PATENT ABSTRACTS

nding, detecting expression, drug screening, and treatment involving the human 5-HTID recep- tor.

5156955

N U C L E O S I D E O X I D A S E A N D A S S A Y M E T H O D U T I L I Z I N G

S A M E

Yoshikaz Isono, Masami Hoshino, Otsu, Japan assigned to Otsuka Foods Co Ltd

The present invention provides a novel nucleoside oxidase YT-1 serving as an enzyme for catalyzing oxidation reaction ofa nucleoside, and oxidase being characterized in that the ox- idase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'- earboxylic acid via a nucleoside-5'-aldehyde without producing hydrogen peroxide, a process for producing the enzyme, a novel micro- organism having ability to produce the enzyme, and a novel analysis or assay method for nucleosides and the like utilizing the enzyme.

5156957

F O L L I C L E S T I M U L A T I N G H O R M O N E

Vemuri Reddy, Nancy Hsiung, Anton K Beck, Edward G Berstine assigned to Genzyme Cor- poration

Biologically active heterodimeric human FSH composed of an alpha subunit and a beta sub- unit, each subunit being synthesized by a cell having an expression vector containing hetero- Iogous DNA encoding the subunit.

133

membrane protein of Bordetella pertussis. The invention also relates to host cells and vectors comprising the nucleotide sequence, as well as a vaccine composition comprising the substan- tially pure protein.

5156959

M E T H O D T O E X P O R T G E N E P R O D U C T S T O T H E G R O W T H

M E D I U M O F G R A M N E G A T I V E B A C T E R I A

Lars Abrahmsen, Tomas Moks, Bjorn Nilsson, Mathias Uhlen, Stockholm, Sweden assigned to KabiGen AB

A process for expressing proteins in Gram(-) bacteria and providing for extracellular secre- tion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired pro- tein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extraceUularly secreted protein; a recombinant DNA construc- tion comprising: a promoter, a signal sequence and a structural gene including a cleavage re- gion, wherein the structural gene is of the formula: See Patent for Tabular Presentation PS where n is an integer ) or = 1, m is an integer, Y is a DNA sequence encoding a desired protein and E and B are the genes corresponding to the pro- tein A regions E and B, with the proviso that the structural gene is different from that encoding natural protein A; a plasmid vector comprising such recombinant DNA construction; a Gram(-) bacterium harbouring said recombinant DNA construction; and proteins obtained by such pro- cess.

5156958

G E N E E N C O D I N G A 30 K I L O D A L T O N O U T E R

MEMBRANE P R O T E I N O F B O R D E T E L L A P E R T U S S I S A N D

M E T H O D O F R E C O M B I N A N T P R O D U C T I O N O F S A I D P R O T E I N

Patricia A Reilly assigned to American Cyanamid Company

The present invention relates to a nucleotide and amino acid sequence of a 30 kilodalton outer

5156968

P U R I F I E D Y E A S T U B I Q U I T I N H Y D R O L A S E

Chung-Cheng Liu assigned to Genentech Inc

Ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition, which hydrolase hydrolyzes a ubiquitin-polypeptide conjugate at the amide bond linking the ubi- quitin and polypeptide, thereby yielding intact polypeptide with an unconjugated, mature N-