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. . , . . , . , . . , . . , . . , . . ESCHERICHIACOLIC600 pKEN, GFP
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.. - , 12 - IN VIVO ..
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53
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55
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56
. . , . . CRAMBE KOKTEBELICA (JUNGE) N. BUSCH CRAMBE MITRIDATIS JUZ....
57
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59
. . , . . 2, . 1 ....
60
4.
. . , . . , . . Trigonella foenum-graecum L.
62
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5
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63
. . delF508 CFTR .
65
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66
. . , . . , . . , . . - - .
67
. Goloborodko, V. Konopelnyuk, L. Ostapchenko EFFECT OF POWDER FROM TRIGONELLA FOENUM-GRAECUM SEED ON BODY WEIGHT OF RATS UNDER CONDITIONS OF HIGH CALORIE DIET.
69
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70
. . - ..
72
. ., . . - ..
73
. . , . . , . . - TENEBRIO MOLITOR
75
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76
5.
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78
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79
6.
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81
. . ...
82
. . , . . - ACORUS CALAMUS L. .
84
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- 2015 86 . . ... 88 . . , ..
89
. . , . . C .. 90 . . , . . 2015
92
. . , . . ( , -.)...
95
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96
. . , . . PHRAGMITES AUSTRALIS (CAV.) TRIN. EX STEUD...
98
. ., . . , . . .. 100 . . , . .
102
. . , . . . 104 . .
105
. . ..
107
. . , . . CERATOPHYLLUM DEMERSUM L. .
109
7.
. . , . . (. ).. 112 . . , . . ZIZYPHUS JUJUBA MILL., . ..
113
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115
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117
. . - REBUTIA (CACTACEAE)...
118
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120
. . , . . LOPHANTHUS ANISATUS ADANS
122
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124
. . SERPYLLUM (MILL.) DUBY THYMUS L. (LAMIACEAE) ...
125
. . - .
128
. . - ERODIUM CICUTARIUM (L.) L'HER. ..
130
. . , . . HYBISCUS ESCULENTUS L. (MALVACEAE)
131
. . ...
133
. . - RHODODENDRON SUBSECT. RHODODENDRON (ERICACEAE)
134
. . , . . CAMPYLOPUS INTROFLEXUS (HEDW.) BRID. , ( ).
136
. . , . . SANSEVIERIA GRANDICUSPIS HAW. (ASPARAGACEAE)..
138
. . , . . -
139
. . - STIPA CAPILLATA L.(POACEAE) .
141
. . , . . , . . VRN-A1 ...
142
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8. ,
. . , . . , . . (DAPHNIA) (, ) . 144 . . , . . (CHRYSOMELIDAE) .. 146 . . ...
148
. . , . . (MYOCASTOR COYPUS) 150 . . , . . (HIRUNDO RUSTICA) ...
152
. . , . . -
154
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157
. . , . . (OLIGOCHAETA, LUMBRICIDAE) ..
158
. . , . . - (HYMENOPTERA, BRACONIDAE: APHIDIINAE) (APHIDIDAE) (CENTAUREA, ASTERACEAE)
159
. . , . . DICHOGASTER BOLAUI (MICHAELSEN, 1891) : -...
161
. . , . . , . . , . . , . . (BIVALVIA) . : , , .
163 . . , . .
165
. . , . . , ...
166
. . ASTACUS PACHYPUS (CRUSTACEA, DECAPODA, ASTACIDAE)
167
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9
. . APORRECTODEA DUBIOSA (RLEY, 1881)..
168
. . , . . - (LYRURUS TETRIX L.) .
170
. . , . .
172
. . FAGOTIA ACICULARIS FAGOTIA ESPERI ..
174
. . (AMPHIBIA, ANURA)
175
. . , . . , . . (MOLLUSCA, GASTROPODA, PULMONATA, BULINIDAE).
177
. . (ACARIFORMES, ASTIGMATA) .
179
. . , . . , . . (ACARIFORMES, ACAROIDEA) .
180
. . ,. . , . .
182
. . , . . DREISSENA POLYMORPHA (MOLLUSCA: BIVALVIA:DREISSENIDAE)
183
. . (PHALACROCORAX CARBO) ( )..
184
. ., . . . ..
187
. . , ..
THEODOXUS FLUVIATILIS ... 188
. . , .. ..
189
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191
9.
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193
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10
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194
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196
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197
10.
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202
. . . ..
204
. . , . . - ..
206
. . , . . SPIRAEA L., .
207
. . , . . , . . . .. ..
209
. . , . . PAEONIA L
211
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213
.. ... 214
11. -
. B. , . .
217
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249
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250
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. . , . . .. 252 . . , . . 254 . . , . . 256 . . , . . : ... 258 . . , . . 260 . . , . . ..
262
. . , . ..................................... 263 . . , . . , A,
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278
. . , . . . 280 . . , . . ...... 282 . . , . . ... 283 . . , . . ... 285 . . , . . . 286 . . , . . ... 287 .. . 289 . . , . .
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. 290 . . , . . (-1200)... 292 . . , . . ?............................................................... 294 . . , . . .
295
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. . -.. 312 . . , . . , . . , . ..
314
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ERYSIPELOTHRIX RHUSIOPATHIAE 317 . . .
319
. . , . . PSEUDOMONAS, ...
320
. . LACTOBACILLUS ...
321
. . , . . ALCALIGENES FAECALIS 452 -....
322
. . , . . , . . , . . , . . , , ERWINIA CAROTOVORA ORCHID................... 323 . . BACILLUS MEGATERIUM 500...
325 . . , . . , . . , . . , . . - .
326
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328
15.
. . , . . .
331
. . , . . BETULA OBSCURA (A. Kotula) BETULA PENDULA (Roth.) .
333
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335
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336
. . (), . . , . . ...
338
. . ., . . (2012-2015 .)..
339
. .
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15
..
341
. .
343
. . ., . . , . . ...
344
. . : -
346
. . Co, Cu, Mn, Zn
348
. . LONICERA TATARICA L. .. 350 . . , . . , . . , . . HUPERZIA SELAGO (HUPERZIACEAE) -....
352
. . , . . , . . - .
354
. . , . . ...
356
. . , . . , . . , . . - BIDENS FRONDOSA L. . 358 . . , . . OXYTREE ... 360 . , . . , . . , . . . . ...
361
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364
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367
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370
. . - ..
372
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373
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375
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377
16.
. . '
379
. . ., . . ., . . -
381
. . , . . ..
382
. . , . .
384
. . , . . , . . RUBUS CAESIUS L.
386
. . , . . - ( - ) ..
387
. . , . . ..
389
. . , . . PINUS SYLVESTRIS L..
392
. . , . . (Brachydanio rerio, Hamilton 1822)
394
. . , . . - . -
396
17.
. . , . . I LYMNAEA S.STR. (GASTROPODA: PULMONATA: LYMNAEIDAE) ...
399
. . , . . (MOLLUSCA, PULMONATA, PLANORBINAE) ..
401
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2016
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612.76.612.825
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delt, br. , , . , , . , , , post-hoc . .
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2. . . / . . , . . // .. . 1993. . 68, 6. . 57.
3. . . / . . , . . , . . // . 1998. 4. . 3-13.
4. Akerboom T. Glutathione: metabolism and physiological functions / . Akerboom, . Sies, Ed. J. Vina. Boston: GRG Press, 1990. P. 152.
5. Filaire E. Lipid peroxidation and antioxidant status in rat: effect of food restriction and wheel running / . Filaire, . Rouveix, . Massart // Eur. J. Appl. Physiol. 2009. 107(2). P. 243-250.
6. Hayes S. D. Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defense against oxidative stress / S. D. Hayes, L. I. McLellan // Free Radical. Res. 1989. V. 31. P. 273-300.
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: 1. . 76922 , 1 02F 1/24. /
. ., . ., . ., . ., . ., . .; : . . . u201207123; . 12.06.2012; . 25.01.13., . 2.
2. . 90119 , 1 02F 1/24. / . ., . ., . ., . ., . ., . ., . .; : .. . u201315133; . 24.12.2013; . 12.05.14., . 9.
3. Pseudomonas / . . , . . , . . [ .] // . 2013, 4. . 7280.
4. . . : / . . . : , 2009. 102 .
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, Agilent 7890 (Agilent Technologies, USA) RTSBA6 6.2 MIDI Sherlock, Microbacterium sp. -3 Sim Index = 0,942 Microbacterium barkeri (Aureobacterium, Corynebacterium). : 12- 15:0 nteiso (46,4%), 14- 17:0 nteiso (27,0%) 14- 16:0 iso (16,9%) . 12-
-
46
P. maltophilia ONU329, 2,7 17,4%. 17:0 iso (1,7% ) . , 15- (17:0 iso) 14- (17:0 nteiso) P. maltophilia ONU329 [1]. Pseudomonas [17:0 nteiso/ 17:0 iso] [15:0 nteiso/ 17:0 nteiso], , Microbacterium spp. Pseudomonas spp. M. barkeri -3 P. maltophilia ONU329 so nteiso , 18:0 iso (0,06%) 19:0 anteiso (0,03%).
M. barkeri -3 , P. maltophilia ONU 329, : (10:0) (12:0) - - 15:0. M. barkeri -3 P. maltophilia ONU 329 , , (0,08%), (17:0). , 2- 3- . : 16:1 2OH (0,05%), 16:0 iso 3OH (0,08%), 17:0 2OH (0,11%), 17:0 3OH (0,05%), 18:1 2OH (0,16%), (0,08-0,10%) (0,35-0,40%). , P. maltophilia ONU329 , 7,4% 10 13.
, , . , Microbacterium barkeri -3. , ( ) . - M. barkeri -3 P. maltophilia ONU 329 .
1. Pseudomonas /
. . , . . , . . [ .] // . 2014. 3(27). C. 3140.
2. Pseudomonas / . . , . . , . . [ .] // . 2013, 4. . 7280.
579.222
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-
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-
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. 27,1 / 3, 0,6 / 3 .
, , . 20178 / , 25759 / .
60 , . 30 . , . .
. 245,7 3 / , 240 3 / .
, . 11,7%. 5%.
. .
: 1. Hill D. T. Simplified Monod kinetics of methane digestion of animal wastes / D. T. Hill //
Agricultural Wastes. - 1983. - 5. - P. 1-16. 2. Mazur R. Poultry manure as a substrate for methane fermentation: problems and solutions / R.
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577.2: 579.2
ESCHERICHIACOLIC600 pKEN, GFP
. . , . . , . , . . , . . , . . ,
. . .. , . , 2, 65082 ,
GFP (greenfluorescentprotein), , , . Aequoreavictoria. 395 510 [1].
EscherichiacoliC600 pKEN, GFP.
. EscherichiacolipKEN 1 12 000 / 5 . 500 1 (25 -HCl, 10 , 50 , 8,0). 200 2 (0,1 NaOH, 1%SDS). 3 .
150 3 (3 , 4,5) 10 . 10 12 000 /. 96 10 12 000 /.
-
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70 . 50 .
EscherichiacoliC600 . 37 18 . LB 37 (150 /). 5 12 000 /. , 1 0,1 CaCl2 () 30 . . 50 1 . 60 42 . 800 LB 1,5 . LB 25 / . 37 .
E.coliC600, GFP, () -. 37 LB, - . 37. 96 15 . 900 . (0,5% ) (900).
E.coliC600 pKEN. GFP . .
( ) - . , , , -. . . - , . , .
, , , [2]. - [3]. .
: 1. Morise J.G. Intermolecularenergytransferinthebioluminescent system of Aequorea / J. G Morise,
O. Shimomura, F. H. Johnson, J. Winant // Biochemistry. 1974. Vol. 13. P. 2656 2662. 2. Nautiyal C. S. Environmental Escherichia coli occur as natural plant growth-promoting soil
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-
51
581.1 , 12 -
IN VIVO . . . - , . , 148, . , 03143
.
80- . . , () . 12 - Synechocystis sp. 6803. , , [1,2].
, desA (12) 35s (), licBM3 [3]. Nicotiana tabacum Agrobacterium- . [4]. -. .
(0 20 , -5 80). . Nicotiana tabacum Nicotiana tabacum, gfp:licBM3[5]. , in vitro in vivo. .
in vivo (5-7 , -) . 4-5 , - (0 20 , -5 80). ().
, - ( , , .). . , desA . , . , [6]. , 12 - . .
1. . . , / .
// . 2001. 41 . 163-198. 2. Guschina I. A. Mechanisms of temperature adaptation in poilkilotherms / I. A Guschina,
J. L. Harwood // FEBS Letters. 2006. 580. . 5477 5483.
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52
3. . . , Clostridium thermocellum, - / . . , . . , . . // . 2002. .36. 4. . 868-876.
4. . . , 9 12 / . , . , . . , . . , . . , . . , . . , . . - // . 2010. . 14. 1 . 127-133.
5. Gerasymenko I. M. Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial 9 or 12 acyllipid desaturases and thermostable lichenase / L. O. Sakhno, T. . Kyrpa, A. N. Ostapchuk, T. A. Khadjiev, I. V. Goldenkova-Pavlova, Y. V. Sheludko // Russian J. Plant Physiol. 62(3), 283-291.
6. Beyer W.F. Assaying for superoxiddismutase activity some large conseguences of minor changes in conditions / W. F.Beyer, I. Fridovich // Anal. Biochem. V. 161, N2. 1987. P.559- 566 637.146
. . , . . , . . , . .
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: VIVO ( -); Good Food ( BIOCHEM srl.), ( ). 1.
. 2,5%.
-
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,
1 VIVO () Lactococcus lactis subsp. lactis; Lactococcus lactis subsp. diacetylactis; Lactobacillus acidophilus; Lactobacillus delbrueckii subsp. bulgaricus; Lactobacillus casei; Acetobacter aceti; Streptococcus salivarius subsp. thermophilus; Saccharomyces unisporus
2 Good Food ()
Leuconostoc mesenteroides subsp. cremoris; Lactococcus lactis subsp. cremoris; Lactobacillus lactis subsp. lactis; Lactococcus lactis subsp. lactis biovar. diacetylactis; Lactobacillus acidophilus; Lactobacillus delbrueckii subsp. bulgaricus; Saccharomyces cerevisiae
3 ()
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.
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1. . . - . / . . ,
. . , . . . , 2011. 136. 2. . .
/ . . , . . // : . . .: , 2015. 4. .93-98.
3. // . 2006. 5(8). .8-11.
4. . . , - / . . , . . // : . . .: , 2014. 2. .64-70.
576.858.9
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-
57
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, , , Spirulina platensis. 0,74 /3, . .
1. Shantanu W. Nutrient utilization from anaerobic digester effluent through algae cultivation:
dissertation doctor of philosophy in Biological Engineering / Wahal Shantanu. - Utah, 2010.-111 2. Microalgae Cultivation: Nutrient Recovery from Digestate for Producing Algae Biomass /
D. Verones, A. Id, G. DImporzano, F. Adani. // Associazione Italiana Di Ingegneria Chimica. 2015. 43. . 120126.
3. Singh M. Microalgal system for treatment of effluent from poultry litter anaerobic digestion / M. Singh, D. L Reynolds, K. C Das. // Bioresource Technology. 2011. . 102.
606:57.085:582.683 + 574
CRAMBE
KOKTEBELICA (JUNGE) N. BUSCH CRAMBE MITRIDATIS JUZ. . . , . . , .
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Crambe koktebelica (Junge) N. Busch Crambe mitridatis Juz., (Cr. koktebelica) (Cr. mitridatis). , [3, 4]. , -. , .
-
58
[5]. .
in vitro . , - .. (C. koktebelica - , , 2013 , C. mitridatis , . , 2013 ). , . 70% 60 , 3 . 5 MS 16- +24 [6].
MS/2 - (16- +24).
( ) , ( ). , , , . , C. Mitridatis (79%) C. Koktebelica (33%). C. Koktebelica ( 50%) C. Mitridatis ( 16,7%) [7].
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-
59
1. ..
/ . . // . 2010. . 44, 3. . 58-72. 2. .. . / . . , . .
.: , 2005. 269. 3. .., .. // . .
.: , 2009. . 360. 4. .., .. // . .
.: , 2009. . 362. 5. .., .., .. Crambe
koktebelica Crambe mitridatis (BRASSICACEAE) / . . // 2014 - . 71, 2. - . 188 195.
6. Murashige T. A revised medium for rapid growth and bioassays with tobacco tissue cultures / T. Murashige, F. Skoog // Physiol. Plant. 1962. V, N 15. P. 473-497.
7. .., .., .. in vitro , / .. // 2015. . 17, . 241 244.
579.222.2
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2. . . , / . , . . , . . , . . // . . - 2010. .5. - 4.- .25-31.
3. . . / . . , . . , . . , . , . . , . , . , . // . 2013. . 29. . 5560.
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http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I21DBN=UJRN&P21DBN=UJRN&S21STN=1&S21REF=10&S21FMT=JUU_all&C21COM=S&S21CNR=20&S21P01=0&S21P02=0&S21P03=IJ=&S21COLORTERMS=1&S21STR=%D0%9669957http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I21DBN=UJRN&P21DBN=UJRN&S21STN=1&S21REF=10&S21FMT=JUU_all&C21COM=S&S21CNR=20&S21P01=0&S21P02=0&S21P03=IJ=&S21COLORTERMS=1&S21STR=%D0%9669957 -
61
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, (/): K2HPO4 1; MgSO47H2O 1; NaCl 1; (NH4)2SO4 2; CaCO3 2; 10; 1 . (/): FeSO47H2O 1; MnCl24H2O 1; ZnSO47H2O 1. 28 , 210 /, 72 .
(10 /). 4%. (/): 5; 10; 15; 10, 50, 60; 10, 20, 30; 10; 20; 30; 3,5; 6,5; 10. (NH4)2SO4 2 /. 21,21 %. (/): (NH4)2SO4 1; 2; 4; 1; NaNO3 2,5; NH4l 1,6; (NH4)2O3 1,44. K2HPO4, MgSO4, NaCl, CaCO3 1 , 1 , 1, 2 . (/): Na2CO3, - 2, Na2HPO4 1, Na2SO3 1, CaCl2 1, MgCO3 2, K2CO3 2, KHSO4 1, FeCl2 1.
, Streptomyces sp.12, , . , 3,5 / , 0,075 /. , , (NH4)2SO4 2 /. 0,145 /.
, Streptomyces sp.12 (/): K2HPO4 1; MgSO47H2O 1; NaCl 1; (NH4)2SO4 2; CaCO3 2; 3,5.
1. . . / . . ,
. . // . : . , 2014. 321 . 2. Ravi M. Studies on the potent protease producing bacteria from soil samples / M. Ravi, K. Rayudu,
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-
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2. Poulain M. The effect of obesity on chronic respiratory diseases: pathophysiology and therapeutic strategies / M. Poulain, M. Doucet, G. Major . // CMAJ174(9), 2006. 12931299 .
3. Vuorelaa M, Leinonenb M, Saikkuc P, Tammelaa P, Rauhad JP, Wennberge T, Vuorela H. Natural products in the process of finding new drug candidates // Curr Med Chemistry, 2000. 1375-89.
4. Nandini V., S. Devaraj N, Devaraj H. A fibre cocktail of fenugreek, guar gumand wheat bran reduces oxidative modificationof LDL induced by an atherogenic diet in rats // Mole Cell Bio, 2007. 145153.
5. Vyas Amit S, Patel Nailesh G, Panchal Aashish H, Patel Rameshwar K, Patel Madhabhai M. Anti-Arthritic And Vascular Protective Effects Of Fenugreek, Boswellia Serrata And Acacia Catechu Alone And In Combinations // Pharma Sci Moni, 2010. Vol-1, Issue-2.
6. Gupta A, Gupta R, Lal B. Effect of Trigonella foenum-graecum (fenugreek) seeds on glycaemic control and insulin resistance in type 2 diabetes mellitus: a double blind placebo controlled study //J Assoc Phys Ind. 2001. 1057-61.
7. Xue W, Li X, Zhang J, Liu Y, Wang Zh, and Zhang RJ. Effect of Trigonella foenum-graecum (fenugreek) extract on blood glucose, blood lipid and hemorheological properties in streptozotocin-induced diabetic rats // Asi Pak J Clin Nutr, 2007. 422-426.
8. Birjees Bukhari S, Bhanger M. L, Memon S. H. Antioxidative activity of extracts from fenugreek seeds (Trigonella foenumgraecum) // Pak J Anna Enviro Chem, 2008. 78-83.
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574/577:612.39
EFFECT OF POWDER FROM TRIGONELLA FOENUM-GRAECUM SEED ON BODY WEIGHT OF RATS UNDER CONDITIONS OF HIGH CALORIE DIET
I. Goloborodko, V. Konopelnyuk, L. Ostapchenko Educational and Scientific Centre "Institute of Biology" of Taras Shevchenko National
University of Kyiv, 64/13, Volodymyrska Street, City of Kyiv, Ukraine, 01601 Introduction. Overweight and obesity, as well as their related diseases, are considered a problem
of developed countries and developing countries [1]. aloric-dense diets, high fat, increased urbanization and psychosocial stress, sedentary lifestyles are the most common contributing factors [2]. Its prevalence is increasing in adults and children, and has been described by the WHO as a global epidemic with an estimated 500 million obese adults and 1.5 billion overweight or obese individuals worldwide. Obesity is associated with an overall increase in mortality and a decrease in lifespan of up to 20 years [3].
The most important reasons associated with obesity includes changes in dietary pattern, lack of physical activity, sedentary lifestyle, lack of sleep, endocrine disorders, irregular metabolism, medications that make patients put on weight, psychological and infectious disorders. Diet and behavioral modification programs for weight loss and the prevention of weight regain aim to reduce energy intake and to increase energy expenditure [4].
Today more and more to treat various chronic diseases commonly used herbal supplements because they have less toxic effects than chemically synthesized drugs and are cost effective. Recent preliminary reports suggested that herbs might be effective in reducing appetite and promoting significant weight loss are encouraging.
Thus medicinal plants and the chemicals derived from plants have gained the interest of public and scientists for their role in maintaining health and preventing disease. The medicinal plant with potential efficacy to prevent obesity is briefly discussed [5]. As one promising candidate, in this study, we investigated antiobesity effects of Fenugreek (Fg) in Hight Caloric Diet (HCD) -induced obese rat.
Objective. To investigate the feasibility of using and effect of powder from Trigonella Foenum-Graecum seed on body weight of rats under conditions of high consumption diet.
Materials and methods. Studies conducted on 40 Wistar rats and divided to four groups of 10 animals each. Rats of group 1 (Control, C) were given water ad libitum and were fed by a standard chow during 14 weeks of the experimental period. The 2 (HCD) group was fed by a high-caloric diet. Rats of group 3 (C+Fg) were fed by a standard chow which containing: fenugreek (2%). The (HCD+Fg) group was fed by, a high-caloric diet, which contained fenugreek (2%) during 14 weeks of the experimental period. Food consumption was measured daily at the same time (09:00 to 10:00 h) and body weights were determined once a week.
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70
Seeds of Trigonella foenum graecum L. varieties Ovari 4 were provided by Professor of the University of West Hungary Sndor Makai (Institut of Crop Sciences, Department of Medicinal and Aromatic Plants).
Statistical analysis performed by using Statistica 7.0 software. Values are statistically significant at P
-
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