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Page 1: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

bioengineering

Biotransporters.Boost your PK/PD studies

to new levels. Introducing

the ADME Knockout Rat

collection from SAGE™Labs.

For more information or to order

a knockout rat model, visit

wherebiobegins.com/bioengineering

Page 2: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Sig

Four ADMEresearch partnersyou need to know.Meet our ADME Knockout Rats. With greater

predictability than chemical inhibitor assays,

they’re better by design.

SAGE is a trademark of Sigma-Aldrich Co. and its a�liate Sigma-Aldrich Biotechnology, LP.

For more information or to order a knockout rat model, visit

wherebiobegins.com/bioengineering

Screen xenobiotic compounds in research

applications such as:

• Drug transport across the blood/brain barrier,

liver, intestine and placenta

• Drug resistance studies

• Drug metabolism

• Chemotherapeutic resistance

Observe in�ammatory stimulus response and

chemotherapeutic agents in research applications

such as:

• Drug transport across the liver

• Drug resistance studies

• Excretion pathways in cancer

Advance the study of anti-cancer compounds

as they relate to:

• Drug transport across the liver, small intestine

and kidney

• Drug resistance studies

• Drug metabolism

• Excretion pathways in cancer

• Dubin-Johnson syndrome

E�ectively screen for P-gp drug interactions

as they relate to:

• Drug transport across the blood/brain barrier

and intestine

• Neurotoxicology

• Dosing/Bioavailablity studies

• Drug resistance studies

Knockout of Bcrp Gene Knockout of Mrp2 Gene

Knockout of Mrp1 Gene Knockout of Mdr1a Gene

0422Product_FOCVR3 385 4/23/11, 10:34:56 AM

Page 3: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Moving science forward

©2011Therm

oFisherScientificInc.Allrightsreserved.

NowSoldas

Phusion ®

Therm

oScien

tific

Best-In-Class High Fidelity DNA Polymerases.

Everything for PCR

Thermo Scientific PCR portfolio has

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plastic consumables.

Thermo Scientific Phusion High-Fidelity DNA Polymerases are the

established standard for high-fidelity PCR. Phusion reagents were

introduced in 2003 by Finnzymes, which is now part of Thermo

Fisher Scientific. They are the first choice for several demanding

PCR applications, including the creation of the first functional

synthetic genome.

• Accurate – the highest fidelity of all thermostable DNA

polymerases

• Robust – abundant yields with minimal reaction optimization

• Fast – high processivity allows short protocols

• Specific – hot start version minimizes non-specific

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All Finnzymes products including Phusion reagents are now sold

under the Thermo Scientific brand.

Try Phusion today: www.thermoscientific.com/phusionproducts

Page 4: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

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with Intelligent Packing.

Want to know more? Register today to receive a copy of the ÄKTA avant 150 Data File.www.gelifesciences.com/pr-avant

Page 5: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

US & Canada | 1-877-772-2622Outside NA | 650-583-6688

www.epitomics.comFor updates and special o�ers

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MONO

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Rabbit Monoclonal Antibodies (RabMAbs®)

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Findmore @www.epitomics.com/advantage

What is the Rabbit Monoclonal Advantage

CDX2 RabMAb

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and Vendor A’s CDX2 Mouse Monoclonal under optimal conditions

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Page 6: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder
Page 7: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

OneTaq™ DNA Polymerase

THE ONE YOU’VEBEEN WAITING FOR

• Robust yield with minimal optimization

• Ideal for routine, AT- or GC-rich templates

• Hot start and master mix versions available

The ONE polymerase for your endpoint PCR needs

Amplification of a selection of sequences with varying AT and GC content

from human and C. elegans genomic DNA, using OneTaq DNA Polymerase.

10.08.06.05.04.0

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kb M 29 37 47 55 65 66 73 79 %GC

AT-rich Standard GC-rich High GC

Standard Reaction Buffer GC Reaction Buffer

Plus High GC Enhancer

www.neb.com/OneTaq

Request a sample at

Page 8: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Your local distributor www.eppendorf.com/worldwide · Application Support: [email protected]

Eppendorf AG · Germany · Tel: +49 40 538 01-0 · Eppendorf North America, Inc. · USA · Tel: +1 800 645 3050

Eppendorf Asia Pacific Headquarters · Malaysia · Tel: +60 3 8023 2769

Laboratory consumables can affect bioassays!

Reaction tubes for storing and processing samples

should be selected carefully as they can influence the

quality and reproducibility of your entire workflow.

Original Eppendorf Tubes are produced without additives

that have been shown to influence bioassay results, offer

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Experience Eppendorf quality consumables and

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Go for Unaffected Assay Results!Choose the right tubes for your experiments

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Page 9: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Page 10: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Congratulations to Dr. Christopher Gregg on winning the 2010 Eppendorf

& Science Prize for his studies on genes that alter their expression in the

brains of offspring according to whether they were inherited from the

father versus the mother. His findings suggest new pathways that may

help to understand brain diseases such as autism, schizophrenia and

eating disorders.

The annual international US$ 25,000 Eppendorf & Science Prize for

Neurobiology honors young scientists for their outstanding contributions

to neurobiological research based on methods of molecular and cell bio-

logy. The winner and finalists are selected by a committee of independent

scientists, chaired by Science’s Senior Editor, Dr. Peter Stern.

To be eligible, you must be 35 years of age or younger. If you’re selected

as this year’s winner, you will receive US$ 25,000, have your work

published in Science and be invited to visit Eppendorf in Hamburg,

Germany. Past winners and finalists have come from as far a field as

China, Chile, India and New Zealand. Yes, it can happen to you!

Deadline for entries

June 15, 2011

2010 WinnerChristopher Gregg, Ph.D.Postdoctoral FellowHarvard University

It´s easy to apply! Learn more at

www.eppendorf.com/prize

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rstin

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Be the

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Get recognized!

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Page 11: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Dr. Carl Alving

on his inspiration

for inventing

the vaccine patch.

“A dreamtoldme todo it.”

MemberCentral is the new website that looks at science through the eyes of AAAS members. It

celebrates their achievements—like Dr. Alving’s vaccine patch—and their shared belief in the

transformative power of science. Use MemberCentral to connect with other members, learn

about work being done in other fields, and get fresh perspectives on issues ranging from

speciation to STEM education.

VisitMemberCentral today and get to know the

AAAS member community in a whole new way.

Blogs I Videos I Webinars I Discounts I Downloads | Community

MemberCentral.aaas.org

Dr. Carl Alving

on his inspiration

for inventing

the vaccine patch.

“A dreamtoldme todo it.”

Carl R. Alving, M.D.

Chief of the Department of Adjuvant & Antigen Research,

Division of Retrovirology

at the Walter Reed Army Institute of Research

AAASmember

Page 12: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Submission

deadline

August 1

imagination at work

* For the purpose of this prize, molecular biology isdefined as “that part of biology which attempts tointerpret biological events in terms of the physico-chemical properties of molecules in a cell”.

(McGraw-Hill Dictionary of Scientific andTechnical Terms, 4th Edition).

GE Healthcare Bio-Sciences AB,Björkgatan 30, 751 84 Uppsala, Sweden.© 2011 General Electric Company– All rights reserved.

28-9402-06AB

Imagine standing on the podium at the Grand Hotel in Stockholm, making your

acceptance speech for the GE & Science Prize for Young Life Scientists. Imagine

having your essay read by your peers around the world. Imagine discussing your

work in a seminar with other prize winners and Nobel Laureates. Imagine what you

could do with the $25,000 prize money. Now stop imagining. If you were awarded your

Ph.D. in molecular biology in 2010, then submit your 1000-word essay by August 1,

and you can make it reality.

Want to build a better reality? Go to www.gescienceprize.org

The GE & Science Prize for Young Life Scientists.Because brilliant ideas build better realities.

Yournamehere.

Page 13: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Leonard Zon, M.D.HHMI/Children’s Hospital

and Harvard Medical School

Boston, MA

Hakim Djaballah, Ph.D.Memorial Sloan-Kettering

Cancer Center

New York, NY

Gregory Verdine, Ph.D.Harvard University

Cambridge, MA

PARTICIPATING EXPERTS

During normal growth and differentiation,maintaining the appropriate homeostatic balance ina cell is paramount. This fundamental level of controlis achieved through the interaction of small moleculechemical modulators with those proteins involved incellular signaling. Researchers can take advantageof these regulatory interactions to study signalingpathways, for instance by designing chemicals thatreverse the hyper-proliferation phenotype seen inmany cancers. These small molecules can be usedboth as tools to interrogate a particular pathway andas precursors for drug development. This webinarwill provide an overview of how chemical biologycan be used in various model systems to bothadvance our understanding of basic cell signalingmechanisms as well as aid in the discovery of novelsmall molecule therapeutics for cancer.

During this webinar, the panelists will:• Provide an overview of how chemical biology can

impact cancer research

• Describe their own research using smallmolecules to elucidate signaling pathways andcharacterize their component proteins

• Give you the opportunity to ask them questions,live and in real time.

May 3, 201112 noon ET, 9 am PT,4 pm GMT, 5 UK

WEBINAR

Probing Cancer PathwaysHow Chemical Biology Can Inform

Oncology Research

Brought to you by theAAAS/Science Business Office

Sign Up At:

REGISTER NOW!Webinar sponsored by

www.sciencemag.org/webinar

Page 14: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

489www.sciencemag.org/products SCIENCE VOL 332 22 APRIL 2011

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are

featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or

materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

LIFE SCIENCE TECHNOLOGIES

NEW PRODUCTS

AAAS/Science Business Office Feature LIFE SCIENCE TECHNOLOGIES

NEW PRODUCTS

AAAS/Science Business Office Feature

CELL COUNTER Designed for laboratories that routinely work with cells tagged with a large

variety of fl uorescent labels, the new Cellometer Vision allows users to easily

change out either of the two fl uorescence optics modules in the instrument

and replace it with another one. This enables scientists to perform experiments

using their choice of fl uorescent labels, with a broad spectrum of excitation and

emission wavelengths from ultraviolet to red. Using proprietary patent-pending

image processing and data analysis algorithms, Cellometer Vision combines

brightfi eld microscopy and one or two fl uorescence colors to count and analyze

cells that are loaded into a disposable chamber. A wide variety of fl uorescent

dyes may be used to stain the cells, providing clear declustering of the cells to

generate reliable viability results.

Nexcelom Bioscience

For info: 978-327-5340 www.nexcelom.com

LIVER MICROSOMES A comprehensive range of liver microsome products from over 12

species, including mouse, rabbit, monkey, and chicken is now avail-

able. Researchers can select from pure microsomes or S9 fractions,

or animal liver cytosol. In order to facilitate requirements recom-

mended by REACH, bespoke microsome products from other spe-

cies or strains can also be supplied. Microsomes are a valuable tool

for investigating drug metabolism and examining in vitro drug-drug

interactions. They are produced by mechanically homogenizing cells

to fragment the endoplasmic reticulum, the fractions of which reform

into microsomes and are collected via differential centrifugation. Dur-

ing this process, the numerous different phase I cytochrome P450

enzymes are concentrated within the microsomes. The S9 fraction

contains microsomes as well as cytosolic phase II metabolism-relat-

ed enzymes.

Sera Lab

For info: +44-(0)-1444-250010 www.seralab.co.uk

CLOUD COMPUTING FieldAlign V3.0 is the latest in Cresset’s new generation of cloud-en-

abled applications, which support parallel, distributed computing by

default. It can be deployed both as a traditional desktop application

and as a command-line application distributed onto large computing

servers. FieldAlign is a powerful molecular design and 3D structure-

activity relationship (SAR) tool that generates biologically relevant

molecular comparisons, which can be used to fi nd the root causes of

activity or inactivity. FieldAlign helps chemists to gain detailed under-

standing of the SAR of their lead molecules and to use this to design

the best next synthesis. FieldAlign V3.0 introduces a range of user

features that improve productivity and customizability. Its command

line interface supports scripting and workfl ow systems, and is now

available on Windows, Linux, and Mac platforms. It incorporates a

new molecule table enabling fi ltering and sorting of lead molecules,

using imported data or standard physical properties such as wcLogP,

TPSA, and Rule of Five violations.

Cresset Group

For info: +44-(0)-1707-356120 www.cresset-group.com

RNA, DNA, AND PROTEIN ISOLATIONTri-Xtract is a convenient, ready-to-use reagent designed for the iso-

lation of total RNA, DNA, and protein. Tri-Xtract is suitable for the

isolation of total RNA from small quantities of tissues and cells from

human, animal, plant, yeast, bacterial, and viral origin. Tri-Xtract is

a monophasic solution of phenol and guanidine thiocyanate that

maintains the integrity of the RNA during cell disruption and homog-

enization. The isolation procedure takes less than an hour and the

isolated RNA is suitable for Northern blots, dot blot hybridization, in

vitro translation, RNase protection assays, and poly (A+) selection.

G-Biosciences

For info: 314-991-6034 www.gbiosciences.com

FULL-LENGTH HUMAN PROTEINSThe new range of over 4,000 full-length, affi nity-purifi ed human

proteins, which are expressed in HEK293 cells and derived using

an extensive collection of human cDNA clones, is currently the

broadest offering of human proteins produced in human cells. It has

been prepared to ensure optimal preservation of protein structure,

posttranslational modifi cations, and function. All full-length proteins

come affi nity purifi ed and are guaranteed to be greater than 80%

pure, making them ideal for use as native antigens for optimized

antibody production, positive controls in antibody assays, enzyme-

linked immunosorbent assay standards, protein-protein interaction

experiments, in vitro biochemical assays, cell-based functional as-

says, and SRM/MRM standards. All the human proteins in the new

range are available in small trial-size quantities, which can be quickly

developed into larger (milligram) quantities.

AMS Biotechnology

For info: +44-(0)-1235-828200 www.amsbio.com

FREEZE-DRYER AND LYOPHILIZATION SYSTEMThe latest freeze-drying and lyophilization innovation for improving

process control and minimizing issues of scale-up, the LyoStar 3, is

confi gured with the most innovative controls and instrumentation,

including Smart Freeze-Dryer Technology and Praxair’s ControLyo On

Demand Nucleation Technology. The LyoStar 3 is the ultimate lab-

scale system for formulations studies, process optimization, and

scale-up work. The system is a freeze-dryer that provides unmatched

process accuracy, reliability, and unique ease-of-use features. Until

now, the freezing step of lyophilization was uncontrolled due to the

random nature of the nucleation or crystal-forming process. Using

ControLyo technology on the LyoStar 3 now makes nucleation con-

trol a reality by regulating the nucleation temperature to within 1°C

of its freezing point. The results are improved product uniformity,

quality, and yield.

SP Scientifi c

For info: 845-255-5000 www.spscientifi c.com

Page 15: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Congratulations to the

AAAS Student Poster Competition Winners

The 2011 Student Poster Competition took place at the AAAS Annual Meeting in Washington, DC, 17-21 February. The students’

work in a variety of fields displayed originality and understanding that set them apart from their peers. First-place winners receive

cash prizes thanks to the generous support of Subaru of America, Inc.

BRAIN AND BEHAVIORWinner:Maria R. Torres, University of

California, Irvine

Investigating the Role of Down Syndrome

Astrocytes in Dendritic SpineMorphology

Honorable Mention: Nicholas D. Olivas,University of California, Irvine

Cell-Type Specific Excitatory Connectivity

of Hippocampal CA1 Interneurons Revealed

by Laser Scanning Photostimulation

CELLULAR AND MOLECULAR BIOLOGYWinner (tie): Kimberly Rebecca Kukurba,

Arizona State University

Genome-Wide Discovery of Human T

ranslation Enhancing Elements

Winner (tie): Aida Mohammadreza, Ari-

zona State University

Measuring Oxygen Consumption Rates

of Esophageal Cancer Cells at the Single

Cell Level

Honorable Mention: Nathan Gamarra,

University of California, Irvine

Antibiotic Resistance in Aquatic Stentro-

phomonas maltophilia

DEVELOPMENTAL BIOLOGY,PHYSIOLOGY, AND IMMUNOLOGYWinner: Dayan Li, Harvard University

The Role of PRDM8 in Sonic Hedgehog-

Dependent Neural Tube Development

Honorable Mention: Cristian Aguilar,

University of California, Irvine

Epigenetic Modifications During

Dedifferentiation in the Axolotl

Regenerating Limb

ENVIRONMENT AND ECOLOGYWinner: Nathaniel Mueller, University of

Minnesota

Are Changes in Agricultural Management

More Crucial to Food Security than

Climate Change?

Honorable Mention: Erica Warkus,

Arizona State University; Martine

Wagstaff and Scott Morello, University of

Massachusetts, Boston

Do Macroalgae Use Allelochemicals To

Outcompete Invertebrates for Space in

the Gulf of Maine?

MATH, TECHNOLOGY, AND ENGINEERINGWinner: Yakov Kronrod, Chang Hu,

Olivia Buzek, and Alex Quinn,

University of Maryland

Using Monolingual Crowds To Improve

Translation

Honorable Mention: Charles A. Forman,

Virginia Polytechnic Institute and

State University

Superconducting RF Accelerator Cavity:

Examination of the Interior Surface

MEDICINE AND PUBLIC HEALTHWinner: Allen Clayton, RachelM. Serafin,

Chau C. Quach, SethW. Brittle, Stephanie

Cabey, and Christine Gurdon,Marist College

Emerging Contaminants: Silver Nanopar-

ticles Bioaccumulation and Genotoxicity

in Aquatic Organisms

HonorableMention:Hansa Thompson and

Susan Holechek, Arizona State University

Independent Expression and Analysis

of the Two Domains of Vaccinia Virus’s

E3L Gene

PHYSICAL SCIENCESWinner:Matthew R. Edwards and Clayton

E. Myers, Princeton University

Temperature Effects on Electrical Proper-

ties of Superconducting Flux Conservers

Honorable Mention:Makrete Krikorian

and Jeffrey Meisner, Columbia University

Single-Molecule Conductance of

Disubstituted Stilbenes

SOCIAL SCIENCESWinner: Eric Michael Johnson, University

of British Columbia

Demographics, Inequality, and Entitle-

ments in the Russian Famine of 1891

Honorable Mention: Trent James Bowen,

Arizona State University

Educational Barriers to Preventative

Medicine in Accra, Ghana

Full abstracts can be viewed atwww.aaas.org/meetings.

The 2012 Poster Submission site opens on 12 July 2011. The Student Poster

Competition recognizes the individual research efforts of undergraduate-

and graduate-level students who are enrolled in a degree program. Posters

are judged at the meeting. Winners in each category receive a cash award

and framed certificate. Individuals holding doctoral degrees are not eligible

and are invited to submit for the General Poster session.

Sign up atwww.aaas.org/meetings to receive announcements about the 2012 AAAS Annual Meeting 16-20 February in Vancouver, BC.

Page 16: Biotransporters. · bioengineering Biotransporters. Boost your PK/PD studies to new levels.Introducing the ADME Knockout Rat collection from SAGE Labs. Formoreinformationortoorder

Life Technologies offers a breadth of products DNA | RNA | protein | cell culture | instruments

©2011 Life Technologies Corporation. All rights reserved. The trademarksmentioned herein are the property of Life Technologies Corporation or their respective owners, unless otherwise noted. CO18368 0311

go to www.invitrogen.com/benchpro2100download the free mobile app at http://gettag.mobi

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steps, this innovative instrument enables walk-away automation with only five minutes of hands-on

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all of the tedious workflow steps.