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"·,_ .. ,_ '-STUD_IES ON BIOCIDAL PROPERTIES OF SOME ORGANOTI.N CO-OR,DINA_TION COM.POUNDS . FUNGICIDAL AND· PHYTOTOXIC PROPERTIES THESIS SUBMITTED FOR. THE DEGREE OF DOCTOR ·oF PHILOSOPHY (SCIENCE) . OF THE - -. UNIVERSITY OF NORTH BENc;;AL By . ASIM KUMAR MALLICK, M. Sc. DEPARTMENT OF CHEMISTRY ( FUNGICIDAL LABORATORY) UNIVERSITY OF NORTH BENGAL 1982 I

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"·,_ ..

,_ .·

'-STUD_IES ON BIOCIDAL PROPERTIES OF SOME ORGANOTI.N CO-OR,DINA_TION COM.POUNDS .

FUNGICIDAL AND· PHYTOTOXIC PROPERTIES

THESIS

SUBMITTED FOR. THE DEGREE OF

DOCTOR ·oF PHILOSOPHY (SCIENCE) . OF THE

- -. UNIVERSITY OF NORTH BENc;;AL

By .

ASIM KUMAR MALLICK, M. Sc. DEPARTMENT OF CHEMISTRY

( FUNGICIDAL LABORATORY)

UNIVERSITY OF NORTH BENGAL

1982

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tt\ ·Q!i1~~. ~~ttf1o.io.Ug int'aQtoti t?!ti.tb the oe•·4~

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~n .t~~; ·~lli~ble: ;fo~ thl!} t'Ceatt.1fl:tl;t !C}t ~:)~ttl$•

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.t1~l11U -~tho und~evctapet ~oMtt1$a(40),. tlt~ ~at t¥rfi;J.i:!linc ~lJ' to :u~~~ t~- lit~ ·€qQli1ie oi' tb~ JJS8e1 t~• 1&

tho ~~31~ -t:dfit~_rol r;,lt the ~~t~:~ ei1MUG• ·~t~b.~l tin I

e!ltor~e, _n.n4 .an;;t:at~ tm$ bi8l:t~ aot:.t'f$· a~not DJf:i~li~~ '~ .~~~~ . , . .3 ntt'l· ,:i·~f:l! A~~A~~'IirtJ( 41) G'i ... n. ""'<I:: fl . <\ • .A 4) ~~ &lt'l>, ~ ~~ . • ~~p -1i,~fn0 ~~~,~~~· ·~() .

~ill tno -1.l.$ .d~v~.s sn.. :trtHt con~M\t~'tioo.. t t!l~ ~:tl:l thf' tlrl5Ua til e4· h()~, whsr~ll o •. ~ -rndl .. ta 41J hQur"fi~ ~bs­

bieh ~~Pxicit~ of Gr(t$rl+:'>1i1n c~~pot~i19 -~ ottw~ .fsms tJf ~

~uati<! l1£e 'J?~$$ntna -~ne t1!9Mtw,nt~eo. A ·oote n~J».4 .f~i' th~ ·_·1-ntv~ntuoti~ of or~t!~ tDxt~..nta· .ooe bota. ,)1--.-.·.I'. '1' _· .-.c.=. h.,_·. •. . 'i"i_.-. AI!·-·/· .. (42) 'f' ,.,,.,. ~ ~' 'h ....,.;t,i. . f; -lilw.v ~ ·tYP~<M · ~ ~r:)~o4;0~ ~- !;~·OO!.hi~y ·~ •u • tn·.~:t:U ~·Q Ql* J:1tl.V~

tt~~~ea ~~• ~~ ·mo:tluee!G18~~ ~o?::J t11~ ~o~.u,t t)f n~totur~

· .;,·d•ftvJ~ n"at¥{~~··"*".ab,tf... (43).: 'IJ;V-Y. __ - ,.-~ .. ' !~~-- t~~~ .. ~ -~

!1.. n~j~r. of o~~li«m~ ootb $1i~t3 ®tt ~,~;if~$ $n

~ow t\U tb~ t~J:t1 ~Jlli 6tll~:r f)ut!t$· of ellt,a o,n(l; ~"~!:O\ts

otber t:ibj6ete ~~.u~<t tn.. tteil -.-~t.e);".- 1;Mo tt1~4: of ~~tb

. te ~l.llled f{)W;iud lfmd .. can 0f)r.lotUJ14' .ttr1)nir ·the £uopt,io,tt

of ·ttJ.e a*a!,~o,..rt objoet$ whirJh •a 1{la4 to inO-~~s$4 <1®8t~n~

~ion ~)f. £flQi. O!Ad O't:M~ (\1;trt1!_0.t~l:.tf,.0rJ~ Zlot'lt ~l,iiti100 . . . . .

, . !ndu~~tw' l.t;t!$~ r;tilt~(n{J: o!: . d~~/l~$ t)/f/fjllrj/ lf~ dU.\'J to t~Ulin&•

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-~n :l~s tJ'iqtb$·llt.;;t;G · aleatt~ a!~i\ tritj;t~.titt~,

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t~~~-~•4l~ ~~-~ .. ~~,(l,.··~Jtvfl~ ~iter ~n,t~1 tott·£mt1to~\1~fl . t~~~ ·Gt~•- -~~voOO.tl$:\ ·~J,.-, ~:cibu.G1:tt!~Jo itu-):et{lo 1~ t1~. · ·in Jar~~~~ ~tit~n:~ a.g$.t., ~~l~~~nt ot' oll~t'lt\

· :~a~·antit~~: p~lnte ~- oo• ;c*new~· t.(f ~w.t~~~461.• ue-v.~),.. ·tot:mr:4~U$ --~ ~t.· :o-~e4t b\1!$-ne;. us~,' lro!l' o:r@!.t¥!i­

t.1,r~ ·-a~ttfi~I~l.t~g ~s~·(1rt0~

(Viti) ·~1.t.~llt\~.t\~#>l~t&

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:rt2~$J• r42 t~1llfitt:tit .it • m0t~tlt etl'Wl•· i~&~l1r»i.).V.t• .4 ... n-.1t,tl o~ - • •• • ~- r

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r~fUWl:J ~- # ;:~, Ol·• Drt ~ ot~· 11~· (ud L = · o or a ®..'lO:'

o~mo :tt~as ·tlei.Ve be~n £!)una to vo·,•e$S t~D-~~u.o:tbt•

th~'eutto liQ;t1.v1~ jQ -~~ tor;~au .i•3&6 lt4l&hoO:J'ti~

l~tat:111s i!t at~~. t)U;."':'t!otU.W?ll the liiuttql t1n tlfle.l1.4~

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-~oof~1 flti~t -~ · •: :fa'~o.r~ &: h\ltch. vote~l.t ol~la<;d tllo.t

t-vD\~l.t:m ohlCJrtd~0. e.a;;~, oo~¥~1 1n.-ote tltbo:V t~. ~r, . ~~~ .

t:lOtlla\: · v;, •; Dl~k.t &t ·.!(1' .. ". i .. •ttati 'tb~t tt~i!sfil~l.t:Ul d():Ut~)Q~$

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,t\<ettt;U$4. !~V.~$t~:tll~~& t:.<i- e OO~®tiJ:,. ~t~l,$JO:®d~ to

.:ttn~l' $ h:!gl• f!ot1V'it.s' .for the; trteubCJti tu.t.ed C!lnf>etmd&

C.wari· = tJ•l ~.1 ,PF/t'V,f.,], ~ ~o&J~t~- ·*let1~t1 :i'or c~:l­·:;;t1uA~•· Qt tml types :.~Ch w.a· Us~~tt2 /_.,. ·ttruo ·r; a61J'Lr/f~j.

. .

. ~ a 1~~ aotlv-t.ty £<)~ tt\,sft G£ ·tM type· aun.la• ~he SI1oil~~: ~8JUl't Wtt.$. qbta1mi -~ ~l~G $$ &l~GQ) &o~n !?t $1, (5$)·

e-YJt..u4f1tt the. m.tnbel" of tnooot.a nvcoit!te tG Uolule £1.10~~ .. - . .- - - "' b- bl:4l58t. ~"l~~aQhetl,t !;1GSltl:$.tos l\tlfl ft~llt· t).n\1 ObOot'yetl

.m~ t!l ·tW~~$f)Ontl ~1t;tb t-e.fi obea~ri bt Blil.~ et •t an thfj. ·Qti~ ot nte• .. · Ku\l.Q tc;a)· t~una ooa0 · t'tl~t>b~~lt·itn olt'~~ttbn~~~l~t•~ ae ooo·~ct ~ti;l.(lt1cidt$ ~;.~.u::,at ~~· ~aut~

' '

A~Wttb~} ~Vi-1. (Q,!11"fQ~S?JJU ~'~. "~~®& othflr

~t-ttct·rJ ·arm. t.t1tt~ tJC t1~~~oll~4 'ttf O~~t?'tt.f~ t)t:J~~i}Otmt1$~ OA$

of ·~t'lb :Ultfl'~"ttng n~:p$4t of orf~lll.t)t!\.u ootl;)O~"' 1n Ofl~troll•

~· th& itw~~tB .t~. toot,· tn @d,~::t1®. to a to~!i.Q oJ:f~~,

. tbtt• Qt1J~~~(le .. ~ ~:S:~~t ~a !l~t~f'ee'ewnt. cf.!~ot~~l.~ f~l.Oth~:r -e:11te~t e•~bed -~· ot'~ao1;in eoot)ottntltl· at lel1f

O.o.£>,.o~t~t1~lc~ ~a. l,tt~i1JJfti o~ d~~etmte~ lUJJ;.'J t:~it;b~~

ii~ ~:l~~H)\ftc{i~ ~$ ·tn~t~ Q,\)JJ.J;;t)! 1.'!) ut~n·l.~ ~1tb. irJ.S~t

·~l"tld~otion~ t~~~if4(Nl ~l!~rv~ tl'dle ~ft~t ~tli·. ~~~· ~lot$~., G!mil~ ~ifiet; b~ be~n O:bMe~~ .on l4~~~u. Ot)~•:

· ~¢b. (~\l$. ~5&) t"rAtlthe (J~nrtl~d f1ntW ·btJe:f:l.~.

(~~-~···· •'

(~)~Art$!~~

. ~;~ 'Yll,)$t ·or.>:tik~u ~oe f)f d(lftSQy. ot ~a tt) fl~l · · · .. Qt~:ett~. t~in:~~ · "~~~t~bi CQzJ9l;)ttn-gtJ. · at~w _f#t:r¢00. ~i~tl1ty.,

. not u,~l.!Julata~(SS) · e\~~$6 th~~ t,;;10t~:t .~ t~Rbutyti;:tn ,·.

-28-

compounds are bigbl,y. active 'B.gainst wood destroying .fungi,. . . , . ' .

I The uae Qf trietbyltin compounds .·must be discouraged

because of their disagrees.ble handling properties and higb.

taanma.lia.~ -toxici_ty.,· On the other b.a.nd t:ributyltiu compound~

o:fi'er iTa.st possibilities ... In recent years: there a·ra more ..

. than 60. wood preservatives based on T"B"TPQ., in the U~K.o

marke~' 56> .. ·.(XI) Miticidal activities

.• ·

·• . In the year 1968, . tricycloheXy'l tin .hydro::cide, the · .

. result of a joiri research effort by M & T Chemi:ca:Ls and

Dow Chemical Co., was :i,ntroduced under the name 'Pliotrant

for controlli~~ the phytophagous (p~ant-feeding) mites (57) . . .

on apples and pears ., It is now also used on citr~s 9

:·stone fruit, .hop,. teas vegetable e~c. :E"lictran is very

·selective and gives good control ot harmfuJ. _arachnids with

·little toxicity towards other insects such as honey .bees., ~ . .

It shows also the antifeedant activity against some .insects ·· ·

· -~a.t"";ae (58). There are now three other org~.o.otin miticides . .

of follo\ving types.. · · .·... .. . .. . . . . .

,·.

~(@. CH3 ) J. ·. Q . -f~cH2 -sn o

. . . . · CH3 . . 3. . . ~- , - • '·J. . • .

. · :. · · . VENOE X· ( SHELL )

PEROPAL ( BAVER, CHEMAGRO )

(a·. ·. .·_ft;;·oR Sn __:. S ...;. ·P .:-;....

· ···~.OR . '3

R -28627 ( STAUFFER )

. '

.ll~~iWJ '~'l~·- t11\V'h~o~bltg, 'tbG pets'&1~fiiet$ ~well.

tole~ttl1d b:t tm<)pil which u.o~Z.Jt ~e(4~a:~~ an aea~~c,l~,(! ' .

t~ pztt)t~ot; t~ ~a~t the ~if. D~llt•, $\p&t.te-a, GmtJet.

t.~to. MQ~,; tabb&g$. a~r.Q. oatt~n pl;tJ~.-t•• all soowta oooa tGltl~o• t:t> ~~e~o~1(5~>-.

Oon,t;..T\:)1 of. ~l G!ttee-s ill ~1¢~:Q.t~ ~

OV--4)tin Oi»mt}OUll~ia .i8 fil-.K)~b:ty th& Sll()f}ild ~(!Gilt St,'JJ;tl!~

Qo;tl<m of ~;r.-;:;i)t~.ll9•· 'Qhf! ftb?at ortl~:;;()~in cor..1:;,oW1-1 t~-...

pb.~):ti~. ~t~awto ((i,lao· ~11~ t~nt1ft. ~QG'tat~· (.tr D~!itla.~) w~ itt"ttt100~: Q~·tm®t1at~'iU.r O.Cfl et· f1Angl~i!!e tn ZW10D~ ·tn.

196..0• tw ~t-work~ tb$)bet ~ :a..~~tlY te.tw ;.Jblltpe ~uvmr

.1~n.~t!Qe~ fet1tl.fi ~tllJOleide Ct.~tled D~; ~- ~l\lr) t'l!th· e~~lv~

in&JfMlj;,t.~~t of tvi;vb.f!tqt1t1n iwaro~d«~• ~tb· »rt!~ten Ol'l4 ~a'•~t·"~ ·"-·_'"' · V'iiit. .,.._..,.~ · Mli t:Ji·q'Jt.kn_· ~ . ....m-a··':'" ""-_. ',p .J'!'.t;A..,_II!'j~io,. .~'A!!\1'<.-...~ it_ •. ( f$0} ·· h.'W. · -~~ il~.""" ~--«~ l&l',t• """'V~{A;I:J< ~-"~ ~W~~ ~J.II--.IJ-.'\,10~ •

-· '

~1-tin e.c~-t$' (lit"~~~-

{lj/'·;·'· J f I, ,

, I (: //

;.

~r1*.ph~tWl.ti.n acetate o~ 1\yd~:d.de ~- lt~ooi!llle$ed

iav the .cont~ol ()f! ·J21~Jll.til\G. ( 1o.t0 blight) ~ITA. oota.toea . .

(l.Jl.tf, 5!m!~ on ftl~r :ooeta. a't· the. ~te ot few 5ram :p~r . .

nQX'e.__ A't. pt"~$~nt .!n, u.o.t~., ll\l,..tel" ta· 1-are[Jiateted lot- no~•

t:r~l11Q.g £:~1. on vota.too•~ et~i''b¢a'b$t p~nut~, PQ~s

etc(ofl}., !Cb(l~~ eomt'ovnds ave ~g£ll3 of:f:ecti~e p:f.'lot$ot~t £tlt'lsici«em ~iuot .$J.moat th&_ ~»o rnnae ot tu.ngt ae t.hli·

.. erJDP~tt tu.t~i-~idoe bu.t a~ . ab::tnt ·one-tentlt t~ fJ.Qsgge< 61) •

())l~oottn o:oi!\p.o:wl.~e .f.#an at~ b~ ~ea; tor _tf'l$ control ~.1!

l~ e~Qt o!* oel~rft · bl$-e'b atllt (il0lt Qo~tn>l. Gl}; l:ioe,. . .

~~eently' ~tfMlQtin ttuneioi.dea ate beiac. used to ooat'*ol oof!$"Q. tsaf ~- on.<l oleo foi~- anttfee.aent rot: the: 81Bnt .

loopf!r:~ oO:tte(!j: leaf ln~at 1$. ma~ $prou6: t!wou~u.t the

oottee· ~\.'d.~·a:t."eaa of~ tiho~- or~not1P.5: ~ beim . aner.aas:tru!lY \Mled (. 001 • · . . .

·,

' ... ...

~)ltoblet1 o;;;· t~ie. ~ostelQ,e. f'~Clt?l ~~" tJJI. ot~t~

ot~.~~t!l1.!41tt bas b~ o~~hi.d' .. v~ !nttest~~t()d• ~SU.~tt~ . . . . . . . ' . - - ' : ~ . . . :

O:)wnotmdtJ mvo $.. r,~ltltll.vo·ay -~~11 half•ltt~ (~'-dey$) o~

. lrolt ,1c.~~ee ,ill th~ ·tl"ld(e3,t~1l-~)~-- -~be um6~t -,)~ --~r~o· [,, . . ·-- -. - - -. - .. - " -~ . -- - - - - - .- .

t$:A 1?ot)t-dttf,l •in tl¢tato t~b~i:'PJ ha$ bt:en f"o~ leee tiM Ot-1 ' . . ~ . . . - .

oe; at ttn t1(~lr ~Gt· aft!~; too -folt~~ tJ~'t' r~&>Qnt~Y Oil~ ad

flit.tb. b~ataa,(Gn)_., Wh~:i thcl oo•s are ·tea ts1t.b GUi?Prb•t·

1C~'Vfiell r~~·ft!O-b tJfJN ext~~S.t7"1¢f~- ~t~ed \Y~;t;h ~~t$P.11 VOrtf

little n~ount r>f t~t,l$~y·atitli ~c~ta1~e ~~ttt~ ~a t~e

1n ~he millt (~b;~t.~t 4#/tVl.)(fl/)'•

-0b(f.~-e.a1~· atr$'· ~:~on:t tools· tt:t tlodom t':ro--v r)~~ taot~n ~- th& t~~:c\-mh olf eruttobl~ o01~10~<'!a· ~~- 'b~n . :r~~lri~ 4;i"f$ted t~m~s · f:W.titlr& WOqm \lb~.ob -~ 1ttDWF

el~te oofjt. of tll~t p~_st ;t'U)Q-'"'lat1ui.t -on the etop,.. O:iaoe '

lattt a~. ~e)tu:~cb.~~~ iSt~ Wb0~q,uentlj o~~OOObo&--

V~lOVUti . O~ti~~O.t~tlo ~' beUlg iU$~~ .RtfM3~~i:f-t b.Q\,~'UfilVt: tb~~e

pQsJ.tiot«•ei• V(il:itio:ul~lV OV'~o.~.l.or1~ -hO.v• Qo~e un.ae~ - - --- - ~-~Ill .... ~ -- - . * L.rt ,...._ ~-'l-h- ,._ ......... """' -(ea)' '""i-> _ _ _

$W"~· C.rr4lit~.'~Q~ Oil 'ti4-tu xp,.:;,._,,lWF;UlC ;cjfllo'~e- ., 'i.liiuen!e· m~

r;ou.~1e ;ha~ 5 tvcona t!lJ?Qt:strum o.f· tlot!Vlty-• ·t1b1~b tltl~ t~t

beM:d;oit\l ooa no~t~~~ r~~eteJ: rJa}f ~loo. bo ~~otae:t:

o.:r?~~c:td-. I·t_ b!i\G boo~ totm4 1n O)cvera:t eatG$ t~t tl;V!\Wtns

~ .0~11'1?- 'flith au:ch cG:~ou.~cre .~. ~e$u1ted tn. a e®a~'Jt\~nt.

out b~n~ of the -~~t ·vow. feU1 r-~&~- t• bettJ~, bett.aUt!!$

ttl$ t)on-t•e i~ttlt'al t:~?ioa t~ve atao bean tttllott• m tltld$4

. (

~.-tiR.!ltiYl,;M~ tJ* .... "~ an®· ~~~tio!ii\tfi.; '!a th?\t ~~·M'' ef· tt!~Gi!r fii'!~-- · -· · ~ ... ,!~.-~JJ1~'y4,.j ..: ~- ill.i . . . ~lf·' ¥I . . • -. l . . . .. -~. . . . ~~- - ' . . '-- ... ~- .

~~-t~nt ~- ~~i;~~ ~: ~- -~'\\~~ 1#!it4~ ta~ e· ~~

~ b.~o~~ 'b9~;~ ~~tld 'to l* -~~ t)~~Ot!b• :t;t~g .. - . ~

O~~'t- C1£0A:fS.~iV~ 1l$$S' ef ~-e~i,Cl~~· -t~· tfCf$U:\tet'i :1~- f~bti' .

-· · ~eet. ~~uta:ti.~dl :~6:1116 uy -~~e-~taJJ~~~ t~ s~~b aoet.~UA~s,. · -·· ., '

God~~ft@f,f!~J t!l~~ <tU~;i:tr~l'l';;!.>ti~t o:r-~'bU\$ --~~ p?;t$01'b~,-

r,fl:_rm •~d$ Mtw$1;•· -t.o· :ttl~~- ~~i~bl~S' ~ tli~ ·'a~-. w11t . .

&fJ:~tve et v• tu;w 41lea~ t~tt d~~~o to .m~ t~io . · ·.

~~-~~ ·t!tl ii1~~ • · :®t'J~- va~!oa., :~~eov~~~ ·ov-4~i!A · . ' . '. '

:&..~ ~~bl~ QA~ifl'latficint ·a:Q~.1v~./~~l$a $nt\ ~~ aloo be5v~

es ~l~i&t~~~~($)·_ ·a~ a~ -ft~llt ~~$1£Jt.&®e- l/JU oertt~ . b~'f:~- ·-$0 . .m~ ~l$l V$p~~~. ~~- i$ -•;; a~!l.-~ .tl.:'t~'t'~~~in

t}).~O~a llav~ :b~~ el~Q>~ti ··t; ~1lG at·~~· ~t~O~\t\\fil!t . ,\

· --~~~'l~.n.: f)$'~1¢1ii~~c. tla.~t a:te -tn e~$1.1.-b u~e; 4o~<t

~Mk~~~e~bl;g -·~e~")1~te~r- w t~ e!f!t -~~! th· f!Uc~~(live t\1)1):-U.•

eations. ~h• ~f;). r~tilbol~~~- rendt~:- ·.1tfl.,,~~l.~; M.Ci- tto:U .. $

tl). -~.~~~~o ftta. c~.,!)~~lft V!b1t:b. a~ t\?t ~1~. ·QO· ttf: tbtl

t!~t-~{~)~ .. G~h$. ~t:J.tll~-~li>!b$1~~· -~l1tQ-t~ ~$. t,~ ·to Rt.$t

··&l'J;;t.;t;.S of ~!!oo~tl.~%1- ~$· ~~@tetl~ itld1~tlt:t~ -~ ai.a~t-. . . ' . ' . ·. \61''

.fiClmt abiJo:~t'1on. . ~: t~~ Gi~lt~o1llt0~t~.1 tr@Q't~ ·. · ; '•· -~.bt

~, ' .

. ' -~

. .

m~~boltGTh o.I£ tb{l Q'lmvo~a - oo~....al, it~ b1 ,$tep~1i$e

ol~~&J (>i: ~yol.¢ha1471 ~O~'O~ ~om tlW- til\ nt;:l!'l e}a 6hs•

'003~#

, . tl.ift~e:J.t 'JJ3tmti\~ · dfWit~ s, -~eriad oi:, tb.t.~~, t;$~S', btli.ld""" . .

, · . ~- ~;f'.f.ttl. ~~1etllOti·1~ _tb~ '().:!)U61J ~U;~~~hO$· ot t"tro· e.~:i:i\ -fl

. :. '.

n~liJ.Jiblfl•·

l'u 1rl..~w l)f t~. v~ ~frge ~UJ.-a~~. ~f intS~$-tliQ,\

S\t?'Oli~at::t.~n.~ ·6f' 'f!ho ~~).O't$~tll~t n k~~~~~~ ~·t ·tb~$.%l ..

~rn.v~~.,~~-1 ·\u .. ~triiom o~tttur~1 !.@ oW1o~ ~poritmt to . .

~n~l!r:'tt tb~i» e~t.'inuea oot~ ~~w., A e«\~~lj;eel do~Mtwn. '

· f10t~tr~ ·eo,;.· 11:1ih~ t~ibt\~,1 ~ fil,~: tt .. i.9h00fltin de~tvatt.,~e"~

~~t:td on ·~tlri.t piX)~ ~; Ghelc.on\~9.1-,.. wh!ob J,tt .v~•bl11 !\·;)A'JiC""b"l'f0i t·,._., o·· tt""G!i'~ .4~_-h •.. n.. __ .&-·.~:"""~ .. ~'·"" a't.i_· .• e~_.~:'i!i. il'>;_ .• ·~_m:_•. ·

.... t ~-· ~ . ~ '*'. . .... Q."" ··f""'V"""4.V ~;;;.~:J 4.-p ~101: ollo.~'i¥~•;tr,:,~:!l:

\

U. V. OR· MICRO-ORGS.

U. V. OR · MICRO -ORGS.

( ASnOOH )n . \ -

J. U. V. OR .

. ·· MCCRO-ORGS .

. Sn02

. . . (70 '71) Fish and his co-workers ' · have si1own that

· tribut:rl tins nre :1le·ta bolised 1!!,. vi tr.o and 1!1 vivo in

mammals to di- and monobut~rl tins. via ca.rbon-hydrozylated

:Lntci.:·medLitea. ~·Un.gal degradatioH o:J:' T .:a.1.0. to di- and

:Jonobl,ltylt:Lns by Conioph()ra· cerebel~ and Polystictus

.!, J

k~~ ·!n~ mleo beet$, revo~ted :tt!JOflAtlU(?~)~· · Unliit~ m~rourt Qo~pot.tr.ill$,. ther$ le D.o ·svit\~nee of

@etb$l~itJ.on of or(fil;uot~, o~ aooa~t ot t.hei:e laCtt o~

~oou~tO..ntio~ $.-'14 ~~a4y brea?"Jiown to: ·n.c'n to;Uo .. il'.,ot-gar-1-io

tiA ootJpoW'.ltie;: ~ina tbeae -~· qu.:tt,te flOooirtable. f:roa

~iro~',)n~l. ¢oueidettQ~tlon;a(:6l,) .;.

'i:h~ b:tologi~ aot!vtw o£ orga,'"a.otin.e ba$ b«'n:

<YJtt.mtttiv•~ (&t~Id1.,d. and it ll\l$ ·been. toona: tl1-J.t t$t~ox•tJt.oo·· . . .

ti~flj ar{l!) l"bt ~tologiQQliS' aotl ve W\i~~sa they ~lYe deaJtt"KlG~tl

to t.t.'io~~Rlt)tin. det'i~t1V0$ UAde~ !1\ ShVJ! anf.t ttrtl ~~tJ;'A

co.~.:~ttiGns•: ~he b1ologica.:t e.ct.1Vits ~nttem .o~ the tr,tw.

or~.rAl)'t1n.al ll$$P.\Ky· is· no;~ w~ll. kOOwu.~; \-1i th: th~ com,pouwls . . . . .

abowtnB a; mtWlS!ed. . ~!.H.ttaie~ 6.ep$ad~11.~-~ ·tll;oco~~ t.o the

na~~'lW<t (J:f i1 ~QU!)j: bt\t it'~lo.t1vely in&,i:t)0Udcn:t Qf tbe aaiQni()

·nid1eal<73>., x •. 2h!G: r~ be ~a~ie~ t'f.$ £oildW$(4al •·

. In.sectf:!

~$

~~ n~~t~v:e b$ete~ ·

Gtii", :poei tive bloterm.. fteb1_· ·.~ . f . . . , ~Bit eollue.em

!111\iJh, tUP...gi~ ~~:uuoca

~'"it" .t! .• a~

M~

:st

. ., .. '

~i.i!l ~,a i.ll~l-"rA~~<l .is~ J>t~. ~a· t14Mcb eb)us, tb0 iile:>ena•nee

Gt tilt;~ .bit>ltJ~tt,~ · aat1rtey of: ·tbo tt·i~~alk;fl'li:tn acetate&.

o~ th.~ nat~ Q'$; th• U'l"'fUl<J:i ~li~ou~ . f0r ~i1..6t.1$· ~pGoi~e~

fh~t. lflt~tr· ~1tltl Ct)::\tmWld• ·-.~a able· t~ ~bit;· . . (V~1 . . .

o~:tfitJh®d~1. ~1.~1:\ti.'{f$. -~bont}hGlfN:18it~~n · ~- and !t .Mt!J

b"~ etU€Ul~O~et ·by ~11 :~nit tiu01t~~ (?I) . tbati tho~ "b!~)..ritJ!.c«l ~ct1V'i- t~t'tom may be <l~IiEWJeut. ·U;~~ the

. ott~ctlV~fJI$~ O£ · tb$~ ib.te:t't~eytton nt Wl aoti'\70 :alt& ¢).~

~i:toe' ~-i.Oh U~vnlVt;f!. C1)~rd1Jldti®' tQ_ ~~rtnit.!; 00~ [',U)~(]$_.

~ht~ ouct ct\~1~ .. -t~At~o of the: b~n.n1ma $l ti¢f~ M the - ; : .'

. F~ett~t.n bp.n b~j&n 'tbi~. ~bae~t ,(}f oM~it1eratsl~' o.tt&1.ti0n.

~~cttltiY ~ilitl 1t btl$ beoo. .atJl3f!OOtet1 tt\at tho ~?Jilt Oti'G~bl.e

b.~1!\B ~1 f;~"f$ f1Q1.* t~~a:~l¢t~~ ()A ~:t.'fjt&~ npi;t?~ tcr tte

:r;rjj. . ttl~ •SB &Ad it~1~ole# ...:nu ~oups(4e}., · ,· ' -

· ·. · ~he- ~Ub~1ob~ ~$po~~s ~~•ltu ·om:ttin. too iAtti&l

obs~tion or 31el~:~~~ Ul1ttt,~rttw.ce _of ·t~ mtt~0 of . . . ' . . . " ~ . .

ani~liio nonien oi~ tllf.) trio~"~ttn ·fu.f>l.~:oute te t.bi) ovev

o.lt b,,.olot#nat ~Aot:t:w!U~Y..~. Q!.thl~uh ti1'0~ ~- ·O$Ct).f91~

unwb~M~~mtat ~D(fi'1~ of «~~dw~•·-~oltw. hue rs~rt-

. ttct rn1a~ tbe t\5~~- (~up {))t~· ta· tm) •tlte *'t b~fle{1i.ooi . .

r0~~1on t-.o• ·at e&flt .l'.:ito-~·ja1 .t't:· ~- rot ®t1lcv \.'fUlt . . . .

·the r~-- ~~- itc but ·t·htt latt.., ~' b$ s!gn1f:t.oontl:(

i~lv~~ ~Jl! batlt1WO~tn.3 tfl~.· biQeld~ ti;~ tbt) ~enetivtt $it,76> •

n~ hft,~a {;l<)a.if:~ltlt th~· x ·~t~ ·!t~ au(th 1'4 way t~t the ialtab.Uitu

.,--.

,• \',.

.. . ,'

·1···.· .. ~ •. ·. j .

hctivity

. . , . . . . Mammals

ll . . ~ '

~ . ~ . ~. '

• ' ~ r.

r . ' . . .

·.Insects ,X\ .. . . .

. . .. . '

I J

I' Me Et Pr .·Bu

r ·• ·--~~~~·---··---· -···-.R~:in_·_R~2-St~1.0~o~£-?~~~-!_!~·~-M~~ it ••• -~ ~h, ............ -

t ' . -

/ ._Qi1:= ~~

Qf .ttw ~~ei:tl~~ F,:t'(Stitl.lt t.to:~t~H,ttld -e _mtlbGtant~l;b' ~•d·

It$ p~pe~oa a t• t~ttmwei$ de~i~tivee ~rh!()b, ~w b~t$$1 ft'Wg1a!t'At n.et:tvitit;~ ttGnin~ ~iQt &ll~troYintJ t~,

tllthounb ·"ijntl;)!~· tit~ oon~nt- ~ ~u,ott ~Mll~if .tb)lil. tl'l$ C(}r.Jll~ . ' . (

ot.tll. tJV~t.it';4 u~tnt:: ¢~-~~tmt!Vettst .

ffill'ltette ~· aa,,(77') ~tl~)o~~d thnt~ in- ~~-bQl.1a;t~ ~~~ ~-n b~ns~ ~'® a{!a$w.t !l:!lit~h;tkp ... ~·ft~·~ ~ tor~, S~Jl:P:mr::t•. aqftl on. oett9~J tm.ti gaqg,•fJtl~ .. ~~ill oo. ~~ --·~-' ~~ ~-~l'-1!4!1- . -- - --·--<Z~- --~ ____ .) -- . ~. ·- ------ .

-h~e.t, ttw; ti.~ ®P~t~:c~~ (a:;) ~n3(fJll~.... ~!!$atl·~>~ $td

(~~.SUGlar)~- (~"i~~~~l\Or~+(l~~~ WE1lt'G $.~~C~$~ to, tt'~Vb~l•, tim. $f!R\tat~-~ t~1.ph,'?ltbl Qtl\f!.d$ at;ttt tittffi.-!1!t~!:tr1nr .

eydt1~1!~~ tAit eorge:~Mng .. d()JJ•G• :~i1o.td tfit$.~ ·fJD.· f}Gtot~e.it

e~l~~ ana c1t~~t-betlt.~ &l$1) r.>~:ove-l t~t ·tnt oGrlplox-Q~ &\Ve

.:ir.l$ld t,t;t~ttt"1M~ttn+

l.lttt wvq5l *i1bi.;,Jretom -~ fou~ . ~o.ta.Jl1d~~blU ~,w~.v

: t00$1t'o~1~ttt~J ~!J e:~tf.lr~t~tll Qh~lnted ~f)o®~~~• J:tl ~~j ' .

·'tw. . ,t>l', 1ft""'- "·rfi ,;;Ja· "i>.-1;. '}~,.. f'" l"l~tU...,ffl.if:...~·r.i6; ·'"'·'t.."l .>i;. - ~!l""'; . ·. -~~~""il< ,~,-,y~~'·.A~t.-. ~i~Y ·-"""S";:nvv ..... ~,~..,nv~t 5-•&.~.Q~ 0.~~~~$ ~n.

i:A~~olecqtm;lN.· ®~1'di.Z\$t~ M.eth;tl€01i10$t~_l. 11~

tthn.wtl tb~ ~ow~i1t a.ot1~t;y tuoa,··$ oolltl$\11did!t(fa) •. _ ~!lis .1.$· . '

'f.)l'\)~b'ld aua- to. ·a tt~t!lltl~tl t.fl1ii~r,.ey fo~ thts ~m~~u 'ttt . . .

~t-ok tnf) ~c.tiv~ ~-te~ on tb~ ~l !)trote:tfllt ~.th~r tlm t() ~· ~'~~ourit Va:t•iattton _in tbt1 Mee ot ~~~~tif)ll. o,t

'· . '

th~ c~~OtlU® t-o ltt:t~oh tt~~ *tw ~tam~ $ot~~~'- ~~mob rula, &:J•~JOt:•l'::/Jr~(?D} blv~ totm.d t•t a ~ll~ ~t~~l~CUU:x-.t,v

oht~nt~(!; tr-fbu;tz.lJ..titi d.1nlcylruri~nlkt")2tide !e. t:l~)::Jl"'~i:.J(}.te.l,y

(:r~f}ht tJn~:la· leoa tflX:i() o:t.'nl::tsr· to oioe tf'1ln i?~.I}.:~: •. ~Jl) ~.}01.

it .fft'H.U.tl Q~Jr~ar t'hn"b tflo tot.ta;tt~l ot" the t.rialf:yltlrJ. oo:.:'l ...

~-){}Ut\~_o_, r~~n?t;, r.:n.Y' be ~;,r:~ t1t'IJ.t de;;etldcnt au the nattt1"e

erf ~~ nrotlZ~, ~.f tb.e· ~3: {.3r.Jnp :5..-tl a. liGl:.\tM1. -ut~2.oh ~l f..')l7D a

Ff!_·'fl.m_·, .ts. '_IlL_·_.- sm.~·l'~~tr ~ - Ji~.Nf'~ .

\ '

""""' - ,e,,..._ ,...o n..u~ -"""""i!.'>'ih ·•<t;v;.\l .,.....,:;:; "''"'"""··~~~-;,.n,~_'t ~ • .,.,;..1..,..., · ~ ~M> tf:;J,r,;vt) ":zib ~o~<'"'~.:;...ol.~~;?.v v' !.M • .;,t1 u\•4"!,.-~.;...v~ .. JI-4a t;i'l,l'f.;U:f.:">:n,.,. 0il>

tbo o~oa.not~n c.J.-Cll~a~~tmt1.oi:l ~Qnpoutl(1S r(;2(;ol'tod lln oo~a.s~

{lt;~blfJ d~-~~t, ~o· i~«"~J;x~vf;f) ~tiu:6Y · o£ th~il?· t?Aoot®-1 'l)l?>tJ ....

'iJ~rtic.s· l1'1.V~- be~ ot:ii.bJJ~.O:hod so :fi)j!• l~ !;o o~l:i~Ve\.~ toot

th~ 0o""O~~itiJr.iAt:ll.o!i -of l:~G'lf'..tia t~i t1.tl ~;ou!Ltl o~ll~~"'~l1Y t!'~UQ8

j. t~ ttmnttolt~ f..':l~t)p.tt¥#.Jieu• ;bUt: t~1·o al?~ o~:t."tr~h\ ~~~;ot'te

_.,..j,~ t:¥'""'- ~,..,~--"f'\~()'!'"i!t~(;?G,.,~~,,01). ... t'l'l'\""' .Pot'!l'l;ri!'t ~A".,.a __ -. trt> b_"Ztf'~~--.,._. r,>---P ill;,'"''""' 'SJ'~,li. u .... ~~~, ~::_..., ... ~'~,~':;,'41!J . · !~' fi{J"~ .... t.Q· • ~/~.'V~J__,~~ ~~:~~~· .;.JJ~ .:~;k.::.tv

ot"'~~~ot:it1 ~ord~l~l~ltilO'n &otll)otm.do o;o~o~J)l9 eJ!-l:l oov® ~e

{1t)pl!:lnl1eneo: ti!tl t;.h~ tl;;"l'ttlve ~f· -oo~or~'i!f.;.at;~d'~ .l.1:tJm¥1"~ but ~­

£~, .l1.1o~:rt of et~t~~~of:EJnt 1-;'tti.bl1oi1,~~'i da;;~. f;J;e-.;r~nt ·uG t'o d~~t1

"" .;!1J:"".,;;'~ r' ~ """" ..,,..,~,.., ,.,_,~,~ ..,..,,. ,.,.,if\ .:.t..-t~ ,.,.,~c.,.,..t~ ,.,.& ""'"'-''-'"""il'<l4 .,,_..,.t. ~- --~..... ...., ~~ P:,~,\1:~~.:.~-t~.t.J;o y:,;.,J.[A~~~-=~·v ... ~~.~~ v~ ;f;J_itAv· ~"'----~~·1\t -~)~ v~;.p;-,~..~~u.~v;t.:QtA. Q,.._

"tlbCJ t.in \·1ith !"eo.~ct to thoiv antif'~·uu~l p~ooevt1~1.r.. t1~re~

o«:i~:ft. V'¢7}JJ ';?d:ttl~ W!:)l:it ha(J b~~t"!i. UljAf.$ a1- fnt-t :~t~ ~&i.V:l:1& ,Qb:2U.t

·~ihe muitablli~:1:t:l of c"ll"Ga;:~ot:i.n poo-t~1,o:tci$s ·oot!~~ lrJGQl 0\J~i-- .

ti~flS•: il~~~t':l~'- ·;;_.'~ <i.el0~~~1eei. to \.-iUuO:!~CiJ:te- the C:Utrlft.\n[}:i;J •. ·S't/U.JiflO

.,:f .none ot'tJf'J:J.t~t:~.t\ e~·tr~'~:l?.oot~~ot~ cH·t.l!J·~W'id~:' t:~t~::tn~t eJ~~~»

i01'A'1~ente };.lont ptltb~f.lei·1iQ atld \"JOt:ttl ::.."ottU:lS j}OOJ1 1J!!"~l~r

'l.tr:t. ot:r1t~. """"'·'l.-l.:i>i'··'tn,..,\'. •'l'~ 't:h.-. l">f'ln'::;'it£~ """"!' ,lli.r,,<g,·fi. ,...f-.'f(~>il:i"~A £if1"-~'tl!""i <l_''!fi ~ ~ ~' .. ~-c.:~~;f ... :1't"Ji..r. ... - --~~~ ..... ~.NJh..l -~;;,._~~.;;.,..~ ':;.:#~ lL~tr..VU -~..;.r-tJ,.,..,~~~ ·"M~-'.l~· -~~

:stl'\m stt.t&:!Leo; \le !\ut~Jitled, ·~o ~t~tdy the of~e~ of b~Gse

(}(!inocn.n~~a' 't;r,} tr~nt;rol" fj£ bV$~ 'le~f tlJ)Ot ' a.1 ~'i)t') vi' ~loe

r:\tld t'U.Jod ¢1~to~iorat1o.o.. /~:lolm tJllth th~ nut:!ft~~l p-rt'joe,; ....

t1eff• n~J~~~:.-:tnii!V.., ~~,:~tl~ol of 'f\liCU:lt ;";atl\m~--~~"ll .. ~. :f'tt.n,P'!• ·it t>:!A!t . ~ ,·~ -• ~'~---- . ,.,J v .... - ~- _,_ .. / ..... \...: ... -~-~ .. -·' . -;;_.);1. --~~- .. -. u~ 'll' - ""'

c~ler:>- ~<~Xt('.Jlfle(i- t;) ·firtttc19' tl:4~ ·pbyi;o.to~i.o l'.l:'1t'iA~ of th~~,<t

e;:t"{jfU'k-,.t'2,rl- oo~ot"'d.::J~:tt:it/':1 on oor:1poun.d a •·

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~- test Cot'l~oonaa &"1t,)lo;v$\ d:t.trf,l~ ~ll~ !tl~(l)$t1c;.a.t·ion

tte:~$ t~13l~~1: ~nd d,~oP.g&Mttr~. ~{ll,itpl.e~~s~ i1?; t!~ li~$ like

t'!1J~h:Jr~en~;, ftiph~1 ot~~'#A~ n.~& ~1., bi~l·p~Jql.

~Vd;t'i~t1t:u!.¢ :mota •. 1J0'fj~ouaa Q{>-(;~a.t~;o,:ftng o~:t!:l. the l!~;

tjl?.AlW:t bill~·v~ol.Ul hvd$'~oc:!lt?;) ac.te i.e ·e~:eh ooe· ~b~1.1

t\'TO tt~ovt~.;;-otin ooi~tiee ~o beint; ®~~tl!ootei w $

Qi,:.f#Sl.(b' li(~ ·f:llJl.001llQ• It .tla~ e~~atet!} tl'*t St.~cb Qo~:t)Otln.t!

obbtild ~:M:h.1 O!t b$'tt$~ t"aQZ:!to~1 .. a ~tJerttee t~n thli otbe~

d~&l:Jiot.!n. oo-.ot>lt~~:iG:l oor::11oun~a ·an~ra fdlt)· t-:tt .are;anotm

t'¢iOt1 1~ OO~!'StMted 'tf1 a "i~~··· lt~~ QOlfi¢tll~*' ~a-1' :'

tut~,test oi ettOh O:J.'f~U\lti.~ OO~iMti~t\ etit:1$.!0WZi4S· 1'1~$ 1n

:ttaa ~~~t,.: ·th~h M~o~1.1i'uQtian oocrpOI4tlde haft o~:taitdeab>ly

lowe-~ ti.n c~t~t,· t~ tit.'}r:t1Q)XJ.i$ll¥ ':fmpo~~t :8~®1\fi

fj1.teltattWfJ aurvfl,Ve ':tn~~Jo~t~ ·t~•t.: tor oont~ol1.1M ~l ~i:i~~~.~ ~:f r>~t'ait~; ci~,.~~ot~n. · ~~to:L~~aa· 'Ot\1 bt?J·

(1.~dlio::.ble 'for tb~!~ h1Qh .t£et:ft!~~$·s et • t1.tl0b ~0ti$r . . . . . {eg ~2\'

crnJ.~$1tt~t6n -tl1A1"~ tbi ·r)t'eviciU~la · u•a tiom»~~ tur~~bln&m '. r.:t,r00v~t.)~~· t!1$~ bieH!~graii:it1ou. ~tt\.·tt.~~ ·te ·ou'l)b ~r.e t\l'f~~b1e·

t~J\Yi <»~P~ fm!J:ioidett t:rt:)tl tbt~· ~oint of ~V1~~··1ct~a1

· ·oor~M·\!JiX\t~.on$~. ~:r~~e;t:n~• ~t \·:aEf b1;liev~d trAt tb~ st1u6lr

· · (}t.· 2~t~:taat· ta:ope~;;t$5 of· "~~,~ot:u1.· eJb~o~na;tlon otp~)r,tm.dtll

(my b~ of O.?naid.tu~bl.t), i."a.t";tieet fi.a:f! tbo oow.t of aat'iO!i! tural

· t.\tm'!'iog:t1ooaQ · t®?tielUut'ily- ®Clt<lf Ii:tdt.n f1~d oonatti~tUJ

\Jb~~~· t~ ~t.~a of' r?tciit .~rt.)~$¢bi~ acrent~'" -~-..\!! t.wa.4~11 L~o:tt~af1!tl[to. ·

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ultm:b \Ua~Q~t!>a ~~ Gt' ~a.t .Qen(}l&-'JU~n.ee to .~n ·$$

they onuo~ ooro.t\{)0 to plantfil a:~id, vl·ntlt f?VOC!ac'tH~, Hu.~ ~ee

is ·~}ri~iJ,y d.epenel.ent. on E#kint prod·Q.ot;s i'o-'V' its ~~otsW€h

:~lm:l\t tl!soaee cnn tru.~e the· tlU~ere.tle~ b&"t·;;esn ·a tl&pf)J :life

ru2t\ fb. ltfe: ham1t~(t; tr;J· hu..~o.t' o~ ean· ev$n. reovat ~.n 4eat.b

t:rom sta~t!1.oo. ~ll<l .ao~th ft-o.!'j otan-et~~on of -~ !;'!U.'l!i\t:er

otll!ou I~:U~h in ~fJ.i.Mj anfl .. f;Jti\llb Of th0 bunrJor o:£ the

:u.!ld~tof<!d Oil.l.io~ l;tv.lrig m tho u.nderd~velor!ed oountrteli

to~ are t'lr.7lrb1¢ ~A:iftlc.~ wt tbe ootAa.e;~u.enee!\1 of ·o-1~

d1 ~as"s• .r~~ countri.:-oe, f(Jber&. food ia. ar;ne.t'nll»' .. 0:1en,t!£t4, . .

'trl:nnt d:l.eG$el~~ r.u:o ~~.oo iopmJt~t· boanuoa tiw.,v oa.ttO.a 60Q-~ . . ' ~ ' .

ni.«) lomsea to cr:~y~:o-ra ®!t !t~0~9~ &~1o~e of .r;~dootttt for

COtl~'..ll~~-•· !'t or.W: d.f>etrcq th" b&auey· .o.f ·ths ~t,'Vit~00!7!en't4 bt

dGtcrAtlinC v·l~t~ ~ro'U.Od ho:!1~0t ·aloil!:! atr$1!lta; m;n I'er~s. -41.n~ - ... ' . -

!t,\ fOl!O~t.e!li

·:llntlit tlia~1:se~ r,;.ay .l,..,tn~;t tb.e ~in(1B· of ~l~anter thQt

onu fjt~W in M orca Gl"' .in an~ eut11Je ~t:>oot:w iJS' ueot!'oy1ng . ' ' '

· a.1l, l)l:J):\tS Gt. C~Ed,n O!)OO.ias th.-~t Ul:"'e eJt'tft::lelq t3UGQ$f)tible

. to -~ puvt;iat\lor d~G~H.f.t~e; aw ~gem9lif!od by tbe A':~e~ioan

oh¢letnutt ::.}.~.ah wae Grul1Ml3tte0 :tn. iiorth ~m~r!~ &a a timber

u1o1~.!nt1 tr.~e b1 th-o choatnut. bli6ht d1~ •. ~~· ~~ tw.::t al$G

~"~totourl~ly· affeot tldvorael;V. tho ii.i.5"r'lout turol 1~:tl~tr1oe , • , I '. • • ,

(Ul£.1 t~ l.trV~l o£ en.t">loytaou.t. .tr.l; . O:ti a:rea-. 'ito Ot'~tl?Ol plont I, ' • ~

di.f!)OO.Seq; tb~ oro.at.'iot~ o.f' n~ne-r :t~t1Llf,ltric6 t~tJ the: d.G'V~ap­

mcnt at n~1 t}oet!.o.id~oj, oa.o!lioo~!eQ ~. oetborta. are ·t>t;qu.ir~a •.

~h$ e~.?~nt11tt.trd\ to ·~.bts $ld r.r-~ ooo:u&lt to bUl1ooo· ()£

dollta"~ pe.J? yeQI! tot'. tl:l()· ~~1.tl, l.t'l J:nd~~lf t!le eultltf$;1.. otrop

loaeew due to pl~nt 'H:seltlm~e ood. per.~ts l'lo.V(} b~~1. ~artl0nt$d.

to thG ta.:mG of f:J• 12;000 m!.lltoos .in 1001 pt~too le~l (er3) ., . '

Z~o!;l. the ~1$ &lf~ @f elv1li,.at1t.!on, oou hQ$ b~~

~tJJ~ to rwevent ru:ad <!.til'& oerttt!i?. ~?lent &~sbs -~~ ua1.ng

the ftjtlgio:tda?>e in o:c~1o fotm• DaoulG :~\tl t!tlf> ~.oot®t civitl-..

cation ~..ntl f,nun6 {)ttt tb.~ tllf:t:CI'cttv1't1 o-f o&t!t'ain mu:tH:'ltrlalm

. a(;n.i,nat pl!!tUt d.i~l#.O~a at.~<i vest~• ~:b..~e nr~ voeon\0· ·ol: th•

t~;e~ of £ur.~atc.tde.CJ ·.by tht! G!'M~~ oo~ ibman:a~ :il~~ple.e of

Mll1~lt ¥:~1idie like 1Ji!t~~t'ce·. aud nociiin.O.t tre~ ~M'l~t!; etf plt:.l!l't

disea~n o.ua p~ato tU.lfi th$1:t~ ie;:JOI't6l(lO~h. !~ uuvgfila' e {134) . .

V:t'ket\YtU'Ve:~ ·. · b~O o:t\•l1}.p, \·i$ . .imd tr~,fet;MG~Hl t~. t1~

· dtt~60int;• plru:::b ftn:if)ltiotu; ~ e"d ·~tttments 'tll~;:Jtueb

the twt9, ot a;~oe ~t;'ritu..a- ::Lik~ oowte uri~•· Gh~ \~ltter·

oill, Vid.iilt~: ~~-i~Alna ~)J2),, uua.m, eoe~!!i, ~to-.

AlOntJ With the JA0~1BiHS Rte of a.,.~\.VlnG 'OGDUla­

t~.!Jflj 1ntoneive resea~h 1n ttJe :fit~lt1 ot olnnt ·oret,aot.ion.

htta· b~.a .sotns on th.tv.'J~h ~~u.t th~ tlor.l<l!!i· .&a. a 1J(:ls;.J:l if,.

\l"~:r1~»tls ty;ry.~et O<f fQ.nc;tto,.~~t! a~ ~$1!t;G P~Ptl.Ped; ~ toe!~

fnr~oi~l, t.olt!ooloG'i.<lt!l•t I?h~ot~#C ®d trthe:tii :p110t)dllt1e$

ore b$::!,ne ut;udi~:tt •. na,:td pr.oare$9 ~in 't~b __ <l t~\il:cde;(";>. fif :f~~

.<:;i<.l$~'h hotfOV~VJ- t"Jit~ tlni!e Onl.$ fltte~ the 13t;,b OOiltUptJ' WB1fi11

beoauoe or the ·e3.:Clv'al1P~1ent .il1 the {d"..O\'Jledae: .o._f ¢ret::J1etru . .

)

~.ti th tbo ~eev0lofjtrn~;.t of a large iltf'lb~ £unot·ot!tee

of tU.v-~r;~r aMn!cnfl .c~~l~a ·;ttlro ·aulobtw9, CI?Jt~P~:t• ~oi~~~.

tJOinotWt o!1on.ol; :b(')'t~;:o~o1&• o&antllin tm~ .. otu~ro., ot'Q14f¥j• ·

ru~-tsu111'ls f~-c t ne t-ast t~· deo~4oa• Mva e8t.b'liah~z\ tnf>n...,

e~1;."';reo ta.s • t1ioti!:lot: olaso of. £tm$io1.tl~s.. '~71ttain OI'~""

nG!tO:Llio$,., o~a&fi;}~e~OU!"itits au<l. ocgQr:~et!ns !ln,ve >trori} hteh

-~sng1ot!dal aot 1'\iity. Or~;;iA'lOVm~r!o.lo nav~ ·boeta t1:sou o~otJ$

;~:ot&· ~t~).G t~. aoe~e tt') ~rot~at thefj• $rfJfl tr.moal ~ttec1t•

t~"cmtse t:l£ th--air etlJ.t:'Ome to;~;:t.e!:ty... t!l$1r uoe £or eJ)!.t'ayi~s

tho f.ol;t~o. O"•Ui..,d· aert b~ eno!;llet~gelt;. aa.~~~iuc 'tlle a0st1

tr~:~.iz-,*'nt nloo~ oood, ~1.j~1cy ·is tlStilo:t,ttere4 ~ be on.a~ .01!? th•

!:\\1..-:aJ!'de ~--i:l t.lle use oz .01J~~~O:!lGl.~ou.-cj.QJ.n.. .!\.ft()t- 't!oetl~ tt~ . . (t''lt;%

.t:)tllf.J.h1~rc·J.al ,VJse! mte ·ttn: l·~b~rt~.t.ory, Crooier -eM Dt.ag,~tt$ '<JJ • 1

t..,un.a tbct ebn~~tl C)elic.ttu~!.t!.rnlf*· t3'v1o''!l of· t~~~o-u.ey 't?oionn­

ing, Will~ er.a)f)n in Q()~ra~l· e~4 ·lote· t~t~d Q:i~h oraan?•

!~~our:t~'l.-9 ·ot ~aoorr~.~d .. R;tes.· ~bfJ. et:~ot :i·e a c~otttJ·N­

·iatic hyp$t"tllo1;tl:W o~ t-I:e: ·roRltgJ nne! ocl.eopt11e~ ot oe»onl

- a~t111~1ga"'. ~a...,(a6)· tr.:.dG an. ·ta\:at~~loal; atua,v of th~ tw:vev•

, trovf!,y ;in~uoOO. -~Jl ar;.tee qy ne\v 10~l:t?ti~~f)d . Q~~G~M ·( ~tl'Wl

t-:wreu.:~·J vbouphnt~) .9 Be ·e!:'::.¢r;ed: tbat. tn~ :plll~:;;tJ~1a. t.md!lre;o

aitt®t~eiVG· tfl!Oltl!Ablg t.!Jl{l ·davel.opetl.ir:t'~'tit~ oorootions

nnd lobe~,, CL.'ld itl i;bo G}71Ci~ oer!steta. · of the plt.J.mtaet ()~ll

(tt~.riei~n ·1a inh1.b1toct D£~Jr1 olrtma eti.l{W[l;~~on~ oft tbr1 e:d .. ~t·

·in11 cuila. ·ti!?:Jtoo. ~11~eo~ t~he. e~ll.~ of. tt)~ bl~ot't?:o:oh!ed

· 0rf',.vlo ~<:t~ti0 rl\ll t~l~(doa;te~ Qot\:t&:3;atn.;;; t:lU.01 .. eJ. ra..~~.

~?rom t:tl.~onuolei ~ oolJ~l.r.J;tti gi,c-~t t~ool~ot ·t~.na theA ·ntinlbo:r

or Obr~-Mo!3mes ~n tt~ene oftl&.~:J.. O$:~$di\.o.;,; hlOO• . .

Ht3ceut1;j, tho e~~~&~l uo'S ()f o-F'~loo~c-ut'Lal•

funa;tci,f:e.0 to Gre:tdt)ol:!Jf l~O:tlinG tili$'!;t {)l"OUAU.e d,UJ) to ~gil

t:<~.attlali~ and :onyooto&io1t!l.~e<2» •. I~s powl~~ out t)wrl!~~-~ tbe tirst it~veot:iun't16lt

!a t~ 'fi.6le1 of' ~:nlttlf®@1l.l a.otivtty of ov&.~~o.t1Jl o&.Jpot~$

. t*>lJ& et1%%•tetl out b$: ·inr:l d~~ t.~0~. tlti!.r1 ,~ut~ :t~n. 1n.. ~J~4 ( 13) 9

lt wncJ iot:!fiet by t~~ t~~t on]$· t~o~ottn (:f~;:;n~\.1 ileri:"!*­

\Jn'(rt'tto~ ~e tho ~~erint. f'(lnt;;ri.oieea# ·fhs oo~G .of .ths

ort!fl&lfc, .f3t~-0ttp a P.~pc~ett ·to be-$)£ <1e(l'1tlltv~: .:!Clport;;ar~ce. 1A

cO·ont~ot ttl tflG l\~ttwe of ·tM. fj!)~th ~':>~ ~; t:i~(}tJ. d'd

not n.ryp:f~oo.inb~ ~ueno~ nota v.tt-y·o;· il. .n:um:b0~ of 't$t~_.,, i '·

di.-.; onGl ~H)r!l)onbot:.~)~u.ttld t'):t'gn~Qt.!n; OO.r:l£JlliuU~a 1\:',Ge~ ~~ted:

ond aU oho.w~ tte~!J :S,;;1·tiJ1~ ~n" t~~r!C.ot!ooll,V no ootin~,

oorlutlt't'}rl to 't~iot>~K¥;)t~a n(Jl'iVati:voo-. . . ·· .. .. . . .. . \ £9) n .· . . \Sf) ·;• . . (52) . . . .

&:.::edt~or otJ\d li$!te~ ., 'UStt-'tel 11 r:;,u:r:o ~ ' ' . t'~'li'l$.VJ t.:l~t~veote·t~)n ·31 tlQ.V6 ft1.1t:r» att\dletl t110l ao.tif"'llr~.._l

®tiv!ty o~· t~ior~not:!n d~t;1;.wtiv.~a t-8a!Mt t:fj~e· o1mnt

Batb:.JGEUJ.iO ~Jl!Bi•' /

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rnur~r be~t .t\M ggr:t~,.a !!!J&!t~ or~ tn!l~r-.:9.ao eto~ .~a eooe ~t

tl~ Jh~aait!;o: .f\!.f:'..Cfi.._... ·e~~t '1n tho fi~l:t'!~t. ~~- nt- o~f!sent

au.cnt.rouftl.llQ oe;t~t:ro~Ll'~d b9· ~~~@ CO~'\lpomae: on ~ ~i1mtJe-Po1•1

e~1••·

oo oblicete rm:t~t!Jit1c fun;s! h~ve been, oe.rrt~a. out by ~ 't. r1 · -t""') · .

11~~1\ffl 1 t74l4 q-,. :Z:fhl!l~el\·OO ·.; ~be fol\laC;J~' f(}U!~\) ~lat t}•~-"""'"""""..,.~.· ·. fi'" ~~i.tl!)fb .i•'!'t: t~.P.t.._I!!J ~ftE:l(}tiV~'i."'.'~" ''i<'~"'~'Yft~~fi:lA,- 'bti· ~~ '~ ·~· v" ' . ~ ·~· . . . -~•a w~. t.Y~,'~,JU.. 9~~ ,..,

tr1butylt1l~ th~ ··tq t)llitJ.ert.bv'lt~~n ~· tr.i~!)Jltm cQ!nt?Omlde,. " ' I

t~~ia!t ~d$JW' olldew -~ ou0ttltev- the ·t1"'i~tqT:tt:1n d•t'! ~-,. • • : f • •

ttva •mH.'$l'tiSd to be·. ?\!t~-n~'}J;s: ~t.1V$: t1~0sl'e$e t~ t:~r1'but~l

a!ll:1. triuentyttm d$r1:v4t:L.iWea we-r~ th~ Gluot -~.ffoo~iv0 o~-i . - • ...

~~}otnda -~~~. !)Owti~~~ .. milt:te~ _•)!."1 bn:Vl·$1 o~ ·t~~, 'tl"!f?~lt&l

m1.d trth~:(Yl tin (.\~"1vat1v ee Qsa~'t tv~Pl~ o~.l.Gt£1~

ln th~ r~1~1rn~t:r1oo1 t:ti.Qlkylt~;~ ~e::i.tJ~a t~ ~~Yl

nnd 'but~.,:t ~f)mu .. nt.«'1.ds- r;M~tat.l~ nt>o t.ba 1:::--~~t et;t~~ <!e~!~t! V$St

t~1o. nl?~seuo~ e:t. ·t.r~e$. ~1e..r~!etila;~· e~"UPfl· ara_ f~¢'t\ th\1' n.ecee&.A!'J

Ot!~;d1t1en .fQ.rt W.:rh Ot¢;t~Vit1• ~hi$ t~oct.r.le ~,.,j!(~ ~vh~l

unm~~EGt:&!.~\ ·tr!allcyl t!n a.c~~t~· ~re teat«i h.4l} • 1·\rot1 ! ' I •

1JhnSJe !llv~at!~t.ton0 o:t Viul de5'~ ~er!~ 1QiJ::t-dJ~tdat•• it -.s

obt1e:ludsd. tMt OO!'Y.JQ: tt1e t~!"ia\kyl,ti.~ noottate:s~ £:S~imtm

tttr.f.:1$ in ·ttl& Bl~l t::~li'ot~~ r~~le!W of th-e· ilatm.~ of the

~atl!vidw ~woup.

He-b,";fWd~J~. tb!t tz:~t1'l t!a e.~(IOUl¥1~. tVJl.•;>~~e>ltl tlr4

ootttcte ood ~r1-z;-.toJ.ylt!n aa4Jt~te a,-~6erett to ~~l trt~

.'i'J-~~rwl~iri !:\Ofit&\ts in. e&tf.:Vltif·~. wl'le:v~ :EM; 1}w1betr~'l• .~<l

t~l~ tr~ ... ph~l:iio>ottwll ·~in. tt~~t~'ijf1> w~; c.otrsQ.~l$!'ablll·•~

aottve(t~J.

;.nt~s.~e.atlat:r ·tn·i3G,9tint't~~t. ~;:~orl$l ~ ~el (OOI· ~th t~lk1l nt.ll\ tr.i~IW l tin ~"::l!)Ot~id$ sbow~d . toot in the

!abf)!JGttj;r.y,, tri~tU~:t ~ i'l•~lct.$Pll ··tr1rotylth.ls r..;ce

b$t:tw ttnatot.d~~ tbm1;. tr!~t1n tj:OO:J.O~.o but tbGt th~

reve~e 1e t~u$. in tl~· field., Zhlo ~liUl' be~.n an~1f!od t~

t.~· );.~;~:!" G~btlitJ·· aue ~.~e baeber. ~t1lati.li:ttJ· of tne lf)m•!.':•

~~·~~G'nver~- t~l1J1n cr$Jvoun§1a Q!'e t~e~ o~t()tod:-e t~

'~ia:lJ~ t!t~ Ql)n~o~il!~o ·

::in tfl~ ee:.r.nu 1000'~~ the i?i·v$t (l~~i:lQ'tin OD.'"lt"l>:)OOd•

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