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In Microfluidics 1.n, volumes were small, times fast, but there was a lot of “macro” equipment needed to run the “micro” parts (e.g. pumps, valves, manifolds—good but still expensive.

In macro-scale chemistry volumes were big, and for sequential reactions, times slow—good but expensive.

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Paul Yager UW, PI Immunoassays

Patrick Stayton UW, Biomolecular systems development

Walt Mahoney Epoch Biosciences NA Assay development

Fred Battrell Micronics Lab card/reader dev. Systems integration, Commercialization

Gonzalo Domingo PATH Assay validation; UNA, Sample procurement

Rick Gardner Invetech Instrument design/build

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Pathogen class

Disease

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RNA virus Dengue RNA and IgM should work, need better IA reagents

RNA virus Measles RNA and IgM work

RNA virus Influenza Blood is the wrong sample for virus

Single IgM sample not definitive Protozoan Parasite Malaria RNA better than microscopy

2 antigens work

Bacterium Typhoid IgM OK RNA copy number too low in blood

Bacterium Rickettsia IgM OK RNA copy number too low in blood

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Personal Health Information Flow

Clinics and Hospitals

Specialists

Image-readable disposable bioassay

devices

Emergency Room

Primary Care Provider

Where the patient is

Personal Medical

Databases

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INTERNET

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