what’s new at npod? · 2013. 8. 5. · • dka • hla • fcp < 3.7ng/ml •
TRANSCRIPT
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What’s New at nPOD?
Annual Meeting Update 2012
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OPPC Update
Martha Campbell-Thompson, D.V.M, Ph.D., nPOD OPPC Core Director
• Please see poster
• (Dr. Irina Kusmartseva, Emily Montgomery)
• Advances in 2011 • Donors
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Case Processing Spleen
Head Body Tail
NonPLN
PLN
Lay slices to the right and place in cassette in same orientation (label left facing).
[PanUncinate region will be included in PanHead]
P1 O1 P2 O2
Pancreas: head, body, and tail- bread loaf so paraffin and OCT are harvested in alternating sections:
1. Snap frozen vials with minced tissues and with or without RNAlater- 4 or more vials each from junctions of head and body and body and tail regions.
2. Paraffin blocks- 5-10 (OCT takes precedence over fixed when sample size is small)
3. OCT blocks- 5-10 all regions or as indicated by size
PLN:
1. OCT blocks – 3-5- as many as feasible depending on total numbers
2. Cells- place several dissected LNs in 15ml tubes containing sterile RPMI, hold at refrigeration until shipped or processed. 1-4 tubes depending on numbers
3. Frozen vials as for pancreas. Paraffin is optional.
Spleen and NonPLN: as for PLN. Paraffin is optional.
Duodenal mucosa: as for pancreas. Paraffin is optional.
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Pancreas Reconstructions
Anatomical Orientation “as in vivo”
Blue- anterior Black- posterior
Superior
Inferior
Anterior Posterior AB
Campbell-Thompson et. al, J of Visual Experimentation, in press
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Expanded Phenotyping
*6020-02 PanTail
**NC
Case
PanTail
60XX
-04 P
anHead
60XX
-02 P
anHead
60XX
-04 PanB
ody
60XX
-02 PanB
ody
60XX
-04 PanTail
60XX
-02 PanTail
60XX
-01 Spleen
*6020-02 PanTail
**NC
Case
PanTail
60XX
-04 P
anHead
60XX
-02 P
anHead
60XX
-04 PanB
ody
60XX
-02 PanB
ody
60XX
-04 PanTail
60XX
-02 PanTail
60XX
-01 Spleen
Ki67+Insulin
CD3+Glucagon
Rb IgG+Ms IgG
Ms IgG+ G Pig IgG Spleen
60XX
-02 P
anHead
60XX
-04 P
anHead
Pancreatic Polypeptide
Campbell-Thompson et. al, J of Visual Experimentation, in press
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07
06
05
04
03
02
01 With-in blocks “catalogues” bar-coded serial sections slide scanner readable
Reconstructions
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Expanded in-house Analyses
• Pancreas RNA- snap frozen and/or OCT thick sections (Dr. Kusmartseva)
• Cell prep FC- before cryopreservation (Dr. Todd Brusko)
RIN 2.4 4.7 4.6 6.7
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Donors
0
10
20
30
40
50
60
70
2007 2008 2009 2010 2011 2012 (planned)
Don
or N
umbe
rs
Transplant
Other
T2D
T1D Medalist
T1D
Autoab Pos
No diabetes
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nPOD Case Classification
`In the absence of islet autoantibodies or histopathology can we determine whether an individual has Type 1a’ diabetes after death?
George Eisenbarth 2011
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For nPOD to impact the underlying mechanisms leading to T1D
……………………………….and ultimately complications
will require sufficient and appropriate cases early in disease process
• Antibody positive non-diabetic: multiple; younger • Early (new-onset) • Age matched controls: healthy; type 2 • Accurate phenotypic characterization: ideally before
death
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Challenge • Long duration of disease in many cases (median 12, range 1-44 years) • Incomplete information Information gathered from the terminal medical charts and OPO questionnaires; Type 1a vs Type 1b vs Type 2
• Exclude secondary causes eg steroids, medications, CFRD
• Exclude immediate potential co-morbidities which might impact subsequent findings
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<10 yrs >10 yrs
DAA- DAA+*
<30
DAA- DAA+*
Diabetes-associated autoantibodies
Age of Onset
BMI
≥30
DAA- DAA+*
T1D/T2D? MODY MODY Type 1 Type 1 Type 1 Type 2
* non-mIAA antibody
N=21 N=21 T1D N=0 T2D
N=47 N=31 T1D N=16 T2D
N=30 N=27 T1D N=3 T2D
N=17 N=4 T1D N=13 T2D
N=3 N=2 N=15
mIAA
N=2 N=2 T1D N=0 T2D
N=11 N=1 T1D N=10 T2D
N=4 N=1 T1D N=3 T2D
mIAA
N=15 N=14 T1D N=1 T2D
N=7 N=6 T1D N=1 T2D
mIAA N=7 N=6 T1D N=1 T2D
nPOD DIABETES CASES (n=68)
<2 ≥2
MODY screen
<2 ≥2
MODY screen
+ - + -
• DKA • HLA • FCP < 3.7ng/mL • <2 yrs to IDDM
<2 ≥2
• DKA • HLA • FCP < 3.7ng/mL • <2 yrs to IDDM
• DKA • HLA • FCP < 3.7ng/mL • <2 yrs to IDDM
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Current nPOD Data • Prior to Histology (n= 68)
52 Type 1 diabetes (13 1 Ab, 6 >1 Ab) 16 Type 2 diabetes (1 Ab)
• Histology (UF) Type 1 diabetes (52)
40 - no insulin+ islets 12 - insulin+ (8 reduced, 4 many, 3 amyloid, 3 C-peptide) 8 - insulitis
Type 2 diabetes (16) 1 - no insulin+ islets 15 - insulin+ (7 reduced, 8 many - 5 have amyloid, 5 C-peptide) 0 - insulitis
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Conclusion
Thorough phenotypic characterization suggests most cases classified correctly despite low frequency of islet autoAb in longstanding diabetes
Histology needed in islet autoAb negative
cases to distinguish type 1a vs type 1b
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16
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Antibody Screening Strategy Update
Clive Wasserfall, MS nPOD Autoantibody QA/QC Director
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!"#$%&'('%)*+,-% !% !./%01% !% 2345%6788
!" #" !
!"#$%&'#$"(&)*'&#++&"$%,-.&/'*01(&%#2"&/","'#++3&4"",&5".'"#(-,/6&#$&+"#($&(-,."&789:&;<=>&
?*@1#'"5&A-$%&'#$"(&)*'&<:::6&$%"&B-(1#,-.&1*10+#$-*,&"C1"'-",."5&$%"&+#'/"($&5".'"#("&-,&@*'$#+-$3&;<<>:&1"'.",$=6&A%-+"&$%"&,*,DB-(1#,-.&A%-$"&1*10+#$-*,&"C1"'-",."5&$%"&(@#++"($&@*'$#+-$3&5".+-,"&;7E>:&1"'.",$=&;76E=>
9*,%(:,*,%;+--,*,<=,>%?5%'@,%+<%(:,%*+>A%.-%;5+<@B
!"#$%&'#$"(&43&#/"&#'"&+*A"($&#@*,/&9D&$*&8D3"#'D*+5(&;F-/0'"&<=>&G,&<::86&H<>E&1"'.",$&*)&#++&5"#$%(&*..0''"5&#@*,/&$%*("&#/"5&I9&#,5&*2"'6&#,5&E:>7&1"'.",$&*..0''"5&#$&#/"&J9&#,5&*2"'>&
!"#$%&'()*+,-)&%,'.)/"&)01112),-')3%&4'.,)('5&'%.'.)+6)%4'7.$'5+85)('%,-)&%,'.)-%9')"55:&&'()#@*,/&$%*("&0,5"'&#/"&<:&*'&*2"'&#/"&98&;E6K=>
Figure 2. Age-specific death rates: United States, preliminary 2009
SOURCE: CDC/NCHS, National Vital Statistics System, Mortality.
<1 1–4
5–9
10–1
4
15–1
9
20–2
4
25–2
9
30–3
4
35–3
9
40–4
4
45–4
9
50–5
4
55–5
9
60–6
4
65–6
9
70–7
4
75–7
9
80–8
4
85+
Age (years)
Rat
e pe
r 100
,000
pop
ulat
ion
Age (years)
Rat
e pe
r 100
,000
pop
ulat
ion
<1 1–4 5–9 10–14 15–19 20–24 25–29 30–34 35–39
100
0
200
300
400
500
600
700
0
2,000
4,000
6,000
8,000
10,000
12,000
14,000
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Kronus Assay
• ELISA based on double antigen principle • No radiation • Fits into the capability of most screening
laboratories • Modified by nPOD to fit into a STAT
format.
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GADA and Age 20
GADA
0-10
11-20
50-60
60-70
71-80
0
15
30
45
n= 6153 34 51 29
p=NS Kruskal-Wallis
Age (years)
IU
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IA-2A and Age 21
IA-2A
0-10
11-20
50-60
60-70
71-80
0
20
40
60
80
100
120
140
n= 6153 34 51 29
p=NS Kruskal-Wallis
Age (years)
IU
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Screening Quality Control
Screened Kronus
Recovered Donor
Recovery Sample OPPC
Test Kronus
RIA Denver
Not Screened
Positive Or Negative
Screening Sample Clive
Retest Kronus
RIA Denver
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DASP
– GADA 93% Specificity, 86% Sensitivity – IA-2A 99% Specificity , 60% Sensitivity
• Raising cutoff to 18 GADA and 60 IA-2A – GADA 98% Specificity, 80% Sensitivity – IA-2A 100% Specificity, 58% Sensitivity
• Double positive with higher cutoff – 100% specificity, 54% sensitivity
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Moving Forward • Changed format of KRONUS kits to
double the number of screens / kit • Added ZnT8 to format • nPOD kit with simplified all in one kit • On call personnel routinely checking HLA
especially for single aab positive • Extra screens to focus on younger donors/
tissue donors.
24
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25
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Organ Procurement and Lab Relations
Jayne Moraski, MS nPOD Assistant Director
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Organ Procurement Partners Key:
• Dark blue = direct partners • Light blue = Screening labs • Green = Other Key Partners • Yellow = Shipping Partners • Purple = potential screening and tissue donor partners
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Laboratory Location Current Affiliated OPOs
Total Donor Estimate
Screening Start Date
MNIT (Los Angeles)
Golden State Lifesharing OneLegacy Intermountain CTDN (San Francisco)
Nevada Donor Network
18 70
240 42 150 20
October 2009 August 2009 November 2009 July 2010 March 2011 March 2011
LABS, Inc.(Philadelphia)
Gift of Life LifeChoice (CT) NJSharing Network New York ODN
180 30
100 150
October 2009 August 2011 September 2011 December 2011
LABS, Inc. (Denver) Donor Alliance 65 July 2007
Miami LAORA 30 August 2008
Total 1200 projected
2012 Aab Screening Projection Summary: Screened 778 donors in 2010 Screened 1,090 through October of 2011 – projected increase of 68%
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Goals
• Maintain relations and educational updates with labs and OPO partners
• Increase OPOs that screen by 25% for next year
• Identify new tissue donor programs
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30
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Finding New-Onset Cases: Expanding the Donor Pool
through Innovative Collaborations
Suzanne Ball, RN, MHS nPOD Director
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• 2,440,000 Total Deaths in U.S.
– 1,250,000 In-Hospital Deaths reported to OPOs
– 110,000 Potential Cornea Donors
– 50,000 Potential Tissue Donors
– 46,000 Cornea Donors recovered
– 25,000 Tissue Donors recovered
– 12,500 Potential Organ Donors (Medically Suitable)
• 7,994 Organ Donors recovered annually
• 70% consent for research 5600 Potential PA Donors
The Needle in a Haystack
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2012 Initiatives • Partner with Tissue Agencies to recover
asystolic donors outside the hospital • Collaborate with American College of
Emergency Physicians (ACEP) to identify and refer New Onset/DKA deaths in pediatric patients
• Pilot a project with an organ procurement organization (OPO) to develop teams for non-traditional donor recoveries
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Webinar/Case Discussions
Amy Wright, MS, MBA nPOD Investigator Relations
Coordinator
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• Vision: All nPOD investigators… – collaborate and move T1D research forward – make use of precious samples in the most
resourceful manner possible
• Purpose: Provide a forum for nPOD investigators to share and discuss data gathered on nPOD tissues.
Webinars/Case Discussions
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Webinars/Case Discussions
• Actions: Use web conferencing system to hold webinars to share images and engage in discussions – Follow-up with surveys/topic requests
• Case 6052 – Over 40 participants
• Viral Presence in nPOD Tissues – Formed workgroup with over 30 participants
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Webinars/Case Discussions
• Goals: – Provide a suitable forum for data sharing and
collaboration – Create focused workgroups to further discuss
and collectively answer questions – Choose topics that are relevant and garner
interest in forming workgroups
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JOHN KADDIS, Ph.D. City of Hope LES JEBSON, M.H.A. University of Florida
INFORMATION TECHNOLOGY
UPDATE
January 17, 2012
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• PHASE I – 90 Days – Information technology assessment – Data analysis – Hardware and software validation
• PHASE II – 120 Days – Prototype established – Assessment and testing on new platform – Identification of additional feature needs
• PHASE III – 30 Days – Address any training and orientation needs – Go-Live with new and improved Platform
Proposed Data Sharing Platform
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nPOD Data Flow: Now
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nPOD Data Flow: The Future
Image used w/permission, Adam Rauch, LabKey Software™
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Investigator Experience Survey
• Who was surveyed? 102
• Number who completed survey: 34 (33.3%), including 29 investigators (PI’s and Co-PI’s), and 5 post-docs or other staff
• Last Updated: 01/17/2012
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0
5
10
15
20
25
Strongly Disagree
Disagree Neutral Agree Strongly Agree
Process Simple
Requirements Clear
Data Entry Easy
Sample Availability Complete
New Investigator Application
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0
5
10
15
20
25
Strongly Disagree
Disagree Neutral Agree Strongly Agree
DB Access Easy Log-in Simple Easy Searching Sample Availability Clear
Online Pathology Database
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0
5
10
15
20
25
Strongly Disagree
Disagree Neutral Agree Strongly Agree
Sample Request Process Clear
Sample Limits Clear
Experiment Specific Needs Easily Conveyed
SRF Sufficiently Detailed
Timely Handling
Prompt Shipments
Service Request Forms (SRFs)
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0
5
10
15
20
25
Strongly Disagree
Disagree Neutral Agree Strongly Agree
Form Requirement Clear Form Easily Accessible Comments Easy to Share
Investigator Feedback Forms
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0
5
10
15
20
25
Strongly Disagree
Disagree Neutral Agree Strongly Agree
Streamlined Application, Tracking, and Feedback Choose level of data sharing Know who has viewed my data Real-Time Sample Availability
Future Online Data Sharing System
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Other Comments (Total Respondents) • How do you share data (9) : Discussions with colleagues/ publications/
meetings/ conferences/ nPOD webinars and meetings/ local lab
• Data sharing concerns (14): How will shared data be handled for similar projects/ Publishing novel findings, data repositories – when and how/ none – trust nPOD with unpublished information/ will this prevent collaboration and lessen rigor of study, promoting other labs to publish for the sake of being first/ sharing of stained sections submitted for publication but not yet accepted
• Desired security of nPOD online database (13): Restricted to nPOD data sharing investigators only/ restricted to registered members only/ high/ standard login/ protect data from being altered or corrupted/ disclaimer about unpublished data/ know who is accessing data
• Other Comments/Suggestions (8): Update online information for older cases where tissue pieces are no longer available/ advice on restricted samples that can no longer be accessed/ advance warning when a user reaches his or her limit to donor samples
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nPOD New Initiatives
• nPOD-Complications – Mark Atkinson
• nPOD-E – Carmen Retrum
• nPOD-T – Alberto Pugliese
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nPOD-c Mark A. Atkinson
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Genetic susceptibility
% Is
let C
ell M
ass
100
50
0
Inciting Event(s)
Chronic Disease
Time (years)
“Silent” β Cell Loss
Diabetes Onset
The Natural History of Type 1 Diabetes
• T2D • Animal Models • Lack of sample access (trials) • Failure to listen to patients • Complications heterogeneity • Etc., etc., etc.
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nPOD-C
“Proposed” nPOD-C aims at collecting and studying human tissues from donors with and without complications from T1D
Four areas of T1D complications:
• Cardiovascular disease • Nephropathy • Neuropathy • Retinopathy
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nPOD-C Group Members • Steering Committee:
– Martha Campbell-Thompson, Florida; Judy Hunt, JDRF; Stephen Rich, Virginia; Robert Levine, JDRF; John Malone, Southern Florida; Eva Feldman, Michigan; Tom Gardner, Penn State; Mike Mauer, Minnesota; Mike Steffes, Minnesota; Matthias Kretzler, Michigan; Dale Abel, Utah
• Cardiovascular Task Force: – Chair: Dale, Abel; Members: Ira Goldberg, Columbia; Dean Li, Utah; Christian Schulze,
Columbia; Heinrich Taegtmeyer, Texas; Renu Virmani, Maryland
• Nephropathy Task Force: – Co-chairs: Mike Mauer, Matthias Kretzler; Members: Hanna Abboud, Texas; Ron Tilton,
Texas; Erwin Bottinger, Mt. Sinai • Neuropathy Task Force:
– Chair: Eva Feldman; Members: Peter Nawroth, Heidelberg; Angelika Bierhaus, Heidelberg; Vera Bril, Toronto; Gordon Smith, Utah; Rayaz Malik, Manchester; Bill Kennedy, Minnesota; Jim Dyck, Mayo Clinic
• Retinopathy Task Force: – Chair: Tom Gardner; Members: Tim Kern, Case Western; Gerry Lutty, Johns Hopkins;
Hans Peter Hammes, Heidelberg; Victor Elner, Michigan; Ron Klein, Wisconsin; Peter Compochiaro, Johns Hopkins
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54
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The Future - Pushing the Boundaries and Advocating for Complications Research
in Type 1 Diabetes • Approach existing networks (complications, clinical trials) to
obtain more cases with well defined natural histories & medical records
• Obtain partial medical records of nPOD donors (in general population)
• Create data base for storage of: • PartialmMedical history • Research data
• Serve as a model for other registries/biorepositories in T1D…encouraging openness
• Include analysis of those with T2D
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56
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nPOD-E Europe
Carmen Retrum, M.S. nPOD Coordinator of Special
Projects
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Why is nPOD-E Important? • Main goals:
1. Expand organ collection (and distribution) by creating a network for organ recovery at qualified sites in European countries
2. Obtain immediate access to highly valuable stored samples
3. Provide the resources of nPOD tissues to European investigators, as well as those in the U.S.
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Why is nPOD-E Important? • Main goals:
1. Expand organ collection (and distribution) by creating a network for organ recovery at qualified sites in European countries
2. Obtain immediate access to highly valuable stored samples
3. Provide nPOD tissues and resources to European investigators, as well as those in the U.S.
4. Facilitate European distribution for perishable specimens
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nPOD-E Overview
• Collaboration sites in Sweden & Finland – Working together to increase nPOD
investigator access to tissue samples
• Individual sites established in Italy & Spain – Will screen, recover, store, and distribute – nPOD structural model utilized
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Collaboration Site Progress • Sweden:
– Collaborating with Dr. Gun Frisk, at Uppsala University, to obtain retrospective and prospective tissue samples, specifically islets from AAb+ donors.
– Have received 365 slides from a pre-existing donor collection.
• Finland: – nPOD-E is collaborating with Dr. Hyöty, at the University of
Tampere, and the PEVNET/Europod project by developing coordinated activities (specimen distribution, data, data management, other).
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Established 2 New Sites in Europe • nPOD-E Italy:
– Established new nPOD-E site in Siena, Italy with Dr. Francesco Dotta.
– Similar to nPOD, this project will screen and recover Aab+ and T1D donors from multiple sites and use all of nPOD’s SOP.
• nPOD-E Spain: – Established new nPOD-E site in Barcelona, Spain with Dr. Eduard
Montanya. – This project will recover from AAb+ and T1D donors. And use nPOD
SOP for its screening, recovery, case processing and distribution methods.
• Screening and recovery efforts for both to begin in February 2012.
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Case Recovery & Processing
(Per nPOD SOP)
Slides
To nPOD OPPC for immunopathology per SOP
and scanned to Aperio
Stored and managed
at nPOD-E Site
All other tissues
Inbound Processes
*Each site will manage their own case & specimen related data
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Investigator Request
TPC/OPPC review
nPOD-E Site
distributes tissue
Outbound Processes
*OPPC views tissue availability via database; site inputs distribution
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nPOD-E Projections
AAb Screens AAb Cases T1D Cases Total Cases
Spain 70-100 1-3 0-3 2-5
Italy 400+ 4-6 2-5 7-9
TOTAL 500 6 6 12 projected
Individual Site Expectations for 2012
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66
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nPOD-T Transplantation Alberto Pugliese, MD
nPOD Co-Executive Director
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The Type 1 Diabetes Spectrum
Time
Genetic Susceptibility/Protection & Environmental Factors
Autoimmune Responses
ß-cell Mass/Insulin Secretion diabetes
NO
RM
AL
RA
NG
E
Waves of regeneration? Survival of some ß-cells?
Autoimmune Reactivation,
Persistence and/or Waves?
INTERVENTION TRIAL TRANSPLANT PREVENTION
TRIAL
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0 50.0
0.1
0.2
0.3
0.4
0.5
0
1
2
3
5 6 7 8 9 10 11 12
C-peptide (ng/m
l)
0 50
10
20
30
40
50
60
70
0
1
2
3
4
5
6
7
8
9
5 6 7 8 9 10 11 12
GA
D A
Ab IA
2 A
Ab
IGR
P C
D8
T- C
ells
(%)
Euglycemia
Years post transplant
DM BIOPSY
C. PANCREAS TRANSPLANT BIOPSY
SPK Tx
4 1
A. AUTOANTIBODIES
Daclizumab - Thymoglobulin - Rituximab
2 3
B. C-PEPTIDE AND AUTOREACTIVE T CELLS
Patient 2 - SPK-3601
0.03 0.02 0.02 0.09
HLA
-A2
MP5
8 Pe
ntam
er-P
E
CD8-APC
HLA
-A2
IGR
P Pe
ntam
er-P
E 0.06 0.3 0.6
66 33 68 32
0.09
D. AUTOREACTIVE T CELLS
1
BLOOD
4
CD
3 IN
SULIN
C
D8
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INSULIN
GLUCAGON
VP-1
MERGED
Patient 2 (MSS), pancreas transplant biopsy
Left panel: a pancreatic islet stained for insulin (red), glucagon (light blue) and VP-1 (green).
Right panels: higher magnification of the inset from the left panel demonstrates colocalization of VP-1 and insulin. The image was selected from a Z-stack series acquired by confocal microscopy.
Vendrame et al. Diabetes 2010
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INSULIN CK-19 Ki-67
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years of follow-up
0 1 2 3 4 5 6 7 8 9 100
10
20
30
40
50
0
5
10
15
20
GAD Ab
IA2 Ab
ZnT8 Ab
IA-2 cutoff
GAD/ZnT8 Cutoff
GA
D a
nd Z
nT8
AA
bIA
-2 AA
b
SPK Tx
SPK IM-203 HLA A2, B8, DR3; A26, B72, DR7
HG
• Conversion of GAD & IA2 AAb ~4 years after Tx and conversion of ZnT8 AAb ~2 years later • The patient developed recurrent diabetes in July 2011 (about 9 years after Tx) • The patient was biopsied on 8/10/2011
Biopsy
unpublished
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SPK IM-203 HLA-A2 GAD CD8 T cells Direct Pentamer Staining (H Reijonen, unpublished)
A2‐GAD
pen
tamer
Memory
SSC
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The image cannot be displayed. Your computer may not have enough memory to open the image, or the image may have been corrupted. Restart your computer, and then open the file again. If the red x still appears, you may have to delete the image and then insert it again.
The image cannot be displayed. Your computer may not have enough memory to open the image, or the image may have been corrupted. Restart your computer, and then open the file again. If the red x still appears, you may have to delete the image and then insert it again.
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CD45
‐RA
SSC
1.16%
18.5%
0.9%
46.5%
39.1%
61.2%
PBMC
PLN
Pancreas
Positive in July, August (biopsy) and October 2011 GAD reactive, CD8 T cells in both naive and memory compartments; These cells were shown in blood, Tx PLN and pancreas transplant
unpublished
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SPK IM-203
BLOOD GLUCOSE (mg/dl) C-PEPTIDE (ng/ml) MMTT OGTT MMTT OGTT
• -10’ 192 198 1.28 1.05 • 0’ nd 196 1.31 0.99 • 30’ 265 273 2.71 1.08 • 60’ 280 410 2.99 1.17 • 90’ 302 453 2.63 1.19 • 120 294 477 2.33 1.27
MMTT 07/29/2011 HbA1c 10.7%
BIOPSY 08-10-2011
OGTT 10-4-2011 HbA1c 8.9%
INS CD8
unpublished
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nPOD-T nPOD-T aims at establishing feasibility of procuring human pancreatic tissue from transplanted T1D patients, when possible both transplanted and native pancreas
This should allow for scientific discovery in relation to:
• recurrent disease, which mimics spontaneous disease development, correlating biopsy data with clinical data
• immunosuppression and potential regeneration
• rejection and other chronic changes
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nPOD-T nPOD-T aims at collecting tissues in three different settings:
1. Organs/tissues from transplant recipients (post-mortem)
2. Biopsies of native and transplanted pancreas
• at the time of transplantation
• for recurrent disease and/or rejection, or hernia/other reasons
3. Archived biopsies
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TransplantCenters
Miami
Minneapolis
Indianapolis
nPOD-T - Miami Functions -Administration/coordination -Enrollment & Consenting (with Tx Centers) -Data management & information exchange with nPOD and Tx Centers -AAb testing Structure -PI (Pugliese) -Clinical Coordinator (IRB/consenting, medical records, archived specimen procurement, relations and nPOD and Tx Centers -Research Associate for AAb testing
nPOD - Gainesville Functions -Donor tissue procurement, archival storage, sample distribution to investigators -Histology -Data management Structure -PI (Campbell-Thompson) -Pathology Staff -Administrative Staff (Atkinson)
consent forms, IRB protocols, AAb results, coordination
nPOD Investigators
Specimens, data
Tissues from donors (via OPO), from biopsies, archived biopsy specimens
results, data, samples, study coordination interactions
Serum samples, medical data, documents
nPOD-T Organizational Diagram
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Conclusion
Thank you to all of you. Thank you JDRF. Questions?