why outsource to neurotar
TRANSCRIPT
Neurotar Ltd • www.neurotar.com
Why Outsource to Neurotar?
A Business Development Proposal from Neurotar Ltd
September 2010
Neurotar Ltd • www.neurotar.com
With Neurotar’s Value-Adding Services:
1. You save time and gain a unique competitive advantage Shorten your time-to-market and accelerate your lead optimization process by using Neurotar’s high-resolution, high-relevance in vivo two-photon microscopy services
2. You save money by avoiding costly preclinical mistakesBy providing content-rich data and comprehensive analysis, Neurotar helps you make better-informed preclinical decisions which boost your success in clinical trials
3. You by-pass the steep entry barrier Neurotar employs highly-skilled personnel with years of experience in sophisticated imaging technologies and a solid record in Neuroscience and Biophotonics
Neurotar Ltd • www.neurotar.com
AcCellerate: facilitates early, better informed preclinical decision-making
BeSure: improves predictive power and boosts success rate in clinical trials
CeeMore: enables simultaneous measurement of multiple parameters
DeeTails: visualizes fine changes in cell shape and function in real time
The ABCD of Neurotar’s services:
Neurotar’s place in drug discovery and development process:
Neurotar Ltd • www.neurotar.com
AcCellerate: Faster, better informed preclinical decisions
BeSure: Higher predictive power => Success in clinical trials
Lead search Lead validation in vivo
Ultimate lead validation in vivo Clinical trials
Clinical trials
time
Lead search
Lead validation in vitro
High-throughputscreening High Relevance
High Resolution
NEUROTAR’s in vivo microscopy
Successdue to
increased predictive
power
Failure due to
low predictive
power
High-throughputscreening High Relevance
Low ResolutionLow Relevance High Resolution
in vivo imaging
in vitro microscopy
“The advantage of multiphoton intravital microscopy <...> is the capability to chronically image structure, function and the therapeutic response at high resolution with superior contrast and maximal physiological relevance.”
Prof. Andrew Bullen, University of California San Francisco, USA
Neurotar Ltd • www.neurotar.com
DeeTails: high definition picture in vivo
Conventionallow-resolution in vivo imaging
NEUROTAR’S high-resolution in vivo microscopy
“Multiphoton microscopy <...> is the best high-resolution, noninvasive means of imaging in living animals <...> improving drug discovery and accelerating preclinical trials of clinical treatments.”
Prof. Sharron X. Lin, Cornell University, New York USA
Neurotar Ltd • www.neurotar.com
CeeMore: multiple parameters at once
Mono-parametric imaging (one channel)
NEUROTAR’s Multi-parametric imaging (2 channels or more)
“... In vivo multiphoton microscopy has revolutionized the way that biomedical researchers can study physiology, pathophysiology, and drug action.”
Prof. Kenneth W. Dunn, Indiana University Medical Center, USA
Neurotar Ltd • www.neurotar.com
Power of Two: CeeMoreDeeTails in vivo
Low-resolution Mono-parametric
High-resolution Mono-parametric
High-resolution Multi-parametric
Currently offered services: Neurotar’s advanced imaging services:
“Two-photon microscopy has opened a new era in the morphological and pathophysiological studies of the nervous system.”
Prof. Clément Ricard, INSERM, France
Neurotar Ltd • www.neurotar.com
Case study I: in vivo microscopy of A plaques in ADEnd-point histopathology versus Neurotar’s in vivo microscopy
“Multiphoton microscopy will continue to be a powerful tool in understanding and combating Alzheimer's disease.” Prof. Brian J. Bacskai, Massachusetts General Hospital, USA
6 mice for 4 months
Researchers’ time + animal use Σ 90 h
6 mice
Breeding & handling135 mice
for 2-4 months
Σ 300 hours135 mice
Resea
rche
rs’ ti
me
+ anim
al us
e
1 group = 15 miceTotal 9 groups
Histology(30h/group)Injections(3h/group)
1 group = 3 miceTotal 2 groups
in vivo imaging(6h/group)
Surgery + injections(6h/group)
Bonus: measurement of additional dynamic parameters accessible ONLY by NEUROTAR’s in vivo two-photon imaging (e.g., blood flow, Ca2+ signaling, transient BBB breach, mitochondrial membrane potential, cell motility etc).
Garcia-Alloza et al 2006 J Neuropath Exp Neurol
Vasculature, A plaques
and neuronalfine processesImaged in vivo
Amyloid plaques visualized in fixed post-mortem sections
Neurotar Ltd • www.neurotar.com
Case study II: in vivo microscopy in Stroke models
“At present, only 2-photon microscopy permits high-resolution, real-time imaging of structure and function within identified neurons in vivo.” Prof. Timothy H. Murphy, University of Alberta, Canada
Longitudinal quantification of fine structural changes after stroke:
Laser-induced thrombosis of cortical vessels (images by Neurotar): Vertical view:
50 m
Winship and Murphy 2009, Neuroscientist
Neurotar Ltd • www.neurotar.com
Case study III: in vivo microscopy in Migraine models
Serduc et al., 2006 Int. J. Radiation Oncology Biol. Phys.
In vivo two-photon images of BBB integrityand extravasation of fluorescent dyes
Before and After irradiation. Vertical view
In vivo two-photon images of reduced glutathione in meninges and cortex
Horyzontal view Vertical view
Sun et al., 2006 J. Biological Chemistry
Imaging Blood-Brain Barrier Imaging Redox Deregulation
“Functional two-photon microscopy has allowed the mechanisms of neurovascular coupling to be studied in vivo in unprecedented detail. <...> It enables the interrelations between individual neurons, glial cells, and vessels to be examined.”
Elisabeth M.C. Hillman, Columbia University, NY USA
Neurotar Ltd • www.neurotar.com
Technology of in vivo two-photon microscopy
Helmchen and Kleinfield, 2008 Methods in Enzymology
Astrocytic end-feet contacting arterioles
Multiparametric high-resolution imaging of glia and vasculature (images by Neurotar):
Crucial advantages of two-photon imaging:
- Deeper penetration into scattering tissue- Less phototoxicity and less dye bleaching - Ability to excite several dyes in one scan- Longitudinal studies (minutes to weeks)
0.01 mm
Neurotar Ltd • www.neurotar.com
Contact information:
Dr. Leonard Khiroug, CSO+358 45 635 2270
Skype: leokhiEmail: [email protected]
IN VIVO VERITAS“Life is not in a Petri dish, so why stare there?”