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  Malaria is an infectious disease spread by the female Anopheles mosquito A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research A quick summary of the research

The protozoan P. falciparum has a very complicated lifecycle consisting of three-stages. More introduction stuff maybe,

The lifecycle proteins that are currently being targeted include MSP-1_19, AMA1, and MSP2, all from the blood stage, Pfs48_45 from the sexual stage, and SSP2 from the liver stage. Wow this is complicated is anyone actually reading this. The main focus of this research, though, is to isolate MSP1_19 and insert it into the pGEM-T easy vector as well as a plant expression referred to as the pSAT vector. For future research once all genes have been sub-cloned into the pSAT vector they will then be used to transform carrot calli. The transgenic plants will then co-express the gene products in the carrot plants.

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p - SAT

Steps towards the Edible Malaria Vaccine

Isolation of a surface protein within the

blood stage

MSP-1_19

Isolation of a surface protein within the

sexual stage

Pfs48_45 SSP2

Isolation of a surface protein within the

liver stage

Insert target genes into plant expression vector pSAT.

The pSAT vector can contain up to 5 expression cassettes. Each cassette contains autofluorecsentproteins for identification purposes.

Using Agrobacterium tumefaciens transform carrot calli

Verify gene expression using western blots

Test that the appropriate antigens are being produced

This is where you should put the title of your poster. Clark Kent, Mr. Spock, Hilary Clinton & Dr. Leroy Jenkins

The Name of the Organization can go here. School of …., University of Northern Colorado, Greeley, CO

Introduction Methods Methods

Abstract

Results

References

Figure 1. More methods. More methods More methods More methods More methods More methods More methods More methods More methods More methods

Figure 2. More methods More methods More methods More methods More methods More methods More methods More methods More methods.

Figure 3. More methods More methods More methods More methods More methods More methods More methods More methods More methods More methods

AcknowledgementsWe would like to acknowledge the following for their help in making this

research possible:

• Give props!!

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Conclusion