2017-18 for setting up microbiology section and

File No. 12015/03/2017-QAFood Safety and Standards Authority of India

(A statutory Authority established under the Food Safety and Standards Act, 2006)(Quality Assurance Division)

FDA Bhawan, Kotla Road, New Delhi – 110002Dated, the 01 Feb, 2018

RFP No. 04/2017-18 FOR SETTING UP MICROBIOLOGY SECTION ANDINSTALLATION OF EQUIPMENTS: CORRIGENDUM

Further to this office Tender Enquiry No. 04/2017-18 dated 03rd January 2018 and Pre-Bid conference held on 12th January 2018.

2. The following amendment are made in the ibid tender:

(a) Last Date and Time for Receipt of Tenders: 26 Feb 2018 at 1500hrs

(b) Date and Time of Opening of Tenders: 26 Feb 2018 at 1530hrs

(c) Part II- Essential Details of items/services required is revised as under:

1. Schedule of Requirements – List of items/services required is as follow :-

Sl.No

Items Qty(Nos)

Purpose

1. Modular clean rooms 01 For Bio-burden reduction in workingarea of microbiology lab.

2. Bio-Safety Cabinet (Class II Type A2) 02 For safe handling of pathogens andsample & For handling media and testitems

3. Vertical Top Loading Autoclave 02 For sterilization of media / glassware

4. Laboratory Refrigerator -2oC – 8oC 02 For Storage of reference cultures andTest reagents / Enzymes etc.

5. Digital Electronic Precision Balance 02 For weighing at low level

6. Circulating water-bath -10°C-100°C, 15 Ltr 02 Tempering of microbial media

7. Incubator (Multi chambered) 01 Suitable for conducting independentincubations in four chambers.

8. Hot Air Oven 01 For sterilization

9. Fumigator / Fogger 01 For room disinfection

Sl.No

Items Qty(Nos)

Purpose

10. Automated pathogen detection andIdentification

01 For rapid detection and identification ofPathogens

11. Automated Microbial enumeration system 01 For rapid enumeration of Pathogens

12. Real Time PCR System 01 Gold Standard for pathogen detection

13. Automatic colony counter (bench-top,digital)

01 For microbial enumeration

14. Anaerobic Chamber 01 For growing anaerobes with strict gasrequirements - Clostridium,Campylobacter, etc.

15. Water purification system 01 For generation of laboratory grade waterfor Microbiological purpose

16. Fully Automated Elisa Reader & Washer 01 For analysis of Staphylococcalendotoxin, Mycotoxins

17. Temperature data logger 06 For routine temperature calibrationchecks

18. Digital Trinocular Microscope with imageprocessing system and digital camera

01 For direct count of microorganisms andtheir structural identification

19. Automatic Safety Bunsen Burner 01 For streaking of pathogens

20. Shaking Incubator 01 For enrichment of bacteria

21. Membrane Filtration System 01 For Water microbiology

22. Stomacher 01 For sample homogenization

23. Air Sampler 01 For routine bio-burden checks of clean-room

24. Laboratory glassware washer/dryer 01 For routine glassware cleaning

25. Bench top UV-visible spectrophotometer 01 For Water Quality Testing

26. Digital Thermohygrometer 01 For Routine monitoring of Room Temp.& Humidity

27. pH Meter 01 For pH checks of prepared Media andSample

Note 1 : The bidders has to quote for all the items mentioned above. In Case bidder fails to quote for allthe items mentioned above his bid will not be considered for evaluation. Consortium is allowed as a singleentity or a subsidiary.

Note 2 : Necessary Civil/Electrical work required for installation of equipment’s mentioned above shall becarried out by the successful bidder.

2. Revised Technical Details:

REVISED SPECIFICATIONS

Sl.No Item Specifications

1. Modular cleanrooms *

Details of Specifications for Cleanroom, Furniture and Lab layout – Attached as ANNEXURE - II

2. Bio-SafetyCabinet(Class II TypeA2)

1. System must work on laminar air flow technology Vertical

2. Working area minimum 4 ft (w) x 2 ft (h) x 2ft Interior work area tobe from a single piece of IS304 grade stainless-steel with largeradius (joint free) corners to simplify cleaning. The cabinet work areamust have s no welded joints, which collect contaminants or rust.

3. External surfaces to be coated with antimicrobial coating to protectagainst surface contamination and inhibit bacterial growth.

4. Work Table: It should be of IS 304 Grade Stainless Steel with finish4 polish surface Front door 5 mm thick Toughened glass, verticalsliding, with Feather touch Motorized operation, while opening thedoor UV Lamp will be cut “OFF” And while closing the door UV Lampwill be “ON” Automatically.

5. Floor standing model with castor wheel and lock

6. System should be class II Type A2 with 70% recirculation and 30%Exhaust using HEPA filter with particle retention better than 99.999%for 0.1- 0.3 micron particles and front accessible for economical andeasy replacement

7. It should have Inbuilt fumigation port for decontamination.

8. Germicidal UV lamp - Controlled by automatic UV lamp timer (lamphours)

a) Emission of 254 nm

b) Lamp should be positioned away from operator line of sight forsafety and proper exposure to interior surfaces.

c) UV lamp should be in working zone (40 micro watts/ square cmat 254 nm or better)

d) The UV lamp should automatically switch “off” when the frontdoor is opened to avoid accidental exposure of UV rays to theusers’.

9. System should have following standard feature a) Nominal inflowvelocity of 95 ±10 feet per minute (fpm) (0.5 m/sec) b) Nominal downflow velocity of 55± 5 fpm (0.3 m/sec)

10. Blower system: It should have one set blower system, which consistsof dynamically & statically balanced aluminium centrifugal impellerdriven by 1/4 HP, single phase,1200- 1400RPM motor, enclosed inan PU coated GI casing suitably suspended in a pair springs &connected to the filter chamber through flexible canvas

11. HEPA filters should have


• Size: 30” x 18” x 3”• Type: Separator less type, Mini-Pleats HEPA Media• Media: Ultra clean glass fiber paper• Retention: 0.3 Micron• Efficiency: 99.997% or better• Initial Pressure: 16 mm WG

• Grade : H13 rating

12. Prefilters:• Size : 600 x 300 x 65 mm• Media : Synthetic, non-woven polyester• Casing : Epoxy painted GI frame• Retention : 10 Micron & above• Efficiency : 90%• Initial Pressure: 6 mm WG

• Grade : F7 rating

13. Interior-mounted, line-of-sight color display Should have LCDinformation centre display showing the following measuredparameters \

• stage velocity,

• total using time,

• UV lamp on/off,

• Differential pressure indicator

• “Filter Life Remaining” bar graph, status line for alarm conditions

• alerts to warn when filter life diminishes to 20%, 10% and 0%

14. Filter monitoring system consisting of an electronically commutatedmotor (ECM) that delivers a precise volume of air as required andautomatically adjusts as filters load without relying on airflowsensors

15. Cabinet should have

1. Pressure gauge,

2. motor voltage regulator,

3. audible and visual window alarm,

4. main and outlet power circuit breakers,

5. Power switches for exterior mounted fluorescent lights and / orultraviolet lights, interior outlets, and blower motor etc

6. Lighting: located outside the contaminated work area.

• High intensity, low wattage >800 lux

• It should be 15 Watts, ,1½ Feet length,- 1 No. each Choke less towithstand larger fluctuations in voltage,

• Must be placed in a position to avoid turbulence in working area


7. Touchpad control on right-hand side post for manual activation ofblower, light, timer, audible alarm mute and menu selection.

8. Radiuses type 304 stainless steel interior and removable, seamless,dished work surface with lift out knobs

9. Service fixture one no with ball-type valve Epoxy-coated steelexterior

10. Towel catch located under work surface

11. Cleaniliness level: The system should have CLASS 100 (ISO 5 forparticle sizes 0.5 µ < 3530 particles/M³ of air at both at Rest &Operation Condition as per ISO 14644 –1

12. Electrical sockets or Pass Through Ports

a) Side mounted switches for minimum three (15/5 amp) electricalsockets for ancillary equipment operation or

b) Convenient rear-wall pass through ports for safe routing ofinstrument cords, cables and leads for 15/5 amps multiple socketwith switches on the wall.

13. System should have RS232 port to transmit the data.

14. Curved stainless-steel inlet grille with Reserve-Air Secondary AirflowSlots or Arm Rest type.

15. Other accessories

•Two gas outlet in the working area, one on each side wall•Leveling Screws & Castor Wheels•DOP test port•Fitted with UV Germicidal lamp for sterilization.•Pre-installed pressure gauge for Measurement of HEPA Filters

Choking system.

16. Alarms: should be Audible or highly visual alarm for

filter replacement warning

installed to indicate loss of exhaust flow. to warn the operator if the window is raised above the

recommended height

17. Certificates required

• Test Certificate for Mini-Pleat HEPA Filters

• Calibration Certificate for Pressure Gauge

• Calibration Certificate for Air Velocity Anemometer,

18. System should come along with the entire necessary accessoryand should be ready to work.

19. For validation vendor should having it own capability with theirown company trained service engineer to perform Cleanlinesslevel validation. No third part validation will be entertained. Onevalidation at the time of installation should be done by companypersonnel.


20. Warranty: Warranty should include parts and labors for 3-years.Warranty should start from 60 days of satisfactory performanceas certified by CFL, Kolkata.

21. Buy-back price for old Biosafety Cabinet – 4 ft [Make: AmarChand & Co., Ambala, India, Year of Installation: 2008] may alsobe quoted

3. Vertical TopLoadingAutoclave - 2nos (Capacity –80 lit, 50 litaprox.)

1. Design - Vertical,

2. Capacity:

80-100 liters internal chamber volume.

50-55 liters internal chamber

3. Single door high pressure steam sterilizer with double/triple walled,steam jacket and separate boiler.

4. Material of construction: Sterilizer chamber SS 304; Door SS 304;Jacket MS; Loading carriage SS 316; Transfer trolley: MS, painted;Door Gasket: Silicon or better; Insulation: fibre glass resin bondedwool or better; Insulation cover: SS sheets.

5. Operating temperature:

• Maximum 123°C

• Temperature Accuracy: ± 0.5 °C at 121 ° C

• Must have Temperature calibration function

6. Operating pressure

• 15 -20 psi

• ANALOG PRESSURE GAUGE (0 -400 psi pressure guage)indicating actual pressure

7. Automatic START/STOP timer

8. Unwrapped Cycle Time Cold:55 Mins, Hot:40 Mins approx.

9. Accelerated cooling technology

10. Sterilizer should be provided with steam generator with Built insteam exhaust bottle.

11. Spring loaded safety valves and automatic vacuum breaker forjacket

12. Removable plug screen for chamber drain.

13. SS baffle for even steam distribution in the chamber.

14. Safety valve protection against poor pressure.

15. Safety lock for door: pressure lock safety device.

16. Advanced Microprocessor based Control Panel

17. Should be equipped with following safety alarms

a) A cycle cannot start if the Automatic START/STOP timer dooris open or not properly locked


b) The door cannot unlock until chamber pressure reaches roompressure

c) Over-Temperature Cut-Off with audio visual alarm

d) Low Temperature Warning: If the temp. stays below 121°C formore than 5 seconds

e) Low Heat Warning: If the temp. does not reach the sterilizationtemperature during the set periods

f) Over-Pressure Cut-Off with audio visual alarm

g) Over Current Cut-off with audio visual alarm.

h) Low Water Level heater cut-off and ALARMS.

18. Should come with inbuilt printer and option to print after every 1minutes during operation and also they should provide externaltemperature sensor.

19. System Configuration Accessories, spares and consumables: a)System as specified b) Should provide available spares andconsumables for at least 10 years c) Should provide a sufficientquality of consumable along with the equipment d) Data logger e)Baskets & containers required for holding flasks, tubes etc. –Number – 2 (1 basket made of punched metal & 1 Bucket withoutholes) Size - 345×181mm (80 lit), 300×182 mm (55 lit) Quality -Stainless Steel

20. Certificate from ISO17025 accredited lab for temperature, pressuregauges & timer.

21. Environmental factors: Shall meet IEC-60601-1-2: 200 (OrEquivalent BIS) General Requirements of Safety forElectromagnetic Compatibility.

22. Power Supply: Power input to be 220-240VAC, 50Hz,/440 V 3Phase as appropriate and fitted with plug compatible with localsockets

23. Warranty: Warranty against all manufacturing defects. Warrantyshould include parts and labors for 3-years. Warranty should startfrom the day of installation as per the convenience and agreementwith CFL, Kolkata.

24. Buy-back price for old Fully Automatic Autoclave – 60 lit[Make: Osworld, Mumbai, India, Year of Installation: 2013] mayalso be quoted

4. LaboratoryRefrigerator -2oC – 8oC (2nos.)

1. Hermetic compressor with Microprocessor Temp. Control (Temp.Range: 2°C to 14°C)

2. Control panel should be at eye level with Digital Temperature display& Alarms

3. Capacity: 300-500 L

4. Fan forced air circulation to ensure stable & uniform preservationenvironment


5. Should be frost free

6. Should have Easy visibility with 2 sliding glass doors with doublepaned glass with heat reflective film to block heat/UV rays

7. Should have Door open Alarm, Hi/Lo Temperature alarm (bothaudible & visual)

8. Should have abnormal Temperature safety device to prevent thecontents of refrigerator from freezing or temperature from risingabnormally while alarms are activated

9. Interiors and exteriors should be chemical resistant and rust free

10. Should have Monitoring hole & Interior fluorescent lamp

11. Shelves should be of rigid wire with polyethylene coating

12. Evaporator: Should be Fin & tube; Forced air circulation type &Condenser: Wire & tube

13. Insulation: CFC Free rigid foamed-in-place polyurethane

14. Warranty period: Minimum TWO years warranty period

15. Operator and service manual essential requirement

16. Quality Certification: Only international quality CE certified product

5. DigitalElectronicPrecision Balance – 2 nos.

1. Type – Top loading Precision Balance

2. Fully automatic time and temperature controlled internal calibrationand balance should be capable to adjust itself Auto zero setting.

3. Range (weight) - 0.01gm - 1200gm

4. Accuracy: 0.01gm

5. Readability: 0.001gm

6. Capacity: 1200gm, Covered type - Glass draft shield with slidingdoor required..

7. Repeatability: 0.001gm

8. Linearity: 0.002gm

9. Response time: 1.5 s

10. Display: Touch Screen

11. Stabilization Time, 2 Seconds (typically).

12. Calibration certificate from NABL accredited calibration laboratoryshould be supplied along with the eqp.

13. Warranty: Minimum 12 months warranty against all manufacturingdefects.

14. Buy-back price for old Precision Balance [Make: Sartorius,LP1200S Year of Installation: 2007] may also be quoted

6. CirculatingWater bath

1. Internal Bath (volume) Capacity - 15 Ltr.

2. Should be rounded, seamless double walled stainless steel bath topreventing rust, chemical damage and contamination. Powdercoating like epoxy coating exterior for easy cleanup


3. Corrosive resistant stainless steel Gabled drip free lid

4. Temperature

a) Temperature range 20°C to 99°C

b) Temperature Accuracy: ± 0.2 °C at 37.0°C

c) Temperature Uniformity: ± 0.5 °C at 37 .0°C

d) Digital LED display for operating status of TEMP

e) Over-Temperature Cut-Off

f) Temperature calibration function

5. Advanced Microprocessor based Control Panel with digital displaywith an accuracy of ±0.5oC

6. Bath consists of two pilot lamp, temperature control knob andON/OFF switch to work on 220/230 volts AC supplied with stirringarrangement without racks and thermometer..

7. Number and types of racks - tube racks for 15ml x 40 and 50ml tubesx 40 – 2 nos each

8. Low level water sensor. Audible warning safety alarms should bethere for high/low temperature warnings, and dry running protection.

9. Instrument should have lift up bath cover; Carrier racks should begiven for flasks and test tubes racks.

10. A cock should be provided to facilitate draining of bath contents.

11. Water bath protective media should be there to preventcontamination and formation of algae.

12. Heating capacity - 2 KW; should have all the accessories requiredfor the functioning of the equipment.

13. All electrical peripherals required for smooth functioning e.g. voltagestabilizer should be provided with the equipment.


7. Incubator(Multichambered) – 2nos

1. Configuration: Multi-chamber: 4 chambered, floor-standing modelwith Castor wheels

2. Capacity (Individual Chamber volume) 50-60 L x 4 chambers

3. Independent Temperature Control of Each Chambers

4. A minimum of 2 nos of SS-304 height adjustable racks in eachchamber.

5. Temperature range (oC): 25-70 oC, ± 0.2 oC accuracy and ±0.5 oCuniformity with programmable Temperature Control with Illumination

6. Temperature and display of each chamber to be controlledindependently).

7. Independent temperature control system for each chamber toprovide precise temperature

8. Stainless Steel 304 Inner Chambers


9. Door specification: Solid installed with lock

10. No. of Perforated shelves per chamber minimum 2 Nos

11. Digital PID Controller or Programmable Controller

12. Over Temperature Protection, Over Current Leakage Breaker

13. Adjustable time and interval

14. Magnetic door closure with positive sealing gasket

15. Suitable on - line UPS (5 KVA) to support the instrument.

16. Certification: Traceable Temperature Calibration certificate for eachchamber from NABL Accredited laboratory with IQ/OQ/PQ validation

17. Each equipment should be supplied with multi channel data loggerfor temperature


19. Buy-back price for old BOD Incubator (2 nos.) [Make: YOMA,YORKO (Double Door) India, Year of Installation: 2009] may alsobe quoted

8. Hot Air Oven 1. External material: 304 Grade Stainless Steel body with powdercoating.

2. Interior material: Fully stainless steel.

3. Inner chamber: Stainless steel structure with adjustable minimum 2shelves.

4. Window: Double layer glass observation window in front side.

5. Type: Bench Top type (Table top model).

6. Temp. Range: Ambient +10°C to +250°C with temperature settingaccuracy ±0.5 °C with forced air circulation for temperatureuniformity

7. Capacity: 200-300L

8. Temperature Accuracy: ±O.5°C

9. Temperature Protection: Automatic over temperature alarm basedprotection system.

10. Timer function: Choice of time (On/Off condition) for automaticsetting.

11. Temp. Control: Microprocessor control with LCD/ LED display.

12. Convection system: Gentle drying and heating with superiortemperature uniformity.

13. Document and Installation: Traceable calibration certificate fromNABL accredited calibration lab. Installation has to be carried by theskilled team with IQ, OQ and PQ documents and on site validationto be carried out to ensure proper working of hot Air Oven. as perspecification.


14. Certification : Traceable Calibration certificate from NABL Accreditedlaboratory with IQ/OQ/PQ validation


16. Buy-back price for old Oven [Make: Heraeus Instrument,Germany, Model T_6 Year of Installation: 2005] may also bequoted

9. Fumigator /Fogger

1. Body should be compact, durable, leak proof and made of stainlesssteel /heavy duty plastic

2. Laboratory fumigator dispenser consistent particle size generationof 5-15 µ, better

3. The blower head should be rust proof inert to Formaldehyde,KMnO4, H2O2 and deliver aerosols uniformly

4. Should be compatible with wide range of disinfectant in a closedroom.

5. Design- With Wheels, Vortex type. Non rotating and non closingnozzle.

6. Provided with Cable should be at least 5 meters in length, ISI marked

7. Tank Capacity- 5 liters. Easy clean, detachable and non corrosivefor chemical

8. The tank capacity, discharge rate and timer on the machine shouldbe so that the disinfectant should be able to disinfect 4000-5000cubic feet in one cycle of 2 hours (max).

9. ELECTRICAL - 200-270V, 50 HZ.


10. Automatedpathogendetection anddetermination

1. System should be a fully automated pathogen screening systemfrom food samples based on the principle of ELFA/ELISA .

2. All protocols for sample testing should be validated as perFDA/AOAC/ AFNOR/ EU/ISO /DIN specifications.

3. All inoculation strips and all reagents required for testing to beprovided.

4. The system should involve only adding of pre enriched sample intoindividual strips containing all other reagents (enzyme conjugate/wash buffer/ substrate).

5. The instrument shall be a multi parametric system and able toperform more than two parameters in the same run.

6. System should be supplied with an accessory for sample heatingdevice.

7. System should be capable of detecting and enumerating:

i) Salmonella species


ii) Listeria species

iii) E.coli

iv) S. aureus enterotoxin

v) Campylobacter

vi) Shigella

vii) Vibrio sps

8. System should be supplied with an accessory system to determineE.coli, Shigella species, Vibrio species, anaerobic bacteria(Clostridium species) from food samples based on colorimetrictechnology.

9. Negative and Positive reference organisms must be supplied withthe kits

10. Detection methods must be available in both kinetic mode and endpoint mode within a day.

11. The results for the Biochemical reactions should be available on anintuitive software which is 21 CFR part 11 compliant with facility ofaudit trail and electronic signature.

12. Biochemical profiling should be done using plastic cardsimpregnated with biochemical substrates specifically for Grampositive cocci, Gram negative cocci, Gram negative rods, Bacillusspecies, Coryneform species, anaerobic bacteria and yeast species.

13. Biochemical profiling should be done by an automatic analyzerallowing automatic filling of test cards with the test suspensionfollowed by automatic internal barcode reading, sealing and loadingof cards in the incubator sections.

14. Analyzer should be connected to a computer with preloadedsoftware capable of kinetic analysis of ongoing reading andproducing results in real time.

15. Software should be capable of creating new organism list in thedatabase apart from the existing database.

16. System should be provided with an accessory system to performautomated Gram staining for positive samples to confirm and furthertesting.

17. System should be provided with a accessory system based on FRETtechnology (Fluorescence Resonance Energy Transfer) coupledwith Melt point peak analysis to detect food borne pathogens.

18. System should be provided with an accessory with specific media todetect anaerobic bacteria from canned food samples / juices usingcolorimetry technology.

19. All test results should be obtained between 24 – 72 hrs.

20. A remote access software should be provided with the system tohelp monitoring of the system remotely and for troubleshooting.


21. System should be accompanied with all accessories like computer,printer, barcode scanner.

22. System should be supported with MS windows operated system andall modular hardware units with sample preparation station, readingstation computer and accessories with barcode scanner USB, colourprinter and provision for integration with LIMS.

23. Software up-gradation should be free of cost for lifetime of system.System should come along with the entire necessary accessory andshould be ready to work. Any accessory system(s) other than thosementioned in the technical specifications, that are required forsatisfactory installation of the system should be quoted and suppliedwith the instrument.

24. The system must have no additional reagent costs. If additionalreagent costs are required please supply details including cost andpreparation time.

25. For validation vender should having it own capability with their owncompany trained service engineer to perform validation. No third partvalidation will be entertained. One validation at the time ofinstallation should be done by company personnel.

26. Suitable UPS system to be provided

27. Warranty: Warranty should include parts and labors for 3-years.Warranty should start from the day of installation as per theconvenience and agreement with CFL, Kolkata.

28. Kits for pathogen screening and identification for 1000 samples maybe quoted

11. AutomatedMicrobialenumerationsystem

1. System should be able to do microbial enumeration from foodsamples using protocols in compliance with AOAC/ AFNOR/ ISOmethods.

2. System should be able to perform automated microbial enumerationin food samples using MPN method in 24 - 48 hrs.

3. System should be able to perform enumeration for the followingparameters with a detection limit up to 4,900,000 CFU/ml or CFU/g:

i) Aerobic count

ii) Total coliforms counts

iii) E.coli counts

iv) Enterobacteriaceae counts

v) S.aureus counts

vi) Lactic acid bacteria counts

vii) Bacillus cereus counts

viii) Yeast & Mould counts.

4. System should be able to do automate sample inoculation.

5. System should be able to do result interpretation automatically.


6. Kits for test provided for testing should contain the culture medium ,containing in a barcoded vial, in dehydrated format and containfluorescent indicator substrate.

7. System should be able to have a throughput of providing test resultsfor 300 - 400 tests in 6 hrs giving results for microbial enumeration.

8. Samples tested on the system should have complete traceabilitywith data integrity for results.

9. System should be supplied with an accessory system for automaticgravimetric dilution of sample preparation along with one pump. Itshould be a self regulating weighing system with drift alarm withaccuracy in compliant with ISO 7218 and ISO6887-1.

10. System should be supplied with an accessory system forhomogenization of sample with flexible speed (slow/normal/fast),blending capacity (80 to 400ml) with adjustable timer (10 secs to3mins) and removable stainless steel paddles, integrated wastedrawer, very low noise level.

11. System should come along with the entire necessary accessory andshould be ready to work. Any accessory system(s) other than thosementioned in the technical specifications, that are required forsatisfactory installation of the system should be quoted and suppliedwith the instrument.

12. The system must have no additional reagent costs. If additionalreagent costs are required please supply details including cost andpreparation time.


14. Suitable UPS system to be supplied


16. Kits for microbial enumeration may be quoted

12. Real Time PCRSystem

The system should be an automated system for both Real Time PCRand post pcr analysis

HARDWARE:

1. The system should be Peltier based PCR machine supporting all ofthe following formats: 96-well plate with optical adhesive cover, 96-well plate with optical flats caps, 8-tubes strips with optical flat caps.

2. The normalization of reaction due to non-PCR related fluctuationsshould be possible by using any passive reference dye is essential.

3. The excitation source should be bright white LED/Laser/halogen andthe detection system should be through photodiode/CCD Camera.

4. The built-in emission filters to support a broader range offluorophores with a higher sensitivity for longer wave length (red


dyes). The system should be configured and calibrated to use anyof the following dyes or a combination thereof: FAM™,SYBR®Green, VIC®, JOE ™, HEX, TET, BY®, NED ™, TAMRA ™, Cy3®,JUN®, ROX™, TEXAS RED®, and capability of multiplexing for fivetargets or better.

5. The hardware must provide Peltier thermal cycling with pre-configured mode for Fast-PCR (40 cycles in less than 35 minutes)as well as Standard-PCR run in the same block.

6. System must have flexibility of running 2-3 different temperaturessimultaneously in the same run with different set of annealingtemperatures in a single run.

7. The system should have temperature range at least 4 oC-100 oC tofacilitate incubation of samples at low temperature.

8. The system should have peak block ramp rate for heating andcooling exceeding 4.6 oC/ second with temperature uniformity of 0.4oC or better and 0.25 Temperature Accuracy. Vendor should specifythe sample ramp rate and should be more than 3.5°C/sec

9. System should support minimum recommended reaction volume of10 µL and thermal cycling conditions to eliminate optimization ofPCR conditions for running the templates from different sourcessimultaneously although lower would be preferred to minimizereagent consumption.

10. The instrument should have real time quantitative PCR installationspecification which demonstrates the ability to distinguish between1.5 fold templates copies with a confidence level equal to 99.5% orbetter to be demonstrated with RNase P instrument verification platerequired to be done at the time of installation.

11. The system should have preferably Touch Screen LCD feature withreal time visuals of amplification plots etc to avoid dependency oncomputer for operation with USB port.

12. Computer: A business line computer (either notebook or tower) forsystem control, operation, analysis, net-working of multiple systemsand a USB port for data export to Power point, Excel or JPEG fileformats with colored laser printer

13. Latest compatible data workstation with all system software andmonitor should be provided with the system.

14. Installation specifications must demonstrate the ability to detectdifferences as small as 1.5 fold or better in target quantities

15. IQ/OQ should be provided for the instrument

16. A compatible 2 KVA true online UPS with 30 minute backup shouldbe provided along with instrument.

17. Vendor should provide a complete line of reagents including 1)TaqMan universal PCR master mix (500 reactions) 2)SYBR Greenmaster mixes (500 reaction) and disposables including tubes, 96well plate for use with the system for onsite application training afterinstallation and 3) TAQMAN RNASE P 96-well instrumentverification plate.

SOFTWARE:


18. Dedicated licensed full version software for primer and probe designmust be included in the supply.

19. The instrument should have licenced software that can analyzemultiple perspectives in the Multiple Plots view, with side by sideviews of all data aspects including the amplification plots, standardcurve, multi-component data plots, and raw data.

20. The system should also include software to support applicationsincluding absolute quantitation, Relative quantitation, multiplex-PCR, allelic discrimination (SNP), high resolution melt curve analysisas well as pathogen detection and plus/minus assay using internalpositive control.

21. The instrument software should have a multi-componentingalgorithm designed to provide precise deconvolution of multiple dyesignals to enable the simultaneous detection of multiplefluorophores,

22. Should be supplied with Software for applications including absolutequantification, relative quantitation /gene expression/ SNP detectionanalysis. Licensed software should also include and supplystatistical analysis tools like Box-Whisker plots to assess Ctdistribution, scatter plots and heat maps to assess samplecorrelation and quality

23. The instrument software should have experimental design wizardand reaction setup information including pipetting protocols.

24. Should support remote monitoring through a web browser-basedsoftware for accessing and analysing data anywhere and anytime inthe worl

25. The vendor should clearly indicate compliance or deviation vis –a visthe tender specifications and should be highlighted in the literatureor manuals.

26. The instrument should be UL approved and manufactured accordingto ISO 9001 standards.

27. The vendor supplying the instrument should have own applicationsupport laboratory in India, preferable in West Bengal for local andefficient after sales service support.

28. Three years warranty with one year spare replacement, if required.

29. Suitable on - line UPS (about 2 KVA) is required to support theinstrument.

13. Automaticcolony counter(bench-top,digital)

1. Camera - CMOS color camera or higher version Digital ZoomMinimum 28X or higher

2. Resolution - Minimum 1 mega pixels or higher

3. Color detection - Optional

4. Counting time - 1000 colonies per second or more

5. Minimum size colony - 0.1 mm or less

6. Lighting - LED and Automatic


7. Counting - Automatic, with manual control

8. Counting on petri dishes 90mm or higher

9. Counting on pour, Surface plates Yes; Optional – Petrifilms,Chromogenics

10. Data export PDF, JPEG, BMP, PNG and EXCEL

11. USB Connection should be there

12. Computer system - Laptop with Windows 10, 3 GB RAM, GraphicsCard, i-5 or higher processor 14 Guarantee 3 years

13. Compliance GLP (Good Laboratory Practice) & full traceability



14. AnaerobicChamber

1. Capacity (Litres) 300-400;

2. Capacity (Petri Dishes) 400 or more

3. Port / Airlock Capacity 30 plates or more via airlock

4. Porthole System Manual or Instant Access Ports

5. Gas Supplies ANO2/N2 with gas regulator, gas leak detector

6. Footswitch Preferably Wireless type

7. Airlock Cycle Time Automatic with timer option

8. Automatic Dehumidifier Fitted as standard

9. Desired purity level: H2O< 1 ppm, O2< 1ppm

10. Piping: Copper or stainless steel

11. Electrical power: 230 V/50-60 Hz, 10 A or 115 V / 50-60 Hz, 20 A or100 V/ 50-60 Hz, 20

12. Glove Ports, Gloves Material: Butyl, thickness 0.4 mm or more

13. Dimensions (w/d/h - mm) 1255 / 720 / 710

14. Weight (lbs/kg) 220 / 100

15. Temperature Range 5°C above ambient up to 45°C

16. Touch screen Control Desirable

17. Circulation Unit: Flow rate of around 20 m3 /h (Working gasNitrogen)

18. Vacuum pump: < 3X10-2 mbar

19. Sliding Tray: Stainless steel or other corrosion free material


15. Ultrapure waterpurificationsystem

1. Ultra pure water system should take at least 100 Micro Siemens ofWater conductivity and should deliver ultra pure product water bypoint of use dispenser with rocker arm, volumetric dispensing andauto shut off facility having

i) Resistivity > 16 Megaohm-cm

ii) Conductivity < 0.06 Micro-Siemens

iii) TOC level < 10 ppb iv) Flow rate > 1 lit / min

2. Should have separate feed water specific purification cartridge andapplication specific polishing cartridge

3. Should have a vertically placed dual wavelength (185 & 254nm)hotcathode, UV lamp with ballast and quartz sleeve placed in aelectro polished housing.

4. Final filter 0.22 micron PVDF validated membrane. System shouldhave option for producing Pyrogen/Rnase-free water with UFcartridge.

5. Point of use gun with rocker arm and volumetric and fixed volumedispensing (3% accuracy), with a green LED

6. Built in coaxial resistivity meter with a cell constant of 0.01/cm and0.1degree C accuracy thermistor

7. Maintenance display for sanitization, exchange purificationcartridges, activation of fast flush, depressurization, air purge

8. Control display showing product water resistivity / conductivity bothcompensated and non compensated mode, product watertemperature, product water resistivity greater or below set point

9. Buy-back price for old Water Purification System [Make:Millipore, U.S.A ELIX 3, 10 AND MILLI Q Year of Installation:2007] may also be quoted

16. FullyAutomatedElisa Reader &Washer

A PC based fully automated ELISA Plate reader with double beam opticswith pre-programmed applications able to support all plate formats Ubottom, V bottom and flat bottom 8/12/96-well micro plates and provisionfor conventional quartz / glass/plastic cuvettes with all the requiredaccessories.

1. Should have inbuilt Shaker with linear/orbital mode

2. Should be automatically programmed with on-board touch screen &soft keys

3. Capable of storing method with analysis:> 100 method programs onthe instrument

4. Detector: Silicon Photodiode dual detector/PMT

a. Wavelength Selection: Wave length selection should be doublemonochromatic with 1nm increment

b. Temperature control: Up to 60 C or better

c. Light Source; Halogen lamp for Visible range


d. Deuterium Lamp for UV range, light source should be autoautomatically selected as per wavelength required.

e. Scan Ordinate Modes: Absorbance, % Transmittance, %Reflectance

f. Resolution: 0.001 A or better.

g. Wavelength Range: 300 –750 nm

h. Accuracy 1% or ± 0.01 A or better for entire range

i. Repeatability: 05 % ± 0.005 A or better

j. Photometric Range: Absorbance 0-3.0 Abs

k. Photometric Accuracy:

I. 1A± 0.015A for single wavelength

II. 2A: ± 0.02A for dual/multiple wavelength

l. Linearity : ± 2 % from 0 to 3.000 A at 405 nm

m. Reproducibility: ± 1 % from 0 to 3.000 A at 405 nm

n. Reading time: < 15 secs for 96 wells

o. Noise: 0.00005 Abs RMS (500nm) or better

p. Stability & Drift: Automatic calibration between each plate reading

q. Baseline flatness: ± 0.0005 Abs or better

5. SoftwareCompatible Software should be user friendly & simple for datahandling with feature like easy to use report publisher, online helpand answer wizard, GLP & audit trail and fully compatible withWindows.System built in features such as real time display of concentration,photometric mode, single /multi-wavelength.System should have capability to do qualitative, quantitative, kineticswith any formulae including validation, transformation, and factorsand floating cut off.,

6. The software should be 21CFR part 11 compliant.7. Validation Plates for hardware validation of absorbance must be

provided

8. Plate Incubator

a. Compact Digitally controlled with orbital shakingb. Should hold two 96 well microplates, for mixing and/or

incubating.c. Speed 400 to 1200 rpm or betterd. Ambient to 40°C with resolution of 0.1°C.e. Digital timer

9. ELISA Microplate Washer:

a. Fully automatic.b. Should Wash flat, round, and V-bottom plates and stripsc. Automatic calibration, alignment, and last row detectiond. Should have 2-4 independent liquid channels

e. Wash volume per well should be programmable

f. Residual aspiration volume < 2μL


g. Auto-water detection of waste and buffers bottle levels.h. With Audible alarm when waste bottle is full and when buffers

are empty

10. ELISA Plates: 96 well ELISA Plates 200 Nos

11. Computer and Printer: Latest configuration factory set branded PCsystem with 22-23’’ Full HD Monitor with printer –B/W – duplex-laser-legal, A4 - 1200dpi-up to 21 ppm –capacity with network card

12. Suitable UPS with 60 mins backup power for washer incubator andreader

13. Certificate from an ISO 17025 accredited calibration lab for spectralcalibration

14. Compliance: IQ/OQ/PQ of instrument and Software should beprovided along with document

15. Operation and training component: The supplier will have to carryout successful Installation at the laboratory premises and provide on– site comprehensive training to scientific personnel operating thesystem till customer satisfaction

16. Warranted for 3 years after satisfactory installation and workingexcluding consumable parts and accessories.

17. Temperaturedata logger

1. Purpose of Equipment: Functions as portable monitor for use inrefrigerators/ Oven/Incubators.

2. It displays and stores data that can be downloaded to a PC with MSwindows supported software.

3. Temperature range – 30˚C to 50˚C

4. Accuracy: 0.3˚C

5. Measuring interval- 1-255 mins

6. Memory Size: 2000 to 2500 Measurements.

7. External Material: Stainless steel/Plastic.

8. Weight: 3 to 5 gm.

9. Power source: internal lithium battery.

10. Battery life available: 5+ years or 1 million measurements.

11. Reading software and cable needs to be provided.

12. The equipment quoted should be CE Certified or USFDA approved.

18. Digital Trinocular Microscopewith imageprocessingsystem anddigital camera

1. Optical system Infinitely corrected system Focus Vertical stagemovement 25mm or more for course stroke vertical stage movement1micron or less for fine stroke Illuminator Lamp house for 100 wattshalogen lamp with DIC upgradable.

2. Revolving nose piece: Reversed sextuple revolving nose pieceshould be upgradable to DIC in future

3. Objectives Plan achromatic 2X N.A 0.06 Plan achromatic 4X N.A0.10 Plane achromatic 10X N.A 0.25 Plane achromatic 40X N.A 0.65(spring) Plane achromatic 100X N.A 1.25 ( spring & oil)


4. Observation field Wide field trinocular eye piece tube with 10X eyepieces of 25mm or more F.O.V 7 Stage Ceramic coated surfacemechanical stage with right hand low drive controlled with left handfor two specimens.

5. Condenser Swing out condenser usable for 2X-100X.

6. Camera & software Digital pool CCD camera approx. 3MP/4MP, with10 bit digitalization, 2048X1500.

7. Software to capture and image processing.

8. Computer system i5 processor, 4GB RAM,500GB HDD, DVR R/ W,TFT 20”. Microscope, camera and software should be from samemanufacturer.

9. Buy Back Price for Leica DM LM/P/11888500 Bright field Microscopewith Image Analyzer, Year of Installation – 2003 may also be quoted

19. AutomaticSafety BunsenBurner

1. Safety Bunsen Burner with flame monitoring and overheatingprotection for safe operation.

2. The flame can be rapidly ignited by a footswitch or the push buttonwithout the need of a lighter or matches.

3. Two adjustment knobs for air and gas to allow easy fine-tuning offlame size and temperature.

4. The Safety Bunsen Burner should be compatible to common gastypes such as butane/propane and natural gas and can beconnected to either a gas distribution system or to different gascartridges.

5. For heating applications or to flame-sterilize necks of largeErlenmeyer flasks, the Safety Bunsen Burner should be equippedwith a long burner head. The quick coupling of the burner head

6. The smooth, chrome-plated metal housing is easy to clean and bothUV- and solvent-resistant.

20. ShakingIncubator

1. Overall internal dimensions (W x D x H): Minimum 62 x 75 x 82 cm

2. Body: Epoxy Powder Coated Steel Chamber made with corrosiveresistant stainless steel

3. Temperature Range: +20°C to 99°C

4. Temperature Accuracy: ± 0.2 °C at 37 .0°C

5. Temperature Uniformity: ± 0.5 °C at 37 .0°C

6. Shaking Motion: Linear (Reciprocal) Motion with interchangeableholders for Erlenmeyer flasks (10ml, 25ml, 50ml, 125ml, 250ml,500ml), test tubes and 1.5-2.0 vials

7. Speed Range: 25 – 400 rpm or better

8. Control: Integrated Microprocessor PID Control Auto STOP

9. Audio and visual alarms for

a. Over-Temperature Cut-Off Alarm (more than 1°C from setpoint)


b. Over RPM cut-off Alarm (more than 5 rpm)

c. Over Current Cut-Off Alarm

d. Completion of programme

10. Digital LED display for operating status of TEMP and RPM

11. Temperature calibration function

12. Programmable controller offering up to 4 modes of timer andparameter control for reduced user intervention.

13. Timer 0.1 to 99.9 hours or continuous mode

14. UV germicidal lights

15. Convenient bath drains

16. Removable bottom plate and shaking insert

17. Clamps and racks 125ml-10nos, 250ml-10nos, 500ml- 10nos. Testtube rack for 20x50ml tube-1 no and test tube rack for 42x15mltubes-1 no. should be quoted.

18. Power requirement: 230V/50-60Hz 16. Suitable Servo VoltageStabilizer should be quoted

19. Warranty should include parts and labors for 3-years. Warrantyshould start from the day of installation as per the convenience andagreement with CFL, Kolkata.

21. Vacuum Pumpfor MembraneFiltrationSystem

1. Number of heads / stages 1 / 1

2. Max. pumping speed at 50/60 Hz 0.7 / 0.85 m3/h

3. Max. pumping speed at 50/60 Hz 0.4 / 0.5 cfm

4. Ultimate vacuum (abs.) 100 / 75 mbar/torr

1. Ambient temperature range (operation) 10 – 40 °C

2. Ambient temperature range (storage) -10 – 60 °C

3. Max. back pressure (abs.) 1.1 bar

4. Inlet connection Hose nozzle DN 8-10 mm

5. Outlet connection Hose nozzle DN 8-10 mm

6. Rated motor power0.04 kW

7. Rated motor speed at 50/60 Hz 1500/1800 min-1

22. SStomacher/LabBlender

1. The unit should have

a. Chamber of stainless steel with an opening door

b. Should have multi-function digital display Provision of adjustableblending power with on screen indicator.

c. Should have provision of removable paddles for cleaning andautoclaving

d. Should have facility for side by side paddle stop.

e. Provision of fully opening door facility for easy cleaning

2. Disposable bag size: Appropriate to the model & capacity quoted


3. Capacity 50-400 ml

4. Temperature Ambient operating temperature 10-35oC.

5. Humidity range Operating relative humidity range should be 10-89%

6. Adjustable timer settings 1sec-60 mins.

7. Paddle speed Variable speed (4-10 strokes /sec or better

8. Sensor To ensures immediate stop of blending in the event of aleakage

9. Accessories Bags (1000 numbers), Bag clips (50 numbers) Bagstorage rack/stand (2 numbers)

10. Bag sealer

11. Operation and training component The supplier will have tocarry out successful Installation at the laboratory premises andprovide on - site comprehensive training for scientific personneloperating the system till customer satisfaction

12. Warranted for 3 years after satisfactory installation and workingexcluding consumable parts and accessories

23. Air Sampler 1. Material - Anodized aluminum

2. Dimensions – Height - 25 cm, Diameter - 11 cm

3. Diameter of Sampling Head - 10 cm

4. Diameter of petri dish: 90 mm (3½ inches)

5. Nominal Airflow - 100 liters / min. + 2.5%

6. Standard Sampling Volumes - 50, 100, 250, 500, 1000 liters

7. Compliance GLP (Good Laboratory Practice) & full traceability



24. Laboratoryglasswarewasher/dryer

1. Chamber volume of Washer/Dryer

Option 1: 150 – 200 liters capacity

Option 2: 200 – 300 liter capacity.

Please quote for both the above options

2. Internal chamber type

Inner chamber, washing arms and tank filters made of high qualityAISI 316 L stainless steel with HEPA filtered chamber

3. Front Glass Door

Glass Door version – Inside chamber must be visible, while inwashing/drying run.


4. Control System

Soft touch LCD display. Microprocessor controlled.

5. Cleaning Liquid Dispenser

Minimum two automatic internal liquid dispenser

6. Standard pre-programmed cycle

a. At least 10 pre-programmed standard cycles. Including Pre-set programs for chemistry glassware, bacteriology (hightemperature), stubborn stains (agar) and volumetricglassware (lower temperature).

b. Additional programs that can be modified to fit any.

c. Water rinses for hot, cold and hot/cold DI water.

d. Self-diagnostic software

e. Electronic security door lock

7. Internal wash temperature control

Fully adjustable wash temp. up to 90deg C

8. External tap water filtering system

Must include all external tap water filtering system, preferably fromlocal supplier

9. Rack systems and accessories to accommodate all types ofglassware (beaker, flask, pipette, petri dish, burette, measuringcylinder, test tube etc) and laboratory items various configurations ofsizes and quantities of jets or baskets to handle all types of glassand lab ware from bottles to pipettes.

10. Racks should be interchangeable between levels.

11. Built in Dryer Unit

Built in forced air dryer unit for drying entire glassware content afterthe wash/rinse cycle.

12. Consumables required for washing/ drying cycle

i) Must provide all necessary washing chemicals for 100 washrun cycle.

ii) All quality washing chemicals must be easily available inIndian market at reasonable price (Indian Rupees). Importedwashing chemicals/ consumables are discouraged.

13. Visual and audible alarms in the event of a malfunction, displayingthe error


a. IQ/OQ compliance

b. Calibration certificates for temperature

15. Installation and Commissioning


The vendor must carry out the installation and commissioning at site,including the installation of tap water filter system. The tap water inletand drain will be provided at site.

16. Warranty Period

Minimum TWO years full comprehensive warranty must be providedfor all parts in this equipment.

17. End User Training at site

Necessary end user training and instructions must be provided to allusers at site.

18. List of present users in India

Must provide the list of users/ customers of this equipment in India.

19. Desirable Specification:

i) Telescopic bearing railing for loading the basket.

ii) Operator and Service manual with all spare parts list.

20. Availability of all spare parts and service support in India.

25. Bench top UV-visiblespectrophotometer

1. System A fully automated spectrophotometer with double beamoptics with pre-programmed applications using conventional quartz/ glass/plastic cuvettes with all the required accessories.

2. Operation keys Instrument should operate immediately afterswitch on with no warming up time

3. Should be automatically programmed with on-board touch screen &soft keys

4. Capable to store method with analysis:> 100 method programs onthe instrument, > 1000 results with data, evaluation results and usedparameters

5. Optical Design Double Beam with sample and reference cuvettepositions; Czerny-Turner Monochromatic/Holographic grating withsealed optics

6. Reference Compartment Should accommodate cells up to 10 mmpath length as standard feature

7. Light Source

a. Halogen lamp for Visible range

b. Deuterium Lamp for UV range, light source should be autoautomatically selected as per wavelength required.

8. Detector Silicon Photodiode dual detector/PMT

9. Scan Ordinate Modes Absorbance, % Transmittance, %Reflectance

10. Resolution 0.1nm or better.

11. Wavelength Range 180 -1100 nm

12. Wavelength Accuracy ± 0.3nm or better for entire range

13. Wavelength Repeatability ± 0.1nm or better


14. Scanning Speed Selectable Variable wavelength scan rate10nm/min to 2500 nm/min or

15. Spectral Bandwidth Variable (0.1/0.2/0.5/1/2/5) nm

16. Photometric Range

a. Absorbance = -4.5 to 4.5 Abs or better.

b. Transmittance & reflectance 0 to 80000 % or better.

17. Photometric Accuracy

a. 0.5 A: ± 0.004A;

b. 1A: ± 0.006A;

c. 2A: ± 0.010A; (440 nm; traceable neutral density filters)

18. Stray Light

a. Max. 0.005% (220 nm NaI) or better,

b. Max. 0.005% (340,370 nm NaNO2) or better

c. Max. 1% (198 nm KCI) or better

19. Noise 0.00005 Abs RMS (500nm) or better

20. Drift < 0.0005 A/hr (500 nm, 1 hour warm-up)

21. Baseline flatness ± 0.0005 Abs or better

22. Application Software Compatible Software should be userfriendly & simple for data handling with feature like easy to use reportpublisher, online help and answer wizard, GLP & audit trail and fullycompatible with Windows.

23. System built in features such as real-time display of concentration,time scan, photometric mode, single/multi-wavelength, capability forevent recording (e.g., addition of reagents)

24. Software should have built in Methods:

a. Absorbance with one or more wavelengths,

b. Scans, Nucleic acids, Proteins, OD 600,

c. Evaluation: via factor, standard and calibration curve

d. Dual wavelength with subtraction and division evaluation

e. Method dependent evaluation:

f. Absorbance, concentration via factor and standard

g. Concentration via standard series using Linear regression,Nonlinear regression with 2nd and 3rd degree polynomials

h. Spline analysis,

i. Linear interpolation (point to point evaluation)

j. Absorbance allocation via subtraction and division

k. Ratio 260/280, 260/230, Molar concentration and total yieldfor nucleic acids.

25. The software should be 21CFR part 11 compliant.


26. Accessories and spares

a. One pair each of 0.5, 1 and 3 ml quartz cuvettes 10 mm pathlength

b. One pair each of 0.5, 1, and 3 ml glass cuvettes 10 mm pathlength

c. Cuvette holder

d. Deuterium Lamp

e. Halogen lamp

f. Holmium oxide glass filters for wavelength calibration.

g. Didymium glass filter to check wavelength accuracy

h. NIST traceable Potassium dichromate

27. Computer and printer Latest configuration factory set brandedPC system with 22-23'' Full HD Monitor with printer -B/W - duplex-laser-legal,A4 - 1200dpi-up to 21 ppm -capacity with network card

28. UPS Suitable UPS with 60 mins backup power

29. Calibration Certificate from an ISO 17025 accredited lab forspectral calibration.

30. Compliance IQ/OQ/PQ of instrument and Software should beprovided along with document

31. Operation and training component The supplier will have tocarry out successful Installation at the laboratory premises andprovide on - site comprehensive training for scientific personneloperating the system till customer satisfaction

32. Warranty Warranted for 3 years after satisfactory installation andworking excluding consumable parts and accessories.

33. Buy-back price for old UV – VIS Spectrophotometer [Make:Varian, Australia CARRY 50 BIO Year of Installation: 1989] mayalso be quoted

26. DigitalTemperatureHumidity Meter

1. Temperature -20 °C to 60 °C ± 0.5 °C - Readability 0.1 °C

2. R.H. 5 % to 95 % R.H. ± 2.5 % - % R.H readability

3. Backlit dual display of humidity and temperature

4. Past record storage capacity

5. Min/Max/Avg data hold

6. Low battery indicator

27. pH cum ORPMeter

1. pH Range -2.000 to 16.000 pH

2. pH Resolution 0.001 pH, 0.01 pH

3. pH Accuracy (@25ºC/77ºF) ±0.01 pH, ±0.002 pH

4. pH Calibration 5 points (Standard mode) 1.68, 4.01 (3.00†), 6.86,7.01, 9.18, 10.01, 12.45, and two custom buffers; 3 points (Basicmode) 4.01; 6.86; 7.01; 9.18; 10.01


5. pH Temperature Compensation ATC: -5.0 to 100.0ºC; 23.0 to212.0°F*

6. mV Range ±1000.0 mV; ±2000.0 mV

7. mV Resolution 0.1 mV

8. mV Accuracy ±0.2 mV (±999.9 mV); ±1 mV (±2000 mV)

9. Relative mV Calibration

10. Single point calibration

11. Temperature Specifications:

a. Temperature Range -20.0 to 120.0 ºC

b. Temperature Resolution 0.1 °C

c. Temperature Accuracy ±0.5 °C

d. °C/°F Yes

12. pH Electrode Diagnostics

Glass and reference junction diagnostics ( HI 11311 & HI 12301only), out of calibration range , probe condition, response time

13. GLP Model

14. Logging: up to 1000 records organized in: Manual log-on-demand(Max. 200 logs), Manual log-on-stability (Max. 200 logs), Intervallogging (Max. 600 samples; 100 lots)

15. Connectivity - 1 micro USB port for charging and PC connectivity, 1USB port for storage

16. Environment - 0 to 50°C (32 to 122°F), RH max 95% non-condensing

17. Battery Type/Life - Built-in rechargeable battery with up to 8 hours ofcontinuous use

18. Power Supply - 5 VDC adapter

19. Dimensions - 202 x 140 x 12.7mm approx

20. Weight 250 g approx.

21. Warranty: 2 years including probe 6 months

6. Revised Eligibility Criteria for Pre-Qualification of bidders:

(a) Average Annual financial turnover, during the last three years should not be lessthan Rs. One Crore. Documentary evidence duly attested by a CharteredAccountant/Company Secretary should be submitted alongwith the Technical Bid.Bidders should also enclose notary attested copy of IT returns filed for the last threefinancial years, notary attested audited copy of audited accounts, balance sheet,annual report etc.

Note : There is no change from para 6(b) to 6(k).

REVISED TECHNICAL BID FORM (B)

The bids of only the technically qualified bidders will be eligible for consideration for opening offinancial bid. The technical bid of the bidders will be evaluated on the basis of specification of theoffered model vis-à-vis the prescribed specification given below :

Part A:

Sl.No Item Specifications Please Specifywhether the

quoted modelmeets the

specification(Yes/No)

Name of theModel and itsSpecification


Details of Specifications forCleanroom, Furniture and Lab layout – Attached as ANNEXURE - II

Part B:






1. System must work on laminar air flowtechnology Vertical

2. Working area minimum 4 ft (w) x 2 ft(h) x 2ft Interior work area to be froma single piece of IS304 gradestainless-steel with large radius (jointfree) corners to simplify cleaning. Thecabinet work area must have s nowelded joints, which collectcontaminants or rust.

3. External surfaces to be coated withantimicrobial coating to protectagainst surface contamination andinhibit bacterial growth.

4. Work Table: It should be of IS 304Grade Stainless Steel with finish 4polish surface Front door 5 mm thickToughened glass, vertical sliding,with Feather touch Motorizedoperation, while opening the door UVLamp will be cut “OFF” And while





closing the door UV Lamp will be“ON” Automatically.

5. Floor standing model with castorwheel and lock

6. System should be class II Type A2with 70% recirculation and 30%Exhaust using HEPA filter withparticle retention better than99.999% for 0.1- 0.3 micron particlesand front accessible for economicaland easy replacement

7. It should have Inbuilt fumigation portfor decontamination.

8. Germicidal UV lamp - Controlled byautomatic UV lamp timer (lamphours)


b) Lamp should be positioned awayfrom operator line of sight forsafety and proper exposure tointerior surfaces.

c) UV lamp should be in workingzone (40 micro watts/ square cmat 254 nm or better)

d) The UV lamp should automaticallyswitch “off” when the front door isopened to avoid accidentalexposure of UV rays to the users’.

9. System should have followingstandard feature a) Nominal inflowvelocity of 95 ±10 feet per minute(fpm) (0.5 m/sec) b) Nominal downflow velocity of 55± 5 fpm (0.3m/sec)

10. Blower system: It should have oneset blower system, which consists ofdynamically & statically balancedaluminium centrifugal impeller drivenby 1/4 HP, single phase,1200-1400RPM motor, enclosed in an PUcoated GI casing suitably suspendedin a pair springs & connected to the





filter chamber through flexiblecanvas


• Size: 30” x 18” x 3”• Type: Separator less type, Mini-Pleats HEPA Media• Media: Ultra clean glass fiberpaper• Retention: 0.3 Micron• Efficiency: 99.997% or better• Initial Pressure: 16 mm WG


12. Prefilters:• Size : 600 x 300 x 65 mm• Media : Synthetic, non-woven

polyester• Casing : Epoxy painted GI frame• Retention : 10 Micron & above• Efficiency : 90%• Initial Pressure: 6 mm WG


13. Interior-mounted, line-of-sight colordisplay Should have LCDinformation centre displayshowing the following measuredparameters \

• stage velocity,


• UV lamp on/off,


• “Filter Life Remaining” bar graph,status line for alarm conditions

• alerts to warn when filter lifediminishes to 20%, 10% and 0%

14. Filter monitoring system consistingof an electronically commutatedmotor (ECM) that delivers a precisevolume of air as required andautomatically adjusts as filters loadwithout relying on airflow sensors






1. Pressure gauge,



4. main and outlet power circuitbreakers,

5. Power switches for exteriormounted fluorescent lights and /or ultraviolet lights, interioroutlets, and blower motor etc

16. Lighting: located outside thecontaminated work area.

• High intensity, low wattage >800lux

• It should be 15 Watts, ,1½ Feetlength,- 1 No. each Choke less towithstand larger fluctuations involtage,

• Must be placed in a position toavoid turbulence in working area

17. Touchpad control on right-hand sidepost for manual activation of blower,light, timer, audible alarm mute andmenu selection.

18. Radiuses type 304 stainless steelinterior and removable, seamless,dished work surface with lift outknobs

19. Service fixture one no with ball-typevalve Epoxy-coated steel exterior

20. Towel catch located under worksurface

21. Cleaniliness level: The system shouldhave CLASS 100 (ISO 5 for particlesizes 0.5 µ < 3530 particles/M³ of airat both at Rest & Operation Conditionas per ISO 14644 –1

22. Electrical sockets or Pass ThroughPorts





a) Side mounted switches forminimum three (15/5 amp)electrical sockets for ancillaryequipment operation or

b) Convenient rear-wall passthrough ports for safe routing ofinstrument cords, cables and leadsfor 15/5 amps multiple socket withswitches on the wall.

23. System should have RS232 port totransmit the data.

24. Curved stainless-steel inlet grille withReserve-Air Secondary Airflow Slotsor Arm Rest type.


•Two gas outlet in the workingarea, one on each side wall

•Leveling Screws & Castor Wheels•DOP test port•Fitted with UV Germicidal lamp for

sterilization.•Pre-installed pressure gauge for

Measurement of HEPA FiltersChoking system.

26. Alarms: should be Audible orhighly visual alarm for


installed to indicate loss ofexhaust flow.

to warn the operator if thewindow is raised above therecommended height


• Test Certificate for Mini-PleatHEPA Filters

• Calibration Certificate forPressure Gauge

• Calibration Certificate for AirVelocity Anemometer,





28. System should come along withthe entire necessary accessoryand should be ready to work.

29. For validation vendor shouldhaving it own capability with theirown company trained serviceengineer to perform Cleanlinesslevel validation. No third partvalidation will be entertained.One validation at the time ofinstallation should be done bycompany personnel.

30. Warranty: Warranty shouldinclude parts and labors for 3-years. Warranty should start from60 days of satisfactoryperformance as certified by CFL,Kolkata.



2. Capacity:

80-100 liters internal chambervolume.


3. Single door high pressure steamsterilizer with double/triple walled,steam jacket and separate boiler.

4. Material of construction: Sterilizerchamber SS 304; Door SS 304;Jacket MS; Loading carriage SS 316;Transfer trolley: MS, painted; DoorGasket: Silicon or better; Insulation:fibre glass resin bonded wool orbetter; Insulation cover: SS sheets.


• Maximum 123°C

• Temperature Accuracy: ± 0.5°C at 121 ° C

• Must have Temperaturecalibration function






• 15 -20 psi

• ANALOG PRESSURE GAUGE(0 -400 psi pressure guage)indicating actual pressure


8. Unwrapped Cycle Time Cold:55Mins, Hot:40 Mins approx.


10. Sterilizer should be provided withsteam generator with Built in steamexhaust bottle.

11. Spring loaded safety valves andautomatic vacuum breaker for jacket

12. Removable plug screen for chamberdrain.

13. SS baffle for even steam distributionin the chamber.

14. Safety valve protection against poorpressure.

15. Safety lock for door: pressure locksafety device.

16. Advanced Microprocessor basedControl Panel

17. Should be equipped with followingsafety alarms

a) A cycle cannot start if theAutomatic START/STOP timerdoor is open or not properly locked

b) The door cannot unlock untilchamber pressure reaches roompressure

c) Over-Temperature Cut-Off withaudio visual alarm

d) Low Temperature Warning: If thetemp. stays below 121°C for morethan 5 seconds

e) Low Heat Warning: If the temp.does not reach the sterilizationtemperature during the set periods





f) Over-Pressure Cut-Off with audiovisual alarm

g) Over Current Cut-off with audiovisual alarm.

h) Low Water Level heater cut-offand ALARMS.

18. Should come with inbuilt printer andoption to print after every 1 minutesduring operation and also theyshould provide external temperaturesensor.

19. System Configuration Accessories,spares and consumables: a) Systemas specified b) Should provideavailable spares and consumablesfor at least 10 years c) Shouldprovide a sufficient quality ofconsumable along with theequipment d) Data logger e) Baskets& containers required for holdingflasks, tubes etc. – Number – 2 (1basket made of punched metal & 1Bucket without holes) Size -345×181mm (80 lit), 300×182 mm(55 lit) Quality - Stainless Steel

20. Certificate from ISO17025 accreditedlab for temperature, pressure gauges& timer.

21. Environmental factors: Shall meetIEC-60601-1-2: 200 (Or EquivalentBIS) General Requirements of Safetyfor Electromagnetic Compatibility.

22. Power Supply: Power input to be220-240VAC, 50Hz,/440 V 3 Phaseas appropriate and fitted with plugcompatible with local sockets

23. Warranty: Warranty against allmanufacturing defects. Warrantyshould include parts and labors for 3-years. Warranty should start from theday of installation as per theconvenience and agreement withCFL, Kolkata.





3. LaboratoryRefrigerator -2oC – 8oC (2nos.)

1. Hermetic compressor withMicroprocessor Temp. Control(Temp. Range: 2°C to 14°C)

2. Control panel should be at eye levelwith Digital Temperature display &Alarms


4. Fan forced air circulation to ensurestable & uniform preservationenvironment


6. Should have Easy visibility with 2sliding glass doors with doublepaned glass with heat reflective filmto block heat/UV rays

7. Should have Door open Alarm, Hi/LoTemperature alarm (both audible &visual)

8. Should have abnormal Temperaturesafety device to prevent the contentsof refrigerator from freezing ortemperature from rising abnormallywhile alarms are activated

9. Interiors and exteriors should bechemical resistant and rust free

10. Should have Monitoring hole &Interior fluorescent lamp

11. Shelves should be of rigid wire withpolyethylene coating

12. Evaporator: Should be Fin & tube;Forced air circulation type &Condenser: Wire & tube

13. Insulation: CFC Free rigid foamed-in-place polyurethane

14. Warranty period: Minimum TWOyears warranty period

15. Operator and service manualessential requirement





16. Quality Certification: Onlyinternational quality CE certifiedproduct

4. DigitalElectronicPrecision Balance

1. Type – Top loading PrecisionBalance

2. Fully automatic time and temperaturecontrolled internal calibration andbalance should be capable to adjustitself Auto zero setting.


4. Accuracy: 0.01gm


6. Capacity: 1200gm, Covered type -Glass draft shield with sliding doorrequired..





11. Stabilization Time, 2 Seconds(typically).

12. Calibration certificate from NABLaccredited calibration laboratoryshould be supplied along with theeqp.

13. Warranty: Minimum 12 monthswarranty against all manufacturingdefects.


1. Internal Bath (volume) Capacity - 15Ltr.

2. Should be rounded, seamlessdouble walled stainless steel bath topreventing rust, chemical damageand contamination. Powder coatinglike epoxy coating exterior for easycleanup

3. Corrosive resistant stainless steelGabled drip free lid

4. Temperature






b) Temperature Accuracy: ± 0.2 °Cat 37.0°C

c) Temperature Uniformity: ± 0.5 °Cat 37 .0°C

d) Digital LED display for operatingstatus of TEMP



5. Advanced Microprocessor basedControl Panel with digital display withan accuracy of ±0.5oC

6. Bath consists of two pilot lamp,temperature control knob andON/OFF switch to work on 220/230volts AC supplied with stirringarrangement without racks andthermometer..

7. Number and types of racks - tuberacks for 15ml x 40 and 50ml tubes x40 – 2 nos each

8. Low level water sensor. Audiblewarning safety alarms should bethere for high/low temperaturewarnings, and dry running protection.

9. Instrument should have lift up bathcover; Carrier racks should be givenfor flasks and test tubes racks.

10. A cock should be provided tofacilitate draining of bath contents.

11. Water bath protective media shouldbe there to prevent contaminationand formation of algae.

12. Heating capacity - 2 KW; shouldhave all the accessories required forthe functioning of the equipment.

13. All electrical peripherals required forsmooth functioning e.g. voltagestabilizer should be provided with theequipment.







1. Configuration: Multi-chamber: 4chambered, floor-standing modelwith Castor wheels

2. Capacity (Individual Chambervolume) 50-60 L x 4 chambers

3. Independent Temperature Control ofEach Chambers

4. A minimum of 2 nos of SS-304 heightadjustable racks in each chamber.

5. Temperature range (oC): 25-70 oC, ±0.2 oC accuracy and ±0.5 oCuniformity with programmableTemperature Control withIllumination

6. Temperature and display of eachchamber to be controlledindependently).

7. Independent temperature controlsystem for each chamber to provideprecise temperature


9. Door specification: Solid installedwith lock

10. No. of Perforated shelves perchamber minimum 2 Nos

11. Digital PID Controller orProgrammable Controller

12. Over Temperature Protection, OverCurrent Leakage Breaker


14. Magnetic door closure with positivesealing gasket

15. Suitable on - line UPS (5 KVA) tosupport the instrument.

16. Certification: Traceable TemperatureCalibration certificate for each





chamber from NABL Accreditedlaboratory with IQ/OQ/PQ validation

17. Each equipment should be suppliedwith multi channel data logger fortemperature


7. Hot Air Oven 1. External material: 304 GradeStainless Steel body with powdercoating.


3. Inner chamber: Stainless steelstructure with adjustable minimum 2shelves.

4. Window: Double layer glassobservation window in front side.

5. Type: Bench Top type (Table topmodel).

6. Temp. Range: Ambient +10°C to+250°C with temperature settingaccuracy ±0.5 °C with forced aircirculation for temperature uniformity



9. Temperature Protection: Automaticover temperature alarm basedprotection system.

10. Timer function: Choice of time(On/Off condition) for automaticsetting.

11. Temp. Control: Microprocessorcontrol with LCD/ LED display.

12. Convection system: Gentle dryingand heating with superiortemperature uniformity.

13. Document and Installation:Traceable calibration certificate fromNABL accredited calibration lab.Installation has to be carried by the





skilled team with IQ, OQ and PQdocuments and on site validation tobe carried out to ensure properworking of hot Air Oven. as perspecification.

14. Certification : Traceable Calibrationcertificate from NABL Accreditedlaboratory with IQ/OQ/PQ validation



1. Body should be compact, durable,leak proof and made of stainlesssteel /heavy duty plastic

2. Laboratory fumigator dispenserconsistent particle size generation of5-15 µ, better

3. The blower head should be rust proofinert to Formaldehyde, KMnO4,H2O2 and deliver aerosols uniformly

4. Should be compatible with widerange of disinfectant in a closedroom.

5. Design- With Wheels, Vortex type.Non rotating and non closing nozzle.

6. Provided with Cable should be atleast 5 meters in length, ISI marked

7. Tank Capacity- 5 liters. Easy clean,detachable and non corrosive forchemical

8. The tank capacity, discharge rateand timer on the machine should beso that the disinfectant should beable to disinfect 4000-5000 cubic feetin one cycle of 2 hours (max).



9. Automatedpathogen

1. System should be a fully automatedpathogen screening system from

detection anddetermination

food samples based on the principleof ELFA/ELISA .

2. All protocols for sample testingshould be validated as perFDA/AOAC/ AFNOR/ EU/ISO /DINspecifications.

3. All inoculation strips and all reagentsrequired for testing to be provided.

4. The system should involve onlyadding of pre enriched sample intoindividual strips containing all otherreagents (enzyme conjugate/ washbuffer/ substrate).

5. The instrument shall be a multiparametric system and able toperform more than two parameters inthe same run.

6. System should be supplied with anaccessory for sample heating device.

7. System should be capable ofdetecting and enumerating:



iii) E.coli


v) Campylobacter

vi) Shigella

vii) Vibrio sps

8. System should be supplied with anaccessory system to determineE.coli, Shigella species, Vibriospecies, anaerobic bacteria(Clostridium species) from foodsamples based on colorimetrictechnology.

9. Negative and Positive referenceorganisms must be supplied with thekits

10. Detection methods must be availablein both kinetic mode and end pointmode within a day.

11. The results for the Biochemicalreactions should be available on anintuitive software which is 21 CFRpart 11 compliant with facility of audittrail and electronic signature.





12. Biochemical profiling should be doneusing plastic cards impregnated withbiochemical substrates specificallyfor Gram positive cocci, Gramnegative cocci, Gram negative rods,Bacillus species, Coryneformspecies, anaerobic bacteria andyeast species.

13. Biochemical profiling should be doneby an automatic analyzer allowingautomatic filling of test cards with thetest suspension followed byautomatic internal barcode reading,sealing and loading of cards in theincubator sections.

14. Analyzer should be connected to acomputer with preloaded softwarecapable of kinetic analysis of ongoingreading and producing results in realtime.

15. Software should be capable ofcreating new organism list in thedatabase apart from the existingdatabase.

16. System should be provided with anaccessory system to performautomated Gram staining for positivesamples to confirm and furthertesting.

17. System should be provided with aaccessory system based on FRETtechnology (FluorescenceResonance Energy Transfer)coupled with Melt point peak analysisto detect food borne pathogens.

18. System should be provided with anaccessory with specific media todetect anaerobic bacteria fromcanned food samples / juices usingcolorimetry technology.

19. All test results should be obtainedbetween 24 – 72 hrs.





20. A remote access software should beprovided with the system to helpmonitoring of the system remotelyand for troubleshooting.

21. System should be accompanied withall accessories like computer, printer,barcode scanner.

22. System should be supported with MSwindows operated system and allmodular hardware units with samplepreparation station, reading stationcomputer and accessories withbarcode scanner USB, colour printerand provision for integration withLIMS.

23. Software up-gradation should be freeof cost for lifetime of system. Systemshould come along with the entirenecessary accessory and should beready to work. Any accessorysystem(s) other than thosementioned in the technicalspecifications, that are required forsatisfactory installation of the systemshould be quoted and supplied withthe instrument.

24. The system must have no additionalreagent costs. If additional reagentcosts are required please supplydetails including cost and preparationtime.

25. For validation vender should havingit own capability with their owncompany trained service engineer toperform validation. No third partvalidation will be entertained. Onevalidation at the time of installationshould be done by companypersonnel.


27. Warranty: Warranty should includeparts and labors for 3-years.Warranty should start from the day of





installation as per the convenienceand agreement with CFL, Kolkata.

28. Kits for pathogen screening andidentification for 1000 samples maybe quoted


1. System should be able to domicrobial enumeration from foodsamples using protocols incompliance with AOAC/ AFNOR/ISO methods.

2. System should be able to performautomated microbial enumeration infood samples using MPN method in24 - 48 hrs.

3. System should be able to performenumeration for the followingparameters with a detection limit upto 4,900,000 CFU/ml or CFU/g:

i) Aerobic count


iii) E.coli counts


v) S.aureus counts




4. System should be able to doautomate sample inoculation.

5. System should be able to do resultinterpretation automatically.

6. Kits for test provided for testingshould contain the culture medium ,containing in a barcoded vial, indehydrated format and containfluorescent indicator substrate.

7. System should be able to have athroughput of providing test resultsfor 300 - 400 tests in 6 hrs givingresults for microbial enumeration.





8. Samples tested on the systemshould have complete traceabilitywith data integrity for results.

9. System should be supplied with anaccessory system for automaticgravimetric dilution of samplepreparation along with one pump. Itshould be a self regulating weighingsystem with drift alarm with accuracyin compliant with ISO 7218 andISO6887-1.

10. System should be supplied with anaccessory system forhomogenization of sample withflexible speed (slow/normal/fast),blending capacity (80 to 400ml) withadjustable timer (10 secs to 3mins)and removable stainless steelpaddles, integrated waste drawer,very low noise level.

11. System should come along with theentire necessary accessory andshould be ready to work. Anyaccessory system(s) other thanthose mentioned in the technicalspecifications, that are required forsatisfactory installation of the systemshould be quoted and supplied withthe instrument.

12. The system must have no additionalreagent costs. If additional reagentcosts are required please supplydetails including cost and preparationtime.







15. Warranty: Warranty should includeparts and labors for 3-years.Warranty should start from the day ofinstallation as per the convenienceand agreement with CFL, Kolkata.

16. Kits for microbial enumeration maybe quoted


The system should be an automatedsystem for both Real Time PCR and postpcr analysis

HARDWARE:

1. The system should be Peltier basedPCR machine supporting all of thefollowing formats: 96-well plate withoptical adhesive cover, 96-well platewith optical flats caps, 8-tubes stripswith optical flat caps.

2. The normalization of reaction due tonon-PCR related fluctuations shouldbe possible by using any passivereference dye is essential.

3. The excitation source should bebright white LED/Laser/halogen andthe detection system should bethrough photodiode/CCD Camera.

4. The built-in emission filters to supporta broader range of fluorophores witha higher sensitivity for longer wavelength (red dyes). The system shouldbe configured and calibrated to useany of the following dyes or acombination thereof: FAM™,SYBR®Green, VIC®, JOE ™, HEX, TET,BY®, NED ™, TAMRA ™, Cy3®,JUN®, ROX™, TEXAS RED®, andcapability of multiplexing for fivetargets or better.

5. The hardware must provide Peltierthermal cycling with pre-configuredmode for Fast-PCR (40 cycles in lessthan 35 minutes) as well asStandard-PCR run in the same block.

6. System must have flexibility ofrunning 2-3 different temperatures





simultaneously in the same run withdifferent set of annealingtemperatures in a single run.

7. The system should have temperaturerange at least 4 oC-100 oC to facilitateincubation of samples at lowtemperature.

8. The system should have peak blockramp rate for heating and coolingexceeding 4.6 oC/ second withtemperature uniformity of 0.4 oC orbetter and 0.25 TemperatureAccuracy. Vendor should specify thesample ramp rate and should bemore than 3.5°C/sec

9. System should support minimumrecommended reaction volume of 10µL and thermal cycling conditions toeliminate optimization of PCRconditions for running the templatesfrom different sourcessimultaneously although lower wouldbe preferred to minimize reagentconsumption.

10. The instrument should have real timequantitative PCR installationspecification which demonstrates theability to distinguish between 1.5 foldtemplates copies with a confidencelevel equal to 99.5% or better to bedemonstrated with RNase Pinstrument verification plate requiredto be done at the time of installation.

11. The system should have preferablyTouch Screen LCD feature with realtime visuals of amplification plots etcto avoid dependency on computer foroperation with USB port.

12. Computer: A business line computer(either notebook or tower) for systemcontrol, operation, analysis, net-working of multiple systems and aUSB port for data export to Powerpoint, Excel or JPEG file formats withcolored laser printer





13. Latest compatible data workstationwith all system software and monitorshould be provided with the system.

14. Installation specifications mustdemonstrate the ability to detectdifferences as small as 1.5 fold orbetter in target quantities

15. IQ/OQ should be provided for theinstrument

16. A compatible 2 KVA true online UPSwith 30 minute backup should beprovided along with instrument.

17. Vendor should provide a completeline of reagents including 1)Taq Manuniversal PCR master mix (500reactions) 2)SYBR Green mastermixes (500 reaction) anddisposables including tubes, 96 wellplate for use with the system foronsite application training afterinstallation and 3) TAQMAN RNASEP 96-well instrument verificationplate.

SOFTWARE:

18. Dedicated licensed full versionsoftware for primer and probe designmust be included in the supply.

19. The instrument should have licencedsoftware that can analyze multipleperspectives in the Multiple Plotsview, with side by side views of alldata aspects including theamplification plots, standard curve,multi-component data plots, and rawdata.

20. The system should also includesoftware to support applicationsincluding absolute quantitation,Relative quantitation, multiplex-PCR,allelic discrimination (SNP), highresolution melt curve analysis as wellas pathogen detection and





plus/minus assay using internalpositive control.

21. The instrument software should havea multi-componenting algorithmdesigned to provide precisedeconvolution of multiple dye signalsto enable the simultaneous detectionof multiple fluorophores,

22. Should be supplied with Software forapplications including absolutequantification, relative quantitation/gene expression/ SNP detectionanalysis. Licensed software shouldalso include and supply statisticalanalysis tools like Box-Whisker plotsto assess Ct distribution, scatter plotsand heat maps to assess samplecorrelation and quality

23. The instrument software should haveexperimental design wizard andreaction setup information includingpipetting protocols.

24. Should support remote monitoringthrough a web browser-basedsoftware for accessing and analysingdata anywhere and anytime in theworl

25. The vendor should clearly indicatecompliance or deviation vis –a vis thetender specifications and should behighlighted in the literature ormanuals.

26. The instrument should be ULapproved and manufacturedaccording to ISO 9001 standards.

27. The vendor supplying the instrumentshould have own application supportlaboratory in India, preferable inWest Bengal for local and efficientafter sales service support.

28. Three years warranty with one yearspare replacement, if required.





29. Suitable on - line UPS (about 2 KVA)is required to support the instrument.


1. Camera - CMOS color camera orhigher version Digital Zoom Minimum28X or higher

2. Resolution - Minimum 1 mega pixelsor higher


4. Counting time - 1000 colonies persecond or more



7. Counting - Automatic, with manualcontrol

8. Counting on petri dishes 90mm orhigher

9. Counting on pour, Surface platesYes; Optional – Petrifilms,Chromogenics

10. Data export PDF, JPEG, BMP, PNGand EXCEL


12. Computer system - Laptop withWindows 10, 3 GB RAM, GraphicsCard, i-5 or higher processor 14Guarantee 3 years

13. Compliance GLP (Good LaboratoryPractice) & full traceability


15. Warranty: Warranty should includeparts and labors for 3-years.Warranty should start from the day of





installation as per the convenienceand agreement with CFL, Kolkata.




3. Port / Airlock Capacity 30 plates ormore via airlock

4. Porthole System Manual or InstantAccess Ports

5. Gas Supplies ANO2/N2 with gasregulator, gas leak detector


7. Airlock Cycle Time Automatic withtimer option

8. Automatic Dehumidifier Fitted asstandard

9. Desired purity level: H2O< 1 ppm,O2< 1ppm


11. Electrical power: 230 V/50-60 Hz, 10A or 115 V / 50-60 Hz, 20 A or 100 V/50-60 Hz, 20

12. Glove Ports, Gloves Material: Butyl,thickness 0.4 mm or more

13. Dimensions (w/d/h - mm) 1255 / 720/ 710

14. Weight (lbs/kg) 220 / 100

15. Temperature Range 5°C aboveambient up to 45°C


17. Circulation Unit: Flow rate of around20 m3 /h (Working gas Nitrogen)


19. Sliding Tray: Stainless steel or othercorrosion free material


1. Ultra pure water system should takeat least 100 Micro Siemens of Waterconductivity and should deliver ultrapure product water by point of usedispenser with rocker arm,





volumetric dispensing and auto shutoff facility having



iii) TOC level < 10 ppb iv) Flowrate > 1 lit / min

2. Should have separate feed waterspecific purification cartridge andapplication specific polishingcartridge

3. Should have a vertically placed dualwavelength (185 & 254nm)hotcathode, UV lamp with ballast andquartz sleeve placed in a electropolished housing.

4. Final filter 0.22 micron PVDFvalidated membrane. System shouldhave option for producingPyrogen/Rnase-free water with UFcartridge.

5. Point of use gun with rocker arm andvolumetric and fixed volumedispensing (3% accuracy), with agreen LED

6. Built in coaxial resistivity meter with acell constant of 0.01/cm and0.1degree C accuracy thermistor

7. Maintenance display for sanitization,exchange purification cartridges,activation of fast flush,depressurization, air purge

8. Control display showing productwater resistivity / conductivity bothcompensated and non compensatedmode, product water temperature,product water resistivity greater orbelow set point

15. FullyAutomated

A PC based fully automated ELISA Platereader with double beam optics with pre-programmed applications able to supportall plate formats U bottom, V bottom and





Elisa Reader &Washer

flat bottom 8/12/96-well micro plates andprovision for conventional quartz /glass/plastic cuvettes with all the requiredaccessories.

1. Should have inbuilt Shaker withlinear/orbital mode

2. Should be automatically programmedwith on-board touch screen & softkeys

3. Capable of storing method withanalysis:> 100 method programs onthe instrument

4. Detector: Silicon Photodiode dualdetector/PMT

a. Wavelength Selection: Wavelength selection should bedouble monochromatic with1nm increment

b. Temperature control: Up to60 C or better

c. Light Source; Halogen lampfor Visible range

d. Deuterium Lamp for UVrange, light source should beauto automatically selectedas per wavelength required.

e. Scan Ordinate Modes:Absorbance, %Transmittance, %Reflectance



h. Accuracy 1% or ± 0.01 A orbetter for entire range

i. Repeatability: 05 % ± 0.005A or better

j. Photometric Range:Absorbance 0-3.0 Abs






III. 1A± 0.015A for singlewavelength

IV. 2A: ± 0.02A fordual/multiple wavelength

l. Linearity : ± 2 % from 0 to3.000 A at 405 nm

m. Reproducibility: ± 1 % from 0to 3.000 A at 405 nm

n. Reading time: < 15 secs for96 wells

o. Noise: 0.00005 Abs RMS(500nm) or better

p. Stability & Drift: Automaticcalibration between eachplate reading

q. Baseline flatness: ± 0.0005Abs or better

5. SoftwareCompatible Software should be userfriendly & simple for data handlingwith feature like easy to use reportpublisher, online help and answerwizard, GLP & audit trail and fullycompatible with Windows.System built in features such as realtime display of concentration,photometric mode, single /multi-wavelength.System should have capability to doqualitative, quantitative, kinetics withany formulae including validation,transformation, and factors andfloating cut off.,

6. The software should be 21CFR part11 compliant.

7. Validation Plates for hardwarevalidation of absorbance must beprovided

8. Plate Incubator

a. Compact Digitally controlledwith orbital shaking

b. Should hold two 96 wellmicroplates, for mixingand/or incubating.





c. Speed 400 to 1200 rpm orbetter

d. Ambient to 40°C withresolution of 0.1°C.

e. Digital timer10. ELISA Microplate Washer:

a. Fully automatic.b. Should Wash flat, round, and V-

bottom plates and stripsc. Automatic calibration, alignment,

and last row detectiond. Should have 2-4 independent

liquid channels

e. Wash volume per well should beprogrammable

f. Residual aspiration volume <2μL

g. Auto-water detection of wasteand buffers bottle levels.

h. With Audible alarm when wastebottle is full and when buffers areempty

11. ELISA Plates: 96 well ELISA Plates200 Nos

12. Computer and Printer: Latestconfiguration factory set branded PCsystem with 22-23’’ Full HD Monitorwith printer –B/W – duplex- laser-legal, A4 - 1200dpi-up to 21 ppm –capacity with network card

13. Suitable UPS with 60 mins backuppower for washer incubator andreader

14. Certificate from an ISO 17025accredited calibration lab for spectralcalibration

15. Compliance: IQ/OQ/PQ ofinstrument and Software should beprovided along with document

16. Operation and training component:The supplier will have to carry outsuccessful Installation at thelaboratory premises and provide on –





site comprehensive training toscientific personnel operating thesystem till customer satisfaction

17. Warranted for 3 years aftersatisfactory installation and workingexcluding consumable parts andaccessories.


1. Purpose of Equipment: Functions asportable monitor for use inrefrigerators/ Oven/Incubators.

2. It displays and stores data that canbe downloaded to a PC with MSwindows supported software.


4. Accuracy: 0.3˚C


6. Memory Size: 2000 to 2500Measurements.

7. External Material: Stainlesssteel/Plastic.


9. Power source: internal lithiumbattery.

10. Battery life available: 5+ years or 1million measurements.

11. Reading software and cable needs tobe provided.

12. The equipment quoted should be CECertified or USFDA approved.

17. Digital Trinocular Microscopewith imageprocessingsystem anddigital camera

1. Optical system Infinitely correctedsystem Focus Vertical stagemovement 25mm or more for coursestroke vertical stage movement1micron or less for fine strokeIlluminator Lamp house for 100 wattshalogen lamp with DIC upgradable.

2. Revolving nose piece: Reversedsextuple revolving nose piece shouldbe upgradable to DIC in future





3. Objectives Plan achromatic 2X N.A0.06 Plan achromatic 4X N.A 0.10Plane achromatic 10X N.A 0.25Plane achromatic 40X N.A 0.65(spring) Plane achromatic 100X N.A1.25 ( spring & oil)

4. Observation field Wide fieldtrinocular eye piece tube with 10Xeye pieces of 25mm or more F.O.V 7Stage Ceramic coated surfacemechanical stage with right hand lowdrive controlled with left hand for twospecimens.

5. Condenser Swing out condenserusable for 2X-100X.

6. Camera & software Digital pool CCDcamera approx. 3MP/4MP, with 10bit digitalization, 2048X1500.

7. Software to capture and imageprocessing.

8. Computer system i5 processor, 4GBRAM,500GB HDD, DVR R/ W, TFT20”. Microscope, camera andsoftware should be from samemanufacturer.


1. Safety Bunsen Burner with flamemonitoring and overheatingprotection for safe operation.

2. The flame can be rapidly ignited by afootswitch or the push button withoutthe need of a lighter or matches.

3. Two adjustment knobs for air andgas to allow easy fine-tuning of flamesize and temperature.

4. The Safety Bunsen Burner should becompatible to common gas typessuch as butane/propane and naturalgas and can be connected to either agas distribution system or to differentgas cartridges.

5. For heating applications or to flame-sterilize necks of large Erlenmeyer





flasks, the Safety Bunsen Burnershould be equipped with a longburner head. The quick coupling ofthe burner head

6. The smooth, chrome-plated metalhousing is easy to clean and bothUV- and solvent-resistant.


1. Overall internal dimensions (W x D xH): Minimum 62 x 75 x 82 cm

2. Body: Epoxy Powder Coated SteelChamber made with corrosiveresistant stainless steel


4. Temperature Accuracy: ± 0.2 °C at37 .0°C

5. Temperature Uniformity: ± 0.5 °C at37 .0°C

6. Shaking Motion: Linear (Reciprocal)Motion with interchangeable holdersfor Erlenmeyer flasks (10ml, 25ml,50ml, 125ml, 250ml, 500ml), testtubes and 1.5-2.0 vials


8. Control: Integrated MicroprocessorPID Control Auto STOP


a. Over-Temperature Cut-OffAlarm (more than 1°C fromset point)

b. Over RPM cut-off Alarm(more than 5 rpm)

c. Over Current Cut-OffAlarm


10. Digital LED display for operatingstatus of TEMP and RPM






12. Programmable controller offering upto 4 modes of timer and parametercontrol for reduced user intervention.

13. Timer 0.1 to 99.9 hours or continuousmode



16. Removable bottom plate and shakinginsert

17. Clamps and racks 125ml-10nos,250ml-10nos, 500ml- 10nos. Testtube rack for 20x50ml tube-1 no andtest tube rack for 42x15ml tubes-1no. should be quoted.

18. Power requirement: 230V/50-60Hz16. Suitable Servo Voltage Stabilizershould be quoted

19. Warranty should include parts andlabors for 3-years. Warranty shouldstart from the day of installation asper the convenience and agreementwith CFL, Kolkata.



2. Max. pumping speed at 50/60 Hz 0.7/ 0.85 m3/h

3. Max. pumping speed at 50/60 Hz 0.4/ 0.5 cfm

4. Ultimate vacuum (abs.) 100 / 75mbar/torr

5. Ambient temperature range(operation) 10 – 40 °C

6. Ambient temperature range(storage) -10 – 60 °C









11. Rated motor speed at 50/60 Hz1500/1800 min-1



a. Chamber of stainless steel withan opening door

b. Should have multi-functiondigital display Provision ofadjustable blending power withon screen indicator.

c. Should have provision ofremovable paddles for cleaningand autoclaving

d. Should have facility for side byside paddle stop.

e. Provision of fully opening doorfacility for easy cleaning

2. Disposable bag size: Appropriate tothe model & capacity quoted


4. Temperature Ambient operatingtemperature 10-35oC.

5. Humidity range Operating relativehumidity range should be 10-89%

6. Adjustable timer settings1sec-60 mins.

7. Paddle speed Variable speed (4-10 strokes /sec or better

8. Sensor To ensures immediatestop of blending in the event of aleakage

9. Accessories Bags (1000numbers), Bag clips (50 numbers)Bag storage rack/stand (2 numbers)

10. Bag sealer

11. Operation and training componentThe supplier will have to carry out

successful Installation at thelaboratory premises and provide on -site comprehensive training for





scientific personnel operating thesystem till customer satisfaction

12. Warranted for 3 years aftersatisfactory installation and workingexcluding consumable parts andaccessories


2. Dimensions – Height - 25 cm,Diameter - 11 cm


4. Diameter of petri dish: 90 mm (3½inches)

5. Nominal Airflow - 100 liters / min. +2.5%

6. Standard Sampling Volumes - 50,100, 250, 500, 1000 liters

7. Compliance GLP (Good LaboratoryPractice) & full traceability


9. Warranty: Warranty should includeparts and labors for 3-years.Warranty should start from the day ofinstallation as per the convenienceand agreement with CFL, Kolkata.





Please quote for both the aboveoptions


Inner chamber, washing arms andtank filters made of high quality AISI





316 L stainless steel with HEPAfiltered chamber

3. Front Glass Door

Glass Door version – Inside chambermust be visible, while inwashing/drying run.

4. Control System

Soft touch LCD display.Microprocessor controlled.


Minimum two automatic internalliquid dispenser


a. At least 10 pre-programmedstandard cycles. IncludingPre-set programs forchemistry glassware,bacteriology (hightemperature), stubborn stains(agar) and volumetricglassware (lowertemperature).

b. Additional programs that canbe modified to fit any.

c. Water rinses for hot, cold andhot/cold DI water.




Fully adjustable wash temp. up to90deg C


Must include all external tap waterfiltering system, preferably from localsupplier

9. Rack systems and accessories toaccommodate all types of glassware(beaker, flask, pipette, petri dish,burette, measuring cylinder, test tubeetc) and laboratory items various





configurations of sizes and quantitiesof jets or baskets to handle all typesof glass and lab ware from bottles topipettes.

10. Racks should be interchangeablebetween levels.


Built in forced air dryer unit for dryingentire glassware content after thewash/rinse cycle.

12. Consumables required for washing/drying cycle

i) Must provide all necessarywashing chemicals for 100wash run cycle.

ii) All quality washing chemicalsmust be easily available inIndian market at reasonableprice (Indian Rupees).Imported washing chemicals/consumables arediscouraged.

13. Visual and audible alarms in theevent of a malfunction, displaying theerror


a. IQ/OQ compliance

b. Calibration certificates fortemperature


The vendor must carry out theinstallation and commissioning atsite, including the installation of tapwater filter system. The tap waterinlet and drain will be provided at site.

16. Warranty Period

Minimum TWO years fullcomprehensive warranty must beprovided for all parts in thisequipment.






Necessary end user training andinstructions must be provided to allusers at site.


Must provide the list of users/customers of this equipment in India.


i) Telescopic bearing railing forloading the basket.

ii) Operator and Service manualwith all spare parts list.

20. Availability of all spare parts andservice support in India


1. System A fully automatedspectrophotometer with doublebeam optics with pre-programmedapplications using conventionalquartz / glass/plastic cuvettes with allthe required accessories.

2. Operation keys Instrument shouldoperate immediately after switch onwith no warming up time

3. Should be automaticallyprogrammed with on-board touchscreen & soft keys

4. Capable to store method withanalysis:> 100 method programs onthe instrument, > 1000 results withdata, evaluation results and usedparameters

5. Optical Design Double Beam withsample and reference cuvettepositions; Czerny-TurnerMonochromatic/Holographic gratingwith sealed optics

6. Reference Compartment Shouldaccommodate cells up to 10 mm pathlength as standard feature

7. Light Source





a. Halogen lamp for Visiblerange

b. Deuterium Lamp for UVrange, light source shouldbe auto automaticallyselected as perwavelength required.

8. Detector Silicon Photodiode dualdetector/PMT

9. Scan Ordinate ModesAbsorbance, % Transmittance, %

Reflectance


11. Wavelength Range 180 -1100nm

12. Wavelength Accuracy ± 0.3nm orbetter for entire range

13. Wavelength Repeatability ±0.1nm or better

14. Scanning Speed SelectableVariable wavelength scan rate10nm/min to 2500 nm/min or

15. Spectral Bandwidth Variable(0.1/0.2/0.5/1/2/5) nm


a. Absorbance = -4.5 to 4.5Abs or better.

b. Transmittance &reflectance 0 to 80000 %or better.


a. 0.5 A: ± 0.004A;

b. 1A: ± 0.006A;

c. 2A: ± 0.010A; (440 nm;traceable neutral densityfilters)

18. Stray Light

a. Max. 0.005% (220 nm NaI)or better,





b. Max. 0.005% (340,370 nmNaNO2) or better

c. Max. 1% (198 nm KCI) orbetter

19. Noise 0.00005 Abs RMS(500nm) or better

20. Drift < 0.0005 A/hr (500 nm, 1hour warm-up)

21. Baseline flatness ± 0.0005 Abs orbetter

22. Application Software CompatibleSoftware should be user friendly &simple for data handling with featurelike easy to use report publisher,online help and answer wizard, GLP& audit trail and fully compatible withWindows.

23. System built in features such as real-time display of concentration, timescan, photometric mode,single/multi-wavelength, capabilityfor event recording (e.g., addition ofreagents)

24. Software should have built inMethods:

a. Absorbance with one ormore wavelengths,

b. Scans, Nucleic acids,Proteins, OD 600,

c. Evaluation: via factor,standard and calibrationcurve

d. Dual wavelength withsubtraction and divisionevaluation

e. Method dependentevaluation:

f. Absorbance,concentration via factorand standard





g. Concentration viastandard series usingLinear regression,Nonlinear regression with2nd and 3rd degreepolynomials

h. Spline analysis,

i. Linear interpolation (pointto point evaluation)

j. Absorbance allocation viasubtraction and division

k. Ratio 260/280, 260/230,Molar concentration andtotal yield for nucleic acids.

25. The software should be 21CFR part11 compliant.


a. One pair each of 0.5, 1 and3 ml quartz cuvettes 10mm path length

b. One pair each of 0.5, 1,and 3 ml glass cuvettes 10mm path length

c. Cuvette holder

d. Deuterium Lamp

e. Halogen lamp

f. Holmium oxide glass filtersfor wavelength calibration.

g. Didymium glass filter tocheck wavelengthaccuracy

h. NIST traceable Potassiumdichromate

27. Computer and printer Latestconfiguration factory set branded PCsystem with 22-23'' Full HD Monitorwith printer -B/W - duplex- laser-legal,A4 - 1200dpi-up to 21 ppm -capacity with network card





28. UPS Suitable UPS with 60 minsbackup power

29. Calibration Certificate from anISO 17025 accredited lab for spectralcalibration.

30. Compliance IQ/OQ/PQ ofinstrument and Software should beprovided along with document

31. Operation and training componentThe supplier will have to carry out

successful Installation at thelaboratory premises and provide on -site comprehensive training forscientific personnel operating thesystem till customer satisfaction

32. Warranty Warranted for 3 yearsafter satisfactory installation andworking excluding consumable partsand accessories.


1. Temperature -20 °C to 60 °C ± 0.5 °C- Readability 0.1 °C

2. R.H. 5 % to 95 % R.H. ± 2.5 % - %R.H readability

3. Backlit dual display of humidity andtemperature




26. pH cum ORPMeter

1. pH Range -2.000 to 16.000 pH


3. pH Accuracy (@25ºC/77ºF) ±0.01pH, ±0.002 pH

4. pH Calibration 5 points (Standardmode) 1.68, 4.01 (3.00†), 6.86,7.01, 9.18, 10.01, 12.45, and twocustom buffers; 3 points (Basicmode) 4.01; 6.86; 7.01; 9.18; 10.01





5. pH Temperature CompensationATC: -5.0 to 100.0ºC; 23.0 to212.0°F*

6. mV Range ±1000.0 mV; ±2000.0 mV


8. mV Accuracy ±0.2 mV (±999.9 mV);±1 mV (±2000 mV)




a. Temperature Range -20.0 to120.0 ºC



d. °C/°F Yes


Glass and reference junctiondiagnostics ( HI 11311 & HI 12301only), out of calibration range , probecondition, response time

13. GLP Model

14. Logging: up to 1000 recordsorganized in: Manual log-on-demand(Max. 200 logs), Manual log-on-stability (Max. 200 logs), Intervallogging (Max. 600 samples; 100 lots)

15. Connectivity - 1 micro USB port forcharging and PC connectivity, 1 USBport for storage

16. Environment - 0 to 50°C (32 to122°F), RH max 95% non-condensing

17. Battery Type/Life - Built-inrechargeable battery with up to 8hours of continuous use


19. Dimensions - 202 x 140 x 12.7mmapprox






21. Warranty: 2 years including probe 6months

Note : List of Installations of the quoted Model or a comparable model of equivalent sensitivitypreferably in food analysis sector in India (Attach Performance certificate from the organizationswhere the quoted model or a comparable model of equivalent sensitivity has already beeninstalled)

(d) Part IV- Special Conditions of RFP is revised as under:

4. Payment Terms: The payment will be made as per the following terms onproduction of the requisite documents:

S.N. Amount to be paid,INR

Condition(s) for release

Part A1. 100% of the total cost

of setting up ofmodular clean roomand furniture

On Completion of civil/electrical work andreceipt of furniture

Part B1 80 % of the cost of

equipmentOn satisfactory installation andcommissioning of the equipments

2 Balance 20% of thecost of equipment

On successful demonstration of the facility,training and validation

Note : LC may be opened on request for equipments.

14 (b) Response time: The response time of the Seller should not exceed 72hours from the time the breakdown intimation is provided by the Buyer.

(e) Part V- Evaluation Criteria and Price Bid issue is revised as under:

2. Revised Price Bid Format :The Revised Price Bid Format is given below and Biddersare required to fill this up correctly with full details, as required under Part-II of RFP :-

(a) Basic cost of the item/items:

Cost Details

Part A: Basic Cost of Setting up :

Sl.No Item Specifications Cost in INR


Details of Specifications for Cleanroom,Furniture and Lab lay out – Attached asANNEXURE - II

Total Cost of (A)

Part B: Basic Cost of item/items :



1. System must work on laminar air flow technologyVertical

2. Working area minimum 4 ft (w) x 2 ft (h) x 2ftInterior work area to be from a single piece ofIS304 grade stainless-steel with large radius(joint free) corners to simplify cleaning. Thecabinet work area must have s no welded joints,which collect contaminants or rust.

3. External surfaces to be coated with antimicrobialcoating to protect against surface contaminationand inhibit bacterial growth.

4. Work Table: It should be of IS 304 GradeStainless Steel with finish 4 polish surface Frontdoor 5 mm thick Toughened glass, verticalsliding, with Feather touch Motorized operation,while opening the door UV Lamp will be cut “OFF”And while closing the door UV Lamp will be “ON”Automatically.

5. Floor standing model with castor wheel and lock

6. System should be class II Type A2 with 70%recirculation and 30% Exhaust using HEPA filterwith particle retention better than 99.999% for0.1- 0.3 micron particles and front accessible foreconomical and easy replacement


7. It should have Inbuilt fumigation port fordecontamination.

8. Germicidal UV lamp - Controlled by automatic UVlamp timer (lamp hours)


b) Lamp should be positioned away fromoperator line of sight for safety and properexposure to interior surfaces.

c) UV lamp should be in working zone (40 microwatts/ square cm at 254 nm or better)

d) The UV lamp should automatically switch “off”when the front door is opened to avoidaccidental exposure of UV rays to the users’.

9. System should have following standard featurea) Nominal inflow velocity of 95 ±10 feet perminute (fpm) (0.5 m/sec) b) Nominal down flowvelocity of 55± 5 fpm (0.3 m/sec)

10. Blower system: It should have one set blowersystem, which consists of dynamically &statically balanced aluminium centrifugalimpeller driven by 1/4 HP, single phase,1200-1400RPM motor, enclosed in an PU coated GIcasing suitably suspended in a pair springs &connected to the filter chamber through flexiblecanvas


• Size: 30” x 18” x 3”• Type: Separator less type, Mini-Pleats HEPAMedia• Media: Ultra clean glass fiber paper• Retention: 0.3 Micron• Efficiency: 99.997% or better• Initial Pressure: 16 mm WG


12. Prefilters:• Size : 600 x 300 x 65 mm• Media : Synthetic, non-woven polyester• Casing : Epoxy painted GI frame• Retention : 10 Micron & above• Efficiency : 90%• Initial Pressure: 6 mm WG


13. Interior-mounted, line-of-sight color displayShould have LCD information centre displayshowing the following measured parameters \


• stage velocity,


• UV lamp on/off,


• “Filter Life Remaining” bar graph, status linefor alarm conditions

• alerts to warn when filter life diminishes to20%, 10% and 0%

14. Filter monitoring system consisting of anelectronically commutated motor (ECM) thatdelivers a precise volume of air as required andautomatically adjusts as filters load withoutrelying on airflow sensors


1. Pressure gauge,



4. main and outlet power circuit breakers,

5. Power switches for exterior mountedfluorescent lights and / or ultraviolet lights,interior outlets, and blower motor etc.

16. Lighting: located outside the contaminated workarea.

• High intensity, low wattage >800 lux

• It should be 15 Watts, ,1½ Feet length,- 1 No.each Choke less to withstand larger fluctuationsin voltage,

• Must be placed in a position to avoidturbulence in working area

17. Touchpad control on right-hand side post formanual activation of blower, light, timer, audiblealarm mute and menu selection.

18. Radiuses type 304 stainless steel interior andremovable, seamless, dished work surface withlift out knobs

19. Service fixture one no with ball-type valveEpoxy-coated steel exterior

20. Towel catch located under work surface

21. Cleaniliness level: The system should haveCLASS 100 (ISO 5 for particle sizes 0.5 µ < 3530particles/M³ of air at both at Rest & OperationCondition as per ISO 14644 –1


22. Electrical sockets or Pass Through Ports

a) Side mounted switches for minimum three(15/5 amp) electrical sockets for ancillaryequipment operation or

b) Convenient rear-wall pass through ports forsafe routing of instrument cords, cables andleads for 15/5 amps multiple socket with switcheson the wall.

23. System should have RS232 port to transmit thedata.

24. Curved stainless-steel inlet grille with Reserve-Air Secondary Airflow Slots or Arm Rest type.


•Two gas outlet in the working area, one oneach side wall

•Leveling Screws & Castor Wheels•DOP test port•Fitted with UV Germicidal lamp for sterilization.•Pre-installed pressure gauge for

Measurement of HEPA Filters Choking system.

26. Alarms: should be Audible or highly visualalarm for


installed to indicate loss of exhaust flow. to warn the operator if the window is raised

above the recommended height


• Test Certificate for Mini-Pleat HEPA Filters

• Calibration Certificate for Pressure Gauge

• Calibration Certificate for Air VelocityAnemometer,

28. System should come along with the entirenecessary accessory and should be ready towork.

29. For validation vendor should having it owncapability with their own company trainedservice engineer to perform Cleanliness levelvalidation. No third part validation will beentertained. One validation at the time ofinstallation should be done by companypersonnel.

31. Warranty: Warranty should include parts andlabors for 3-years. Warranty should start from


60 days of satisfactory performance ascertified by CFL, Kolkata.



2. Capacity:

80-100 liters internal chamber volume.


3. Single door high pressure steam sterilizer withdouble/triple walled, steam jacket and separateboiler.

4. Material of construction: Sterilizer chamber SS304; Door SS 304; Jacket MS; Loading carriageSS 316; Transfer trolley: MS, painted; DoorGasket: Silicon or better; Insulation: fibre glassresin bonded wool or better; Insulation cover: SSsheets.


• Maximum 123°C

• Temperature Accuracy: ± 0.5 °C at 121 °C

• Must have Temperature calibrationfunction


• 15 -20 psi

• ANALOG PRESSURE GAUGE (0 -400 psipressure guage) indicating actual pressure


8. Unwrapped Cycle Time Cold:55 Mins, Hot:40Mins approx.


10. Sterilizer should be provided with steamgenerator with Built in steam exhaust bottle.

11. Spring loaded safety valves and automaticvacuum breaker for jacket

12. Removable plug screen for chamber drain.

13. SS baffle for even steam distribution in thechamber.

14. Safety valve protection against poor pressure.

15. Safety lock for door: pressure lock safety device.

16. Advanced Microprocessor based Control Panel

17. Should be equipped with following safety alarms


a) A cycle cannot start if the AutomaticSTART/STOP timer door is open or notproperly locked

b) The door cannot unlock until chamberpressure reaches room pressure

c) Over-Temperature Cut-Off with audio visualalarm

d) Low Temperature Warning: If the temp. staysbelow 121°C for more than 5 seconds

e) Low Heat Warning: If the temp. does not reachthe sterilization temperature during the setperiods

f) Over-Pressure Cut-Off with audio visual alarm

g) Over Current Cut-off with audio visual alarm.

h) Low Water Level heater cut-off and ALARMS.

18. Should come with inbuilt printer and option toprint after every 1 minutes during operation andalso they should provide external temperaturesensor.

19. System Configuration Accessories, spares andconsumables: a) System as specified b) Shouldprovide available spares and consumables for atleast 10 years c) Should provide a sufficientquality of consumable along with the equipmentd) Data logger e) Baskets & containers requiredfor holding flasks, tubes etc. – Number – 2 (1basket made of punched metal & 1 Bucketwithout holes) Size - 345×181mm (80 lit),300×182 mm (55 lit) Quality - Stainless Steel

20. Certificate from ISO17025 accredited lab fortemperature, pressure gauges & timer.

21. Environmental factors: Shall meet IEC-60601-1-2: 200 (Or Equivalent BIS) GeneralRequirements of Safety for ElectromagneticCompatibility.

22. Power Supply: Power input to be 220-240VAC,50Hz,/440 V 3 Phase as appropriate and fittedwith plug compatible with local sockets

23. Warranty: Warranty against all manufacturingdefects. Warranty should include parts andlabors for 3-years. Warranty should start from theday of installation as per the convenience andagreement with CFL, Kolkata.

3. LaboratoryRefrigerator -

1. Hermetic compressor with MicroprocessorTemp. Control (Temp. Range: 2°C to 14°C)


2oC – 8oC (2nos.)

2. Control panel should be at eye level with DigitalTemperature display & Alarms


4. Fan forced air circulation to ensure stable &uniform preservation environment


6. Should have Easy visibility with 2 sliding glassdoors with double paned glass with heatreflective film to block heat/UV rays

7. Should have Door open Alarm, Hi/LoTemperature alarm (both audible & visual)

8. Should have abnormal Temperature safetydevice to prevent the contents of refrigeratorfrom freezing or temperature from risingabnormally while alarms are activated

9. Interiors and exteriors should be chemicalresistant and rust free

10. Should have Monitoring hole & Interiorfluorescent lamp

11. Shelves should be of rigid wire with polyethylenecoating

12. Evaporator: Should be Fin & tube; Forced aircirculation type & Condenser: Wire & tube

13. Insulation: CFC Free rigid foamed-in-placepolyurethane

14. Warranty period: Minimum TWO years warrantyperiod

15. Operator and service manual essentialrequirement

16. Quality Certification: Only international qualityCE certified product

4. DigitalElectronicPrecision Balance

1. Type – Top loading Precision Balance

2. Fully automatic time and temperature controlledinternal calibration and balance should becapable to adjust itself Auto zero setting.


4. Accuracy: 0.01gm


6. Capacity: 1200gm, Covered type - Glass draftshield with sliding door required..






11. Stabilization Time, 2 Seconds (typically).

12. Calibration certificate from NABL accreditedcalibration laboratory should be supplied alongwith the eqp.

13. Warranty: Minimum 12 months warranty againstall manufacturing defects.


1. Internal Bath (volume) Capacity - 15 Ltr.

2. Should be rounded, seamless double walledstainless steel bath to preventing rust, chemicaldamage and contamination. Powder coating likeepoxy coating exterior for easy cleanup

3. Corrosive resistant stainless steel Gabled dripfree lid

4. Temperature


b) Temperature Accuracy: ± 0.2 °C at 37.0°C

c) Temperature Uniformity: ± 0.5 °C at 37 .0°C

d) Digital LED display for operating status ofTEMP



5. Advanced Microprocessor based Control Panelwith digital display with an accuracy of ±0.5oC

6. Bath consists of two pilot lamp, temperaturecontrol knob and ON/OFF switch to work on220/230 volts AC supplied with stirringarrangement without racks and thermometer..

7. Number and types of racks - tube racks for 15mlx 40 and 50ml tubes x 40 – 2 nos each

8. Low level water sensor. Audible warning safetyalarms should be there for high/low temperaturewarnings, and dry running protection.

9. Instrument should have lift up bath cover; Carrierracks should be given for flasks and test tubesracks.

10. A cock should be provided to facilitate draining ofbath contents.

11. Water bath protective media should be there toprevent contamination and formation of algae.


12. Heating capacity - 2 KW; should have all theaccessories required for the functioning of theequipment.

13. All electrical peripherals required for smoothfunctioning e.g. voltage stabilizer should beprovided with the equipment.



1. Configuration: Multi-chamber: 4 chambered,floor-standing model with Castor wheels

2. Capacity (Individual Chamber volume) 50-60 L x4 chambers

3. Independent Temperature Control of EachChambers

4. A minimum of 2 nos of SS-304 height adjustableracks in each chamber.

5. Temperature range (oC): 25-70 oC, ± 0.2 oCaccuracy and ±0.5 oC uniformity withprogrammable Temperature Control withIllumination

6. Temperature and display of each chamber to becontrolled independently).

7. Independent temperature control system foreach chamber to provide precise temperature


9. Door specification: Solid installed with lock

10. No. of Perforated shelves per chamber minimum2 Nos

11. Digital PID Controller or ProgrammableController

12. Over Temperature Protection, Over CurrentLeakage Breaker


14. Magnetic door closure with positive sealinggasket

15. Suitable on - line UPS (5 KVA) to support theinstrument.

16. Certification: Traceable Temperature Calibrationcertificate for each chamber from NABLAccredited laboratory with IQ/OQ/PQ validation

17. Each equipment should be supplied with multichannel data logger for temperature



7. Hot Air Oven 1. External material: 304 Grade Stainless Steelbody with powder coating.


3. Inner chamber: Stainless steel structure withadjustable minimum 2 shelves.

4. Window: Double layer glass observation windowin front side.

5. Type: Bench Top type (Table top model).

6. Temp. Range: Ambient +10°C to +250°C withtemperature setting accuracy ±0.5 °C with forcedair circulation for temperature uniformity



9. Temperature Protection: Automatic overtemperature alarm based protection system.

10. Timer function: Choice of time (On/Off condition)for automatic setting.

11. Temp. Control: Microprocessor control with LCD/LED display.

12. Convection system: Gentle drying and heatingwith superior temperature uniformity.

13. Document and Installation: Traceable calibrationcertificate from NABL accredited calibration lab.Installation has to be carried by the skilled teamwith IQ, OQ and PQ documents and on sitevalidation to be carried out to ensure properworking of hot Air Oven. as per specification.

14. Certification : Traceable Calibration certificatefrom NABL Accredited laboratory with IQ/OQ/PQvalidation



1. Body should be compact, durable, leak proof andmade of stainless steel /heavy duty plastic

2. Laboratory fumigator dispenser consistentparticle size generation of 5-15 µ, better

3. The blower head should be rust proof inert toFormaldehyde, KMnO4, H2O2 and deliveraerosols uniformly

4. Should be compatible with wide range ofdisinfectant in a closed room.


5. Design- With Wheels, Vortex type. Non rotatingand non closing nozzle.

6. Provided with Cable should be at least 5 metersin length, ISI marked

7. Tank Capacity- 5 liters. Easy clean, detachableand non corrosive for chemical

8. The tank capacity, discharge rate and timer onthe machine should be so that the disinfectantshould be able to disinfect 4000-5000 cubic feetin one cycle of 2 hours (max).



9. Automatedpathogendetection anddetermination

1. System should be a fully automated pathogenscreening system from food samples based onthe principle of ELFA/ELISA .

2. All protocols for sample testing should bevalidated as per FDA/AOAC/ AFNOR/ EU/ISO/DIN specifications.

3. All inoculation strips and all reagents required fortesting to be provided.

4. The system should involve only adding of preenriched sample into individual strips containingall other reagents (enzyme conjugate/ washbuffer/ substrate).

5. The instrument shall be a multi parametricsystem and able to perform more than twoparameters in the same run.

6. System should be supplied with an accessory forsample heating device.

7. System should be capable of detecting andenumerating:



iii) E.coli


v) Campylobacter

vi) Shigella

vii) Vibrio sps

8. System should be supplied with an accessorysystem to determine E.coli, Shigella species,Vibrio species, anaerobic bacteria (Clostridium


species) from food samples based oncolorimetric technology.

9. Negative and Positive reference organisms mustbe supplied with the kits

10. Detection methods must be available in bothkinetic mode and end point mode within a day.

11. The results for the Biochemical reactions shouldbe available on an intuitive software which is 21CFR part 11 compliant with facility of audit trailand electronic signature.

12. Biochemical profiling should be done usingplastic cards impregnated with biochemicalsubstrates specifically for Gram positive cocci,Gram negative cocci, Gram negative rods,Bacillus species, Coryneform species, anaerobicbacteria and yeast species.

13. Biochemical profiling should be done by anautomatic analyzer allowing automatic filling oftest cards with the test suspension followed byautomatic internal barcode reading, sealing andloading of cards in the incubator sections.

14. Analyzer should be connected to a computer withpreloaded software capable of kinetic analysis ofongoing reading and producing results in realtime.

15. Software should be capable of creating neworganism list in the database apart from theexisting database.

16. System should be provided with an accessorysystem to perform automated Gram staining forpositive samples to confirm and further testing.

17. System should be provided with a accessorysystem based on FRET technology(Fluorescence Resonance Energy Transfer)coupled with Melt point peak analysis to detectfood borne pathogens.

18. System should be provided with an accessorywith specific media to detect anaerobic bacteriafrom canned food samples / juices usingcolorimetry technology.

19. All test results should be obtained between 24 –72 hrs.

20. A remote access software should be providedwith the system to help monitoring of the systemremotely and for troubleshooting.


21. System should be accompanied with allaccessories like computer, printer, barcodescanner.

22. System should be supported with MS windowsoperated system and all modular hardware unitswith sample preparation station, reading stationcomputer and accessories with barcode scannerUSB, colour printer and provision for integrationwith LIMS.

23. Software up-gradation should be free of cost forlifetime of system. System should come alongwith the entire necessary accessory and shouldbe ready to work. Any accessory system(s) otherthan those mentioned in the technicalspecifications, that are required for satisfactoryinstallation of the system should be quoted andsupplied with the instrument.

24. The system must have no additional reagentcosts. If additional reagent costs are requiredplease supply details including cost andpreparation time.

25. For validation vender should having it owncapability with their own company trained serviceengineer to perform validation. No third partvalidation will be entertained. One validation atthe time of installation should be done bycompany personnel.


27. Warranty: Warranty should include parts andlabors for 3-years. Warranty should start from theday of installation as per the convenience andagreement with CFL, Kolkata.

28. Kits for pathogen screening and identification for1000 samples may be quoted


1. System should be able to do microbialenumeration from food samples using protocolsin compliance with AOAC/ AFNOR/ ISOmethods.

2. System should be able to perform automatedmicrobial enumeration in food samples usingMPN method in 24 - 48 hrs.

3. System should be able to perform enumerationfor the following parameters with a detection limitup to 4,900,000 CFU/ml or CFU/g:

i) Aerobic count



iii) E.coli counts


v) S.aureus counts




4. System should be able to do automate sampleinoculation.

5. System should be able to do result interpretationautomatically.

6. Kits for test provided for testing should containthe culture medium , containing in a barcodedvial, in dehydrated format and containfluorescent indicator substrate.

7. System should be able to have a throughput ofproviding test results for 300 - 400 tests in 6 hrsgiving results for microbial enumeration.

8. Samples tested on the system should havecomplete traceability with data integrity forresults.

9. System should be supplied with an accessorysystem for automatic gravimetric dilution ofsample preparation along with one pump. Itshould be a self regulating weighing system withdrift alarm with accuracy in compliant with ISO7218 and ISO6887-1.

10. System should be supplied with an accessorysystem for homogenization of sample withflexible speed (slow/normal/fast), blendingcapacity (80 to 400ml) with adjustable timer (10secs to 3mins) and removable stainless steelpaddles, integrated waste drawer, very low noiselevel.

11. System should come along with the entirenecessary accessory and should be ready towork. Any accessory system(s) other than thosementioned in the technical specifications, thatare required for satisfactory installation of thesystem should be quoted and supplied with theinstrument.

12. The system must have no additional reagentcosts. If additional reagent costs are requiredplease supply details including cost andpreparation time.





16. Kits for microbial enumeration may be quoted


The system should be an automated system for bothReal Time PCR and post pcr analysis

HARDWARE:

1. The system should be Peltier based PCRmachine supporting all of the following formats:96-well plate with optical adhesive cover, 96-wellplate with optical flats caps, 8-tubes strips withoptical flat caps.

2. The normalization of reaction due to non-PCRrelated fluctuations should be possible by usingany passive reference dye is essential.

3. The excitation source should be bright whiteLED/Laser/halogen and the detection systemshould be through photodiode/CCD Camera.

4. The built-in emission filters to support a broaderrange of fluorophores with a higher sensitivity forlonger wave length (red dyes). The systemshould be configured and calibrated to use anyof the following dyes or a combination thereof:FAM™,SYBR® Green, VIC®, JOE ™, HEX,TET, BY®, NED ™, TAMRA ™, Cy3®, JUN®,ROX™, TEXAS RED®, and capability ofmultiplexing for five targets or better.

5. The hardware must provide Peltier thermalcycling with pre-configured mode for Fast-PCR(40 cycles in less than 35 minutes) as well asStandard-PCR run in the same block.

6. System must have flexibility of running 2-3different temperatures simultaneously in thesame run with different set of annealingtemperatures in a single run.

7. The system should have temperature range atleast 4 oC-100 oC to facilitate incubation ofsamples at low temperature.

8. The system should have peak block ramp ratefor heating and cooling exceeding 4.6 oC/ second


with temperature uniformity of 0.4 oC or betterand 0.25 Temperature Accuracy. Vendor shouldspecify the sample ramp rate and should bemore than 3.5°C/sec

9. System should support minimum recommendedreaction volume of 10 µL and thermal cyclingconditions to eliminate optimization of PCRconditions for running the templates fromdifferent sources simultaneously although lowerwould be preferred to minimize reagentconsumption.

10. The instrument should have real timequantitative PCR installation specification whichdemonstrates the ability to distinguish between1.5 fold templates copies with a confidence levelequal to 99.5% or better to be demonstrated withRNase P instrument verification plate required tobe done at the time of installation.

11. The system should have preferably TouchScreen LCD feature with real time visuals ofamplification plots etc to avoid dependency oncomputer for operation with USB port.

12. Computer: A business line computer (eithernotebook or tower) for system control, operation,analysis, net-working of multiple systems and aUSB port for data export to Power point, Excel orJPEG file formats with colored laser printer

13. Latest compatible data workstation with allsystem software and monitor should be providedwith the system.

14. Installation specifications must demonstrate theability to detect differences as small as 1.5 foldor better in target quantities

15. IQ/OQ should be provided for the instrument

16. A compatible 2 KVA true online UPS with 30minute backup should be provided along withinstrument.

17. Vendor should provide a complete line ofreagents including 1)Taq Man universal PCRmaster mix (500 reactions) 2)SYBR Greenmaster mixes (500 reaction) and disposablesincluding tubes, 96 well plate for use with thesystem for onsite application training afterinstallation and 3) TAQMAN RNASE P 96-wellinstrument verification plate.

SOFTWARE:


18. Dedicated licensed full version software forprimer and probe design must be included in thesupply.

19. The instrument should have licenced softwarethat can analyze multiple perspectives in theMultiple Plots view, with side by side views of alldata aspects including the amplification plots,standard curve, multi-component data plots, andraw data.

20. The system should also include software tosupport applications including absolutequantitation, Relative quantitation, multiplex-PCR, allelic discrimination (SNP), high resolutionmelt curve analysis as well as pathogendetection and plus/minus assay using internalpositive control.

21. The instrument software should have a multi-componenting algorithm designed to provideprecise deconvolution of multiple dye signals toenable the simultaneous detection of multiplefluorophores,

22. Should be supplied with Software forapplications including absolute quantification,relative quantitation /gene expression/ SNPdetection analysis. Licensed software shouldalso include and supply statistical analysis toolslike Box-Whisker plots to assess Ct distribution,scatter plots and heat maps to assess samplecorrelation and quality

23. The instrument software should haveexperimental design wizard and reaction setupinformation including pipetting protocols.

24. Should support remote monitoring through a webbrowser-based software for accessing andanalysing data anywhere and anytime in the worl

25. The vendor should clearly indicate compliance ordeviation vis –a vis the tender specifications andshould be highlighted in the literature or manuals.

26. The instrument should be UL approved andmanufactured according to ISO 9001 standards.

27. The vendor supplying the instrument shouldhave own application support laboratory in India,preferable in West Bengal for local and efficientafter sales service support.

28. Three years warranty with one year sparereplacement, if required.


29. Suitable on - line UPS (about 2 KVA) is requiredto support the instrument.


1. Camera - CMOS color camera or higher versionDigital Zoom Minimum 28X or higher

2. Resolution - Minimum 1 mega pixels or higher


4. Counting time - 1000 colonies per second ormore



7. Counting - Automatic, with manual control

8. Counting on petri dishes 90mm or higher

9. Counting on pour, Surface plates Yes; Optional– Petrifilms, Chromogenics

10. Data export PDF, JPEG, BMP, PNG and EXCEL


12. Computer system - Laptop with Windows 10, 3GB RAM, Graphics Card, i-5 or higher processor14 Guarantee 3 years

13. Compliance GLP (Good Laboratory Practice) &full traceability






3. Port / Airlock Capacity 30 plates or more viaairlock

4. Porthole System Manual or Instant Access Ports

5. Gas Supplies ANO2/N2 with gas regulator, gasleak detector


7. Airlock Cycle Time Automatic with timer option

8. Automatic Dehumidifier Fitted as standard


9. Desired purity level: H2O< 1 ppm, O2< 1ppm


11. Electrical power: 230 V/50-60 Hz, 10 A or 115 V/ 50-60 Hz, 20 A or 100 V/ 50-60 Hz, 20

12. Glove Ports, Gloves Material: Butyl, thickness0.4 mm or more

13. Dimensions (w/d/h - mm) 1255 / 720 / 710

14. Weight (lbs/kg) 220 / 100

15. Temperature Range 5°C above ambient up to45°C


17. Circulation Unit: Flow rate of around 20 m3 /h(Working gas Nitrogen)


19. Sliding Tray: Stainless steel or other corrosionfree material


1. Ultra pure water system should take at least 100Micro Siemens of Water conductivity and shoulddeliver ultra pure product water by point of usedispenser with rocker arm, volumetric dispensingand auto shut off facility having



iii) TOC level < 10 ppb iv) Flow rate > 1 lit /min

2. Should have separate feed water specificpurification cartridge and application specificpolishing cartridge

3. Should have a vertically placed dual wavelength(185 & 254nm) hotcathode, UV lamp with ballastand quartz sleeve placed in a electro polishedhousing.

4. Final filter 0.22 micron PVDF validatedmembrane. System should have option forproducing Pyrogen/Rnase-free water with UFcartridge.

5. Point of use gun with rocker arm and volumetricand fixed volume dispensing (3% accuracy), witha green LED

6. Built in coaxial resistivity meter with a cellconstant of 0.01/cm and 0.1degree C accuracythermistor


7. Maintenance display for sanitization, exchangepurification cartridges, activation of fast flush,depressurization, air purge

8. Control display showing product water resistivity/ conductivity both compensated and noncompensated mode, product water temperature,product water resistivity greater or below setpoint

15. FullyAutomatedElisa Reader &Washer

A PC based fully automated ELISA Plate reader withdouble beam optics with pre-programmedapplications able to support all plate formats Ubottom, V bottom and flat bottom 8/12/96-well microplates and provision for conventional quartz /glass/plastic cuvettes with all the requiredaccessories.

1. Should have inbuilt Shaker with linear/orbitalmode

2. Should be automatically programmed with on-board touch screen & soft keys

3. Capable of storing method with analysis:> 100method programs on the instrument

4. Detector: Silicon Photodiode dual detector/PMT

a. Wavelength Selection: Wave lengthselection should be doublemonochromatic with 1nm increment

b. Temperature control: Up to 60 C or better

c. Light Source; Halogen lamp for Visiblerange

d. Deuterium Lamp for UV range, lightsource should be auto automaticallyselected as per wavelength required.

e. Scan Ordinate Modes: Absorbance, %Transmittance, % Reflectance



h. Accuracy 1% or ± 0.01 A or better forentire range

i. Repeatability: 05 % ± 0.005 A or better

j. Photometric Range: Absorbance 0-3.0Abs


i. 1A± 0.015A for single wavelength

ii. 2A: ± 0.02A for dual/multiplewavelength


l. Linearity : ± 2 % from 0 to 3.000 A at 405nm

m. Reproducibility: ± 1 % from 0 to 3.000 Aat 405 nm

n. Reading time: < 15 secs for 96 wells

o. Noise: 0.00005 Abs RMS (500nm) orbetter

p. Stability & Drift: Automatic calibrationbetween each plate reading

q. Baseline flatness: ± 0.0005 Abs or better

5. SoftwareCompatible Software should be user friendly &simple for data handling with feature like easy touse report publisher, online help and answerwizard, GLP & audit trail and fully compatible withWindows.System built in features such as real time displayof concentration, photometric mode, single /multi-wavelength.System should have capability to do qualitative,quantitative, kinetics with any formulae includingvalidation, transformation, and factors andfloating cut off.,

6. The software should be 21CFR part 11compliant.

7. Validation Plates for hardware validation ofabsorbance must be provided

8. Plate Incubator

a. Compact Digitally controlled with orbitalshaking

b. Should hold two 96 well microplates, formixing and/or incubating.

c. Speed 400 to 1200 rpm or betterd. Ambient to 40°C with resolution of

0.1°C.e. Digital timer

11. ELISA Microplate Washer:

a. Fully automatic.b. Should Wash flat, round, and V-bottom

plates and stripsc. Automatic calibration, alignment, and last

row detectiond. Should have 2-4 independent liquid channels

e. Wash volume per well should beprogrammable

f. Residual aspiration volume < 2μLg. Auto-water detection of waste and buffers

bottle levels.


h. With Audible alarm when waste bottle is fulland when buffers are empty

13. ELISA Plates: 96 well ELISA Plates 200 Nos

14. Computer and Printer: Latest configurationfactory set branded PC system with 22-23’’ FullHD Monitor with printer –B/W – duplex- laser-legal, A4 - 1200dpi-up to 21 ppm –capacity withnetwork card

15. Suitable UPS with 60 mins backup power forwasher incubator and reader

16. Certificate from an ISO 17025 accreditedcalibration lab for spectral calibration

17. Compliance: IQ/OQ/PQ of instrument andSoftware should be provided along withdocument

18. Operation and training component: The supplierwill have to carry out successful Installation at thelaboratory premises and provide on – sitecomprehensive training to scientific personneloperating the system till customer satisfaction

19. Warranted for 3 years after satisfactoryinstallation and working excluding consumableparts and accessories.


1. Purpose of Equipment: Functions as portablemonitor for use in refrigerators/ Oven/Incubators.

2. It displays and stores data that can bedownloaded to a PC with MS windows supportedsoftware.


4. Accuracy: 0.3˚C


6. Memory Size: 2000 to 2500 Measurements.

7. External Material: Stainless steel/Plastic.


9. Power source: internal lithium battery.

10. Battery life available: 5+ years or 1 millionmeasurements.

11. Reading software and cable needs to beprovided.

12. The equipment quoted should be CE Certified orUSFDA approved.

17. Digital Trinocular Microscope

1. Optical system Infinitely corrected system FocusVertical stage movement 25mm or more for


with imageprocessingsystem anddigital camera

course stroke vertical stage movement 1micronor less for fine stroke Illuminator Lamp house for100 watts halogen lamp with DIC upgradable.

2. Revolving nose piece: Reversed sextuplerevolving nose piece should be upgradable toDIC in future

3. Objectives Plan achromatic 2X N.A 0.06 Planachromatic 4X N.A 0.10 Plane achromatic 10XN.A 0.25 Plane achromatic 40X N.A 0.65 (spring)Plane achromatic 100X N.A 1.25 ( spring & oil)

4. Observation field Wide field trinocular eye piecetube with 10X eye pieces of 25mm or more F.O.V7 Stage Ceramic coated surface mechanicalstage with right hand low drive controlled with lefthand for two specimens.

5. Condenser Swing out condenser usable for 2X-100X.

6. Camera & software Digital pool CCD cameraapprox. 3MP/4MP, with 10 bit digitalization,2048X1500.

7. Software to capture and image processing.

8. Computer system i5 processor, 4GBRAM,500GB HDD, DVR R/ W, TFT 20”.Microscope, camera and software should befrom same manufacturer.


1. Safety Bunsen Burner with flame monitoring andoverheating protection for safe operation.

2. The flame can be rapidly ignited by a footswitchor the push button without the need of a lighteror matches.

3. Two adjustment knobs for air and gas to alloweasy fine-tuning of flame size and temperature.

4. The Safety Bunsen Burner should be compatibleto common gas types such as butane/propaneand natural gas and can be connected to eithera gas distribution system or to different gascartridges.

5. For heating applications or to flame-sterilizenecks of large Erlenmeyer flasks, the SafetyBunsen Burner should be equipped with a longburner head. The quick coupling of the burnerhead

6. The smooth, chrome-plated metal housing iseasy to clean and both UV- and solvent-resistant.



1. Overall internal dimensions (W x D x H):Minimum 62 x 75 x 82 cm

2. Body: Epoxy Powder Coated Steel Chambermade with corrosive resistant stainless steel


4. Temperature Accuracy: ± 0.2 °C at 37 .0°C

5. Temperature Uniformity: ± 0.5 °C at 37 .0°C

6. Shaking Motion: Linear (Reciprocal) Motion withinterchangeable holders for Erlenmeyer flasks(10ml, 25ml, 50ml, 125ml, 250ml, 500ml), testtubes and 1.5-2.0 vials


8. Control: Integrated Microprocessor PID ControlAuto STOP


a. Over-Temperature Cut-Off Alarm(more than 1°C from set point)

b. Over RPM cut-off Alarm (more than 5rpm)

c. Over Current Cut-Off Alarm


10. Digital LED display for operating status of TEMPand RPM


12. Programmable controller offering up to 4 modesof timer and parameter control for reduced userintervention.

13. Timer 0.1 to 99.9 hours or continuous mode



16. Removable bottom plate and shaking insert

17. Clamps and racks 125ml-10nos, 250ml-10nos,500ml- 10nos. Test tube rack for 20x50ml tube-1 no and test tube rack for 42x15ml tubes-1 no.should be quoted.

18. Power requirement: 230V/50-60Hz 16. SuitableServo Voltage Stabilizer should be quoted

19. Warranty should include parts and labors for 3-years. Warranty should start from the day ofinstallation as per the convenience andagreement with CFL, Kolkata.




2. Max. pumping speed at 50/60 Hz 0.7 / 0.85 m3/h

3. Max. pumping speed at 50/60 Hz 0.4 / 0.5 cfm

4. Ultimate vacuum (abs.) 100 / 75 mbar/torr

5. Ambient temperature range (operation) 10 – 40°C

6. Ambient temperature range (storage) -10 – 60 °C





11. Rated motor speed at 50/60 Hz 1500/1800 min-1



a. Chamber of stainless steel with an openingdoor

b. Should have multi-function digital displayProvision of adjustable blending power withon screen indicator.

c. Should have provision of removable paddlesfor cleaning and autoclaving

d. Should have facility for side by side paddlestop.

e. Provision of fully opening door facility foreasy cleaning

2. Disposable bag size: Appropriate to the model &capacity quoted


4. Temperature Ambient operatingtemperature 10-35oC.

5. Humidity range Operating relative humidityrange should be 10-89%

6. Adjustable timer settings 1sec-60 mins.

7. Paddle speed Variable speed (4-10 strokes/sec or better

8. Sensor To ensures immediate stop ofblending in the event of a leakage

9. Accessories Bags (1000 numbers), Bagclips (50 numbers) Bag storage rack/stand (2numbers)

10. Bag sealer


11. Operation and training component Thesupplier will have to carry out successfulInstallation at the laboratory premises andprovide on - site comprehensive training forscientific personnel operating the system tillcustomer satisfaction

12. Warranted for 3 years after satisfactoryinstallation and working excluding consumableparts and accessories


2. Dimensions – Height - 25 cm, Diameter - 11 cm


4. Diameter of petri dish: 90 mm (3½ inches)

5. Nominal Airflow - 100 liters / min. + 2.5%

6. Standard Sampling Volumes - 50, 100, 250, 500,1000 liters

7. Compliance GLP (Good Laboratory Practice) &full traceability







Please quote for both the above options


Inner chamber, washing arms and tank filtersmade of high quality AISI 316 L stainless steelwith HEPA filtered chamber

3. Front Glass Door

Glass Door version – Inside chamber must bevisible, while in washing/drying run.

4. Control System

Soft touch LCD display. Microprocessorcontrolled.



Minimum two automatic internal liquid dispenser


a. At least 10 pre-programmed standardcycles. Including Pre-set programs forchemistry glassware, bacteriology (hightemperature), stubborn stains (agar) andvolumetric glassware (lowertemperature).

b. Additional programs that can be modifiedto fit any.

c. Water rinses for hot, cold and hot/cold DIwater.




Fully adjustable wash temp. up to 90deg C


Must include all external tap water filteringsystem, preferably from local supplier

9. Rack systems and accessories to accommodateall types of glassware (beaker, flask, pipette,petri dish, burette, measuring cylinder, test tubeetc) and laboratory items various configurationsof sizes and quantities of jets or baskets tohandle all types of glass and lab ware frombottles to pipettes.

10. Racks should be interchangeable betweenlevels.


Built in forced air dryer unit for drying entireglassware content after the wash/rinse cycle.

12. Consumables required for washing/ drying cycle

iii) Must provide all necessary washingchemicals for 100 wash run cycle.

iv) All quality washing chemicals must beeasily available in Indian market atreasonable price (Indian Rupees).Imported washing chemicals/consumables are discouraged.

13. Visual and audible alarms in the event of amalfunction, displaying the error



a. IQ/OQ compliance

b. Calibration certificates for temperature


The vendor must carry out the installation andcommissioning at site, including the installationof tap water filter system. The tap water inlet anddrain will be provided at site.

16. Warranty Period

Minimum TWO years full comprehensivewarranty must be provided for all parts in thisequipment.


Necessary end user training and instructionsmust be provided to all users at site.


Must provide the list of users/ customers of thisequipment in India.


iii) Telescopic bearing railing for loading thebasket.

iv) Operator and Service manual with allspare parts list.

20. Availability of all spare parts and service supportin India


1. System A fully automated spectrophotometerwith double beam optics with pre-programmedapplications using conventional quartz /glass/plastic cuvettes with all the requiredaccessories.

2. Operation keys Instrument should operateimmediately after switch on with no warming uptime

3. Should be automatically programmed with on-board touch screen & soft keys

4. Capable to store method with analysis:> 100method programs on the instrument, > 1000results with data, evaluation results and usedparameters

5. Optical Design Double Beam with sample andreference cuvette positions; Czerny-TurnerMonochromatic/Holographic grating with sealedoptics


6. Reference Compartment Should accommodatecells up to 10 mm path length as standard feature

7. Light Source

a. Halogen lamp for Visible range

b. Deuterium Lamp for UV range, lightsource should be auto automaticallyselected as per wavelength required.

8. Detector Silicon Photodiode dual detector/PMT

9. Scan Ordinate Modes Absorbance, %Transmittance, % Reflectance


11. Wavelength Range 180 -1100 nm

12. Wavelength Accuracy ± 0.3nm or better forentire range

13. Wavelength Repeatability ± 0.1nm orbetter

14. Scanning Speed Selectable Variablewavelength scan rate 10nm/min to 2500 nm/minor

15. Spectral Bandwidth Variable(0.1/0.2/0.5/1/2/5) nm


a. Absorbance = -4.5 to 4.5 Abs or better.

b. Transmittance & reflectance 0 to80000 % or better.


a. 0.5 A: ± 0.004A;

b. 1A: ± 0.006A;

c. 2A: ± 0.010A; (440 nm; traceableneutral density filters)

18. Stray Light

a. Max. 0.005% (220 nm NaI) or better,

b. Max. 0.005% (340,370 nm NaNO2) orbetter

c. Max. 1% (198 nm KCI) or better

19. Noise 0.00005 Abs RMS (500nm) or better

20. Drift < 0.0005 A/hr (500 nm, 1 hour warm-up)

21. Baseline flatness ± 0.0005 Abs or better

22. Application Software Compatible Softwareshould be user friendly & simple for data handling


with feature like easy to use report publisher,online help and answer wizard, GLP & audit trailand fully compatible with Windows.

23. System built in features such as real-time displayof concentration, time scan, photometric mode,single/multi-wavelength, capability for eventrecording (e.g., addition of reagents)

24. Software should have built in Methods:

a. Absorbance with one or morewavelengths,

b. Scans, Nucleic acids, Proteins, OD600,

c. Evaluation: via factor, standard andcalibration curve

d. Dual wavelength with subtraction anddivision evaluation

e. Method dependent evaluation:

f. Absorbance, concentration via factorand standard

g. Concentration via standard seriesusing Linear regression, Nonlinearregression with 2nd and 3rd degreepolynomials

h. Spline analysis,

i. Linear interpolation (point to pointevaluation)

j. Absorbance allocation via subtractionand division

k. Ratio 260/280, 260/230, Molarconcentration and total yield fornucleic acids.

25. The software should be 21CFR part 11compliant.


a. One pair each of 0.5, 1 and 3 ml quartzcuvettes 10 mm path length

b. One pair each of 0.5, 1, and 3 ml glasscuvettes 10 mm path length

c. Cuvette holder

d. Deuterium Lamp

e. Halogen lamp

f. Holmium oxide glass filters forwavelength calibration.


g. Didymium glass filter to checkwavelength accuracy

h. NIST traceable Potassium dichromate

27. Computer and printer Latest configurationfactory set branded PC system with 22-23'' FullHD Monitor with printer -B/W - duplex- laser-legal,A4 - 1200dpi-up to 21 ppm -capacity withnetwork card

28. UPS Suitable UPS with 60 mins backuppower

29. Calibration Certificate from an ISO 17025accredited lab for spectral calibration.

30. Compliance IQ/OQ/PQ of instrument andSoftware should be provided along withdocument

31. Operation and training component Thesupplier will have to carry out successfulInstallation at the laboratory premises andprovide on - site comprehensive training forscientific personnel operating the system tillcustomer satisfaction

32. Warranty Warranted for 3 years aftersatisfactory installation and working excludingconsumable parts and accessories.


1. Temperature -20 °C to 60 °C ± 0.5 °C -Readability 0.1 °C

2. R.H. 5 % to 95 % R.H. ± 2.5 % - % R.H readability

3. Backlit dual display of humidity and temperature




26. pH cum ORPMeter

1. pH Range -2.000 to 16.000 pH


3. pH Accuracy (@25ºC/77ºF) ±0.01 pH, ±0.002 pH

4. pH Calibration 5 points (Standard mode) 1.68,4.01 (3.00†), 6.86, 7.01, 9.18, 10.01, 12.45, andtwo custom buffers; 3 points (Basic mode) 4.01;6.86; 7.01; 9.18; 10.01

5. pH Temperature Compensation ATC: -5.0 to100.0ºC; 23.0 to 212.0°F*

6. mV Range ±1000.0 mV; ±2000.0 mV



8. mV Accuracy ±0.2 mV (±999.9 mV); ±1 mV(±2000 mV)




a. Temperature Range -20.0 to 120.0 ºC



d. °C/°F Yes


Glass and reference junction diagnostics ( HI11311 & HI 12301 only), out of calibration range, probe condition, response time

13. GLP Model

14. Logging: up to 1000 records organized in:Manual log-on-demand (Max. 200 logs), Manuallog-on-stability (Max. 200 logs), Interval logging(Max. 600 samples; 100 lots)

15. Connectivity - 1 micro USB port for charging andPC connectivity, 1 USB port for storage

16. Environment - 0 to 50°C (32 to 122°F), RH max95% non-condensing

17. Battery Type/Life - Built-in rechargeable batterywith up to 8 hours of continuous use


19. Dimensions - 202 x 140 x 12.7mm approx


21. Warranty: 2 years including probe 6 months

Total cost of (B)

BUY BACK1. Buy-back price for old Biosafety Cabinet – 4 ft [Make: Amar

Chand & Co., Ambala, India, Year of Installation: 2008]2. Buy-back price for old Fully Automatic Autoclave – 60 lit [Make:

Osworld, Mumbai, India, Year of Installation: 2013]3. Buy-back price for old Precision Balance [Make: Sartorius,

LP1200S Year of Installation: 2007]4. Buy-back price for old BOD Incubator (2 nos.) [Make: YOMA, YORKO

(Double Door) India, Year of Installation: 2009]5. Buy-back price for old Oven [Make: Heraeus Instrument,

Germany, Model T_6 Year of Installation: 2005]6. Buy-back price for old Water Purification System [Make: Millipore,

U.S.A ELIX 3, 10 AND MILLI Q Year of Installation: 2007]


7. Buy-back price for old UV – VIS Spectrophotometer [Make:Varian, Australia CARRY 50 BIO Year of Installation: 1989]Buy Back Total (C)Net Amount (A+B-C)

Note1:(a) The financial bid has to be filled necessarily in the format given above and hasto be signed by the authorized representative of the bidder with full name designationand seal on each page. The above quote should include Clearing and Transportationcharges and cost of necessary civil/electrical work required for installation ofequipments to be carried out by the successful bidder.

(b) This project is a turnkey project. The bidder has to quote price for all theitems mentioned above. In case bidder fails to quote price for all the items his bid willnot be considered for evaluation. Consortium is allowed as a single entity or asubsidiary.

(c) Price quoted should be valid for minimum 06 months from the last date ofsubmission of the bids.

(d) Explanatory notes, if so desired, can be separately submitted along with thefinancial bid but financial bid in the above format is required to be submitted.

(e) Setting up of Microbiology section, supply and installation of equipment time willbe 120 days from the date of issue of Supply order.

(f) Please indicate separately any duties, taxes.Note 2 : The rate may be quoted in foreign currency and/or in Indian currency, however,for comparison/evaluation purpose the bills selling market rate of exchange established byRBI for similar transaction as on date of opening of price bid shall be used to convertforeign currencies to the Indian rupees.

Note 3 : Determination of L-1 will be done based on Net amount (not including levies,taxes and duties levied by Central/State/Local governments such as excise duty, GST,Octroi/entry tax, etc. on final product) of all items/requirements as mentioned above.

Signature of tenderer_______________Name in Block letter _______________Date ____________________________

Capacity in which Signed____________

Sd/-

(Umesh Kumar Jain)Joint Director(QA)

Revised Annexure: II

Technical specification for a Turnkey solution for clean room laboratorySet up & furniture

No Specification QuantityI GENERAL:

The microbiology laboratory shall be modular with unidirectional flow withdifferent zones. A representative zoning floor plan is shown which can besuitably modified by the bidder keeping the flow (personnel and sample)unidirectional and avoiding cross contamination.1. Dress change room (Class D, ISO 8 & < 200 cfu/sq m) over pressure 15

pa2. Clean corridor (Class B, ISO 7 (turbulent) & < 50 cfu/sq m) over

pressure 60 pa3. Sample receiving area (Unclassified)4. Media preparation room (Unclassified)5. Sample preparation room (Class B/ISO 7 & < 50 cfu/sq m) over

pressure 45 pa6. Inoculation room (Class B, ISO 7 & < 50 cfu/sq m) over pressure 45 pa7. Reference culture room (Class B/ISO 7 & < 50 cfu/sq m) over pressure

45 pa8. Incubator and enumeration room (Class D/ISO 8 & < 200 cfu/sq m)9. De-contamination and washing (Unclassified)

The necessary civil and electrical shall be done as per the specifications.The class validation of ‘clean area’ shall be done and report submitted bythe tenderer through a third party accredited agency. Equipment used forvalidation should have valid traceable calibration certificates.The furniture shall be supplied as per the specifications given belowMODULAR PANELLING and FLOORING WORKSThe entire lab as per the layout shall be made with clean room modularpartitions as per the following specification.

1. Wall panels: Pre-fabricated insulated sandwich panels made up of0.8 mm GPSP (Galvanised Plain Skin Pass) GI sheet on both sidewith epoxy polyester powder coating and insulation of PUF withdensity 40±2 Kg/m3. Overall thickness of the panel shall be 80 mm.

2. Cladding panels: Pre-fabricated insulated sandwich panels made upof 0.8mm GPSP GI sheet on both side with epoxy polyester powdercoating and insulation of PUF with density 40±2 Kg/m3. Overallthickness of the panel shall be 40mm.

3. Walkable Ceiling panels: Pre-fabricated insulated sandwich panelsmade up of 0.8mm GPSP GI sheet on both side with epoxypolyester powder coating and insulation of PUF with density 40±2Kg/m3. Overall thickness of the panel shall be 60mm. Panels shallbe designed to fit within each other with self-supported system.Load bearing capacity of the panel shall be 150kg/cu. M. Necessaryclean room lightings and provision for air conditioning outlets shallbe provided.

4. Riser Panels: Pre-fabricated insulated sandwich panels made up of0.8mm GPSP GI sheet on both side with epoxy polyester powder

coating and overall thickness of the panel shall be 80mm withinbuilt riser duct along with perforated grill.

5. Glazed panels flushed view panel with 5mm thick toughened glassof size 900 x 900mm.

6. Aluminium coving: Aluminium coving with radius 50/65 mm withfastening arrangement and aluminium coving corner 3D aluminiumcoving corner 2D.

7. Clean Room Doors: Single Door fit to flush into the wall panels andmust open as shown. Shutter sheet thickness will be 0.8mm andframe will be 1.2mm thick made up of GPSP GI sheet with epoxypolyester powder coating. Leaf thickness will be 44mm and infill willbe PUF with density 40±2 Kg/m3. Door size shall be as perrequirement. Door bottom seal shall be provided.

8. Single Door Accessories:03 Hinges (Altos),01 Door Closer (Altos) -01 Nos. Back to Back Handle01 Nos. Vision (400 x 600) - ,01 Drop Seal01 Lock01 Kick plate

9. Flooring: Seamless antistatic PU floor – Laying 4mm (2+2) thick selfleveling epoxy floor. 2mm screed + 2mm epoxy floor. The existingfloor should be properly cleaned up, surface preparation carried,apply one coat of primer & laid with 2mm thick self-leveling epoxyunpigmented screed floor. And finished with 2mm self-levelingepoxy floor. The floor finish should be 4mm. The self-leveling PUmade of MRF / DUPONT or equivalent. The installed floor shoulddisplay good abrasion resistant & monolithic jointless surface. Shallbe of stain proof, Scratch resistant, Uniform color and free of joints/ undulations / bubbles etc. The floor level shall match with thesurrounding area.

10. Wall to Floor Ceiling – The cove shall be made with silica sand andPU with a radius of 60mm or larger, with all wall / floor joints madeas merging without any unevenness.

11. The panels shall be made of a durable and uniform material thatshould be easy to clean and extremely hygienic.

12. Should not have any sharp edges and corners and do not supportbacteriological or fungicidal growth and is resistant to mostchemicals used in the lab.

13. Gas pipe line shall be provided. The cylinders shall be kept outsideconveniently for replacement.

14. Plumbing lines as required shall be provided. Water drain work withSS GMP TRAP & it’s Connect with main drain line including all civilwork

15. Exhaust line for autoclave, biosafety cabinet, laminar flow andother equipment shall be provided.

16. All temperatures, humidity and pressure should be displayed in thecleancorridor.

17. The switch board should not have any sharp edges18. All doors except the doors in change rooms shall have view panels.

19. Cross over bench shall be provided wherever shown in place ofdoor.

20. The room and sterile corridor over pressure (high positive pressure)should be as indicated above.

21. Fresh air and exhaust should be provided for wash anddecontaminated area.

22. Application of PU Paint on Ceiling & Walls with acrylic pulley base,& Final Finish with two coats for Media preparation area, samplereceipt and decontamination and wash area

23. The bidder should do validation initially while commissioning and 2more validations in an interval of 6 months in a year in the warrantyperiod.

High vacuum system (HVAC) System1.The following area shall be provided with ISO 7 (Class 10,000) withhumidity control HVAC and maintained at 25 ±2 °C

i. Clean corridor over pressure 60 paii. Sample preparation room over pressure 45 pa

iii. Inoculation room over pressure 45 paiv. Reference culture room over pressure 45 pa

The following area shall be provided with unclassified ventilationi. Media preparation room

ii. Sample receipt/storageiii. Incubation and enumeration roomiv. Entry and air showerv. Dress change

vi. Exit2. Overall air quality shall be Class 10000 and should be class 100 at grill

level of HEPA filter. (To achieve this air quality, if any additional itemsare required which are not mentioned in the technical specifications,shall be included in the offer.)i. Validation of HEPA filters by appropriate tests like DOP etc.ii. Air Velocity at outlet of terminal filtration unit / filters.iii. Air Particulate count.iv. Air Change rate calculation.v. Temperature & Humidity test.vi. Pressure differential levels of the OT wrt ambient / adjoining areas.vii. Positive pressure in Pascal as indicated for area

3. Supply, delivery, installation, testing and commissioning of Modulartype floor mounted Double Skin Air Handling Unit of G.S.S. 24 Gaugeducting complete in all respect along with silicon sealant. Duct Sheetmake:- SAIL/Tata/Jindal

4. Application of 12 mm thick XPE TOC Slim insulation Cross Linkedpolyethylene foam with aluminum metalized foil for insulation onSupply duct running inside building area and with UV Foils forinsulation for supply Ducts running out side buildingarea i.e. exposed toatmosphere

5. Application of 09 mm thickness. XPE TOC Slim insulation Cross Linkedpolyethylene foam with aluminum metalized foil for insulation onReturn duct running inside building area and with UV Foils forinsulation for Return Ducts running out side building area i.e. exposedto atmosphere

6. Installation, Testing & Commissioning of powder coated perforated(65%) supply and Return air grills made out of extruded Aluminumsheets (Make:- ISI MARK)

7. Installation, Testing & Commissioning of Powder of suitable numbersand dimensions of coated HEPA Filters ( Efficiency, efficiency 99.99%for 0.3 microns with individual test certificates.) housing with PAO &Pascal Pressure Test Point with canvas connection and VCD.

8. Maximum sound limit in the corridor area shall be 50 to 60 db.9. Installation, Testing & Commissioning of Riser Filters10. Installation of Magnehelic differential Pressure Gauge Make :- DWYER11. Supply, Installation of Central Display Station for Magnehelic

differential Pressure Gauge with negative or positive pressure pipe withSS base plate suitable for 10 Nos .

12. Temperature and RH sensor to measure the temperature and humidityof each clean room. Accuracy levels: Temperature: ± 0.2 °C or better,RH: ± 1% or better.

13. Motor should be non-flame proof type and fan will be non spark prooftype.

14. AHU coil, fan, motor shall be selected for 10% extra capacity.15. The electrical wiring inside the AHU room and interconnection between

AHU and outdoor unit through required protective circuits in allmanners including HP, LP with fully automatic control unit shall beprovided.

16. All the external ducting shall be made weather proof.

OUTDOOR CONDENSING UNITS (Packed ductable split AC)SITC of air cooled condensing units of following capacities with multiplescroll compressor, condenser fan motor unit etc with R-22 refrigerant andMS mounting stand.The capacity shall be decided as per head load calculation. The offeredcapacity shall be mentioned in the offer form. The lab will be functioningfor

i. Supply of R-22 Gas of required quantity.ii. Supply, installation, testing and commissioning of Vibration

Isolators for Condensing Units.iii. Erection, Testing and Commissioning: Ductable Split Unit

Installation,iv. Testing and Commissioning of AHU &ODU along with accessories

like expansion valve, drier and corded remote PCB for temperaturecontrol.

v. Suitable UV lamp for the coil disinfectionElectrical works comprehensive1. The power required for the microbiology lab shall be taken from the

main panel of the building. Necessary distribution panels shall beinstalled by the bidder.

a) Adequate lightings shall be provided.b) The electrical inspectorate’s approval shall be obtained by the

bidderWiring and Accessories

1. Supply & wiring for following points in surface / recessed mountedrigid medium gauge 20mm PVC conduit with all accessories, using 3runs of 1.5 Sq mm FRLS PVC insulated stranded copper conductor

single core wire for phase, neutral & earth, with modular 6A oneway switch, modular plate, suitable GI box etc as required:

2. Light point / exhaust fan / turbo ventilator points as required3. Supply & wiring for circuit / sub main wiring in surface / recessed

mounted rigid medium gauge 25mm PVC conduit with allaccessories in surface/recess

4. Supply and Fixing the following modular type switches &accessories with modular plates and suitable GI boxes and givingnecessary connections as required

i. 6A SP 5 pin shuttered modular type socket with switch ineach switch board

ii. 2 nos 6 A SP 5 pin shuttered modular type socket with 2No’s modular switch –UPS power.

iii. 16A 5 pin shuttered modular type socket with switchiv. Provision for shifting existing switch board to a conventional

location and giving connections etc.v. Supply and fixing 20 amps. 240 volts SP industrial type

socket outlet (IPP) with 2 poles and earth, metal enclosedplug top including supply and fixing of one number 20 amps(10kA) SP MCB (C-Curve) in sheet steel enclosure on surfaceor in recess with chained metal cover for the socket outletand complete with connections testing and commissioningetc. as required.

vi. Installation of Clean Room Lights & Fixture with fitting withLED 12” x 12

vii. Installation & Testing ofa. Modular Switches.b. Modular Sockets for various instruments in each

roomMCBs AND MCB DISTRIBUTION BOARDS

i. Supply and installation of sheet steel, phosphatised and painted,dust and vermin proof enclosure of MCB 4 Way double coverVertical DB – 3 Phase of including copper /brass bus bar, neutrallink, earth bus and DIN rail with MCB/isolator/RCCB etc. fixed onwall using suitable anchor bolts or fixed in recess including cuttinghole on the wall, making good the damages, colour washing etc. asrequired.

ii. Supply and installation of sheet steel, phosphatised and painted,dust and vermin proof enclosure of UPS DB –6 way single Phasedouble cover (IP 42/43)230 V of including copper /brass bus bar,neutral link, earth bus and DIN rail with MCB/isolator etc. fixed onwall using suitable anchor bolts or fixed in recess including cuttinghole on the wall, making good the damages, colour washing etc. asrequired

Wall mounted fansSupply, conveyance, installation, testing and commissioning of wallmounted fans, as required. Fixing necessary bolt and nuts, making goodthe damages etc. as required including giving connections with requiredlength of 24/0.20mm PVC insulated and PVC sheathed 3 core round copperconductor flex wire or with extended original wiring etc. and numbers asrequired.Lighting fixtures

Supply and fixing cast aluminium down light fitting with 11 to 14 W CFL tofalse ceiling including giving connections with required length of16/0.20mm PVCinsulated and PVC sheathed 3 core round copper conductor flex wireconforming to relevant ISS or extending the original wiring and makinggood the surface as required (Wipro WCP 27118 SWG or equivalent make)Validation of HVAC after completion1) Documentation for DQ, IQ, OQ with certificates of all brought items.2) Integrity test for HEPA Filter's once.3) Room Pressure balancing once.4) velocity5) Particle count6) Recovery Test7) Air Flow PatternFire extinguisherSupply and installation of ABC type dry powder fire extinguisher of 2 kg.Capacitycomplete with initial charges and installation brackets15 KVA 3 phase Stand by on-line UPS with 60 minutes back up with battery,rackand stand. Essential lights and equipments shall be connected to the UPS.Air curtain 1.7m length should be installed wherever requiredHand Sanitiser (Automatic IPCA dispenser for clean rooms)1. The hand sanitizer should automatically dispense disinfection (Isopropylalcohol) on to hands.2. The sensor should detect the hand and dispense 0.5ml disinfectantsolution.3. Body should be non-corrosive stainless-steel construction.4. Tank capacity 500ml5. Volume of spray / cycle : 0.5ml

3 Nos

Single Biometric Access control system for restricted entry to the classifiedarea

1No

Installation, Testing & Commissioning SS-316 vertical LAF bench for samplepreparation room as per Size :-4' X 2.5' x 2.5' (2 Nos) meeting

2 Nos

Static Pass boxInstallation, Testing & Commissioning SS-304 static Pass Box fullyautomatic system, with electromagnetic interlocking system, digitaldisplay, UV & fluorescent light alarm system etc.Size :- 1.5' x 1.5' x 1.5'

1 Nos

Dynamic Pass boxInstallation, Testing & Commissioning SS-316 DYNAMIC Pass Box fullyautomatic system, with electromagnetic interlocking system, digitaldisplay, HEPA Filters, UV & fluorescent light alarm system etc.Size :- 1.5' x 1.5' x 1.5'

4 Nos

Cross over Bench1. SS 304, 18 & 16G combination, mat finish2. Bottom side of top provide “C” type stiffner for durability of top3. Inside horizontal support4. Bottom both side 30mm color for will be grouting5. Approx size 1000 mm W x 400 mm D x 600mm H (can be modified tosize)

4 Nos

Modular Work benchInstallation & Commissioning SS304 with drawers and lockersSize - 1500 MM x 750 MM (W) x 900 MM (H) (minor deviations acceptable6 nos of 15/5 amps with 3 pin socket cum Switch with ElectricalPanel should be provided.Table top should be provided with (18mm ±1mm) thick well-polished BlackGraniteShould have reagent storage rack on the top of the table at convenientheight across the table top.Should have provision to keep materials on top of the shelf also.

3

Modular workbench with sink and eyewashStainless steel SS304 table of dimension 1800 x750 (W) x 900 mm (H)tabletop height from floor. Minor deviation in measurement is acceptable.Should have under bench drawers and shutters with lockingarrangement.6 nos of 15/5 amps with 3 pin sockets cum Switch with ElectricalPanel should be provided.Table top should be provided with (18mm ±1mm) thick wellpolished Black Granite.Should have covered reagent storage rack with two shelves on the top ofthe table at convenient height across the table top.7. Should be supplied with one sink at the right end of size 300 x 254 mm(12x10 inches)with two way water tap (hand-free operation) and eyewash.Water connections and plumbing should be provided

2

Movable trolley with lockable wheelsSS 304, 18 & 16G combination, mat finishSize :- 2.5' x 2.5' with two shelf 2nosSize :- 2.5' x 2.5' with Three shelf 2NosBench stoolInstallation & Commissioning SS-304 WORKING STOOL for above benchSS 304, 18 & 16G combination, mat finish2Approximate size 900mm W x 600 mm D x 600mm H

6

Sterile garment storage cabinetDynamic garment storage cubicle complete SS304 construction.One pass through (no recirculation)MINIPLEAT HEPA filters efficiency 99.999% for 0.3 micronPort for HEPA filter leak testingPrefilter 5 microns for fresh air intakeSS rod for hanging folded garments.SS perforated shelves / tray (removable) at bottom for keeping mask andshoecover etc.Stainless steel back panel with perforation at bottom for exhaustFully toughened glass door.Differential pressure gaugesON/OFF switch for blower & white lightsUV light with fittings & limit switchHour meter for UVLeveling legs.Approx internal dimension : 610(W)x 430(D)x 1335(H)mm

1

2017-18 for setting up microbiology section and

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