what is going on in the lab?
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Teacher: Audrey Gonzalez CCISD Professor Mentor: Zhengdong Cheng, PhD Artie McFerrin Department of Chemical Engineering. What is going on in the lab?. What is going on in the lab?. What type of engineering is conducted in the lab? Chemical Engineering - PowerPoint PPT PresentationTRANSCRIPT
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Teacher: Audrey Gonzalez CCISD Professor Mentor: Zhengdong Cheng, PhDArtie McFerrin Department of Chemical Engineering
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What type of engineering is conducted in the lab? Chemical Engineering What is the research? Soft condensed matter: colloids, droplets, and cells
Liquid in liquid
Gas in liquid
Droplets
Cell
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(1) Colloidal Crystal Growth: Understanding colloid structure in order to Engineer Materials for Optical Computers
(2) Discotic colloids: Using colloids to make replications of disk like shapes like those in RBCs, TMV, and Histones
(3) Cell encapsulation: Creating protective “bubble” to encapsulate cells to release therapeutic substances for humans
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Colloidal crystals are 3 -D periodic structures formed from small particles suspended in solution
Band gaps can be created within the structure of crystal to create a “circuit” for light to travel through in order to engineer optical computers
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Crystals can be created by using a suspension of polymers (PNIPAM)
If there is a high volume fraction in suspension there are more crystals
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One method is to use the Belousov-Zhabotinsky reaction. This reaction is used to convert its chemical energy to mechanical
energy to order crystal structure.
Crystal with Grain Boundaries
Single Crystal
BZ reaction facilitated
annealing of colloidal crystals
How do you structure How do you structure colloidal crystals? colloidal crystals?
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Biosa G, Bastianoni S, Rustici MChemical waves CHEMISTRY-A EUROPEAN JOURNAL 12 (13): 3430-3437 APR 14 2006
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Disk shapes like those found in RBCs , TMV, Histones, and Lipoprotien E4 found in Alzheimers can be replicated to better understand their function
RBCs
Histones
Lipoprotein E4TobaccoMosaicVirus
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• Wax with 1% STADIS-450• Flow Rate : 4ml/h• Voltage: 2.8 kV
Tang, K. Q.; Gomez, A., J. Colloid Interface Sci. 1996, 184,500.
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Uniform wax emulsion Place wax emulsion in freezing temperature to form discs
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Encapsulating cells of interest with therapeutic agent with a “bubble-like” semi-permeable membrane
Implant cell capsules into humanCells release therapeutic substance
such as insulin for diabetics.
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Method: ElectrosprayMake “bubbles” of Alginic acid
solution with Turgitol (surfactant) then drop into a solution of Calcium Chloride
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One method is using MicrofluidicsCells are joined together with semi-permeable membrane, “bubble”.
Soft lithography (G.M. Whitesides, Harvard Univ.)
Encapsulation of bacteria for screening purposes. Small diameter agarose bead (20µm), with a single bacteria encapsulated into. http://www.nisco.ch/19.htm
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1. Using the Electrospray to create wax discs
Why? To replicate form of RBCs and study flow of disk
shaped objects 2. Make Agarose encapsulations and coat
using layer by layer coating to form a protective layer around encapsulation
Why?Coating is to create optimum mechanical strength that will allow for cell to survive in
vivo and at the same time be able to secrete essential hormones needed by body
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(1) Colloidal Crystal Growth: Making colloidal crystals for optical computers by creating ordered structure through BZ reaction
(2) Discotic colloids: Making colloidal disks via electrospray to study shape and flow of RBCs and Lipoproteins in Alzheimers disease
(3) Cell encapsulation: Encapsulating cells to release therapeutic substances for humans using electrospray and microfluidics
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Dr. Zhengdong ChengdongGrad student : Andres MejiaUndergrad student : Lucas KinardNSF