1. laboratory safety, use of the facility and lab equipment training …€¦ · laboratory safety,...
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BSL-3 Core Facility Yong Loo Lin School of Medicine National University of Singapore
FO-005
BSL-3/ABSL-3 TRAINING AND CERTIFICATION PROGRAMME
Name of the trainee
Designation
Dept/Unit
Name of the PI
Tel no.
E-mail address
PART A. Training Checklist The following items shall be explained or demonstrated by the trainer
1. Laboratory safety, use of the facility and lab equipment training
(Module 1)
A minimum of hrs of training has been given in the following procedures:
S/N TOPIC CHECK BOX
COMMENTS/REMARKS
1 Overview of BSL-3 biorisk
management policy,
organization structure and BATA
requirements, WSH Act
including risk assessment and
management (BSL-3 Core
Facility Safety Manual, Chapter
1, 6.1)
2 Roles and responsibilities,
(BSL-3 Core Facility Safety
Manual Chapter 4)
3 BSL-3 health surveillance ,
security clearance, training,
proficiency testing and access
requirements, annual
requirements (BSL-3 Core
Facility Safety Manual, section
2
6.2, 6.3, 7.4.2.4)
4 General BSL-3/ABSL-3 rules
and practices (BSL-3 Core
Facility Safety Manual section
7.1 & 7.2)
5 Good microbiological techniques
including hazard communication
and handling blood and blood
products (BSL-3 Core Facility
Safety Manual section 6.5 and
7.14)
6 Use of laboratory equipment and
consumables (pass-through
cabinet between the corridor and
BSL-3 anteroom, autoclaves,
BSCs, centrifuges, incubators,
freezers, fridge/freezers, vacuum
pump, vortex, heat block,
pipettes)
Specify other equipment:
7 Familiarize with the layout of the
facility including emergency
escape routes.
8 Entry and exit procedures SOP-
CP-001(checking in/out, reading
room ventilation signals,
gowning/degowning PPE, PAPR
usage, use of pass-through
cabinets in changing/degowning
room)
9 Use of special equipment (cell
sorter, cell analyzer , )
Specify other equipment:
10 Facility maintenance
11 Equipment maintenance
Specify equipment:
The items on the above checklist have been explained and/or demonstrated to me.
Date : Trainee’s signature
Date : Trainer’s name and signature
3
2. Waste management and transporting/storing/handling of pathogens and chemicals (Module 2)
A minimum of hrs of training has been given in the following procedures:
S/N TOPIC CHECK BOX
COMMENTS/REMARKS
1 Biowaste management in the
laboratory (BSL-3 Core Facility
Safety Manual section 7.12)
2 Storage of biological agents and
materials within the laboratory
(BSL-3 Core Facility Safety
Manual section 7.9)
3 Importing /exporting and
transporting biological agents
and materials (BSL-3 Core
Facility Safety Manual section
7.6)
4 Handling and storing of
chemicals/radioisotopes
(Chemical and Radioactivity
SOPs)
5 Handling of liquid cultures and
agar plates (BSL-3 Core Facility
Safety Manual 7.14.2 & 7.14.3)
6 Preparation and use of
disinfectants (Chemical SOPs)
7 Handling and disposing sharps
(BSL-3 Core Facility Safety
Manual section 7.12.1.4)
8 Housekeeping (keeping
consumable, cleaning routine,
laundry, refilling supplies) BSL-3
Core Facility Safety Manual
section 7.13
The items on the above checklist have been explained and/or demonstrated to me.
Date : Trainee’s signature
Date : Trainer’s name and signature
4
3. Emergency response training (Module 3)
A minimum of hrs of training has been given in the following procedures:
S/
N TOPIC
CHECK BOX COMMENTS/REMARKS
1 Alarms and responses/conducts
2 Ventilation failure (BSL-3 Core
Facility Safety Manual section
7.15.8)
3 Fire (BSL-3 Core Facility Safety
Manual section 7.15.7)
4 Power failure (BSL-3 Core
Facility Safety Manual section
7.15.9)
5 Conduct during sudden stoppage
of biosafety cabinet (BSL-3 Core
Facility Safety Manual section
7.15.1.7)
6 Conducts during infectious and
non-infectious medical
emergency and personnel
pathogen exposure (BSL-3 Core
Facility Safety Manual section
7.15.1 and 7.15.4)
7 Needles/sharp injury response
(BSL-3 Core Facility Safety
Manual section 7.15.1.4)
8 Burn injury response (BSL-3
Core Facility Safety Manual
section 7.15.2)
9 Spill response conduct (BSL-3
Core Facility Safety Manual
section 7.15.1)
10 Accident/Incident reporting
Anonymous reporting (BSL-3
Core Facility Safety Manual
section 6.7)
11 Location and use of first aid kits,
AED, fire extinguishers, manual
call points, fire blanket, hose reel,
exits (BSL-3 Core Facility Safety
Manual Appendix)
12 Contact information about
Emergency Response Team and
5
its responsibilities (BSL-3 Core
Facility Safety Manual Chapter 2)
14 Security breaches (SOP-ER-004)
The items on the above checklist have been explained and/or demonstrated to me.
Date : Trainee’s signature
Date: Trainer’s name and signature
6
4. Experiment/protocol based training (Module 4)
S/N TOPIC
CHECK BOX
COMMENTS/REMARKS
1 Route of transmission, signs and symptoms of
infection and disease caused by respective
pathogen
2 Preventive measures and post-exposure
evaluation and follow-up plan
3 User-specific experimental protocols
(depending on the type of experiments to be
carried out, e.g. microbiology, virology,
animal experiments)
Date: Trainee’s signature
Date: PI’s name and signature
A minimum of hrs training has been given in the following protocols:
S/N Name of Protocols
Certificatio
n Date
Trainee’s
Signature
Trainer’s
signature
First
Examiner
Signature
Second
Examiner
Signature
1
2
3
4
5
6
7
8
9
10
7
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
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5. Animal related training (Module 5)
A minimum of hrs of training has been given in the following procedures:
S/N TOPIC CHECK BOX
COMMENTS/REMARKS
1 Knowledge of IVC cage system
(SOP-ABSL3-012 and 013)
2 Cage changing (SOP-ABSL-3-
001)
3 Routine rodent husbandry tasks
(SOP-ABSL3-005)
4 Weekend monitoring of animals
(BSL-3 Core Facility Safety
Manual section 7.3.2)
5 Lighting control system and
alarms in the animal rooms
6 Infecting / dissecting animals and
homogenizing tissues
7 Handling of animals in a
biosafety cabinet
8 Handling of needles and syringes
for animal experiments (BSL-3
Core Facility Safety Manual
7.11.1)
9 Disposal of animal carcasses/
wastes (SOP-ABSL3-004)
10 Transporting of infected animal
cages from the experimental
room to autoclaves (SOP-
ABSL3-001)
11 Response to an infected animal
escapes from the biosafety
cabinet and to animal bite/scratch.
(BSL-3 Core Facility Safety
Manual 7.15.1.6)
12 Transporting of autoclaved
infected animal cages from the
BSL-3 to MD2. (SOP-ABSL3-
007)
The protocols on the above checklist have been explained and/or demonstrated to me.
9
Date: Trainee’s signature:
Date: Trainer’s name and signature:
6. Compulsory reading of following relevant policy, SOPs and protocols
S/N Titles CHECK
BOX
Comments/Remarks
1 BSL-3 safety policy (BSL-3 Core
Facility Safety Manual section
1.2)
2 Standard Entry and Exit (SOP-
CP-001)
3 Waste management (BSL-3 Core
Facility Safety Manual section
7.12)
4 Emergency procedures (BSL-3
Core Facility Safety Manual
section 7.15)
5 Reporting of Incident/Accident
(BSL-3 Core Facility Safety
Manual section 6.7)
6 Procurement, storage and
transport of infectious samples
(BSL-3 Core Facility Safety
Manual section 7.6)
7 Operational control (BSL-3 Core
Facility Safety Manual chapter 7)
8 Biosecurity and Noncompliance
(BSL-3 Core Facility Safety
Manual sections 6.4 and 6.8)
9 Risk assessments and
management (BSL-3 Core
10
Facility Safety Manual section
6.1)
I have read the above BSL-3 policy, SOPs, protocols and their risk assessments. I understood contents of these documents. I certify that I will adhere to the procedures as described in the documents.
Date: Trainee’s signature
11
BSL-3 Biosafety Committee use only
All training completed Yes No Date:
Name and signature of supervisor/PI:
Date:
Name and signature of Head of BSL-3 Operations:
Date:
Name and signature of BSL-3 Biosafety Committee Chairperson:
Date:
12
PART B: CERTIFICATION EXAM RESULTS
Module 1: Passed : Yes No
Comments:
Name/ Signature of the first examiner__________________________________
Date:
Name/ Signature of the second examiner__________________________________
Date:
Module 2: Passed : Yes No
Comments:
Name/ Signature of the first examiner__________________________________
Date:
Name/ Signature of the second examiner__________________________________
Date:
Module 3: Passed : Yes No
Comments:
Name/ Signature of the first examiner__________________________________
Date:
Name/ Signature of the second examiner__________________________________
Date:
Module 4: Passed : Yes No
Comments: See Section Module 4 for the protocols certified to work
13
Module 5: Passed : Yes No
Comments:
Name/ Signature of the first examiner__________________________________
Date:
Name/ Signature of the second examiner__________________________________
Date:
14
PART C. ACKNOWLEDGMENT
This will certify that I have been informed of the potential hazards posed by the research to
be conducted in the YLL SoM BSL3 laboratory. I have undergone a period of required training
and an appropriate medical evaluation prior to working in the YLL SoM BSL3 facility. I am
familiar with the safety policy, guidelines and regulation standards and emergency procedures
described in the BSL-3 Core Facility Safety Manual. I will report immediately any known or
suspected exposure to the agent or symptoms of infection with the agent to the biosafety
coordinator and my PI.
Date:
TRAINEE’S SIGNATURE
BSL-3 Facility Operator use only
BSL-3 Access granted Yes No
Date:
Name and signature of Head of BSL-3 Operations:
Date:
Name and signature of BSL-3 Core Facility Director:
Date: