vincent a. chiappinelli, ph.d. the ralph e. loewy professor
DESCRIPTION
Vincent A. Chiappinelli, Ph.D. The Ralph E. Loewy Professor Chair, Dept. of Pharmacology & Physiology Tel: 202-994-3541 [email protected]. FUNCTIONAL PROPERTIES OF NEURONAL NICOTINIC RECEPTORS. NIH Competitive Renewal – 21 st Year Starts Dec 2006. - PowerPoint PPT PresentationTRANSCRIPT
Vincent A. Chiappinelli, Ph.D.The Ralph E. Loewy ProfessorChair, Dept. of Pharmacology &
Physiology
Tel: [email protected]
Principal Investigator: Vince ChiappinelliCo-Principal Investigator: Jian-Zhong GuoCo-Principal Investigator: Eva SorensonConsultant: David PerryConsultant: Michael McIntosh (Univ. Utah)
FUNCTIONAL PROPERTIES OF NEURONAL NICOTINIC RECEPTORS
NIH Competitive Renewal – 21st Year Starts Dec 2006
MAIN GOAL: Combine electrophysiological and morphological methods to determine roles of nicotinic receptors (nAChRs) in synaptic transmission in the CNS during development
Patch-Clamp Recording from Visualized Nerve Cells in Living Brain Slices
Allows Real-Time Recording of Synaptic Responses
Electrical Stimulation of Cholinergic Fiber Tract:Postsynaptic nicotinic response in SPL neuron
In Presence of GABA and Glutamate
ELECTRICAL STIMULATION OF CHOLINERGIC INPUTS HERE TO RELEASE ACETYLCHOLINE, THE ENDOGENOUS TRANSMITTER THAT ACTIVATES NICOTINIC RECEPTORS
Nicotinic Receptor Antagonist DHE Blocks Excitatory Effect of Released Acetylcholine
ACh increases evoked glutamatergic currents by 3-fold through activation of presynaptic nicotinic receptors
Ventral Lateral Geniculate Neuron (LGNv)
Schematic diagram of selected circuitry within the ventral Lateral Geniculate Nucleus (LGNv)
Locations of nicotinic receptors are indicated by asterisks. Pharmacological tools allow identification of subtypes of nAChRs.
4*
6*
7**
A: Autoradiography of 6* nicotinic receptorsB and C: Confocal Microscopy showing Co-Localization of Synaptotagmin and 7 nicotinic receptor subunit
-Conotoxin MII autoradiography in rat brain: LGN and optic tract heavily labeled revealing 6* receptor locations
Synaptotagmin (a presynaptic protein) immunolabeling showing synaptic terminals (white spots) around an SPL neuron (asterisk)
Co-localization (arrows) of 7 nAChR subunit and synaptotagmin in processes of an LGNv neuron