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SIMULTANEOUSLY DETECT MULTIPLE TARGETS IN SINGLE ASSAYS

MULTIPLEXING

• Allowing researchers to gain more insight into precious and often low-volume samples

• Simultaneously interrogate multiple analytes in a single assay

• May or may not require specialized equipment

• Can increase diagnostic yield

MULTIPLEXING

miRNA

IncRNA

TargetedRNA

• QIAGEN miScript PCR System

• QIAGEN RT² Profiler PCR Arrays

• QIAGEN QIAseq Targeted RNA Panels

• Illumina TruSeq Targeted RNA Expression Kits

• Illumina TruSeq RNA Access

• Illumina TruSight RNA Pan-Cancer Panel

• QIAGEN RT² lncRNA PCR System

1.

DNA Methylation

Targeted DNA

Protein

DNA CopyNumberVariation

• QIAGEN RT² Profiler PCR Arrays

• QIAGEN QIAseq Targeted RNA Panels

• Illumina TruSeq Targeted RNA Expression Kits

• Illumina TruSeq RNA Access

• Illumina TruSight RNA Pan-Cancer Panel

• Illumina Infinium Methylation EPIC Kit

• QIAGEN EpiTect Methyl II PCR System

• Illumina TruSight Panels

• Illumina TruSeq Custom Amplicon

• QIAGEN GeneRead DNAseq Targeted Panels

• QIAGEN GeneRead DNAseq Custom Panels

• QIAGEN qBioMarker Somatic Mutation PCR System

• GenDx NGS-Go HLA Typing

• Multiplicom MASTR panels

• QIAGEN qBiomarker Copy Number System

• RnD Systems Luminex Assays and High Performance Assays

• RnD Systems Proteome Profiler Antibody Arrays

• QIAGEN ELISArrays

2.

miRNAQIAGEN miScript PCR System

• The miScript PCR System allows sensitive and specific detection and quantification of miRNA using SYBR Green real-time PCR

• miScript PCR arrays are provided in ready-to-use 96-well plate, 384-well plate, and 100-well rotor disc formats compatible with all popular real-time thermal cyclers

• Each assay in a miScript miRNA PCR Array has been bench verified to ensure sensitive and specific detection of mature miRNA by real-time PCR

• All miScript Arrays are partially or fully customizable for content

• Array package includes relevant controls and data analysis

• Arrays available in miRNome, high-content and focused disease or pathway specific formats

QIAGEN RT² lncRNA PCR System• For lncRNA expression analysis related to a disease state or

biological pathway

• Enables expression analysis of multiple related lncRNA’s at one time

• Use for screening, or verification of RNA-seq and microarray studies

• All qPCR assays laboratory-verified for qPCR performance

• Available for 384-well and 96-well plate, and 100-well rotor disc formats

IncRNA

3.

QIAseq Targeted RNA Panels• Traditional RNA sequencing methods suffer from PCR

duplication and amplification bias, resulting in inaccurate gene expression analysis

• By introducing molecular barcodes before any amplification takes place, QIAseq RNA Panels are able to eliminate this issue to deliver accurate and digital quantification of genes

• Data analysis modules have been developed that are comprehensive, yet easy to use. Using these modules requires no bioinformatics expertise

• QIAseq RNA Panel overview:

• QIAseq Targeted Panels - wet-bench verified panels

• QIAseq Targeted RNA Virtual Panels - build on QIAGEN’s know-how to deliver more than 170 panels with biologically and disease relevant gene content

• QIAseq Targeted RNA Custom Panels

QIAGEN RT² Profiler PCR Arrays• PCR Arrays are the most reliable tools for analyzing the

expression of a focused panel of genes

• Each 96-well plate, 384-well plate, or 100-well disc PCR Array includes bench-verified SYBR Green primer assays for a thoroughly researched panel of relevant pathway- or disease-focused genes

• PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests

• Arrays include well-designed controls to assess genomic DNA contamination, RNA quality, and general PCR performance

• The simplicity of the PCR Arrays makes them accessible for routine use in every research laboratory

Targeted RNA

Tired of pipetting? You're going to love the QIAgility!

For rapid, high-precision automated PCR setup

4.

Illumina TruSeq Targeted RNA Expression Kits• TruSeq Targeted RNA Expression Kits offer highly customizable

mid- to high-plex gene expression profiling and validation for desktop sequencers

• Targeted RNA assays deliver a fully integrated solution, including convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50ng of RNA, and automated, on-instrument data analysis

• User-defined panels of 12-1,000 assays can be created to target genes, isoforms, splice junctions, cSNPs, and fusion genes with the ability to multiplex up to 384 samples

• Generate data equivalent of up to 25,000 individual RT-PCR assays in a single run

• Fixed and custom panels available

Illumina TruSeq RNA Access• Using proven TruSeq Stranded RNA library prep chemistry

combined with efficient sequence-specific capture, the TruSeq RNA Access Kit generates RNA-Seq libraries from degraded samples (incl. FFPE) that focus on the RNA coding regions

• Isolating these high-value content regions maximizes discovery power, while requiring only a fraction of the read depth of total RNA sequencing

• The results are lower input requirements, higher sample throughput, and more cost-effective transcriptome analysis

Illumina TruSight RNA Pan-Cancer Panel• The TruSight RNA Pan-Cancer Panel provides a comprehensive

analysis of the cancer transcriptome

• Targeting 1,385 cancer-related transcripts and genes known to be involved in gene fusions, the TruSight RNA Pan-Cancer Panel enables analysis of cancer samples including FFPE tissues and other limited samples

• The Illumina TruSight RNA Pan-Cancer Panel offers:

• Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners

• Compatible with a range of RNA input quality, from high to low quality including FFPE samples

• A comprehensive view of cancer pathways

• Economical RNA sequencing (RNA-Seq) on a desktop sequencer

5.

QIAGEN EpiTect Methyl II PCR Arrays• EpiTect Methyl II PCR Arrays allow the simultaneous DNA

methylation profiling of a panel of 22 or 94 gene promoters using restriction enzyme based MethylScreen technology

• Genes are carefully selected based on their reported methylation status in a variety of experimental settings

• These arrays allow correlation of CpG island methylation status with biological phenotypes or disease outcomes

• Both EpiTect Methyl II Signature PCR Arrays (22 genes) and EpiTect Methyl II Complete PCR Arrays (94 genes) are available for human, mouse or rat studies in 96- or 384-well formats

Illumina Infinium MethylationEPIC Kit• The Infinium MethylationEPIC kit provides unparalleled

coverage of CpG islands, genes, and enhancers. Infinium HD array technology enables content selection independent of bias-associated limitations often associated with methylated DNA capture methods

• The result is a pan-enhancer and coding region view of the methylome that can be used for epigenome-wide association studies on a variety of human tissues. It includes the following content categories requested by methylation experts:

• CpG sites outside of CpG islands

• Non-CpG methylated sites identified in human stem cell (CHH sites)

• Differentially methylated sites identified in tumour versus normal tissues

• FANTOM5 enhancers

• ENCODE open chromatin and enhancers

• DNase hypersensitive sites

• miRNA promoter regions

• >90% of content contained on the Illumina HumanMethylation450K BeadChip

DNA Methylation

6.

Illumina BaseSpace Sequence Hub

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Push-button informatics - Let's work togetherIntegrate, analyze, and discover with BaseSpace Sequence Hub

Introducing the Illumina MiniSeq SystemA simple, affordable sequencing solution.

Powering molecular research and oncology applications.

7.

BaseSpace Sequence Hub is the Illumina cloud-based genomics computing environment for next-generation sequencing (NGS) data management and analysis. Sequencing labs can store and share sequencing data, and researchers can simplify and accelerate NGS data analysis with push-button tools. Labs can also set-up and monitor their sequencing runs in real time on any Illumina instrument. BaseSpace Sequence Hub can be accessed via an intuitive web-based interface or Linux-based command line tool.

• A simple, intuitive interface for managing genomic data

• Sequencing instrument integration for accessibility from anywhere, without requiring software downloads and installations

• Unlimited data storage space and processing power, on demand

• A secure and fault-tolerant platform for all data, both raw and processed

• An easy mechanism for sharing data with others

• A broad library of applications (apps) to simplify genomic data analysis

BaseSpace Sequence Hub provides:

TruSeq Custom Amplicon • TruSeq Custom Amplicon is a fully customizable,

amplicon-based assay for targeted resequencing

• Up to 1,536 amplicons can be sequenced in a single reaction using a simple workflow (allowing coverage of up to 650kb of cumulative sequence)

• Requiring as little as 50ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types

• Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling researchers to fully benefit from the desktop sequencer output capabilities

• TruSeq Custom Amplicon Low Input Library Prep Kit delivers sensitive and specific results from both low-input and formalin-fixed, paraffin-embedded (FFPE) DNA samples

Illumina TruSight Panels• For cost-effective, streamlined, targeted next-generation

sequencing (NGS) of specific genetic diseases or conditions, Illumina offers TruSight sequencing panels

• Designed with recognized healthcare experts at leading institutions, TruSight panels comprise of oligo probes targeting genes and regions thought to be relevant for particular diseases or conditions

Targeted DNA

• TruSight One - Targeting >4,800 genes; enabling labs to expand and streamline their assay portfolio and sequencing portfolio

• TruSight HLA - Accurate, unambiguous, phase-resolved HLA typing in a single assay

• TruSight Myeloid - Uses expert-defined content to identify somatic mutations in myeloid malignancies

• TruSight Cancer - Targeting genes previously linked to a predisposition towards cancer

• TruSight Tumor 15 - Focused panel assesses common somatic variants in solid tumours

• TruSight Cardiomyopathy - Focusing on identifying inherited cardiac conditions

• TruSight Inherited Disease - Focusing on severe, recessive paediatric onset diseases

8.

QIAGEN GeneRead DNAseq Targeted Panels• For targeted enrichment of biologically and clinically relevant

panels prior to NGS

• Outstanding sequencing performance (>95% exonic regions covered, >95% reads align to target regions, >90% of target bases covered at >20% mean sequencing depth)

• As little as 10ng DNA needed

• Compatible with many samples types including FFPE samples

• Can be used on Illumina and Ion Torrent sequencing platforms

• Clinically and biologically focused content

QIAGEN GeneRead DNAseq Custom Panel v2

• For targeted enrichment of a customized set of genes or genomic regions specific for your NGS needs

• Outstanding sequencing performance

• As little as 10ng DNA needed

• Compatible with many samples types, including FFPE samples

• Can be used on Illumina and Ion Torrent sequencing platforms

• Customizable for any region in the genome

• Each panel consists of multiplex PCR primer sets, sufficient for 480 samples, to amplify genomic regions of interest tailored to your specific NGS needs

• QIAGEN’s primer design and targeted enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples

9.

QIAGEN qBioMarker Somatic Mutation PCR System• Detection of mutations in cell lines or research samples is

critical for toxicological, drug development, and cancer studies

• qBiomarker Somatic Mutation PCR Arrays are panels of mutation-specific real-time PCR assays for simultaneously profiling mutations within a disease or pathway focused set of genes

GenDx NGS-Go HLA Typing• GenDx offers complete workflow, optimized and ready to go for

NGS-based HLA typing

• Support for Illumina, PacBio and Ion Torrent NGS platforms

• Benefits of GenDx NGS-Go HLA Typing:

• Pooling of HLA amplicons - save reagents and time

• Multiplexing up to 384 libraries per run - high throughput sequencing

• Paired-end sequencing - optimal phasing for high-resolution typing

• NGSengine software - one button analysis

Multiplicom MASTR Panels• MASTR assays offer an innovative combination of premixed PCR

primers in a ready-to-use kit, enabling enhanced target amplification for DNA-based diagnostics

• MASTR assays serve as front-end amplification for sequence analysis for all massively parallel sequencing (MPS) systems (Illumina, Ion Torrent and 454)

• The technology is based on “target amplification” rather than classic target enrichment

• MASTR kits offered for:

• Cancer genetics

• Molecular tumour pathology

• Genetic disorders

• Cardio disorders

• Prenatal chromosomal abnormality testing (NIPT)

10.

IDT xGen Target Capture for NGS• xGen target capture products from IDT enable greater

sensitivity and higher sample throughput for targeted next generation sequencing (NGS)

• NGS researchers can now benefit from the IDT high throughput, quality controlled manufacturing process to order gene panels that have the greatest level of uniformity. These panels can be easily optimized with the addition of more probes

• xGen Lockdown Probes are individually synthesized oligonucleotides that offer an unprecedented level of flexibility and scalability

• xGen Lockdown Panels are functionally validated, stocked gene panels for targeted next generation sequencing that are optimized for performance

ADVANCED ANALYTICAL PLATFORM FOR CLINICAL GENOMICS

Global Leader in Data Driven MedicineWorld’s Largest Clinical Genomics Community

11.

Sophia DDM® is the platform of choice for clinicians to perform routine diagnostic testing.

Core technologies: PEPPER™, MUSKAT™ and MOKA™ process and analyse raw genomic data to help hospitals better and faster diagnose patients across a broad range of diseases including oncology, metabolism, paediatrics, cardiology and hereditary cancers.

www.sophiagenetics.com

QIAGEN qBiomarker Copy Number PCR• The qBiomarker Copy Number PCR arrays and assays are

designed for highly sensitive detection and profiling of copy number alterations (CNA’s) and copy number variations (CNV’s)

• Copy number variations and copy number alterations are genomic aberrations that occur either in the germline or are acquired somatically, respectively

• Gene amplifications and deletions are major sources of genetic diversity that can cause Mendelian diseases, cancer and are associated with complex diseases and traits

DNA Copy Number Variations

12.

www.qiagen.com/geneglobe

GeneGlobeYour new research companion

Simple and Accurate Tools for Pathway and Disease Research Profiling

RnD Systems Proteome Profiler Antibody Arrays

• Proteome Profiler Antibody Arrays allow for the measurement of up to 119 proteins in a single sample

• These arrays require no specialized equipment and eliminate the need for multiple Western blot experiments

• Antibody Array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies

• The arrays utilize chemiluminescence for detection and membranes can be assessed for protein levels in the same manner as traditional Western blots

• Select arrays are also suitable for use with the LI-CORdetection system

RnD Systems Luminex Assays and High Performance Assays

• Simultaneously detect and quantify multiple target analytes in qualified complex sample types

• Luminex bead-based multiplex assays are designed to provide accurate, reproducible results for every target analyte

• Assays require small sample volumes, are cost-effective, and allow researchers to collect more data in less time than other assays

• Luminex Assays: Allow up to 100 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples

• Luminex High Performance Assays:The most accurate and precise bead-based multiplex assays for simultaneously measuring up to 22 analytes in qualified complex matrices

Protein

13.

QIAGEN ELISArrays• QIAGEN ELISArray Kits provide the simplest way to monitor

multiple cytokines and chemokines simultaneously

• Multi-Analyte ELISArray Kits: Profile twelve cytokines in six cell supernatant (media), serum, or plasma samples using one simple protocol. The ELISArray Kits provide researchers with a quick assessment of the level of multiple proteins in multiple samples

• Mix-n-Match ELISArray Kits: A custom panel of cytokines or chemokines is arranged in a 96-well microplate to your specifications. Researchers can select 12 individual cytokines or select fewer cytokines and increase the number of samples per plate

Model, analyse and understand the complex biological and chemical systems at the core of life science research

with QIAGEN Ingenuity IPA

Market Leading Pathway AnalysisUnlock the insights buried in experimental data by quickly identifying relationships, mechanisms, functions, and pathways of relevance.

Predictive Causal AnalyticsPowerful causal analytics at your fingertips help you to build a more complete regulatory picture and a better understanding of the biology underlying a given gene expression study.

NGS/RNA-Seq Data AnalysisGet a better understanding of the isoform-specific biology resulting from RNA-Seq experiments.

14.

INGENUITY®

Whitehead Scientific (Pty) LtdSouth Africa - Cape Town(tel) +27 (0) 21 944 6460 - (fax) +27 (0) 21 949 5478PO Box 194, Brackenfell, 7561, Cape Town, South Africawhitesci@whitesci.co.za - www.whitesci.co.za Multiplexing Brochure 07/2016Whitehead Scientific (Pty) LtdSouth Africa - Cape Town(tel) +27 (0) 21 944 6460 - (fax) +27 (0) 21 949 5478PO Box 194, Brackenfell, 7561, Cape Town, South Africawhitesci@whitesci.co.za - www.whitesci.co.za Multiplexing Brochure 08/2016

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