· web viewappendix 1. steps followed during preparation of permanent slides (slides a – m)...
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Appendix 1. Steps followed during preparation of permanent slides (Slides a – m)
Slide a. Collected parasites in petridish
Slide b. Relaxation of collected parasites in physiological saline for 1 hr
Slide c. Fixation of parasites in 10% formalin
Slide d . Washing of parasites in running water for 6 hrs
Slide e. Staining the parasites with Borax Carmine for overnight
Slide f. De-stain the parasites in acid alcohol until the stain removed
Slide g. Dehydration in 90% ethanol for 15 minutes
Slide h. Dehydration in 95% ethanol for 15 minutes
Slide i. Dehydration in absolute alcohol for 30 minutes
Slide j. Dehydration in acid alcohol for 30 minutes
Slide k. Cleared in Lectophenol for 15 minutes
Slide l. Mounted in Canada Balsam Slide m. Prepared permanent slides