CoolProductsOrganized. Protected. Standardized.

CoolProducts

CoolBoxTM CoolCellTMCoolRackTM

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Modular tools for cooling, (snap)freezing and

thawing biomedical samples

CoolBoxTM

Ice-free cooling and

freezing

CoolCellTM

Alcohol-free cell

freezing container

CoolRackTM

Modular thermo-

conductive tube and

plate holders

Does this look familiar?

Most researchers still entrust their precious samples to this type of cooling

method

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Here’s the same bucket again….

But in this one, the samples are safely placed in one of our CoolRack modules

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Why are CoolProducts necessary?

� Worldwide effort to develop new standards for

collecting, handling, and processing pre-analytical

samples (blood, tissue, tumor, other biological

specimens or sample materials)� EU: http://www.devicelink.com/ivdt/archive/09/04/003.html

� NCI/NIH: � NCI/NIH: http://biospecimens.cancer.gov/practices/default.asp

� “The lack of standardized, high-quality biospecimens has been widely

recognized as one of the most significant roadblocks to the progress of cancer

research.”

� NIH Office of Biorepositories and Biospecimen Research 2010

Symposium: http://www.brnsymposium.com/meeting/brnsymposium/2010/

� Duke: http://web.genome.duke.edu/cores/biorepository/protocols/

� Molecular Epidemiology Workshop, Shanghai, 2008

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It’s all about the curves….

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….the temperature curves

Each sample goes through the 0oC transition the same way.

Cool, freeze and thaw - identical each and every time

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Consistent curves mean temperature control and experimental

repeatability…..INSTANT STANDARDIZATION

CoolProducts

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CoolBoxTM

Ice-free cooling and

freezing

CoolCellTM

Alcohol-free cell

freezing container

CoolRackTM

Modular thermo-

conductive tube and

plate holders

Versatility- rapidly adapts to any temperature

- can be used for a wide temperature range - from -150oC through +200oC

Consistency & Standardization- uniform temperature profiles for all samples

- inexpensive, comprehensive modular solution-CoolRacks, CoolSinks, CoolBox, CoolTrays

- reduced user error

CoolRack

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Better sample organization

& temperature consistency

- reduced user error

- standardize - assay to assay, user to user, lab to lab, site to site

Organization- indexed & organized sample management

- labels stay dry & legible

- minimize risk of contamination

Patented novel alloy construction

- very rapid thermo-conductivity/adaptivity

Tube holders- some with profile-fit

Plate holders- multi-well & PCR

CoolRack

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- multi-well & PCR

Platforms- rectangular & round

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Modular system is customizable, scalable and versatile

Dry IceLiquid Nitrogen Water Bath

-150oC -78oC 0o to 4oC 37oC +

• Eliminate direct immersion

into LN2

• All tubes are upright

• Same freezing profile for all

• Replaces direct contact with

dry ice

• Eliminates ethanol bath on

the bench – cost savings,

• Gain organization & stability

in ice

• Even temperature distribution

to all samples even as ice melt

• Keeps tubes out of water –

reduced contamination, labels

stay dry

• Consistent thawing profile for

Ice

CoolRack

• Same freezing profile for all

samples

• ThermalTray provides

elevation of samples &

increased distance between

hands and LN2

• Rapidly adapts from ambient

(25oC) to LN2 temp (-150oC) in

12-14 minutes

the bench – cost savings,

reduced hazard & waste

• CoolRack PF provides equal

or faster snap-freezing than

ethanol slurry

• Standardized freezing -

minimized standard deviation in

freeze profiles

• Improved work flow

• Adapts to -78oC in 5-7

minutes

to all samples even as ice melt

• Labels stay dry and legible for

better sample identification

• Reduced risk of sample

contamination

• Rapidly goes from ambient to

<4oC in 90 seconds

•Using ThermalTray, 10-15

hours of <4oC with one pan of

ice

• Consistent thawing profile for

all samples

• All wells at identical

temperature - eliminates “edge

effect” on multi-well plates

• Stable support of tubes &

plates

Temperature control without directly immersing samples

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Cooling - Ice

� Ambient to < 4oC in 60-90 seconds

� 4-6 hours of use when placing CoolRack directly on

ice

� Up to 14 hours cooling with a single pan of ice with

ThermalTray

Cooling rate on ice

CoolRack

Snap-Freezing – Dry Ice

� Equal or better freeze rate than dry

ice/ethanol bath

� No alcohol required – cost savings, reduced

hazard

� Consistent freeze profile for all samples

Freeze rate on dry ice Standard deviation

Cooling rate on ice

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CoolProducts

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CoolBoxTM

Ice-free cooling and

freezing

CoolCellTM

Alcohol-free cell

freezing container

CoolRackTM

Modular thermo-

conductive tube and

plate holders

Ice-free cooling and freezing- 4 hrs w/o lid, 10 hrs with lid (+2oC)

- 3 hrs w/o lid, 6 hrs with lid (-12oC)

Versatile- five temperature options in one box using

cartridges, dry ice or LN2

- portable

- accommodates a variety of tubes & plates

CoolBox

+2oC

Consistent temperature- all wells within 0.1oC regardless of position even

as cartridges thaw

Highly recommended- for highly-sensitive applications such as PCR

and RNA, use in the hood, cleanroom,

vivarium, short transporting, bio-hazardous

containmentPortable ice-free

cooling or freezing

Portable ice-free

cooling or freezing

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CoolBox

-25oC with thermal

regulator pad placed

between CoolRack and

dry ice

Snap-freezing at -78oC

with CoolRack directly on

200cc dry ice

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CoolBox

CoolBox Gel-Filled Container

Temperature range: -150oC through +150oC

Temperature range: Fixed at -20oC or 0oC

Cooling Media:LN2, Dry ice, Wet ice, Cartridge

Cooling Media:Gel

Well-to-well Temperature Variance:0.1oC

Well-to-well Temperature Variance:Up to 4oC

Tube & Plate Formats: Tube & Plate Formats:

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Tube & Plate Formats:0.5ml conical, 1.5ml conical, 1.5-2.0ml cylindrical, 12.5mm dia cryos, mass spec tubes, PCR 96- and 384-well plates, 6-, 12-, 24-, 48-, 96-well plates

Tube & Plate Formats:0.5ml conical, 1.5ml conical, PCR tubes

Cooling Duration:10h (2ºC), 6h (-12ºC), 5h (-25ºC), 6h (-78ºC)

Cooling Duration:8h (0ºC), 3h (-21ºC)

Down Time:None – switch cartridge with another frozen one from the freezer or replenish dry ice, LN2

Down-Time:4-6 hours to re-freeze gel-filled tube rack

CoolBox is more versatile, more consistent

CoolProducts

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CoolBoxTM

Ice-free cooling and

freezing

CoolCellTM

Alcohol-free cell

freezing container

CoolRackTM

Modular thermo-

conductive tube and

plate holders

CoolCell

� Alcohol-free cell freezing� No hassle

� No on-going expense

� No variability

� High reproducibility

� Better cell viability� Better cell viability

� Faster work flow

� CryoPrep System� everything you need for cell prep in the

hood – CoolRack CFT, CoolBox, CoolCell

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It’s all about the curves…..

Fusion time and cooling profiles are highly reproducible from run to run

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Better cell viability

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In primary human cells, CoolCell

significantly outperforms

CoolCell Alcohol-Filled Container

No alcohol •No hazardous fluids•Solid core and insulation design•No pre-cooling required

Requires 250ml alcohol•Replace alcohol every 5 uses (250ml)•Keep track of # of uses•Alcohol degrades slightly each time it’s used•Pre-cool alcohol in refrigerator

Very consistent -1oC/min rate•All vials have uniform freeze rate every time

•Radially symmetric – design enables consistency

Inconsistent freeze rate•Each run is slightly different due to alcohol degradation•Two circles of wells – different freeze rates for each circle

Cost savings ~$338/year maintenance per unit

CoolCell

Cost savings•No maintenance cost•Pays for itself quickly

~$338/year maintenance per unit•Change alcohol weekly (~$6/wk)•Dispose haz waste (~$0.75/wk)•10 units = $3,380 to use EVERY YEAR

Lid opens easily •Not cold to the touch when removing from the -80C freezer

Screw cap very difficult to remove when frozen•Protective glove required when handling frozen unit

Better cell viability Good cell viability

Ready to use again in 5 minutes Takes >1 hour for device to return to room temp for re-use

Low impact on freezer•1/3 the heat impact on freezer compared to alcohol-filled units

Large thermal mass impacts local freezer area•Large heat capacity removed from alcohol impacts nearby samples

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Customer quotes

� Stanford University � “We run a registry, in which large amounts of PBMCs are processed for long-term

cyropreservation. After testing the CoolCell out, we found slightly better cell viability (>90%)

than our current cell freezing containers, and there is no Isopropanol waste generated. Overall,

the CoolCell has proven to us to set a new bar in cryopreservation.”

� Merck Pharmaceuticals � “I have already purchased 21 CoolCells, I have obtained a CoolBox, and I recently ordered a

96well flat bottom CoolBox system. All these will be used for our assays. I need one more 96well flat bottom CoolBox system. All these will be used for our assays. I need one more

product to complete my collection of ice-free cooling devices. I already passed on my evaluation

of the CoolCell and the other groups are pretty excited and are going to use them.“

� UC Berkeley� “Our lab at UC Berkeley recently tried out demo models for the CoolRackM90, CoolSink, and

Thermal Tray and we loved them and thought they were very useful. We were just amazed by

how quickly the Cool Rack chills our tubes within minutes while preventing the possibility of

water getting in and contaminating our samples. ……We decided to get all of them after trying

them out due to their effectiveness. Our lab manager has been looking for a company that was

offering such products and fortunately, we've the right place!”

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Genentech Amgen Pfizer Roche Harvard MITCalTech USCF Glaxo Bristol Myers Squibb Novartis

Translational Genomics Gilead Sciences MerckMedImmune Genpharm Elan Pharmaceuticals

Centocor Boehringer Ingelheim Oncomed NIH/NCITakeda Pharmaceuticals CDC Covance XOMA

Quest Diagnostics Otsuka America PharmaceuticalsSalk Institute Burnham Institute Scripps Institute

Stanford University Yale Genzyme EMD SeronoShire HGT Alnylam Pharmaceuticals Cell-System

Thank you.

Shire HGT Alnylam Pharmaceuticals Cell-SystemJackson Labs Beth Israel Deaconess Hospital HHMI

Whitehead Institute Harvard Systems Biology Exelixis Charles River Labs Sanofi Pasteur Penn StateLawrence Berkeley Lab Batelle UC Berkeley

Cedars Sinai Hospital Cleveland Clinic IDEXX BiogenPerkin Elmer Genetics Blood Systems Research Inst.

BC Cancer Research Center USC Pacific BioscienceUCLA Precision Therapeutics Northwestern

Michelle Nemits

VP Sales & Business Development

Mobile: 415-336-4415

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