2 植物病理實驗儀器及無菌操作 - web.nchu.edu.twweb.nchu.edu.tw/~rootdis/plant...
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2
1. . . 484. . .
2.
http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/Sterile.htm
3. http://www.easychip.com.tw/equipment.html
1. autoclave
1211.2 kg / cm
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1
2. Oven180 1-3
2
3. Larminar flow 0.3 m
99.97%
30-60 75%
ethanol 10
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3(Class )
(Class )
pathogen trap filter
(Class )
3
-
4.
1 5PP
PP
0.0001 micron90%
2
(
)
3 < 1 cfu / ml1ppb
18.2Mcm25
4
-
( 5-15M,25) ( 18.2M,25)
5
5.
-
PH
ELISA
-
(PCR) (PCR) Real-Time PCR
Auto Sequncer
-70
6
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1.
(1) 70%
(2) (Larminar flow)
a.
b. c.
d.
10~20
e.
10
300~350 3000~3500
2000
70% 75%
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(3)
2(Sterilization)
mediaContainersInstruments
(endospore)
(virus)
(1) Dry heat
a. Red heat in the flame~
(incubation needle)(incubation loop)
(agar)
b. Flaming after dipping in ethanol~
glass
spreader 75%
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c. Hot air oven~
180
petri-dish
pipettetest tubeflask
mineral oil
40~50
(2) Wet heat
a. Boiling water bath~ 5-10
b. Autoclaving~ 100 121
1.2kg/cm2 10~20
(serum)(antibiotic)(saccharides)
(amino scids)(vitamins)
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(3) Filtration~(filter)
(serum)(antibiotic)(saccharides)
(amino scids)(vitamins)
millipore membrane 0.45mm 0.22mm
7
(4) (Irradiation) ~ -
(Ultraviolet)-
2500~2600A
(5) ~
(denature)
a. -70~75 %
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(beaker)(flask)
b. HgCl2
c. -HCHO
d. -
1.
2. 121 , 15lb, 20
oven
3.
4. pasteur pipet170 , 4
5. 10% hypochloride
121 , 15lb, 20