Workpackage 0 (WP0): Management of project

Objectives: • Project management, supervision of sample

distribution and exchange of material between partners

• Confirmation of deliverables and milestones

• Submission of reports, supervision of periodic meetings and budget administration

• Supervision of websites formation and availability

Workpackage 0 (WP0): Management of project

Description of works:

1. Coordination of research activities by partners, flow of samples and data; interaction between partners

2. Supervision of two websites: a) Public with privileged access to partners

(contractor)b) Platform for data (P5)

3. Supervision of synthesis of genomic, proteomic and metabolomic data

4. Organization of semi-annual and annual meetings5. Integration and dissemination of results and maintaining

contacts with the EC (Dr. C. Krassnig)

4. Responsibility for all contractual activities, including intellectual properties

Workpackage 0 (WP0): Management of project

Deliverable titleDeliverable Number

Deliverable Date

Biennial and annual meetings

Short biennial reports and two periodic reports at M18 and M36

D1M6, M12, M18, M24, M30, M36

Formation of the public accessible project website

D2M12

Distribution of results and samples between partners

D3M36

Workpackage 1 (WP1): Mechanisms for establishment of dormant stage

• Objectives:

1. Characterize and optimize conditions for obtaining akinetes in culture (P1; Ora & Assaf)

2. Identification of genes, proteins and compounds associated with formation of akinetes in cyanobacteria and resting eggs in rotifers (P1, P5, P6: Ora, Assaf, Esther, Richard and Jens)

3. Studies on yeast sporulation and establishment of the dormant stage (P3, P5, P6: Stefan, Richard & Jens)

WP1: Description of work

1. The effect of environmental factors on akinetes formation (P1: Ora & Assaf)

2. Rotifer cultures and resting egg production (P1: Esther)

3. cDNA libraries and microarrays (Richard in samples supplied by Ora, Assaf & Esther)

4. Temporal expression of genes that are up- regulated or down-regulated during the formation of akinetes and resting eggs (Ora, Assaf, Esther & Richard)

5. Search for proteins and compounds (Richard and Jens on samples supplied by Assaf, Ora, Esther & Stefan)

6. Studies on sporulation and establishment of the dormant stage (Stefan)

Workpackage 1: Deliverables

DeliverablesDeliverable Number

Deliverable

Date

Optimal conditions for inducing of akinetes asexually and sexually reproducing rotifers and their resting eggs – Ora, Assaf & Esther

D4M9

cDNA libraries, EST sequencing and database construction, including a website accessible to Partners - Richard

D5M16

Pattern of global gene expression during formation of akinetes, rotifer resting eggs by microarray analysis – Ora, Assaf, Esther & Richard

D6M27

Quantitative analyses of the expression of specific genes by QPCR – Ora, Assaf & Esther

D7M35

D8M36

DeliverablesDeliverable

NumberDeliverable date

Proteins and metabolites identified to be associated with formation of resting eggs or contained within them – Richard, Jens, Ora, Assaf & Esther

D8M30

Genes, proteins and metabolites associated with the dormant stages of yeast spores – Stefan, Richard & Jens

D9M36

Workpackage 1: Deliverables (Continued)

Movement of samples - M6

• To Richard:

• 1) Ora, Assaf, Esther – samples for construction of libraries and

sequencing (to be discussed)

2) Stefan – Samples for Proteome analyses (?)

• To Jens: None

Workpackage 6 (WP6):Dissemination of results

Objectives and description of work: • Dissemination of results by participation in

scientific meetings

• The organization of a workshop on desiccation stress and dormancy

• Publication of a book on desiccation stress and dormancy

Workpackage 6 (WP6):Dissemination of results

DeliverablesDeliverable

NumberDeliverable date

Participation in scientific meetings D28M36

A workshop on dormancy: opportunities and challenges: preservation of cells and organisms

D29M24*

Publication of a book on dormancy D30M36*

* To be decided at the meeting