M2  15-­‐Oct-­‐12  Berret  

« PROPOSITION DE STAGE ET DE THESE »

Laboratoire : Laboratoire Matière et Systèmes Complexes

Adresse : UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13

Responsable(s) de Stage : Jean-François Berret

Téléphone : 01 57 27 61 47 01 57 27 61 52 Email : jean-francois.berret@univ-paris-diderot.fr

N° et intitulé des écoles Doctorales de rattachement envisagées : ED 518 et ED 107

Titre du stage : “Protein corona” paradigm and nanotoxicity Résumé :   In   the   last   years,   engineered   inorganic   nanoparticles   have   emerged   as   fundamental  constituents   in   the   development   of   nanotechnology.   Concerns   have   been   raised   however   regarding  their  potential  risks  for  human  health  and  for  the  environment.  In  in  vivo  assays  however,  the  effective  unit  of  interest  is  not  the  nanoparticles  itself,  but  the  particle  and  its  “corona”,  which  implies  the  layer  that  forms  around  the  particles,  with  associated  proteins  or  biomolecules  coming  from  the  surrounding  biological  fluid.  In   this   project,   we  will   evaluate   the   validity   of   the   “protein   corona”   paradigm   by   studying   biological  fluids   that   are   close   to   real   life   biofluids   and   determine   the   corresponding   nanoparticle-­‐protein  interactions.  One  fluid  among  all  biological  fluids  is  significantly  relevant  for  human  safety,  the  serum  of  the   blood.   The   serum   is   the   yellowish   liquid   that   includes   the   proteins   (except   those   used   in   blood  clotting)   and   biological   molecules   such   as   antibodies,   antigens,   hormones,   and   any   exogenous  substances.    

The   present   project   aims   to   study   the   “Protein   corona”   in  conditions   that   are   close   to   those   of   real   life   and   natural  environments.  This  will  be  realized  by:  1   -­‐   A   pertinent   choice   of   particles   known   to   impact   human  health   on   production   sites   and   in   polluted   areas.   These  particles   are   nano-­‐oxides   responsible   for   inflammations,  diseases  and  dysfunctions  of  cells.    2   –   The   effect   of   composition   of   the   biofluid   on   particle  corona  will  be  studied,   the  goals  being  the   identification  and  physical  properties  of  the  surrounding  matrix  and  its  influence  on   the   nanoparticles/cell   interactions   and   toxicity.  Experiments   assessing   the   protein   corona   will   be   light  scattering,   electrophoretic   mobility,   scanning   and  transmission  electron  microscopy.  In  vitro  assays  with  protein  coated  particles  will  complete  the  study.      

Recent  References  on  this  work  M.   Safi,   H.   Sarrouj,   O.   Sandre,   N.   Mignet   and   J.-­‐F.   Berret,   Interactions   between   sub-­‐10   nm   iron   and   cerium   oxide  nanoparticles  and  3T3  fibroblasts  :  the  role  of  the  coating  and  aggregation  state,  Nanotechnology  21  (2010)  145103  M.   Safi,  M.   Yan,  M.-­‐A.   Guedeau-­‐Boudeville,   H.   Conjeaud,   V.   Garnier-­‐Thibaud,   N.   Boggetto,   A.   Baeza-­‐Squiban,   Florence  Niedergang,  D.  Averbeck  and   J.-­‐F.  Berret,   Interactions  between  magnetic  nanowires  and   living   cells   :  Uptake,   toxicity  and  degradation,  ACS  Nano  5  (7),  5354-­‐5364  (2011)  M.   Safi,   J.   Courtois,  M.   Seigneuret,   H.   Conjeaud   and   J.-­‐F.   Berret,   The   effects   of   aggregation   and   protein   corona   on   the  cellular  internalization  of  iron  oxide  nanoparticles,  Biomaterials  32  (2011)  9353-­‐9363  

The protein corona that forms around nanoparticles dispersed in biofluids

plays an important role in the evaluation of the nanotoxicity.

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« PROPOSITION DE STAGE ET DE THESE »

Laboratoire : Laboratoire Matière et Systèmes Complexes

Adresse : UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13

Responsable(s) de Stage : Jean-François Berret

Téléphone : 01 57 27 61 47 01 57 27 61 52 Email : jean-francois.berret@univ-paris-diderot.fr

N° et intitulé des écoles Doctorales de rattachement envisagées : ED 518 et ED 107

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Transmission electron microscopy image of 7 nm nanoceria internalized by murine fibroblasts. The vesicles on the right-hand side are endosmomes

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« PROPOSITION DE STAGE ET/OU DE THESE »

Laboratoire : Laboratoire Matière et Systèmes Complexes

Adresse : UMR 7057 Université Paris-Diderot/CNRS, Batiment Condorcet 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13

Responsable(s) de Stage : Jean-François Berret / Gaelle Charron (Itodys, Paris 7)

Téléphone : 01 57 27 61 47 01 57 27 61 52 Email : jean-francois.berret@univ-paris-diderot.fr

N° et intitulé des écoles Doctorales de rattachement envisagées : ED 518 and ED 107: Titre du stage : Micro-rheology of cells Résumé : /0-12134# 56# ,0-#6+5-7+-#18# 8219#:7;#;-81.<:,517#18#<:,,-.=# >,# 56#?:6-;#17#,0-#:@@25+:,517#18#;-81.<:,5176# 1.# 6,.-66-6# ,1# <:+.16+1@5+# 6:<@2-6# ,1# ;-,-.<57-# ,0-5.# A56+165,4=# B0-# .0-12135+:2#@.1@-.,5-6#18#618,#+17;-76-;#<:,,-.C# !"#"#@124<-.6C# 25DE5;#+.46,:26#1.#3-26#:.-#E6E:224#<-:6E.-;#95,0#:#.0-1<-,-.#17#6:<@2-#A12E<-6#18#,0-#1.;-.#18#'#<2=#/0-12134#17#?512135+:2#1?F-+,6#56#<1.-#+0:22-73573#657+-#?512135+:2#6:<@2-6#E6E:224#@.1A5;-#,574#:<1E7,6#18#82E5;=#G5A573#+-226#81.#576,:7+-#0:A-#A12E<-6#18#,0-#1.;-.#18#17-#@5+125,-.#H'I)'"#GJ=#B1#6,E;4#,0-#.0-12135+:2#@.1@-.,5-6#18#6E+0#6:<@2-6C#.-6-:.+0-.6#E6-#-<?-;;-;#+12215;6#9016-#<1,5176C#-5,0-.#@:665A-#1.#:+,5A-C#:+,#:6#@.1?-6#81.#,0-#21+:2#-7A5.17<-7,=#

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Transmission electron microscopy and phase contrast images of magnetic nanowires. These wires are used as embedded rheometers inside living cells