where is the mutation?vorgogoz/articles/diapos... · 1.2 mb recombination mapping 50 kb reporter...
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![Page 1: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/1.jpg)
Where is the mutation?
D. mau.
D. sech.xxxxxx
x
xx
x
x
![Page 2: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/2.jpg)
Highresolution mapping of the cisregulatory mutation
D. sechellia
x
svb
selection of recombinants based on eye color
D. mauritiana
svbP[w+] P[w+]
1.2 Mb
recombination mapping
50 kb
reporter
enhancer bashing
AlistairMcGregor
![Page 3: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/3.jpg)
Among progeny individuals: select [orange eye] flies:
3/100 flies
![Page 4: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/4.jpg)
Highresolution mapping of the cisregulatory mutation
D. sechellia
x
D. mauritiana
svbP[w+] P[w+] svb
selection of recombinants based on eye color
1.2 Mb
50 kb
reporter
enhancer bashing
recombination mapping
One recombination event
every 2kb
![Page 5: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/5.jpg)
D. mauritianalike
D. sechellialike
D. sechellia
D. mauritiana
![Page 6: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/6.jpg)
D. mauritianalike
D. sechellialike
D. sechellia
D. mauritiana
Five types of recombinants
Three new phenotypes
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![Page 7: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/7.jpg)
Three enhancer regions
50 kb
reporter
D. melanogaster
reporter
D. sechellia
AlistairMcGregor
![Page 8: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/8.jpg)
Three enhancer regions
50 kb
reporter
D. sechellia
reporter
D. melanogaster
AlistairMcGregor
![Page 9: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/9.jpg)
D. sechelliax
D. mauritiana
svbP[w+] P[w+] svb
selection of 600 recombinants based on eye color
1.2 Mb
Where are the mutations responsible?
1
high recombination
![Page 10: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/10.jpg)
Among progeny individuals: select [orange eye] flies:
3/100 flies
![Page 11: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/11.jpg)
D. sechelliax
D. mauritiana
svbP[w+] P[w+] svb
selection of 600 recombinants based on eye color
1.2 Mb
What are the mutations responsible?
1
high recombination selection of 50 recombinants based on PCR markers
PCR marker
PCR marker
phenotyping
100 kb
2
3
svb
![Page 12: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/12.jpg)
One recombination event
every 2kb
D. sechelliax
D. mauritiana
svbP[w+] P[w+] svb
selection of 600 recombinants based on eye color
selection of 50 recombinants based on PCR markers
PCR marker
PCR marker
phenotyping
1.2 Mb
100 kb
What are the mutations responsible?
1
2
3
svb
![Page 13: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/13.jpg)
D. mauritianalike
D. sechellialike
D. sechellia
D. mauritiana
![Page 14: Where is the mutation?vorgogoz/articles/diapos... · 1.2 Mb recombination mapping 50 kb reporter enhancer bashing Alistair McGregor. Among progeny individuals: select [orange eye]](https://reader034.vdocuments.net/reader034/viewer/2022050216/5f61eda117b56274471fb9ec/html5/thumbnails/14.jpg)
D. mauritianalike
D. sechellialike
Five types of recombinants
Three new phenotypes
D. sechellia
D. mauritiana