38166-06(9/27/06)

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Arrowhead education center -Okefenokee Joe

black rat snake: Elaphe obsoleta obsoleta

http://www.okefenokeejoe.com/ http://www.youtube.com/watch?v=_U2knmkrKA0

“Okefenokee Joe sings kin to the wind”

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

2 masses: The masses first appeared in August of 2006 and grew very rapidly. .

Ventral mandible Dorsolateral to the eye

DX: Abscess, granuloma, lymphoma/neoplasia

Surgical excision Histopathology and culturePresented at SEVPAC 2008 – Permission granted for use on SEVPAC

website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Subcutaneous tissue, Granulomas, Multifocal to coalescing, with Intralesional hyphae and arthroconidiating hyphae

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Pantoea agglomorans Enterococcus fecalis Staphylococcus intermedius

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Sabouraud’s Dextrose agar

Mycobiotic agar Incubate at 28-

30oC for four weeks

Examine twice a week for growth

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

“ MYCOLOGY MESS”

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Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Macroscopic Color: wide range Size: wide range Texture: wide range Microscopic- LPCB

Inconsistency in use of terms to describe the microscopic morphology

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Isolate forwarded to The University of Texas Health Science Center at San Antonio for further morphological Characterization

“Chrysosporium anamorph of Nannizziopsis Vriesii”

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

independently populating organism with different morphology: Same Biochemical and physiological properties

Anamorph: asexually propagating thallus Teleomorph: Sexuallly propagating thallus Total organism = Holomorph

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Independently propagating anamorphs

Pseudallescheria boydii Graphium eumorphum Scedosporium apiospermum

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No Matches

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Morphologic charecterization Molecular charecterization

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Chrysosporium ophidiicola

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Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Thanks to VDIL, Bacteriology and Histopathology

section Dr. Deanna Sutton Dr. Melissa Vanmeter Professor Guarro

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Questions?

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