Wheat leaf rust (Puccinia triticina) in Canada in 2006Wheat leaf rust (Puccinia

triticina) in Canada in 2006

Brent D. McCallum and Pat Seto-Goh, Cereal Research Centre, Agriculture and Agri-Food Canada

Wheat Leaf Rust 2006Wheat Leaf Rust 2006Wheat Leaf Rust 2006Wheat Leaf Rust 2006

• Light in eastern and central Manitoba Light in eastern and central Manitoba due to dry conditions but more severe due to dry conditions but more severe in western Manitoba and eastern in western Manitoba and eastern SaskatchewanSaskatchewan..

• Severity averaged 10.2% (flag leaf Severity averaged 10.2% (flag leaf coverage) in Manitoba and 5.3% in coverage) in Manitoba and 5.3% in SaskatchewanSaskatchewan

• Light in eastern and central Manitoba Light in eastern and central Manitoba due to dry conditions but more severe due to dry conditions but more severe in western Manitoba and eastern in western Manitoba and eastern SaskatchewanSaskatchewan..

• Severity averaged 10.2% (flag leaf Severity averaged 10.2% (flag leaf coverage) in Manitoba and 5.3% in coverage) in Manitoba and 5.3% in SaskatchewanSaskatchewan

Virulence AnalysisVirulence AnalysisVirulence AnalysisVirulence Analysis

• 317 isolates from across Canada 317 isolates from across Canada (MB+SK = 317, ON = 19, QC = 3, PEI = (MB+SK = 317, ON = 19, QC = 3, PEI = 16).16).

• 31 virulence phenotypes identified.31 virulence phenotypes identified.

• Most common were TDBG (50.1%), Most common were TDBG (50.1%), TDBJ (16.7%), MFDS (5.1%).TDBJ (16.7%), MFDS (5.1%).

• 317 isolates from across Canada 317 isolates from across Canada (MB+SK = 317, ON = 19, QC = 3, PEI = (MB+SK = 317, ON = 19, QC = 3, PEI = 16).16).

• 31 virulence phenotypes identified.31 virulence phenotypes identified.

• Most common were TDBG (50.1%), Most common were TDBG (50.1%), TDBJ (16.7%), MFDS (5.1%).TDBJ (16.7%), MFDS (5.1%).

SK+MB ON QC PEI CanadaRace # % # % # % # % # %TDBG* 9,16,26,3ka,11,17,30,B,14a,18 / 1,2a,2c,3,24,10 177 56 0 0 0 0 0 0 177 50.1TDBJ 9,16,26,3ka,11,17,30,B,18 / 1,2a,2c,3,24,10,14a 59 19 0 0 0 0 0 0 59 16.7MFDS 2a,2c,9,16,3ka,11,30,18 / 1,3,24,26,17,B,10,14a 0 0 14 74 3 100 1 6 18 5.1MFPS 2a,2c,9,16,11,18 / 1,3,24,26,3ka,17,30,B,10,14a 17 5 0 0 0 0 0 0 17 4.8TBBJ 9,16,24,26,3ka,11,17,30,B,18 / 1,2a,2c,3,10,14a 12 4 0 0 0 0 0 0 12 3.4MBPS 2a,2c,9,16,24,26,11,18 / 1,3,3ka,17,30,B,10,14a 11 4 0 0 0 0 0 0 11 3.1MDPS 2a,2c,9,16,26,11,18 / 1,3,24,3ka,17,30,B,10,14a 10 3 0 0 0 0 0 0 10 2.8TBBG* 9,16,24,26,3ka,11,17,30,B,14a,18 / 1,2a,2c,3,10 6 2 0 0 0 0 0 0 6 1.7MBDS 2a,2c,9,16,24,26,3ka,11,30,18 / 1,3,17,B,10,14a 3 1 0 0 0 0 2 13 5 1.4MDDS 2a,2c,9,16,26,3ka,11,30,18 / 1,3,24,17,B,10,14a 3 1 0 0 0 0 2 13 5 1.4MLDS 2a,2c,16,24,26,3ka,11,30,18 / 1,3,9,17,B,10,14a 5 2 0 0 0 0 0 0 5 1.4MCPR 2a,2c,9,16,24,11,14a / 1,3,26,3ka,17,30,B,10,18 0 0 0 0 0 0 4 25 4 1.1MBPR 2a,2c,9,16,24,26,11,14a / 1,3,3ka,17,30,B,10,18 0 0 0 0 0 0 3 19 3 0.8PBDG 2a,9,16,24,26,3ka,11,30,B,14a,18 / 1,2c,3,17,10 2 1 0 0 0 0 0 0 2 0.6TBDS 9,16,24,26,3ka,11,30,18 / 1,2a,2c,3,17,B,10,14a 0 0 2 11 0 0 0 0 2 0.6TNPJ 16,26,11,B,18 / 1,2a,2c,3,9,24,3ka,17,30,10,14a 2 1 0 0 0 0 0 0 2 0.6MBMR 2a,2c,9,16,24,26,11,17,14a / 1,3,3ka,30,B,10,18 0 0 1 5 0 0 0 0 1 0.3MBPG 2a,2c,9,16,24,26,11,B,14a,18 / 1,3,3ka,17,30,10 1 0 0 0 0 0 0 0 1 0.3MCGJ 2a,2c,9,16,24,3ka,17,30,B,18 / 1,3,26,11,10,14a 0 0 0 0 0 0 1 6 1 0.3MCPQ 2a,2c,9,16,24,11,14a,18 / 1,3,26,3ka,17,30,B,10 0 0 0 0 0 0 1 6 1 0.3MCQK 2a,2c,9,16,24,17,30,B / 1,3,26,3ka,11,10,14a,18 0 0 0 0 0 0 1 6 1 0.3MDBJ 2a,2c,9,16,26,3ka,11,17,30,B,18 / 1,3,24,10,14a 1 0 0 0 0 0 0 0 1 0.3MDNS 2a,2c,9,16,26,11,30,18 / 1,3,24,3ka,17,B,10,14a 0 0 1 5 0 0 0 0 1 0.3MJBJ 2a,2c,9,26,3ka,11,17,30,B,18 / 1,3,16,24,10,14a 1 0 0 0 0 0 0 0 1 0.3MMDS 2a,2c,16,24,3ka,11,30,18 / 1,3,9,26,17,B,10,14a 1 0 0 0 0 0 0 0 1 0.3TBDG 9,16,24,26,3ka,11,30,B,14a,18 / 1,2a,2c,3,17,10 1 0 0 0 0 0 0 0 1 0.3TBNS 9,16,24,26,11,30,18 / 1,2a,2c,3,3ka,17,B,10,14a 1 0 0 0 0 0 0 0 1 0.3TCSM 9,16,24,30,10,14a / 1,2a,2c,3,26,3ka,11,17,B,18 0 0 0 0 0 0 1 6 1 0.3TFPJ 9,16,11,B,18 / 1,2a,2c,3,24,26,3ka,17,30,10,14a 0 0 1 5 0 0 0 0 1 0.3TNBG* 16,26,3ka,11,17,30,B,14a,18 / 1,2a,2c,3,9,24,10 1 0 0 0 0 0 0 0 1 0.3TNBQ 16,26,3ka,11,17,30,14a,18 / 1,2a,2c,3,9,24,B,10 1 0 0 0 0 0 0 0 1 0.3Total 315 19 3 16 353* avirulent to LrCen

SK+MB ON QC PEI CanadaRace # % # % # % # % # %TDBG* 9,16,26,3ka,11,17,30,B,14a,18 / 1,2a,2c,3,24,10 177 56 0 0 0 0 0 0 177 50.1TDBJ 9,16,26,3ka,11,17,30,B,18 / 1,2a,2c,3,24,10,14a 59 19 0 0 0 0 0 0 59 16.7MFDS 2a,2c,9,16,3ka,11,30,18 / 1,3,24,26,17,B,10,14a 0 0 14 74 3 100 1 6 18 5.1MFPS 2a,2c,9,16,11,18 / 1,3,24,26,3ka,17,30,B,10,14a 17 5 0 0 0 0 0 0 17 4.8TBBJ 9,16,24,26,3ka,11,17,30,B,18 / 1,2a,2c,3,10,14a 12 4 0 0 0 0 0 0 12 3.4MBPS 2a,2c,9,16,24,26,11,18 / 1,3,3ka,17,30,B,10,14a 11 4 0 0 0 0 0 0 11 3.1MDPS 2a,2c,9,16,26,11,18 / 1,3,24,3ka,17,30,B,10,14a 10 3 0 0 0 0 0 0 10 2.8TBBG* 9,16,24,26,3ka,11,17,30,B,14a,18 / 1,2a,2c,3,10 6 2 0 0 0 0 0 0 6 1.7MBDS 2a,2c,9,16,24,26,3ka,11,30,18 / 1,3,17,B,10,14a 3 1 0 0 0 0 2 13 5 1.4MDDS 2a,2c,9,16,26,3ka,11,30,18 / 1,3,24,17,B,10,14a 3 1 0 0 0 0 2 13 5 1.4MLDS 2a,2c,16,24,26,3ka,11,30,18 / 1,3,9,17,B,10,14a 5 2 0 0 0 0 0 0 5 1.4MCPR 2a,2c,9,16,24,11,14a / 1,3,26,3ka,17,30,B,10,18 0 0 0 0 0 0 4 25 4 1.1MBPR 2a,2c,9,16,24,26,11,14a / 1,3,3ka,17,30,B,10,18 0 0 0 0 0 0 3 19 3 0.8PBDG 2a,9,16,24,26,3ka,11,30,B,14a,18 / 1,2c,3,17,10 2 1 0 0 0 0 0 0 2 0.6TBDS 9,16,24,26,3ka,11,30,18 / 1,2a,2c,3,17,B,10,14a 0 0 2 11 0 0 0 0 2 0.6TNPJ 16,26,11,B,18 / 1,2a,2c,3,9,24,3ka,17,30,10,14a 2 1 0 0 0 0 0 0 2 0.6MBMR 2a,2c,9,16,24,26,11,17,14a / 1,3,3ka,30,B,10,18 0 0 1 5 0 0 0 0 1 0.3MBPG 2a,2c,9,16,24,26,11,B,14a,18 / 1,3,3ka,17,30,10 1 0 0 0 0 0 0 0 1 0.3MCGJ 2a,2c,9,16,24,3ka,17,30,B,18 / 1,3,26,11,10,14a 0 0 0 0 0 0 1 6 1 0.3MCPQ 2a,2c,9,16,24,11,14a,18 / 1,3,26,3ka,17,30,B,10 0 0 0 0 0 0 1 6 1 0.3MCQK 2a,2c,9,16,24,17,30,B / 1,3,26,3ka,11,10,14a,18 0 0 0 0 0 0 1 6 1 0.3MDBJ 2a,2c,9,16,26,3ka,11,17,30,B,18 / 1,3,24,10,14a 1 0 0 0 0 0 0 0 1 0.3MDNS 2a,2c,9,16,26,11,30,18 / 1,3,24,3ka,17,B,10,14a 0 0 1 5 0 0 0 0 1 0.3MJBJ 2a,2c,9,26,3ka,11,17,30,B,18 / 1,3,16,24,10,14a 1 0 0 0 0 0 0 0 1 0.3MMDS 2a,2c,16,24,3ka,11,30,18 / 1,3,9,26,17,B,10,14a 1 0 0 0 0 0 0 0 1 0.3TBDG 9,16,24,26,3ka,11,30,B,14a,18 / 1,2a,2c,3,17,10 1 0 0 0 0 0 0 0 1 0.3TBNS 9,16,24,26,11,30,18 / 1,2a,2c,3,3ka,17,B,10,14a 1 0 0 0 0 0 0 0 1 0.3TCSM 9,16,24,30,10,14a / 1,2a,2c,3,26,3ka,11,17,B,18 0 0 0 0 0 0 1 6 1 0.3TFPJ 9,16,11,B,18 / 1,2a,2c,3,24,26,3ka,17,30,10,14a 0 0 1 5 0 0 0 0 1 0.3TNBG* 16,26,3ka,11,17,30,B,14a,18 / 1,2a,2c,3,9,24,10 1 0 0 0 0 0 0 0 1 0.3TNBQ 16,26,3ka,11,17,30,14a,18 / 1,2a,2c,3,9,24,B,10 1 0 0 0 0 0 0 0 1 0.3Total 315 19 3 16 353* avirulent to LrCen

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

2000 2001 2002 2003 2004 2005 2006

MBDS

TGBJ

THBJ

TBBJ

KBBJ

TBBG*

TDBG*

TDBJ

MFDS

others

Gene # % # % # % # % # %Lr1 315 100 19 100 3 100 16 100 353 100.0Lr2a 260 82.5 3 15.8 0 0 1 6.3 264 74.8Lr2c 262 83.2 3 15.8 0 0 1 6.3 266 75.4Lr3 315 100 19 100 3 100 16 100 353 100.0Lr9 10 3.2 0 0 0 0 0 0 10 2.8Lr16 1 0.3 0 0 0 0 0 0 1 0.3Lr24 272 86.3 16 84.2 3 100 3 18.8 294 83.3Lr26 18 5.7 15 78.9 3 100 9 56.3 45 12.7Lr3ka 42 13.3 3 15.8 0 0 10 62.5 55 15.6Lr11 0 0 0 0 0 0 3 18.8 3 0.8Lr17 57 18.1 18 94.7 3 100 14 87.5 92 26.1Lr30 41 13 2 10.5 0 0 8 50 51 14.4LrB 52 16.5 18 94.7 3 100 14 87.5 87 24.6Lr10 315 100 19 100 3 100 15 93.8 352 99.7Lr14a 126 40 18 94.7 3 100 7 43.8 154 43.6Lr18 0 0 1 5.3 0 0 9 56.3 10 2.8Total 315 19 3 16

CanadaSK+MB ON QC PEI

U of M Campus, winter wheatU of M Campus, winter wheatJune 20, 2006 June 20, 2006

U of M Campus, winter wheatU of M Campus, winter wheatJune 20, 2006 June 20, 2006

U of M Campus, winter wheatU of M Campus, winter wheatJune 20, 2006June 20, 2006

U of M Campus, winter wheatU of M Campus, winter wheatJune 20, 2006June 20, 2006

Saskatchewan, spring wheat, July 24+25 2006Saskatchewan, spring wheat, July 24+25 2006Saskatchewan, spring wheat, July 24+25 2006Saskatchewan, spring wheat, July 24+25 2006

Saskatchewan, spring wheat, July Saskatchewan, spring wheat, July 24+25 200624+25 2006

Saskatchewan, spring wheat, July Saskatchewan, spring wheat, July 24+25 200624+25 2006

Stripe rust teliospores

00050

50

25

1050

5 220

10

1 1 0 0 01

2 0

0

0

Severity of Stripe Rust in Saskatchewan July 24+25 2006

Leaf and Stripe Rust 2006Leaf and Stripe Rust 2006Leaf and Stripe Rust 2006Leaf and Stripe Rust 2006

• Low to moderate levels of leaf rust Low to moderate levels of leaf rust infection.infection.

• Dominant leaf rust virulence Dominant leaf rust virulence phenotype TDBG.phenotype TDBG.

• Stripe rust epidemic on spring wheat Stripe rust epidemic on spring wheat in central Saskatchewan and in in central Saskatchewan and in Alberta.Alberta.

• Low to moderate levels of leaf rust Low to moderate levels of leaf rust infection.infection.

• Dominant leaf rust virulence Dominant leaf rust virulence phenotype TDBG.phenotype TDBG.

• Stripe rust epidemic on spring wheat Stripe rust epidemic on spring wheat in central Saskatchewan and in in central Saskatchewan and in Alberta.Alberta.