welcome to the media cybernetics 2003 dealer conference silver spring, san diego, munich and...

Welcome to the Media Cybernetics 2003 Dealer Conference

Silver Spring, San Diego,

Munich and Singapore

August/September 2003

Confidential - Do not copy or distribute

Introducing Image-Pro Plus 5.0!

• Day One– 9:00AM Welcome & Opening Remarks– 10:00AM-Noon Image Pro Plus Five-0– Noon-1:00PM Lunch– 1:00-2:00PM Image Pro Plus Five-0 (Cont.)– 2:00-3:00PM SharpStack Five-0– 3:00-4:00PM 3D Constructor Five-0– 4:00-5:00PM Scope-Pro Five-0

• Day Two– 9:00-11:00AM What is IQbase?– 11:00-Noon How Can WE Demonstrate & Sell IQbase?– Noon-1:00PM Lunch– 1:00-2:00PM Business Meeting– 2:00PM Adjourn

Agenda for 2-Day Program

Image-Pro Plus 5.0More Answers on your Images!

Image-Pro PlusImage-Pro PlusFive-OFive-O

Image-Pro Plus 5.0

Some Highlights



• Alignment Correction

• Automatic Tiling and Stitching

• Tracking– Automatic and manual– Measurement Display Options

Exciting New Features


• Improved Stack Format Support– Handle stacks better and automatically

assign colors where applicable.

• Color Composite Improvements– Customize Dyes and Colors– Image Set and Existing Image Knowledge

• New Basic Capture Dialog

Introducing Image-Pro Plus 5.0!Exciting New Features



• New Processing and Display Tools– Despeckle Filter– L*A*B Color– Scale Bar– EDF Test Strips

– Local Zoom– Synchronized Playback of

Sequences– Synchronized Pan and Zoom– Surface Plot Overlay from EDF




• Snap to New Image– Allows users to automatically burn

measurement overlays onto an image

• Set Manager– Multidimensional viewer - includes aspects of

the improved sequence file support

• Spatial Calibration Improvements– List Management– System Calibration– Wizard




• Workspace Context Menu Additions– Local Zoom, dye tints, set capture AOI appear in

context menu

• Version Information– Helps tech support know which versions of Image-

Pro Plus and any plug-ins are running

• Default Settings Folder– Location where all settings and environment

information is stored for the product.




• Objective Information File– Important for those that work with microscopes;

links to Scope-Pro and AFA• Display Range/Contrast Enhancement

Interaction– User interface enhancement to make these

tools less independent• Dye Management

– Allows for custom dyes and for user to define color by emission wavelength

– Pre-Defined Dyes– Add New Dyes– Tint Commands




• New Graphical User Interface

• Toolbar Changes– Toolbar has added functionality

• New Menu Structure– Menu is more logical for new users.




• Very New! WorkFlow Manager – User defined function– Toolbar oriented

• Very New! User Identities– Login– Basic, Full, Biological, Industrial defaults


Image-Pro Plus 5.0

Key Selling points

Hands On


IPP 5.0 Alignment Correction

• Align images or a sequence in translation, rotation, and/or scaling.

• Potential applications would be alignment of:– images taken over time with movement– channels of multiple images from a beam

splitter– Serial sections– correcting for offset angle of a Greenough (off-

axis) stereo microscope


IPP 5.0 Alignment Correction


• Synchronized Playback of Sequences

• Synchronized Pan and Zoom

Other Tools

New Tools from the Solutions Zone


• EDF Test Strips and EDF Surface Plot

EDF Tools



EDF Tools



Automatic Tiling and Stitching

• Align and stitch images together using a Fourier transform edge detection algorithm based upon both pattern and contrast matching

• Options provide for the insertion of blank images, image order modification, and manual adjustment of pane location

• This will be useful for constructing panoramic views or for linking together multiple images taken with a motorized or non-motorized stage.


Automatic Tiling and Stitching


Tracking

• Manual Tracking– Click on objects frame by frame

• Automatic Tracking– Highlight objects of interest and let Image-Pro do the

heavy lifting

• Graphs generated automatically– Cumulative and point to point options

• Many Measurement Options– Distance, velocity, etc.


Tracking


NEW Acquire Dialog!

• New Basic Capture Dialog– Improved Nomenclature– Vertically aligned to conserve real estate– Most-used controls in one basic dialog

*AND*– AutoSet button – a one-button solution which:

• Resets camera• Performs Auto-White Balance (color only)• Calculates Auto-Exposure• Opens preview pane.


NEW Acquire Dialog!

Quickly assign and change settings

Lock Preview and Acquire Settings

AutoSet!


NEW Acquire Dialog!


Spatial Calibration Improvements

• System Calibration applied when a new image is captured in Image-Pro, Scope-Pro, or AFA

• New Calibration Wizard

• Automatic correction for binning in Scope-Pro


Spatial Calibration Improvements



• Color and orientation are easily chosen

Scale Bar


Local Zoom



Measurement Overlay

• Allows users to save measurement overlays burned onto an image

Press to snap measurements to image


Workspace Context Menu Additions- Right-click features

• Local Zoom• Dye Tints• Dye Management

– Allows for custom dyes and for user to define color by emission wavelength

– Pre-Defined Dyes– Add New Dyes– Tint Commands


Color Composite Improvements


– Customize Dyes and Colors

– Preset Dye List

– Links to Set and Image Information



Press to create new dye



Select color by visual inspection or emission wavelength!


Settings Improvements

• Default Settings Folder– All settings and environment

information is stored in this location

• Lens Information File– Linked to acquisition in Scope-Pro

and AFA


New Workflow Manager

User-Defined Workflow Toolbar– Use Existing Menu Icons– Create New Icons– Include Self-Contained Macros

without External Parameters


New Workflow Manager

Sharp Stack 5.0


Sharp Stack 5.0

• Improved Speed of calculations• Spherical Aberration correction• Included Algorithms

– No Neighbor– Nearest Neighbor– Inverse Filtering


Sharp Stack 5.0


Supplementary AutoQuant Modules

• SharpStack meets the majority market needs

• AutoDeblur Gold WF (widefield fluorescence)

– Contains blind (2D & 3D) deconvolution methods

• AutoDeblur Gold CF (confocal fluorescence)

– Contains confocal deconvolution methods• AutoDeblur Gold WCF (all of the above)

• AutoDeblur 2D (only single plane blind deconvolution)

3D-Constructor 5.0


3D-Constructor 5.0

• More Visualization effects• Manual Measurements• Automatic Measurements

– 3D Count/Size– Histogram object selection

• 4D Measurements • More Macros


3D-Pro Series Competition

• Very high prices• Small companies with mixed support

capability• Mixed distribution strategy• Poor dealer relationships• Poor integration into mainstream 2D

image analysis packages


3D-Pro Series3D Constructor: Volume Rendering


3D-ConstructorManual Measurements


3D-ConstructorAutomatic Volumetric Measurements


3D-ConstructorAutomatic Volumetric Measurements Over Time4D Tracking of objects!!

ScopePro 5.0


Scope-Pro 5.0 Improvements

• Improved minimal dialogs to save real estate

• Acquire up to 999 Z-stack images • X-Y stage initialization retained

between sessions• Calibration Improvements

– Light path and magnification changers modify calibrations

• Site map for user defined capture regions


Scope-Pro 5.0

AFA 4.5Advanced Fluorescence Acquisition


Advanced Fluorescence Acquisition (AFA) For customers who acquire complex image sets

• Multiple color channels (usually fluorescence) or microscope modes

• Time-lapse, motion studies• Stage position

– Multiwell plates– Area scans– User defined locations

• Multiple focal planes (Z-stack)


Advanced Fluorescence Acquisition (AFA) Enhanced Capabilities

Time-lapse

Multi-channel Multiwell plates

Z-stack Scan area

User-defined Stage Positions


Advanced Fluorescence Acquisition (AFA)Interface: First Use


Advanced Fluorescence Acquisition (AFA)Interface: Preview Tab


Scope-Pro Device Updates

• Check our website for device updates

Camera Kit Update


Camera Kit Products

• CoolSNAPcf• Evolution LC• Evolution VF (NEW!)• Evolution MP• Evolution QEi


CoolSNAP-Pro cf Kits

• 1392 X 1040 pixels• 12-bit chip, Cooled 5C below

ambient• Available in Bayer color or

monochrome or with CRI Micro*Color

• Faster readout speed (20Mhz.)• Preview at 10-12 frames/sec. -

full resolution mono• Significantly Improved

Sensitivity!• Reduced dark current noise

down to <2 electrons!• Supports longer integration for

fluorescence applications

Camera List - $4,999Dealer Discounts up to 36%!


Evolution LC™ Integrated Camera Kit

• Digital alternative to video microscopy

• Available in monochrome and color

• Features– Fully integrated with your

choice of Image-Pro software– FireWire (IEEE 1394)

C-mount camera– 1.3 megapixel array– Up to 14 fps at 1280 x 1024

res., 30 fps at 640 x 480– Laptop Kit


Evolution VF - cooled & uncooled version- mono & color

• Features– Designed for high resolution

brightfield scientific and industrial applications.

– A progressive scan interline CCD sensor gives a resolution of 1.4 million pixels in a 12-bit digital output.

– High-speed low noise electronics provide linear digital data

– Frame rates of up to 110 fps in region of interest and binning.

– The IEEE 1394 FireWire™ digital interface allows ease of use


Evolution VF - Specifications

CCD Sensor

Light Sensitive Pixels 1.4 million, 1392 x 1040

Binning Modes 2x2, 4x4, 8x8

ROI (Region Of Interest)

From 1x1 pixels up to full resolution, continuously variable

Exposure/Integration Control

12µs to 17.9min. in1µs increments

Sensor Type Sony ICX205 Progressive Scan Interline CCD, Monochrome, or Color

Pixel Size 4.65µm x 4.65µm

Linear Full Well 10,000 e-

Read Noise 12 e-

Cooling Available Yes

Cooling Type Peltier Thermoel. cooling 25 degrees Celsius below ambient

Digital Output 12-bit

Readout Frequency 20, 10, 5, 2.5MHz

Frame Rate 10fps full resolution @ 12-bits, 165fps maximum with binning and ROI


Evolution MP™ Integrated Color Camera Kit

• High resolution 5.5 megapixel color camera

• Features– Fully integrated with your

choice of Image-Pro software– FireWire (IEEE 1394)

C-mount camera– 3-5 fps at 2560x1920(30-bit);

25 fps at 640 x 480– Option for cooling – No interpolation or Pixel

shifting


Evolution MP™ Integrated Color Camera Kit

Imaging Chip

Type2/3” Sony ICX-282 2 to 1 interlaced

CCD color

Resolution 2588 x 1960


Readout Noise 9.8 electrons

Maximum Frame Rate 3-5 fps at 2560 x 1920 (36-bit)

Shutter Electronic shutter, no moving parts

Cooling TypeOptional Peltier thermoelectric

cooling to 10°C below ambient

Physical

Weight 710g

Lens mountC-mount, 2/3” optical format for

microscope or camera lens, plus a 1/4”-20 tripod mount

Interface

Operating System Windows® 98 SE, ME, 2000, XP

Data Format 10-bit

Interface Type IEEE 1394 FireWire

Maximum Data Rate 20 MHz at 8-bit and 10 MHz at 10-bit


Evolution QEi™ Integrated Camera Kit

• High Quantum Efficiency camera• Features

– Fully integrated with your choice of Image-Pro software

– FireWire (IEEE 1394) C-mount camera– 5 fps at 1360x1036(12-bit)10 fps at

1360x1036(8-bit)


Evolution QEi™ Integrated Camera Kit

Imaging Chip

Type2/3” Sony ICX-285 progressive scan

interline CCD monochrome

Resolution 1360 x 1036 Light Sensitive Pixels


Readout Noise 8 electrons

Dark Current 0.15 electrons/pixel/sec cooled

Maximum Frame Rate10 fps full resolution in 8-bit, 5fps in 12-

bit. Higher speed with binning and ROI functions (up to 4x4 binning)

Shutter Electronic shutter, no moving parts

Cooling TypePeltier thermoelectric cooling to 25°C

below ambient

Physical

Lens mount C-mount, 2/3” optical format

Interface

Operating System Windows® 98 SE, ME, 2000, XP

Data Format 12-bit

Interface Type IEEE 1394 FireWire

Maximum Data Rate 10 MHz and 20 MHz selectable at 12-bit

IQbaseExtracting Information From Images


Image-Pro IQbase

• Information and data management software for individuals and companies that need a flexible and powerful database solution


Image-Pro IQbase

• Object-Oriented Database– More powerful and faster searches than

relational databases found in most competitive products

– Compatible with SQL, Oracle, and other databases

– Can be fully integrated with the world’s leading scientific image analysis software, Image-Pro Plus, which contains numerous input options, measurement and analysis tools, and world-class support


Image-Pro IQbase - Versatile

• Data can be stored in a variety of locations

• Data is organized according to the users needs and desires

• Saves Time


Image-Pro IQbase - Versatile

• Can work with MS-SQL, Oracle and other database types

• Data can be made available to web servers for customers/collaborators to view over the world wide web


Image-Pro IQbase - Expandable

• Suitable for single-user, small workgroup, and enterprise-level deployment

• Seats are easily added• Database can handle virtually

unlimited entries


Image-Pro IQbase - Intelligent

• Automatically remembers user settings and preferences

• Understands and collects data from within Image-Pro Plus automatically

• Reports can be produced in a variety of formats (PDF, HTML, RTF, etc.)


Image-Pro IQbase - Programmable

• Includes Visual Basic for Applications®

• Create custom forms, tools and plug-ins from within the product


IQbase

• Top Features

1. Context Sensitive Searches2. Easy Addition of files to the database3. Image-Pro measurements savable and searchable4. Savable searches5. Multi-user login with optional passwords6. Administration7. Data display8. Report generator produces a variety of formats9. Customizable interface features10. Visual Basic for Applications11. Offline Storage


Who Will Buy IQbase?

• New Image-Pro customers (plug-in)– Archive measurements automatically

• Existing Image-Pro users– Network installations of IPP

• Near-line storage systems

• Researchers and organizations that need to improve their efficiency


IQbase

• Database product that includes programming, reporting, and image analysis features– Built for organizations that want to archive their

data and mine it for information at a later time– Perfect for organizations that need to

communicate their findings both internally and externally

– Ideal for those interested in combining powerful data management with image analysis

Future Product Discussions

Business Session


HEADQUARTERSMedia Cybernetics, Inc.8484 Georgia Avenue, Suite 200Silver Spring, MD 20910 USAPhone: + 1 301 495 3305Fax: + 1 301 495 5964Email: [email protected] Web: www.mediacy.com

welcome to the media cybernetics 2003 dealer conference silver spring, san diego, munich and...

Documents

pm image pro

noon image pro

pm scopepro

versions of image

environment information

pm lunch1

pm adjournagenda

pm sharpstack