word of the day: enzyme: an enzyme is a substance that starts a chemical reaction in cells...
TRANSCRIPT
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Word of the Day:
Enzyme:An enzyme is a substance that starts a chemical reaction in cells
Enzyme Chemical acted upon
Product
lipase Fats and oils Glycerol and fatty acids
Protease Proteins Amino acids
Catalase Hydrogen peroxide
Water and oxygen
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What does your spit do to food?
Amylase Lab
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Background
• Amylase is an enzyme found in your saliva
• Amylase breaks down starches and turns them into sugars
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Remember your Latin and Greek
• Starch -------------> glucose + glucose + glucose +…
Amylase enzyme
Found in saliva
Substrate
Type of starch
Product
Type of sugar which your body uses for energy
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Let’s see how it really works…
• Step 1: Preparing the Lab– Get 10 test tubes– Label 1-10– Add 5 drops of
iodine to each test tube 1
2 dropper fulls of iodine
1
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Step 2: Prepare the saliva solution
• Collect 4 ml of saliva -– Spit through the straw into a
clean graduated cylinder– Add 17 ml of
water to graduated
cylinder and mix.
Use straw to collect saliva
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Step 3: Prepare the Starch Solution
• Using a 2nd graduated cylinder, get 7ml of starch solution and pour into a small beaker
Measure 7ml of starch solution into beaker
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Step 4:
• Mix the saliva solution into the starch solution
• Record the exact time of mixing
Saliva solution
Starch solution
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Step 5: Add Saliva solution to Test Tube #1
• Add 10 drops of saliva/starch to test tube # 1
• Record color: – Black/Purple: starch present– Yellow/Brown: no starch present
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Step 6: Repeat Step 5, Every Three Minutes
• Every three minutes, add 10 drops of saliva-starch solution to a new test tube– Record color
– Repeat until the starch is no longer present
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Data Table
Time Starch Present (Yes/No)
0
3
6
9
12
15
18
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Claim, Evidence and Explanation
• Write a CEE
• Due Next Class