96-Well PlateVacuum Manifold Instructions

2

96-Well Plate Vacuum

Manifold

Instructions

Please read the following information first regarding the use of

the Strata® 96-Well Plate Acrylic Manifolds, Phenomenex part

numbers AH0-7284 and AH0-8950.

The Strata 96-Well Plate Acrylic Manifolds (part numbers AH0-7284

and AH0-8950) are packaged with two different height spacers, or

shims, which are designed to be inserted into the manifold base

below the collection plate during use of the manifold. The spacers,

or shims, are necessary to ensure that the bottom of the tips of the

upper 96-well plate extend a small distance into the top of each

well of the lower 96-well collection plate thereby minimizing any

chance of well-to-well cross-contamination.

One spacer is a thin 2 mm silicone rubber sheet for use with the

following collection plates from Phenomenex:• AH0-7194 Strata96-WellCollectionPlate2mL/Conical,

Polypropylene

• AH0-8635 Strata96-WellCollectionPlate, 2mLSquare/Round-Conical,Polypropylene

• AH0-8636 Strata96-WellCollectionPlate, 2mLRound/Round,8mm,Polypropylene

• AH0-7193 Strata96-WellCollectionPlate1mL/Conical, Polypropylene

• AH0-7192 Strata96-WellCollectionPlate350µL/Conical, Polypropylene

The second spacer is a white 3.5 mm polyethylene spacer for use

with the following collection plate from Phenomenex:• AH0-7279 Strata96-WellCollectionPlate1mLRound,

7mm,Polypropylene

STOP: Important Information

3

96-W

ell P

late

Vac

uum

Man

ifold

Inst

ruct

ions

Continued

InStRuCtIonS

Insert the appropriate spacer into the base of the manifold prior to

inserting the collection plate.

PRACtICALnote

It is strongly recommended to use collection plates supplied by

Phenomenex to ensure a proper fit and compatibility with com-

monly used SPE solvents. As a practical matter, it is very difficult

and at times, nearly impossible to actually see if the tips of the 96-

well plate are correctly entering the collection plate for reducing

or eliminating the potential for any cross-contamination. Phenom-

enex plates are guaranteed to fit properly and be compatible with

common SPE elution solvents.

For additional assistance please contact a Phenomenex Technical

Consultant or Sample Preparation Specialist at Phenomenex or

your local distributor.

© 2011 Phenomenex, Inc. All rights reserved.

STOP: Important Information

4

96-Well Plate Vacuum

Manifold

Instructions

The Phenomenex Strata® 96-Well Plate Vacuum Manifold is precision machined from crystal

clear acrylic (top plate) and acetal polymer (plenum chamber). The acrylic top plate allows visu-

al access to the plenum chamber for checking progress on the separation process. The acetal

plenum chamber has medium resistance to alcohols, weak acids, etc., but should be cleaned as

soon as possible if the above solutions are spilt on it. Each unit comes fitted with a needle valve

controller to ensure accurate adjustment of vacuum into the manifold. The unit will also take a

reservoir tray to collect extraneous sample and wash solution for removal and disposal before

collection plates are installed.

DeSCRIPtIon

Lowerplate, comprising of plenum (vacuum) chamber with lever-operated gate valve and needle control valves (part no. AH0-8950 also includes a vacuum gauge). Fitted with cus-tom O-ring in upper surface allowing airtight interface between plates during operation. The plenum chamber can be used either as a simple sump for waste filtrate using reser-voir trays or as a receptacle for a standard 96-well microtiter plate (collection plate). upper plate, comprising machined recess, to fit Phenomenex well plates, with custom polyether foam gasket (AH0-7284) or custom neoprene gasket (AH0-8950).

oPeRAtIonRemove top plate and install reservoir tray. Replace top plate and place well plate on gasket on top plate. Once sorbent washing is completed, remove top plate (with well plate in place). Remove reservoir tray and discard. Place required collection plate in plenum chamber (spacers may be required). Replace top plate, ensuring well plate drain spouts are inline with collection plate wells.

CoLLeCtIonPLAteoPtIonS350 μL, 1 mL, or 2 mL well collection platesPhenomenex offers three types of standard sized square-well plates to fit the vacuum manifold (350 μL, 1 mL, or 2 mL). In addition, we also have 1 mL and 2 mL round well collection plates. These plates will fit into the plenum chamber directly. If round-well ver-sions of the 1 mL well plate are used, a spacer (supplied with manifold kits AH0-7284 and AH0-8950) must be placed at the bottom of the plenum chamber before inserting the collection plate/rack.

96-Well Plate Vacuum Manifold

(Please refer to “Important Information” on pp. 1-2)

5

96-W

ell P

late

Vac

uum

Man

ifold

Inst

ruct

ionsVACuumContRoL

The vacuum control system consists of an on-off valve to the right of the T-connector and a needle valve, located to the left of the T-connector, to adjust the amount of vacuum applied to the chamber. The knurled control knob on the needle valve adjuster should be turned clockwise to a fully closed position. The vacuum source should be connected to the on-off valve and the valve opened (tap parallel to on-off valve). Carefully wind the needle valve controller counter-clockwise until the required level of vacuum is attained. Vacuum can be shut off at any time by turning the on-off tap at right angles to the valve. The knurled control knob can be used to ‘vent’ the plenum chamber, releasing vacuum. This is also a way to reduce vacuum intensity.

mAIntenAnCe&CLeAnIngTo clean, simply wipe all components with a damp paper towel and dry. DO NOT USE AL-COHOLS OR SOLVENTS TO CLEAN COM-PONENTS, USE ONLY WATER. If alcohols or solvents are spilled on the clear acrylic top plate, rinse immediately with water. To ensure the sealing rings and gasket are not contaminated, rinse with water and dry with paper towels. The vacuum unit should not be autoclaved or subjected to high temperature. A spare top gasket is enclosed and others are available from Phenomenex on request. Should it be necessary to return the unit for any reason, call your Phenomenex represen-tative FIRST to obtain a Return Authorization Number. If contaminated by radiation, units will not be accepted for return.

6

96-Well Plate Vacuum

Manifold

Instructions

note: The steps below are generalized and may not com-pletely cover the steps required for your method. Contact your Phenomenex Sample Preparation Specialist for further technical assistance or method development suggestions.

SteP1Insert a reservoir tray in the plenum chamber, then place the well plate on the top of the vacuum manifold. Condition the well plate by passing volumes of appropriate solvent through the columns, followed by a volume of liquid similar in nature to the sample.

SteP2The samples should now be added to the well plate columns, thus allowing all sample components to become retained on the sor-bent surface.

SteP3The interfering components can now be re-moved by washing with the most optimal/appropriate solvents while the analyte is re-tained in the sorbent, allowing the solvents to drop into the reservoir tray as waste. note: This fraction can also be saved using a 96-well col-lection plate instead for further analysis or for expediting troubleshooting concerning analyte retention or wash op-timization during method development.

SteP4Once the interferences have been removed, the reservoir tray should be removed from the plenum chamber and the collection plate can be inserted into the lower section of the manifold. Please ensure that you have the correct size collection plate in the manifold (drain spouts of the 96-well plate should intrude into the tops of the collec-tion plate wells).

Instructions

Collection platewith 96-individual wells

Strata well plate

Waste reservoir(without individual wells)

7

96-W

ell P

late

Vac

uum

Man

ifold

Inst

ruct

ions

SteP5The samples (analyte) can now be eluted off the well plate into the collection plate by washing with a specific solvent that disrupts the analyte-sorbent interactions.note: Please call your Sample Preparation Specialist for assistance to obtain the most optimal results.

SteP6Finally the collection plate containing the analyte can be removed from the vacuum manifold.

ImPoRtAnt Vacuum Information: The vacuum should only be spar-

ingly applied after each reagent and (or) sample has been added to

the complete block. It is recommended that the vacuum should be

pulsed, used sparingly and in some cases not used at all, leaving grav-

ity to take effect especially when using well plates with small sorbent

bed mass wells. Note: Low vacuum settings, longer analyte-sorbent

contact time, and slower flow rates typically yield superior results.

ForoptimalResultsortoImproveYourResults:

8

96-Well Plate Vacuum

Manifold

Instructions

Ordering Information

96-WeLLPLAteSStrata® 96-Well Plates (2 Plates/Box)Phase 25 mg 50 mg 100 mg

SDB-L — 8E-S014-DGB —C18-E 8E-S001-CGB 8E-S001-DGB 8E-S001-EGBC18-U — 8E-S002-DGB —C8 8E-S005-CGB 8E-S005-DGB 8E-S005-EGBPhenyl — 8E-S006-DGB 8E-S006-EGBNH2 8E-S009-CGB 8E-S009-DGB 8E-S009-EGBWCX 8E-S027-CGB 8E-S027-DGB 8E-S027-EGBSCX 8E-S010-CGB — 8E-S010-EGBSAX 8E-S008-CGB 8E-S008-DGB 8E-S008-EGBScreen-A 8E-S019-CGB — —Silica — — 8E-S012-EGB

HIgH-tHRougHPut96-WeLLPLAteSStrata™-X 96-Well Plates (2 Plates/Box)Phase 10 mg 30 mg 60 mg

Strata-X 8E-S100-AGB 8E-S100-TGB 8E-S100-UGBStrata-X-C 8E-S029-AGB 8E-S029-TGB 8E-S029-UGBStrata-X-CW 8E-S035-AGB 8E-S035-TGB 8E-S035-UGBStrata-X-A 8E-S123-AGB 8E-S123-TGB 8E-S123-UGBStrata-X-AW 8E-S038-AGB 8E-S038-TGB 8E-S038-UGBStrata-XL — 8E-S043-TGB —Strata-XL-C — 8E-S044-TGB —Strata-XL-CW — 8E-S052-TGB —Strata-XL-A — 8E-S053-TGB —Strata-XL-AW — 8E-S051-TGB —Strata-X-Drug B 8E-S128-AGB 8E-S128-TGB 8E-S128-UGBStrata-X-Drug N 8E-S129-AGB 8E-S129-TGB 8E-S129-UGB

SAmPLePRePARAtIonmetHoDDeVeLoPmentPLAteS96-Well Plate Kits for High-Throughput ScreeningPart No. Description Unit

KS0-8241 Strata-X 10 mg/well (3 rows)Strata-X-C 10 mg/well (3 rows) Strata-X-CW 10 mg/well (3 rows) Strata-X-AW 10 mg/well (3 rows)

ea

KS0-8209 Strata-X 30 mg/well (3 rows)Strata-X-C 30 mg/well (3 rows) Strata-X-CW 30 mg/well (3 rows)Strata-X-AW 30 mg/well (3 rows)

ea

If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, return the product with comparative data within 45 days for a FULL REFUND.

Call your Technical Consultant or Sample Preparation Specialist for more information or to inquire about other phases, dimensions, or formats.

9

96-W

ell P

late

Vac

uum

Man

ifold

Inst

ruct

ions

StRAtA®ImPACt™:RAPIDPRoteInPReCIPItAtIonWItHoutCentRIFugAtIon

• effectivelyretainsacetonitrile,resultinginleak-free proteinprecipitation

• Directin-plateprecipitationreducestransfersteps forfasterprecipitation

• Wellplateformatallowsforhigh-throughput precipitationviavacuummanifoldorliquidhandlingsystem

Strata Impact Precipitation PlatesPart No. Description Unit/Box

CE0-7565 Strata Impact Protein Precipitation, Square Well, Filter Plate, 2 mL

2

Starter Kit for Protein PrecipitationCE0-8201 Strata Impact Protein Precipitation Plate (2 ea)

Collection Plate 2 mL (2 ea) Sealing Mat, Santoprene™ (2 ea)

ea

FILtRAtIonPLAte:eFFeCtIVeFILtRAtIonFoRVARIouSSAmPLemAtRICeS

•Availablein0.45µmmembraneporosity

•Inertsurfaceeliminatesnon-specificbindingformaximizedresults

•Costeffectivesolutiontomeetallfiltrationgoals

Filtration PlatesPart No. Description Unit/Box

AF0-8265 0.45 µm Glass Fiber 96-Well Filtration Plate 2

SeALIngmAtSAnDtAPe• FitsallPhenomenex96-wellplates,square-wellcollection

plates,round-wellcollectionplates,proteinprecipitationplates,andfiltrationplates

• PierceableandPre-Slitavailable

Sealing Mats*Part No. Description Unit/Box

AH0-8597 Sealing Mats, Pierceable, 96-Square Well, Silicone 50/pkAH0-8598 Sealing Mats, Pre-Slit, 96-Square Well, Silicone 50/pkAH0-8631*** Sealing Mats, Pierceable, 96-Round Well 7mm, Silicone 50/pkAH0-8632*** Sealing Mats, Pre-Slit, 96-Round Well 7mm, Silicone 50/pkAH0-8633** Sealing Mats, Pierceable, 96-Round Well 8mm, Silicone 50/pkAH0-8634** Sealing Mats, Pre-Slit, 96-Round Well 8mm, Silicone 50/pkAH0-8199 Sealing Mats, Pierceable, 96 Square Well, Santoprene™ 100/pkAH0-7195 Sealing Mats, Pierceable, 96-Square Well, Ethylene Vinyl Acetate (EVA) 50/pkAH0-7632 Sealing Tape Pad 10/pk*Square well sealing mats compatible with 2 mL Strata Impact plates, Strata and Strata-X 96-well plates, and 96 square well collection plates.**8 mm round-well sealing mats compatible with 2 mL round-well 8 mm collection plates (AH0-8636)***7 mm round-well sealing mats compatible with1 mL round-well 7 mm collection plates (AH0-7279)

(AH0-7195)

10

96-Well Plate Vacuum

Manifold

Instructions

Ordering Information(Cont’d)

ACCeSSoRIeSFoRHIgH-tHRougHPutPRoteInPReCIPItAtIon,FILtRAtIon,AnDLIquID-LIquIDextRACtIon

Collection Plates • AvailableinconicalV-andround-bottomformats

• madeofchemicallyinertpolypropylene

• Availablein350μL,1and2mLvolumes

Conical V- and round-bottom for maximized sample delivery

Collection Plates*Part No. Descritpion Unit

AH0-7192 Strata 96-Well Collection Plate 350 uL Conical, Polypropylene 50/pk

AH0-7193 Strata 96-Well Collection Plate 1 mL Conical, Polypropylene 50/pkAH0-7279*** Strata 96-Well Collection Plate 1 mL Round, 7 mm, Polypropylene 50/pkAH0-7194 Strata 96-Well Collection Plate 2 mL Conical, Polypropylene 50/pkAH0-8635 Strata 96-Well Collection Plate 2 mL Square/Round-Conical, Polypropylene 50/pk

AH0-8636** Strata 96-Well Collection Plate 2 mL Round/Round, 8 mm, Polypropylene 50/pk

*Square well sealing mats compatible with 2 mL Strata Impact plates, Strata and Strata-X 96-well plates, and 96 square well collection plates.**8 mm round-well sealing mats compatible with 2 mL round-well 8 mm collection plates (AH0-8636)

***7 mm round-well sealing mats compatible with1 mL round-well 7 mm collection plates (AH0-7279)

(AH0-7194) and (AH0-8635)2 mL Square Well

(AH0-7192)350 μL Square Well

(AH0-7193)1 mL Square Well

(AH0-7279)1 mL Round Well

96-W

ell P

late

Vac

uum

Man

ifold

Inst

ruct

ions

11

96-WeLLPLAteVACuummAnIFoLD• Includesvacuumvalveattachmentandtwocollectionplatespacerinserts

• madeofdurableacrylic

• Designedtoaccommodate96-wellplates,collection plates,proteinprecipitationplates,andfiltrationplates

Manifold*Part No. Descritpion Unit

AH0-7284 96-Well Plate Manifold, Acrylic ea

AH0-8950 Strata 96-Well Plate Manifold, Universal w/vacuum gauge eaReplacement Parts and AccessoriesPart No. Descritpion Unit

AHO-7285 96-Well Plate Manifold Replacement Gasket, Flat (to fit between acrylic chamber and 96-well plate), black

ea

AH0-7198 96-Well Plate Manifold Replacement Gasket, Profile (to fit between acrylic chamber and manifold base), white

ea

AH0-8637 Reservoir, Single-Well, High Profile, 96 Bottom Troughs 25/pk*Manifold, compatible with 2 mL Strata Impact plate, Strata and Strata-X 96-well plate formats.

Well Plate

Acrylic Chamber (manifold top)

Manifold Base Plate (houses collection plate)

+

+

=

96-Well PlateVacuum Manifold Instructions

Trademarks Strata is a registered trademark of Phenomenex, Inc. Strata-X and Impact are trademarks of Phenomenex, Inc. Santoprene is a trademark of Exxon Mobil Corporation.

Disclaimer Strata-X is patented by Phenomenex. Subject to Phenomenex Standard Terms and Conditions, which may be viewed at www.phenomenex.com/termsandconditions.

© 2011 Phenomenex, Inc. All rights reserved

IL60

5707

11

Phenomenex products are available worldwide.

support@phenomenex.comwww.phenomenex.com

Continued