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BioSep-SEC-S

HPLC Columns forGel Filtration / Size Exclusion Chromatography

Tired of paying high prices?

Irritated with slow delivery times?

Frustrated by irreproducible results?

Eliminate your problems by using

BioSep for your GFC/ SEC separations.

HPLC columns for gel filtration / size exclusion chromatography

102 103 104 105 106 107

DenaturedUnderGnHCl

DenaturedUnder

SDS

Native

Molecular Weight (Daltons)

3“ x 3.5” Molecular Weight Separation

S-4000

S-4000

S-4000

S-3000

S-2000

S-3000S-2000

S-3000

S-2000

Silica-based GFC / SEC columns for the separation of protein & peptide mixtures

For the analysis of antibodies, protein complexes, protein aggregation, and for desalting

Maintain biological activity of proteins

3 phase options to successfully separate samples of varying molecular weights

Excellent column-to-column reproducibility

Economical pricing

Easy Column Selection with BioSep

Selecting the appropriate column for gel filtration/ size exclusion applications is not always straightforward. Sample details, such as molecular weight and native vs. denatured state, dictate the suitable phase to use. The chart below clearly illustrates which of the 3 BioSep phase options to use based on the characteristics of your sample mixture.

BioSep SEC-S4000Bio-Rad

Bio-Sil® SEC 400

Agilent Technologies

–

other manufacturers

GFC 4000 phases


Bio-Sil® SEC 250


Zorbax® GF-450

other manufacturers

GFC 3000 phases


Bio-Sil® SEC 125


Zorbax® GF-250

other manufacturers

GFC 2000 phases

01

BioSep™- SEC-S

Based on SizeMolecular Weight of your Sample Mixture

Molecular Weight Separation Range for Proteins BioSep-SEC-S

1 Based on a GuaranteedAlternative to the Column You Currently Use

Column Cross Reference Chart

2

• • • Refer to page 5



Phenomenex Tel: 310-212-0555 Fax: 310-328-7768 www.phenomenex.com Phenomenex Tel: 310-212-0555 Fax: 310-328-7768 www.phenomenex.com

Increase Efficiency with BioSep™

How well one protein of interest is separated from another is related to the efficiency of a column; the higher the efficiency the greater the baseline separation between the analytes. As shown in the chart below, BioSep has a very high efficiency, thus making it an excellent column for protein separations via gel filtration/size exclusion.

If BioSep™ does not provide you with equal or better separations than any other GFC column of similar porosity, type and comparable dimensions, send in your comparative data within 45 days and keep the BioSep™

for FREE.

02

-400001000060000

110000160000210000260000

mAU

4 5 6 7 8 9 10 11 12 13 min

2 1

3

4 5

* for 316 stainless steel

BioSep-SEC-S2000

Column: BioSep-SEC-S3000 Dimensions: 300 x 7.8 mm Part No.: 00H-2146-K0 Mobile Phase: 100 mM Potassium phosphate buffer, pH 6.8 Flow Rate: 1 mL/min Detection: UV @ 280 nm Temperature: 22 °C Sample: 1. Thyroglobulin 4. Myoglobin 2. Gammaglobulin 5. Uridine 3. Ovalbumin

App ID 15465

Proteins on BioSep-SEC-S3000

“I have been using the BioSep 3000 size exclusion columns for the past five years to purify radioiodinated antibodies. These columns have provided us with consistent, reproducible results for a very reasonable and competitive price. In addition, the customer service and technical support provided by Phenomenex is unlike that of any other vendor that I deal with and is truly commendable and a selling point in itself. I would highly recommend this column.Pam Zaller-Farrington - SolidPhase Inc. ’’

BioSep-SEC-S3000 BioSep-SEC-S4000

Resin Type Silica Silica Silica

Particle Size (µm) 5 5 5

Pore Size (Å) 145 290 500

Efficiency (300 x 7.8 mm) 21,000 19,500 18,000 (minimum number theoretical plates)

Max Temperature (°C) 50* 50* 50*

pH stability 2.5 - 7.5 2.5 - 7.5 2.5 - 7.5


Characterizing and purifying a protein of interest shouldn’t be a complicated, time consuming task. However, when results aren’t reproducible, sample loading is limited, and yields are low, that is exactly what it becomes. BioSep can simplify work and reduce time in the lab with its benefits and advantages outlined below.

“ I have used the BioSep-SEC-S2000 column and have found that it gives good, reproducible profiles of carbohydrate polymers. We use the column as part of a GLP release testing method and require its reproducibility to validate our methods.’’ Anthony Piotrkowski, Gilatech Inc.

Rs-1 is the resolution between IgM and IgA. Rs-2 is the resolution between IgA and IgG. Rs-3 is the resolution between IgG and the void volume. As seen in the figure to the right, the resolution between the peaks of interest remain around 1.5, the ideal resolution value, from 2.0 mg up to 16.0 mg.

Large Loading Capacity

Ensure resolution of critical components regardless of sample loading

Excellent choice for prep or process scale separations

Within protein folding and activity constraints, column capacity can be further increased with the use of small percentages of organic solvent or with an increase in column temperature

2

Reproducibility Guaranteed

Each batch of silica-based material is carefully monitored to ensure that particles have the proper size, shape, and pore characteristics batch-to-batch

Every column is performance tested to ensure the same high quality separation column-to-column

11

Column: BioSep-SEC-S3000 Dimensions: 300 x 7.8 mm Part No.: 00H-2146-K0 Mobile Phase: Potassium phosphate buffer, 20 mM, pH 7.0 Flow Rate: 1 mL/min Detection: UV @ 280 nm Sample: Immunoglobulin (IgM, IgA and IgG) dissolved in the mobile phase. Injected 2 to 16 mg in 100 µL

Loading Capacity of BioSep-SEC-S3000

App ID 15448, 15449, 15450 & 15451

Column: BioSep-SEC-S2000 Dimensions: 300 x 7.8 mm Part No.: 00H-2145-K0 Mobile Phase: 50 mM NaH2PO4

Flow Rate: 1 mL/min Detection: UV @ 220 nm Temperature: 30 °C Sample: 1. Amyloglucosidase 3. Lactalbumin 2. Ovalbumin 4. Aprotinin

Batch-to-Batch & Column-to-Column Reproducibility of BioSep

App ID 5187

Rs-1

Rs-2

Rs-30.0

0.4

0.2

1.2

1.0

0.8

0.6

1.4

1.6

0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0Injection, mg Immunoglobulin/100 µL

Res

olut

ion

Bet

wee

n P

eaks

03

Simplify Research with BioSep™

0 2 4 6 8 10 12 14 min

0

200

400

600

800

1000

mA

U

Batch 1aBatch 2aBatch 1bBatch 2b

1 & 2Denote Different Batches

a & bDenote Different Columns


“ I have been using the BioSep-SEC-3000 column with the BioSep guard column to estimate the percentage of monomeric form of BSA and IgG for more than three years.

Over the last 12 months I critically evaluated the performance of the column using Life Technologies BSA, Sigma amylase. The variation in the retention times of both proteins between runs over the period examined was only 5 %. The mean pressure varied within 10 % between runs. I have enough confidence in the BioSep column that I continue to

use this column for the QC tests in our laboratory. I fully recommend the BioSep-SEC-3000.Minh Lengoc, Life Technologies Ltd., New Zealand ’’

High Sample Yields and Recovery of Biological Activity

Hydrophilically bonded silica gel has low adsorption of proteins resulting in higher yields

Relatively little surface activity due to free silanols limits interaction of protein with base silica

Lysozyme is a somewhat hydrophobic and very basic protein (pKa 11.0) which can be used as a probe to test the surface activity of SEC columns. This figure shows only nominal changes in the value of KD for this enzyme when pH was changed.

3

The KD values were calculated using the formula: KD = (Ve-Vo )/(VT-Vo )

Where Ve is the elution volume of the sample, VT and Vo are the total liquid volume and void volumes of the columns, respectively.

Effect of pH on the Distribution Coefficient KD of Lysozyme

Column: BioSep-SEC-S2000, S3000 and S4000 in series Dimensions: 300 x 7.8 mm (x 3) Part No.: 00H-2145-K0, 00H-2146-K0, and 00H-2147-K0 Mobile Phase: 100 mM PO4 buffer, pH 3.0 - 7.0 Flow Rate: 1 mL/min Detection: UV @ 280 nm Sample: Lysozyme 10 mg/mL 20 µL injected

App ID 5182

Column pH Protein* % Specific Activity* %

BioSep-SEC-S2000 | 3.0 | 85-100 | 86-100 7.0 70-79 95-100BioSep-SEC-S3000 3.0 98-101 96-100 7.0 70-78 96-100BioSep-SEC-S4000 3.0 92-100 95-100 7.0 69-80 94-100*Range for 5 replicate injections

The recovery of Trypsin from BioSep-SEC-S is indicative of the protein yield and activity expected from any analytical or preparative BioSep column.

Recovery of Trypsin from BioSep-SEC-S

Excellent Column Stability

Long column lifetimes due to excellent chemical stability of support materials

Adsorbed materials are easily removed by washing with phosphate buffer at pH 3.0

Strongly retained proteins may be removed by washing with acetonitrile or methanol without compromising performance

4

Column Regeneration Advantage

Easily and completely regenerate by flushing with water overnight

Restore to non-denaturing conditions quickly and easily

5

BioSep™-SEC-S4000

BioSep™-SEC-S3000

BioSep™-SEC-S2000

0.2

0.4

0.6

0.8

1.0

2 3 4 5 6 7 8pH

K D

Simplify Research with BioSep™

04Phenomenex Tel: 310-212-0555 Fax: 310-328-7768 www.phenomenex.com Phenomenex Tel: 310-212-0555 Fax: 310-328-7768 www.phenomenex.com

App ID 5183

Column: BioSep-SEC-S2000 Dimensions: 300 x 7.8 mm Part No.: 00H-2145-K0 Mobile Phase: 50 mM PO4 buffer, pH 6.8 Flow Rate: 1 mL/min Detection: UV @ 215 nm Sample: Proteins + synthetic peptides, 10 µL 1. lgA (300,000) 2. lgG (150,000) 3. Ovalbumin (44,000) 4. Trypsin Inhibitor (20,000) 5. Peptide +5 (3,897) 6. Peptide +4 (3,129) 7. Peptide +3 (2,326) 8. Peptide +2 (1,595) 9. Peptide +1 (827)

Protein and Peptide Size-ExclusionChromatography on BioSep-SEC-S2000

240

220

200

180

160

140

120

100

80

60

40

20

02 4 6 8 10 12 14 min

1

2

3 4

5

6

7

8

9

Separating a protein mixture of molecular weights from 872 to 300,000 daltons illustrates the wide range that can be easily separated on BioSep-SEC-S2000. An excellent linear correlation of 0.994 is obtained for the log MW vs KD plot. Interpolation of unknown protein is easier and more accurate when the separation is efficient and linear.

Exclusion Range in Daltons: Native: 1,000 - 300,000 0.5 % SDS: 200 - 75,000

Broad Molecular Weight Range Separation on BioSep-SEC-S2000

15.6

5.2

4.8

4.4

4.0

3.6

3.2

2.8

1

2

3 4

5 6 78 9

0.0 0.2 0.4 0.6 0.8 1.0KD

Log

MW

I have used BioSep-SEC-2000 from Phenomenex and have found that it provides a robust andeasy to use application for the comparison of

enzymatically treated protein hydrolysates.Tim Emerson, Genencor International

‘‘ ’’

BioSep™-SEC-S Column Options

The BioSep product line offers 3 different phase options to fit the appropriate exclusion range of your sample mixture. All three phases have a narrow pore size distribution, which produces exceptional separations required for high performance gel filtration/size exclusion. Depending on your analysis needs, BioSep 2000, 3000, and 4000 are all available in PEEK hardware and in narrow bore, analyti-cal, and preparative dimensions.

05

BioSep-SEC-S2000

1

2

34

0.0 0.2 0.4 0.6 0.8KD

6.0

5.6

5.2

4.8

4.5

Log

MW

R2= 0.950

BioSep-SEC-S4000

1

2 34

56

7 89

10

13

14

12

0.0 0.2 0.4 0.6 0.8 1.0KD

6.2

5.8

5.4

5.0

4.6

4.2

3.8

3.4

3.0

Log

MW

R2= 0.977

BioSep-SEC-S3000


App ID 5181

SEC of Proteins on BioSep-SEC-S3000

Column: BioSep-SEC-S3000 Dimensions: 600 x 7.8 mm Part No.: 00K-2146-K0 Mobile Phase: 50 mM NaH2PO4 buffer, pH 6.8 Flow Rate: 0.5 mL/min Detection: UV @ 280 nm Sample: Protein mixture, 20 µL injected 1. Thyroglobulin, 670 K 2. IgA, 300 K 3. IgG, 150 K 4. LDH, 143 K 5. Phosphorylase b., 97 K 6. BSA, 68 K 7. Ovalbumin, 44 K 8. Horse radish peroxidase, 40 K 9. Carbonic anhydrase, 30 K 10. Trypsin inhibitor, 20.1 K 11. Myoglobin, 17 K 12. Lysozyme, 14.4 K 13. Insulin, 5.7 K 14. Cyanocobalamin, 1.35 K

Protein Gel Filtration on BioSep-SEC-S4000

Column: BioSep SEC-S4000 Dimensions: 300 x 7.8 mm Part No: 00H-2147-K0 Mobile Phase: 20 mM Sodium Phosphate, pH 7.2 Flow Rate: 0.5 mL/min Temperature: 30 °C Detection: UV @ 280 nm Injection Volume: 20 µL (80 µg total injected) Sample: Protein Mixture 1. Thyroglobulin (670 kDa) 2. Apoferritin (443 kDa) 3. β-Amylase (200 kDa) 4. Bovine Serum Albumin (66 kDa)

5 10 15 20 25 30 35 40 min

2

3

1 12

7

10

App ID 14763

1

2

3

4

120

100

80

60

40

20

0

0 5 10 15 20 25 min

mAU

Proteins on BioSep-SEC-S3000

Mixtures of proteins were subjected to size-exclusion chromatography on BioSep-SEC-S3000. Two mg each of the proteins were dissolved in 0.5 mL of the mobile phase buffer, 50 mM NaH2PO4, pH 6.8. Then 20 µL was injected onto the column. The plot of log MW vs. KD was obtained from runs of three different protein mixtures (r2 = 0.977).

Exclusion Range in Daltons: Native: 5,000 - 700,000 0.5 % SDS: 5,000 - 100,000

2 Large Proteins on BioSep-SEC-S4000

BioSep-SEC-S4000 is capable of resolving proteins of high molecular weight ranging in size from 66,000 to 670,000 daltons. The plot of log MW vs. KD shows an excellent linear relationship with a correlation coefficient of 0.950.

Exclusion Range in Daltons: Native: 15,000 - 2,000,000 0.5 % SDS: 15,000 - 500,000

3

BioSep™-SEC-S Column Options


App ID 5185

Column: BioSep-SEC-S2000 Dimensions: 300 x 7.8 mm Part No.: 00H-2145-K0 Mobile Phase: 50 mM PO4 buffer, pH 6.8 Flow Rate: 1 mL/min Detection: UV @ 215 nm Sample: Natural peptides, 10 µL injected 1. Aprotinin (6,512) 2. Angiotensin (1,296) 3. Enkephalin (555)

App ID 5179

Column: BioSep-SEC-S3000 Dimensions: 300 x 7.8 mm Part No.: 00H-2146-K0 Mobile Phase: 10 mM NaH2PO4 , pH 7.2 Flow Rate: 1 mL/min Detection: UV @ 215 nm Sample: Human serum albumin 0.5 mg/mL, 10 µL injected 1. Aggregate 2. Albumin

Human Serum Albumin

App ID 5186

Column: BioSep-SEC-S3000 Dimensions: 300 x 7.8 mm Part No.: 00H-2146-K0 Mobile Phase: 20 mM PO4 buffer, pH 7.0 Flow Rate: 1.0 mL/min Detection: UV @ 220 nm Temperature: Ambient Sample: 1. Conjugate 2. Porcine Somatotropic Hormone 3. Activated Poly (Gamma Glutamic Acid)

App ID 5188

Column: BioSep-SEC-S3000 Dimensions: 300 x 7.8 mm Part No.: 00H-2146-K0 Mobile Phase: 20 mM PO4 buffer, pH 6.5 with 6 M GnHCI Flow Rate: 1 mL/min Detection: UV @ 280 nm Sample: Protein mixture, 20 µL 1. Phosphorylase b (97,000) 2. BSA (68,000) 3. Ovalbumin (44,000) 4. Carbonic Anhydrase (30,000) 5. Trypsin Inhibitor (20,100) 6. Lysozyme (14,400) 7. 2-mercaptoethanol

Proteins Under Denaturating Conditions

App ID 5178

Column: BioSep-SEC-S3000 Dimensions: 300 x 7.8 mm Part No.: 00H-2146-K0 Mobile Phase: 50 mM PO4 buffer, pH 6.8 Flow Rate: 1 mL/min Detection: UV @ 280 nm Sample: Bio-Rad protein standard, *20 µL injected 1. High MW Aggregates 2. Thyroglobulin 3. IgA 4. IgG 5. Ovalbumin 6. Myoglobin 7. Cyanocobalamin

Protein Standards

App ID 5189

Column: BioSep-SEC-S2000 Dimensions: 300 x 7.8 mm Part No.: 00H-2145-K0 Mobile Phase: 0.2 M Acetate/0.05 M Tris Acetate pH 7.5 Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: Ambient Sample: 1. Asp-45 (10.4) 2. Asp-25 (11.5) 3. Asp-15 (10.9)

Polyaspartic Standards

BioSep™ Applications

Poly (Gamma Glutamic Acid) Conjugated with Porcine Somatotropic Hormone

Natural Peptides

0 4 8 12 16 min

1

23

0 8 16 min

2

1

3

10 15 20 min5

3

2

1

0 2 4 6 8 10 min

1

2

4 6 8 10 12 14 min

62

1 3

4 5

7

07

Chromatogram courtesy of Dr. Joel Swadesh, Alpha Beta Technologies, Boston, MA

Chromatogram courtesy of Mr. Lou DeLuca and Dr. A. P. Wheeler, Department of Biological Sciences, Clemson University, SC.

* Bio-Rad® protein standard was reconstituted according to manufacturer’s instructions.

0 5 10 15 min

12

34

5

6

7


‘‘ My peak shape was greatly improved. Prior to usingBioSep-SEC-S2000 my peak width was greater than 3 minutes for

chondroitin sulfate. With the 2000 my peak width is about 30 seconds.’’ Craig Marshall, IAMS Company

App ID 5180

Column: BioSep-SEC-S3000 Part No.: 00H-2146-K0 Dimensions: 300 x 7.8 mm Mobile Phase: 50 mM NaH2PO4 , pH 6.8 Flow Rate: 1 mL/min Detection: UV @ 280 nm Sample: Bovine immunoglobulins, 8.0 mg/mL, 40 µL injected 1. IgM 2. IgA 3. IgG

Immunoglobulins on BioSep-SEC-S3000

App ID 14995, 14996

Column: BioSep-SEC-S2000 Dimensions: 300 x 7.8 mm Part No.: 00H-2145-K0 Mobile Phase: 50 mM NaH2PO4

Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 25 °C Sample: 1. Lactalbumin aggregate 2. Lactalbumin 3. Salt contaminant 4. Salt contaminant

Desalting of Lactalbumin in 2 M Salt

BioSep™ Applications

BioSep-SEC-S2000 BioSep-SEC-S3000 BioSep-SEC-S4000

Material Hydrophilic bonded silica Hydrophilic bonded silica Hydrophilic bonded silica

Particle Size (µm) 5 5 5

Pore Size (Å) 145 290 500

Exclusion Range in Daltons for Proteins: Native 1,000 - 300,000 5,000 - 700,000 15,000-2,000,000 0.5 % SDS 200 - 75,000 5,000 - 100,000 15,000-500,000 6 M GnHCl 500 - 100,000 1,000 - 150,000 5,000- 700,000

pH Range 2.5 to 7.5 2.5 to 7.5 2.5 to 7.5

Typical Backpressure 450 psi 350 psi 300 psi (for 300 x 7.8 mm column at 1 mL/min)

Maximum 1000 psi 1000 psi 1000 psi Backpressure

Efficiency Minimum plates/meter (using uridine with H2O as mobile phase) (300 x 7.8 mm) 70,000 65,000 60,000

Maximum Flow Rate This is a function of pressure. Columns can withstand up to 1,000 psi, but avoid sudden pressure changes.

Column Hardware Standard: 316 stainless steel column with 2 µm steel frits. Biocompatible columns (PEEK) with 3 µm PEEK frits also available

Maximum Temp. 50 °C for 316 stainless steel; ambient temperature for PEEK

Maximum Salt Conc. 0.5 M

Denaturants 0.5 % SDS, 6 M Guanidine HCl, or 8 M urea

Regeneration After exposure to denaturants, wash immediately with water overnight.

Max. Organic Modifier Up to 100 % CH3CN. Start with 100 % H2O, linear gradient to 100 % CH3CN over 50 min. Up to 90 % CH3OH, 10 % DMSO or 500 mM β-mercaptoethanol.

Cleaning Procedure General protein removal: wash with 30 mL of 0.1 M NaH2PO4, pH 3.0. Hydrophobic protein removal: use acetonitrile gradient. Strongly adsorbed proteins: wash with 30 mL of 0.5% SDS or 6 M Gn thiocyanate or 10 % DMSO.

Storage Overnight storage: run mobile phase at 0.2 mL/minute. Prolonged storage: use 0.05 % NaN3 in H2O or 10 % methanol in H2O.

General Use of a guard column is recommended to prolong column lifetime.

BioSep-SEC-S Technical Data and Specifications

0 2 4 6 8 10 12 min

2

1

3

0 4 8 12 16 min

0

200

mAU

mAU

1

0 4 8 12 16 min

0

2001


Ordering Information

Stainless Steel Columns (mm): Narrow Bore Analytical Preparative 300 x 4.6 300 x 7.8 600 x 7.8 300 x 21.2 600 x 21.2Phases2000 I 00H-2145-E0 00H-2145-K0 I 00K-2145-K0 00H-2145-P0 I 00K-2145-P03000 00H-2146-E0 00H-2146-K0 00K-2146-K0 00H-2146-P0 00K-2146-P04000 00H-2147-E0 00H-2147-K0 00K-2147-K0 00H-2147-P0 00K-2147-P0

SecurityGuard™ Cartridges require universal holder Order No.: KJO-4282

for ID: 3.2-8.0 mm

PEEK (Biocompatible) Columns (mm): PEEK (Biocompatible) Guards (mm): Narrow Bore Analytical Narrow Bore Analytical 300 x 4.6 300 x 7.5 30 x 4.6 75 x 7.5Phases Phases2000 I 00H-2145-E0-BV 00H-2145-J0-BV 2000 03A-2145-E0-BV 03C-2145-J0-BV3000 00H-2146-E0-BV 00H-2146-J0-BV 3000 03A-2146-E0-BV 03C-2146-J0-BV4000 00H-2147-E0-BV 00H-2147-J0-BV 4000 03A-2147-E0-BV 03C-2147-J0-BV

Other column dimensions available upon request.

Stainless Steel Guards (mm): SecurityGuard Narrow Bore Express Analytical Preparative Cartridges 30 x 4.6 35 x 7.8 75 x 7.8 60 x 21.2 4 x 3.0mmPhases 2000 I 03A-2145-E0 03Q-2145-K0 03C-2145-K0 03R-2145-P0 AJ0-44873000 03A-2146-E0 03Q-2146-K0 03C-2146-K0 03R-2146-P0 AJ0-44884000 03A-2147-E0 03Q-2147-K0 03C-2147-K0 03R-2147-P0 AJ0-4489

FREE GUIDE to Protein and Peptide Analysis by HPLC

Call your Phenomenex representativefor your FREE copy!

Theory and application of protein and peptide separation techniques

Practical guidelines for selecting columns, developing and optimizing separations

Useful tips and traps on column care, cleaning and storage

Review of Micro LC in Biochromatography

BioSep, Phenex, and SecurityGuard are trademarks of Phenomenex, Inc. Bio-Sil is a registered trademark of Bio-Rad. Zorbax GF is a registered trademark of Aglient Technologies. Puradisc is a trademark of Whatman. Phenomenex is in no way affiliated with the above companies. Columns used for comparison were purchased directly from the original manufacturer. Comparisons shown may not be representative of every application. Information, descriptions, and specifications in this publication are subject to change without notice.

© 2007 Phenomenex, Inc. All rights reserved.

Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions

App ID 14752

Aqueous SEC 1 Column Check Standard

Part No.: ALO-3042 Price:

(For BioSep SEC-S and other protein SEC columns) Unit quantity: Dry; Reconstituted to 2 mL Contains: Bovine thyroglobulin; Human gamma globulin; Ovalbumin; Myoglobin; Uridine (reconstitute with 1 mL of 100 mM Phosphate buffer pH=6.8) Diluent: 100 mM Sodium phosphate buffer, pH 6.8 Storage: Add 0.1 % NaN3 to the solution and refrigerate

Test Conditions Mobile phase: 100 mM Sodium phosphate buffer, pH 6.8 Flow rate: 1.0 mL/min for a 300 x 7.8 mm column Injection volume: 10 µL Detection: UV @ 280 nm

BioSep™ Ordering Information

If BioSep™ does not provide you with equal or better separations than any other GFC column of similar porosity, type and comparable dimensions, send in your comparative data within 45 days and keep the BioSep™

for FREE.

09

AA0-3908

$1,475 $1,550 $390 $425

$320 $335 $375 $725 $299/10 pk

$1,330 $1,150 $1,880 $3,250 $4,700

$ 119


Phenex Features

Phenex™ Syringe Filters & MembranesFor Sample and Solvent Filtration Prior to Chromatography!

Phenex Syringe Filters Rapid filtration of HPLC and GC

samples prior to analysis

Particulate, PVC, and extractable-free filters

Consistent, reliable performance

10

Ordering InformationOrdering Information

Phenex Syringe Filters

Tip: Try a Sample Pack!The best way to determine if a specific Phenex membrane is suitable for your application.Request yours today by phone or visit www.phenomenex.com/sample

1. 17 mm diameter.2. Glass fiber filters are 26 mm diameter and made of borosilicate.

They will remove 90 % of all particles >1.2 µm. 3. Housing material is methacrylate butadiene styrene (MBS)

polymerisate. Also known as Cryolite.4. Cellulose acetate is surfactant-free.5. 26 mm diameter.6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting

with IPA.7. Additional dimensions and membrane types are available. Please

contact your local Phenomenex technical consultant or distributor for availability or assistance.

8. Larger quantity purchases at significant savings are available.

Low absorption Low hold-up volumeParticulate-free Certified qualityMaximum chemical compatibility 100 % integrity testedMinimum extractables Easy pore identificationExcellent flow rate PVC-freeHigh total throughput Bi-directional use


If Phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, send in your comparative data within 45 days and keep the Phenex products for FREE!

4 mm Diameter 15 mm Diameter 25 mm Diameter for < 2 mL sample volumes for 2 - 10 mL sample volumes for 10 - 100 mL sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price 0.45 µm Phenex-RC 3 AF0-3103-12 100/Pk $135 AF0-2103-12 100/Pk $155 AF0-8103-12 5 100/Pk $195 (Regenerated Cellulose) AF0-3103-52 500/Pk 540 AF0-2103-52 500/Pk 620 AF0-8103-52 5 500/Pk 780 Phenex-PES 3 — — — AF2-5108-12 1 100/Pk 180 AF0-8108-12 5 100/Pk 215 (Polyethersulfone) — — — — — — AF0-8108-52 5 500/Pk 860 Phenex-PTFE 6 AF0-3102-12 100/Pk 145 AF0-2102-12 100/Pk 180 AF0-1102-12 100/Pk 215 (Polytetrafluoroethylene) AF0-3102-52 500/Pk 580 AF0-2102-52 500/Pk 720 AF0-1102-52 500/Pk 860 Phenex-NY AF3-3107-12 100/Pk 140 AF2-5107-12 1 100/Pk 160 AF0-1107-12 100/Pk 205 (Nylon) AF3-3107-52 500/Pk 560 AF2-5107-52 1 500/Pk 640 AF0-1107-52 500/Pk 820 Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8B09-12 100/Pk 245 (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8B09-52 500/Pk 980 0.20 µm Phenex-RC 3 AF0-3203-12 100/Pk $135 AF0-2203-12 100/Pk $155 AF0-8203-12 5 100/Pk $195 (Regenerated Cellulose) AF0-3203-52 500/Pk 540 AF0-2203-52 500/Pk 620 AF0-8203-52 5 500/Pk 780 Phenex-PES 3 — — — — — — AF0-8208-12 5 100/Pk 215 (Polyethersulfone) — — — — — — AF0-8208-52 5 500/Pk 860 Phenex-PTFE 6 AF0-3202-12 100/Pk 145 AF0-2202-12 100/Pk 180 AF0-1202-12 100/Pk 215 (Polytetrafluoroethylene) AF0-3202-52 500/Pk 580 AF0-2202-52 500/Pk 720 AF0-1202-52 500/Pk 860 Phenex-NY AF3-3207-12 100/Pk 140 AF2-5207-12 1 100/Pk 160 AF0-1207-12 100/Pk 205 (Nylon) AF3-3207-52 500/Pk 560 AF2-5207-52 1 500/Pk 640 AF0-1207-52 500/Pk 820 Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8A09-12 100/Pk 245 (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8A09-52 500/Pk 980 1.20 µm Phenex-GF 2 Prefiltration of heavily contaminated or highly viscous samples. When used in-line preceding AF0-8505-12 100/Pk $150 (Glass Fiber) a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. AF0-8505-52 500/Pk 600

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PRSRT STDU.S. POSTAGEPAIDPERMIT #185TORRANCE, CA

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(800) 541-HPLC(310) [email protected]

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USA411 Madrid Ave.Torrance, CA90501-1430USA

(310) 212-0555(310) [email protected]

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department by telephone, fax or email: [email protected].

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