wizard genomic dna purification kit - promega/media/files/products and...wizard® genomic dna...
TRANSCRIPT
F eat u r es
Protocol
Scalable Purification
Purification Performance
Summary
reSourceS
Wizard® Genomic DNA Purification KitHigh Purity gDNA Yields Adjusted for Your Sample Volume
• Scalable purification protocols
• Stable gDna product
• fast recovery of gDna
• Pure gDna from a variety of species and sample types
used for blood sample types:
• low sample number, manual processing
• processing 50µl–10ml of blood
• r&D and clinical applications
W I Z a r D ® g e n o m I c D n a p u r I F I c at I o n k I t
featureS
p roto co l
Scalable Purification
Purification Performance
Summary
reSourceS
Process up to 24 samples in less than 1 hour with a 4-step precipitation protocol
Blood
Transfer supernatantto new tube containingisopropanol.
Centrifuge.
Aspirate ethanol.Air-dry pellet.Rehydrate DNA.
Discard supernatant.Add ethanol.
Centrifuge.
Centrifuge.
Discard supernatant.Vortex pellet. Add Nuclei Lysis Solution.Mix. Add ProteinPrecipitation Solution.
Centrifuge.
Add Cell LysisSolution. Incubate.
2818
MB
W I Z a r D ® g e n o m I c D n a p u r I F I c at I o n k I t
featureS
Protocol
s c a l a b l e p u r I F I c at I o n
Purification Performance
Summary
reSourceS
Scalable protocol for any sample sizeWizard® protocol can be adjusted for any sample volume.
• the Wizard® Genomic Purification Kit can process blood samples from 20µl up to 10ml
• Dna can be rehydrated overnight or for shorter time periods
comparison of Dna in Solution for Various rehydration conditions.b l o o D V o l u m e
100μl 300μl 1,000μl
rehydrate at 4°c overnight 3.1μg100%
8.5μg100%
35.5μg100%
1 part 10mm naoH for 10 minutes, then 3 parts rehydration buffer for 10 minutes at room temperature
87% 88% 81%
Water rehydration for 20 minutes at room temperature
42% 77% 50%
Dna rehydration buffer for 20 minutes at room temperature
77% 95% 53%
Values are given as percentages relative to rehydration at 4°c overnight.
W I Z a r D ® g e n o m I c D n a p u r I F I c at I o n k I t
featureS
Protocol
Scalable Purification
p u r I F I c at I o n p e r Fo r m a n c e
Summary
reSourceS 0
0.5
1
1.5
2
2.5
0
50
100
150
200
250
300
350
400
450
500
1#1 1#2 1#3 1#4 2#1 2#2 2#3 2#4
Ab
s r
ati
o
Yie
ld(
g)
Sample
Yield and purity of 10ml blood sample isolation
from two donors (n=4)
Yield
A260 /A280
A260 /A230
9363
MA
Efficient purification of gDNA from blood with the Wizard® Genomic DNA Purification Kit
10ml bood samples from two donors were processed using the Wizard® Genomic Dna Purification Kit. yield and purity were measured by absorbance spectroscopy.
W I Z a r D ® g e n o m I c D n a p u r I F I c at I o n k I t
featureS
Protocol
Scalable Purification
Purification Performance
s u m m a ry
reSourceS
High purity gDNA yields adjusted for your sample sizeBlood sample type:
• low sample number, manual processing
• processing 50µl–10ml of blood
• r&D and clinical applications
Wizard® Genomic DNA Purification Kit offers a traditional protocol that is:
• Fast & Simple: rapid isolation of gDna from blood in approximately 1 hour using a 4-step precipitation protocol
• Scalable: reagent volumes can be adjusted to your sample size for optimal gDna yields
• Flexibile: gDna purified from a variety of sample types (i.e., tissue culture, animal, plant cells, bacteria and yeast)
• Stable: Purified gDna stable for long-term storage
W I Z a r D ® g e n o m I c D n a p u r I F I c at I o n k I t
featureS
Protocol
Scalable Purification
Purification Performance
Summary
r es o u rc es
ResourcesProtocol: a1120, a1125, a1620
Applications: Processing different sample typesProcessing Samples in 96-well microplates
References: Pubmed citations list frequently asked Questions (faQs)
Ordering informationp r o D u c t s I Z e c ata l o g #
Wizard® Genomic Dna Purification Kit
100 isolations x 300µl a 1120
500 isolations x 300µl a 1125
100 isolations x 10ml a 1620
Kit Contents
• cell lysis Solution
• nuclei lysis Solution
• Protein Precipitation Solution*cat.# a1620 does not include rnase solution, option to purchase rnase a solution (4mg/ml), cat.# a7973, separately.
PromeGa corPoration • 2800 WooDS HolloW roaD • maDiSon, Wi 53711-5399 uSa • telePHone 608-274-4330 www.promega.com • ©2010 PromeGa corPoration • all riGHtS reSerVeD • PriceS anD SPecificationS Subject to cHanGe WitHout Prior notice • PrinteD in uSa 3/10 • 18910 • Part #iS004
• Dna rehydration Solution
• rnase Solution*