5174993

PATENT ABSTRACTS

R E C O M B I N A N T A V I P O X V I R U S A N D I M M U N O L O G I C A L U S E

T H E R E O F

Enzo Paoletti assigned to Health Research Inc

The present invention provides a method for in- ducing an immunological response in a ver- tebrate to a pathogen by inoculating the vertebrate with a synthetic recombinant avipox virus modified by the presence, in a non-essential region of the avipox genome, of DNA from any source which codes for and expresses an antigen of the pathogen. The present invention further provides a synthetic recombinant avipox virus modified by the insertion therein of DNA from any source, and particularly from a non-avipox source, into a non-essential region of the avipox genome.

325

Utilizing mouse monoclonal antibodies which recognize Rodgers 1 and Chido 1 epitopes car- ried on the C4A and C4B molecules, and heat ag- gregated IgG to activate CI, an immunoassay was developed for the quantitation of comple- ment components, including total C4, C4A and C4B. Interassay variation was 12.4%, 11.5% and 10.8%, respectively. The immunoassay was com- pared to the quantitation of total C4 by radial immunodiffusion by testing 103 random white controls and gave a Pearson's product moment correlation coefficient of 0.81. Three genetic total C4 deficient individuals were nonreactive in all three assays. This activated assay is specific, reproducible and superior to existing methods for the quantitation of C4A and C4B and detec- tion of the heterozygous C4 null states.

5175095

O V A R Y - T I S S U E T R A N S C R I P T I O N A L F A C T O R S

5175082

M E T H O D O F C H A R A C T E R I Z I N G G E N O M I C D N A

Alec J Jeffreys, Leicester, United Kingdom as- signed to Imperial Chemical Industries PLC

Test samples of genomic DNA may be charac- terized by the use of polynucleotide probes each of which is specific for an informative genetic locus. Such probes may be prepared by the use of probes which are capable of differentiating DNA by reference to more than one poly- morphic minisatellite region or hypervariable locus. The polynucleotides and probes of the in- vention are of use for genetic identification pur- poses, paternity and maternity testing and particularly in forensic medicine.

Belinda M Martineau, Catherine Houck as- signed to Calgene Inc

Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of trans- cription of a DNA sequence of interest in ovary tissue, particularly in very early fruit develop- ment. The DNA constructs comprise a trans- criptional initiation regulatory region associated with gene expression in ovary tissue from im- mediately prior to anthesis through'flower senes- cence.

5175096

D N A E N C O D I N G A F I B R O N E C T I N B I N D I N G P R O T E I N AS W E L L AS

I T S P R E P A R A T I O N

5175083

I M M U N O A S S A Y F O R T H E Q U A N T I T A T I O N O F H U M A N C4

G E N E P R O D U C T S

Joann M Moulds assigned to Board of Regents University of Texas

k Magnus Ho, Klas Jonsson, Kjell M Lindberg, Christer Signas assigned to Alfa-Laval Agri International Aktiebolag

The present invention relates to a new recom- binant hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein, or polypeptide, having fibronectin bi- nding properties.