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WHITE PAPER Temperature Module for Heating and Cooling Accuracy, Uniformity and Time-to-Temperature Written by Opentrons

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Page 1: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

WHITE PAPER

Temperature Module for Heating and Cooling Accuracy, Uniformity and Time-to-Temperature

Written byOpentrons

Page 2: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

2© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

The Opentrons Temperature Module is a hot and cold plate module that is able tomaintain steady state temperatures for use within the OT-2 liquid handler or as a standalone benchtop product. The Temperature Module can readily be used with the Opentrons App. Temperature capacity ranges from 4ºC to 95ºC.

The data in this paper illustrates the accuracy, uniformity and time-to-temperature of the module along with the 1.5 - 2mL and PCR thermal block accessories. All results were collected in real-world environments to better simulate conditions users are expected to experience in their own labs.

SECTION 1

Product Description

Temperature Module with PCR Thermal BlockTemperature Module with Flat Plate Adapter

Temperature Module with 1.5 - 2mL Thermal Block

Page 3: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

3© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

Accuracy: maximum measured difference between the LCD temperature display and the average measured thermal block temperature.

Uniformity : +/- temperature variation measured across the throughout the thermal block wells.

Time-to-Temp : total time for both the top plate and thermal block to reach the desired steady state temperature starting from ambient conditions.

Environmental Test Conditions: Data shown was collected at 22ºC and 37% relative humidity. Different test conditions may provide for different results.

1.5 - 2 mL Thermal Block

LCD Temperature Display

LCD DISPLAY TEMPERATURE (C)

TOP PLATE ACCURACY (C)

TOP PLATE UNIFORMITY (C)

TIME-TO-TEMP FROM AMBIENT (MINUTES)

4 ± 1 ± 0.3 18:00

37 ± 1 ± 0.15 3:00

65 ± 1 ± 0.3 6:00

SECTION 2

Temperature Module with 1.5 - 2 mL Thermal Block

Page 4: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

4© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

PCR Thermal Block

LCD Temperature Display

LCD DISPLAY TEMPERATURE (C)

TOP PLATE ACCURACY (C)

TOP PLATE UNIFORMITY (C)

TIME-TO-TEMP FROM AMBIENT (MINUTES)

4 ± 1 ± 0.3 12:00

37 ± 1 ± 0.15 3:00

65 ± 1 ± 0.3 6:00

SECTION 2

Temperature Module with PCR Thermal Block

Page 5: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

5© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

SECTION 3

Environmental Conditions and Condensation

It is recommended that laboratory conditions be < 22°C, < 60% relative humidity in order for the temperature module and its corresponding thermal blocks to reach the desired cold state temperatures near 4°C. Opentrons has not validated that the temperature module will function as desired within lab conditions of a higher temperature, humidity, or some combination of the two.

The operator may observe condensation on the module’s cold surfaces upon reaching values below room temperature. The exact temperature at which condensation will occur depends on the lab atmospheric temperature and relative humidity conditions. This can be calculated from any standard “Dew Point Index” condensation table.

WATER BATH PLASTIC TUBES, STRIPS AND PLATES

It is highly recommended that users place water between aluminum thermal blocks and plastic well tubes, strips and/or plates . Water baths allow for the highest temperature uniformity, accuracy, and time-to-temperature for the liquid inside the wells. Air gaps, container sizes/brands, and pressure applied to the container can all significantly affect temperature results without use of a water bath. The following tables provide suggested thermal block water bath volumes for adequate coverage of each well size.

TEST MEASUREMENT EQUIPMENT USED

Temperature Sensor: KS103J2 Thermistor

POWER USAGE

Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz

PCR THERMAL BLOCK0.2 µL STRIP OR PLATE

0.3 µL STRIP OR PLATE

Water Bath Volume 110 µL 60 µL

1.5 - 2 mL THERMAL BLOCK

1.5 mL TUBE 2.0 mL TUBE

Water Bath Volume 1.5 mL 1 mL

Page 6: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

6© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

128.00±0.13 194

85.75±0.13 89.75

84 80.1

127.75±0.13

85.50±0.13

DIMENSIONAL DRAWINGS

Temperature Module

Mass: 1.5kg

SECTION 4

Dimensional DrawingsMillimeter units are shown in all dimensional drawings.

Page 7: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

7© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

DIMENSIONAL DRAWINGS

1.5/2 mL Aluminum Thermal Block

Mass: 0.6kg

127.75

85.50

104.25

69.75

16.88

20.75

17.25 34.50

51.75

17.25 34.50

51.75 69.00

86.25

42.00

3.50

11.50

38.50

Page 8: WHITE PAPER Temperature Module for Heating and Cooling...Module input: 36V, 6.1A (220W max) Power adapter input: 100-240VAC, 50/60Hz PCR THERMAL BLOCK 0.2 µL STRIP OR PLATE 0.3 µL

8© OPENTRONS 2020 | TEMPERATURE MODULE GEN2

DIMENSIONAL DRAWINGS

PCR Aluminum Thermal Block

Mass: 0.4kg

11.25

14.38 9.00

18.00 27.00

36.00 45.00

54.00 63.00

72.00 81.00

90.00 99.00

127.75

111.00

85.50 75.00

9.00 18.00

27.00 36.00

45.00

54.00

63.00

18.50

17°

13.96

3.00 SECTION G-G

SCALE 5 : 6


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Fine Care Biosystems - ENDO...VARIABLE 0.1 - 2.5 µl 0.5 - 10 µl 2 - 20 µl FIXED 2.5, 5, 10 µl MUL TI 0.5 - 10 µl VARIABLE 5 - 50 µl FIXED 20, 25 µl MULTI 5 - 50 µl VARIABLE

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