4 - efficient biotechnology for efficient animal nutrition

Platzhalter Titelbild

Let’s make it work!Efficient biotechnology for efficient animal nutrition

Dr. Ralf KelleDecember 4, 2013

Population growthA growing population meansa growing demand for meat, eggs, milk, and fish

Sustainable nutrition Healthy nutrition, with adequate animal protein, that is affordable and available over the long term

Climate

Population

Water

Biodiversity

Resource-efficient animal feeding Limited natural resources must be used as efficiently as possible

December 4, 2013 | R&D press briefing | Efficient biotechnology for efficient animal nutrition

Amino acids are “essential”! Stable trends drive demand

Nutrition

Start-up 2014

Lysine production in Castro

Start-up 2012

Expansion of Midwest Lysine Start-up 2015

Lysine JV in Volgodonsk

Start-up 2013

Agroferm expansion2013-2015

Fermas Technology & Launch Platform

2011-2014

Expansion of biotech R&D


Biotech investment projects inthe Health & Nutrition Business Unit


From production organism to production process

T, pH, shear stress, P/V

pO2 airflow

Concentration of S, P, Mg, Na, K, Fe, Zn, Co, etc.

Quality of water and other raw materials

Byproduct concentration

Product concentration

Osmotic pressure

Feed of C-source, N-source and base/acid

Solubility and oxidation of salts and vitamins

InputProduct formation

Byproduct formation

Growth of organism

Product form

Process efficiency

Crystallization

Viscosity

Foaming

Output

Evaporation

Spray granulation

Biolys®

Fermentation

Mother liquor• residual lysine-HCl• decomposition products • impurities from

raw material source

Cell separation

Ion exchange

Evaporation

Fermentation

Dry L-lysine-HCl

Crystallization

Drying

Wastewater

Ammoniumsulphate

Drybiomass

Drying

Liquid L-Lysine base Wastewater


Biolys® production compared with conventional production

Bacteria + C-source + Ammonia + Sulphuric Acid + Nutrients for Bacteria + Water!

Fructose

Glucose-6-P Fructose-6-P Fructose-1,6-BisP

Fructose-1-PDihydroxyaceton-P

Glycerinaldehyd-3-P

Glycerat-1,3-BisP3-P-Glycerat2-P-GlyceratP-Enolpyruvat

-P

-P-P-P

-P

-P

-P

= O

-P

= O

-P

-P= O

-P= O

Pentosephosphatweg

Phosphotransferase-system (PTS)

glk

ptsIptsHptsM

pgipfkApfk

fdatpi

gap

pgkgpmeno

ppsA

zwf2 devB gnd rpe tal

tkt rpi prsA rbsK pgmA

Pyruvat

= OHOO-

-OOH

AcetylCoA

CitratOxalacetat

AspartatAspartatsemialdehyd

meso-Diaminopimelat

Lysin

Lysin

Homoserin

Threonin

-CoA-CoA

NH3-

-NH3

-OOH

aceElpdlpdA

gltA

acn

icdsucAB

sucCD

fum

mdhmqo

asd lysChom

thrB

dapDdapCdapEdapF

dapAdapB

ddh

lysA

lysIlysG lysE

aceAaceBmez

ppcpck

pyc

Glucose

pykß-Alanin

2-Ketobutyrat

Acetohydroxy-butyrat

Aceto-lactat

Dihydroxymethyl-valerat

Dihydroxy-isovalerat

Isoleucin Valin

Ketomethyl-valerat

Ketoiso-valerat

Pantothenat

panD

coaApanBC

Acetat

poxB

Nicotinat-Biosynthese

Zellwand-Synthese

murE

nadACE

Methionin- S -

metFmetHmetE

metX

ilvBN

ilvC

ilvA

ilvD

ilvE

metBaecD metY

fruK

= O

= O

HOO--OOH-OOH-OOHHOO-

HOO-

-OOH

-OOH

-OOH

= O

-OOH

N

-OOH

= O

-OOH

-OOH

-OOH -OOH

-NH3

NH3 NH3

-NH3

-NH3 sdhABC

ptsIptsHptsF

Lysine

Isoleucine

Isoleucine

Methionine

Valine

Panthotenate

Glutamic Acid


• Amplify biosynthetic pathways

• Slow down side-reactions

• Avoid feed back inhibition

• Balance precursor supply and side reactions

• Enhance net export into the medium

The art of targeted optimization of metabolism

E. coli & C. glutamicum


How can speed and efficiency be optimized?


Good bacterial growth negatively impacts efficiency


Minimized growth is at the expense of process speed


Expanded biotech expertise drives progress in productivity

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1980 1990 2000 2010 2020

Progress, Midwest Lysine2009-2013

Establishment of biotech R&D

Biolys®

production, Fermas1992-2001

Biolys®

production, MWL2000-2008

Mutation and selectionMetabolic engineering and targeted mutation

Functional genomicsSystem biology

Technological development of industrial fermentation= f (yield, product concentration, productivity)


• Continuous improvement in production costs and process efficiency

• Continuous improvement of lysine content at the same level of value using the other components of Biolys®

• Continuous improvement of product properties for use in feed production

Expanded biotech expertise drives progress in productivity

Packaging

Wastewater treatmentTransportThermal energy

Electricity

Raw materials

Process emissions

Carbon source


Increase in efficiency of the Biolys®

production process, 2002-2020

Climate warming potential [kg CO2e/kg]

-0.55 -0.51 -0.44-0.01 -0.01 -0.01

0.41 0.36 0.360.01 0.01 0.01

0.470.35 0.34

1.13

0.93 0.84

0.38

0.35 0.34

0.78

0.730.62

-1,0

-0,5

0,0

0,5

1,0

1,5

2,0

2,5

3,0

3,5

2002 2008 2020


Evonik is working on solutions that go beyond amino acids

Diagnostics for preventing disease and reducing intervention (such as use of antibiotics) in animal nutrition

Probiotics with scientifically proven effect and measurable benefit for use in animal nutrition

Por

tfolio

dev

elop

men

t

Complexity of the technology

Secondary metabolites

Essential fatty acids for aquaculture

L-Valine

L-ThreonineL-Lysine

L-Tryptophan

L-Isoleucine

4 - efficient biotechnology for efficient animal nutrition

Documents