-microscopes- types. goals: 1. check your proficiency 2. develop proficiency 3. proficiencies...
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Knowledge about microscope: A. types B. parts C. care D. use E. theory of operationTRANSCRIPT
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-Microscopes- Types
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Goals:1. Check your proficiency2. Develop proficiency3. Proficiencies include:
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Knowledge about microscope:
A. typesB. partsC. careD. useE. theory of operation
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Proficiency test:
If you pass this test, you have demonstrated proficiency in microscope knowledge.
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Types of microscopesused in Science
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There is a great number of different types of microscopes in use today. We are going to look at just a few which are “common” in use in schools, colleges and universities.
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Dissection Microscope
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Characteristics: low power 3-D (sterioscopic-binocular)$100-$500cannot see cells
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Compound Light Microscope
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Characteristics:Light poweredto 1000x2-D$150-$10,000
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Confocal Microscope
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Characteristics:Laser light poweredto 1000x3-D, digitally enhanced$20,000-$100,000
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Scanning Electron Microscope
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Characteristics:Reflected electrons100,000x3-D imagesubject is gold coated$50k up into the millions
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TransmissionElectronMicroscope
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Characteristics:electrons pass thru specimen200,000x2-D image$50k up into the millions
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The vast majority of other types are used in limited research institutes. Some of these are:
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Scanning probe•AFM: Atomic Force Microscopy•BEEM: Ballistic Electron Emission Microscopy•EFM: Electrostatic Force Microscope •ESTM: Electrochemical Scanning Tunneling Microscope •FMM: Force Modulation Microscopy•KPFM: Kelvin Probe Force Microscopy •MFM: Magnetic Force Microscopy •MRFM: Magnetic Resonance Force Microscopy NSOM: Near-field Scannin Optical Microscopy•PFM: Piezo Force Microscopy •PSTM: Photon Scanning Tunneling Microscopy
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• PTMS: Photothermal Microspectroscopy/Microscopy• SAP: Scanning Atom Probe Microscopy• SCM: Scanning capacitance Microscope• SECM: Scanning ElectroChemical Microscopy• SGM: Scanning Gate Microscopy• SICM: Scanning Ion-Conductance Microscopy• SPSM Spin Polarized Scanning tunneling Microscopy• SThM: Scanning thermal Microscopy• STM: Scanning Tunneling Microscopy• SVM: Scanning Voltage Microscopy• SHPM: Scanning Hall Probe Microscopy• SSM: Scanning SQUID Microscope• RTM: Recurrence Tracking Microscope• SAM: Scanning acoustic microscope
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Microscopes have many technological procedures which improve the results. Some of these include:
•Bright Field Microscopy•Dark Field Microscopy•Digital Microscope•Fluorescence interference contrast Microscopy•Fluorescence Microscopy•Laser Capture Micro dissection
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Brightfield – light beamed from below
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Dark field light hits object leaving back ground black.
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Fluorescence MicroscopyShowing cheek cells stained with fluorescent dye.
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New microscope technology is under constant development, thus the need for life long learning.
Multifocal plane MicroscopyPhase contrast Microscopy
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Tungsten crystal showing atoms at 2,700,000 x
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