UDK 6329 YU ISSN 0372-7866I I INSTITUT ZA ZA$TITU BIUA - BEOGRAD

INSTITUTE FOR PLANT PROTECTION - BEOGRAD

ZASTITA BILJA (PLANT PROTECTION)

VOL 32(1) BROJ 155 1981 GOD

Za~tita b ilj a Vol 32(1) Br 155 (str 1-104) Beograd 1981

Zastita bhlja Vol 32(1) br 155 1-104 (1981) Beograd

CONTENTS

Scientific papers

D Stojanobullic K Mijatovic B Boric Some species of Cuscuta L which are less spread on the territory of Serbia Vojvodina and Macedonia - 11

S E Fayzalla A Mamiddotic Contribution to the study of biology and epidemiology of Phoma macdoualdii Boerema causer of black spot of sunflower - - 26-27

S Stojanovic M Azdrejic Biotypes of physiological races of Puccinia graminis tritici isolated from grasses 34

I Cizmic I MilatoviC J KispatiC

Das Aultreten des Pilzes Rhizoctonia solani KUhn als Ursache der Halmfaule an Weizen und Gerste - 38

J Cota R Numic A contribution to the Knowledge of the Most Important Causers of Diseases of the Snap Beans on the Territory of Lijevac Poljc - 44

2 Aleksic N Marinkovic Tobacco mosaic vlrus on some pepper (Capsicum annum L) variemiddot ties under cond~tions of natural infection - - - - middot- 54

M Rankovic S Vuksanovic

Enzyme-linked immunosorbent assay (ELISA) in the -detecting Sharshyka virus - - - - - - - - - - - - 60

2 Peric B Randelovic V Vojinovic N N esectkovic Investigations of pesticide residue levels in foodstuffs in Serbia (1979-1980) - - - - - - 70

L MiriinskiStefanoviC Effect of herbicides on tbullhe growth rate and yield on maize inbred amp~ - - - - - - - - - - - - - n

D karadiic R Zoric Contribution to the knowledge on the biology of the fungi Naemashycyclus minor Butin - causer of needlecast of scots pine - 89-90

V Lazarev Intensity of the attack of Lophodermium species an the developshyment of the disease on some Yugoslav provenances of scots pineseedlings 99

In memoriam

M Dimitrijevic Prof dr Gojko Pivar - - 103- 104

Zastita bhlja Vol 32(1) br 155 1-104 (1981) Beograd

CONTENTS

Scientific papers

D Stojanobullic K Mijatovic B Boric Some species of Cuscuta L which are less spread on the territory of Serbia Vojvodina and Macedonia - 11

S E Fayzalla A Mamiddotic Contribution to the study of biology and epidemiology of Phoma macdoualdii Boerema causer of black spot of sunflower - - 26-27

S Stojanovic M Azdrejic Biotypes of physiological races of Puccinia graminis tritici isolated from grasses 34

I Cizmic I MilatoviC J KispatiC

Das Aultreten des Pilzes Rhizoctonia solani KUhn als Ursache der Halmfaule an Weizen und Gerste - 38

J Cota R Numic A contribution to the Knowledge of the Most Important Causers of Diseases of the Snap Beans on the Territory of Lijevac Poljc - 44

2 Aleksic N Marinkovic Tobacco mosaic vlrus on some pepper (Capsicum annum L) variemiddot ties under cond~tions of natural infection - - - - middot- 54

M Rankovic S Vuksanovic

Enzyme-linked immunosorbent assay (ELISA) in the -detecting Sharshyka virus - - - - - - - - - - - - 60

2 Peric B Randelovic V Vojinovic N N esectkovic Investigations of pesticide residue levels in foodstuffs in Serbia (1979-1980) - - - - - - 70

L MiriinskiStefanoviC Effect of herbicides on tbullhe growth rate and yield on maize inbred amp~ - - - - - - - - - - - - - n

D karadiic R Zoric Contribution to the knowledge on the biology of the fungi Naemashycyclus minor Butin - causer of needlecast of scots pine - 89-90

V Lazarev Intensity of the attack of Lophodermium species an the developshyment of the disease on some Yugoslav provenances of scots pineseedlings 99

In memoriam

M Dimitrijevic Prof dr Gojko Pivar - - 103- 104

Zastita biljao Vol 32(1) br 155 5-ll (15~81 Beogra~---- l1

SOME SPECIES OF CUSCUTA L WHICH ARE LESS SPREAD ON THE TERRITORY OF SERBIA VOJVODINA AND MACEDONIA

by

D Stojanovlc K Mljatovic B Borlc

Institute for Plant Protection Btogmd

Summary

In the course of several years was effected the identification of the species belonging to the genus Cuscuta on cultivated plants and on the spontaneous flora on the territory of Serbia Vojvodina and Macedonia On this occasion were observed 7 species which occur sporadically as fallow Cuscuta monogyna Vahl C lupuliformis Krock C pentagona Eng C tinei Inzenga C approximata Bah C epithymum Murr and C europaea L There were identified also the following vamiddotrieties Cuscushyta monogyna vabullr typica Buia C pentagona var typica Buia C approshyximata var typica Buia C approximata var leucosphaera (Bois s et He I d r) Yunck C epithymum va-r typica Beck C europaea var coshynocarpa Eng C europaea var nefrens Fries and C europaea var vishyciae Eng

C pentagona C approximata and C epithymum were found on clover alfalfa and spontaneous flora whemiddotreas the other species were found only on spontaneous flora

26 Zastita b jlja Vol 3ZI ) br 155 13-27 0981) Beograd

davina i duzim SIUsnim perioclima tokom vegetacij e Nedostatak vlage u zemljitu u odreden~m peniodima rauvoja suncokreta verovatno u tiee na slabljenje biljaka i povecanje osetljivosti prema parazHu

(Primljeno 23 OJ 1981

LIT ERATU RA

Acim o v ~ c M (1965) Phoma sp novi parazit sunookreta u JugoslavJji Zbor radova lnst za polj istraz N Sad br 3 151- 170

A 1 e k see v-a S P (1969 Fomoz podsolnechnika Zast Rast Moskva 14 8 48 Ilt r e z z i M J (1964) Especie d el gtmero Phoma purasita de Heliant11Us annus L

en Manfredi (Cordoba) RepubHa Ar-gentina ldia 37-40 G u ill au min J J ct P i c r son J (1976) Le tourncsol une oulwre en exstenshy

sioo et ses maJadies crytogamiques Phytoma 28 S- 11 Lu ciano A at D a v r e u x M (1967) Produccion de Giraso1cn en Argentina

Pub tee Estac exp Pergamino 37 Vii 53 pp Ma r i c A und S c h n cider R (1979) Die Schwarzfleckenkrankhcit der Sonnshy

enblume in Jugoslawien und Ihr Erreger Phoma macdonalcli Boerema Physhytopathot Z 94 22b-233 Berb1

M o ric A I sIa m U F ay z a II a S i M a~ ire vi c S (1979) Stotnost mrke (A helianthi) a erne pegavosti (Ph rnacdonaldi) suncokrota i mogucnost~ njihoshyvog suzbijanja hemijsJcim sredstvmiddotima I Jug Savet o prim pesticida u zat biljo Zbornlk radova sv l 229-236 Kupari

M c Don a 1 d W C (1964) Plwme black stem of sunflowers Phytopathology S4 492-493

Tircomnicu M N liem ann E and Ilicscu H (1972) Boli noi pe floareamiddot -soarelui a nova boa~a in Romania Probl Agr 23 74-77

CONTRIBUTION TO THE STUDY OF BIOLOGY AND EPIDEMIOLOGY OF PHOMA MACDONALD BOREMA CAUSER OF BLACK SPOT

OF SUNFLOWER by

Fayza))li E S and Marl~ A

Faculty of Agriculture Novi Sad Yugoslavia

Summary

middotphoma black spot has beoome very harmfuJl middotdisease of sunf~ower in Yu goslavia H appeaors every year some times caJuSiimg great decrease in yield of seed

Ln this paper the resuJts of four years investigation on some bioloshygical characteristics and epidemiology of Phoma macdonaldi have been found that there ~s a great vaJl1iabilrty irn the pathogemiidty between the diffe rent isolaltes of middottthe fungus It has been for othe First 1i-me disoowed that transmissmiddotion of the parasite occurs from one seaSJOn oto other also by~ seed middot~tself middot middot

26 Zastita b jlja Vol 3ZI ) br 155 13-27 0981) Beograd

davina i duzim SIUsnim perioclima tokom vegetacij e Nedostatak vlage u zemljitu u odreden~m peniodima rauvoja suncokreta verovatno u tiee na slabljenje biljaka i povecanje osetljivosti prema parazHu

(Primljeno 23 OJ 1981

LIT ERATU RA

Acim o v ~ c M (1965) Phoma sp novi parazit sunookreta u JugoslavJji Zbor radova lnst za polj istraz N Sad br 3 151- 170

A 1 e k see v-a S P (1969 Fomoz podsolnechnika Zast Rast Moskva 14 8 48 Ilt r e z z i M J (1964) Especie d el gtmero Phoma purasita de Heliant11Us annus L

en Manfredi (Cordoba) RepubHa Ar-gentina ldia 37-40 G u ill au min J J ct P i c r son J (1976) Le tourncsol une oulwre en exstenshy

sioo et ses maJadies crytogamiques Phytoma 28 S- 11 Lu ciano A at D a v r e u x M (1967) Produccion de Giraso1cn en Argentina

Pub tee Estac exp Pergamino 37 Vii 53 pp Ma r i c A und S c h n cider R (1979) Die Schwarzfleckenkrankhcit der Sonnshy

enblume in Jugoslawien und Ihr Erreger Phoma macdonalcli Boerema Physhytopathot Z 94 22b-233 Berb1

M o ric A I sIa m U F ay z a II a S i M a~ ire vi c S (1979) Stotnost mrke (A helianthi) a erne pegavosti (Ph rnacdonaldi) suncokrota i mogucnost~ njihoshyvog suzbijanja hemijsJcim sredstvmiddotima I Jug Savet o prim pesticida u zat biljo Zbornlk radova sv l 229-236 Kupari

M c Don a 1 d W C (1964) Plwme black stem of sunflowers Phytopathology S4 492-493

Tircomnicu M N liem ann E and Ilicscu H (1972) Boli noi pe floareamiddot -soarelui a nova boa~a in Romania Probl Agr 23 74-77

CONTRIBUTION TO THE STUDY OF BIOLOGY AND EPIDEMIOLOGY OF PHOMA MACDONALD BOREMA CAUSER OF BLACK SPOT

OF SUNFLOWER by

Fayza))li E S and Marl~ A

Faculty of Agriculture Novi Sad Yugoslavia

Summary

middotphoma black spot has beoome very harmfuJl middotdisease of sunf~ower in Yu goslavia H appeaors every year some times caJuSiimg great decrease in yield of seed

Ln this paper the resuJts of four years investigation on some bioloshygical characteristics and epidemiology of Phoma macdonaldi have been found that there ~s a great vaJl1iabilrty irn the pathogemiidty between the diffe rent isolaltes of middottthe fungus It has been for othe First 1i-me disoowed that transmissmiddotion of the parasite occurs from one seaSJOn oto other also by~ seed middot~tself middot middot

27 Zastita bHja Vol 32(1) br ISS 13-27 (1981) Beograd

The middotinfection takes place in susceptible genotype by fungus under saturated atmosphere at lSC and minimal exposition period of 48 hours By increasing the e~poslition period up to 72 hours in saturated atmosmiddot phere at 2smiddotc increamiddotse the intensi-ty of infestation

Some of agrotcchnical pracmiddottices have a great influence on the deveshylopment of the disease during sunfLower vegetal~on The ~ntenSiioty of disease was regularly much higher in early sowing crops (from beginnshying of March till middle of April) Phoma black spot attack wamiddots olosely related to middotthe quantity of N P K nutrients and iots time of application Applicatmiddot1on of higher doses of Nitrogen were middotin correlation with the more intensive development of the disease Great differences in a-ttack of disease on different sunflower fields of the same localtty could be explaind by different agrootechnioal managment

The first sympmiddottoms of the disease usually appear in the middle July in the area of Vojrodina the main growing bullregion of sunflower in Yugoslavia The lowesbullt infestion of Phoma black spot of SIUnflower was observed in 1977 It was characterized by very Mgh quantioty and go~d diS~tribution of prectiPtion of rainfall from beginning to middotthe end middotof vegemiddot tation period The greatest damages of disease was found middot~n 1979 -due to unfavorable weathe-r condition especially because of three dry middotperiods during vegetation So the water stress seems to be very important faotor in weak1ng the plants which makes them more suscepotible to the middotdisease

34 Zastita bilja V-ol ~2(1) hr 155 29-34 (1981) BO~rad

Hay MoB H A (139 PXltaBltmHa xne6Hldx 3JlaKOB B CCCP Cemxoon19 MocKBa - Jle~mHrp~

Ra no j e vic N (1912) Kriptogamske bolesti hta u Srbiji Beograd Roe 1 f s A P and M c V e y D V (1974) Races of Puccinia graminis f sp tritici

in the USA during 1973 Plant Disease Reported V~ 58 No 7 608-611 Sampson K and Western J H (1954) Diseases of Britich Grasses and

Herbage Legumens Cambridge Sib i 11 a C Basi 1 e R (1959) Graminacee spontanee attacoate da ruggini raemiddot

colte in l talia nel 1959 Boll Staz Pat Veg XVII Serle Terz-a 117- 126 S t a k m a 11 E C Stewart D M and Lo e gering W (1962) Identification

of physiologic races of Puccinia graminis tritici USDA ARS E-ltgt17 S to j an o vic D Kostic B (1956) Prilog proueavanj u parazitne flore na

jednom deLu teritorije uie Srbije Za~tilta bilja 35 87- 103 Spehar V (1975) Epidemiology of wheat rust in Europe Proceeding15 Intemamiddot

tional winter wheat conference pp 435-441 Zagreb

BIOTYPES OF PHYSIOLOGICAL RACES OF PUCCINA GRAMINIS TRITICI ISOLATED FROM GRASES

S Stojanovlc and M Andrejlc

Summary

In the period from 1975 to 1979 oin the Institute for Small Grains at Kragujevac 122 isolates of the parasitic fungus Puccinia graminis tritici from grasses were examined middot

In the southeastern part of Yugoslavia this fungus was found to grow on fifteen grasses Hordeum m urinum H spontaneum H vilshylosa H mari11um H leporinum Lolium perenne Aegilops cylindrica A variabilis A ventricosa A ovata A longissima A binucialis A charonensis Bromus rigens and Agropyrum repens

From these grasses five races (1 11 34 116 and 194) and six biomiddot types have been identified (RKK RRT RTT RKF RKT and RHT) The dominant races were 11 (5328) and 34 (3770) and biotypes RRT (1803) and RTT (1312) The most virulent in the population was biotype RTT Effective f-or this biotype was only gene Sr 9e

38 Zastita bilia Vol 32(1) br 155 35-38 (1981) Beograd

LITERATURA

Buhl C Weidner H Zogg H (1975) Krankheiten und schadlinge an Gemiddot treide und Mais Stuttgart

Nee r g a a r d P (1977) Seed Pathology London Pitt D (1964) Studies on sharp eyespot disease of cereals Ann appl Bioi 54

77-89 We s t er u J H (1971) D)seases of Crop Plants London W i es s e M V (1977) Compendium of Wheat Diseases Minnesota

DAS AUFTRETEN DES PILZES RHIZOCTON IA SOLAN KOHN ALS URSACHE DER HALMFXULE AN WEIZEN UND GERSTpound

L Clzmtc I MHatovlc J Kilpatlc

Fakultat der Landwirtschafbswissenschaften Zagreb lnstitut ftir Pflanzenschutz

Zusammenfassung

In der Arbeit sind erste Ergebnisse tiber den Befund des Pilzes Rhizoctonia solani KUhn als Ursache der Halmfaule an Weizen und Gerste beschrieben Der Pilz wurde an diesen Getreidearten erstenmal gefestgestellt Die Determination wurde nach den Symptomen und mit Isolierung in Rcinkultur durcbgeftihrt

44 Za~tita bilja Vol 32(1) br 155 39-44 (1981) Beograd

A CONTRIBUTION TO THE KNOWLEDGE OF THE MOST IMPORTANT CAUSERS OF DISEASES OF THE SNAP BEANS ON THE TERRITORY

OF LIJEVAC POLJE

by

J ~ota

UPI OOUR Institute for Crop Fam1ing Butmir - IUlla

R Numlc

Faculty of Agriculture Sarajevo

Summary

On the basis of the published Jnvestigations of sensitivity of three sorts of snap beans (Cordon Harvester and Top Crop) to the infections with the economically most important pathogans Colletotrichum Iindemiddotbull muthianum and Pseudomonas phaseolicola in the conditions of twoshymiddotterms sowing (in spring and in summer) with and without irrigation following conclusions may be drawn

The sort Cordon was the most resistant to the infection with both pathogens because it had the lowest cllsease index with Colletotrichum lindemutlzianum whereas this index was 0 with Pseudomonas phasemiddot olicola

In relation to the infection with Colletotrichum lindemuthianmn the sort Top Crop is somewhat more resistant than the sort Harvester whereas the sensitivity to the infection with Pseudomonas phaseolicola is somewhat higher under the conditions of irrigation

The yield achived with the irrigation was regularly higher than in the culture without middotirrigation although the disease index was higher The sort HaiVester had the highest yield under the conditions without irrigation (in 1977 in the second sowing term 1428 dtha) as well as with irrigation at the same term and in the same year (1924 dtha)

ZaStita biljo Vol 32(1) br JSS SS-60 (1981) Beograd

MOojko RaDJrovlt Svetlana Vuksanovl6 lnslitut za vo6arstvo C~ak

BNliMSKI IMUNOADSORPCIONI TEST (ELISA) ZA DOKAZIVANJE VIRUSA SARKE

U radu je proveravaoa vrednost serolookog testa bullELlshySAc za dakazivanje virusa Salke uporcdo kod osetljivili i tOlerantnih sorti sljiva i kajsije Vrirus je ~eSnO dokazishyvan u UTorcima lisnih pupoljaka ovetnih pupoljaka middotkrushynicnim listicima liScu koni j zrelim plodovJilla

Uvod

U borbi protiv viroza poljopr~vrednih biljaka brza i pouzdana dishyjagnoamptika zauzima jcdno od najznacajnijJh mesta Njen zonaeaj oolazi narooito do izrazaja kod onih virusa kojd sc hrzo Sibullre putem lisnih vashy~iju i koJi zaraiavaju vise bHjnih vrsta sto je s-Jucaj sa virusom sarke za koji je utvrdeno da pored sljdve (Prunus domestica) moze zaraziti jos 23 vrste iz roda Prunus

Dijagnoza prisustva virusa ~arke bullu Mjivi se obavlja namiddotjlakSe vizuelshynim opatanjem simptoma na iScu tokom juna meseoa i na plodovima tokom leta odnosno u vreme njtihovog sazrevmja Mcdutim neke sorte ne middotispoljaVaju simptomemiddotna liScu pa ako nisu stupHe na rod simptomi sene mogu zapaa~i ~ni na plodovdma Isto taQo postJoje sorte middotkoje ce daju simptome ni na li~6u ni na plodovima Pored -toga u nekdm slu6ajemiddot vima javljaju se problcmi raspoznavanja simptoma protwrokovanih vishyrusom arke od simptoma Jinijskog mozaika (Sa v a I e oS k u amp M a coshyvei 1965 1968) in simptcgtma na plodovima tzv raquomiddotIafna Sctrkalaquo (Kegshyler et al 1964 Posnebulltmiddotte amp Ellenber-ger 1963 Schuch l96l) Zbog toga se pribeglo middotkoniSeenju nekoH-ko metoda za dokazivanje virusa ~ke kalemljenje na drvenaste incHkator bHjke bullkoje reaguju specifi~nim simptomima (sejanci breskve i Prunus tomentosa) inokushylacije sokom na osetljive zeljaste biljke (Chenopodium foetidum i dr) elektronska mikroskopija i seroloske metode

bull bl1 je acpt~ na IV jugoslovenskom simpozijumu o za~titi bilja P~ 8-13 decembra IIOdlDe

38 Zastita bilia Vol 32(1) br 155 35-38 (1981) Beograd

LITERATURA

Buhl C Weidner H Zogg H (1975) Krankheiten und schadlinge an Gemiddot treide und Mais Stuttgart

Nee r g a a r d P (1977) Seed Pathology London Pitt D (1964) Studies on sharp eyespot disease of cereals Ann appl Bioi 54

77-89 We s t er u J H (1971) D)seases of Crop Plants London W i es s e M V (1977) Compendium of Wheat Diseases Minnesota

DAS AUFTRETEN DES PILZES RHIZOCTON IA SOLAN KOHN ALS URSACHE DER HALMFXULE AN WEIZEN UND GERSTpound

L Clzmtc I MHatovlc J Kilpatlc

Fakultat der Landwirtschafbswissenschaften Zagreb lnstitut ftir Pflanzenschutz

Zusammenfassung

In der Arbeit sind erste Ergebnisse tiber den Befund des Pilzes Rhizoctonia solani KUhn als Ursache der Halmfaule an Weizen und Gerste beschrieben Der Pilz wurde an diesen Getreidearten erstenmal gefestgestellt Die Determination wurde nach den Symptomen und mit Isolierung in Rcinkultur durcbgeftihrt

44 Za~tita bilja Vol 32(1) br 155 39-44 (1981) Beograd

A CONTRIBUTION TO THE KNOWLEDGE OF THE MOST IMPORTANT CAUSERS OF DISEASES OF THE SNAP BEANS ON THE TERRITORY

OF LIJEVAC POLJE

by

J ~ota

UPI OOUR Institute for Crop Fam1ing Butmir - IUlla

R Numlc

Faculty of Agriculture Sarajevo

Summary

On the basis of the published Jnvestigations of sensitivity of three sorts of snap beans (Cordon Harvester and Top Crop) to the infections with the economically most important pathogans Colletotrichum Iindemiddotbull muthianum and Pseudomonas phaseolicola in the conditions of twoshymiddotterms sowing (in spring and in summer) with and without irrigation following conclusions may be drawn

The sort Cordon was the most resistant to the infection with both pathogens because it had the lowest cllsease index with Colletotrichum lindemutlzianum whereas this index was 0 with Pseudomonas phasemiddot olicola

In relation to the infection with Colletotrichum lindemuthianmn the sort Top Crop is somewhat more resistant than the sort Harvester whereas the sensitivity to the infection with Pseudomonas phaseolicola is somewhat higher under the conditions of irrigation

The yield achived with the irrigation was regularly higher than in the culture without middotirrigation although the disease index was higher The sort HaiVester had the highest yield under the conditions without irrigation (in 1977 in the second sowing term 1428 dtha) as well as with irrigation at the same term and in the same year (1924 dtha)

ZaStita biljo Vol 32(1) br JSS SS-60 (1981) Beograd

MOojko RaDJrovlt Svetlana Vuksanovl6 lnslitut za vo6arstvo C~ak

BNliMSKI IMUNOADSORPCIONI TEST (ELISA) ZA DOKAZIVANJE VIRUSA SARKE

U radu je proveravaoa vrednost serolookog testa bullELlshySAc za dakazivanje virusa Salke uporcdo kod osetljivili i tOlerantnih sorti sljiva i kajsije Vrirus je ~eSnO dokazishyvan u UTorcima lisnih pupoljaka ovetnih pupoljaka middotkrushynicnim listicima liScu koni j zrelim plodovJilla

Uvod

U borbi protiv viroza poljopr~vrednih biljaka brza i pouzdana dishyjagnoamptika zauzima jcdno od najznacajnijJh mesta Njen zonaeaj oolazi narooito do izrazaja kod onih virusa kojd sc hrzo Sibullre putem lisnih vashy~iju i koJi zaraiavaju vise bHjnih vrsta sto je s-Jucaj sa virusom sarke za koji je utvrdeno da pored sljdve (Prunus domestica) moze zaraziti jos 23 vrste iz roda Prunus

Dijagnoza prisustva virusa ~arke bullu Mjivi se obavlja namiddotjlakSe vizuelshynim opatanjem simptoma na iScu tokom juna meseoa i na plodovima tokom leta odnosno u vreme njtihovog sazrevmja Mcdutim neke sorte ne middotispoljaVaju simptomemiddotna liScu pa ako nisu stupHe na rod simptomi sene mogu zapaa~i ~ni na plodovdma Isto taQo postJoje sorte middotkoje ce daju simptome ni na li~6u ni na plodovima Pored -toga u nekdm slu6ajemiddot vima javljaju se problcmi raspoznavanja simptoma protwrokovanih vishyrusom arke od simptoma Jinijskog mozaika (Sa v a I e oS k u amp M a coshyvei 1965 1968) in simptcgtma na plodovima tzv raquomiddotIafna Sctrkalaquo (Kegshyler et al 1964 Posnebulltmiddotte amp Ellenber-ger 1963 Schuch l96l) Zbog toga se pribeglo middotkoniSeenju nekoH-ko metoda za dokazivanje virusa ~ke kalemljenje na drvenaste incHkator bHjke bullkoje reaguju specifi~nim simptomima (sejanci breskve i Prunus tomentosa) inokushylacije sokom na osetljive zeljaste biljke (Chenopodium foetidum i dr) elektronska mikroskopija i seroloske metode

bull bl1 je acpt~ na IV jugoslovenskom simpozijumu o za~titi bilja P~ 8-13 decembra IIOdlDe

44 Za~tita bilja Vol 32(1) br 155 39-44 (1981) Beograd

A CONTRIBUTION TO THE KNOWLEDGE OF THE MOST IMPORTANT CAUSERS OF DISEASES OF THE SNAP BEANS ON THE TERRITORY

OF LIJEVAC POLJE

by

J ~ota

UPI OOUR Institute for Crop Fam1ing Butmir - IUlla

R Numlc

Faculty of Agriculture Sarajevo

Summary

On the basis of the published Jnvestigations of sensitivity of three sorts of snap beans (Cordon Harvester and Top Crop) to the infections with the economically most important pathogans Colletotrichum Iindemiddotbull muthianum and Pseudomonas phaseolicola in the conditions of twoshymiddotterms sowing (in spring and in summer) with and without irrigation following conclusions may be drawn

The sort Cordon was the most resistant to the infection with both pathogens because it had the lowest cllsease index with Colletotrichum lindemutlzianum whereas this index was 0 with Pseudomonas phasemiddot olicola

In relation to the infection with Colletotrichum lindemuthianmn the sort Top Crop is somewhat more resistant than the sort Harvester whereas the sensitivity to the infection with Pseudomonas phaseolicola is somewhat higher under the conditions of irrigation

The yield achived with the irrigation was regularly higher than in the culture without middotirrigation although the disease index was higher The sort HaiVester had the highest yield under the conditions without irrigation (in 1977 in the second sowing term 1428 dtha) as well as with irrigation at the same term and in the same year (1924 dtha)

ZaStita biljo Vol 32(1) br JSS SS-60 (1981) Beograd

MOojko RaDJrovlt Svetlana Vuksanovl6 lnslitut za vo6arstvo C~ak

BNliMSKI IMUNOADSORPCIONI TEST (ELISA) ZA DOKAZIVANJE VIRUSA SARKE

U radu je proveravaoa vrednost serolookog testa bullELlshySAc za dakazivanje virusa Salke uporcdo kod osetljivili i tOlerantnih sorti sljiva i kajsije Vrirus je ~eSnO dokazishyvan u UTorcima lisnih pupoljaka ovetnih pupoljaka middotkrushynicnim listicima liScu koni j zrelim plodovJilla

Uvod

U borbi protiv viroza poljopr~vrednih biljaka brza i pouzdana dishyjagnoamptika zauzima jcdno od najznacajnijJh mesta Njen zonaeaj oolazi narooito do izrazaja kod onih virusa kojd sc hrzo Sibullre putem lisnih vashy~iju i koJi zaraiavaju vise bHjnih vrsta sto je s-Jucaj sa virusom sarke za koji je utvrdeno da pored sljdve (Prunus domestica) moze zaraziti jos 23 vrste iz roda Prunus

Dijagnoza prisustva virusa ~arke bullu Mjivi se obavlja namiddotjlakSe vizuelshynim opatanjem simptoma na iScu tokom juna meseoa i na plodovima tokom leta odnosno u vreme njtihovog sazrevmja Mcdutim neke sorte ne middotispoljaVaju simptomemiddotna liScu pa ako nisu stupHe na rod simptomi sene mogu zapaa~i ~ni na plodovdma Isto taQo postJoje sorte middotkoje ce daju simptome ni na li~6u ni na plodovima Pored -toga u nekdm slu6ajemiddot vima javljaju se problcmi raspoznavanja simptoma protwrokovanih vishyrusom arke od simptoma Jinijskog mozaika (Sa v a I e oS k u amp M a coshyvei 1965 1968) in simptcgtma na plodovima tzv raquomiddotIafna Sctrkalaquo (Kegshyler et al 1964 Posnebulltmiddotte amp Ellenber-ger 1963 Schuch l96l) Zbog toga se pribeglo middotkoniSeenju nekoH-ko metoda za dokazivanje virusa ~ke kalemljenje na drvenaste incHkator bHjke bullkoje reaguju specifi~nim simptomima (sejanci breskve i Prunus tomentosa) inokushylacije sokom na osetljive zeljaste biljke (Chenopodium foetidum i dr) elektronska mikroskopija i seroloske metode

bull bl1 je acpt~ na IV jugoslovenskom simpozijumu o za~titi bilja P~ 8-13 decembra IIOdlDe

ZaStita biljo Vol 32(1) br JSS SS-60 (1981) Beograd

MOojko RaDJrovlt Svetlana Vuksanovl6 lnslitut za vo6arstvo C~ak

BNliMSKI IMUNOADSORPCIONI TEST (ELISA) ZA DOKAZIVANJE VIRUSA SARKE

U radu je proveravaoa vrednost serolookog testa bullELlshySAc za dakazivanje virusa Salke uporcdo kod osetljivili i tOlerantnih sorti sljiva i kajsije Vrirus je ~eSnO dokazishyvan u UTorcima lisnih pupoljaka ovetnih pupoljaka middotkrushynicnim listicima liScu koni j zrelim plodovJilla

Uvod

U borbi protiv viroza poljopr~vrednih biljaka brza i pouzdana dishyjagnoamptika zauzima jcdno od najznacajnijJh mesta Njen zonaeaj oolazi narooito do izrazaja kod onih virusa kojd sc hrzo Sibullre putem lisnih vashy~iju i koJi zaraiavaju vise bHjnih vrsta sto je s-Jucaj sa virusom sarke za koji je utvrdeno da pored sljdve (Prunus domestica) moze zaraziti jos 23 vrste iz roda Prunus

Dijagnoza prisustva virusa ~arke bullu Mjivi se obavlja namiddotjlakSe vizuelshynim opatanjem simptoma na iScu tokom juna meseoa i na plodovima tokom leta odnosno u vreme njtihovog sazrevmja Mcdutim neke sorte ne middotispoljaVaju simptomemiddotna liScu pa ako nisu stupHe na rod simptomi sene mogu zapaa~i ~ni na plodovdma Isto taQo postJoje sorte middotkoje ce daju simptome ni na li~6u ni na plodovima Pored -toga u nekdm slu6ajemiddot vima javljaju se problcmi raspoznavanja simptoma protwrokovanih vishyrusom arke od simptoma Jinijskog mozaika (Sa v a I e oS k u amp M a coshyvei 1965 1968) in simptcgtma na plodovima tzv raquomiddotIafna Sctrkalaquo (Kegshyler et al 1964 Posnebulltmiddotte amp Ellenber-ger 1963 Schuch l96l) Zbog toga se pribeglo middotkoniSeenju nekoH-ko metoda za dokazivanje virusa ~ke kalemljenje na drvenaste incHkator bHjke bullkoje reaguju specifi~nim simptomima (sejanci breskve i Prunus tomentosa) inokushylacije sokom na osetljive zeljaste biljke (Chenopodium foetidum i dr) elektronska mikroskopija i seroloske metode

bull bl1 je acpt~ na IV jugoslovenskom simpozijumu o za~titi bilja P~ 8-13 decembra IIOdlDe

60 Zastita bilja Vol 32(1) br 155 55~0 (1981) Boo11rad

ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) IN THE DETECTING SHARKA VIRUS

by

M Rankovi~ and S Vuksanovtc Fmiddotruit Research lnst~tute Ca~ak

Summary

The serologicabulll ELISA 1test nas been checked far validity in det~ ing Sharka virus iaJ susceptible and rolerant plum and apricot culbulltiv simultaneously The virus presence has been conHrmed by this testmiddot 25 Plum and 3 apricat cuJtivaTS alltifioially mfected through grafting whilth the villus presence had been previously proved on othe basis leaf symptoms or in hiossays

The virus presence has been detected in the smiddotamples prepared leaf and flower buds petals young and older leaves and from the s middotmiddot of ripe fruits Green fruits are a poor source of viruses at aU stages theilr development and are rtberefore unwitahle 131S bulltest samples

Because of the possible vtious locanzamiddottion which iiS strongly expres ed in some cultivarrs such as Opal Early Rivers and Borsum a negati reaotion does not always necessarily mean the absence of the virus The advantages of this test over the middotusage of indicator pmiddotlants are that a large number of samples oan be tested and that the results can be obtaiooo quickly

The ELISA me~hod can prove very usefmiddotul in the COtlltrol Shaltb virus when removing infected trees illl detecting latent virus carriers and for deoiding in the cases whece symptoms are indistinct

70 ZaStita h ilia Vol 32(1) br 155 61-70 (1981) BeQSirad

M c Leo d H A and Roit c e y W R (1973) Analytical methods for pesticide residues in foods National Health and Welfare Health Protection Brauch Canada

R it c e y W R Sa vary G and M c C u II y K A (1972) Organochlorine insecti shycide residues in human milk evaporated milk and some milk substlimiddottutes in Canada J Pmiddotubl Hlth 63 125-132

Savemi komitet 7a poljopr~vrcdu (1980) Pcsticidi u prometu u Jugoslaviji Prishyvredni pregled Beograd

To do ro v D Yo to v Y and S erg c y eva D (1974) Elimination and retenshytion of some pesticides in white brine cheese and elow sheese in the proces-sshymiddoting of milk Vet Med Nauki 11 95-100

T y I 1 in en H K a r p p a n en E and N u r m i E (1975) Chlorina-ted hydrocarshybons m beef oow liwer and mHk products and total mercury in cow Jiwer J Scient Agric Soc of Finland 47 533-540

V o j i nov i c V and K 1 j a j i c R (1978) Pesticide residues levels in foodstuffs in Yugo~lavia 1971-1977 IV International congress of pesticide chemistry

Ztirich V o jJ nov i c V Vito ro vic S and N cS k o vic N (1973) Ostaoi insekticida middot iz grupe hlorovanih ugljovodonika u mleku i mlecnim proizvodima tokom

godine Hrana i middotishrana 16 411-416 Yamada T and Saka moto Y (1973) Survey of pesticide residues in vegeshy

tables fruits cows milk fish and shellfish Hiroshima Prefect lnst Prevenshytion Bnviron Pollution Annu Rep 47 54-56

P r a v i I n i k o i1meni pravlilnika o maksima1no dozvoljen1m kolicinama pesticida u Zivotnim narnirnicama Sl list SFRJ 17 442-446 1976

P r a v oi 1 n j k o kolicinama pesticida i drugih otrovnih m atenija hormona i antibioshytika koji -se mogu nalaztti u zivotnim namimicama Sl list SFRJ 20 910-924 1980

INVESTIGATIONS OF PESTICIDE RESIDUE LEVELS IN FOODSTUFFS IN SERBIA (1979-1980)

1 Perle B Ram1elovic V Vojinovic 1 N Neiikovic INEP Department of postioides Banatska 31-b Zemun and

The Ag ricultural Comhinc raquoDcliSeslaquo Vladicin Han

Summa r y

The monitoring pmgram for peampticide residue levebullls middotin foodsmiddottuffs of plant and animal origin cover the peri-od from 1979 to 1980T_he somples were collected jn middotthe retail netWIOIk from difforent locahtues (towns) in Serbia

The organochlorine and orgamophosphate res1dues were detenninshyed by gas-liquid chromat01gt1aphy Results have shown that bullthere are pesticide residues from bullthe group of chlorinabullted hydrocarbons oin most of the controlled products At thabullt the residues occuring most frequently are thooe of a-HCH arul linmiddotdane The estabtished amounts were 1n most cases quite small Hoshywever in a certain although small number of samples the established amounts were greater than the maximmiddotum allowed Qy law

70 ZaStita h ilia Vol 32(1) br 155 61-70 (1981) BeQSirad

M c Leo d H A and Roit c e y W R (1973) Analytical methods for pesticide residues in foods National Health and Welfare Health Protection Brauch Canada

R it c e y W R Sa vary G and M c C u II y K A (1972) Organochlorine insecti shycide residues in human milk evaporated milk and some milk substlimiddottutes in Canada J Pmiddotubl Hlth 63 125-132

Savemi komitet 7a poljopr~vrcdu (1980) Pcsticidi u prometu u Jugoslaviji Prishyvredni pregled Beograd

To do ro v D Yo to v Y and S erg c y eva D (1974) Elimination and retenshytion of some pesticides in white brine cheese and elow sheese in the proces-sshymiddoting of milk Vet Med Nauki 11 95-100

T y I 1 in en H K a r p p a n en E and N u r m i E (1975) Chlorina-ted hydrocarshybons m beef oow liwer and mHk products and total mercury in cow Jiwer J Scient Agric Soc of Finland 47 533-540

V o j i nov i c V and K 1 j a j i c R (1978) Pesticide residues levels in foodstuffs in Yugo~lavia 1971-1977 IV International congress of pesticide chemistry

Ztirich V o jJ nov i c V Vito ro vic S and N cS k o vic N (1973) Ostaoi insekticida middot iz grupe hlorovanih ugljovodonika u mleku i mlecnim proizvodima tokom

godine Hrana i middotishrana 16 411-416 Yamada T and Saka moto Y (1973) Survey of pesticide residues in vegeshy

tables fruits cows milk fish and shellfish Hiroshima Prefect lnst Prevenshytion Bnviron Pollution Annu Rep 47 54-56

P r a v i I n i k o i1meni pravlilnika o maksima1no dozvoljen1m kolicinama pesticida u Zivotnim narnirnicama Sl list SFRJ 17 442-446 1976

P r a v oi 1 n j k o kolicinama pesticida i drugih otrovnih m atenija hormona i antibioshytika koji -se mogu nalaztti u zivotnim namimicama Sl list SFRJ 20 910-924 1980

INVESTIGATIONS OF PESTICIDE RESIDUE LEVELS IN FOODSTUFFS IN SERBIA (1979-1980)

1 Perle B Ram1elovic V Vojinovic 1 N Neiikovic INEP Department of postioides Banatska 31-b Zemun and

The Ag ricultural Comhinc raquoDcliSeslaquo Vladicin Han

Summa r y

The monitoring pmgram for peampticide residue levebullls middotin foodsmiddottuffs of plant and animal origin cover the peri-od from 1979 to 1980T_he somples were collected jn middotthe retail netWIOIk from difforent locahtues (towns) in Serbia

The organochlorine and orgamophosphate res1dues were detenninshyed by gas-liquid chromat01gt1aphy Results have shown that bullthere are pesticide residues from bullthe group of chlorinabullted hydrocarbons oin most of the controlled products At thabullt the residues occuring most frequently are thooe of a-HCH arul linmiddotdane The estabtished amounts were 1n most cases quite small Hoshywever in a certain although small number of samples the established amounts were greater than the maximmiddotum allowed Qy law

78 Zaamptita bHia Vol 32(1) br 155 71-78 (1981) Beograd

EFFECT OF HERBICIDES ON THE GROWTH RATE AND YIELD OF MAIZE INBRED LINES

L MrilnskimiddotStefanovlc Maize Resarch Institute Zemun Poljc

Beograd-Zemun

Two year rczults of investigation the effect of herbicides on the growth rate and yield of five maize inbred lines are presented in this paper Five herbicide mixtures were used each in three doses The bight of treated and untreated plants was measured during the growing season and the grain yield after harvesting

On the basis of results obtained it was concluded that each inbred reacted in a specific way and the alach1or did not cause a reduction in the growth rate and yield of middotinbred lines even if applied with a high dosis (D3) Inbred R-59 was found susceptible to this herbicide Metolachlor applied with a dosis of 9 1ha affected unfavourably the growth rate and yield of investmiddotigated inbrcds

Eradican and cianazin caused a stronger reaction at the beginning of the growing season R-319 was found as the most susceptible inbred to the herbicides Stomp caused more or less a reduction in the growth rate and yield of investigated inbreds depending on the dosis applied As the most susceptible inbreds to these herbicides Inbrcds V-158 and W-64 A were singled out

Za~tita bilja Vol 32(1) br 155 79-90 (1981) Beograd 89

LITERATURA

But in H Morphologischc und taxonomische Untersuchungen an Naemacyclus nivcus (Pers ex Fr) Fuck ex Sacc und verwandten Alten Eur J For Path 3 146-163 1973

D a r k e r G D The Hypodermataceac of conifers Contrib Amold Arboretum Harvard Univ 1 1-131 1932

Gad g i I P D How Important Is Naem acyclus Whats new in Forest research No 56 1977 Rotorua

Gibson I A S Diseases of Forest Trees Widely Planted as Exo tmiddotics in the Troshypics and Southern Hemisphere Part II Genus Pinus Com For Ins Oxmiddot ford 1979

G r e m m e n J Fungi colonizing living and dead tissue of Pinus sylvestris and P nigra Kew Bulletin Vol 31 (3) 1976

K a is A G Technique for inocuJating Pi-ne seedlings with Soirrhia acicola Proc of The Amer Pytopath Society Volume I 1974 middot

K a r adz i c D Infection of Scotch Pine by Naemacyclus minor The Inter conmiddot ference IUFR9 - Needle diseases Sarajevo 1980

Kistler B R and Merrill W Etiology Symptomology and Control of Naemiddot macyclus Needlecast of Scotch Pine Phybulltopathology Vol 68 267-271 1978

Magnani G Sulla presenza di Naemacyclus 11iveus su aghi dbulli Pinus radiata Centro Sper Agric e Forestate ll 1972 p 315-320

M iII a r C S Role of Lophodermella species in Premature Death of Pine needles in Scotland Report on Forest Research 1970

M i 11 a r C S and Minter D W Naemacyclus minor CMI Descriptions of Pathogenic Fungi and Bacteria No 659 1980

CONTRIBUTION TO THE KNOWLEDGE ON THE BIOLOGY OF THE FUNGI NAEMACYCLUS MINOR BUTIN - CAUSER OF NEEDLECAST OF SCOTS PINE

D Karadllc Fores1ry Faculty Beograd

R Zorlc SIK raquoPancevoo - Forestry service Kovin

Summary

Naemacyclus minor is an Ascomyceteous fungi which cause needle b1ight in pine species Some authors consider this fungi as the causal organism of a needlecast but others describe it as saprophytic Isolashytions N minor from newly developed 1-moold needles in the absence of any other known pathogens shows that N minor can be harmful to Scots pine and the premature needle casting is associated by this fungus

I

90 Za~titamp obilia Vol 32(1) br lSS 7~90 (1981 Beograd

The fungus described corresponds most nearly to the description o N minor given by But in (1973) The optimum temperature for myshycelial growth is around 2sbullc and for ascocarp production in culture 18-20C The most favourable media for ascocarp formation are OatshymeaJ and Dry - needle agars On these media ascocarps formed from 11 - zsbullc Growth was fastest on potato - carrot agar but strongest on malt agar N minor showed cultural variations into two main types which was most evident on malt agar Artificial inoculations 2- years old seedlings Scots pine (with a water suspension of ascospores from 5 weeks old cultures of N minor on 2 malt agar) have shown that N minor can infect Scots pine needles under controlled conditions and proves the pathogenicity of this fungi

N minor is widely distributed in Serbia in Scots pine plantations aged between 10-20 years

99 Zastita bilja V-ol 321) br 155 91-99 (1981) Boo~rod

INTENSITY OF THE ATTACK OF LOPHODERMIUM SPECIES AND THE DEVELOPMENT OF THE DISEASE ON SOME YUGOSLAV PROVENANCES

OF SCOTS PINE SEERLIINGS

V Lazarev Facultu of Forestry Sarajevo

Summary

In previous investigations regarding the susceptibility of Scots pine provenances to attack of Lophodermium pinastri (Schrad) Chev various facts are considered as relevant However the nature of the plant resismiddot tant is not entirely understood

Until now variability of Lophodermium species is not taken under consideration This could cause some difficulties in controling the diseshyases and in the discovering resistant pine provenances

Accepting the new approach of taxonomy of Lophodermium spp with more species colonizing Scots pine needles we have investigated the intesity of attack and development of the disease on one year old seedlings of some Yugoslav provenances of Scots pine The seedlings were grown on natural and on artificial supstratum under noncontroled and partly controled condiHons for infection

It was found that there are two Lophodermium species colonizing pine needles in Bosnia with different bioecological characteristics These are L pinastri and L seditiosum spec nov

Under noncontroled condition for infection L seditiosum cause severe damage on young primary needles while L pinastri attacks only old primary needles (oldest primary needles - coleptiles) and has no economic importance in nurser~es

Under partly controled conditions L Seditiosum attacs both youngand old primary needles middot

In our ~investigations it was olso shown that interaction between plant provenances and ecological conditions of sites are most important tactor for intensity of the disease Geographical variability of Scots pine and ecological characteristics of experimental plots are olso conmiddot sidered as a relevant for the differences in the dntensity of Lophodermium attack and developm~nt of the disease

Za~tita bilja Vol 32(1) br 155 79-90 (1981) Beograd 89

LITERATURA

But in H Morphologischc und taxonomische Untersuchungen an Naemacyclus nivcus (Pers ex Fr) Fuck ex Sacc und verwandten Alten Eur J For Path 3 146-163 1973

D a r k e r G D The Hypodermataceac of conifers Contrib Amold Arboretum Harvard Univ 1 1-131 1932

Gad g i I P D How Important Is Naem acyclus Whats new in Forest research No 56 1977 Rotorua

Gibson I A S Diseases of Forest Trees Widely Planted as Exo tmiddotics in the Troshypics and Southern Hemisphere Part II Genus Pinus Com For Ins Oxmiddot ford 1979

G r e m m e n J Fungi colonizing living and dead tissue of Pinus sylvestris and P nigra Kew Bulletin Vol 31 (3) 1976

K a is A G Technique for inocuJating Pi-ne seedlings with Soirrhia acicola Proc of The Amer Pytopath Society Volume I 1974 middot

K a r adz i c D Infection of Scotch Pine by Naemacyclus minor The Inter conmiddot ference IUFR9 - Needle diseases Sarajevo 1980

Kistler B R and Merrill W Etiology Symptomology and Control of Naemiddot macyclus Needlecast of Scotch Pine Phybulltopathology Vol 68 267-271 1978

Magnani G Sulla presenza di Naemacyclus 11iveus su aghi dbulli Pinus radiata Centro Sper Agric e Forestate ll 1972 p 315-320

M iII a r C S Role of Lophodermella species in Premature Death of Pine needles in Scotland Report on Forest Research 1970

M i 11 a r C S and Minter D W Naemacyclus minor CMI Descriptions of Pathogenic Fungi and Bacteria No 659 1980

CONTRIBUTION TO THE KNOWLEDGE ON THE BIOLOGY OF THE FUNGI NAEMACYCLUS MINOR BUTIN - CAUSER OF NEEDLECAST OF SCOTS PINE

D Karadllc Fores1ry Faculty Beograd

R Zorlc SIK raquoPancevoo - Forestry service Kovin

Summary

Naemacyclus minor is an Ascomyceteous fungi which cause needle b1ight in pine species Some authors consider this fungi as the causal organism of a needlecast but others describe it as saprophytic Isolashytions N minor from newly developed 1-moold needles in the absence of any other known pathogens shows that N minor can be harmful to Scots pine and the premature needle casting is associated by this fungus

I

90 Za~titamp obilia Vol 32(1) br lSS 7~90 (1981 Beograd

The fungus described corresponds most nearly to the description o N minor given by But in (1973) The optimum temperature for myshycelial growth is around 2sbullc and for ascocarp production in culture 18-20C The most favourable media for ascocarp formation are OatshymeaJ and Dry - needle agars On these media ascocarps formed from 11 - zsbullc Growth was fastest on potato - carrot agar but strongest on malt agar N minor showed cultural variations into two main types which was most evident on malt agar Artificial inoculations 2- years old seedlings Scots pine (with a water suspension of ascospores from 5 weeks old cultures of N minor on 2 malt agar) have shown that N minor can infect Scots pine needles under controlled conditions and proves the pathogenicity of this fungi

N minor is widely distributed in Serbia in Scots pine plantations aged between 10-20 years

99 Zastita bilja V-ol 321) br 155 91-99 (1981) Boo~rod

INTENSITY OF THE ATTACK OF LOPHODERMIUM SPECIES AND THE DEVELOPMENT OF THE DISEASE ON SOME YUGOSLAV PROVENANCES

OF SCOTS PINE SEERLIINGS

V Lazarev Facultu of Forestry Sarajevo

Summary

In previous investigations regarding the susceptibility of Scots pine provenances to attack of Lophodermium pinastri (Schrad) Chev various facts are considered as relevant However the nature of the plant resismiddot tant is not entirely understood

Until now variability of Lophodermium species is not taken under consideration This could cause some difficulties in controling the diseshyases and in the discovering resistant pine provenances

Accepting the new approach of taxonomy of Lophodermium spp with more species colonizing Scots pine needles we have investigated the intesity of attack and development of the disease on one year old seedlings of some Yugoslav provenances of Scots pine The seedlings were grown on natural and on artificial supstratum under noncontroled and partly controled condiHons for infection

It was found that there are two Lophodermium species colonizing pine needles in Bosnia with different bioecological characteristics These are L pinastri and L seditiosum spec nov

Under noncontroled condition for infection L seditiosum cause severe damage on young primary needles while L pinastri attacks only old primary needles (oldest primary needles - coleptiles) and has no economic importance in nurser~es

Under partly controled conditions L Seditiosum attacs both youngand old primary needles middot

In our ~investigations it was olso shown that interaction between plant provenances and ecological conditions of sites are most important tactor for intensity of the disease Geographical variability of Scots pine and ecological characteristics of experimental plots are olso conmiddot sidered as a relevant for the differences in the dntensity of Lophodermium attack and developm~nt of the disease

90 Za~titamp obilia Vol 32(1) br lSS 7~90 (1981 Beograd

The fungus described corresponds most nearly to the description o N minor given by But in (1973) The optimum temperature for myshycelial growth is around 2sbullc and for ascocarp production in culture 18-20C The most favourable media for ascocarp formation are OatshymeaJ and Dry - needle agars On these media ascocarps formed from 11 - zsbullc Growth was fastest on potato - carrot agar but strongest on malt agar N minor showed cultural variations into two main types which was most evident on malt agar Artificial inoculations 2- years old seedlings Scots pine (with a water suspension of ascospores from 5 weeks old cultures of N minor on 2 malt agar) have shown that N minor can infect Scots pine needles under controlled conditions and proves the pathogenicity of this fungi

N minor is widely distributed in Serbia in Scots pine plantations aged between 10-20 years

99 Zastita bilja V-ol 321) br 155 91-99 (1981) Boo~rod

INTENSITY OF THE ATTACK OF LOPHODERMIUM SPECIES AND THE DEVELOPMENT OF THE DISEASE ON SOME YUGOSLAV PROVENANCES

OF SCOTS PINE SEERLIINGS

V Lazarev Facultu of Forestry Sarajevo

Summary

In previous investigations regarding the susceptibility of Scots pine provenances to attack of Lophodermium pinastri (Schrad) Chev various facts are considered as relevant However the nature of the plant resismiddot tant is not entirely understood

Until now variability of Lophodermium species is not taken under consideration This could cause some difficulties in controling the diseshyases and in the discovering resistant pine provenances

Accepting the new approach of taxonomy of Lophodermium spp with more species colonizing Scots pine needles we have investigated the intesity of attack and development of the disease on one year old seedlings of some Yugoslav provenances of Scots pine The seedlings were grown on natural and on artificial supstratum under noncontroled and partly controled condiHons for infection

It was found that there are two Lophodermium species colonizing pine needles in Bosnia with different bioecological characteristics These are L pinastri and L seditiosum spec nov

Under noncontroled condition for infection L seditiosum cause severe damage on young primary needles while L pinastri attacks only old primary needles (oldest primary needles - coleptiles) and has no economic importance in nurser~es

Under partly controled conditions L Seditiosum attacs both youngand old primary needles middot

In our ~investigations it was olso shown that interaction between plant provenances and ecological conditions of sites are most important tactor for intensity of the disease Geographical variability of Scots pine and ecological characteristics of experimental plots are olso conmiddot sidered as a relevant for the differences in the dntensity of Lophodermium attack and developm~nt of the disease

99 Zastita bilja V-ol 321) br 155 91-99 (1981) Boo~rod

INTENSITY OF THE ATTACK OF LOPHODERMIUM SPECIES AND THE DEVELOPMENT OF THE DISEASE ON SOME YUGOSLAV PROVENANCES

OF SCOTS PINE SEERLIINGS

V Lazarev Facultu of Forestry Sarajevo

Summary

In previous investigations regarding the susceptibility of Scots pine provenances to attack of Lophodermium pinastri (Schrad) Chev various facts are considered as relevant However the nature of the plant resismiddot tant is not entirely understood

Until now variability of Lophodermium species is not taken under consideration This could cause some difficulties in controling the diseshyases and in the discovering resistant pine provenances

Accepting the new approach of taxonomy of Lophodermium spp with more species colonizing Scots pine needles we have investigated the intesity of attack and development of the disease on one year old seedlings of some Yugoslav provenances of Scots pine The seedlings were grown on natural and on artificial supstratum under noncontroled and partly controled condiHons for infection

It was found that there are two Lophodermium species colonizing pine needles in Bosnia with different bioecological characteristics These are L pinastri and L seditiosum spec nov

Under noncontroled condition for infection L seditiosum cause severe damage on young primary needles while L pinastri attacks only old primary needles (oldest primary needles - coleptiles) and has no economic importance in nurser~es

Under partly controled conditions L Seditiosum attacs both youngand old primary needles middot

In our ~investigations it was olso shown that interaction between plant provenances and ecological conditions of sites are most important tactor for intensity of the disease Geographical variability of Scots pine and ecological characteristics of experimental plots are olso conmiddot sidered as a relevant for the differences in the dntensity of Lophodermium attack and developm~nt of the disease