a biology and engineering cooperative project james w. stevens m. karen newell university of...
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A Biology and A Biology and Engineering Engineering
Cooperative ProjectCooperative ProjectJames W. StevensJames W. StevensM. Karen NewellM. Karen Newell
University of Colorado at Colorado University of Colorado at Colorado SpringsSprings
30 April 200530 April 2005
general backgroundgeneral background
connection between cellular connection between cellular metabolic pathways and immune metabolic pathways and immune system responsesystem response
surface signals tied to mitochondrial surface signals tied to mitochondrial activity and fuelactivity and fuel
cooperative effortcooperative effort
biologybiology– science driverscience driver– cell culturescell cultures
growinggrowing conditionsconditions
– cell counting and cell counting and physical physical preparationpreparation
engineeringengineering– data acquisitiondata acquisition– temperature temperature
measurementsmeasurements– data reductiondata reduction– data smoothingdata smoothing
background-this projectbackground-this project
2 mouse leukemia lines2 mouse leukemia linesL1210 – not drug resistantL1210 – not drug resistant
L1210/ddp– drug resistantL1210/ddp– drug resistant cultured to 10^6 to 10^8 cellscultured to 10^6 to 10^8 cells stressed with bleachstressed with bleach
– measurable, repeatable differencesmeasurable, repeatable differences– different culture conditions, metabolic different culture conditions, metabolic
statesstates
cumulative heat release
-4
-2
0
2
4
6
8
10
12
14
16:51:36 16:52:19 16:53:02 16:53:46 16:54:29 16:55:12 16:55:55
time
jou
les
L1210ddp L1210
dt
dEWQG
)( TThAdt
dTmcG
data analysisdata analysis
first law of thermodynamics:first law of thermodynamics:
measured heat generation rate
-2
-1
0
1
2
3
4
5
6
16:52:05 16:52:10 16:52:14 16:52:18 16:52:23 16:52:27 16:52:31
time
wat
ts
L1210ddp L1210
0 0.2 0.4 0.6 0.8 10
0.5
1
*
*
*)(dt
bt
ec
aety
data smoothingdata smoothing
least square curve fit:least square curve fit:
minimize minimize
2ˆ*)( iyty
measured heat generation rate
-2
-1
0
1
2
3
4
5
6
16:52:05 16:52:10 16:52:14 16:52:18 16:52:23 16:52:27 16:52:31
time
wat
ts
L1210ddp, smoothed L1210, smoothed L1210ddp L1210
summarysummary
leverage complementary capacities– leverage complementary capacities– specific to people and projectspecific to people and project
involve studentsinvolve students
plunge into a new areaplunge into a new area
other cooperative efforts at UCCSother cooperative efforts at UCCS
biomechanics –biology, mechanical biomechanics –biology, mechanical engineeringengineering
orthopedic mechanics –MERIC, orthopedic mechanics –MERIC, biology, mechanical engineeringbiology, mechanical engineering
feedback controls –MAE, biologyfeedback controls –MAE, biology microgravity – MAEmicrogravity – MAE assistive technologies – MATH, assistive technologies – MATH,
biologybiology