XXXIInd Winter School on

Proteinases and Their Inhibitors

Tiers am Rosengarten February 25 – March 1, 2015

We thank Shirley Knauer for her contribution to the cover picture. It shows the bright-field microscopy image of HeLa tumor cells overlaid with the respective fluorescence microscopy image of overexpressed exogenous human Taspase1 (colored in dark blue), located in nucleoli within the nucleus (stained in light blue). The colors of the bright-field image were altered. The right circular inset depicts the crystal structure surface of active Taspase1 (PDB ID: 2a8j) with its two subunits colored in blue and green. The active site (red patch) is targeted by a peptidyl-succinimidyl peptide (left circular insert), displayed as sticks with a semitransparent surface (orange). The picture thus illustrates our efforts to design a substrate analogue inhibitor of the putative cancer target Taspase1, a threonine protease that is frequently overexpressed in many different leukemias or Taspase1-dependent solid tumors.

32nd Winter School on Proteinases and Their Inhibitors in Tiers (Italy)

February 25 – March 1, 2015

Organizing Committee Klaudia Brix (Bremen) Christian Sommerhoff (Munich) Boris Turk (Ljubljana) Hans Brandstetter (Salzburg) Editors Michaela Lassacher & Hans Brandstetter Dept. Molecular Biology University of Salzburg Supported by

• Novo Nordisk • Stricker Laborhandel • University of Salzburg (Priority Program)

GENERAL INSTRUCTIONS

Accommodation

All Winterschool participants are accommodated in one of the three hotels Rose, Paradies or Vajolet, all of which are in five minutes walking distance. If you are unsure, please ask at the registration of Hotel Paradies. They have a list of all participants and are happy to help you.

While accommodation and meals including mineral water are covered, any extras are not:

• Please pay all drinks (except mineral water) directly after the meal. • Please do not forget to pay your good night-drinks before you leave for sleeping. • Please do not forget to pay all your extras (personal orders, …) at your hotel before

departure.

If you want to rent ski equipment, please ask at the Hotel Paradies.

Scientific sessions

All lectures will take place at the Haus der Dorfgemeinde (City Hall).

Social events

• Thursday evening, City Hall: Night session with the InhibiTiers Allstar Band • Friday evening, Paradies: Meet and Make Friends in Proteolysis Research • Saturday afternoon Several hiking tours, excursions • Saturday evening, Castle Prösels Gala Dinner & Farewell

Disclaimer

To enable and foster the unrestrained presentation and discussion of new results and ongoing research, every Winter School attendee must respect that all contents presented and discussed at the Winter School are private communications, not allowed for public use. Consequently, recording of lectures or photographing/copying of slides or presentations is prohibited.

PROGRAM

Regular talks are 8 min lecture plus 4 min discussion.

WEDNESDAY, FEBRUARY 25

18:00 Dinner

20:00 – 22:00 Wednesday evening session: Pathogen-derived proteases

20:00 – 20:05 Opening

20:05 – 20:15 Chairs: Esther SCHÖNAUER & Nathalie BEAUFORT: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

20:15 – 20:27 Carmen ABFALTER (University of Salzburg) The role of the serine protease HtrA in the pathogenicity of Gram-positive human gastrointestinal pathogens

20:27 – 20:39 Ewa BIELECKA (Jagiellonian University, Krakow) Cooperation between arginine specific proteases and PPAD from Porphyromonas gingivalis in generation of controlled state of inflammation in gums during infection

20:39 – 20:51 Przemyslaw GOLIK (Jagiellonian University, Krakow) Lit protease – an altruistic suicide factor in bacteria.

20:51 – 21:00 Katarzyna KUŚKA (Jagiellonian University, Krakow) Characterization of YaT cysteine protease from Caldanaerobacter subterraneus

21:00 – 21:12 Christine LEHMANN (MRC London) P. falciparum DPAP3 is a multifunctional cysteine protease involved in schizont segmentation and erythrocyte invasion.

21:12 – 21:24 Yesid A. RAMIREZ (University of Würzburg) Structural Secrets of ChlaDub1 from Chlamydia trachomatis

21:24 – 21:36 Marie-Theres VIELBERG (Technical University of Munich) Crystallographic analysis of the ClpP1/2 heterocomplex from Listeria monocytogenes

21:36 – 21:48 Rudolf WACHTEL (Technical University of Munich) Biochemical Characterization of the Rab Protease GtgE from Salmonella Typhimurium

21:48 – 22:00 Magdalena SCHACHERL (University of Cologne) Structural basis of proline-proline peptide bond specificity of a secreted protease implicated in motility of Clostridium difficile

THURSDAY, FEBRUARY 26

09:00 – 10:34 Thursday morning session I: Immunity & Signaling

09:00 – 09:10 Chairs: Linda TROEBERG & Brice KORKMAZ: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

09:10 – 09:22 Pernille SØGAARD (University of Oxford) DDR1 plays a role in spatial regulation of MT1-MMP activity during epithelial tubulogenesis

09:22 – 09:34 Neele SCHUMACHER (University of Kiel) ADAM-mediated proteolysis and IL-6 trans-signaling in acute inflammation

09:34 – 09:46 Kristin SCHUBERT (University of Oxford) LRP-1 mediated endocytosis of TIMP-3 controls inflammatory response in human macrophages

09:46 – 09:58 Haissi CUI (Technical University of Munich) Exosomes derived from a TIMP-1-conditioned, pre-metastatic liver are rich in Arginase-1 and sufficient to increase liver metastasis

09:58 – 10:10 Nežka KAVČIČ (Jožef Stefan Institute, Ljubljana) The effect of various antioxidants in cell death-related oxidative stress

10:10 – 10:22 Steffen RIETHMÜLLER (University of Kiel) Molecular analysis of the human Interleukin-6 receptor

10:22 – 10:34 Carla GUARINO (INSERM, Tours) Immune activation of neutrophils by antiPR3 mAbs is mediated by altered activity of membrane-bound proteinase 3

10:34 – 11:00 COFFEE BREAK

11:00 – 12:30 Thursday morning session II: Technology Meets Function

11:00 – 11:10 Chairs: Christian SOMMERHOFF & Martin RENATUS: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

11:10 – 11:22 Caroline HOPPE (Jacobs University Bremen) Visualization of tissue kallikrein 7 in human keratinocytes

11:22 – 11:34 Eileen KROLL (Jacobs University Bremen) Visualization of kallikrein 2 and 3 upon TSH stimulation in thyroid epithelial cells

11:34 – 11:46 Anna KEPPNER (University of Lausanne) The channel activating protease CAP2/Tmprss4 is not required for ENaC-mediated sodium homeostasis in vivo.

11:46 – 11:58 Maresa GRUNDHUBER (Ludwig-Maximilians-University of Munich) Generation of proteins and antibodies to study human mast cell tryptases

11:58 – 12:10 Aleksandra PECAK (Jagellonian University Krakow) Production of tissue kallikrein family proteases in novel expression system LEXSY

12:10 – 12:20 Han FANG (University of Salzburg) Human blood coagulation Factor VIII A2 domain expression, purification and interaction studies

12:45 LUNCH (Paradies, Rose)

THURSDAY AFTERNOON SESSIONS: CANCER BIOLOGY

14:30 – 15:46 Thursday afternoon session I: Cancer biology

14:30 – 14:40 Chairs: Thomas REINHECKEL & Christoph PETERS: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

14:40 – 14:52 Stefanie SCHMIDT (University of Kiel) Role of ADAM17 in the development of colon cancer

14:52 – 15:04 Jeanette SCHWARZ (University of Kiel) Polo-like kinase 2: A novel Regulator of ADAM17 Activity.

15:04 – 15:19 Dirk SCHMIDT-ARRAS (University of Kiel) A disintegrin and metalloprotease (ADAM) 17 in the metastatic niche is crucial for metastasis to the lung.

15:19 – 15:34 Björn RABE (University of Kiel) Senescence-associated release of transmembrane proteins involves proteolytic processing by ADAM17 and microvesicle shedding

15:34 – 15:46 Barbara GRÜNWALD (Technische Universität München) Hepatic Stellate Cells (HSCs) activated by TIMP-1/CD63/PI3K signalling express increased levels of metastasis-promoting genes.

15:46 – 16:15 COFFEE BREAK

16:15 – 17:30 Thursday afternoon session II: Cancer biology

Chairs: Achim KRUEGER & Klaudia BRIX

16:15 – 16:27 Roberta BURDEN (Queen's University Belfast) Cathepsin S mediated regulation of the tumour microenvironment is in part facilitated by the transcriptional regulation of CCL2

16:27 – 16:39 Richard WILKINSON (Queen's University Belfast) Reversible cathepsin S inhibitor I6 inhibits tumour growth and progression in vitro and in vivo

16:39 – 16:51 Janja ZAVRŠNIK (Jožef Stefan Institute, Ljubljana) The role of cystatin C depletion in mouse mammary tumor progression and metastasis

16:51 – 17:03 Alejandro GOMEZ-AULI (University of Freiburg) Lysosomal cathepsins regulate the abundance of Cellular Repressor of E1A stimulated Genes 1

17:03 – 17:15 Sarah Marie BRAUN (University of Freiburg) Differential protease regulation as one hallmark of murine breast cancer stem cell plasticity

17:15 – 17:27 Ping WANG (Technische Universität München) OV-MZ-6 ovarian cancer cells overexpressing kallikrein-related peptidases 4, 5, 6 and 7 (KLK4-7): effects on mRNA expression of cancer-related genes

18:00 DINNER (PARADIES, ROSE)

20:00 – 23:45 NIGHT SESSION (CITY HALL)

FRIDAY, FEBRUARY 27

09:00 – 10:40 Friday morning session I: Meprin & Neurodegeneration

09:00 – 09:10 Chairs: Christoph BECKER-PAULY & Walter STÖCKER: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

09:10 – 09:22 Valentina BIASIN (Ludwig Boltzmann Institute for Lung Vascular Research, Graz) Role of meprin β in lung fibrosis

09:22 – 09:34 Johannes PROX (University of Kiel) Ectodomain shedding of CD99 by the metalloprotease meprin β, implicating a function in leukocyte transmigration

09:34 – 09:43 Felix JÄCKLE (University of Kiel) Activity of membrane bound metalloprotease Meprin β is regulated by matriptase 2

09:43 – 09:55 Stephan SCHILLING (Fraunhofer Institute for Cell Therapy and Immunology, Halle/Saale) Heterologous expression of human Meprin β, an alternative β-site cleaving enzyme of APP, in Pichia pastoris

09:55 – 10:07 Frederike SCHMIDT (University of Kiel) Tetraspanin 8 attenuates meprin β activity thereby decreasing amyloid β generation

10:07 – 10:16 Anett KETSCHER (University of Freiburg) A novel conditional mouse model to study tissue-specific functions of Cathepsin D in vivo

10:16 – 10:28 Friederike ZUNKE (University of Kiel) LIMP-2 expression is critical for proteolytic α-synuclein clearance

10:28 – 10:40 Timon LANDINGER (Ludwig-Maximilians-University Munich) Contractile cell plasticity in HtrA1 protease-related cerebral small vessel disease

10:40 – 11:10 COFFEE BREAK

11:10 – 13:00 Friday morning session II: ADAMs & Metalloproteases

11:10 – 11:20 Chairs: Yoshi ITOH & Ed STURROCK: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

11:20 – 11:32 Pascal SCHLAGE (ETH Zurich) Monitoring matrix metalloproteinase 10 activity at the epidermal-dermal junction by SILAC-iTRAQ-TAILS

11:32 – 11:44 Miryam MÜLLER (University of Kiel) A disintegrin and metalloprotease 10 (ADAM10) is a central regulator of murine liver tissue homeostasis.

11:44 – 11:56 Silvio WEBER (Technische Universität Dresden) Cardiomyocyte-specific loss of A Disintegrin And Metalloprotease 10 (ADAM10) leads to functional impairment of the murine heart

11:56 – 12:08 Sebastian WETZEL (University of Kiel) The metalloprotease ADAM10 is an essential driver of podocyte damage

12:08 – 12:20 Chun-Yao YANG (University of Oxford) Charactering Enzyme Kinetics of ADAM15- a Novel Chondroprotective Metalloproteinase in Osteoarthritis.

12:20 – 12:29 Philipp ARNOLD (University of Kiel) Meprin β acts as a sheddase of β1-Integrin thereby inducing intracellular signaling

12:29 – 12:41 Rielana WICHERT (University of Kiel) Meprin β regulates shedding of ADAM10 via activation of ADAM9

13:00 LUNCH (Paradies, Rose)

FRIDAY AFTERNOON SESSIONS: SUBSTRATES & ENZYME MECHANISMS

14:30 – 16:00 Friday afternoon session I: Substrates

14:30 – 14:40 Chairs: Pitter HUESGEN & Ulrich AUF DEM KELLER: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

14:40 – 14:52 Fabian E. EGLI (ETH Zurich) ProteasePredictor: assigning proteases to experimentally derived cleavage sites.

14:52 – 15:04 Fabio SABINO (ETH Zurich) Wound fluid degradomics: system-level insight into protease dynamics in cutaneous wound healing

15:04 – 15:16 Melanie FÖLL (University of Freiburg) Lighting up the darkness behind Prostasin. 15:16 – 15:23 Angelina HAHLBROCK (University Medical Center of Mainz) (High-throughput) cell

based assays to profile protease’s activity and chemical modulators

15:23 – 15:35 Wioletta RUT (Wroclaw University of Technology) Hybrid combinatorial libraries as a tool for optimizing the substrate specificity of human proteasome

15:35 – 15:47 Marcin POREBA (Wroclaw University of Technology) HyCoSuL approach for the optimization of cathepsin L substrate specificity

15:47 – 16:02 Ruth GEISS-FRIEDLANDER (University of Goettingen) Studying the DPP9 interactome

16:02 – 16:30 COFFEE BREAK

16:30 – 18:05 Friday afternoon session II: Substrates & Enzyme Mechanisms

16:30 – 16:40 Chairs: James WHISSTOCK & Guy SALVESEN: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

16:40 – 16:52 Désirée WÜNSCH (University Medical Center of Mainz) Threonine Aspartase1’s evolutionary maturation – lessons learned?

17:52 – 17:04 Birgit WALDNER (University of Innsbruck) Molecular Mechanisms of fXa Substrate Recognition

17:04 – 17:16 Regina FREIER (University of Salzburg) Recognition of birch pollen allergen Bet v 1 by endolysosomal proteases

17:16 – 17:28 Matthias FELTEN (University of Mainz) Functional Characterization of Ovastacin

17:28 – 17:40 Elfriede DALL (University of Salzburg) One enzyme - 2 functions: a refined view on the legumain active site.

17:40 – 17:50 Florian ZAUNER (University of Salzburg) Biochemical characterization of plant legumains

17:50 – 18:05 Gondichatnahalli M. LINGARAJU (Friedrich Miescher Institute & Novartis) Crystal structure of the human COP9 signalosome

18:30 DINNER (PARADIES, ROSE) 20:00 – 23:00 MEETING AND MAKING FRIENDS IN PROTEOLYSIS RESEARCH (PARADIES)

SATURDAY, FEBRUARY 28

09:00 – 10:37 Saturday morning session I: Inhibition strategies

09:00 – 09:10 Chairs: Line HYLTOFT KRISTENSEN & Chris SCOTT: Press release-type introduction into the session: Background, important questions, challenges, solution strategies

09:10 – 09:22 Hauke BUSCH & Melanie BÖRRIES (University of Freiburg) Protease and Protease Inhibitors are Tightly Controlled Through Transcriptional Co-regulation

09:22 – 09:34 Jessica GÖTZFRIED (Helmholtz Zentrum München) Tissue distribution and function of the putative serine protease inhibitor SERPINA2 and its modifying impact on lung diseases

09:34 – 09:46 Therese DAU (Helmholtz Zentrum München) Measurement of active antitrypsin concentration in plasma

09:46 – 09:58 Lisa HINKOFER (Helmholtz Zentrum München) Rezymogenation of PR3 induced by inhibitory mouse and human antibodies

09:58 – 10:10 Sven DAHMS (Leibniz Institute for Age Research, Jena) The structure of apo-furin at 1.8 Å resolution reveals competitive and allosteric inhibitor mechanisms.

10:10 – 10:22 Christian DUBIELLA (Technische Universität München) Selective Inhibition of the Immunoproteasome by Ligand-Induced Crosslinking of the Active Site

10:22 – 10:37 Shirley KNAUER (University of Duisburg-Essen) Targeting of Threonine Aspartase1 by Peptidic Inhibitors

10:37 – 11:10 COFFEE BREAK

11:10 – 12:00 Saturday morning session II: Inhibition strategies

Chairs: Manfred SCHMITT & Oliver SCHILLING

11:10 – 11:22 Lovro KRAMER (Jožef Stefan Institute, Ljubljana) Characterization of potent cathepsin B inhibitors selected from DARPin libraries

11:22 – 11:34 Mario OLF (Johannes Gutenberg-University Mainz) Nephrosin and Fetuin: An Astacin Metalloprotease and its Endogenous Inhibitor

11:34 – 11:46 Christine DOHERTY (University of Oxford) Design of a TIMP-3 mutant with an increased half-life for improved protection against cartilage breakdown

11:46 – 12:00 Roland H. STAUBER (University Medical Center of Mainz) Hit once – better hit twice: An anti-tumoral natural compound with dual activity as a deubiquitinase- and nuclear export-inhibitor

12:15 LUNCH

SATURDAY AFTERNOON: HIKING, EXCURSION, ROUND TABLE DISCUSSIONS

12:15 – 17:00 “Extreme” hiking tour, guided by Thomas REINHECKEL (lunch package will be provided)*

13:00 – 17:00 Relaxed hiking tour, guided by Hans FRITZ*

12:15 – 17:00 Locally organized skiing tours (ski equipment can be rent at the Hotel Paradies; lunch package will be provided)

13:30 – 17:00 Bus tour to Bozen (Visit Ötzi & more)*

* Sign up for the hiking tours and excursion at the administration desk in the lecture hall. The meeting point for the excursions is in front of the hotel Paradies.

19:00 GALA DINNER AT CASTLE PRÖSELS Bus departure time/place will be announced

SUNDAY, MARCH 1

10:00 Bus departure

ABSTRACTS

Available in the printed program only.

LIST OF PARTICIPANTS

THE WINTER SCHOOL FACEBOOK

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Carmen ABFALTER University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria carmen.abfalter@sbg.ac.at +43 662 8044 7212 Philipp ARNOLD University of Kiel Anatomical Institute Otto-Hahn-Platz 8 D-24118 Kiel Germany p.arnold@anat.uni-kiel.de +49 431 8802558 Ulrich AUF DEM KELLER ETH Zurich Institute of Molecular Health Sciences Otto-Stern-Weg 7 CH-8093 Zurich Switzerland ulrich.aufdemkeller@biol.ethz.ch +41 44 633 3392 Nathalie BEAUFORT Klinikum der Universität München Institute for Stroke and Dementia Research Feodor-Lynen-Straße 17 D-81377 Munich Germany nbeaufortgbb@yahoo.fr +49 89 4400 46175 Christoph BECKER-PAULY University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany cbeckerpauly@biochem.uni-kiel.de +49 431 8807118

Valentina BIASIN Ludwig Boltzmann Institute for Lung Vascular Research Stiftingtalstrasse 24 A-8010 Graz Austria valentina.biasin@lvr.lbg.ac.at +43 0316 385 72897 Ewa BIELECKA Jagiellonian University Dept. Microbiology Gornostajowa 7 PL-30-387 Krakow Poland ewa.bielecka@gmail.com +48 12 664 63 87 Wolfram BODE Max Planck Institute of Biochemistry Proteinase Research Group Am Klopferspitz 18 D-82152 Martinsried Germany wolfram.bode@gmx.de +49 89 8504807 Melanie BÖRRIES University of Freiburg Institute of Molecular Medicine and Cell Research Stefan-Meier-Straße 17 D-79104 Freiburg Germany m.boerries@dkfz.de +49 761 203 9626 Hans BRANDSTETTER University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria hans.brandstetter@sbg.ac.at +43 662 8044 7270

The Winterschool Facebook

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Sarah Marie BRAUN University of Freiburg Institute for Molecular Medicine and Cell Research Stefan-Meier-Strasse 17 D-79104 Freiburg Germany sb_braun@t-online.de Klaudia BRIX Jacobs University Bremen Department of Life Sciences and Chemistry Campus Ring 6 D-28759 Bremen Germany k.brix@jacobs-university.de +49 421 200 3246 Roberta BURDEN Queen’s University Belfast School of Pharmacy 97 Lisburn Road Belfast, BT9 7BL Northern Ireland, UK r.burden@qub.ac.uk +44 28 9097 2176 Hauke BUSCH University of Freiburg Institute of Molecular Medicine and Cell Research Stefan-Meier-Straße 17 D-79104 Freiburg Germany hauke.busch@frias.uni-freiburg.de +49 761 2039626 Athena CHALARIS-RISSMANN University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany achalaris@biochem.uni-kiel.de +49 431 8801478

Haissi CUI Technische Universität München Dept. Biochemistry Lichtenbergstraße 4 D-85748 Garching/Munich Germany haissi.cui@mytum.de +49 89 289 12891 Sven O. DAHMS Leibniz Institute for Age Research Fritz Lipmann Institute Jena Beutenbergstraße 21 D-07745 Jena Germany sdahms@fli-leibniz.de +49 3641 656208 Elfriede DALL University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria elfriede.dall@sbg.ac.at +43 662 8044 7278 Therese DAU Helmholtz Zentrum München Comprehensive Pneumology Center Max-Lebsche-Platz 31 D-81377 Munich Germany therese.dau@helmholtz-muenchen.de +49 89 31871653 Christine DOHERTY University of Oxford Kennedy Institute of Rheumatology Roosevelt Drive, Headington OX3 7FY, Oxford United Kingdom christine.doherty@stx.ox.ac.uk +44 1865 612600

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Marcin DRAG Wroclaw University of Technology Dept. Bioorganic Chemistry Wyb. Wyspianskiego 27 PL-50-370 Wroclaw Poland marcin.drag@pwr.wroc.pl +48 71 320 4526 Christian DUBIELLA Technische Universität München Dept. Biochemistry Lichtenbergstraße 4 D-85748 Garching/Munich Germany christian.dubiella@mytum.de +49 89 289 13354 Grzegorz DUBIN Jagiellonian University Malopolska Centre of Biotechnology Gornostajowa 7a PL-30-387 Krakow Poland grzegorz.dubin@uj.edu.pl +48 12 6646362 Fabian EGLI ETH Zurich Institute of Molecular Health Sciences Otto-Stern-Weg 7 CH-8093 Zurich Switzerland fabian.egli@biol.ethz.ch +41 44 633 3392 Han FANG University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria han.fang@stud.sbg.ac.at +43 662 8044 7200

Matthias FELTEN University of Mainz Dept. Cell and Matrix Biology Johann-Joachim-Becher Weg 9-11 D-55128 Mainz Germany feltenm@uni-mainz.de +49 6131 39 24271 Melanie FÖLL University of Freiburg Institute for Molecular Medicine and Cell Research Stefan Meier Strasse 17 D-79104 Freiburg Germany melanie.foell@mol-med.uni-freiburg.de +49 761 203 9614 Regina FREIER University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria regina.freier@sbg.ac.at +43 662 8044 7277 Hans FRITZ Ludwig-Maximilians-University Munich Dept. Clinical Chemistry and Clinical Biochemistry Nußbaumstraße 20 D-80336 Munich Germany fritz@med.uni-muenchen.de +49 8178 1534 Ruth GEISS-FRIEDLANDER University Medical Center of Goettingen Dept. Molecular Biology Humboldtallee 23 D-37073 Goettingen Germany rgeiss@gwdg.de +49 55 139 5984

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Przemyslaw GOLIK Jagiellonian University Malopolska Centre of Biotechnology Gornostajowa 7a PL-30-387 Krakow Poland przemekgolik87@gmail.com +48 12 609886426 Alejandro GOMEZ-AULI University of Freiburg Institute of Molecular Medicine and Cell Research Stefan-Meier-Straße 17 D-79104 Freiburg Germany alejandro.gomez@sgbm.uni-freiburg.de +49 761 203 9603 Jessica GÖTZFRIED Helmholtz Zentrum München Comprehensive Pneumology Center Max-Lebsche-Platz 31 D-81377 Munich Germany jessica.goetzfried@helmholtz-muenchen.de +49 89 31871686 Michael GROLL Technische Universität München Dept. Biochemistry Lichtenbergstraße 4 D-85748 Garching/Munich Germany michael.groll@ch.tum.de +49 89 289 13361 Maresa GRUNDHUBER Ludwig-Maximilians-University Munich Institute of Laboratory Medicine Nussbaumstraße 20 D-80336 Munich Germany maresa.grundhuber@med.uni-muenchen.de +49 89 4400 52597

Barbara GRÜNWALD Klinikum rechts der Isar der TU München Institut für Experimentelle Onkologie und Therapieforschung Ismaningerstraße 22 D-81675 Munich Germany barbara.gruenwald@lrz.tum.de +49 89 4140 4459 Carla GUARINO Centre d'Etude des Pathologies Respiratoires INSERM U1100/EA6305 Faculté de médecine de Tours 10 Bd Tonnellé F-37032 Tours France carlaguarinoo@gmail.com +33 2 47 36 62 53 Shihui GUO University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria shihuiguo@gmail.com +43 662 8044 7283 Angelina HAHLBROCK University Medical Center of Mainz Molecular and Cellular Oncology, ENT Langenbeckstr. 1 D-55131 Mainz Germany angelinahahlbrock@uni-mainz.de +49 6131 17 6030

Yveline HAMON Centre d'Etude des Pathologies Respiratoires INSERM U1100/EA6305 Faculté de médecine de Tours 10 Bd Tonnellé F-37032 Tours France yv.hamon@gmail.com +33 2 47 36 62 53

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Lisa HINKOFER Helmholtz Zentrum München Comprehensive Pneumology Center Max-Lebsche-Platz 31 D-81377 Munich Germany lisa.hinkofer@helmholtz-muenchen.de +49 89 31871627 Caroline HOPPE Jacobs University Bremen Dept. Life Sciences and Chemistry Campus Ring 1 D-28759 Bremen Germany c.hoppe@jacobs-university.de +49 163160 9056 Pitter HUESGEN Forschungszentrum Jülich Central Institute for Engineering, Electronics and Analytics, ZEA-3 Analytics Wilhelm-Johnen-Str D-52428 Jülich Germany p.huesgen@fz-juelich.de +49 2461 61 6873 Line HYLTOFT KRISTENSEN University of Salzburg Dept. Molecular Biology & Novo Nordisk Biopharmaceutical Research Unit Novo Nordisk Park DK-2760 Måløv Denmark lnhk@novonordisk.com +45 3079 8035 Yoshifumi ITOH University of Oxford Kennedy Institute of Rheumatology Roosevelt Drive, Headington Oxford OX3 7FY United Kingdom yoshi.itoh@kennedy.ox.ac.uk +44 1865 612647

Felix JÄCKLE University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany felix.jaeckle@me.com +49 157 543 688 50 Dieter JENNE Helmholtz Center Munich & Max-Planck Institute of Neurobiology Max-Lebsche-Platz 31 D-81377 Munich Germany dieter.jenne@helmholtz-muenchen.de +49 89 31874664 Konstantin KARMILIN University of Mainz Dept. Cell and Matrix Biology Johann-Joachim-Becher Weg 9-11 D-55128 Mainz Germany karmilik@uni-mainz.de +49 6131 39 24271 Nežka KAVČIČ Jožef Stefan Institute Dept. Biochemistry, Molecular and Structural Biology Jamova cesta 39 SI-1000 Ljubljana Slovenia nezka.kavcic@ijs.si +3861 477 3753 Anna KEPPNER University of Lausanne Dept. Pharmacology and Toxicology Rue du Bugnon 27 CH-1005 Lausanne Switzerland anna.keppner@unil.ch + 41 21 692 54 26

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Anett KETSCHER University of Freiburg Institute of Molecular Medicine and Cell Research Stefan-Meier-Straße 17 D-79104 Freiburg Germany anett.ketscher@mol-med.uni-freiburg.de +49 761 203 9505 Shirley KNAUER University Duisburg-Essen Molecular Biology II Universitätsstrasse 5 D-45141 Essen Germany shirley.knauer@uni-due.de +49 201 183 4987 Tobias KOCKMANN ETH Zurich Institute of Molecular Health Sciences Otto-Stern-Weg 7 CH-8093 Zurich Switzerland tobias.kockmann@biol.ethz.ch +41 44 633 3392 Brice KORKMAZ Centre d'Etude des Pathologies Respiratoires INSERM U1100/EA6305 Faculté de médecine de Tours 10 Bd Tonnellé F-37032 Tours France brice.korkmaz@inserm.fr +33 2 47 36 62 53 Ludwig KORNREITER Omnibusunternehmen Habermayr Schleifmühlweg 14 D-86633 Neuburg/Donau Germany omnibus.habermayr@t-online.de +49 171 6519414

Lovro KRAMER Jožef Stefan Institute Dept. Biochemistry, Molecular and Structural Biology Jamova 39 SI-1000 Ljubljana Slovenia lovro.kramer@ijs.si +386 1477 35 10 Eileen KROLL Jacobs University Bremen Dept. Life Sciences and Chemistry Campus Ring 1 D-28759 Bremen Germany e.kroll@jacobs-university.de +49 421 200 3584 Achim KRUEGER Klinikum rechts der Isar der Technischen Universität München Institut für Molekulare Immunologie und Experimentelle Onkologie Ismaningerstraße 22 D-81675 Munich Germany achim.krueger@lrz.tu-muenchen.de +49 89 4140 4463 Katarzyna KUŚKA Jagiellonian University Malopolska Centre of Biotechnology Gornostajowa 7a PL-30-387 Krakow Poland kuska.kk@gmail.com +48 792516779 Timon LANDINGER Ludwig-Maximilians-University Munich Institute for Stroke and Dementia Research Feodor-Lynen-Straße 17 D- 81377 Munich Germany timon.landinger@med.uni-muenchen.de +49 89 4400 48174

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Michaela LASSACHER University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria michaela.lassacher@sbg.ac.at +43 662 8044 7200 Christine LEHMANN MRC National Institute for Medical Research Division of Parasitology The Ridgeway, Mill Hill London NW7 1AA United Kingdom clehman@nimr.mrc.ac.uk +44 20 8816 2668 Gondichatnahalli M. LINGARAJU Friedrich Miescher Institute for Biomedical Research Maulbeerstrasse 66 CH-4058 Basel Switzerland lingaraju.gm@fmi.ch + 41 76 4714413 Viktor MAGDOLEN Frauenklinik der TU München Klinische Forschergruppe Klinikum rechts der Isar Ismaningerstraße 22 D-81675 Munich Germany viktor.magdolen@lrz.tum.de +49 89 4140 2493 Miryam MÜLLER University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany mmueller@biochem.uni-kiel.de +49 431 8807112

Michael NIEMEYER Fraunhofer Institute for Cell Therapy and Immunology (IZI) Dept. Drug Design and Target Validation (MWT) Biocenter Weinbergweg 22 D-06120 Halle (Saale) Germany michael.niemeyer@izi.fraunhofer.de +49 345 131428 00 Anke OHLER University of Mainz Dept. Cell and Matrix Biology Johannes-von-Müller-Weg 6 D-55124 Mainz Germany anke.ohler@uni-mainz.de +49 6131 39 26656 Mario OLF University of Mainz Dept. Cell and Matrix Biology Johannes-Joachim-Becher Weg 9-11 D-55128 Mainz Germany olf@uni-mainz.de +49 6131 24271 Aleksandra PECAK Jagiellonian University Malopolska Centre of Biotechnology Gornostajowa 7a PL-30-387 Krakow Poland aleksandra.pecak@uj.edu.pl +48 12 664 5369 Christoph PETERS University of Freiburg Institute for Molecular Medicine and Cell Research Stefan-Meier-Strasse 17 D-79104 Freiburg Germany christoph.peters@mol-med.uni-freiburg.de +49 761 203 9601

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Marcin POREBA Wroclaw University of Technology Dept. Bioorganic Chemistry Wyb. Wyspianskiego 27 PL-50-370 Wroclaw Poland marcin.poreba@pwr.wroc.pl +48 71 320 24 55 Johannes PROX University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany jprox@biochem.uni-kiel.de +49 431 8802215 Björn RABE University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany brabe@biochem.uni-kiel.de +49 431 8801678 Yesid A. RAMIREZ University of Wuerzburg Dept. Structural Biology Josef-Schneider-Straße 2 D- 97080 Würzburg Germany yesid.ramirez@uni-wuerzburg.de +49 176 98258000 Thomas REINHECKEL Albert-Ludwigs-University of Freiburg Institute of Molecular Medicine and Cell Research Stefan Meier Straße 17 D-79104 Freiburg Germany thomas.reinheckel@uniklinik-freiburg.de +49 761 203 9606

Martin RENATUS Novartis Institutes for Biomedical Research Fabrikstrasse 2 CH-4002 Basel Switzerland martin.renatus@novartis.com +41 76 440 9958 Steffen RIETHMÜLLER University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany sriethmueller@biochem.uni-kiel.de +49 431 8802215 Wioletta RUT Wroclaw University of Technology Dept. Bioorganic Chemistry Wyb. Wyspianskiego 27 PL-50-370 Wroclaw Poland wioletta.rut@pwr.wroc.pl +48 71 320 24 55 Fabio SABINO ETH Zurich Institute of Molecular Health Sciences Otto-Stern-Weg 7 CH-8093 Zurich Switzerland fabio.sabino@biol.ethz.ch +41 44 633 3392 Guy SALVESEN Sanford-Burnham Medical Research Institute 10901 North Torrey Pines Road La Jolla, CA 92037 USA gsalvesen@sanfordburnham.org +1 858 646 3114

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Magdalena SCHACHERL University of Cologne Institute of Biochemistry Otto-Fischer-Str. 14 D-50674 Cologne Germany magdalena.schacherl@uni-koeln.de +49 221 470 3212 Oliver SCHILLING University of Freiburg Institute for Molecular Medicine and Cell Research Stefan Meier Strasse 17 D-79104 Freiburg Germany oliver.schilling@mol-med.uni-freiburg.de +49 761 2039615 Stephan SCHILLING Fraunhofer Institute for Cell Therapy and Immunology (IZI) Dept. Drug Design and Target Validation (MWT) Biocenter Weinbergweg 22 D-06120 Halle (Saale) Germany stephan.schilling@izi.fraunhofer.de +49 345 131428 00 Pascal SCHLAGE ETH Zurich Institute of Molecular Health Sciences Otto-Stern-Weg 7 CH-8093 Zurich Switzerland pascal.schlage@biol.ethz.ch +41 44 633 3392 Frederike SCHMIDT University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany fschmidt@biochem.uni-kiel.de +49 431 8802215

Stefanie SCHMIDT University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany stschmidt@biochem.uni-kiel.de +49 431 8801478 Dirk SCHMIDT-ARRAS University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany darras@biochem.uni-kiel.de +49 431 8807112 Manfred SCHMITT Technische Universität München Dept. Obstetrics and Gynecology (Frauenklinik) Ismaningerstraße 22 D-81675 München Germany me-schmitt@t-online.de +49 89 4140 2449 Esther SCHÖNAUER University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria esther.schoenauer@sbg.ac.at +43 662 8044 7277 Øyvind L. SCHÖNBERGER Munich kontakt@oeyvind-schoenberger.de +43 152 0643 4404

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Kristin SCHUBERT University of Oxford Kennedy Institute of Rheumatology Roosevelt Drive, Headington OX3 7FY, Oxford United Kingdom kristin.schubert@kennedy.ox.ac.uk +44 1865 612600 Neele SCHUMACHER University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany nschumacher@biochem.uni-kiel.de +49 431 8801478 Jeanette SCHWARZ University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany jschwarz@biochem.uni-kiel.de +49 431 8801478 Christopher SCOTT Queen’s University Belfast School of Pharmacy 97 Lisburn Road Belfast, BT9 7BL Northern Ireland, UK c.scott@qub.ac.uk +44 28 9097 2350 Pernille SØGAARD University of Oxford Kennedy Institute of Rheumatology Roosevelt Drive, Headington OX3 7FY, Oxford United Kingdom pia.sogaard@ndorms.ox.ac.uk +44 7450645204

Christian SOMMERHOFF Klinikum der Universität München Institute of Laboratory Medicine Nussbaumstraße 20 D-80336 Munich Germany sommerhoff@med.uni-muenchen.de +49 89 4400 55153 Roland H. STAUBER University Medical Center of Mainz Molecular and Cellular Oncology Langenbeckstr. 1 D-55131 Mainz Germany rstauber@uni-mainz.de +49 6131 17 7002 Walter STÖCKER University of Mainz Institute of Zoology, Cell and Matrix Biology Johannes-von-Müller-Weg 6 D-55128 Mainz Germany stoecker@uni-mainz.de +49 6221 3924273 Ed STURROCK University of Cape Town Institute of Infectious Disease and Molecular Medicine Suite 3.13, Wernher and Beit Building Anzio Road, Observatory 7925 South Africa edward.sturrock@uct.ac.za +27 21 4066312 Claudia TREDUP University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany cbroder@biochem.uni-kiel.de +49 176 32531129

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Linda TROEBERG University of Oxford Kennedy Institute of Rheumatology Roosevelt Drive, Headington OX3 7FY, Oxford United Kingdom linda.troeberg@kennedy.ox.ac.uk +44 1865 612600 Christian TÜTING Fraunhofer Institute for Cell Therapy and Immunology (IZI) Dept. Drug Design and Target Validation (MWT) Biocenter Weinbergweg 22 D-06120 Halle (Saale) Germany christian.tütting@izi.fraunhofer.de +49 345 131428 00 Marie-Theres VIELBERG Technische Universität München Dept. Biochemistry Lichtenbergstraße 4 D-85748 Garching/Munich Germany marie-theres.vielberg@mytum.de +49 89 289 13354 Rudolf WACHTEL Technische Universität München Dept. Chemistry Lichtenbergstraße 4 D-85748 Garching/Munich Germany rudolf.wachtel@mytum.de +49 89 289 13744 Birgit WALDNER University of Innsbruck Institute of General, Inorganic and Theoretical Chemistry Innrain 82 A-6020 Innsbruck Austria birgit.waldner@uibk.ac.at +43 512 507 57137

Ping WANG Technische Universität München Klinische Forschergruppe der Frauenklinik Ismaningerstraße 22 D-81675 Munich Germany wang_ping_2010@hotmail.com +49 89 4140 7412 Silvio WEBER Technische Universität Dresden Dept. Pharmacology and Toxicology Fetscherstraße 74 D-01307 Dresden Germany silvio.weber@tu-dresden.de +49 351 458 6278 Hagen WESTPHAL University of Mainz Dept. Cell and Matrix Biology Johann-Joachim-Becher Weg 9-11 D-55128 Mainz Germany hagen.westphal@uni-mainz.de +49 6131 39 24271 Sebastian WETZEL University of Kiel Institute of Biochemistry Otto-Hahn-Platz 9 D-24118 Kiel Germany swetzel@biochem.uni-kiel.de +49 431 880 2219 James C. WHISSTOCK Monash University Dept. Biochemistry & Molecular Biology Bldg 77, Wellington Road Clayton, Victoria 3800 Australia james.whisstock@monash.edu +61 3 9902 9312

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Rielana WICHERT University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany rwichert@biochem.uni-kiel.de +49 431 8802215 Richard WILKINSON Queen’s University Belfast School of Pharmacy 97 Lisburn Road Belfast, BT9 7BL Northern Ireland, UK rwilkinson04@qub.ac.uk +44 28 9097 2164 Désirée WÜNSCH University Medical Center of Mainz Molecular and Cellular Oncology, ENT Langenbeckstr. 1 D-55131 Mainz Germany wuensch@uni-mainz.de +49 6131 17 6030 Chun-Yao YANG University of Oxford Kennedy Institute of Rheumatology Roosevelt Drive, Headington OX3 7FY, Oxford United Kingdom chun-yao.yang@lincoln.ox.ac.uk +44 1865 612600 Florian ZAUNER University of Salzburg Dept. Molecular Biology Billrothstraße 11 A-5020 Salzburg Austria florian.zauner@sbg.ac.at +43 662 8044 7279

Janja ZAVRŠNIK Jožef Stefan Institute Dept. Biochemistry, Molecular and Structural Biology Jamova 39 SI-1000 Ljubljana Slovenia janja.zavrsnik@ijs.si +386 1 4773 510 Friederike ZUNKE University of Kiel Institute of Biochemistry Rudolf-Höber-Straße 1 D-24118 Kiel Germany fzunke@biochem.uni-kiel.de +49 431 8802219

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