yager cbm4b 2011 for web site.pptx
TRANSCRIPT
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In Microfluidics 1.n, volumes were small, times fast, but there was a lot of “macro” equipment needed to run the “micro” parts (e.g. pumps, valves, manifolds—good but still expensive.
In macro-scale chemistry volumes were big, and for sequential reactions, times slow—good but expensive.
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Paul Yager UW, PI Immunoassays
Patrick Stayton UW, Biomolecular systems development
Walt Mahoney Epoch Biosciences NA Assay development
Fred Battrell Micronics Lab card/reader dev. Systems integration, Commercialization
Gonzalo Domingo PATH Assay validation; UNA, Sample procurement
Rick Gardner Invetech Instrument design/build
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Pathogen class
Disease
Lessons
RNA virus Dengue RNA and IgM should work, need better IA reagents
RNA virus Measles RNA and IgM work
RNA virus Influenza Blood is the wrong sample for virus
Single IgM sample not definitive Protozoan Parasite Malaria RNA better than microscopy
2 antigens work
Bacterium Typhoid IgM OK RNA copy number too low in blood
Bacterium Rickettsia IgM OK RNA copy number too low in blood
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Optical detection of analytes in parallel by NAAT and IA on the same device Disposables for NAAT and IA contained all reagents (wet and dry) Initial sample a finger stick blood sample
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In Microfluidics 1.n, volumes were small, times fast, but there was a lot of “macro” equipment needed to run the “micro” parts (e.g. pumps, valves, manifolds—good but still expensive.
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In macro-scale chemistry volumes were big, and for sequential reactions, times slow—good but expensive.
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Clinics and Hospitals
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Personal Medical
Databases
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$1M 2-year project (2009-2011) to demonstrate a sensitive instrument-free multiplexed diagnostic platform using disposable 2DPNs for use in low-resource settings
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Publication in progress: Osborn, J. L. et al., LOC,2011
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