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VISOCOLOR®

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Manual VISOCOLOR® reagent case for soil analysis

Manual VISOCOLOR® reagent case for soil analysis 03.14MN

Contents

1. VISOCOLOR® reagent case for soil analysis ……………………………………………………………… 24

1.1 Analysis options ……………………………………………………………………………………………………………………… 24

1.2 Content of the VISOCOLOR® reagent case for soil analysis (REF 931 601) ……………………………………………… 24

1.3 Content of the VISOCOLOR® reagent case for soil analysis with PF-3 (REF 934 220) ………………………………… 25

1.4 Working procedure ………………………………………………………………………………………………………………… 26

2. Soil analysis procedure ……………………………………………………………………………………… 27

2.1 Sampling ……………………………………………………………………………………………………………………………… 27

2.2 Weighing and determination of moisture content ……………………………………………………………………………… 27

2.3 Sieving the soil sample ……………………………………………………………………………………………………………… 27

2.4 Determination of the soil density ………………………………………………………………………………………………… 27

2.5 Preparation of soil extract A ……………………………………………………………………………………………………… 28

2.6 Determination of the pH value …………………………………………………………………………………………………… 28

2.7 Determination of nitrate and nitrite ……………………………………………………………………………………………… 29

2.8 Determination of ammonium ……………………………………………………………………………………………………… 29

2.9 Preparation of soil extract B ……………………………………………………………………………………………………… 29

2.10 Determination of phosphorus …………………………………………………………………………………………………… 30

2.11 Determination of potassium ……………………………………………………………………………………………………… 30

2.12 Determination of soil type (sedimentation analysis) ………………………………………………………………………… 31

3. Procedure for photometric soil analysis …………………………………………………………………… 31

3.1 Preparation of soil extract AF ……………………………………………………………………………………………………… 31

3.2 Determination of pH value ………………………………………………………………………………………………………… 31

3.3 Photometric determination of nitrate …………………………………………………………………………………………… 32

3.4 Photometric determination of nitrite ……………………………………………………………………………………………… 32

3.5 Photometric determination of ammonium ……………………………………………………………………………………… 32

3.6 Preparation of soil extract B ……………………………………………………………………………………………………… 33

3.7 Photometric determination of phosphorus ……………………………………………………………………………………… 33

3.8 Photometric determination of potassium ……………………………………………………………………………………… 34

4. Calculation and correction of results ……………………………………………………………………… 35

4.1 Correction of moisture content …………………………………………………………………………………………………… 35

4.2 Calculation referring to areas ……………………………………………………………………………………………………… 35

4.3 Conversion into different dimensions …………………………………………………………………………………………… 35


MNManual VISOCOLOR® reagent case for soil analysis 03.14

1. VISOCOLOR® reagent case for soil analysis

NANOCOLOR®

NANOCOLOR®

1.1 Analysis options

Extraction solutions

·

·

·

·

Individual parameters Analysis REF®

®

VISOCOLOR® ECO

VISOCOLOR® ECO

VISOCOLOR® ECO

VISOCOLOR® ECO

VISOCOLOR® HE

VISOCOLOR® HE

1.2 Content of the VISOCOLOR® reagent case for soil analy-

sis (REF 931 601)

VISOCOLOR®

5

9 9

10

20

19 19

18

30

258 8

26

29

16

3231 31

37 37 3836 36

33 34 35

39

22111213

6

2423

19

17

17

2

2827

1421

43

15

15

6

7

7

1



1.3 Content of the VISOCOLOR® reagent case for soil analy-

sis with PF-3 (REF 934 220)

VISOCOLOR®

5

9 910

30

8 826

11

13

5032 49

47 4745 46

42 43 44

18

22

6

5123

12

51 51 51

17

17

2

4140

14

2143

15 15

6

7

7

1

282739

No. Item REF

HE

HE

®

®

HE

HE

HE

HE

ECO

ECO

ECO

ECO

ECO

ECO

ECO

ECO

ECO

ECO

ECO

48



1.4 Working procedure

(see 2.6, page 28) (see 3.3, page 32)

(see 2.7, page 29)

NH

(see 3.5, page 32)

NH

(see 2.8, page 29)

(see 2.10, page 30)

(see 3.7, page 33)

(see 2.11, page 30)

(see 3.8, page 34)(see 4, page 35)



2. Soil analysis procedure

2.1 Sampling

(see 2.2, page 27, see

2.4, page 27)

2.2 Weighing and determination of moisture content

Determination of moisture content:

[ ] [ ]

[ ]

210 x 279 mm

50

100

150

200

250250 ml

pp

C

MAX

pp

135

MAX

AP

PR

OX

IMA

TE

VO

LU

ME

S

2.3 Sieving the soil sample

210 x 297 mm

2.4 Determination of the soil density

Procedure:

ml 100

90

80

70

60

50

40

30

20

10

Calculation:

[ ]



2.5 Preparation of soil extract A

Preparation of the extraction solution:

2

4

6

8

10ml

B.B

RA

UN

Neumann-Neander-Str. 6-8 · D-52355 Düren

MACHEREY-NAGEL

EXP

100 mL

CaCl2-VorratslösungCaCl2 stock solution

Concentré CaCl2Solución concentrada CaCl2


MACHEREY-NAGEL

1 L

Gebrauchslösung ACaCl2-Lösung

zur BodenuntersuchungExtraction solution A

CaCl2 solution

for soil determination

Solution d’extraction A Solution CaCl2 pour

la détermination de sol

Solución de extracción A Solución CaCl2 para

la determinación de suelo

®


MACHEREY-NAGEL

Destilliertes Wasser

Distilled water

Eau déstillée

Agua destilada

Preparation of the soil extract:

non-dried


MACHEREY-NAGEL

1 L

Gebrauchslösung ACaCl2-Lösung

zur BodenuntersuchungExtraction solution A

CaCl2 solution


Solution d’extraction A Solution CaCl2 pour


Solución de extracción A Solución CaCl2 para


50

100

150

200

250250 ml

pp

C

MAX

pp

135

MAX

AP

PR

OX

IMA

TE

VO

LU

ME

S

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MN 616 ¼ · Ø 185 mm

100 REF 532 018

ml 100

90

80

70

60

50

40

30

20

10

2

4

6

8

10ml

B.B

RA

UN

50

100

150

200

250250 ml

pp

C

MAX

pp

135

MAX

AP

PR

OX

IMA

TE

VO

LU

ME

S

2.6 Determination of the pH value

Procedure:

VISOCOLOR® HE

8.5

8.0

7.5

7.0

6.5

4.0

5.0

5.5

6.0

9.0

10.0

VISOCOLOR HE pH 4.0 – 10.0

Art.-Nr.

920 374

®

pH 4–104 x

HE


MACHEREY-NAGEL

®

pH 4–104 x

HE


MACHEREY-NAGEL



Note: For measurements with an electrometric pH meter, a

special soil extract A is prepared in the ratio 2 + 5, e.g. 20 g

soil + 50 mL extraction solution A. You may also use soil extract

AF (see 3.1, page 31).

5 mL

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2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

pH-Fix 2.0–9.0

100 farbfixierte Indikatorstäbchen für viele Anwendungen100 color-fixed indicator strips for multiple applications

auch geeignet für die pH-Bestimmung von Magensaft

also suitable for the pH determination of gastric juice

Normale Anwendung: Eintauchen – ablesen! Bei schwach ge-pufferten Lösungen Stäbchen längere Zeit (1–15 min) eintauchen.General use: Dip in – read off! If solution is weakly buffered leave test strip in solution for a longer time (1–15 min).

\EXP 2015-06LOT 18B116

REF

921 18

A011554 / Rev. 2012-04

4 °C

30 °C

2.7 Determination of nitrate and nitrite

®

0

1 mg/

5

10

20

40

80

®

0

10

25

50

100

250

500

Procedure:

Please note: Reclose the package tightly immediately after use.

Calculation of results:

2.8 Determination of ammonium

®

0

10 mg/L

25

50

100

200

400

®

5 mL

Neumann-Neander-Str. 6–8 · 52355 Düren

Germany · Tel.: +49 24 21 969-0

10 x

NH4+–1


Germany · Tel.: +49 24 21 969-0

10 x

NH4+–1

Procedure:

Close ammonium vial immediately after removing the test strip.


2.9 Preparation of soil extract B


B, the solution is to be discarded. Rinse the bottle several times

with hot water and prepare fresh solution.


MACHEREY-NAGEL

EXP

100 mL

CAL-VorratslösungCAL stock solution

Concentré CAL

Solución concentrada CAL


MACHEREY-NAGEL

1 L

Gebrauchslösung BCAL-Lösung

zur BodenuntersuchungExtraction solution B

CAL solution


Solution d’extraction B Solution CAL pour


Solución de extracción B Solución CAL para


®


MACHEREY-NAGEL

Destilliertes Wasser

Distilled water

Eau déstillée

Agua destilada




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MN 616 ¼ · Ø 185 mm

100 REF 532 018

ml 100

90

80

70

60

50

40

30

20

10

2.10 Determination of phosphorus

VISOCOLOR® ECO

Procedure:

®

P-16 x

HE


MACHEREY-NAGEL

®

P-26 x

HE


MACHEREY-NAGEL

®

P-K6 x

HE


MACHEREY-NAGEL

Phosphor in Boden / Phosphorus in soil

Phosphore dans le sol / Fósforo en suelo

REF

P

D 04721 / A020806 / 9

20 383 /

100

3

920 3831 - 20 mg P / 100 g

20

16

12

8

6

4

3

2

1

0

2.11 Determination of potassium

Procedure:


Germany · Tel.: +49 24 21 969-0

K-2 1 x


Germany · Tel.: +49 24 21 969-0

K-115 x


Germany · Tel.: +49 24 21 969-0

K-115 x 15

10

8

6

4

3

2

Ka

lium

/ P

ota

ssiu

m

m

g/L

K+

Ka

lium

/ P

ota

ssiu

m

m

g/L

K+

Ka

lium

/ P

ota

ssiu

m

m

g/L

K+

Ka

lium

/ P

ota

ssiu

m

m

g/L

K+

3 mg/L




Forest sedimentation analysis according to KRUEDENER

2.12 Determination of soil type (sedimentation analysis)

Procedure:

E

F

10 x

®


MACHEREY-NAGEL

PyrophosphatlösungPyrophosphate Solution

Solution de pyrophosphateSolución de pirofosfato

Example:

-

timation regulation [3] [6]

Mark Sand (%) Soil type

E

D

3. Procedure for photometric soil analysis

VISOCOLOR®

VISOCOLOR®

NANOCOLOR®

NANOCOLOR® NANOCOLOR®

3.1 Preparation of soil extract AF

see 2.5, page 28

Procedure:

3.2 Determination of pH value

Procedure:

VISOCOLOR® HE

NANOCOLOR®



3.3 Photometric determination of nitrate

Procedure with PF-3:

VISOCOLOR®

ECO

1

2

3

4

5ml

B.B

RA

UN


Germany · Tel.: +49 24 21 969-0

NO3-15 x


Germany · Tel.: +49 24 21 969-0

NO3-2 1 x

Preprogrammed submethods:

NANOCOLOR®


Procedure with further NANOCOLOR® photometers

NANOCOLOR®

NANOCOLOR®

Preprogrammed NANOCOLOR® photometers

NANOCOLOR® UV/VIS, VIS, 500 D, 400 D, 300 D, 250 D, PF-12

Other photometers

3.4 Photometric determination of nitrite

NANOCOLOR®

Preprogrammed photometers


Other photometers

3.5 Photometric determination of ammonium


VISOCOLOR®

ECO

NH

1

2

3

4

5ml

B.B

RA

UN


Germany · Tel.: +49 24 21 969-0

NH4-21 x


Germany · Tel.: +49 24 21 969-0

NH4-3 4 x


Germany · Tel.: +49 24 21 969-0

NH4-110 x


NANOCOLOR®





NANOCOLOR®



Other photometers

3.6 Preparation of soil extract B


B, the solution is to be discarded. Rinse the bottle several times

with hot water and prepare fresh solution.


3.7 Photometric determination of phosphorus


VISOCOLOR®

ECO

VISOCOLOR® ECO

1

2

3

4

5ml

B.B

RA

UN


Germany · Tel.: +49 24 21 969-0

PO4-16 x


Germany · Tel.: +49 24 21 969-0

PO4-2 6 x


NANOCOLOR®

Preprogrammed submethods




NANOCOLOR®



Other photometers

3.8 Photometric determination of potassium


VISOCOLOR® ECO

1

2

3

4

5ml

B.B

RA

UN


Germany · Tel.: +49 24 21 969-0

K-2


Germany · Tel.: +49 24 21 969-0

K-1 15 x 1 x


NANOCOLOR®

Preprogrammed submethods



Other photometers



4. Calculation and correction of results

4.1 Correction of moisture content

Calculation:

Factors for correction of soil moisture

CaCl2

A 1 + 1

(see 2.2, page 27)

CAL

B 1 + 20

(see 2.2, page 27)

CaCl2

AF 1 + 2

(see 2.2, page 27)

Example:

4.2 Calculation referring to areas

(see 2.1,

page 27)

Calculation: M x d x f x D x CF = R

M [ ]

d [ ]

f [ ]

D [ ]

CF [ ]

R [ ]

Example 1: Example 2:

4.3 Conversion into different dimensions

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