434 PATENT ABSTRACTS

of a polymeric bead or wall of a test tube, the 4859471 other added in solution and labeled by covalent coupling to an enzyme. By reaction between ana- P A N C R E A T I C E N Z Y M E lyte and both antibodies, the enzyme-labeled P R O D U C T S A N D A P R O C E S S antibody becomes fixed to the surface in a quan- tity proportional to the quantity of the analyte. F O R T H E P R E P A R A T I O N After washing sufficiently to remove unreacted T H E R E O F enzyme-labeled antibody, fixed enzyme activity is measured by incubation with a substrate and Werner Fulberth, Hans-Georg Freuer, D 6230 measurement of the rate of the reaction cata- Frankfurt am Main 80, Federal Republic Of lyzed. Fixation of the enzyme causes the reaction Germany products to be localized near the surface. To measure the, concentration of reactant or pro- A process for the preparation of pancreatic en- duct repeatedly during the reaction, the solution zyme products in compressed form is described. must be mixed before each measurement, which A water-based insulating layer and a final lac- can interfere with the measurement. In the prior quer based on organic solvents are applied to the art, the reaction is stopped after incubation and core which contains the active compounds. the product measured once. The method and ap- paratus disclosed here provides stirring and measurement away from the surface, and thus permits repeated measurement during the reac- tion. This kinetic assay can be performed more rapidly and sensitively than assays based on a 4859589

single measurement. E N Z Y M A T I C M E T H O D F O R

P R E P A R A T I O N O F E P O X Y C O M P O U N D S

Sven E Godtfredsen, Fredri Bjorkling, Vaerlose, Denmark assigned to Novo Industti A/S

An epoxy group in a molecule can enzymatically be transferred to another molecule, thereby syn- thesizing carbohydrates carrying an epoxy

4857461 group in the aglycone position.

C O N T I N U O U S P R O C E S S F O R T H E E N Z Y M A T I C P R E P A R A T I O N

O F I S O M A L T U L O S E

4859590 Peter Egerer, W u l f Crueger, Gunter Schmidt- Kastner, Kirschseeorl, Federal Republic Of Ger- E N Z Y M A T I C S Y N T H E S I S O F many assigned to Bayer Aktiengesellschaft C Y C L O D E X T R I N S U S I N G

The present invention relates to a continuous A L P H A - G L U C O S Y L F L U O R I D E process for the enzymatic preparation ofisomal- A S S U B S T R A T E F O R tulose. A periplasmatic sucrose-mutase is pro- C Y C L O D E X T R I N A L P H A ( I R I G H T duced by fermentation of microorganisms which A R R O W 4 ) form sucrose-mutase. The cell-free crude enzyme G L U C O S Y L T R A N S F E R A S E extract is prepared by digestion of the cells and by cross-flow microfiltration. In a single-stage, Joachim Thiem, Wolfgang Treder, Reinhold simultaneous purification and immobilization of Keller, Merten Schlingmann, Mfederal Republic the sucrose-mutase from the cell-free crude Of Germana assigned to Hoechst Aktiengesel- extract conditioned by diafiltration is obtained lschaft by selective bonding to an anionizable cartier matrix. Direct conversion of sucrose into isomal- alpha-Glucosyl fluoride can be converted in the tulose is produced by the sucrose-mutase bonded presence of cyclodextrin alpha(l tight arrow4) to the anionizable cartier matrix, preferably in glucosyltransferase into a mixture of alpha- and cartridge or cartouche form. beta-cyclodextrins in high yield.