR2

A new feature of the VICTOR2 is the option of a direct light guide for the fluorescence excitation. This is especially useful for fluorescence assays reading microplates from below. On the VICTOR2 you can now do assays needing extra sensitivity for this mode of epi-fluorescence such as the Aurora Biosciences ß-lactamase reporter gene assay system. Note: Improved below reading is now standard item in all VICTOR2s.

Detection limits (fmol/well) forfluorescein in 96-well plate with

the new below reading light guide.

The VICTOR2 family meets allyour research needs

Fluorescence Luminescence Absorbance TRF FP Stackers

VICTOR2™1420-032 Y Y O O n/a O

VICTOR2™1420-033 Y Y O O n/a Y

VICTOR2™1420-011 Y Y Y O n/a O

VICTOR2™1420-012 Y Y Y Y n/a O

VICTOR2™1420-014 Y Y Y O n/a Y

VICTOR2™1420-015 Y Y Y Y n/a Y

VICTOR2™1420-042 Y Y O n/a Y O

VICTOR2™1420-043 Y Y O n/a Y Y

P10507 © 2002 PerkinElmer Life Sciences Inc.

Worldwide Headquarters: PerkinElmer Life Sciences, Inc., 549 Albany Street, Boston, MA 02118-2512 USA (800) 551-2121European Headquarters: PerkinElmer Life Sciences, Inc., Imperiastraat 8, BE-1930 Zaventem Belgium

Technical Support: in Europe: techsupport.europe@perkinelmer.com in US and Rest of World: techsupport@perkinelmer.comBelgium: Tel: 0800 94 540 • France: Tel: 0800 90 77 62 • Netherlands: Tel: 0800 02 23 042 • Germany: Tel: 0800 1 81 00 32 • United Kingdom: Tel: 0800 89 60 46

Switzerland: Tel: 0800 55 50 27 • Italy: Tel: 800 79 03 10 • Sweden: Tel: 020 79 07 35 • Norway: Tel: 800 11 947 • Denmark: Tel: 80 88 3477 • Spain: Tel: 900 973 255

Microsoft, Windows and Windows NT are registered trademarks of Microsoft Corp. All other trademarks or registered trademarks are the property of PerkinElmer Life Sciences, Inc.

www.perkinelmer.com/lifesciences

Specifications and Ordering InformationPlates 6, 12, 24, 48, 96, 384, 864 and 1536-well microtitration plates(outer dimensions 86 mm ¥ 128 mm, height 14-25 mm).1536-well plates can be used with fluorescence and FP. Petri dishes,slides, filters, Terasaki plates or customized plate formats can bemeasured with adapters.

PerformanceFluorometry Fluorescein detection limit < 2 fmol / well TR-fluorometry Europium detection limit < 10 amol / wellEu-linearity 5 decades Photometry Meas. range @ 405 nm 0- 2 A

Accuracy @ 405 nm < 2 % or ± 0.01APrecision @ 405 nm < 0.5 % or ± 0.01A

Luminometry Lower limit of detection < total flux of 105 photons/s

Fluorescence Fluorescein 1 nMPolarization with 384-well plate < 5 mP Standard

deviation

Physical dimensions (basic model)Height 383 mm (15.5”) (510 mm (20”) with stackers) Width 485 mm (19”) Depth 590 mm (23.5”)Weight 49 kg (110 lb), with stackers 59.5 kg (134 lb)

Software The instrument program runs on a Windows 95, Windows 98 orWindows NT compatible computer, minimum 16 MB memory,equipped with CD-ROM, SuperVGA display with minimum resolution 800 x 600 pixels and 256 colours.

Ordering Information

1420-011 Manual VICTOR2 with fluorescence, luminescence and absorbance technologies

1420-012 Manual VICTOR2 with fluorescence, luminescence, absorbance and TRF technologies

1420-014 VICTOR2 with fluorescence, luminescenceand absorbance technologies, stackers and robotic loading system

1420-015 VICTOR2 with fluorescence, luminescence,absorbance and TRF technologies, stackers and robotic loading system

1420-032 Manual VICTOR2 with fluorescence andluminescence technologies

1420-033 VICTOR2 with fluorescence and and luminescence technologies, stackers and robotic loading system

1420-042 Manual VICTOR2 with fluorescence, fluorescence polarization and luminescence technologies

1420-043 VICTOR2 with fluorescence, fluorescence polarization and luminescence technologies, stackers and robotic loading system

Options

1420-130 Temperature control

1420-217/218 Load/unload 40-plate stackers

1420-221 Barcode reader

1420-251 1-channel injector

1420-252 2-channel injector

1420-253 3-channel injector

1420-254 4-channel injector

1420-261 Additional pump, position 2

1420-262 Additional pump, position 3

1420-263 Additional pump, position 4