| INVESTIGATION

A Gene for Genetic Background in Zea mays:Fine-Mapping enhancer of teosinte branched1.2 to a

YABBY Class Transcription FactorChin Jian Yang,* Lisa E. Kursel,*,1 Anthony J. Studer,*,2 Madelaine E. Bartlett,†,3 Clinton J. Whipple,†

and John F. Doebley*,4

*Laboratory of Genetics, University of Wisconsin–Madison, Wisconsin 53706 and †Department of Biology, Brigham YoungUniversity, Provo, Utah 84602

ABSTRACT The effects of an allelic substitution at a gene often depend critically on genetic background, i.e., the genotypes at othergenes in the genome. During the domestication of maize from its wild ancestor (teosinte), an allelic substitution at teosinte branched(tb1) caused changes in both plant and ear architecture. The effects of tb1 on phenotype were shown to depend on multiplebackground loci, including one called enhancer of tb1.2 (etb1.2). We mapped etb1.2 to a YABBY class transcription factor (ZmYAB2.1)and showed that the maize alleles of ZmYAB2.1 are either expressed at a lower level than teosinte alleles or disrupted by insertions inthe sequences. tb1 and etb1.2 interact epistatically to control the length of internodes within the maize ear, which affects how denselythe kernels are packed on the ear. The interaction effect is also observed at the level of gene expression, with tb1 acting as a repressorof ZmYAB2.1 expression. Curiously, ZmYAB2.1 was previously identified as a candidate gene for another domestication trait in maize,nonshattering ears. Consistent with this proposed role, ZmYAB2.1 is expressed in a narrow band of cells in immature ears that appearsto represent a vestigial abscission (shattering) zone. Expression in this band of cells may also underlie the effect on internodeelongation. The identification of ZmYAB2.1 as a background factor interacting with tb1 is a first step toward a gene-level under-standing of how tb1 and the background within which it works evolved in concert during maize domestication.

KEYWORDS domestication; epistasis; genetic-background; maize; teosinte-branched1

THE effects of an allelic substitution at a gene can varywidely depending on the genetic background in which its

effects are assayed (Chandler et al. 2013). In extreme cases,the effect of an allele may even exhibit a change in direction-ality across different genetic backgrounds (Wade et al. 2001).Often, genetic background is considered a “nuisance factor”and thus something that needs to be controlled by the usageof inbred lines in experiments involving model organisms

such as maize, Arabidopsis, and mice, because the effect ofan allele can change under different genetic backgrounds(Wade 2002; Chandler et al. 2013). However, in evolutionarybiology, a knowledge of the extent and dynamics of geneticbackground is essential for a complete understanding of theevolutionary process.

The teosinte branched (tb1) gene of maize (Zea mays ssp.mays) has been identified as one of the genes involved inthe domestication of maize from its wild progenitor, teosinte(Z. mays ssp. parviglumis). tb1 acts pleiotropically to regulateboth the architecture of the plant overall and the structure of thefemale inflorescence or ear (Doebley et al.1997). Plants carryingthe maize allele of tb1 have fewer, shorter branches than plantscarrying teosinte alleles. The ears of plants with the maize allelehave short internodes, such that the kernels are more tightlypacked together as compared to plants with teosinte alleles.Substitution of a teosinte allele for a maize allele of tb1 can alsopartially transform the ear (female) into a tassel (male).

The effects of the maize vs. teosinte alleles of tb1 onboth plant and ear traits vary with genetic background, and

Copyright © 2016 by the Genetics Society of Americadoi: 10.1534/genetics.116.194928Manuscript received August 17, 2016; accepted for publication September 28, 2016;published Early Online October 10, 2016.Supplemental material is available online at www.genetics.org/lookup/suppl/doi:10.1534/genetics.116.194928/-/DC1.1Present address: Molecular and Cellular Biology Graduate Program, University ofWashington, Seattle, WA 98195.

2Present address: Department of Crop Sciences, University of Illinois Urbana-Champaign, Urbana, IL 61801.

3Present address: Department of Biology, University of Massachusetts-Amherst,Amherst, MA 01003.

4Corresponding author: Laboratory of Genetics, 425 Henry Mall, University ofWisconsin-Madison, WI 53706. E-mail: jdoebley@wisc.edu

Genetics, Vol. 204, 1573–1585 December 2016 1573

epistatic interactions between tb1 and other loci that contrib-ute to the background effects have been described (Doebleyet al. 1995; Studer and Doebley 2011). Among these otherloci is a quantitative trait locus (QTL) called enhancer of tb1.2(etb1.2). The principal effect of etb1.2 is on the length ofinternodes within the ear, although it also affects the sex ofthe ear itself, whether partially converted into a tassel by theteosinte allele. Interestingly, etb1.2 is located only 6 cM up-stream of tb1.

Here, we report the fine-mapping of etb1.2 to a YABBYfamily transcriptional regulator called ZmYAB2.1. Expressionassays show that somemaize alleles of etb1.2 are expressed ata lower level than teosinte alleles, while other maize allelesare loss-of-function with disrupted coding sequences. Thereis an epistatic interaction between etb1.2 and tb1 such thatthe effects of the maize vs. teosinte allele of etb1.2 are muchstronger when tb1 is fixed for the teosinte allele. This inter-action effect is seen both at the level of phenotype andZmYAB2.1 expression. tb1 functions as an upstream regulator(repressor) of etb1.2. Surprisingly, ZmYAB2.1 has beenpreviously implicated as a gene controlling another domesti-cation trait, nonshattering inflorescences (Lin et al. 2012).Consistent with an effect of ZmYAB2.1 on shattering, weshow that ZmYAB2.1 is expressed in a narrow band of cellsthat likely represents a vestigial shattering (abscission) zone.Expression in this band of cells may also explain the effect onear internode length. This report provides the first gene-levelview of how genetic background influences the effects of tb1.

Materials and Methods

Fine-mapping of etb1.2

We constructed 45 recombinant chromosome nearly isogeniclines (RC-NILs) carrying different teosinte (Z. mays ssp. par-viglumis; Iltis and Cochrane collection 81) introgressionswithin the interval of etb1.2 QTL (Studer and Doebley2011) for fine-mapping. We obtained these RC-NILs by firstcrossing two nearly isogenic recombinant inbred lines (NI-RILs)with the teosinte allele at tb1 andmaizeW22 background butdifferent alleles (maize vs. teosinte) at etb1.2 (SupplementalMaterial, Figure S1). We then screened 1425 plants from theresulting F2 population for recombinants in the etb1.2 inter-val using two flanking markers: umc2047 and umc1411.Plants with recombinant chromosomes were then selfed toproduce the 45 RC-NILs. We further narrowed the recom-bination breakpoints of each RC-NIL using genotype-by-sequencing (GBS) markers (Elshire et al. 2011), Sangersequencing-based SNP markers, and insertion/deletion-basedmarkers (Table S1).

We planted all 45 RC-NILs and the two NI-RIL parentsusing a randomized complete block design at the Universityof Wisconsin West Madison Agricultural Research Station(UW-WMARS) in summer 2012. These lines were planted insix blocks with 68 plots in each block, where 24 RC-NILs hadone seed-lot planted and 21 other RC-NILs had two seed-lotsplanted. Each plot was 3.6 m long and 0.9 m wide, and

was sowed with 12 seeds. For each plot, we harvested thetop ear from five plants for scoring the ear internode length(length of 4–10 consecutive kernels divided by the number ofkernels in mm). This trait was also known as average lengthof the cupules in the ear (CUPL) or 10-kernel length (10 KL)in previous publications (Doebley et al. 1995; Studer andDoebley 2011).

We computed the least square means (LSMs) of earinternode length to be used in fine-mapping. These LSMswere calculated using the MIXED procedures in SAS (Littellet al. 1998), and the model is shown as follows:

Yijk ¼ mþ aiðbjÞ þ bj þ ck þ eijk

where Yijk is the ear internode length for an introgressionline, m is the overall mean, ai is the fixed seed-lot effect, bjis the fixed line effect, ck is the random block effect, and eijk isthe experimental error.

To fine-map the interval within which the etb1.2 QTL islocated, we looked for correspondence between the intro-gressed teosinte chromosomal segments and LSMs of the45 RC-NILs. We first matched the introgressed segments ofthe 45 RC-NILs to their corresponding LSMs. Next, we sortedthe 45 RC-NILs by their LSMs and identified two distinctclasses of LSMs separated by nonoverlapping error bars.Under the assumption that there is a single gene contributingto the QTL, one expects a clean segregation of the lines intotwo groups based on their LSMs, such that there is a smallchromosomal interval for which all lines in one group haveteosinte genotype and all lines in the other group have maizegenotype. The interval thus identified is the causative inter-val that contains the QTL. Genotype and phenotype data areavailable from the Dryad Digital Repository: (http://dx.doi.org/10.5061/dryad.n5j54/1).

Gene characterization

Fine-mapping identified ZmYAB2.1 as the only gene withinthe refinedQTL interval.We obtained the genomic sequencesof ZmYAB2.1 for B73, W22, and our teosinte parent bySanger sequencing. Resequencing the B73 allele was neces-sary because the available published sequences were incom-plete. We designed primers for sequencing ZmYAB2.1 basedon the GRMZM2G085873 gene model in the maize referencegenome AGPv2 (http://www.maizegdb.org) and an im-proved ZmYAB2.1 sequence assembly that was published asZmSh1-1 (Lin et al. 2012). A complete list of primers used forsequencing can be found in Table S2. A B73 BAC clone(ZMMBBc0564C07) from the Arizona Genome Institutewas also used for facilitating the sequencing work. We per-formed all sequence alignment using Sequencher version 5.1(Gene Codes Corporation). All our sequences have beendeposited in GenBank (Accession Numbers: KX687024–KX687134).

We performed phylogenetic analysis of the YABBY genefamily acrossdiverseplant species using107publishedYABBYpeptide sequences. Of these 107 YABBY peptide sequences,

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101 were taken from Yamada et al. (2011) and six otherswere taken from UniProt (The UniProt Consortium 2015)and Lin et al. (2012). We aligned all 107 YABBY peptidesequences using Clustal X2.1 (Larkin et al. 2007) and Se-Alv2.0a11 (Rambaut 2002). Conserved positions of the107 YABBY peptide sequences were extracted using Gblocks0.91b (Castresana 2000). We performed Bayesian phyloge-netic analysis of the extracted YABBY peptide sequencesusing MrBayes v3.1.2 on XSEDE provided by the CIPRESScience Gateway (Miller et al. 2010). Parameters used in thisphylogenetic analysis were kept the same as the parametersoriginally used by Yamada et al. (2011). Themodel for among-site rate variation (rates=) was set to invariant + gamma andrate matrix for amino acids (aamodelpr=) was set to Jones(Jones et al. 1992). Trees were sampled at every 1000 gener-ations (sampfreq=) for 5,000,000 generations (ngen=), withthe first 25% discarded as burn-in. We then verified the con-vergence of theMarkov ChainMonte Carlo (mcmc) runs usingTracer v1.60 (Rambaut et al. 2014).

Population genetics

We investigated for evidence of selection during domestica-tion around ZmYAB2.1 using the Hudson–Kreitman–Aguade(HKA) test (Hudson et al. 1987), Tajima’s D (Tajima 1989),and coalescent simulation (Innan and Kim 2004). To performthese analyses, we first sequenced the 59 end of ZmYAB2.1(spanning the 59-UTR and exon 1) and two regions upstreamof ZmYAB2.1 from 19 maize landrace accessions, 16 teosinteaccessions, and 1 Z. diploperennis accession (outgroup forHKA test) (Table S3). These three regions were chosen tocover the fine-mapped etb1.2 interval. We also included se-quences from six previously identified unlinked neutral genes(AY104395, AY106816, AY107192, AY107248, AY111546,and AY111711) (Zhao et al. 2011) as control genes forthe HKA test. All sequence alignments were done usingSequencher version 5.1 (Gene Codes Corporation). We ana-lyzed the sequence alignments for nucleotide (nt) diversity(p), HKA test (Hudson et al. 1987), and Tajima’s D (Tajima1989) using DnaSP v5.10.01 (Librado and Rozas 2009). Per-cent loss in diversity was calculated as ð12pm=ptÞ3 100:We performed coalescent simulation using software fromInnan and Kim (2004) with the same parameters used byZhao et al. (2011).

We investigated the distribution of four null ZmYAB2.1alleles in 69 Z. mays ssp. mexicana accessions, 73 Z. maysssp. parviglumis accessions, 127 Z. mays ssp.mays accessions(landrace), 18 teosinte inbred lines, and 58 maize inbredlines (Table S4). The four null ZmYAB2.1 alleles were discov-ered during our diversity sequencing and expression analyseson ZmYAB2.1 in multiple maize inbred lines. We namedthese null alleles after the maize inbred line in which theywere initially identified and the corresponding mutation,namely B73_Spm, W22_Spm, Mo17_82bp, and Ki3_2bp.We screened for the B73_Spm and W22_Spm alleles usingPCR with a three primers mix (Table S5) followed by agarosegel electrophoresis. For each null allele screen, we designed

two primers to flank the insertion and a third primer that sitswithin the insertion. We scored the PCR products based onsize differences on a 4% agarose gel. We screened for theMo17_82bp and Ki3_2bp alleles using PCR with fluorescentprimers (Table S5) followed by assaying the PCR products inan ABI 3730 DNA Analyzer (Applied Biosystems, Foster City,CA). We then analyzed the results using GeneMarker version1.7 (Softgenetics, State College, PA). To sum up all the nullalleles, we plotted the geographical distributions of allassayed alleles on a map using Google Map Engine Lite(https://mapsengine.google.com/map/) (Figure S2).

Gene expression

Quantitative RT-PCR (qPCR): We performed gene expres-sion assays for ZmYAB2.1 using RNA extracted from imma-ture ears between 10 and 30 mm (roughly 60 days postplanting) using the standard Trizol (Invitrogen, Carlsbad,CA) protocol. We evaluated the isolated RNA quality usingagarose gels made from 3-(N-morpholino)propanesulfonicacid (MOPS) buffer and formaldehyde. Following that, wetreated each RNA samplewith DNase prior to complementaryDNA (cDNA) synthesis using standard oligo dT primers andSuperscript III reverse transcriptase (Invitrogen). We also ver-ified the cDNA quality and absence of DNA contamination byPCR (Taq Core Kit, QIAGEN, Valencia, CA) by amplifyingacross two exons using flanking primers in GRMZM2G126010(actin) (Table S5).

We performed qPCR analysis of ZmYAB2.1 in the two pa-rental NI-RILs (T1L_C07 and T1L_D17), carrying either theteosinte introgression or maize (W22) allele at etb1.2. Imma-ture ears for these two NI-RILs were harvested from plantsgrown at UW-WMARS in summer 2013. We specificallydesigned the PCR primers to span across two or more exonsas an insurance against DNA contamination during qPCR.Weused two sets of primers to amplify a 220-bp fragment fromZmYAB2.1 and a 201-bp fragment from GRMZM2G126010(actin gene), respectively (Table S5). We normalized ZmYAB2.1expression level based on GRMZM2G126010 (actin gene). AllqPCR reactionswere done using Power SYBRGreen PCRMasterMix (Applied Biosystems) in an ABI Prism 7000 Sequence De-tection System (Applied Biosystems). The following steps wereused in the qPCR reactions: activation at 95� for 10 min, de-naturation at 95� for 15 sec, annealing and extension at 60�for 1 min, and repeated for 40 cycles. A total of 20 biologicalreplicates and three technical replicates were used for eachNI-RIL in this expression analysis.

Similarly, we also performed qPCR analysis of ZmYAB2.1with two RC-NILs: IN1207 carries the teosinte allele ofZmYAB2.1, and IN0949 carries a recombinant allele withmaize sequence in the 59 regulatory region and teosinte se-quence in the coding region of ZmYAB2.1 (Figure S3). Imma-ture ears for these two RC-NILs were harvested from plantsgrown at the University of Wisconsin Walnut Street Green-house (UW-WSGH) in winter 2013.We used the same primersets, reagents, instruments, and replicate setup as describedin the previous paragraph.

etb1.2, YABBY Class Transcription Factor 1575

Allele-specific expression assays: We also investigated thedifference in the mRNA abundance of ZmYAB2.1 betweenmaize and teosinte alleles using an allele-specific expressionassay. This assays compares mRNA abundance of the twoalleles when heterozygous in a plant. The maize ZmYAB2.1allele was represented by two maize inbreds (A619 andOh43) and two maize landrace inbreds (MR12 and MR19),while the teosinte ZmYAB2.1 allele was represented by twoteosinte inbreds (TIL01 and TIL11) (Table S4). These lineswere chosen because all of them have functional allelesencoding a full-length protein (Table S4). RNA was extract-ed from immature inflorescences at the tip of the lateralbranch from the F1 hybrid plants grown at the UW-WSGHin winter 2014.

For each F1 RNA sample, cDNA was synthesized usingthe method described above. In contrast to qPCR, the allele-specific expression analysis requires small insertion/deletion(indel) that distinguishes the two alleles being compared.As a consequence, we designed a set of primers (Table S5)flanking an indel for amplifying a small fragment within the59-UTR of ZmYAB2.1 (Table S6). To perform the allele-specific expression analysis, we ran a PCR of the 59-UTR frag-ment with fluorescent primers on the cDNA, and assayed thePCR products in an ABI 3730 DNA Analyzer (Applied Biosys-tems). We obtained the areas under the peaks correspondingto maize and teosinte alleles using Gene Marker version 1.7(Softgenetics). We calculated the relative expression ofthe alleles by taking the ratio of areas under the peaks forteosinte allele over maize allele. For each F1 hybrid, therewere between three to eight biological replicates and threetechnical replicates. Of the eight possible teosinte 3 maizeF1s, only six had different allele sizes for the indel.

Since different alleles may have different efficiencies forPCR amplification, we needed to perform correction on theallele-specific expression assay using DNA controls. We usedDNA isolated from the F1 plants as a control with a knownratio of 50:50 for the teosinte and maize alleles. DNA wasisolated from leaf tissue of each plant using a standard CTABextraction protocol (Rogers and Bendich 1985). We includedthese DNA controls in each technical replicate of the assay.We identified any PCR amplification bias by taking the ratioof areas under peaks for maize allele over teosinte allele,which is also known as the correction factor. We then multi-plied the relative expression by the correction factor to obtainthe final corrected relative expression of teosinte allele overmaize allele. For example, if the ratio of teosinte expressionlevel over maize expression level is 2.0, and the correctionfactor is 1.5, then the final corrected relative expressionwould be 3.0 to account for the PCR amplification bias to-ward the maize allele. Gene expression data are availablefrom the Dryad Digital Repository: (http://dx.doi.org/10.5061/dryad.n5j54/3).

Interactions with tb1

To investigate any interaction between ZmYAB2.1 and tb1 inthe regulation of ear internode length, we selected 20NI-RILs

for phenotypic and expression analyses (Figure S4). For thephenotypic analysis, we planted these 20 NI-RILs using a ran-domized complete block design at UW-WMARS in summer2014. These lines were planted in six blocks with 20 plots ineach block. Each plotwas 3.0m long, 0.9mwide, and sownwith10 kernels. For each plot, we harvested the uppermost ear fromfive plants for scoring the ear internode length (length in mm of4–10 consecutive kernels divided by the number of kernels). Weobtained the LSMs for the ear internode length using a similarmodel to that described above. For the expression analysis, wequantified both ZmYAB2.1 and tb1 expression levels in four im-mature ears from each of these 20 NI-RILs. The protocol for theexpression analysis was the same as described above.

We tested for three different interactions between ZmYAB2.1and tb1 genotypes on ZmYAB2.1 expression, tb1 expression, andear internode length. We tested for these interactions using thefollowing linear model implemented in R (R Core Team 2016):

Yijk ¼ mþ ai þ bj þ abij þ eijk

where m is the overall mean, ai is ZmYAB2.1 genotype maineffect, bj is tb1 genotype main effect, abij is interaction effectbetween ZmYAB2.1 and tb1, and eijk is the experimental error.Three different data sets (ZmYAB2.1 expression, tb1 expres-sion, and ear internode length) were used for Yijk: Phenotypeand gene expression data are available from the Dryad DigitalRepository (http://dx.doi.org/10.5061/dryad.n5j54/2).

In situ hybridization

Maize ears (20–30 mm) and teosinte ears (10–20 mm)were collected and fixed in 4% para-formaldehyde, then em-bedded in paraffin, as described by Jackson (1991). Twodigoxygenin-UTP-labeled RNA antisense probes were createdusing a MEGAScript T7 kit (Ambion). One probe to the 39-UTR was amplified with YABBY39UTRF1 (59-CCTATGAAAGTAGCAACAAG-39) and YABBY39UTRR1 (59-ATACACGACACTCACAAACTA-39). Another probe containing coding se-quence from exons 2 and 3 downstream of an enhancer/suppressor mutator insertion was originally amplified fromcDNA with the primers YABBYExon2.3F1 (59-CGTCCAGAATCACTACTCAC-39) and YABBYExon2.3R1 (59-GTGCAGCATATGATCCAGAT-39). A third probe to the 59-UTR was ampli-fiedwith YABBY 59UTRF1 (59-CTGGATGGCCCTCTGATTAG-39)and YABBY59UTRR1 (59-ATCTGGGCCGCCGACGACAT-39). The39-UTR probe was mixed 1:1 with either the exon 2/3 probe orthe 59 UTR probe for hybridizations in Figure 4, A–D, while onlythe 39-UTR probe was used in Figure 4, E and F and Figure S6.Sectioning, hybridization, washing, and detections was per-formed as previously described (Jackson 1991; Jackson et al.1994), except that hybridization and washing temperaturewas raised from the standard 55–65� for the 39-UTR probe.

Data availability

The authors state that all data necessary for confirming theconclusions presented in the article are represented fullywithin the article.

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Results

Fine-mapping of etb1.2

We successfully narrowed etb1.2 down to a 68-kb interval(AGPv2, chr1: 260,383,398–260,448,142) containing exon1 and 59 regulatory region of the maize filtered-gene-setmember GRMZM2G085873. Among the 45 RC-NILS andtwo parental NI-RILs, we observed a segregation of the linesinto two distinct classes for the LSMs of ear internode length(Figure 1). Examination of the genotypes of these two classesreveals that the class with short internodes carries maize ge-notype between markers SBM03 and SBM04, and the classwith long internodes carries teosinte genotype between thesemarkers (Figure 1). Interestingly, while this interval includesexon 1 of GRMZM2G085873, the only coding sequencedifference between the W22 and teosinte alleles is a singlesynonymous substitution in exon 1, which seems unlikely toconfer differences in ear internode length betweenmaize andteosinte. Based on these observations, we hypothesize that acis-regulatory change upstream of GRMZM2G085873 is thelikely causal polymorphism underlying etb1.2.

Gene characterization

Examination of the annotation for GRMZM2G085873revealed that it encodesaproteinwithhomology to theYABBYtranscription factor family, first identified in Arabidopsis.Based on the maize B73 AGPv2 reference genome,GRMZM2G085873 (260,364,371–260,367,349) possessesfive exons; however, the Arabidopsis YABBY homolog con-tains six exons. Further examination of the B73 AGPv2reference sequence shows that the assembly has a contig inthe wrong orientation (Figure S6). We resequenced this re-gion in B73, and our sequencing results indicate thatGRMZM2G085873 actually has six exons in B73, like itsArabidopsis counterpart (Figure S7). Our sequence data alsoshow that W22 and our teosinte allele possess six exons. Inour complete alignment of 10,677 bp, the B73 sequence is10,046 bp, the W22 sequence is 8993 bp, and the teosintesequence is 8362 bp. A BLAST search of the complete se-quence using RepeatMasker (Smit et al. 2015) on RepBase(Jurka et al. 2005) identified a defective enhancer/suppressormutator element, also known as En/Spm-I or dSpm (Peterson1953; McClintock 1954), inserted between the first and sec-ond exons in B73. We also found a similar, but not identical,insertion in W22 at a different position between the first andsecond exons (Figure S7).

Phylogenetic analysis revealed that GRMZM2G085873belongs to the YAB2 clade of the five known clades in theYABBY gene family (Figure S8) (Siegfried et al. 1999;Bowman 2000; Yamada et al. 2011). Given this result, wedesignated GRMZM2G085873 as ZmYAB2.1. YABBY genesencode transcription factors, which consist of a C2C2 zincfinger domain that binds DNA and a helix-loop-helix YABBYdomain that recognizes DNA-binding sites (Bowman 2000).YABBY transcription factors are mainly expressed in theabaxial domain of lateral organs and contribute to adaxial–

abaxial polarity (Siegfried et al. 1999), although in maize thepolarity is switched and they are expressed adaxially (Juarezet al. 2004). Vegetatively-expressed YABBY genes (FIL, YAB2,YAB3, and YAB5) were initially thought to directly specifyleaf adaxial–abaxial polarity. However, recent analysis indi-cates that YABBY genes play a central role in diverse leafgrowth and patterning, including blade expansion, inhibitionof meristematic genes, and signaling to the adjacent meri-stem (Goldshmidt et al. 2008; Sarojam et al. 2010).

ZmYAB2.1 (also called ZmSh1-1) was previously reportedas the ortholog of Shattering1 of sorghum, a key gene con-trolling the difference between shattering and nonshatteringinflorescences in wild vs. domesticated sorghum (Lin et al.2012). In maize-teosinte QTL studies, a QTL for inflorescenceshattering mapped to ZmYAB2.1, making it a strong candidatefor the gene controlling the difference in shattering betweenteosinte and maize as well (Lin et al. 2012). In teosinte, shat-tering occurs via abscission layers that form at the joints(nodes) between successive internodes in the ear. This priorreport and our results suggest that ZmYAB2.1may play a dualrole of regulating both internode elongation and abscissionlayer formation for inflorescence shattering.

Population genetics

Based on multiple tests for departure from neutral evolution,we identified some evidence for selection near the 59 end ofZmYAB2.1, although the evidence is fairly weak. We did notfind a significant departure from neutrality with the Tajima’sD test, nor a test based on coalescent simulations in any ofthe three regions tested; however, we did find a significantdeparture from neutrality at the 59 end of ZmYAB2.1with theHKA test (Table 1). The 59 region also showed the highestloss in nt diversity in maize as compared to teosinte. Failureof Tajima’s D to reject neutrality is not surprising, given that ithas been previously shown that Tajima’s D has low power fordetecting selection in maize (Tenaillon et al. 2004). Similarly,the coalescent simulation test has also been shown to beconservative (Zhao et al. 2011). Nonetheless, with some ev-idence for selection at the 59 end of ZmYAB2.1, the causativesite for the difference between maize and teosinte is likely tobe close to ZmYAB2.1 and within the 68-kb interval definedby the fine-mapping.

We identified four different mutant alleles of ZmYAB2.1while sequencing several maize inbred lines to clarify thegene structure. The first two mutant alleles, B73_Spmand W22_Spm, were initially discovered from sequencingof ZmYAB2.1 in B73 and W22. As described in the previoussection, both B73 and W22 carry unique dSpm insertionsbetween the first and second exons. Simple expression anal-ysis via reverse transcription-PCR (RT-PCR) revealed that theZmYAB2.1 transcript in B73 and W22 are truncated, includ-ing only the first exon (data not shown). We hypothesizedthat the dSpm insertions are responsible for the truncationof the ZmYAB2.1 transcripts. Two other mutant alleles,Mo17_82bp and Ki3_2bp, were discovered from sequencingtranscripts of ZmYAB2.1 in Mo17 and Ki3. As the names

etb1.2, YABBY Class Transcription Factor 1577

suggest, Mo17 carries an 82-bp out-of-frame insertion in thethird exon of ZmYAB2.1, while Ki3 carries a 2-bp out-of-frameinsertion in the third exon of ZmYAB2.1. All four mutantalleles likely result in functional knockout of ZmYAB2.1.

Knowing that maize has multiple mutant alleles ofZmYAB2.1, we surveyed the distribution of these mutantalleles in diverse maize and teosinte populations. We foundthat these four mutant alleles are relatively rare (0–2%) inteosinte populations, while three of the mutant alleles arewidespread (4–22%) in maize populations (Table 2 andTable S4). The frequencies and geographic distributions ofthe mutant allele frequencies suggest that B73_Spm andW22_Spm alleles, which are broadly distributed, arose earlyduring the diversification of maize, while Mo17_82bp andKi3_2bp alleles arose much later and are geographically con-fined (Figure S2). B73_Spm alleles are concentrated around

South America, W22_Spm alleles are prevalent around Cen-tral America, Mo17 alleles are restricted to North America,and the Ki3_2bp allele is known only from that single inbredline.

Gene expression

Given that we earlier hypothesized that a cis-regulatorychange at ZmYAB2.1 is a causal polymorphism underlyingetb1.2, we first analyzed the expression of the ZmYAB2.1maize and teosinte alleles in the two parental lines,T1L_C07 (teosinte allele) and T1L_D17 (W22 maize allele).We found that the ZmYAB2.1W22maize allele (T1L_D17) isnot expressed, while the teosinte allele (T1L_C07) isexpressed at a relatively high level (Figure 2A). This wasthe expected result given that the W22 allele has the dSpminsertion, which should block the synthesis of a stable

Figure 1 Fine-mapping of etb1.2 on chromosome 1L. Genotypes of 45 RC-NILs and two NI-RILs carrying different teosinte introgression within etb1.2are shown on the left. White bars represent maize genotype, black bars represent teosinte genotype, and gray bars represent unknown genotype. Leastsquare means and SE of ear internode lengths for each line are shown on the right. Markers SBM03 and SBM04 flank the narrowed etb1.2 interval,which is expanded at the bottom to show a close-up view of the region. Multiple insertion alleles (B73_Spm, W22_Spm, Mo17_82bp, and Ki3_2bp) areshown in red triangles. NI-RIL, nearly isogenic recombinant inbred lines; RC-NIL, recombinant chromosome nearly isogenic lines.

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transcript. We excluded the possibility of primer mismatch inthe W22 maize allele since the primer sequences matchedperfectly to the W22 maize and teosinte sequences.

As a verification of this expression difference between themaize and teosinte alleles in the parental lines, we comparedthe expression of ZmYAB2.1 in two RC-NILs. One RC-NIL(IN0949) carries a recombinant allelewith themaize regulatoryregion and full-length maize:teosinte recombinant coding re-gion without the dSpm insertion, while the other (IN1207)carries teosinte regulatory and coding regions (Figure S3). In-terestingly, we observed expression of ZmYAB2.1 in both ofthese two lines, but the expression was significantly higher withthe teosinte allele (IN1207) as compared to the recombinantallelewith themaize regulatory region (IN0949) (P=0.00003)(Figure 2B). These results suggest that there is a difference inthe 59 cis-regulatory elements (CREs) of the maize (W22) andteosinte alleles. The maize cis-element engenders lower expres-sion than the teosinte cis-element. The lack of ZmYAB2.1 expres-sion in T1L_D17 with theW22 allele presented above is likely aresult of the dSpm insertion. As shown in Figure 1, T1L_D17 andIN0949 both show themaize class of ear internode length,whileT1L_C07 and IN1207 both show the teosinte class of internodelength. These results suggest that high expression of ZmYAB2.1confers longer ear internode length, while low or null expres-sion of ZmYAB2.1 confers shorter ear internode length.

In addition, we performed another expression analysisusing an allele-specific expression assay in plants heterozy-gous for different combinations of four maize (A619, Oh43,MR12, andMR19) and two teosinte (TIL01 andTIL11) allelesof ZmYAB2.1. We calculated the allele-specific expression bytaking log2 of the ratio of teosinte allele expression level overthe maize allele expression level. We observed higher expres-sion of the teosinte ZmYAB2.1 allele over themaize ZmYAB2.1allele in four of the six F1 hybrids with different allelic combi-nations (Figure 2C). One F1 showed higher expression of theteosinte allele, although the difference was not statisticallysignificantly different from equivalent expression. AnotherF1 showed fully equivalent expression of the maize and teo-sinte alleles (Figure 2C). These results suggest that teosintealleles are generally more highly expressed thanmaize alleles;however, they also show some evidence for functional diversityamong alleles within both maize and teosinte.

Interactions with tb1

Aprevious study suggested that tb1 is epistatic to etb1.2 basedon phenotypic data alone (Studer and Doebley 2011). We

attempted to verify the epistasis by comparing the expressionlevels of ZmYAB2.1 and tb1 to ear internode lengths withrespect to the genotypes at ZmYAB2.1 and tb1. We definedthe four different classes of genotypes at ZmYAB2.1 and tb1as MM, TM, MT, and TT, where the first letter representsmaize (M) or teosinte (T) genotype at ZmYAB2.1 and thesecond letter represents maize (M) or teosinte (T) genotypeat tb1. Among these four classes, we observed a significantinteraction effect between ZmYAB2.1 and tb1 for both earinternode length (P = 0.000107) and ZmYAB2.1 expression(P = 0.003090) (Figure 3). For ZmYAB2.1 expression,the MM and MT classes show no detectable expression asexpected, while the TT class shows significantly higher ex-pression than the TM class (P = 0.0152) (Figure 3A). Thisresult indicates that the genotype at tb1 has an effect onZmYAB2.1 expression. For tb1 expression, the MM and TMclasses show similar expression levels, and the MT and TTclasses show similar expression levels, albeit significantlylower than MM and TM classes (P = 0.00015) (Figure 3B).These observations indicate that ZmYAB2.1 does not affecttb1 expression.

We also tested for significant interaction effects (epistasis)on ear internode length. Here, each of the four genotypicclasses is significantly different from the others, with increas-ing ear internode length starting from MM, TM, MT, to TTclasses (Figure 3C). However, the difference between MMand TM is small as compared to the difference between MTand TT, which is much larger. Using the linear model as de-scribed in Materials and Methods, we identified a significantinteraction term between ZmYAB2.1 and tb1 on ear inter-node length (P = 0.000107). The genotypic means for thefour classes and the significant interaction term suggest thatthe genotype at tb1 (M or T) has a larger effect on trait valuewhen the teosinte allele is present at ZmYAB2.1, but a muchsmaller effect when the maize allele is present at ZmYAB2.1(Figure 3C). Based on these results, we see that the interac-tion between the teosinte ZmYAB2.1 allele and teosinte tb1allele leads to higher ZmYAB2.1 expression, and subse-quently longer ear internode length.

The combination of expression and phenotypic data allowus to propose the following model. Expression level ofZmYAB2.1 is dependent upon both the ZmYAB2.1 and tb1genotypes, while expression level of tb1 is only dependentupon its own genotype. Our observations suggest thatTB1 acts as a repressor of ZmYAB2.1 directly or indirectly(Figure 3D). Thus, we propose that TB1 negatively regulates

Table 1 Results for three different selection tests on three different regions upstream of ZmYAB2.1

Description

Pair-wise HKA test Tajima’s D

Coalescent simulation Diversity loss (%)Maize Teosinte Maize Teosinte

68 kb upstream ZmYAB2.1 0.5877 0.9789 0.9477 20.5836 0.5346 0.286434 kb upstream of ZmYAB2.1 0.9511 0.9812 0.3329 20.5534 0.9830 0.146759 region of ZmYAB2.1 0.0082* 0.4821 0.1057 20.8887 0.4378 0.4375

P values are shown for pair-wise HKA test and coalescent simulation, where the significant P value is marked with an asterisk (*). Tajima’s D statistic is shown for Tajima’s test,however, no significance is found. HKA, Hudson–Kreitman–Aguade.

etb1.2, YABBY Class Transcription Factor 1579

ZmYAB2.1 expression, while ZmYAB2.1 positively regulatesgenes responsible for ear internode elongation (Figure 3D).However, this model is not complete as the difference in earinternode length between the MM and MT classes with nullZmYAB2.1 expression is unexplained. This result suggeststhat an additional pathway exists through which TB1 inde-pendently regulates other genes responsible for ear internodeelongation (Figure 3D).

In situ hybridization

To observe the spatial expression domain of ZmYAB2.1 andany potential difference between the maize and teosinte al-leles of ZmYAB2.1, we performed in situ hybridization on earsof both maize and teosinte alleles in both maize and teosintegenetic background (Figure 4). In our initial hybridizations,we used a mix of antisense probes containing the 39-UTRprobe, and either a coding sequence probe or a 59-UTR probe,under standard hybridization conditions. In maize ears con-taining the maize allele (T1L_D17) (Figure 4A) as well as theteosinte allele (T1L_C07) (Figure 4B), we observed expres-sion in a narrow band of cells subtending the spikelet pair.Similar results were seen in teosinte ears with the maizeallele (TIL09) (Figure 4C) and the teosinte allele (TIL11)(Figure 4D), where expression was clearly localized to thefuture abscission zone between individual fruitcases. Maizeears lack an abscission zone and, considering the dramaticmorphological changes resulting from domestication, it canbe difficult to identify homologous regions in the maize com-pared to teosinte ears. However, given the similar expressionin a narrow band of cells in both maize and teosinte, wereason that the region of expression in maize is homologousto the abscission zone, indicating that a vestigial abscissionzone is present in the maize ear.

Considering that the qPCR results clearly showed a lack ofexpression of the 39 region of ZmYAB2.1 maize allele, wewere surprised that our probe hybridized similarly to bothalleles. One possible explanation for this discrepancy be-tween the qPCR as compared to in situ expression results isthat a closely related paralog (GRMZM2G074124) mightcross-hybridize to our probe. A comparison of the coding se-quence of the paralogs shows that they are 87% identical,

while their 39-UTR region is only 67% identical. Thus, werepeated the hybridizations with a probe specific to the39-UTR while also raising the hybridization temperature toprovide more specificity (Figure 4, E and F and Figure S5). Inteosinte ears, only the teosinte allele showed some weakexpression in the abscission zone (Figure 4F), while themaize allele showed no evidence of expression (Figure 4E).Similar results were seen in maize ears (Figure S5). Theseresults are consistent with the view that the teosinte allele isexpressed in the abscission zone, while the dSpm insertionpresent in the maize allele abolishes expression of the 39 endof the transcript. Given that the internode length was the traitmapped to ZmYAB2.1, we infer that expression in the abscis-sion layer promotes internode elongation. In this regard,Tanaka et al. (2012) have suggested that the rice YABBYgene, TONGARI-BOUSHI, acts noncell autonomously.

Discussion

YABBY gene function and ear internode length

Previous studies in Arabidopsis revealed that members of theYABBY gene family act redundantly to regulate complex pat-terning and growth decisions in lateral organ development(Bowman and Smyth 1999; Eshed et al. 1999; Sawa et al.1999; Siegfried et al. 1999; Villanueva et al. 1999; Bowman2000; Sarojam et al. 2010; Finet et al. 2016). The YABBYgene family consists of five clades, namely CRABS CLAW(CRC), INNER NO OUTER (INO), YABBY3/FILAMENTOUSFLOWER (YAB3/FIL), YABBY2 (YAB2), and YABBY5 (YAB5)(Yamada et al. 2011). FIL and YAB3 act together to promoteleaf growth and expansion, including some aspects of adaxial–abaxial polarity (Siegfried et al. 1999). While YAB2 and YAB5have no apparent loss-of-function phenotype in Arabidopsis,both genes significantly enhance the leaf and meristem filand yab3 phenotypes (Sarojam et al. 2010), indicating thatYAB2 has a redundant role in early leaf growth and patterning.Although limited to floral development, both INO (Villanuevaet al. 1999) and CRC (Alvarez and Smyth 1999; Bowman andSmyth 1999; Eshed et al. 1999) also directly regulate organgrowth and expansion.While YAB2 gene function has not beenas clearly elucidated, it likely contributes to tissue growth andexpansion roles common to all YABBY family members. Inter-estingly, the tissue growth functions of YABBY have beenshown to correlate with TCP (TCP is a family of transcriptionfactors named after tb1, cyc, and PCFs) gene expression(Sarojam et al. 2010). As tb1 is a TCP family member, thiscould help explain the apparent epistatic interaction betweenZmYAB2.1 and tb1.

Studies on YAB2 homologs in other species including rice,sorghum, and maize suggest that YAB2 plays a bigger role infloral organ regulation than just lateral organ polarity spec-ification (Jang et al. 2004; Toriba et al. 2007; Lin et al. 2012).Examples of these YAB2 homologs are OsYAB2 (previ-ously known as OsYABBY1 and OsSh1) in rice, Shattering1(Sh1) in sorghum, and ZmYAB2.5.1 (previously known as

Table 2 Allele distribution of four different mutant alleles ofZmYAB2.1 across diverse maize and teosinte populations

Population

Allele distribution (%)

W22_Spm B73_Spm Mo17_82bp Ki3_2bp

Z. mays ssp. mexicana(Teosinte)

0 1.41 0 0

Z. mays ssp. parviglumis(Teosinte)

1.12 0 0 1.12

Z. mays ssp. mays(Maize Landrace)

11.42 22.05 4.72 0

Z. mays ssp. mays(Maize Inbred)

19.30 17.24 15.52 3.45

All mutant alleles are mutually exclusive, except for one case where TIL09 has bothKi3_2bp and W22_Spm insertions in one allele.

1580 C. J. Yang et al.

ZmSh1-5.1). OsYAB2, Sh1, and ZmYAB2.5.1 function in themaintenance of abscission layers in the inflorescence (Linet al. 2012). In addition, molecular analyses of OsYAB2 didnot identify a role in polarity regulation of lateral organs(Toriba et al. 2007). Ectopic expression of OsYAB2 led to anincrease in stamen and carpel number, with no impact onabaxial–adaxial identity of lateral organs (Jang et al. 2004).Adding a role of ear internode length regulation to the func-tions for members of the YAB2 clade suggests a commontheme for these homologs, that is, regulation of growthwithin flowers and inflorescences. Additionally, all the de-scribed YAB2 homologs are actually domestication genes(Lin et al. 2012), signifying the importance of members ofYAB2 clade in domestication.

etb1.2 as a genetic background factor

As suggested in the name, enhancer of tb1.2, etb1.2 refers to aQTL that modifies the effects of tb1 and contributes to a ge-

netic background in which the effects of tb1 are enhanced.Genes that contribute to genetic background are expectedto interact epistatically with the focal gene (Wade 2002).Consistent with this expectation, our phenotypic and expres-sion data (Figure 3) show that ZmYAB2.1 and tb1 functionnonadditively in the regulation of ear internode length andZmYAB2.1 expression. Using the MM class (homozygousmaize at both ZmYAB2.1 and tb1) as a reference point, theTT class has more than twice the effect on ear internodelength than the MT and TM classes combined. Thus, we seean additive-by-additive epistatic effect (Crow and Kimura1970) between ZmYAB2.1 and tb1. Such an epistatic effect,along with more complicated additive-by-dominance anddominance-by-dominance epistatic effects, are the mechanismsunderlying genetic background (Wade 2002). Consequently,ZmYAB2.1 represents a component of the background thatmodulates the effects of tb1, and the identification ofZmYAB2.1 is the first step in understanding the geneticbackground that is affecting tb1.

The QTL at tb1 was first discovered as a large effect QTLthat controls multiple traits related to both ear and plantarchitecture (Doebley et al. 1990; Doebley and Stec 1993).The observed effects include the regulation of internodelength in both the ear and lateral branches, as well as a largeeffect on inflorescence sex, the partial conversion of the earinto a tassel. The effects of the tb1 QTL are influenced byan epistatic interaction with a second QTL on chromosome3 (Doebley et al. 1995). Notably, when the tb1 QTL wasintrogressed alone into the maize genetic background,the size of the effects on internode length and inflores-cence sex were diminished in comparison with the jointintrogression of the tb1 QTL plus the additional QTL onchromosome 3. This prior demonstration of a QTL on chro-mosome 3, contributing to the genetic background for tb1and our characterization of etb1.2, highlights the impor-tance of genetic background effects for tb1. Consistentwith these observations, Vann et al. (2014) have recentlyshown that the maize allele of the tb1 regulatory regioncan segregate in natural teosinte populations without adetectable effect on tillering, which may indicate geneticbackground effects.

Studer and Doebley (2011) further investigated the tb1QTL by asking whether it would fractionate into multiplelinked QTL. In the 63 cM region surrounding tb1, theseauthors detected four additional QTL affecting ear andinflorescence architecture traits. etb1.2 is one of these fouradditional QTL, and in addition to being closely linked to tb1,etb1.2 is a background gene that modifies the effect of tb1through an epistatic interaction. The close linkage of tb1 andetb1.2 might have been a factor favoring selection on thesetwo genes during domestication. Le Thierry D’Ennequin et al.(1999) have proposed that selection during domesticationcan favor tightly linked genes in outcrossing species whenthere is gene flow between the incipient crop and its wildancestor, conditions that apply to the maize-teosinte domes-tication system.

Figure 2 Expression levels of ZmYAB2.1 in various backgrounds. Teo-sinte ZmYAB2.1 allele (T1L_C07) is expressed at a relatively high level,while maize ZmYAB2.1 allele (T1L_D17) is not expressed (A). Recombi-nant ZmYAB2.1 allele carrying maize W22 regulatory region and teosintegenic region (IN0949) is expressed about threefold lower than a completeteosinte ZmYAB2.1 allele (IN1207) (B). Allele-specific expression ofZmYAB2.1 (C) is shown as log2 of teosinte allele expression level overmaize allele expression level, and significance (P , 0.05) of each com-parison (H0: m = 0) is indicated by an asterisk (*). NI-RIL, nearly isogenicrecombinant inbred lines; RC-NIL, recombinant chromosome nearlyisogenic lines.

etb1.2, YABBY Class Transcription Factor 1581

Nature of selection at ZmYAB1.2

The signal of selection at ZmYAB1.2 is muchweaker than thatobserved with other maize domestication genes. Our fine-mapping places the causative site between the first exonand 68 kb upstream. To assay this region for selection evi-dence, we tested for selection at the distal (268 kb), proxi-mal (234 kb), and 59 region of ZmYAB2.1. We observedselection evidence only in the form of a single significantHKA test at the 59 region (Table 1), which includes the 59-UTR and exon 1. These results are very different from thosewith two other maize domestication genes (tb1 and tga1),both of which show strong selection evidence near their re-spective causative lesions (Wang et al. 2005; Studer et al.2011). The weak signature of selection might indicate thateither (1) the single positive HKA test is a false positive, (2)the 59 region is too distant (relative to the local recombina-tion rate) from the selection target site to detect a strongsignal, or (3) ZmYAB2.1 was the target of a soft sweep ator near its 59 region (Innan and Kim 2004; Hermisson and

Pennings 2005). In a whole genome selection scan, Huffordet al. (2012) did not observe selection evidence at ZmYAB2.1,suggesting either no selection or selection too weak to leave asignature. Overall, the evidence for selection at ZmYAB2.1must be considered weak at best.

Assuming that the significant HKA test for the 59 region isnot a false positive, the likely target of selection would be anupstream CRE. Exon 1 lacks nonsynonymous differences be-tween our maize and teosinte alleles, so exon 1 seems un-likely to harbor the causative polymorphism. The significantHKA test for the 59 region would then reflect selection on alinked CRE. We see no evidence for selection at the 234 kbproximal region, thus the proposed CRE should lie betweenthis proximal region and exon 1. The lack of selection evi-dence at the proximal region can be explained because thisregion shows very little linkage disequilibrium with the 59region (Figure S9), and thus selection on a CRE close to the59 region is not apt to have left a signature of selection at theproximal region. Due to the presence of multiple transpos-able elements (Figure 1) and the repetitive nature of theirsequences, we were unable to develop any PCR primer sets toassay sites between the first exon and the 234 kb proximalregion.

Although the evidence for selection on a CRE upstream ofZmYAB2.1 is weak, this evidence is augmented by the obser-vation that a sample of multiple teosinte alleles of ZmYAB2.1are expressed more highly than a sample of multiple maizealleles, consistent with the hypothesis that selection acted ona CRE. Our expression data for three teosinte and five maizelines show the teosinte allele to be expressed higher than themaize allele in all comparisons except Oh43 3 TIL01, forwhich case equivalent expression was observed (Figure 2).Lemmon et al. (2014) reported allele-specific expression atZmYAB2.1 for three maize and three teosinte lines, and theirdata also show that the teosinte alleles are expressed at ahigher level than the maize allele (Table S7). Overall, thesedata suggest that maize was under selection for reduced ex-pression of ZmYAB2.1.

If selection was acting on a CRE to reduce ZmYAB2.1 ex-pression, this may explain why four distinct loss-of-functionalleles (LOF) are present in maize, some at moderately highfrequencies (Table 2). Each of these LOF alleles occurs at lowfrequency in teosinte (�0–2%) but higher frequency inmaizelandraces or inbreds (�4–22%). The LOF alleles are geo-graphically distributed in maize outside the initial area ofmaize domestication in southwest Mexico (van Heerwaardenet al. 2011), suggesting that they arose postdomestication(Figure S2). Their presence at low frequency in teosintecan be explained by introgression from maize into teosinte.The presence of a mix of low-expression and LOF alleles inmaize suggests that loss/reduction of ZmYAB2.1 function isbeneficial in maize. Reduced or lost function would produceshorter internodes in the ear andmore tightly packed kernels.Together, the selection tests, expression data, and LOF allelescan be explained by a model that a CRE at ZmYAB2.1 wasinitially selected for lower expression and then, as maize

Figure 3 Expression levels of ZmYAB2.1 (A) and tb1 (B), and ear inter-node lengths (C) of four different genotype classes. Genotype ofZmYAB2.1 is denoted on the left while genotype of tb1 is denoted onthe right (e.g., MT = maize allele at ZmYAB2.1 and teosinte allele at tb1).Expression level of ZmYAB2.1 is affected by tb1 genotype (A); however,expression level of tb1 is unaffected by ZmYAB2.1 genotype (B). Pearson’scorrelation is 0.72 between ZmYAB2.1 expression level and ear internodelength, and 20.69 between tb1 expression level and ear internode length.Based on the expression and phenotype data, the most plausible model(D) involves two independent pathways. The first pathway begins withTB1 as a negative regulator of ZmYAB2.1, while ZmYAB2.1 is a positiveregulator of ear internode length (as seen with the effect of tb1 expres-sion level on ZmYAB2.1 expression level). The second pathway occursindependent of ZmYAB2.1 as TB1 negatively regulates ear internodelength (as seen with the effect of tb1 expression level on ear internodelength when ZmYAB2.1 expression is null). All interactions suggestedhere could be direct or indirect.

1582 C. J. Yang et al.

spread out of it center of origin, multiple independent LOFalleles accumulated in different geographic regions.

The presence of multiple LOF alleles at ZmYAB2.1 is rem-iniscent of allelic diversity at some other domestication-related genes. In maize, five different LOF alleles of thesugary1 (su1) gene have been identified to confer sweetnessin sweet maize varieties (Tracy et al. 2006). Similar toZmYAB2.1 alleles (Figure S2), these five different su1 allelesare also widely distributed based on geographical origin ofeach allele (Tracy et al. 2006). In addition, three differentLOF alleles have been observed at six-rowed spike 1 (Vrs1) inbarley that converts two-rowed barley into six-rowed barley

(Komatsuda et al. 2007). Multiple LOF alleles of waxy (wx)have been identified to confer variation in amylose content inrice, resulting in glutinous and nonglutinous varieties of rice(Chen et al. 2014). Multiple LOF alleles of 5-O-glucosyltrans-ferase have been found to regulate anthocyanin contents ingrapes (Yang et al. 2014). These examples stand in contrastto domestication genes like tb1 (Studer et al. 2011) and tga1(Wang et al. 2005), which have single domestication allelesthat provide a gain-of-function.

Pleiotropy at ZmYAB2.1

Aside from ear internode length regulation, ZmYAB2.1 hasalso been previously suggested to regulate ear shattering (Linet al. 2012). Such a pleiotropic effect at ZmYAB2.1 is notsurprising, given that ear internode elongation and shatter-ing are biologically related traits (Khan et al. 2012). Theshattering trait is interpreted as the separation of two organsat an abscission zone, and it is an important mode of seeddispersal in wild species (Roeder and Yanofsky 2005). InArabidopsis, BREVIPEDICELLUS (BP), KNOTTED-LIKE FROMARABIDOPSIS THALIANA 1 (KNAT1), KNAT2, and KNAT6 aregenes known to be involved in both inflorescence internodeelongation and abscission zone formation (Smith and Hake2003; Ragni et al. 2008; Shi et al. 2011; Khan et al. 2012).Typically, abscission zone formation is restricted until seedmaturity. However, upon seedmaturity, both cell size and num-ber increase at the abscission zone alongwith lignin deposition(Shi et al. 2011), resulting in the shattering. The increase incell size and number would lead to internode elongation.

Despite ZmYAB2.1 being a pleiotropic gene, we did notidentify any significant variation for the shattering trait in allthe RC-NILs (data not shown). This result can be due to severalreasons. Given that ZmYAB2.1 is a gene of small effect, phe-notyping accuracy can be crucial to tease the small differenceapart. Unfortunately, our simple drop test method for scoringthe shattering trait is not as accurate as our method for scoringear internode length. In addition, as discussed previously, ge-netic background plays an important role for characterizing agene and our fine-mapping population lacks the correct genet-ic background for the shattering trait to be expressed. Also, ofthe two shatteringQTL that were previouslymapped inmaize,the LOD score for the QTL on chromosome 1 containingZmYAB2.1 is about five times lower than the QTL on chromo-some 5 (Lin et al. 2012), indicating that ZmYAB2.1 has amuchsmaller effect of these two QTL.

Overview

We have taken a phenotype-to-gene mapping approach tobetter understand the nature of a factor, etb1.2, that contrib-utes to the genetic background affecting the phenotypic ef-fects of tb1. Surprisingly, or perhaps not, the gene underlyingthe factor was revealed to be a DNA-binding transcriptionalregulator, ZmYAB2.1, like tb1 itself. Transcription factors ap-pear to be the most common class of genes involved in theevolution of morphological traits during domestication(Doebley 2006). Another surprise was that ZmYAB2.1 had

Figure 4 In situ RNA hybridization of ZmYAB2.1 in maize and teosinte.(A–D) were hybridized with an antisense probe including both the codingsequence and 39-UTR. (A) Maize ear with the maize allele of ZmYAB2.1.(B) Maize ear with the teosinte allele. Expression is observed in a narrowband subtending the spikelet pair. This same probe hybridized to teosinteears with the maize allele (C) or the teosinte allele (D) also show a narrowband of expression in the future dehiscence zone. A probe specific to the39-UTR hybridized to teosinte ears with the maize allele (E) or the teosinteallele (F) only showed weak expression in the dehiscence zone with theteosinte allele. Arrows indicate dehiscence zone in teosinte ears (C–F) andthe homologous region in maize (A and B). The scale bars in all panels are200 mm.

etb1.2, YABBY Class Transcription Factor 1583

been previously identified as a likely gene contributing to thechange from shattering to nonshattering ears during maizedomestication (Lin et al. 2012). In addition to etb1.2, thereare additional known factors that interact epistatically withtb1 to affect multiple domestication traits (Doebley et al.1995; Studer and Doebley 2011). The apparent intersectionof multiple genes working in concert to regulate a suite oftraits altered during domestication hints that selection duringdomestication operated to modify the function of a complexdevelopmental network. Identifying other players in this net-work will be key in understanding how genes like tb1 and thebackground in which they work have evolved in concert.

Acknowledgments

We thank Jordan Michels, Zhihong Lang, Zachary Lemmon,Jesse Rucker, Elizabeth Buschert, and Eric Rentmeester forassistance throughout the entire project. This work wassupported by National Science Foundation funding (grantsIOS1025869, IOS0820619, and IOS1238014).

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Communicating editor: K. Bomblies

etb1.2, YABBY Class Transcription Factor 1585

Figure S1. Schematic of the process of generating RC-NILs for phenotyping. Two parental lines

were initially selected to each carry a maize allele and a teosinte allele at etb1.2. Cross between

these two parental lines yielded F1 which was heterozygous at etb1.2. The F1 was then selfed to

produce F2, where most of the F2 plants were non-recombinants and only a small fraction of the

F2 plants were recombinants. These recombinants were identified using markers listed in Table

S1, and further selfed to produce homozygous recombinants that would eventually become the

RC-NILs used in phenotyping.

Figure S2. Geographical distributions of 3 null ZmYAB2.1 alleles: W22_Spm [A], B73_Spm [B],

Mo17_82bp [C] in maize landrace populations. Ki3_2bp is not shown here as it is only present in

2 maize inbreds and 1 teosinte inbred.

Figure S3. Cartoon showing recombination breakpoint of a RC-NIL (IN0949) in between Exon 1

and Exon 2 of ZmYAB2.1. IN0949 carries maize regulatory region and mostly teosinte coding

region, while IN1207 carries full teosinte regulatory and coding regions. IN0949 displays maize

class ear internode length while IN1207 displays teosinte class ear internode length (Figure 1).

IN0949 also has lower ZmYAB2.1 expression in comparison to IN1207 (Figure 2B).

Figure S4: Genotypes of 20 NI-RILs used in testing interaction between ZmYAB2.1 and tb1.

Markers are listed by chromosome and physical position (AGP_v2). All 20 NI-RILs have maize

W22 genotype outside of the marker interval shown here.

Figure S5. ZmYAB2.1 3’UTR probe hybridized to maize ears containing the maize allele (A) or

the teosinte allele (B). Weak expression in the region homologous to the dehiscence zone

(arrows) is only seen in in the teosinte allele (B). Scale bars are 200µm.

Figure S6. Reordering of B73 AGPv2 reference genome contigs around GRMZM2G085873. A

snippet of maize B73 reference genome around chromosome 1, positions 260,361,563 –

260,395,575, is shown on top. Sequence from this region is aligned to our complete

GRMZM2G085873 sequence using Mauve 2.4.0 (Darling et al. 2004). Red blocks represent the

alignment from exon 2 to exon 6 between our sequence and the reference genome. Green blocks

represent the alignment from 5’ UTR to part of intron 1 between our sequence and the reference

genome. Intron 1 either has multiple sequence errors in the reference genome or a portion

missing from the reference genome. Based on our sequencing results, the contig spanning chr1:

260,377,789-260,388,002 should be oriented in an opposite direction. Also, those contigs

spanning chr1: 260,367,665-260,377,688 should be moved elsewhere, either upstream or

downstream of GRMZM2G085873.

10 20 30 40 50 60 70 80 | | | | | | | |

B73 AGCTGAGGAAGGCAATTGTTTCGAATCTGGCTGCTGCGTAGCCAGACATAGAGAATCCTTTTAGACGCAACGAGGCCCCA

W22 AGCTGAGGAAGACAATTGTTTCGAATCTGGCTGCTGCGTAGCCAGACATAGAGAATCCTTTTAGACGCAACGAGGCCCCA

Teo ACCTGAGGAAGGCAATTGTTTCGAATCTGGCTGCTGCGTAGCCAGACATAGAGAATCCTTTTAGACGCAACGAGGCCCCA

90 100 110 120 130 140 150 160

| | | | | | | |

B73 GCAATTCATTGTGGCGTTTTGCACCCACATCAACCCGCCGCGCCCCCCTTCACAATAGGAGCCAGAACAAAAAAAAAA--

W22 GCAATTCATTGTGGCGTTTTGCACCCACATCAACCCGCCGCGCCCCCCTTCACAATAGGAGCCAGAACAAAAAAAAAA--

Teo GCAATTCATTGTGGCGTTTTGCACCCACATCAACCCGCC-------CCTTCACAATAGGAGCCAGAACAAAAAAAAAAAC

170 180 190 200 210 220 230 240

| | | | | | | |

B73 ---CCACTACTCCTCTCTCTCCCTCACACACA--GAGTGAGAGAGAGAGAGACGCACAAGAAATCAGTAGGTCAATCTCT

W22 ---CCACTACTCCTCTCTCTCCCTCACACACA--GAGTGAGAGAGAGAGAGACGCACAAGAAATCAGTAGGTCAATCTCT

Teo CCCCCACTACTC-----TCTCTCTCACACACACATAGAGAGAGAGAGAGAGACGCACAAGAAATCAGTAGGTCAATCTCT

250 260 270 280 290 300 310 320

| | | | | | | |

B73 CCTCTCACCTTTACATCGTCTTTGTATTTACAAAGTTGTTGATGACTTGATGTAGAGGCTGTACCTGCTGGTTTGCTACC

W22 CCTCTCACCTTTACATCGTCTTTGTATTTACAAAGTTGTTGATGACTTGATGTAGAGGCTGTACCTGCTGGTTTGCTACC

Teo CCTCTCACCTTTACATCGTCTCTGTATTTGCAAAGTTGTTGATGAGTTGATGTAGAGGCTGTACCTGCTGGTTTGCTACC

330 340 350 360 370 380 390 400

| | | | | | | |

B73 CCTAGCTAGCTATATGTCGTCCAATCCTAGTTGAGCTCTCGATCTGGATGGCCCTCTGATTAGTCGTCCTTTGCTTGGTG

W22 CCTAGCTAGCTATATGTCGTCCAATCCTAGTTGAGCTCTCGATCTGGATGGCCCTCTGATTAGTCGTCCTTTGCTTGGTG

Teo CCTAGCTAGCTATATGTCGTCCAATCCTAGTTGAGCTCTCGATCTGGATGGCCCTCTGATTAGTCCTCCTTTGCTTGGTG

410 420 430 440 450 460 470 480

| | | | | | | |

B73 GTGCCTGCTGTGTATATCGCCACCCCGGCCGCTTGCTTCCGCTAGTAGCTAGTCCCTT--CTTCTCTTCTCGATCAGTTT

W22 GTGCCTGCTGTGTATATCGCCACCCCGGCCGCTTGCTTCCGCTAGTAGCTAGTCCCTT--CTTCTCTTCTCGATCAGTTT

Teo GTGCCTGCTGTGTATATCGCCACCCCGGCCGCTTGCTTCCGCTAGT-------CCCTTTTCTTCTCTTCTCGATCA----

490 500 510 520 530 540 550 560

| | | | | | | |

B73 GTTTAATTGGCAGAGCGTATGTATCACACCCAAGGCGAAGGAATGTCGTCGGCGGCCCAGATCGCGCCGGTGCCGGAGCA

W22 GTTTAATTGGCAGAGCGTATGTATCACACCCAAGGCGAAGGAATGTCGTCGGCGGCCCAGATCGCGCCGGTGCCGGAGCA

Teo -----ATTGGCAGAGCGTATGTATCACACCCAAGGCGAAGGAATGTCGTCGGCGGCCCAGATCGCGCCGGTGCCGGAGCA

570 580 590 600 610 620 630 640

| | | | | | | |

B73 TGTATGCTACGTGCACTGCAACTTCTGCAACACAATTCTCGCGGTAATATATATATAACCCCGATCTGATCTCTAGCTAG

W22 TGTATGCTACGTGCACTGCAACTTCTGCAACACAATTCTCGCGGTAATATATATATAACCCCGATCTGATCTCTAGCTAG

Teo TGTGTGCTACGTGCACTGCAACTTCTGCAACACAATTCTCGCGGTAATA--------ACCCCGATCTGATCTCTAGCTAG

650 660 670 680 690 700 710 720

| | | | | | | |

B73 CTAGCCTCTCGATCTGTTTGCCCCCCTCAGCTCGTTTTCTCCCCCGGCTCTCTCGATCGGTTTTTTCCCTGGTTGCCGGC

W22 CTAGCCTCTCGATCTGTTTGCCCCCCTCAGCTCGTTTTCTCCCCCGGCTCTCTCGATCGGTTTTTTCCCTGGTTGCCGGC

Teo C----CTCTCGATCTGTTTGCCCCCCTCAGCTCGTTTTCTCCCCCGGCCCTCTCGATCGGTTT---CCCTGGTTGCCTGC

730 740 750 760 770 780 790 800

| | | | | | | |

B73 CTCTGAACTGAAGCGCAGCTATATATATACAGCAGAAAGATGCATGAAAAAT----AGTTGATTGGCTGCGTGCGCCTGC

W22 CTCTGAACTGAAGCGCAGCTATATATATACAGCAGAAAGATGCATGAAAAAT----AGTTGATTGGTTGCGTGCGCCTGC

Teo CTCTGAACTGAAGCGCAGCTATATATATACAGCAGAAAGATGCATGAAAAATGGTTAGTTGATTGGCTGCGTGCGCCTGC

810 820 830 840 850 860 870 880

| | | | | | | |

B73 ATCGTCGTCGTATAGTAGTTAGTTTTAATTTGTACGAATTGGCTGTTCCGCTTTCTGGATTTCATCAGCTTCTGGATCAT

W22 ATCGTCGTCGTATAGTAGTTAGTTTTAATTTGTACGAATTGGCTGTTCCGCTTTCTGGATTTCATCAGCTTCTGGATCAT

Teo ATCGTCGT---AT---AGTTAGTTTTAATTTGTGCGATTTGGCTGTTCCGCTTTCTGGATTTCATCAGCTTCTGGATCAT

890 900 910 920 930 940 950 960

| | | | | | | |

B73 TTCATCAGAGCTTTTGGTCTAAATTGAAAATTGGGGATGGTTCTTGCATCAAATTAAGTCTTGGGAACAAGATGACTAAT

W22 TTCATCAGAGCTTTTGGTCTAAATTGAAAATTGGGGATGGTTCTTGCATCAAATTAAGTCTTGGGAACAAGATGACTAAT

Teo TTCATCAGAGCTTTTGGTCTAAATTGAAAATTGGGGATGGTTCTTGCATCAAATTAAGTCTTGGGAACAACATGACTAAT

970 980 990 1000 1010 1020 1030 1040

| | | | | | | |

B73 TAGCTAGCAGTCTTAGACGAA----GCTAGTCAGCAAGTTCTCAAAGCATTCTCGCCTTCTGCAGG--CCCTCCTGTACT

W22 TAGCTAGCAGTCTTAGACGAA----GCTAGTCAGCAAGTTCTCAAAGCATTCTCGCCTTCTGCAGG--CCCTCCTGTACT

Teo TAGCTAGCAGTCTTAGACGAATGAAGCTAGTCAGCAAGTTCTCAAAGCATTCTCGCCTTCTGCATGCCCCCTCCTGTACT

1050 1060 1070 1080 1090 1100 1110 1120

| | | | | | | |

B73 TTTTTT-CTGGGAAATTATCGTCATCATCGTTGATGAG--TGGTGGTCTTTATATTAATTAGATCTCAAGTATATATAGC

W22 TTTTTT-CTGGGAAATTATCGTCATCATCGTTGATGAG--TGGTGGTCTTTATATTAATTAGATCTCAAGTATATATAGC

Teo TTTTTTTCTGGGAAATTATCGTCATCATCGTTGATGAGAGTGGTGGTCTATATATTAATTAGATCTCAAGTATATATAGC

1130 1140 1150 1160 1170 1180 1190 1200

| | | | | | | |

B73 TTTGTTTGCCTGCAGGTGTATATGTGTGATATTTGTACATATATAAAGCTTCTTCAGCACCGCGCTAGCTAGCTTCTTTC

W22 TTTGTTTGCCTGCAGGTGTATATGTGTGATATTTGTACATATATAAAGCTTCTTCAGCACCGCGCTAGCTAGCTTCTTTC

Teo TTTGTTTGCCTGCAGGTGTAT--GTGTGATGTGTACATATATATAAAGCTTCTTCAGCACCGCGCTAGCTAGCTTCTTTC

1210 1220 1230 1240 1250 1260 1270 1280

| | | | | | | |

B73 GCATAACCAGAGTCCGATCCGGCCGGTGGTTAACTTATTTCAGCTAATATTTGAAACACAATACATGTATACGACATA--

W22 GCATAACCAGAGTCCGATCCGGCCGGTGGTTAACTTATTTCAGCTAATATTTGAAACACAATACATGTATACGACATA--

Teo GCATAACCAGAGTCCGATCCGGCCGGTGGTTAACTTATTTCAGCTAATATTTGAAACACAATACTTGTATACGACATATA

1290 1300 1310 1320 1330 1340 1350 1360

| | | | | | | |

B73 --GGGCAATAGATTTACTCGTTACCTTCTTGATCGAACTGCAACAGAGAGTGCATGCAATAATAACCATTTTTAAGACGA

W22 --GGGCAATAGATTTACTCGTTACCTTCTTGATCGAACTGCAACAGAGAGTGCATGCAATAATAACCATTTTTAAGACGA

Teo TAGGGCAATAGATTTACTCGTTACCTTCTTGATCGAACTGCAACAGAGAGTGCATGCAATAATAACCATTTTTAAGACGA

1370 1380 1390 1400 1410 1420 1430 1440

| | | | | | | |

B73 TAAAAAAATTTAGTGTGTACCGCGTGGATCTTTAGCTTTTATGCTTTTCTATATAATATGTAGACCATATCTCACATAAA

W22 TAAAAAAATTTAGTGTGTACCGCGTGGATCTTTAGCTTTTATGCTTTTCTATATAATATGTAGACCATATCTCACATAAA

Teo TAAAAAAATT-AGTGTGTACCGCGTGGATCTTTAGCTTTTATGCTTTTCTATATAATATGTAGACCATATCTCACATAAA

1450 1460 1470 1480 1490 1500 1510 1520

| | | | | | | |

B73 TCGAGGTCGTGTCCTAATAAATATACTAAGAGATATATTGACTAAAACAAAGGCCTTTTT--TATTGACTAATAATAATA

W22 TCGAGGTCGTGTCCTAATAAATATACTAAGAGATATATTGACTAAAACAAAGGCCTTTTT-TTATTGACTAATAATAATA

Teo TCGAGGTCGTGTCCTAATAAATATACTAAGAGATATATTGACTAAAACAAAGGCCTTTTTGTTATTGACTAATAATAATA

1530 1540 1550 1560 1570 1580 1590 1600

| | | | | | | |

B73 ACATATATACTCCCTCCGTTTCTTTTTAGTTGTCGATGAATAGTTTAATTTTGCACTATCCAGCGACAAGTAAAACGAAA

W22 ACATATATACTCCCTCCGTTTCTTTTTAGTTGTCGCTGAATAGTTTAATTTTGCACTATCCAGCGACAACTAAAACGAAA

Teo ACATATATA-----------------------------------------------------------------------

1610 1620 1630 1640 1650 1660 1670 1680

| | | | | | | |

B73 CAGAGGGAGTATTAATTATTGCCCAATATCTGTACTACGTCGTAGCATTGCTACTTTTTATTCATATATT-GGAACAAAA

W22 CCGAGGGAGTATTAATTATTGCCCAATATCTGTACTACGTCGTAGCATTGCTACTTTTTATTCATATATT-GGAACAAAA

Teo ------------------TTGCCCAATATCTGTAC---GTCGTAGCATTGCTACTTTTTATTCATATATTTGGAACAAAA

1690 1700 1710 1720 1730 1740 1750 1760

| | | | | | | |

B73 AAAA-TTCCGTTACGTAAGGTTTTTATTCATTGGAACCAAAAAAATATTGTTACGTAAGTTTGAGTACGGTTTGCTGGAA

W22 AAA--TTCCGTTACGTAAGGTTTTTATTCATTGGAACCAAAAAAATATTGTTACGTAAGTTTGAGTACGGTTTGCTGGAA

Teo AAAAA--CCGTTACGTAAGGTTCTTATTCATTGGAACCAAAAAA-T-TTGTTACGTAAGTTTGAATACGGTTTGCTGGAA

1770 1780 1790 1800 1810 1820 1830 1840

| | | | | | | |

B73 AGAGTTTTAAAATGATAAAAAATGTTGATGTGGAATAATTAATAAACGAAGAGCGAACATATCATTACAAAATATGTATA

W22 AGAGTTTTAAAATGATAAAAAATGTTGATGTGGAATAATTAATAAACGAAGAGCGAACATATCATTACAAAATATGTATA

Teo AGAGTTTTAAAATGATCAAAAATGTTGATGTGGAATAATTAATAAACGAAGAGCGAACGTATCACTACAAAAGATGTATA

1850 1860 1870 1880 1890 1900 1910 1920

| | | | | | | |

B73 TATAGTGTACTGCCGATGACCTGGCCGCCCAAAAAATCCAAATCATTACTTCATGATTTTTCTTTTACTTGTTTTGCTTT

W22 TATAGTGTACTGCCGATGACCTGGCCGCCCAAAAAATCCAAATCATTACTTCATGATTTTTCTTTTACTTGTTTTGCTTT

Teo TATAGTGTACTGCCGATGACCTGGCCGCCCAAAAAATCCAAATCATTACTTCATGATTTTTCTTTTACTTGTTTTGCTTT

1930 1940 1950 1960 1970 1980 1990 2000

| | | | | | | |

B73 TTTCATGGCCCTGAGGTTTTTTT-ACATTTTCTTTTTTGGATTTCCTAGTTTACCAAGTTTCTGCTCGGTAGATTATATA

W22 TTTCATGGCCCTGAGGTTTTTTTTACATTTTCTTTTTTGGATTTCCTAGTTTACCAAATTTCTGCTCGGTAGATTATATA

Teo TTTCATGGCCCTGAGGTTTTTTTTACATTTTCTTTTTTGGATTTCCTAGTTGACCAAGTTTATGCTCGGTAGATTATATA

2010 2020 2030 2040 2050 2060 2070 2080

| | | | | | | |

B73 TACTCCATCGGTCTCAAATAAGTATTCGTTTTAACTCTTATTTTTATGTCTATATATTTAAATAAATGATGATGAATCTA

W22 TACTCCATCGGTCTCAAATAAGTATTCGTTTTAACTCTTATTTTTATGTCTATAT--TTAAATAAATGATGATGAATCTA

Teo TACTCCATCGGTCTCAAATAAGTATTCGTTTTTACTCTTATTTTTATATCTATAT--TTAAATAAATGATGATGAATCTA

2090 2100 2110 2120 2130 2140 2150 2160

| | | | | | | |

B73 GACACATATACAAAACACATATATTAATTATTGTATGAACCTATTAAAAAGCTAAAACAGATTTTATCATATTCCACAAA

W22 GACACATATACAAAACACATATATTAATTATTGTATGAACCTATTAAAAAGCTAAAACAGATTTTATCATATTCCAGAAA

Teo GACACATATACAAAACACATATATTAATTATTGTATGAACCTATTAAAAAGCTAAAACAGATTTTATCATATTCCACAAA

2170 2180 2190 2200 2210 2220 2230 2240

| | | | | | | |

B73 CCTTAGTGACAATGAATACAAGGAAAATAAAATACTCCGCTATGCTAAAACATTATTCACGTACGTGTTCTCAAAAGAAC

W22 CCTTAGTGACAATGAATACAAGGGAAATAAAATACTCCGCTATGCTAAAACATTATTCACGTACGTGTTCTCAAAAGAAC

Teo CCTTAGTGACAATGAATACAAGGAAAATAAAATACTCCGCTATGCTAAAACATTATTCACGTACGTGTTCTCAAAAGAAC

2250 2260 2270 2280 2290 2300 2310 2320

| | | | | | | |

B73 TAATTAAGGAGGTGTTCTTGCAAATTATACAGAATTTATGCTTAGTTTTTCTTCAATTAATTTCCTTTTTCTATCGTTGT

W22 TAATTAAGGAGGTGTTCTTGCAAATTATACAGAATTTATGCTTAGTTTTTCTTCAATTAATTTCCTTTTTCTATCGTTGT

Teo TAATTAAGGAGGTGTTCTTGCAAATTATACAGAATTTATGCTTAGTTTTTCTTCAATTAATTTCCTTTTTCTATCGTTGT

2330 2340 2350 2360 2370 2380 2390 2400

| | | | | | | |

B73 TCTCTTGTGCATTAAATTAGTGTTCATAGTTCTTTATCCACATGGTCAACCTCTCTTCATGGAATCATGGATGGGGCGGT

W22 TCTCTTGTGCATTAAATTAGTGTTCATAGTTCTTTATCCACATGGTCAACCTCTCTTCATGGAATCATGGATGGGGCGGT

Teo TCTCTTGTGCATTAAATTAGTGTTCATAGTTCTTTATCCACATGGTCAACCTCTCTTCATGGAATCATGGATGGGGCGGT

2410 2420 2430 2440 2450 2460 2470 2480

| | | | | | | |

B73 GATAGATAACCCATCATCATCGCCATTTCAGTGTAAACATTGCCTATAGGTTTGCAGTTGTTAAAGAAAACAACATATTA

W22 GATAGATAACCCATCATCATCGCCATTTCAGTGTAAACATTGCCTATAGGTTTGCAGTTGTTAAAGAAAACAACATATTA

Teo GATAGATAACCCATCATCATCGCCATTTCAGTGTAAACATTGCCTATAGGTTTGCAGTTGTTAAAGAAAACAACATATTA

2490 2500 2510 2520 2530 2540 2550 2560

| | | | | | | |

B73 CATCTAGCTTATTCATTATTCATCATGAAGTTCTTCCTTTCTAGCTAGCTTATTCATTCTAACTTCTCGCGCCGTAGTTA

W22 CATCTAGCTTATTCATTATTCATCATGAAGTTCTTCCTTTCTAGCTAGCTTATTCATTCTAACTTCTCGCGCCGTAGTTA

Teo CATCTAGCTTATTCATTATTCATCATGAAGTTCTTCCTTTCTAGCTAGCTTATTCATTCTAACTTCTCGCGCCGTAGTTA

2570 2580 2590 2600 2610 2620 2630 2640

| | | | | | | |

B73 CAGGGAATCCATACATCAAAGGGTACAGATTCCTTCTTGTACTATTTATGTTTGCGTCTAGCATGCATACTAGTAGAGAA

W22 CAGGGAATCCATACATCAAAGGGTACAGATTCCTTCTTGTACTATTTATGTTTGCGTCTAGCATGCATACTAGTAGAGAA

Teo CAGGGAATCCATACATCAAAGGGTACAGATTCCTTCTTGTACTATTTATGTTTGCGTCTAGCATGCATACTAGTAGAGAA

2650 2660 2670 2680 2690 2700 2710 2720

| | | | | | | |

B73 ATTATGGGCTTCAGTCCTTGTTGGGATCTGGATTTTCTCAACCGGGAATAATTAGAACAAGTCTCTCGTATGTGCGATTC

W22 ATTATGGGCTTCAGTC-TTGTTGGGATCTGGATTTTCTCAACCGGGAATAATTAGAACAAGTCTCTCGTATGTGCGATTC

Teo ATTATGGGCTTCAGTCCTTGTTGGGATCTGGATTTTCTCAACCGGGAATAATTAGAACAAGTCTCTCGTATGTGCGATTC

2730 2740 2750 2760 2770 2780 2790 2800

| | | | | | | |

B73 GTAGGACTCAAACCCAAACCTCATGTATTGTCTCGCGCTTAGCTTTATTTTAACTATATGGATAATATGTTTTTCTTTTA

W22 GTAGGACTCAAACCCAAACCTCATGTATTGTCTCGCGCTTAGCTTTATTTTAACTATATGGATAATATGTTTTTCTTTTA

Teo GTAGGACTCAAACCCAAACCTCATGTATTGTCTCGCGCTTAGCTTTATTTTAACTATATGGATAATATGTTTTTCTTTTA

2810 2820 2830 2840 2850 2860 2870 2880

| | | | | | | |

B73 AACTGACCCAGAAAAGACCCTTGAGTCTCGTTTGATAATGTTGGACTAAAGATCCTCCAACGTCCAGCTCTTATTAGTCC

W22 AACTGACCCACAAAAGACCCTTGAGTCTCGGTTGATAATGTTGGACTAAAGATCCTCCAACGTCCAGCTCTTATTAGTCC

Teo AACTGACCCACAAAAGACCCTTGAGTCTCGGTTGATAATGTTGGACTAAAGATCCTCCAACGTCCAGCTCTTATTAGTCC

2890 2900 2910 2920 2930 2940 2950 2960

| | | | | | | |

B73 TAAAAAAGTCAAACCAGGACTGATATGCTTACTGGAGACGTGTTCTTTGTCGAGTGCCTCAGACACTCGACAAACGTTTT

W22 TAGAAAAGTCTAACCAGGACTGATATGCTTACTGGAGACGTGTTCTTTGTCGAGTGCCTCAGACACTCGACAAACGTTTT

Teo TAGAAAAGTCTAACCAGGACTGATATGCTTACTGGAGACGTGTTCTTTGTCGAGTGCCTCAGACACTCGACAAACGTTTT

2970 2980 2990 3000 3010 3020 3030 3040

| | | | | | | |

B73 ACCGAGTGCCGCACTCTGCAAAGAGATCTAG-TAAAAAAATCATCGGCGAAAGATTCTTTGTCGAGTGCTTTCTATCGGG

W22 ACCGAGTGCCGCACTCTGCAAAGAGATCTAGGTAAAAAAATCATCGGCGAAAGATTCTTTGTCGAGTGCTTTCTATCGGG

Teo ACCGAGTGCCGCACTCTGCAAAGAGATCTAGGTAAAAAAATCATCGGCGAAAGATTCTTTGTCGAGTGCTTTCTATCGGG

3050 3060 3070 3080 3090 3100 3110 3120

| | | | | | | |

B73 CACTCGGTAAAGACTTTGCCGAGTGCTGAATACATACTCGGCAAAGAAAAGGTGTCATGACAATATGTGCCATGTATGGA

W22 CACTCGGTAAAGACTTTGTCGAGTGCTGAATACATACTCGGCAAAGAAAAGGTGTCATGACAATATGTGCCATGTATGGA

Teo CACTCGGTAAAGACTTTGCCGAGTGCTGAATACATACTCGGCAAAGAAAAGGTGTCATGACAATATGTGCCATGTATGGA

3130 3140 3150 3160 3170 3180 3190 3200

| | | | | | | |

B73 GTATTTGTCGAGTATCTAGGTGTGTCACTCGGCAAAGCAAGGTTTGAAATGGCCCACGCAACGGATTTATTGTCGAGTGC

W22 GTCTTTGTCGAGTATCTAGGTGTGTCACTCGGCAAAGTAAGGTTTGAAATGGCCCACGCAACGGATTTATTGTCGAGTGC

Teo GTCTTTGTCGAGTATCTAGGTGTGTCACTCGGCAAAGCAAGGTTTGAAATGGCCCACGCAACGGATTTATTGTCGAGTGC

3210 3220 3230 3240 3250 3260 3270 3280

| | | | | | | |

B73 TATGGCCTGCCGCACTCGGCAAAAGGTGGCGACTTTGCTGAGTGCCTTGGCGGCACGGGATAAAGCAAGTTTCTAGGTGC

W22 TATGGCCTGCCGCACTCGGCAAAAGGTGGCGACTTTGCTGAGTGCCTTGGCGGCACTGGATAAAGCAAGTTTCTAGGTGC

Teo TATGGCCTGCCGCACTCGGCAAAAGGTGGCGACTTTGCTGAGTGCCTTGGCGGCACTGGATAAAGCAAGTTTCTAGGTGC

3290 3300 3310 3320 3330 3340 3350 3360

| | | | | | | |

B73 CAAGGCATATTCATTTTTCCATATTTCATGTTATTGAGTATCATTTTTCCATA--------------CTCGATAAGTGTG

W22 CAAGGCATATTCATTTTTCCATATTTCATGTTATTGAGTATCATTTTTCCATATTCATTTTTCCATACTCGATAAGTGTG

Teo CAAGGCATATTCATTTTTCCATATTTCATGTTATTGAGTATCATTTTTCCATATTCATTTTTCCATACTCGATAAGTGTG

3370 3380 3390 3400 3410 3420 3430 3440

| | | | | | | |

B73 CAAGAAAAATTGATATGGCCTGCCGCACTCGGCAAAGGTGGTGACTTTGTTGAGTGCCTTGGCTCTGGCACTCGATAAAG

W22 CAAGAAAAATTGATATGGCCTGCCGCACTCGGCAAAGGTGGTGACTTTGTTGAGTGCCTTGGCTCTGGCACTCGATAAAG

Teo CAAGAAAAATTGATATGGCCTGCCGCACTCGGCAAAGGTGGTGACTTTGTTGAGTGCCTTGGCTCTGGCACTCGATAAAG

3450 3460 3470 3480 3490 3500 3510 3520

| | | | | | | |

B73 CAAGTTTCTAGGTGTCAAGGCATATCCATTTTTCCATATTCATGTTATTGAGTATCATTTTTCATTCATTTTTTCTATTA

W22 CAAGTTTCTAGGTGTCAAGGCATATCCATTATTCCATATTCATGTTATTGAGTATCATTTTTCATTCATTTTTTATATTA

Teo CAAGTTTCTAGGTGTCAAGGCATATCCATTTTTCCATATTCATGTTATTGAGTATCATTTTTCATTCATTTTTTCTATTA

3530 3540 3550 3560 3570 3580 3590 3600

| | | | | | | |

B73 TTCGAATGACTTGTAGTTCAGATTTGAATTATTCGAATACATTTAATTTAATAAAAAAAATTACTAGATATATCAAACAC

W22 TTCGAATGACTTGTAGTTCAGATTTGAATTATTCGAAGACATTTAATTTAATAAAAAAA-TTACTAGATATATCAAACAC

Teo TTCGAATGACTTGTAGTTCAGATTTGAATTATTCGAAGACATTTAATTTAATAAAAAAA-TTACTAGATATATCAAACAC

3610 3620 3630 3640 3650 3660 3670 3680

| | | | | | | |

B73 TTATAGAAATGTGCTAAAATTGAACATATAGTTCCATATACTCTCACAACAGCACTGTACACCATTTTCGGCGGCCCCTG

W22 TTATAGGAATGTGCTAAAATTGAACATATAGTTCCATATACTCT------------------------------------

Teo TTATAGAAATGTGCTAAAATTGAACATATAGTTCCATATACTCT------------------------------------

3690 3700 3710 3720 3730 3740 3750 3760

| | | | | | | |

B73 TTATTTTCGGCGGCTAGGGGGGGCCGCCGAAAATAGTGGGTTATTTTCGGCGGTCTGACACGACCGCCGAAAGTAGTGCT

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

3770 3780 3790 3800 3810 3820 3830 3840

| | | | | | | |

B73 ATTTTCGGCGGCCTCTGACACCAGCCGCCGAAAATAAGGCTATTTTCGGCGGCCTCTGACACCAGCCGCCGAAAATAAGG

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

3850 3860 3870 3880 3890 3900 3910 3920

| | | | | | | |

B73 CTATTTTCGGCGGCCTCTGACCTGGCCGCCGAAAATGAGAAATTTAAAACTGTCGGCCTTCTCCTATCTTCTTTCTCTCC

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

3930 3940 3950 3960 3970 3980 3990 4000

| | | | | | | |

B73 GCGCTCTCTCTCTCACCCGACGCCGCCGCGCTCTCTCTCTCACCCGACGCCGCGCCGTGTCTCTGCCCACGCCGCCGCGC

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4010 4020 4030 4040 4050 4060 4070 4080

| | | | | | | |

B73 CGCCGCCCGCGCCGCCGCCGCCGAAGGAAGCTGCGCGCACCGCCGTCCGCCGCCGTCCGTCCCCGCAGTGGTCGTCCGTC

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4090 4100 4110 4120 4130 4140 4150 4160

| | | | | | | |

B73 GGCCCGCCATTCCACCGCCGTCGTCGAGCCGCCGCATCGAGAGAGGTAATTTTTTTTAGTATTTTATTTCTTATTTTCGA

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4170 4180 4190 4200 4210 4220 4230 4240

| | | | | | | |

B73 CGGCTGTTATTTAGATGCCGCCGAAATGGCTTTTCTCCAACAGCAATCAGATTACATGGCTGCTTACAACGCACAGGCGC

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4250 4260 4270 4280 4290 4300 4310 4320

| | | | | | | |

B73 AACAAGCAATGAACGTGAGTGTCTTATTTATTCTCAACACGCTCGATATTGGTTCTATAACTAATATATTATATTTGCAA

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4330 4340 4350 4360 4370 4380 4390 4400

| | | | | | | |

B73 CAGTCTTGGTTTCCACAGCAGGCACAGCAGCAACCATTTGTTTTCCCTCAATTTCAGCCGCCGATGCCTCAGTGGGGATT

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4410 4420 4430 4440 4450 4460 4470 4480

| | | | | | | |

B73 ACATGCTCCGCCACCTCCACCTCCACCTCAGGTTATTCAATTTAGTTAAGACTTGTCGTTTGTATCAACCTAATTGTCAA

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4490 4500 4510 4520 4530 4540 4550 4560

| | | | | | | |

B73 ATCTTTACTAAACTTGATTTTGTAGGGATCCGGGGTCATGGGCCACAACACGCCACCACCGGTCATACCAGCACCAGGAG

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4570 4580 4590 4600 4610 4620 4630 4640

| | | | | | | |

B73 GAGCTTATGCAGGGGAGGGAGCTTATGCAGGAGAGGTTAGTTTGAGAAACAATTTGTTTGAATAGTCGTTATACATGTAA

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4650 4660 4670 4680 4690 4700 4710 4720

| | | | | | | |

B73 CTGTAGCCGTTATTAATGTCATCTTTTTTTTAGGATCCAAACCCGTTACACGACTTCGTCGACCAGTTATTGGCTTCTGG

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4730 4740 4750 4760 4770 4780 4790 4800

| | | | | | | |

B73 AGGTAGTGGACACAACTCCAACGACCCCAATGTGTGATTAAGTTAGCTTTGTGCCGCACTGTGTTGTAATGAACTATTTG

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4810 4820 4830 4840 4850 4860 4870 4880

| | | | | | | |

B73 TGGACTTTATGTTTGTATGGACTATTTGTGGACTTTATATTTGTATGGACTCAAGTTTTATGTTATGAACTCAAGTATGA

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4890 4900 4910 4920 4930 4940 4950 4960

| | | | | | | |

B73 ATTTTCTGTTATTGTTTGGAATTTCTGTGATTGTATGAATTTTGTATAAAATTCTGTTATTTTTCGTTTTTTTCAATTTT

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

4970 4980 4990 5000 5010 5020 5030 5040

| | | | | | | |

B73 TTTTGCTCTGAAATTAGTAATTTTCGGCGGCCCCCTGGCCGCCGAAAATAAGGGTTTTATTTTCGGCAGTTTTTTATTTT

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

5050 5060 5070 5080 5090 5100 5110 5120

| | | | | | | |

B73 CGGCGGCCGGCTCCCTGGCCGCCGAAAGTAAAGCCTTATTTTCGGCCAGTTTTTTTTGGCGGCCAGAAGCCGCCGAAAAT

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

5130 5140 5150 5160 5170 5180 5190 5200

| | | | | | | |

B73 AAGCCTTTTGCCGCCGAAAATAGCTTATTTTCGGCGGGAACTGGCTTATTTTCGGCGGCTTCTGGCCGCCGAAAATGACT

W22 --------------------------------------------------------------------------------

Teo --------------------------------------------------------------------------------

5210 5220 5230 5240 5250 5260 5270 5280

| | | | | | | |

B73 GTAGCTGTTGTAGTGTCTAGGAACATGATAGAAAAATGTTGGAGGAAAAAGAAAAAAATAAAGTCACTTTGTCGAGTGTC

W22 ------------------AGGAACACGATAGAAAAATGTTGGAGGAAAAAGAAAAAAATAAAGTCACTTTGCCGAGTGTC

Teo ------------------AGGAACACGATAGAAAAATGTTGGAGGAAAAAGAAAAAAATAAAGTCACTTTGCCGAGTGTC

5290 5300 5310 5320 5330 5340 5350 5360

| | | | | | | |

B73 AACAAAGGCACTCCCAAGCTGACCCTTTGCCGAGTGTCTGGAGGTAGCACTGGTGCCTTTGCCGAGTGCCAGCCTAAAAC

W22 AACAAAGGCACTCCCAAGCTGATCCTTTGCCGAGTGTCTGGAGGTAGCACTGGTGCCTTTGCCGAGTGCCAGCCTAAAAC

Teo AACAAAGGCACTCCCAAGCTGATCCTTTGCCGAGTGTCTGGAGGTAGCACTGGTGCCTTTGCCGAGTGCCAGCCTAAAAC

5370 5380 5390 5400 5410 5420 5430 5440

| | | | | | | |

B73 ACTTGGCAGACTTAACAATCGTGAGCTGTCGTCGGCTGCTTATGGCGTTTTTCCGAGTGACAGGTTTTGCGAGTGTCTAA

W22 ACTTGGCAGACTTAACAATCGTGAGCTGTCGTCGGCTGCTTATGGCGTTTTTCCGAGTGACAGGTTTTGCGAGTGTCTAA

Teo ACTTGGCAGACTTAACAATCGTGAGCTGTCGTCGGCTGCTTATGGCGTTTTTCCGAGTGACAGGTTTTGCGAGTGTCTAA

5450 5460 5470 5480 5490 5500 5510 5520

| | | | | | | |

B73 CACTAGGCAAAATAGGTGTCTGAATATGCCTCGGCAAACCACCTTTTTATCGAGTGTTTTGTTTTGCCGAGTGCGACACT

W22 CACTAGGCAAAATAGGTGTCTGGATATGGCTCAGCAAACCACCTTTTTGTCGAGTGTTTTGTTTTGCCGAGTGCGACACT

Teo CACTAGGCAAAATAGGTGTCTGGATATGGCTCGGCAAACCACCTTTTTGTCGAGTGTTTTGTTTTGCCGAGTGCGACACT

5530 5540 5550 5560 5570 5580 5590 5600

| | | | | | | |

B73 CGACTGACTTTGTCGAGTGCCTGACTTTTCATAATCGACAAATGTTTTAGAACTTTGCTAAATACCGCGTTTCGGATAGT

W22 CGGCTGACTTTGTCGAGTGCCTGACTTTTCATAATCGACAAATGTTTTAGAACTTTGCTAAATACCGCGTTTCGGATAGT

Teo CGGCTGACTTTGTCGAGTGCCTGACTTTTCATAATCGACAAATGTTTTAGAACTTTGCTAAATACCGCGTTTCGGATAGT

5610 5620 5630 5640 5650 5660 5670 5680

| | | | | | | |

B73 GGAAGGTCGTTTCTCTAGTGGCATAATGAAGTATATAGATCCAATTGGTCATGTGCATGCTGAGCCTTGTTGCGTGAAAC

W22 GGAAGGTCGTTTCTCTAGTGGCATAATGAAGTATATAGATCCAATTGGTCATGTGCATGCTGAGCCTTGTTGCGTGAAAC

Teo GGAAGGTCGTTTCTCTAGTGGCATAATGAAGTATATAGATCCAATTGGTCATGTGCATGCTGAGCCTTGTTGCGTGAAAC

5690 5700 5710 5720 5730 5740 5750 5760

| | | | | | | |

B73 TAAATGGCTTATTGTAATCTTTCACACCGGCCACCCTTGTGTTCCGTTCTCCACATTAATTTCATCGCAACTCGTAGTAA

W22 TAAATGGCTTATTGTAATCTTTCACACCGGCCACCCTTGTGTTCCGTTCTCCACATTAATTTCATCGCAACTCGTAGTAA

Teo TAAATGGCTTATTGTAATCTTTCACACCGGCCACCCTTGTGTTCCGTTCTCCACATTAATTTCATCGCAACTCGTAGTAA

5770 5780 5790 5800 5810 5820 5830 5840

| | | | | | | |

B73 ATACCAAAGGCCGAGGATAAAGGTAGCTAGAGTCGCAGAGATGATATACATCGATCAGTCAGTCAATCGCTGTGATGCTA

W22 ATACCAAAGGCCGAGGATAAAGGTAGCTAGAGTCGCAGAGATGATATACATCGATCAGTCAGTCAATCGCTGTGATGCTA

Teo ATACCAAAGGCCGAGGATAAAGGTAGCTAGAGTCGCAGAGATGATATATATCGATCAGTCAGTCAATCGCTGTGATGCTA

5850 5860 5870 5880 5890 5900 5910 5920

| | | | | | | |

B73 TGCATGCATATCTACTTGTGCAGTGTCGATACATATTTATACTATAGCTATATGAGCAGTGGGCGGATCTCTAGAAATTT

W22 TGCATGCATATCTACTTGTGCAGTGTCGATACATATTTATACTATAGCTATATGAGCAGTGGGCGGATCTCTAGAAATTT

Teo TGCATGCATATCTACTTGTGCAGTGTCGATACATATTTATACTATAGCTATATGAGCAGTGGGCGGATCTCTAGAAATTT

5930 5940 5950 5960 5970 5980 5990 6000

| | | | | | | |

B73 AGTGTGCGTTTTGTTGTAATGATAGAACGGGGTGGAATAGAACGATCACTTAAATAAGTTTGTTTGAGTTGAGACAGTAC

W22 AGTGTGCATTTTGTTGTAATGATAGAATGGGGTGGAATAGAACGATCACTTAAATAAGTTTGTTTGAGTTGAGACAGTAC

Teo AGTGTGCGTTTTGTTGTAATGATAGAACGGGGTGGAATAGAACGATCACTTAAATAAGTTTGTTTGAGTTGAGACAGTAC

6010 6020 6030 6040 6050 6060 6070 6080

| | | | | | | |

B73 TATCCTACTCCCGCAACCAAACACATCTTTAGAGAACCTCTAGCTAGAGGCTAGCTCTTCCCTTTTTCATTTTCATAAAA

W22 TATCCTACTCCCGCAACCAAACACATCTTTAGAGAACCTCTAGCTAGAGGCTAGCTCTTCCCTTTTTCATGTTCATAAAA

Teo TATCCTACCCCCGCAACCAAACACATCTTTAGAGAACCTCTAGCTAGAGGCTAGCTCTTCCCTTTTTCATTTTCATAAAA

6090 6100 6110 6120 6130 6140 6150 6160

| | | | | | | |

B73 GCTAGATAAAATGGGCAAAAGGAGGGGTCCTATATATTGTCGCGGAAGCAGTAGAGGGGGGGGGGGGGGGGG---CTTGA

W22 GCTAGATAAAATGGGCAAAAGGAGGGGTCCTATATATTGTCGCGGAAGCAGTAGAGGGGGGGGGGGGGGGGGGGGCTTGA

Teo GCTAGATAAAATGGGCAAAAGGAGGGGTCCTATATATTGTCGCGGAAGCAGAAGAGGGGGGGGGGGGGGGGGGGGCTTGA

6170 6180 6190 6200 6210 6220 6230 6240

| | | | | | | |

B73 GCGAGATCATCAT-------------------------------------------------------------------

W22 GCGAGATCATCATGGGTATGTTTGGTTCAGCTTTTTTCTGACCAGCTTTTCTGAAAATCTGGTTGTGGGGAGAATCTGGC

Teo GCGAGATCATCAT-------------------------------------------------------------------

6250 6260 6270 6280 6290 6300 6310 6320

| | | | | | | |

B73 --------------------------------------------------------------------------------

W22 TGTGGAGAGAATCTGAGTATTATTACGATTACGTGTGGAGGAAGATAAAGCTGTTCATAAGGCTCAGGATCTAGAAAGTG

Teo --------------------------------------------------------------------------------

6330 6340 6350 6360 6370 6380 6390 6400

| | | | | | | |

B73 --------------------------------------------------------------------------------

W22 ACGGATTCCTACTATTACAATGACTCAACCGATTATGTGTTTATATTGATTTTGGATGGTTTTTGCCGCAACGAATTTTA

Teo --------------------------------------------------------------------------------

6410 6420 6430 6440 6450 6460 6470 6480

| | | | | | | |

B73 --------------------------------------------------------------------------------

W22 TAGAAGCTGGCTGAAAAACTGAACGTTTGGCAGTCCGCAGCAGCTTTTGGTGGCCAGAAGCTGCCAGAAGCCGAAACAAA

Teo --------------------------------------------------------------------------------

6490 6500 6510 6520 6530 6540 6550 6560

| | | | | | | |

B73 ----------ATCTTTCGCAAGAGATCTTTGCCATCGCTGAAGTCTCTGAACGAAGTGGCAGTACTTTTTCTCCAGCTCC

W22 CATGGCCCATATCTTTCGCAAGAGATCTTTGCCATCGCTGAAGTCTCTGAACGAAGTGGCAGTACTTTTTCTCCAGCTCC

Teo ----------ATCTTTCGCAAGAGATCTTTGCCATCGCTGAAGTCTCTGAACGAAGTGGCAGTACTTTTTCTCCAGCTCC

6570 6580 6590 6600 6610 6620 6630 6640

| | | | | | | |

B73 AATGCCGTCTCCCCCTCTACTAGCCAAAGATAAAAAGGAGGTGTTTACTGGTCGATCTTTGCATTGCAAGCCCGCCGTGC

W22 AATGCCGTCTCCCCCTCTACTAGCCAAAGATAAAAAGGAGGTGTTTACTGGTCGATCTTTGCATTGCAAGCCCGCCGTGC

Teo AATGCCGTCTCCCCCTCTACTAGCCAAAGATAAAAAGGAGGTGTTTACTGGTCGATCTTTGCATTGCAAGCCCGCCGTGC

6650 6660 6670 6680 6690 6700 6710 6720

| | | | | | | |

B73 GAAAGTGGCTTCTGCATGAATTGTCACTATCTCCTTGCGTCTTTCTGGTCTGCAAAACTTTGTCCTAAATATATTCTTTC

W22 GAAAGTGGCTTCTGCATGAATTGTCACTATCTCCTTGCGTCTTTCTGGTCTGCAAAACTTTGTCCTAAATATATTCTTTC

Teo GAAAGTGGCTTCTGCATGAATTGTCACTATCTCCTTGCGTCTTTCTGGTCTGCAAAACTTTGTCCTAAATATATTCTTTC

6730 6740 6750 6760 6770 6780 6790 6800

| | | | | | | |

B73 AGCGTTTTGTGTTTGTGTCAGAGACGATGGACCATGCATACATACATGGAGCAGAAAATATATATTTATATATATAGCTG

W22 AGCGTTTTGTGTTTGTGTCAGAGACGATGGACCATGCATACATACATGGAGCAGAAAATATATATTTATATATATAGCTG

Teo AGCGTTTTGTGTTTGTGTCAGAGACGATGGACCATGCATACATACATGGAGCAGAAAATATATATTTATATATATAGCTG

6810 6820 6830 6840 6850 6860 6870 6880

| | | | | | | |

B73 CACGCGCGCTAATGGGTACGGTGATGAGTTCTCTACAAATCAGATCACACATCACATCGCACAATGTGTGTGTGTGTGCA

W22 CACGCGCGCTAATGGGTACGGTGATGAGTTCTCTACAAATCAGATCACACATCACATCGCACAAT---------------

Teo CACGCGCGCTAATGGGTACGGTGATGAGTTCTCTACAAATCAGATCACACATCACATCGCACAAT---------------

6890 6900 6910 6920 6930 6940 6950 6960

| | | | | | | |

B73 AGATATATGAAGTGTGACAGATTTAACTCATCGTCCCCTCATGGAGCACGTACACACAATCATTTGTAGGGCATACACTC

W22 ----ATATGAAGTGTGACAGATTTAACTCATCGTCCCCTCATGGAGCACGTACACACAATCATTTGTAGGGCATACACTC

Teo ----ATATGAAGTGTGACAGATTTAACTCATCGTCCCCTCATGGAGCACGTACACACAATCATTTGTAGGGCATACACTC

6970 6980 6990 7000 7010 7020 7030 7040

| | | | | | | |

B73 TCTCACTTGTTTAGCTAATACCATATGGGAGTTTGTGGAGCTCATGGGAGTTTGTGCGCACAAAAAAAGGACAGCGTGAG

W22 TCTCACTTGTTTAGCTAATACCATATGGGAGTTTGTGGAGCTCATGGGAGTTTGTGCGCACAAAAAAAGGACAGCGTGAG

Teo TCTCACTTGTT-AGCTAATACCATATGGGAGTTTGTGGAGCTCATGGGAGTTTGTGCGCACAAAAAA-GGACAGCGTGAG

7050 7060 7070 7080 7090 7100 7110 7120

| | | | | | | |

B73 GAATCAAACAAATTAAAGTAGGCCCTTTGCATTATTGTTTGTTCAGTTTGGGTGTGCACCAACAGTGTTTGTTCAGCAGC

W22 GAATCAAACAAATTAAAGTAGGCCCTTTGCATTATTGTTTGTTCAGTTTGGGTGTGCACCAACAGTGTTTGTTCAGCAGC

Teo GAATCAAACAAATTAAAGTAGGCCCTTTGCATTATTGTTTGTTCAGTTTGGGTGTGCACCAACAGTGTTTGTTCAGCAGC

7130 7140 7150 7160 7170 7180 7190 7200

| | | | | | | |

B73 AGAGCCCTGATAAAAGTCATGGAGCAAATAAACAGTTCCGCTGGCAGCCATATGCATGCAGGAAAAGAGAGAAGGCGTTC

W22 AGAGCCCTGATAAAAGTCATGGAGCAAATAAACAGTTCCGCTGGCAGCCATATGCATGCAGGAAAAGAGAGAAGGCGTTC

Teo AGA-CCCTGATAAAAGTCATGGAGCAAATAAACAGTTCCGCTGGCAGCCATATGCATGCAGGAAAAGAGAGAAGGCGTTC

7210 7220 7230 7240 7250 7260 7270 7280

| | | | | | | |

B73 GAGCATATGCATCTTTATTTTACTCCTCGTTCTTCTGCAAAAAACTATATGTAAAGAGGATTTTTTT-------------

W22 GAGCATATGCATCTTTATTTTACTCCTCGTTCTTCTGCAAAAAACTATATGTAAAGAGGATTTTTTTCACTACACGACGG

Teo GAGCATATGCATCTTTATTTTACTCCTCGTTCTTCTGCAAAAGCCTATATGTAAAGAGGATTTTTTT-------------

7290 7300 7310 7320 7330 7340 7350 7360

| | | | | | | |

B73 --------------------------------------------------------------------------------

W22 TTGATCTTTAGCGACCCTTATTTGGGACCGACGGTTGGTCGCTAAAAGTTATGGACCGATGGTTGGTCGCTAAATGCGTT

Teo --------------------------------------------------------------------------------

7370 7380 7390 7400 7410 7420 7430 7440

| | | | | | | |

B73 --------------------------------------------------------------------------------

W22 TATAGCGACGGTCTGTGGGTCGCTAAAAGTCTAGAAATTTAACAACTTATGGTCGGTCGCTATAGAGTTAAGTGTGTGAC

Teo --------------------------------------------------------------------------------

7450 7460 7470 7480 7490 7500 7510 7520

| | | | | | | |

B73 ---------------------------------------------CCCCTTGCTTCTGGTGTGCATCTTTTCATGCTATA

W22 TGATGGTGGA??????????GTAAGTGAACTCTCGTGTAGTGTTTCCCCTTGCTTCTGGTGTGCATCTTTTCATGCTATA

Teo --------------------------------------------TCCCCTTGCTTCTCGTGTGCACCTTTTCATGCTATA

7530 7540 7550 7560 7570 7580 7590 7600

| | | | | | | |

B73 TATATATATATACACTTTCACGGCGATAATTTAAGTACCGTAAGCAAATGCATACTGGATTTCTTCAATAATAAATAGAC

W22 TATATATATA--CACTTTCACGGCGATAATTTAAGTACCGTAAGCAAATGCATACTGGATTTCTTCAATAATAAATAGAC

Teo TATATA------CACTTTCACGGCGATAATTTAAGTACCGTAAGCAAATGCATACTGGATTTCTTCAATAATAAATAGAC

7610 7620 7630 7640 7650 7660 7670 7680

| | | | | | | |

B73 CAAATAATAATACTGGTGTCTCGTTTAATTTATGATCAATGTGTGCATGCAGGTGAGTGTCCCGGGTCACAGCATGCTGA

W22 CAAATAATAATACTGGTGTCTCGTTTAATTTATGATCAATGTGTGCATGCAGGTGAGTGTCCCGGGTCACAGCATGCTGA

Teo CTAATAATAATACTGGTGTCTCGTTTAATTTATGATCAATGTGTGCATGCAGGTGAGTGTCCCGGGTCACAGCATGCTGA

7690 7700 7710 7720 7730 7740 7750 7760

| | | | | | | |

B73 ACATGGTGACAGTCCGTTGTGGGCACTGCACTAGCCTGCTGTCGGTGAACTTGAGAGGACTCATCCAATCACTCCCTGTC

W22 ACATGGTGACAGTCCGTTGTGGGCACTGCACTAGCCTGCTGTCGGTGAACTTGAGAGGACTCATCCAATCACTCCCTGTC

Teo ACATGGTGACAGTCCGTTGTGGGCACTGCACTAGCCTGCTGTCGGTGAACTTGAGAGGACTCATCCAATCACTCCCTGTC

7770 7780 7790 7800 7810 7820 7830 7840

| | | | | | | |

B73 GTCCAGAATCACTACTCACAGGTTGCAATGAATTAATAGCTGCTAGCAACATCATATACTGATTCGTGCCGTGCAGTGCA

W22 GTCCAGAATCACTACTCACAGGTTGCAATGAATTAATAGCTGCTAGCAACATCATATACTGATTCGTGCCGTGCAGTGCA

Teo GTCCAGAATCACTACTCACAGGTTGCAATGAATTAATAGCTGCTAGCAACATCATA--CTGATTCGTGCCGTGCAGTGCA

7850 7860 7870 7880 7890 7900 7910 7920

| | | | | | | |

B73 TGATCTGCGCATGAAATATGAAGGACAACACGTACAATAAACCTCTTCTCTTCTAACAGAGCAGCTAATATAAACCTTTT

W22 TGATCTGCGCATGAAATATGAAGGACAACACGTACAATAAACCTCTTCTCTTCTAACAGAGCAGCTAATATAAACCTTTT

Teo TGATCTGCGCATGAAATATGAAGGACAACACGTACAATAAACCTCTTCTCTTCTAACAGAGCAGCTAATATAAACCTTTT

7930 7940 7950 7960 7970 7980 7990 8000

| | | | | | | |

B73 TTTTGTTTTTTGTCATATTGCTTCAAAACTGTAAC----CATGCATTCATGTCGACAAACGTTACTAACAACCTGTGTGA

W22 TTTTGTTTTTTGTCATATTGCTTCAAAACTGTAAC----CATGCATTCATGTCGACAAACGTTACTAACAACCTGTGTGA

Teo TTTTGTTTTTTGCCATATTGCTGCAAAACTGTAACGAACCATGCATTCATGTCGACAAACGTTACTAACAAACTGTGTGA

8010 8020 8030 8040 8050 8060 8070 8080

| | | | | | | |

B73 GTACTGTTACTAGCTTGTTATGCATTATTTACTGTAC----TATACTGCATTGTATCTCGCATGAAAGAGTTTAAAGCTT

W22 GTACTGTTACTAGCTTGTTATGCATTATTTACTGTAC----TATACTGCATTGTATCTCGCATGAAAGAGTTTAAAGCTT

Teo GTACTGTTACTAGCTTGTTATGCATTATTTACTGTACGTACTATACTGCAATGTATCTCGCATGAAAGAGTTTAAAGCTT

8090 8100 8110 8120 8130 8140 8150 8160

| | | | | | | |

B73 GCACACATGCATGC----TGCTAATTTGAACAAGATGCATCAGTTCTTCCTCACACCAAGAACTTGGGTCGATTACCAAA

W22 GCACACATGCATGC----TGCTAATTTGAACAAGATGCATCAGTTCTTCCTCACACCAAGAACTTGGGTCGATTACCAAA

Teo GCACACATGCATGCATGCTGCTAATTTGAACAAGATGCATCAGTTCTTCCTCACACCAAGAACTTGGGTCGATTACCAAA

8170 8180 8190 8200 8210 8220 8230 8240

| | | | | | | |

B73 GTGTGCATGCATGGAACATATACCAATAATAACATGATGATTATATTGTGTCAATTGGCTTCAGGAGCATTTCAAGGTCC

W22 GTGTGCATGCATGGAACATATACCAATAATAACATGATGATTATATTGTGTCAATTGGCTTCAGGAGCATTTCAAGGTCC

Teo GTGTGCATGCATGGAACATATACCAATAATAACATGATGATTATATTGTGTCAATTGGCTTCAGGAGCATTTCAAGGTCC

8250 8260 8270 8280 8290 8300 8310 8320

| | | | | | | |

B73 AAAATTTCAGCTTTACTGAGAACTATCCAGAGTATGCAGCACCACCTTCGTCTTCGAGATACCGCATGCCAACGATGTTG

W22 AAAATTTCAGCTTTACTGAGAACTATCCAGAGTATGCAGCACCACCTTCGTCTTCGAGATACCGCATGCCAACGATGTTG

Teo AAAATTTCAGCTTTACTGAGAACTATCCAGAGTATGCAGCACCACCTTCGTCTTCGAGATACCGCATGCCAACGATGTTG

8330 8340 8350 8360 8370 8380 8390 8400

| | | | | | | |

B73 TCAGCAAAAGGTGATCTGGATCATATGCTGCACGTCCGTGGTAAGCTAGATTGAATCATATATGTGTGGAAGTAAGTTTT

W22 TCAGCAAAAGGTGATCTGGATCATATGCTGCACGTCCGTGGTAAGCTAGATTGAATCATATATGTGTGGAAGTAAGTTTT

Teo TCAGCAAAAGGTGATCTGGATCATATGCTGCACGTGCGTGGTAAGCTAGATTGAATCATATATGTGTGGAAGTAAGTTTT

8410 8420 8430 8440 8450 8460 8470 8480

| | | | | | | |

B73 ATGAGAAACCTTTTTTT-CAGCATAAACACATGCGTGAAGAATCCAAAAGGAATTAATAAGAGCCCGTATTGAGTACTCC

W22 ATGAGAAACCTTTTTTT-CAGCATAAACACATGCGTGAAGAATCCAAAAGGAATTAATAAGAGCCCGTATTGAGTACTCC

Teo ATGAGAAACCTTTTTTTTCAGCATAAACACATGCGTGAAGAATCCAAAAGGAATTAATAAGAGCCCGTATTGAGTACTCC

8490 8500 8510 8520 8530 8540 8550 8560

| | | | | | | |

B73 CTCCATTCCAAAATGTTATTCGTTTTAGCTCTTGGTTTTTATAACTATATTAAAATATATGACAATGAATTTAGACACAT

W22 CTCCATTCCAAAATGTTATTCGTTTTAGCTCTTGGTTTTTATAACTATATTAAAATATATGACAATGAATTTAGACACAT

Teo CTCCATTCCAAAATGTCATTCGTTTTAGCTCTTGATTTTTATATCTATATTCAAATATATGACAATGAATTTAGACACAT

8570 8580 8590 8600 8610 8620 8630 8640

| | | | | | | |

B73 ATATAAAACATGTACATATATCAAGTATTATATGAATCTATTAATTAACTAAAATGAATGTTAATTTGGGACGGG---AT

W22 ATATAAAACATGTACATATATCAAGTATTATATGAATCTATTAATTAACTAAAATGAATGTTAATTTGGGACGGG---AT

Teo ATATAAAACATGTATATAAATCAATTATTATATGAATCCATTAATTAACTAAAACAAATGTTAATTTGGGACGGGGGAAT

8650 8660 8670 8680 8690 8700 8710 8720

| | | | | | | |

B73 ACTAAACTAAGGCTTATTA---AGCACAATATGATTTTTTTCAGAGAAATAATGATGTGGTTGCAAAAGCTAAGAATGAT

W22 ACTAAACTAAGGCTTATTA---AGCACAATATGATTTTTTTCAGAGAAATAATGATGTGGTTGCAAAAGCTAAGAATGAT

Teo ACTAAACTAAGGCTTATTATTAAGCACAATATGATTTTTT-CAGAGAAATAATGACGTGGTTGCAAAAACTAAGAATGAT

8730 8740 8750 8760 8770 8780 8790 8800

| | | | | | | |

B73 TCCTTTTAATATTATATTTGACTATAAATTATTTCTTGCAAATAATTCATCTCTCATTTACGTCTTGTTTTTGACAAAAA

W22 TCCTTTTAATATTATATTTGACTATAAATTATTTCTTGCAAATAATTCATCTCTCATTTACGTCTTGTTTTTGACAAAAA

Teo TCCTTTTAATATTATATTTGACTTTAAATTATTTCTTGCAAATAATTCATCTCTCGTTTACGTCTTGTTTTTGACAAAAA

8810 8820 8830 8840 8850 8860 8870 8880

| | | | | | | |

B73 GCAGAGGTAAAAGGTCATGTATATATACCATCATCACCTTGAAGTTCCTTGTGTACTATGCATGTGCTCATG-TTTTTTT

W22 GCAGAGGTAAAAGGTCATGTATATATACCATCATCACCTTGAAGTTCCTTGTGTACTATGCATGTGCTCATG-TTTTTTT

Teo GCATAGGTAAAAAGCCATGTATATATACCATCATCACCTTGAAGTTCCTTGTGTACTATGCATGTGCTCATGCTTTTTTT

8890 8900 8910 8920 8930 8940 8950 8960

| | | | | | | |

B73 TGCTTTGTTTCTACTCTTAATGTTGAAAATACAACTTAAAATATGAAAATACACTGCAAAATTGAATTTTCTTGCGAGTA

W22 TGCTTTGTTTCTACTCTTAATGTTGAAAATACAACTTAAAATATGAAAATACACTGCAAAATTGAATTTTCTTGCGAGTA

Teo TGCTTTGTTTCTACTCTTAAT---------------TAAAATATGAAAATACACTGCAAAATTGAATTTTCTTTCCACTA

8970 8980 8990 9000 9010 9020 9030 9040

| | | | | | | |

B73 AAATTAATACGATGAAGTTTAGTAACCCTATTATGAAGTGTTGGGTAAAATTCATATAGTTTCCAAAAGTGAGTAAACCA

W22 AAATTAATACGATGAAGTTTAGTAACCCTATTATGAAGTGTTGGGTAAAATTCATATAGTTTCCAAAAGTGAGTAAACCA

Teo AAATTAATACAATGAAGTTTAGTAACCCTATTATGAAGTGTTGGGTAAAATTCATATAGTTTCCAAAAGTGAGTAAACCA

9050 9060 9070 9080 9090 9100 9110 9120

| | | | | | | |

B73 AACATAGATAAATGTGTGCTTATAGTATTCTCTAAAAATAAGACTAGCTAATAAATATTCCAAATTCATATGTATACTTC

W22 AACATAGATAAATGTGTGCTTATAGTATTCTCTAAAAATAAGACTAGCTAATAAATATTCCAAATTCATATGTATACTTC

Teo AACATAGATAAATGTGT-CTTATAGTATTCTCTGAAAATAAGACTAGCCGATAAATATTCCGAATTCATATGTATACTTC

9130 9140 9150 9160 9170 9180 9190 9200

| | | | | | | |

B73 ATTGCTTCATGCTAGCTCTTCTAATTCTGAAACCGTTTTTAAATATAAACCGAACGGTTTTCCCAAATGTTCAGAATCTC

W22 ATTGCTTCATGCTAGCTCTTCTAATTCTGAAACCGTTTTTAAATATAAACCGAACGGTTTTCCCAAATGTTCAGAATCTC

Teo ATTACTTCATGCTAGCTCTTCTAATTCTGAAACCGTTGTTTAATATAAACCGAACGGTTTTCCCAAATGTTTAGAATTTT

9210 9220 9230 9240 9250 9260 9270 9280

| | | | | | | |

B73 CCTTGTTGGTTGGTGTGTGAGGTGTTCATGATGGGGTTTCATGTGGCCTTCACAGTACACTTTGCAATTGTATATTAACT

W22 CCTTGTTGGTTGGTGTGTGAGGTGTTCATGATGGGGTTTCATGTGGCCTTCACAGTACACTTTGCAATTGTATATTAACT

Teo CCTTGTTGGTTGGTGTGTGAGGTGTTCATGATGGGGTTTCATGTGTCCTTCACAGTACACTTTGCAATTGTATATTAACT

9290 9300 9310 9320 9330 9340 9350 9360

| | | | | | | |

B73 ATAATTCATCCATACAAGAGTGTTTTCAGCAAAGAGGATTTTGTAAAATATGTATATCTATGGTGTGTATGTCCTAATAA

W22 ATAATTCATCCATACAAGAGTGTTTTCAGCAAAGAGGATTTTGTAAAATATGTATATCTATGGTGTGTATGTCCTAATAA

Teo ATTATTCAACCATACAAGAGTGTTTTCAGCAAAGAGGATTTTGTAAAATATGTATATCTATGGTGTGTATGTCCTAATAA

9370 9380 9390 9400 9410 9420 9430 9440

| | | | | | | |

B73 CTTCATGTGTCTGGTCTTTGTAATAAACAACAGCTCCAGAGAAGAGGCAGCGTGTTCCTTCAGCATATAACAGATTTATT

W22 CTTCATGTGTCTGGTCTTTGTAATAAACAACAGCTCCAGAGAAGAGGCAGCGTGTTCCTTCAGCATATAACAGATTTATT

Teo CTTCATGTGTCTGGTCTTTGTAATAAACAACAGCTCCAGAGAAGAGGCAGCGTGTTCCTTCAGCATATAACAGATTTATT

9450 9460 9470 9480 9490 9500 9510 9520

| | | | | | | |

B73 AAGTGAGTTGGACTAAGTCTAAGTTCATTT-CTATTTTACATTCTCTCAAATTTAAATTGTAGACCGCCATTTTAGTTTT

W22 AAGTGAGTTGGACTAAGTCTAAGTTCATTT-CTATTTTACATTCTCTCAAATTTAAATTGTAGACCGCCATTTTAGTTTT

Teo AAGTGAGTTGGAC-CAGTCTAAGTTCATTTTCTATTTTACATTCTCTCAAATTTAAATTGTAGACC---ATTTTAGTTTT

9530 9540 9550 9560 9570 9580 9590 9600

| | | | | | | |

B73 GT-TTAAGTCAAACTTATTTAACATTAACCAGATTTATGAAAAAAGTATGTTAGCATCTACAATCAAACAAATGCACTAT

W22 GT-TTAAGTCAAACTTATTTAACATTAACCAGATTTATGAAAAAAGTATGTTAGCATCTACAATCAAACAAATGCACTAT

Teo GTCTTAAGTCAAACTTATTTAACATTAACCAGATTTATGAAAAAAGTATGTTAGCATCTACAATCAAACAAATGCACTAT

9610 9620 9630 9640 9650 9660 9670 9680

| | | | | | | |

B73 TAAAATACATTCCATGCTGTTACTAATGGATATCGTTTTATGAAAATATATTATATGATGTATCTAGTGAAATTTGTTTG

W22 TAAAATACATTCCATGCTGTTACTAATGGATATCGTTTTATGAAAATATATTATATGATGTATCTAGTGAAATTTGTTTG

Teo TAAAATATATTCCATGTTGTTACTAATGGATATCGTTTTATCATAATATATTATATGATGTATCTAGTGAAATTTGTTTG

9690 9700 9710 9720 9730 9740 9750 9760

| | | | | | | |

B73 ATACTATAGATGATTGTGCGATTTTTTTATAAATTTGATCAAAGTTAGAGAATCTTTGAGTTAGGACAAAACTAAAACAA

W22 ATACTATAGATGATTGTGCGATTTTTTTATAAATTTGATCAAAGTTAGAGAATCTTTGAGTTAGGACAAAACTAAAACAA

Teo ATATTATAGCTGATTGTGCAGTTTTTT-ATAAATTTGATCAAAGTTACAGAATCTTTGAGTTAGGACAAAACTAAAGCAA

9770 9780 9790 9800 9810 9820 9830 9840

| | | | | | | |

B73 TATATATTTTGGAATGAAGGGTGTACGTATGTTGTAGTATGGTTTATGTCTAGGTATAAACAATGTAGTTTTATATAAAC

W22 TATATATTTTGGAATGAAGGGTGTACGTATGTTGTAGTATGGTTTATGTCTAGGTATAAACAATGTAGTTTTATATAAAC

Teo TATATACTTTGGAATGAAGGGTGTACGTATGTTGTAGTATGGTTTATGTCTAGGTATAAACAGTCTAGTTTTATATAAAC

9850 9860 9870 9880 9890 9900 9910 9920

| | | | | | | |

B73 TAATATTGAAATGGTGCATGCATTGTCTTAGGGAAGAGATACGAAGGATTAAAGCAAACAACCCGGACATAAGCCATAGG

W22 TAATATTGAAATGGTGCATGCATTGTCTTAGGGAAGAGATACGAAGGATTAAAGCAAACAACCCGGACATAAGCCATAGG

Teo TAATATTGAAATGGTGCATGCATTGTCTTAGGGAAGAGATACGAAGGATTAAAGCAAACAACCCGGACATAAGCCATAGG

9930 9940 9950 9960 9970 9980 9990 10000

| | | | | | | |

B73 GAAGCTTTCAGCACTGCAGCAAAGAAT--AAGTACATGGTTTACATAGAACAAGTGTTGTCCTATACTCAGGATA-----

W22 GAAGCTTTCAGCACTGCAGCAAAGAATGTAAGTACATGGTTTACATAGAACAAGTGTTGTCCTATACTCAGGATA-----

Teo GAAGCCTTTAGCACTGCAGCAAAGAATGTAAGTACATGGTTTGCAAATAACAAGTGTTGTCCTATACTCAGGATATATTG

10010 10020 10030 10040 10050 10060 10070 10080

| | | | | | | |

B73 ----------------ATTATCCCATGAAAATAAATTAGATGGAAAAAAATGCAAGGAAATTGATATTGAACTTTATTAA

W22 ----------------ATTATCCCATGAAAATAAATTAGATGGAAAAAAATGCAAGGAAATTGATATTGAACTTTATTAA

Teo TATGTATGTAGGGATAATTATCCCATGAAAATTAATTAGATGGAAAAA--TGCAAGGAAATTGATATTGAA-TTTACTAA

10090 10100 10110 10120 10130 10140 10150 10160

| | | | | | | |

B73 TAAAGATGTCTTAAAATTAATCTCATTTATCTCAAACTTTTTTAAAAA-TGTGAACAATGTTTTTTGATAATCACAAAAT

W22 TAAAGATGTCTTAAAATTAATCTCATTTATCTCAAACTTTTTTAAAAA-TGTGAACAATGTTTTTTGATAATCACAAAAT

Teo TAAAGATGTCTTAAAATTAATCTCATTTATCTCAAACTTTTTTAAAAAATGTGAACAATGTTTTTTGATAATCACAAAAT

10170 10180 10190 10200 10210 10220 10230 10240

| | | | | | | |

B73 ACTTACCAAACATTGCTTTGTTGATTAATTGTTCATCTATAACTCCTTGATACTCTCATACCATGATTTCCTGAACTATA

W22 ACTTACCAAACATTGCTTTGTTGATTAATTGTTCATCTATAACTCCTTGATACTCTCATACCATGATTTCCTGAACTATA

Teo ACTTACCAAACATTGCTTTGTTGATTAATTGTTCATCTATAACTCCTTGATACTCTCATACCGTGATTTCCTGA-CTATA

10250 10260 10270 10280 10290 10300 10310 10320

| | | | | | | |

B73 TTAATTTATACCATAAACATATTGTACAATCTAGATACCATATACAAACTACTTGGTAAGAACACATTTTGTTATCCAAC

W22 TTAATTTATACCATAAACATATTGTACAATCTAGATACCATATACAAACTACTTGGTAAGAACACATTTTGTTATCCAAC

Teo TTAATCTATATCATAAACATATTGTACAAGCTAGATACCATATACAAACTACTTGGTAAGAACACATTTTGTTATCCAAC

10330 10340 10350 10360 10370 10380 10390 10400

| | | | | | | |

B73 AAAATTTGTTCAAATCATCATTGTTTGTATTAATTTCCTTTCTTCAAAGAGTTAGTTAAGAAAAACAAGAACATATATGA

W22 AAAATTTGTTCAAATCATCATTGTTTGTATTAATTTCCTTTCTTCAAAGAGTTAGTTAAGAAAAACAAGAACATATATGA

Teo AAAATTTTTTCAAATCATCATTGTTTGTATTAATTTCCTTTCTTCAAAGAGTTAGTTAAGAAAAACAAGAACATATATGA

10410 10420 10430 10440 10450 10460 10470 10480

| | | | | | | |

B73 ATTTCTTAAAAACAATGTATAAAATGCACCCAATGCATCCATATTGATTTTGTTTTCCATTTCAGTGGGCACATTTTCCG

W22 ATTTCTTAAAAACAATGTATAAAATGCACCCAATGCATCCATATTGATTTTGTTTTCCATTTCAGTGGGCACATTTTCCG

Teo ATTTCTTAAAAACAATGTATAAAATGCACCCAATGCATCCATATTGATTTTGTTTTCCATTTCAGTGGGCACATTTTCCG

10490 10500 10510 10520 10530 10540 10550 10560

| | | | | | | |

B73 AACATTCATTTTGGACTCGGGCCCTATGAAAGCAGCAACAAGCTTGATGAGACCATTGGTGCAACGGGTCATCCTCGGAA

W22 AACATTCATTTTGGACTCGGGCCCTATGAAAGCAGCAACAAGCTTGATGAGACCATTGGTGCAACGGGTCATCCTCGGAA

Teo AACATTCATTTTGGACTCGGGCCCTATGAAAGTAGCAACAAGCTTGATGAGACCATTGGTGCAACGGGTCATCCTCGGAA

10570 10580 10590 10600 10610 10620 10630 10640

| | | | | | | |

B73 AATTCAAGATCCCTACTAAGACATATATCGACCTCGTTCTTTGTTTTCCTATTGTAAGTAATTTTAGATAAAAAA-CTAT

W22 AATTCAAGATCCCTACTAAGACATATATCGACCTCGTTCTTTGTTTTCCTATTGTAAGTAATTTTAGATAAAAAA-CTAT

Teo AATTCAAGATCCCTACTAAGACCTATATCGACATCGTTCTTTGTTTTCCTATTGTAAGTAATTTTAGATAAAAAAACTAT

10650 10660 10670

| | |

B73 CTTAGTTCTGAACTAGTTTGTGAGTGTCGTACATATA

W22 CTTAGTTCTGAACTAGTTTGTGAGTGTCGTACATATA

Teo CTTAGTTCCGAACTAGTTTGTGAGTGTCGTGTATATA

Figure S7. Alignment of GRMZM2G085873 sequences from B73, W22 and teosinte. All six

exons are highlighted in grey. Start codon is highlighted in green while stop codon is

highlighted in red. B73_Spm insertion is highlighted in yellow while W22_Spm insertion is

highlighted in blue. Note that W22_Spm insertion is not completely sequenced due to the

challenge in sequencing through terminal inverted repeats of a transposable element without an

appropriate W22 bacterial artificial chromosome.

Figure S8. Phylogenetic tree for YABBY gene family. Posterior probabilities are labelled on

each node. ZmYAB2.1 falls into YAB2 clade and is highlighted in bold.

Figure S9. Linkage disequilibrium (LD) plots of SNPs from ZmYAB2.1 exon 1 region, 34 kb

upstream region, 68 kb upstream region, and five other randomly selected genomic regions from

publicly available maize 55k SNP Chip dataset (Cook et al. 2012). Note that only SNPs from

maize landraces listed in Table S2 are used here. R2 is shown on the bottom left while P-values

are shown on the top right. Both R2 and P-values use the same color scale, and P-value of 1.5e-5

corresponds to Bonferroni-corrected P-value of 0.05. There is little to no LD between

ZmYAB2.1 exon 1 region and 34 kb upstream region, suggesting that there is no power to detect

any signature of selection on the upstream regulatory region of ZmYAB2.1.

Table S1. Markers used in fine-mapping etb1.2.

Marker Type AGP_v2

Position Polymorphism Primers (5’-3’)

umc2047 SSR 258383492 Small (T); Large (M) (F) GACAGACATTCCTCGCTACCTGAT

(R) CTGCTAGCTACCAAACATTCCGAT

GBS_81 GBS 258383884 SNP -

GBS_82 GBS 258383890 SNP -

GBS_83 GBS 258383911 SNP -

GBS_84 GBS 258386943 SNP -

GBS_85 GBS 258387379 SNP -

GBS_86 GBS 258387646 SNP -

GBS_87 GBS 258397535 SNP -

GBS_88 GBS 258427527 SNP -

GBS_89 GBS 258437034 SNP -

GBS_90 GBS 258437091 SNP -

GBS_91 GBS 258441125 SNP -

GBS_92 GBS 258441270 SNP -

GBS_93 GBS 258442518 SNP -

GBS_94 GBS 258442582 SNP -

GBS_95 GBS 258442796 SNP -

GBS_96 GBS 258447918 SNP -

GBS_97 GBS 258447921 SNP -

GBS_98 GBS 258448383 SNP -

GBS_99 GBS 258448441 SNP -

GBS_100 GBS 258448583 SNP -

GBS_101 GBS 258521637 SNP -

GBS_102 GBS 258551646 SNP -

GBS_103 GBS 258552796 SNP -

GBS_104 GBS 258553443 SNP -

GBS_105 GBS 258651730 SNP -

GBS_106 GBS 258651762 SNP -

GBS_107 GBS 258655425 SNP -

GBS_108 GBS 258655426 SNP -

GBS_109 GBS 258656283 SNP -

GBS_110 GBS 258656358 SNP -

GBS_111 GBS 258656778 SNP -

GBS_112 GBS 258657130 SNP -

GBS_113 GBS 258657467 SNP -

GBS_114 GBS 258657767 SNP -

GBS_115 GBS 258673732 SNP -

GBS_116 GBS 258674017 SNP -

GBS_117 GBS 258681240 SNP -

GBS_118 GBS 258691831 SNP -

GBS_119 GBS 258796234 SNP -

GBS_120 GBS 258796310 SNP -

LEK025_026 Indel 258799855 321 (T); 326 (M) (F) GTACCTCCGACGACTCGAAA

(R) CATAGAGCTGGTGGGGAGTG

GBS_121 GBS 258800041 SNP -

GBS_122 GBS 258800198 SNP -

GBS_123 GBS 258800389 SNP -

GBS_124 GBS 258803723 SNP -

GBS_125 GBS 258803752 SNP -

GBS_126 GBS 258808508 SNP -

GBS_127 GBS 258875029 SNP -

GBS_128 GBS 258878686 SNP -

GBS_129 GBS 258878734 SNP -

LEK037_038

(SBM01) SBM 259017755 SNP + Indel

(F) GGCCTAGACACTGCCTTCTG

(R) TTGGCTGAATCCTTCTCGTT

GBS_130 GBS 259017915 SNP -

GBS_131 GBS 259023565 SNP -

GBS_132 GBS 259023731 SNP -

GBS_133 GBS 259024225 SNP -

GBS_134 GBS 259174085 SNP -

GBS_135 GBS 259174254 SNP -

GBS_136 GBS 259175533 SNP -

GBS_137 GBS 259175601 SNP -

GBS_138 GBS 259175680 SNP -

GBS_139 GBS 259180657 SNP -

GBS_140 GBS 259181338 SNP -

GBS_141 GBS 259210821 SNP -

GBS_142 GBS 259212357 SNP -

GBS_143 GBS 259212464 SNP -

GBS_144 GBS 259214758 SNP -

GBS_145 GBS 259215322 SNP -

GBS_146 GBS 259215767 SNP -

GBS_147 GBS 259217246 SNP -

GBS_148 GBS 259217414 SNP -

GBS_149 GBS 259217670 SNP -

GBS_150 GBS 259217673 SNP -

GBS_151 GBS 259219693 SNP -

GBS_152 GBS 259219993 SNP -

GBS_153 GBS 259220072 SNP -

GBS_154 GBS 259220966 SNP -

GBS_155 GBS 259220974 SNP -

GBS_156 GBS 259221159 SNP -

GBS_157 GBS 259221612 SNP -

GBS_158 GBS 259221873 SNP -

GBS_159 GBS 259222739 SNP -

GBS_160 GBS 259223303 SNP -

GBS_161 GBS 259223380 SNP -

GBS_162 GBS 259223619 SNP -

GBS_163 GBS 259225324 SNP -

GBS_164 GBS 259225871 SNP -

GBS_165 GBS 259235250 SNP -

GBS_166 GBS 259235364 SNP -

GBS_167 GBS 259239221 SNP -

GBS_168 GBS 259272819 SNP -

GBS_169 GBS 259272837 SNP -

GBS_170 GBS 259274478 SNP -

GBS_171 GBS 259301362 SNP -

GBS_172 GBS 259301727 SNP -

GBS_173 GBS 259304663 SNP -

GBS_174 GBS 259304819 SNP -

umc1298 SSR 259304887 177 (T); 179 (M) (F) AGGACAAGAAAAAGAAGAAGCACG

(R) AGCTGAACAAAATAAACGGAACGA

GBS_175 GBS 259304921 SNP -

GBS_176 GBS 259304941 SNP -

GBS_177 GBS 259372795 SNP -

GBS_178 GBS 259379916 SNP -

GBS_179 GBS 259407119 SNP -

GBS_180 GBS 259407231 SNP -

GBS_181 GBS 259408089 SNP -

GBS_182 GBS 259532659 SNP -

GBS_183 GBS 259538298 SNP -

GBS_184 GBS 259701285 SNP -

LEK055_056

(SBM02) SBM 259714872 SNP + Indel

(F) GGCATAGCTCGAGGAACAAC

(R) TGTAGAAGGGGATTTGGCTG

GBS_185 GBS 259746774 SNP -

GBS_186 GBS 259771415 SNP -

GBS_187 GBS 259772662 SNP -

GBS_188 GBS 259772740 SNP -

GBS_189 GBS 259778419 SNP -

GBS_190 GBS 259816063 SNP -

GBS_191 GBS 259817618 SNP -

GBS_192 GBS 259825061 SNP -

GBS_193 GBS 259930666 SNP -

GBS_194 GBS 259937100 SNP -

GBS_195 GBS 259937306 SNP -

GBS_196 GBS 259937627 SNP -

GBS_197 GBS 259948037 SNP -

GBS_198 GBS 259948106 SNP -

GBS_199 GBS 259949194 SNP -

GBS_200 GBS 259949290 SNP -

GBS_201 GBS 260142416 SNP -

GBS_202 GBS 260143885 SNP -

GBS_203 GBS 260144220 SNP -

GBS_204 GBS 260144338 SNP -

GBS_205 GBS 260150212 SNP -

GBS_206 GBS 260150230 SNP -

GBS_207 GBS 260150257 SNP -

GBS_208 GBS 260150325 SNP -

GBS_209 GBS 260338377 SNP -

GBS_210 GBS 260338525 SNP -

GBS_211 GBS 260339199 SNP -

LEK067_068 Indel 260366895 130 (M); 138 (T) (F) GCATCTTGTTCAAATTAGCAGC

(R) GTGAGTACTGTTACTAGCTTGTTATGC

CJ071_021

(SBM03) SBM 260383398 SNP + Indel

(F) TCTGGTTATGCGAAAGAAGC

(R) TCTGCTGGTTGATGGACTAC

CJ136_118

(SBM04) SBM 260448142 SNP + Indel

(F) TGTCTTGAGWGGTTCTAGTG

(R) AGTTCATTCACCTGTGTTGG

CJ004_005

(SBM05) SBM 260448854 SNP + Indel

(F) GTTCATTCTATTTCCAGGAC

(R) CCAGGCACCTGATGGAATC

GBS_212 GBS 260514553 SNP -

GBS_213 GBS 260514645 SNP -

GBS_214 GBS 260517297 SNP -

GBS_215 GBS 260517356 SNP -

GBS_216 GBS 260522263 SNP -

GBS_217 GBS 260621279 SNP -

GBS_218 GBS 260655699 SNP -

GBS_219 GBS 260655766 SNP -

GBS_220 GBS 260655851 SNP -

LEK092_093 Indel 260656326 211 (T); 217 (M) (F) CTGATGCGTAGCGTATATCGAG

(R) AGGCCTCCTTTGATCCCAA

LEK095_096

(SBM06) SBM 260679274 SNP + Indel

(F) TTGCTGATATTGTTCGCTCG

(R) CAACTCGATGGAAAGCCATT

LEK097_098

(SBM07) SBM 260684606 SNP + Indel

(F) ACATCATCCCCGTGAAAGAG

(R) TCAAGTGGTTCTCCCAGTCC

GBS_221 GBS 260711087 SNP -

GBS_222 GBS 260745399 SNP -

GBS_223 GBS 260746476 SNP -

GBS_224 GBS 260771404 SNP -

umc1411 SSR - Small (T); Large (M) (F) CGATAATGAAGATGAGCTCCGTG

(R) CTAGCCGGTTGTTGTTGCCT

Table S2. Primers used for sequencing ZmYAB2.1

Primer Sequence (5’ – 3’) Location Direction

CJ021 TCTGCTGGTTGATGGACTAC 5’ UTR Forward

CJ074 ATGTCGTCGGCGGCCCAGAT Exon 1 Forward

CJ022 AGACGAAGCTAGTCAGCAAG Intron 1 Forward

CJ115 GTTCTCAAAGCATTCTCGCC Intron 1 Forward

CJ071 TCTGGTTATGCGAAAGAAGC Intron 1 Reverse

CJ072 TCGATCAAGAAGGTAACGAG Intron 1 Reverse

CJ024 CCAGGTCATCGGCAGTACAC Intron 1 Reverse

CJ025 CCACAAACCTTAGTGACAATG Intron 1 Forward

CJ026 CCGCTATGCTAAAACATTATTC Intron 1 Forward

CJ116 GCACAAGAGAACAACGATAG Intron 1 Reverse

CJ114 AACCTCATGTATTGTCTCGC Intron 1 Forward

CJ027 GGACTAATAAGAGCTGGACG Intron 1 Reverse

CJ028 AAGCATATCAGTCCTGGTTTG Intron 1 Reverse

CJ096 TTCGAATGACTTGTAGTTCAG Intron 1 Forward

CJ131 CCATATACTCTCACAACAGC Intron 1 Forward

CJ135 ACCTCCACCTCAGGTTATTC Intron 1 Forward

CJ168 CGCACTGTGTTGTAATGAAC Intron 1 Forward

CJ169 AAAGCCTTATTTTCGGCCAG Intron 1 Forward

CJ133 CGCCGAAAATAAGCCAGTTC Intron 1 Reverse

CJ130 TTGTTGACACTCGGCAAAGT Intron 1 Reverse

CJ132 AGTGCCTTTGTTGACACTCG Intron 1 Reverse

CJ129 TAACAATCGTGAGCTGTCGT Intron 1 Forward

CJ127 ACACTCGACAAAAAGGTGGT Intron 1 Reverse

CJ103 GCGTYTCGGATAGTGGAAGG Intron 1 Forward

CJ109 AAACSACCTTCCACTATCCG Intron 1 Reverse

CJ104 GTTGCRTGAAACTAAATGGC Intron 1 Forward

CJ126 TTAATGTGGAGAACGGAACA Intron 1 Reverse

CJ125 TACTGCCACTTCGTTCAGAG Intron 1 Reverse

CJ120 GTTTACTGGTCGATCTTTGC Intron 1 Forward

CJ107 TGGAGCACGTACACACAATC Intron 1 Forward

CJ106 TGATTGTGTGTACGTGCTCC Intron 1 Reverse

CJ196 AGATCAACCGTCGTGTAGTG Intron 1 Reverse

CJ198 CCACCATCAGTCACACACTT Intron 1 Forward

CJ105 TTTGATTCCTCACGCTGTCC Intron 1 Reverse

CJ101 TTTGCAGAAGAACGAGGAGT Intron 1 Reverse

CJ057 AACATGGTGACAGTCCGTTG Exon 2 Forward

CJ058 GTGAGTAGTGATTCTGGACG Exon 2 Reverse

CJ085 TCAAGGTCCAAAATTTCAGC Exon 3 Forward

CJ055 ACGTGCAGCATATGATCCAG Exon 3 Reverse

CJ054 GTTATATGCTGAAGGAACACG Exon 4 Reverse

CJ075 CTTATGTCCGGGTTGTTTGC Exon 5 Reverse

CJ061 GTTGCACCAATGGTCTCATC Exon 6 Reverse

CJ079 TCATTCGTGATACTAGGAGC 3’ UTR Reverse

Table S3. Samples used for DNA sequence analysis and selection tests

Line DNA Prep Type Race/Species Country

MR01 BKN_023 Landrace Inbred Araguito Venezuela

MR02 BKN_014 Landrace Inbred Assiniboine USA

MR05 BKN_019 Landrace Inbred Cateto Bolivia

MR06 BKN_033 Landrace Inbred Chapalote Mexico

MR08 BKN_034 Landrace Inbred Costeno Venezuela

MR09 BKN_032 Landrace Inbred Cravo Riogranense Brazil

MR12 BKN_015 Landrace Inbred Havasupai USA

MR13 BKN_040.1 Landrace Inbred Hickory King USA

MR14 BKN_016 Landrace Inbred Longfellow Flint Canada

MR15 BKN_028 Landrace Inbred Palomero De Jalisco Mexico

MR17 BKN_026 Landrace Inbred Pisankalla Bolivia

MR18 BKN_022 Landrace Inbred Reventador Mexico

MR19 BKN_017 Landrace Inbred Santa Domingo USA

MR20 BKN_018 Landrace Inbred Shoe Peg USA

MR21 BKN_035 Landrace Inbred Tabloncillo Mexico

MR22 BKN_025 Landrace Inbred Tuxpeno Mexico

MR23 BKN_030 Landrace Inbred Zapalote Chico Mexico

MR24 BKN_009 Landrace Inbred Chullpi Peru

MR25 BKN_010 Landrace Inbred Pororo Bolivia

TIL01 TIP-521.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL03 TIP-523.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL04 TIP-454.5 Teosinte Inbred Zea mays ssp. parviglumis -

TIL05 TIP-458.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL06 TIP-496.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL07 TIP-466.5 Teosinte Inbred Zea mays ssp. parviglumis -

TIL08 TIP-529.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL09 TIP-503.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL10 TIP-485.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL11 TIP-534.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL12 TIP-508.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL14 TIP-498.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL16 TIP-469.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL17 TIP-512.1 Teosinte Inbred Zea mays ssp. parviglumis -

TIL18 TIP-475.5 Teosinte Inbred Zea mays ssp. mexicana -

TIL25 TIP-489.1 Teosinte Inbred Zea mays ssp. mexicana -

M006 Q171 Open pollinated Zea diploperennis -

Table S4. Samples used for surveying allele distribution

Accession Stock Subspecies Type Racename Country Stateprovince Population Latitude Longitude Altitude

(ft)

W22

Spm

B73

Spm

Mo17

82bp

Ki3

2bp

N/A 38-11 (PURD-AG) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A A158 (MINN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A A441-5 (NATAL) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A A6 (PHI) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A A619 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A B37 (ISU) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A B73 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A B97 (ISU) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CM105 (AGCAN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CM174 (AGCAN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A CM37 (BB) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A CML103 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A CML228 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CML247 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A CML277 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A CML322 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A CML333 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CML52 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CML69 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A H99 (PURD-AG) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A Hp301 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A I29 (PURD-BOT) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Ia2132 (UIUC-NR) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Il14H (UIUC-NR) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K13 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K133 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K141 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K187 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K39 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K42 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K50 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K55 (ARS-MO) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A Ki11 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A Ki3 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- +/+

N/A Ky21 (KY) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A M162W mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A M37W mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A Mo17 (ARS-MO) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A MO18W mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A MS153 (MICH) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A MS71 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A N192 (NEBR) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A NC350 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A NC358 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A Oh43 (OHW) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Oh7B (OHW) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A P39 (PURD-AG) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Q229 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A SC55 (SC) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Sg18 (PURD-BOT) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A T232 (BB) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A T8 (TENN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A Tx303 mays Inbred N/A N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+

N/A Tzi8 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A Va26 (VATECH) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A W182B (WISC) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A W22 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A Z134 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL01-S8 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL02-S3 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL03-S8 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL04-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL05-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL06-S6 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL07-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL08-S8 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL09-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- +/+

N/A TIL10-S6 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL11-S9 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL12-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL14-S6 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL15-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL16-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL17-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL18-S4 mexicana Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL25-S5 mexicana Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

8750 8750 (CIMMYT) mexicana OP Chalco Mexico Mexico Ozumba 19.05 N 98.82 W 2340 -/- -/- -/- -/-

8769 8769 (CIMMYT) mexicana OP Chalco Mexico Distrito

Federal San Mateo 19.23 N 99.12 W 2300 -/- -/- -/- -/-

8770 8770 (CIMMYT) mexicana OP Chalco Mexico Mexico Zoquiapan 19.32 N 98.85 W 2270 -/- -/- -/- -/-

11386 11386 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.27 N 98.92 W 2200 -/- -/- -/- -/-

11386 11386 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.27 N 98.92 W 2200 -/- -/- -/- -/-

11374 11374 (CIMMYT) mexicana OP Chalco Mexico Mexico Ixtapaluca 19.35 N 98.88 W 2200 -/- -/- -/- -/-

11364 11364 (CIMMYT) mexicana OP Chalco Mexico Mexico Tlalmanalco 19.20 N 98.80 W 2400 -/- -/- -/- -/-

11362 11362 (CIMMYT) mexicana OP Chalco Mexico Distrito Federal

Xochimilco 19.25 N 99.08 W 2200 -/- -/- -/- -/-

11371 11371 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan Puruandiro 20.08 N 101.52 W 2000 -/- -/- -/- -/-

11367 11367 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan Chucandiro 19.90 N 101.33 W 1800 -/- -/- -/- -/-

11370 11370 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan Puruandiro 20.08 N 101.52 W 2000 -/- -/- -/- -/-

11372 11372 (CIMMYT) mexicana OP Central

Plateau Mexico Guanajuato Moroleon 20.08 N 101.22 W 2100 -/- -/- -/- -/-

11373 11373 (CIMMYT) mexicana OP Central Plateau

Mexico Guanajuato Pinicuaro 20.05 N 101.23 W 2150 -/- -/- -/- -/-

12823 12823 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.25 N 98.85 W 2130 -/- -/- -/- -/-

12823 12823 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.25 N 98.85 W 2130 -/- -/- -/- -/-

11408 11408 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan

Villa

Escalante 19.40 N 101.60 W 2320 -/- -/- -/- -/-

12825 12825 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan Copandaro 19.88 N 101.22 W 1650 -/- -/- -/- -/-

11409 11409 (CIMMYT) mexicana OP Chalco Mexico Mexico Texcoco 19.50 N 98.92 W 2200 -/- -/- -/- -/-

11409 11409 (CIMMYT) mexicana OP Chalco Mexico Mexico Texcoco 19.50 N 98.92 W 2200 -/- -/- -/- -/-

11399 11399 (CIMMYT) mexicana OP Chalco Mexico Mexico Amecameca 19.13 N 98.77 W 2390 -/- -/- -/- -/-

11392 11392 (CIMMYT) mexicana OP Central

Plateau Mexico Durango

Puente

Gavilanes 24.02 N 104.48 W 1950 -/- -/- -/- -/-

11394 11394 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan Degollado 20.10 N 101.90 W 1700 -/- -/- -/- -/-

13792 13792 (CIMMYT) mexicana OP Chalco Mexico Mexico El Pedregal 19.27 N 99.45 W 2500 -/- -/- -/- -/-

13788 13788 (CIMMYT) mexicana OP Chalco Mexico Puebla Santa Cruz 19.08 N 97.53 W 2425 -/- -/- -/- -/-

13791 13791 (CIMMYT) mexicana OP Chalco Mexico Puebla Tlachichuca 19.17 N 97.60 W 2355 -/- -/- -/- -/-

5630 5630 (HHI) mexicana OP Central Plateau

Mexico Jalisco La Estancia 21.50 N 101.85 W 1920 -/- +/+ -/- -/-

13790 13790 (CIMMYT) mexicana OP Chalco Mexico Puebla San Salvador 19.13 N 97.63 W 2425 -/- -/- -/- -/-

13789 13789 (CIMMYT) mexicana OP Chalco Mexico Puebla San Antonio 19.07 N 97.53 W 2440 -/- -/- -/- -/-

13793 13793 (CIMMYT) mexicana OP Chalco Mexico Mexico Chapultepec 19.20 N 99.55 W 2540 -/- -/- -/- -/-

PI566673 PI566673 (NCRPIS) mexicana OP Central

Plateau Mexico Durango Durango 24.18 N 104.40 W 1950 -/- -/- -/- -/-

8750 8750 (CIMMYT) mexicana OP Chalco Mexico Mexico Ozumba 19.05 N 98.82 W 2340 -/- -/- -/- -/-

8751 8751 (CIMMYT) mexicana OP Chalco Mexico Mexico Temamatla 19.18 N 98.88 W 2400 -/- -/- -/- -/-

8770 8770 (CIMMYT) mexicana OP Chalco Mexico Mexico Zoquiapan 19.32 N 98.85 W 2270 -/- -/- -/- -/-

11362 11362 (CIMMYT) mexicana OP Chalco Mexico Distrito Federal

Xochimilco 19.25 N 99.08 W 2200 -/- -/- -/- -/-

11372 11372 (CIMMYT) mexicana OP Central Plateau

Mexico Guanajuato Moroleon 20.08 N 101.22 W 2100 -/- -/- -/- -/-

11373 11373 (CIMMYT) mexicana OP Central

Plateau Mexico Guanajuato Pinicuaro 20.05 N 101.23 W 2150 -/- -/- -/- -/-

11374 11374 (CIMMYT) mexicana OP Chalco Mexico Mexico Ixtapaluca 19.35 N 98.88 W 2200 -/- -/- -/- -/-

11387 11387 (CIMMYT) mexicana OP Nobogame Mexico Chihuahua Guadalupe y

Calvo 26.23 N 106.97 W 1850 -/- -/- -/- -/-

11392 11392 (CIMMYT) mexicana OP Central

Plateau Mexico Durango

Puente

Gavilanes 24.02 N 104.48 W 1950 -/- -/- -/- -/-

11394 11394 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Degollado 20.10 N 101.90 W 1700 -/- -/- -/- -/-

11399 11399 (CIMMYT) mexicana OP Chalco Mexico Mexico Amecameca 19.13 N 98.77 W 2390 -/- -/- -/- -/-

11400 11400 (CIMMYT) mexicana OP Chalco Mexico Mexico Los Reyes

La Paz 19.40 N 98.95 W 2200 -/- -/- -/- -/-

11409 11409 (CIMMYT) mexicana OP Chalco Mexico Mexico Texcoco 19.50 N 98.92 W 2200 -/- -/- -/- -/-

12825 12825 (CIMMYT) mexicana OP Central

Plateau Mexico Michoacan Copandaro 19.88 N 101.22 W 1650 -/- -/- -/- -/-

13788 13788 (CIMMYT) mexicana OP Chalco Mexico Puebla Santa Cruz 19.08 N 97.53 W 2425 -/- -/- -/- -/-

13789 13789 (CIMMYT) mexicana OP Chalco Mexico Puebla San Antonio 19.07 N 97.53 W 2440 -/- -/- -/- -/-

13790 13790 (CIMMYT) mexicana OP Chalco Mexico Puebla San Salvador 19.13 N 97.63 W 2425 -/- -/- -/- -/-

13791 13791 (CIMMYT) mexicana OP Chalco Mexico Puebla Tlachichuca 19.17 N 97.60 W 2355 -/- -/- -/- -/-

13792 13792 (CIMMYT) mexicana OP Chalco Mexico Mexico El Pedregal 19.27 N 99.45 W 2500 -/- -/- -/- -/-

13793 13793 (CIMMYT) mexicana OP Chalco Mexico Mexico Chapultepec 19.20 N 99.55 W 2540 -/- -/- -/- -/-

11066 11066 (LMP) mexicana OP Central

Plateau Mexico Jalisco

10 km S of

Degollado 20.37 N 102.18 W 1625 -/- -/- -/- -/-

JSG Y

LOS-3 JSG Y LOS -3 mexicana OP

Central

Plateau Mexico Jalisco Ayotlan 20.42 N 102.35 W 1520 -/- -/- -/- -/-

8779 T. A. Kato K-71-7 parviglumis OP Balsas Mexico Mexico Valle De

Bravo 19.17 N 100.32 W 1000 -/- -/- -/- -/-

JSG-187 J. Sanchez JSG-187 parviglumis OP Balsas Mexico Michoacan Huetamo 18.82 N 100.97 W 740 -/- -/- -/- -/-

JSG Y LOS-113

Sanchez & Ordaz JSG Y LOS -113

parviglumis OP Balsas Mexico Guerrero Teloloapan 18.32 N 99.80 W 1500 -/- -/- -/- -/-

JSG Y SMH-355

Sanchez & Montes JSG Y SMH -355

parviglumis OP Jalisco Mexico Jalisco Ejutla 19.95 N 104.00 W 950 -/- -/- -/- -/-

8759 T. A. Kato K-67-12 parviglumis OP Balsas Mexico Guerrero Acapetlahua

ya 18.33 N 100.12 W 970 -/- -/- -/- -/-

PI566688 Wilkes & Sanchez

WS-92-12 parviglumis OP Balsas Mexico Guerrero Zacatlan 18.38 N 99.97 W 1720 -/- -/- -/- -/-

JSG Y

LOS-172

Sanchez & Ordaz JSG

Y LOS -172 parviglumis OP Balsas Mexico Mexico

Valle De

Bravo 19.17 N 100.20 W 1620 -/- -/- -/- -/-

8782 H. G. Wilkes W-71-3 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.35 N 100.18 W 1050 -/- -/- -/- -/-

JSG-377 J. Sanchez JSG-377 parviglumis OP Balsas Mexico Guerrero Tecoanapa 17.00 N 99.28 W 625 -/- -/- -/- -/-

C-9-78 Cervantes C-9-78 parviglumis OP Balsas Mexico Guerrero Ixcateopan 18.33 N 99.80 W 1640 -/- -/- -/- -/-

11376 T. A. Kato K-69-14 parviglumis OP Balsas Mexico Guerrero El Rincon 17.28 N 99.50 W 1150 -/- -/- -/- -/-

11401 Wilkes & Sanchez

WS-92-10 parviglumis OP Balsas Mexico Guerrero Huitzuco 18.28 N 99.18 W 1225 -/- -/- -/- -/-

JSG Y

LOS-178

Sanchez & Ordaz JSG

Y LOS -178 parviglumis OP Balsas Mexico Mexico Otzoloapan 19.17 N 100.32 W 1320 -/- -/- -/- -/-

JSG-391 J. Sanchez JSG-391 parviglumis OP Balsas Mexico Mexico Zacazonapan 19.08 N 100.27 W 1420 -/- -/- -/- -/-

JSG Y MAS-400

Sanchez & Aguilar JSG Y MAS-400

parviglumis OP Jalisco Mexico Jalisco Jirosto 19.77 N 104.75 W 480 -/- -/- -/- -/-

8766 T. A. Kato K-67-23 parviglumis OP Balsas Mexico Michoacan Tuzantla 19.18 N 100.55 W 1040 -/- -/- -/- -/-

JSG Y

SMH-352

Sanchez & Montes

JSG Y SMH -352 parviglumis OP Jalisco Mexico Jalisco Ejutla 19.92 N 104.15 W 1140 -/- -/- -/- -/-

IC #3 Iltis & Cochrane Site 3 parviglumis OP Balsas Mexico Guerrero

site 2,

Teloloapan Hwy

18.33 N 99.83 W 1600 -/- -/- -/- -/-

PI566691 Wilkes & Sanchez

WS-92-? parviglumis OP Balsas Mexico Michoacan Tzitzio 19.45 N 100.90 W 1100 -/- -/- -/- -/-

JSG Y LOS-130

Sanchez & Ordaz JSG Y LOS -130

parviglumis OP Balsas Mexico Michoacan Tzitzio 19.60 N 100.92 W 1500 -/- -/- -/- -/-

JSG-379 J. Sanchez JSG-379 parviglumis OP Balsas Mexico Guerrero Mochitlan 17.28 N 99.48 W 765 -/- -/- -/- -/-

8765 T. A. Kato K-67-22 parviglumis OP Balsas Mexico Michoacan Tuzantla 19.08 N 100.47 W 950 -/- -/- -/- -/-

C-17-78 Cervantes C-17-78 parviglumis OP Balsas Mexico Michoacan Huetamo 18.83 N 100.93 W 710 -/- -/- -/- -/-

JSG Y

LOS-74

Sanchez & Ordaz JSG

Y LOS -74 parviglumis OP Jalisco Mexico Jalisco

Villa

Purificacion 19.70 N 104.82 W 520 -/- -/- -/- -/-

C-14-78 Cervantes C-14-78 parviglumis OP Balsas Mexico Mexico Luvianos 18.98 N 100.32 W 1200 -/- -/- -/- -/-

PI384064 H. G. Wilkes Wilkes 1 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.35 N 99.85 W 1550 -/- -/- -/- -/-

8760 T. A. Kato K-67-14 parviglumis OP Balsas Mexico Michoacan Tiquiche 18.85 N 100.90 W 720 -/- -/- -/- -/-

JSG-378 J. Sanchez JSG-378 parviglumis OP Balsas Mexico Guerrero Tierra

Colorada 17.18 N 99.52 W 420 -/- -/- -/- -/-

JSG Y

MAS-401

Sanchez & Aguilar

JSG Y MAS-401 parviglumis OP Jalisco Mexico Jalisco Toliman 19.53 N 104.07 W 1405 -/- -/- -/- -/-

JSG Y

LOS-126

Sanchez & Ordaz JSG

Y LOS -126 parviglumis OP Balsas Mexico Michoacan

Benito

Juarez 19.28 N 100.42 W 1100 -/- -/- -/- -/-

8763 T. A. Kato K-67-20 parviglumis OP Balsas Mexico Mexico Tingambato 19.22 N 100.28 W 1110 -/- -/- -/- -/-

8761 T. A. Kato K-67-15 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.37 N 100.87 W 1120 -/- -/- -/- -/-

BK Site 4 Beadle & Kato Site 4 parviglumis OP Balsas Mexico Guerrero 1 mile S of

Palo Blanco 17.42 N 99.50 W 1350 -/- -/- -/- -/-

11407 Wilkes & Sanchez

WS-92-16 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.60 N 100.92 W 1500 -/- -/- -/- -/-

11406 Wilkes & Sanchez

WS-92-15 parviglumis OP Balsas Mexico Michoacan

Benito

Juarez 19.22 N 100.52 W 880 -/- -/- -/- -/-

11353 T. A. Kato K-67-8 parviglumis OP Balsas Mexico Guerrero Iguala 18.35 N 99.78 W 1570 -/- -/- -/- -/-

JSG Y LOS-121

Sanchez & Ordaz JSG Y LOS -121

parviglumis OP Balsas Mexico Guerrero Acapetlahua

ya 18.38 N 100.07 W 1150 -/- -/- -/- -/-

8784 G. Beadle B-72-1 parviglumis OP Balsas Mexico Guerrero El Salado 17.40 N 99.40 W 1300 -/- -/- -/- -/-

8758 T. A. Kato K-67-7 parviglumis OP Balsas Mexico Guerrero Ixcateopan 18.37 N 99.77 W 1560 -/- -/- -/- -/-

JSG Y

LOS-109

Sanchez & Ordaz JSG

Y LOS -109 parviglumis OP Balsas Mexico Guerrero Chilpancingo 17.40 N 99.47 W 1260 -/- -/- -/- -/-

JSG-203 J. Sanchez JSG-203 parviglumis OP Jalisco Mexico Jalisco Juchitlan 19.98 N 104.05 W 1000 -/- -/- -/- -/-

967 B. Benz 967 parviglumis OP Jalisco Mexico Jalisco El Rodeo, below La

Lima

19.55 N 104.05 W 1460 -/- -/- -/- -/-

JSG-387 J. Sanchez JSG-387 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.35 N 99.83 W 1560 -/- -/- -/- -/-

JSG-193 J. Sanchez JSG-193 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.45 N 100.90 W 1100 -/- -/- -/- -/-

11357 T. A. Kato K-67-13 parviglumis OP Balsas Mexico Michoacan Huetamo 18.82 N 100.97 W 740 -/- -/- -/- -/-

8762 T. A. Kato K-67-17 parviglumis OP Balsas Mexico Mexico Luvianos 18.90 N 100.15 W 1390 -/- -/- -/- -/-

8783 H. G. Wilkes W-71-4 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.32 N 99.80 W 1580 -/- -/- -/- -/-

JSG-192 J. Sanchez JSG-192 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.37 N 100.87 W 1120 -/- -/- -/- -/-

JSG-374 J. Sanchez JSG-374 parviglumis OP Balsas Mexico Morelos Tepoztlan 18.97 N 99.03 W 1580 -/- -/- -/- -/-

JSG Y

LOS-159

Sanchez & Ordaz JSG

Y LOS -159 parviglumis OP Balsas Mexico Mexico Malinalco 18.95 N 99.50 W 1850 -/- -/- -/- -/-

11404 Wilkes & Sanchez

WS-92-13 parviglumis OP Balsas Mexico Mexico Amatepec 18.65 N 100.40 W 1180 -/- -/- -/- -/-

JSG Y MAS-264

Sanchez & Aguilar JSG Y MAS -264

parviglumis OP Jalisco Mexico Nayarit Amatlan De

Canas 20.80 N 104.40 W 900 -/- -/- -/- -/-

JSG-385 J. Sanchez JSG-385 parviglumis OP Balsas Mexico Guerrero Ixcateopan 18.50 N 99.78 W 1795 -/- -/- -/- -/-

JSG Y

MAS-402

Sanchez & Aguilar

JSG Y MAS-402 parviglumis OP Jalisco Mexico Jalisco Guachinango 20.63 N 104.42 W 1385 -/- -/- -/- -/-

JSG Y

LOS-43

Sanchez & Ordaz JSG

Y LOS -43 parviglumis OP Jalisco Mexico Jalisco Guachinango 20.80 N 104.55 W 1090 -/- -/- -/- -/-

JSG Y LOS-161

Sanchez & Ordaz JSG Y LOS -161

parviglumis OP Balsas Mexico Mexico Tejupilco 18.90 N 100.20 W 1320 -/- -/- -/- -/-

MAS-15 M. Aguilar MAS-15 parviglumis OP Jalisco Mexico Colima Minatitlan 19.45 N 104.03 W 1100 -/- -/- -/- -/-

11403 Wilkes & Sanchez

WS-92-12 parviglumis OP Balsas Mexico Guerrero Zacatlan 18.38 N 99.97 W 1720 -/- -/- -/- -/-

8776 T. A. Kato K-71-4 parviglumis OP Balsas Mexico Guerrero Palo Blanco 17.40 N 99.47 W 900 -/- -/- -/- -/-

11361 T. A. Kato K-67-25 parviglumis OP Balsas Mexico Michoacan Benito

Juarez 19.27 N 100.47 W 1235 -/- -/- -/- -/-

JSG-197 J. Sanchez JSG-197 parviglumis OP Balsas Mexico Oaxaca S. Pedro

Juchatengo 16.33 N 97.03 W 1120 -/- -/- -/- -/-

JSG Y

LOS-120

Sanchez & Ordaz JSG

Y LOS -120 parviglumis OP Balsas Mexico Guerrero

Acapetlahua

ya 18.37 N 100.03 W 1500 -/- -/- -/- -/-

JSG-191 J. Sanchez JSG-191 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.38 N 100.82 W 1000 -/- -/- -/- -/-

PI566686 Wilkes & Sanchez

WS-92-10 parviglumis OP Balsas Mexico Guerrero Huitzuco 18.28 N 99.18 W 1225 -/- -/- -/- -/-

JSG Y LOS-142

Sanchez & Ordaz JSG Y LOS -142

parviglumis OP Jalisco Mexico Jalisco Jilotlan 19.30 N 103.08 W 1460 -/- -/- -/- -/-

11355 T. A. Kato K-67-10 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.40 N 99.90 W 1800 -/- -/- -/- -/-

JSG Y

LOS-119

Sanchez & Ordaz JSG

Y LOS -119 parviglumis OP Balsas Mexico Guerrero Ixcapuzalco 18.40 N 99.97 W 1620 -/- -/- -/- -/-

8767 T. A. Kato K-67-24 parviglumis OP Balsas Mexico Michoacan Tuzantla 19.22 N 100.52 W 880 -/- -/- -/- -/-

JSG Y

LOS-176

Sanchez & Ordaz JSG

Y LOS -176 parviglumis OP Balsas Mexico Mexico Otzoloapan 19.15 N 100.38 W 1260 -/- -/- -/- -/-

JSG Y LOS-40

Sanchez & Ordaz JSG Y LOS -40

parviglumis OP Jalisco Mexico Jalisco Mascota 20.80 N 104.58 W 620 -/- -/- -/- -/-

JSG-382 J. Sanchez JSG-382 parviglumis OP Balsas Mexico Guerrero Chilpancingo 17.43 N 99.48 W 1300 -/- -/- -/- -/-

9477 M. Puga TEO:

BALSAS parviglumis OP Jalisco Mexico Jalisco

Cerro La

Mesa 19.82 N 104.18 W 850 -/- -/- -/- -/-

11388 Wilkes, Sanchez &

Taba WST-85-3 parviglumis OP Balsas Mexico Oaxaca Oaxaca 16.33 N 97.03 W 1120 -/- -/- -/- -/-

AYA 32 MR24 mays Inbred Chullpi Peru N/A N/A 12.93 S 74.25 W 2350 -/- -/- -/- -/-

CUN 465 MR26 mays Inbred Pollo Colombia N/A N/A 5.05 N 73.50 W 1600 +/- +/- -/- -/-

URG II MR28 mays Inbred Cateto Sulino

Uruguay N/A N/A 31.70 S 56.00 W N/A -/- -/- -/- -/-

PI213793 MR02 mays Inbred Assiniboine USA N/A N/A 46.82 N 100.82 W 457 -/- -/- +/- -/-

PI317675 MR12 mays Inbred Havasupai USA N/A N/A 36.25 N 112.67 W 1067 -/- -/- -/- -/-

PI217408 MR14 mays Inbred Longfellow

Flint Canada N/A N/A 42.25 N 71.50 W N/A -/- -/- +/+ -/-

PI218130 MR19 mays Inbred Santa

Domingo USA N/A N/A 35.52 N 106.35 W 1585 -/- -/- -/- -/-

PI269743 MR20 mays Inbred Shoe Peg USA N/A N/A 36.78 N 92.22 W 213 -/- -/- +/+ -/-

BOV 635 MR05 mays Inbred Cateto Bolivia N/A N/A 16.60 S 67.25 W 1801 -/- +/+ -/- -/-

CUB 65 MR11 mays Inbred Cuban Flint Cuba N/A N/A 23.08 N 82.67 W 183 +/+ -/- -/- -/-

MOR 17 MR16 mays Inbred Pepetilla Mexico N/A N/A 18.35 N 99.53 W 747 -/- -/- -/- -/-

NAY 15 MR18 mays Inbred Reventador Mexico N/A N/A 22.33 N 105.38 W 46 -/- -/- -/- -/-

VEN 568 MR01 mays Inbred Araguito Venezuela N/A N/A 8.90 N 64.22 W 183 -/- -/- -/- -/-

VEN 604 MR04 mays Inbred Canilla Venezuela N/A N/A 8.90 N 64.22 W 183 -/- -/- -/- -/-

TAM 125 MR22 mays Inbred Tuxpeno Mexico N/A N/A 22.28 N 97.88 W 150 -/- -/- -/- -/-

BOV 344 MR17 mays Inbred Pisankalla Bolivia N/A N/A 21.52 S 64.75 W 1738 -/- -/- -/- -/-

CHI 349 MR10 mays Inbred Cristalino

Norteno Chile N/A N/A 39.92 S 72.50 W 7 -/- -/- -/- -/-

JAL 142 MR15 mays Inbred Palomero De

Jalisco Mexico N/A N/A 20.03 N 103.68 W 2520 -/- -/- -/- -/-

OAX 68 MR03 mays Inbred Bolita Mexico N/A N/A 17.47 N 97.30 W 1646 +/- -/- -/- -/-

OAX 70 MR23 mays Inbred Zapalote

Chico Mexico N/A N/A 16.33 N 95.23 W 46 -/- -/- -/- -/-

CHS 86 MR07 mays Inbred Comiteco Mexico N/A N/A 16.25 N 92.13 W 1555 +/+ -/- -/- -/-

RGS VII MR09 mays Inbred Cravo

Riogranense Brazil N/A N/A 30.00 S 53.00 W 366 -/- +/+ -/- -/-

SIN 2 MR06 mays Inbred Chapalote Mexico N/A N/A 24.80 N 107.42 W 61 -/- -/- -/- -/-

VEN 453 MR08 mays Inbred Costeno Venezuela N/A N/A 9.02 N 63.72 W 146 -/- +/+ -/- -/-

JAL 43 MR21 mays Inbred Tabloncillo Mexico N/A N/A 19.98 N 104.27 W 1330 -/- -/- -/- -/-

PI311237 MR13 mays Inbred Hickory

King USA N/A N/A 37.22 N 80.42 W N/A -/- -/- +/+ -/-

GTO 73 GTO 73 (INIFAP) mays OP Conico

Norteno Mexico N/A N/A 20.37 N 101.07 W 1751 -/- -/- -/- -/-

VEN 453 VEN 453 (ICA) mays OP Costeno Venezuela N/A N/A 9.02 N 63.72 W 146 -/- +/- -/- -/-

JAL 43 JAL 43 (CIMMYT) mays OP Tabloncillo Mexico N/A N/A 19.98 N 104.27 W 1330 -/- -/- -/- -/-

SIN 2 SIN 2 (INIFAP) mays OP Chapalote Mexico N/A N/A 24.80 N 107.42 W 61 -/- -/- -/- -/-

NAY 15 NAY 15 (INIFAP) mays OP Reventador Mexico N/A N/A 22.33 N 105.38 W 46 -/- -/- -/- -/-

PI217408 PI217408 (NCRPIS) mays OP Lonfellow

Flint USA N/A N/A 42.25 N 71.50 W N/A -/- -/- +/- -/-

OAX 70 OAX 70 (CIMMYT) mays OP Zapalote

Chico Mexico N/A N/A 16.33 N 95.23 W 46 -/- -/- -/- -/-

PI213793 PI213793 (NCRPIS) mays OP Assiniboine USA N/A N/A 46.82 N 100.82 W 457 -/- -/- -/- -/-

PI269743 PI269743 (NCRPIS) mays OP Shoe Peg USA N/A N/A 36.78 N 92.22 W 213 +/+ -/- -/- -/-

VEN 630 VEN 630 (NRC) mays OP Cacao Venezuela N/A N/A 8.60 N 71.17 W 201 -/- +/- -/- -/-

VEN 568 VEN 568 (NRC) mays OP Araguito Venezuela N/A N/A 8.90 N 64.22 W 183 -/- +/+ -/- -/-

OAX 68 OAX 68 (INIFAP) mays OP Bolita Mexico N/A N/A 17.47 N 97.30 W 1646 -/- -/- -/- -/-

RGS VII RGS VII (CIMMYT) mays OP

Cravo

Riograndense

Brazil N/A N/A 30.00 S 53.00 W 366 -/- +/+ -/- -/-

BOV 635 BOV 635 (NRC) mays OP Cateto Bolivia N/A N/A 16.60 S 67.25 W 1801 -/- -/- -/- -/-

BOV 344 BOV 344 (ICA) mays OP Pisankalla Bolivia N/A N/A 21.52 S 64.75 W 1738 -/- -/- -/- -/-

BOV 587 BOV 587 (NRC) mays OP Pororo Bolivia N/A N/A 16.37 S 60.95 W 330 -/- -/- -/- -/-

SUR 797 SUR 797 (CIMMYT) mays OP Cateto

Nortista Suriname N/A N/A 5.75 N 55.22 W 61 +/- -/- -/- -/-

CUN 465 CUN 465 (NRC) mays OP Pollo Colombia N/A N/A 5.05 N 73.50 W 1600 -/- +/+ -/- -/-

AYA 32 AYA 32 (PCIM) mays OP Chullpi Peru N/A N/A 12.93 S 74.25 W 2350 -/- +/+ -/- -/-

ARG 461 ARG 461 (NRC) mays OP Capia

Amarillo Argentina N/A N/A 23.20 S 65.35 W 2287 -/- +/+ -/- -/-

URG II URG II (CIMMYT) mays OP Cateto Sulino

Uruguay N/A N/A 31.70 S 56.00 W N/A -/- -/- -/- -/-

CHI 349 CHI 349 (NCGRP) mays OP Cristalino

Norteno Chile N/A N/A 39.92 S 72.50 W 7 -/- -/- +/- -/-

MEX 6 MEX 6 (INIFAP) mays OP Palomero

Toluqueno Mexico N/A N/A 19.28 N 99.67 W 2652 -/- -/- -/- -/-

CHH 128 CHH 128 (INIFAP) mays OP Cristalino De

Chihuahua Mexico N/A N/A 29.22 N 108.13 W 2095 -/- -/- -/- -/-

CHH 158 CHH 158 (INIFAP) mays OP Azul Mexico N/A N/A 28.55 N 107.48 W 2040 -/- -/- -/- -/-

CHH 166 CHH 166 (INIFAP) mays OP Apachito Mexico N/A N/A 27.98 N 107.58 W 2400 -/- -/- -/- -/-

CHS 39 CHS 39 (INIFAP) mays OP Comiteco Mexico N/A N/A 15.23 N 92.27 W 1400 +/- -/- -/- -/-

GTO 69 GTO 69 (INIFAP) mays OP Celaya Mexico N/A N/A 20.48 N 100.97 W 1812 -/- -/- -/- -/-

JAL 753 JAL 753 (INIFAP) mays OP Serrano De

Jalisco Mexico N/A N/A 20.03 N 103.68 W 2520 -/- -/- -/- -/-

CHS 81 CHS 81 (INIFAP) mays OP Olotillo Mexico N/A N/A 16.60 N 92.72 W 686 +/- -/- -/- -/-

CHS 695 CHS 695 (INIFAP) mays OP Oloton Mexico N/A N/A 16.80 N 92.55 W 2390 +/- +/- -/- -/-

GRO 383 GRO 383 (INIFAP) mays OP Ancho Mexico N/A N/A 18.42 N 99.55 W 1200 -/- -/- -/- -/-

MEX 108 MEX 108 (INIFAP) mays OP Conico Mexico N/A N/A 19.10 N 99.58 W 2520 -/- -/- -/- -/-

MEX 212 MEX 212 (INIFAP) mays OP Cacahuacintl

e Mexico N/A N/A 19.30 N 99.65 W 2620 -/- -/- -/- -/-

PUE 510 PUE 510 (INIFAP) mays OP Elotes

Conicos Mexico N/A N/A 18.97 N 98.30 W 2200 -/- -/- -/- -/-

ZAC 180 ZAC 180 (INIFAP) mays OP Elotes

Occidentales Mexico N/A N/A 22.65 N 103.70 W 2050 -/- -/- -/- -/-

CHS 30 CHS 30 (CIMMYT) mays OP Vandeno Mexico N/A N/A 16.50 N 92.50 W 614 -/- -/- -/- -/-

GTO 191 GTO 191 (INIFAP) mays OP Zamorano

Amarillo Mexico N/A N/A 21.12 N 101.68 W 1870 -/- +/+ -/- -/-

VER 457 VER 457 (INIFAP) mays OP Coscomatepe

c Mexico N/A N/A 19.20 N 97.03 W 1800 -/- -/- -/- -/-

ZAC 182 ZAC 182 (INIFAP) mays OP Dulce De

Jalisco Mexico N/A N/A 22.65 N 103.70 W 2050 -/- -/- -/- -/-

MIC 328 MIC 328 (INIFAP) mays OP Mushito Mexico N/A N/A 19.68 N 102.12 W 2200 -/- -/- -/- -/-

SIN 34 SIN 34 (INIFAP) mays OP Dulcillo Del

Noroeste Mexico N/A N/A 25.07 N 107.50 W 500 -/- -/- -/- -/-

OAX 570 OAX 570 (INIFAP) mays OP Mixteco Mexico N/A N/A 17.42 N 97.48 W 2600 -/- -/- -/- -/-

SON 117 SON 117 (INIFAP) mays OP Blandito De

Sonora Mexico N/A N/A 28.97 N 109.40 W 410 -/- -/- -/- -/-

CHS 26 CHS 26 (INIFAP) mays OP Tepecintle Mexico N/A N/A 15.43 N 92.90 W 107 -/- -/- -/- -/-

NAY 191 NAY 191 (CIMMYT) mays OP Bofo Mexico N/A N/A 21.38 N 104.13 W 1000 -/- -/- -/- -/-

CHS 29 CHS 29 (CIMMYT) mays OP Tehua Mexico N/A N/A 15.75 N 92.72 W 1000 -/- +/- -/- -/-

CHS 521 CHS 521 (INIFAP) mays OP Zapalote

Grande Mexico N/A N/A 16.72 N 93.72 W 730 -/- -/- -/- -/-

CHS 650 CHS 650 (INIFAP) mays OP Motozinteco Mexico N/A N/A 15.37 N 92.25 W 1270 +/- -/- -/- -/-

CHH 150 CHH 150 (INIFAP) mays OP Palomero

Tipo

Chihuahua

Mexico N/A N/A 29.35 N 107.75 W 2140 -/- -/- -/- -/-

CAM 48 CAM 48 (INIFAP) mays OP Nal-Tel Mexico N/A N/A 19.78 N 90.12 W 50 +/+ -/- -/- -/-

CHH 121 CHH 121 (INIFAP) mays OP Tuxpeno

Norteno Mexico N/A N/A 30.90 N 108.17 W 1470 -/- +/- -/- -/-

CHS 196 CHS 196 (INIFAP) mays OP Nal-Tel De

Altura Mexico N/A N/A 15.47 N 92.15 W 2300 +/- -/- -/- -/-

COA 21 COA 21 (INIFAP) mays OP Tuxpeno

Norteno Mexico N/A N/A 25.43 N 102.18 W 1400 +/- -/- -/- -/-

COA 25 COA 25 (INIFAP) mays OP Raton Mexico N/A N/A 25.75 N 102.97 W 1610 +/- -/- -/- -/-

GRO 157 GRO 157 (INIFAP) mays OP Conejo Mexico N/A N/A 17.12 N 100.47 W 30 -/- +/- -/- -/-

NAY 16 NAY 16 (CIMMYT) mays OP Tabloncillo

Perla Mexico N/A N/A 22.08 N 105.25 W 46 -/- -/- -/- -/-

NAY 24 NAY 24 (INIFAP) mays OP Harinoso De

Ocho Mexico N/A N/A 21.95 N 105.30 W 46 -/- -/- -/- -/-

NAY 185 NAY 185 (CIMMYT) mays OP Tablilla De

Ocho Mexico N/A N/A 21.68 N 104.07 W 1100 -/- -/- -/- -/-

OAX 565 OAX 565 (INIFAP) mays OP Serrano

Mixe Mexico N/A N/A 17.13 N 96.02 W 2350 -/- +/+ -/- -/-

OAX 566 OAX 566 (INIFAP) mays OP Mixeno Mexico N/A N/A 17.22 N 96.05 W 1850 -/- -/- -/- -/-

QOO 20 QOO 20 (INIFAP) mays OP Dzit Bacal Mexico N/A N/A 21.25 N 86.75 W 10 +/+ -/- -/- -/-

SIN 17 SIN 17 (INIFAP) mays OP Elotero De

Sinaloa Mexico N/A N/A 23.50 N 106.30 W 200 -/- -/- -/- -/-

SON 105 SON 105 (INIFAP) mays OP Onaveno Mexico N/A N/A 28.32 N 108.97 W 820 -/- -/- -/- -/-

VER 311 VER 311 (INIFAP) mays OP Arrocillo

Amarillo Mexico N/A N/A 19.80 N 97.25 W 2220 -/- +/+ -/- -/-

JAL 78 JAL 78 (INIFAP) mays OP Dulce De

Jalisco Mexico N/A N/A 21.18 N 102.88 W 1880 -/- -/- -/- -/-

B2 B2 (Goodman) mays OP Reid Yellow

Dent USA N/A N/A N/A N/A N/A -/- -/- +/+ -/-

VEN 604 VEN 604 (ICA) mays OP Canilla Venezuela N/A N/A 8.90 N 64.22 W 183 -/- -/- -/- N/A

COR 334 COR 334 (NRC) mays OP Cariaco Colombia N/A N/A 8.18 N 76.07 W 200 -/- +/- -/- -/-

ECU 458 ECU 458 (NRC) mays OP Chillo Ecuador N/A N/A 2.85 S 78.67 W 2195 -/- +/+ -/- -/-

ECU 964 ECU 964 (ICA) mays OP Chococeno Ecuador N/A N/A 0.67 S 79.50 W 46 -/- +/+ -/- -/-

PUN 4 PUN 4 (PCIM) mays OP Confite

Puneno Peru N/A N/A 15.93 S 69.88 W 3900 -/- +/+ -/- -/-

MAG 443 MAG 443 (NCGRP) mays OP Guirua Colombia N/A N/A 10.48 N 73.25 W 1860 +/- +/- -/- -/-

CUN 372 CUN 372 (ICA) mays OP Imbricado Colombia N/A N/A 4.95 N 74.38 W 2625 -/- +/+ -/- -/-

CUZ 66 CUZ 66 (PCIM) mays OP Kculli Peru N/A N/A 13.53 S 71.90 W 3268 -/- +/- -/- -/-

NAR 426 NAR 426 (ICA) mays OP Montana Colombia N/A N/A 1.18 N 77.25 W 2500 +/- +/- -/- -/-

BOY 462 BOY 462 (NRC) mays OP Pira Colombia N/A N/A 5.02 N 73.45 W 900 +/+ -/- -/- -/-

VEN 485 VEN 485 (ICA) mays OP Pira Venezuela N/A N/A 8.00 N 72.00 W 412 -/- -/- -/- -/-

APC 13 APC 13 (PCIM) mays OP Pisccorunto Peru N/A N/A 13.67 S 73.37 W 2900 -/- +/+ -/- -/-

SAN 329 SAN 329 (NRC) mays OP Sabanero Colombia N/A N/A 7.32 N 72.47 W 2626 +/+ -/- -/- N/A

YUC 148 YUC 148 (INIFAP) mays OP Nal-Tel Mexico N/A N/A 20.3 N 89.43 W 30 +/+ -/- -/- N/A

MOR 99 MOR 99 (INIFAP) mays Pepitilla Mexico N/A N/A 18.68 N 99.12 W 1040 -/- -/- -/- -/-

JAL 44 JAL 44 (CIMMYT) mays OP Jala Mexico N/A N/A 19.95 N 104.27 W 1300 -/- -/- -/- -/-

BOV 331 BOV 331 (ICA) mays OP Aysuma Bolivia N/A N/A 19.03 S 65.27 W 2127 -/- +/- -/- -/-

ARG 499 ARG 499 (CIMMYT) mays OP Capia Blanco Argentina N/A N/A 23.20 S 65.35 W 2287 -/- +/+ -/- -/-

SUR I SUR I (CIMMYT) mays OP Cateto Nortista

Precoce Suriname N/A N/A 5.75 N 55.22 W 61 -/- -/- -/- -/-

URG II URG II (CIMMYT) mays OP Cateto

Sulino Uruguay N/A N/A 31.70 S 56.00 W N/A -/- -/- -/- -/-

MEX 48 MEX 48 (INIFAP) mays OP Chalqueno Mexico N/A N/A 19.68 N 99.13 W 2256 -/- -/- -/- -/-

PUE 109 PUE 109 (INIFAP) mays OP Conico Mexico N/A N/A 18.23 N 97.57 W 2104 -/- -/- -/- -/-

BOV 992 BOV 992 (ICA) mays OP Coroico Bolivia N/A N/A 13.08 S 64.82 W 200 -/- +/+ -/- -/-

CUB 63 CUB 63 (CIMMYT) mays OP Cuban Flint Cuba N/A N/A 20.67 N 75.75 W 91 -/- -/- -/- -/-

SP III SP III (CIMMYT) mays OP Dente

Paulista Brazil N/A N/A 22.00 S 48.00 W 518 -/- +/+ -/- -/-

GUA 130 GUA 130 (CIMMYT) mays OP Dzit Bacal Guatemala N/A N/A 14.43 N 89.67 W 610 +/- +/- -/- -/-

CHI 421 CHI 421 (NRC) mays OP Harinoso

Tarapaqueno Chile N/A N/A 19.92 S 69.52 W N/A -/- +/- -/- -/-

BOV 961 BOV 961 (NRC) mays OP Karapampa Bolivia N/A N/A 21.83 S 64.13 W 1976 -/- +/- -/- -/-

BOV 567 BOV 567 (ICA) mays OP Morado Bolivia N/A N/A 16.47 S 67.47 W 2270 -/- +/+ -/- -/-

GUA 14 GUA 14 (INIFAP) mays OP Serrano Guatemala N/A N/A 15.28 N 91.43 W 1738 -/- +/- -/- -/-

TRN 10 TRN 10 (INIFAP) mays OP Tuson Trinidad N/A N/A 10.58 N 61.12 W 20 -/- -/- -/- -/-

PI214195 PI214195 (NCRPIS) mays OP Longfellow Canada N/A N/A 43.58 N 80.23 W 488 -/- -/- +/- -/-

JAL 127 JAL 127 (INIFAP) mays OP Mountain

Yellow Mexico N/A N/A 19.95 N 103.77 W 2060 -/- -/- -/- -/-

JAL 142 JAL 142 (INIFAP) mays OP Palomero De

Jalisco Mexico N/A N/A 20.03 N 103.68 W 2520 -/- -/- -/- -/-

Table S5. Primers used for ZmYAB2.1 genotyping and expression analyses

Primer Sequence (5’ – 3’) Description

CJ199 AGAGAAGGCGTTCGAGCATA

Marker for Spm insertion in W22 CJ198 CCACCATCAGTCACACACTT

CJ102 TGCATTTGCTTACGGTACTT

CJ096 TTCGAATGACTTGTAGTTCAG

Marker for Spm insertion in B73 CJ167 CCGAAAATGGTGTACAGTGC

CJ132 AGTGCCTTTGTTGACACTCG

CJ055 ACGTGCAGCATATGATCCAG Marker for 2bp/82bp insertions in Ki3 and Mo17

CJ085 TCAAGGTCCAAAATTTCAGC

CJ075 CTTATGTCCGGGTTGTTTGC qPCR primer pair for ZmYAB2.1

CJ085 TCAAGGTCCAAAATTTCAGC

wh182 CCAAGGCCAACAGAGAGAAA qPCR primer pair for GRMZM2G126010 (actin gene)

wh183 CCAAACGGAGAATAGCATGAG

CJ080 CTGGATGGCCCTCTGATTAG Primer pair for ZmYAB2.1 5’ UTR allele specific expression

CJ124 GACGACATTCCTTCGCCTTG

T385 TCATGGACAACGATGAGTGG qPCR primer pair for tb1

T388 CAATAACGCACACCAGGTCC

Table S6. PCR fragment sizes for alleles used in allele specific expression analysis

Allele PCR fragment size (bp)

A619 164

Oh43 164

MR12 150

MR19 153

TIL01 164

TIL11 160

Table S7. Allele specific expression results of ZmYAB2.1 with five or more reads from Lemmon et al.

(2014)

F1 Hybrid Percentage of Reads Belonging to Maize (%) Total Number of Reads

B73 x TIL14 0 65

W22 x TIL14 0 15

Ki3 x TIL11 28 39

Ki3 x TIL03 10 31

References

Cook, J. P., M. D. McMullen, J. B. Holland, F. Tian, P. Bradbury et al., 2012 Genetic

architecture of maiuze kernel composition in the nested association mapping and inbred

association panels. Plant Physiol. 158: 824–834.

Darling, A. C. E., B. Mau, F. R. Blattner, and N. T. Perna, 2004 Mauve: multiple alignment of

conserved genomic sequence with rearrangements. Genome Res. 14: 1394–1403.

Lemmon, Z. H., R. Bukowski, Q. Sun, and J. F. Doebley, 2014 The role of cis regulatory

evolution in maize domestication. PLoS Genet. 10: e1004745.

Suppor

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in Table

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RC-NILs used in phenotyping.

Figure S2. Geographical distributions of 3 null ZmYAB2.1 alleles: W22_Spm [A], B73_Spm [B],

Mo17_82bp [C] in maize landrace populations. Ki3_2bp is not shown here as it is only present in

2 maize inbreds and 1 teosinte inbred.

Figure S3. Cartoon showing recombination breakpoint of a RC-NIL (IN0949) in between Exon 1

and Exon 2 of ZmYAB2.1. IN0949 carries maize regulatory region and mostly teosinte coding

region, while IN1207 carries full teosinte regulatory and coding regions. IN0949 displays maize

class ear internode length while IN1207 displays teosinte class ear internode length (Figure 1).

IN0949 also has lower ZmYAB2.1 expression in comparison to IN1207 (Figure 2B).

Figure S

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NI-RILs hav

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ve maize

Figure S5. ZmYAB2.1 3’UTR probe hybridized to maize ears containing the maize allele (A) or

the teosinte allele (B). Weak expression in the region homologous to the dehiscence zone

(arrows) is only seen in in the teosinte allele (B). Scale bars are 200µm.

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10 20 30 40 50 60 70 80 | | | | | | | | B73 AGCTGAGGAAGGCAATTGTTTCGAATCTGGCTGCTGCGTAGCCAGACATAGAGAATCCTTTTAGACGCAACGAGGCCCCA W22 AGCTGAGGAAGACAATTGTTTCGAATCTGGCTGCTGCGTAGCCAGACATAGAGAATCCTTTTAGACGCAACGAGGCCCCA Teo ACCTGAGGAAGGCAATTGTTTCGAATCTGGCTGCTGCGTAGCCAGACATAGAGAATCCTTTTAGACGCAACGAGGCCCCA 90 100 110 120 130 140 150 160 | | | | | | | | B73 GCAATTCATTGTGGCGTTTTGCACCCACATCAACCCGCCGCGCCCCCCTTCACAATAGGAGCCAGAACAAAAAAAAAA-- W22 GCAATTCATTGTGGCGTTTTGCACCCACATCAACCCGCCGCGCCCCCCTTCACAATAGGAGCCAGAACAAAAAAAAAA-- Teo GCAATTCATTGTGGCGTTTTGCACCCACATCAACCCGCC-------CCTTCACAATAGGAGCCAGAACAAAAAAAAAAAC 170 180 190 200 210 220 230 240 | | | | | | | | B73 ---CCACTACTCCTCTCTCTCCCTCACACACA--GAGTGAGAGAGAGAGAGACGCACAAGAAATCAGTAGGTCAATCTCT W22 ---CCACTACTCCTCTCTCTCCCTCACACACA--GAGTGAGAGAGAGAGAGACGCACAAGAAATCAGTAGGTCAATCTCT Teo CCCCCACTACTC-----TCTCTCTCACACACACATAGAGAGAGAGAGAGAGACGCACAAGAAATCAGTAGGTCAATCTCT 250 260 270 280 290 300 310 320 | | | | | | | | B73 CCTCTCACCTTTACATCGTCTTTGTATTTACAAAGTTGTTGATGACTTGATGTAGAGGCTGTACCTGCTGGTTTGCTACC W22 CCTCTCACCTTTACATCGTCTTTGTATTTACAAAGTTGTTGATGACTTGATGTAGAGGCTGTACCTGCTGGTTTGCTACC Teo CCTCTCACCTTTACATCGTCTCTGTATTTGCAAAGTTGTTGATGAGTTGATGTAGAGGCTGTACCTGCTGGTTTGCTACC 330 340 350 360 370 380 390 400 | | | | | | | | B73 CCTAGCTAGCTATATGTCGTCCAATCCTAGTTGAGCTCTCGATCTGGATGGCCCTCTGATTAGTCGTCCTTTGCTTGGTG W22 CCTAGCTAGCTATATGTCGTCCAATCCTAGTTGAGCTCTCGATCTGGATGGCCCTCTGATTAGTCGTCCTTTGCTTGGTG Teo CCTAGCTAGCTATATGTCGTCCAATCCTAGTTGAGCTCTCGATCTGGATGGCCCTCTGATTAGTCCTCCTTTGCTTGGTG 410 420 430 440 450 460 470 480 | | | | | | | | B73 GTGCCTGCTGTGTATATCGCCACCCCGGCCGCTTGCTTCCGCTAGTAGCTAGTCCCTT--CTTCTCTTCTCGATCAGTTT W22 GTGCCTGCTGTGTATATCGCCACCCCGGCCGCTTGCTTCCGCTAGTAGCTAGTCCCTT--CTTCTCTTCTCGATCAGTTT Teo GTGCCTGCTGTGTATATCGCCACCCCGGCCGCTTGCTTCCGCTAGT-------CCCTTTTCTTCTCTTCTCGATCA---- 490 500 510 520 530 540 550 560 | | | | | | | | B73 GTTTAATTGGCAGAGCGTATGTATCACACCCAAGGCGAAGGAATGTCGTCGGCGGCCCAGATCGCGCCGGTGCCGGAGCA W22 GTTTAATTGGCAGAGCGTATGTATCACACCCAAGGCGAAGGAATGTCGTCGGCGGCCCAGATCGCGCCGGTGCCGGAGCA Teo -----ATTGGCAGAGCGTATGTATCACACCCAAGGCGAAGGAATGTCGTCGGCGGCCCAGATCGCGCCGGTGCCGGAGCA 570 580 590 600 610 620 630 640 | | | | | | | | B73 TGTATGCTACGTGCACTGCAACTTCTGCAACACAATTCTCGCGGTAATATATATATAACCCCGATCTGATCTCTAGCTAG W22 TGTATGCTACGTGCACTGCAACTTCTGCAACACAATTCTCGCGGTAATATATATATAACCCCGATCTGATCTCTAGCTAG Teo TGTGTGCTACGTGCACTGCAACTTCTGCAACACAATTCTCGCGGTAATA--------ACCCCGATCTGATCTCTAGCTAG 650 660 670 680 690 700 710 720 | | | | | | | | B73 CTAGCCTCTCGATCTGTTTGCCCCCCTCAGCTCGTTTTCTCCCCCGGCTCTCTCGATCGGTTTTTTCCCTGGTTGCCGGC W22 CTAGCCTCTCGATCTGTTTGCCCCCCTCAGCTCGTTTTCTCCCCCGGCTCTCTCGATCGGTTTTTTCCCTGGTTGCCGGC Teo C----CTCTCGATCTGTTTGCCCCCCTCAGCTCGTTTTCTCCCCCGGCCCTCTCGATCGGTTT---CCCTGGTTGCCTGC 730 740 750 760 770 780 790 800 | | | | | | | | B73 CTCTGAACTGAAGCGCAGCTATATATATACAGCAGAAAGATGCATGAAAAAT----AGTTGATTGGCTGCGTGCGCCTGC W22 CTCTGAACTGAAGCGCAGCTATATATATACAGCAGAAAGATGCATGAAAAAT----AGTTGATTGGTTGCGTGCGCCTGC Teo CTCTGAACTGAAGCGCAGCTATATATATACAGCAGAAAGATGCATGAAAAATGGTTAGTTGATTGGCTGCGTGCGCCTGC 810 820 830 840 850 860 870 880 | | | | | | | | B73 ATCGTCGTCGTATAGTAGTTAGTTTTAATTTGTACGAATTGGCTGTTCCGCTTTCTGGATTTCATCAGCTTCTGGATCAT W22 ATCGTCGTCGTATAGTAGTTAGTTTTAATTTGTACGAATTGGCTGTTCCGCTTTCTGGATTTCATCAGCTTCTGGATCAT Teo ATCGTCGT---AT---AGTTAGTTTTAATTTGTGCGATTTGGCTGTTCCGCTTTCTGGATTTCATCAGCTTCTGGATCAT 890 900 910 920 930 940 950 960 | | | | | | | | B73 TTCATCAGAGCTTTTGGTCTAAATTGAAAATTGGGGATGGTTCTTGCATCAAATTAAGTCTTGGGAACAAGATGACTAAT W22 TTCATCAGAGCTTTTGGTCTAAATTGAAAATTGGGGATGGTTCTTGCATCAAATTAAGTCTTGGGAACAAGATGACTAAT Teo TTCATCAGAGCTTTTGGTCTAAATTGAAAATTGGGGATGGTTCTTGCATCAAATTAAGTCTTGGGAACAACATGACTAAT

970 980 990 1000 1010 1020 1030 1040 | | | | | | | | B73 TAGCTAGCAGTCTTAGACGAA----GCTAGTCAGCAAGTTCTCAAAGCATTCTCGCCTTCTGCAGG--CCCTCCTGTACT W22 TAGCTAGCAGTCTTAGACGAA----GCTAGTCAGCAAGTTCTCAAAGCATTCTCGCCTTCTGCAGG--CCCTCCTGTACT Teo TAGCTAGCAGTCTTAGACGAATGAAGCTAGTCAGCAAGTTCTCAAAGCATTCTCGCCTTCTGCATGCCCCCTCCTGTACT 1050 1060 1070 1080 1090 1100 1110 1120 | | | | | | | | B73 TTTTTT-CTGGGAAATTATCGTCATCATCGTTGATGAG--TGGTGGTCTTTATATTAATTAGATCTCAAGTATATATAGC W22 TTTTTT-CTGGGAAATTATCGTCATCATCGTTGATGAG--TGGTGGTCTTTATATTAATTAGATCTCAAGTATATATAGC Teo TTTTTTTCTGGGAAATTATCGTCATCATCGTTGATGAGAGTGGTGGTCTATATATTAATTAGATCTCAAGTATATATAGC 1130 1140 1150 1160 1170 1180 1190 1200 | | | | | | | | B73 TTTGTTTGCCTGCAGGTGTATATGTGTGATATTTGTACATATATAAAGCTTCTTCAGCACCGCGCTAGCTAGCTTCTTTC W22 TTTGTTTGCCTGCAGGTGTATATGTGTGATATTTGTACATATATAAAGCTTCTTCAGCACCGCGCTAGCTAGCTTCTTTC Teo TTTGTTTGCCTGCAGGTGTAT--GTGTGATGTGTACATATATATAAAGCTTCTTCAGCACCGCGCTAGCTAGCTTCTTTC 1210 1220 1230 1240 1250 1260 1270 1280 | | | | | | | | B73 GCATAACCAGAGTCCGATCCGGCCGGTGGTTAACTTATTTCAGCTAATATTTGAAACACAATACATGTATACGACATA-- W22 GCATAACCAGAGTCCGATCCGGCCGGTGGTTAACTTATTTCAGCTAATATTTGAAACACAATACATGTATACGACATA-- Teo GCATAACCAGAGTCCGATCCGGCCGGTGGTTAACTTATTTCAGCTAATATTTGAAACACAATACTTGTATACGACATATA 1290 1300 1310 1320 1330 1340 1350 1360 | | | | | | | | B73 --GGGCAATAGATTTACTCGTTACCTTCTTGATCGAACTGCAACAGAGAGTGCATGCAATAATAACCATTTTTAAGACGA W22 --GGGCAATAGATTTACTCGTTACCTTCTTGATCGAACTGCAACAGAGAGTGCATGCAATAATAACCATTTTTAAGACGA Teo TAGGGCAATAGATTTACTCGTTACCTTCTTGATCGAACTGCAACAGAGAGTGCATGCAATAATAACCATTTTTAAGACGA 1370 1380 1390 1400 1410 1420 1430 1440 | | | | | | | | B73 TAAAAAAATTTAGTGTGTACCGCGTGGATCTTTAGCTTTTATGCTTTTCTATATAATATGTAGACCATATCTCACATAAA W22 TAAAAAAATTTAGTGTGTACCGCGTGGATCTTTAGCTTTTATGCTTTTCTATATAATATGTAGACCATATCTCACATAAA Teo TAAAAAAATT-AGTGTGTACCGCGTGGATCTTTAGCTTTTATGCTTTTCTATATAATATGTAGACCATATCTCACATAAA 1450 1460 1470 1480 1490 1500 1510 1520 | | | | | | | | B73 TCGAGGTCGTGTCCTAATAAATATACTAAGAGATATATTGACTAAAACAAAGGCCTTTTT--TATTGACTAATAATAATA W22 TCGAGGTCGTGTCCTAATAAATATACTAAGAGATATATTGACTAAAACAAAGGCCTTTTT-TTATTGACTAATAATAATA Teo TCGAGGTCGTGTCCTAATAAATATACTAAGAGATATATTGACTAAAACAAAGGCCTTTTTGTTATTGACTAATAATAATA 1530 1540 1550 1560 1570 1580 1590 1600 | | | | | | | | B73 ACATATATACTCCCTCCGTTTCTTTTTAGTTGTCGATGAATAGTTTAATTTTGCACTATCCAGCGACAAGTAAAACGAAA W22 ACATATATACTCCCTCCGTTTCTTTTTAGTTGTCGCTGAATAGTTTAATTTTGCACTATCCAGCGACAACTAAAACGAAA Teo ACATATATA----------------------------------------------------------------------- 1610 1620 1630 1640 1650 1660 1670 1680 | | | | | | | | B73 CAGAGGGAGTATTAATTATTGCCCAATATCTGTACTACGTCGTAGCATTGCTACTTTTTATTCATATATT-GGAACAAAA W22 CCGAGGGAGTATTAATTATTGCCCAATATCTGTACTACGTCGTAGCATTGCTACTTTTTATTCATATATT-GGAACAAAA Teo ------------------TTGCCCAATATCTGTAC---GTCGTAGCATTGCTACTTTTTATTCATATATTTGGAACAAAA 1690 1700 1710 1720 1730 1740 1750 1760 | | | | | | | | B73 AAAA-TTCCGTTACGTAAGGTTTTTATTCATTGGAACCAAAAAAATATTGTTACGTAAGTTTGAGTACGGTTTGCTGGAA W22 AAA--TTCCGTTACGTAAGGTTTTTATTCATTGGAACCAAAAAAATATTGTTACGTAAGTTTGAGTACGGTTTGCTGGAA Teo AAAAA--CCGTTACGTAAGGTTCTTATTCATTGGAACCAAAAAA-T-TTGTTACGTAAGTTTGAATACGGTTTGCTGGAA 1770 1780 1790 1800 1810 1820 1830 1840 | | | | | | | | B73 AGAGTTTTAAAATGATAAAAAATGTTGATGTGGAATAATTAATAAACGAAGAGCGAACATATCATTACAAAATATGTATA W22 AGAGTTTTAAAATGATAAAAAATGTTGATGTGGAATAATTAATAAACGAAGAGCGAACATATCATTACAAAATATGTATA Teo AGAGTTTTAAAATGATCAAAAATGTTGATGTGGAATAATTAATAAACGAAGAGCGAACGTATCACTACAAAAGATGTATA 1850 1860 1870 1880 1890 1900 1910 1920 | | | | | | | | B73 TATAGTGTACTGCCGATGACCTGGCCGCCCAAAAAATCCAAATCATTACTTCATGATTTTTCTTTTACTTGTTTTGCTTT W22 TATAGTGTACTGCCGATGACCTGGCCGCCCAAAAAATCCAAATCATTACTTCATGATTTTTCTTTTACTTGTTTTGCTTT Teo TATAGTGTACTGCCGATGACCTGGCCGCCCAAAAAATCCAAATCATTACTTCATGATTTTTCTTTTACTTGTTTTGCTTT

1930 1940 1950 1960 1970 1980 1990 2000 | | | | | | | | B73 TTTCATGGCCCTGAGGTTTTTTT-ACATTTTCTTTTTTGGATTTCCTAGTTTACCAAGTTTCTGCTCGGTAGATTATATA W22 TTTCATGGCCCTGAGGTTTTTTTTACATTTTCTTTTTTGGATTTCCTAGTTTACCAAATTTCTGCTCGGTAGATTATATA Teo TTTCATGGCCCTGAGGTTTTTTTTACATTTTCTTTTTTGGATTTCCTAGTTGACCAAGTTTATGCTCGGTAGATTATATA 2010 2020 2030 2040 2050 2060 2070 2080 | | | | | | | | B73 TACTCCATCGGTCTCAAATAAGTATTCGTTTTAACTCTTATTTTTATGTCTATATATTTAAATAAATGATGATGAATCTA W22 TACTCCATCGGTCTCAAATAAGTATTCGTTTTAACTCTTATTTTTATGTCTATAT--TTAAATAAATGATGATGAATCTA Teo TACTCCATCGGTCTCAAATAAGTATTCGTTTTTACTCTTATTTTTATATCTATAT--TTAAATAAATGATGATGAATCTA 2090 2100 2110 2120 2130 2140 2150 2160 | | | | | | | | B73 GACACATATACAAAACACATATATTAATTATTGTATGAACCTATTAAAAAGCTAAAACAGATTTTATCATATTCCACAAA W22 GACACATATACAAAACACATATATTAATTATTGTATGAACCTATTAAAAAGCTAAAACAGATTTTATCATATTCCAGAAA Teo GACACATATACAAAACACATATATTAATTATTGTATGAACCTATTAAAAAGCTAAAACAGATTTTATCATATTCCACAAA 2170 2180 2190 2200 2210 2220 2230 2240 | | | | | | | | B73 CCTTAGTGACAATGAATACAAGGAAAATAAAATACTCCGCTATGCTAAAACATTATTCACGTACGTGTTCTCAAAAGAAC W22 CCTTAGTGACAATGAATACAAGGGAAATAAAATACTCCGCTATGCTAAAACATTATTCACGTACGTGTTCTCAAAAGAAC Teo CCTTAGTGACAATGAATACAAGGAAAATAAAATACTCCGCTATGCTAAAACATTATTCACGTACGTGTTCTCAAAAGAAC 2250 2260 2270 2280 2290 2300 2310 2320 | | | | | | | | B73 TAATTAAGGAGGTGTTCTTGCAAATTATACAGAATTTATGCTTAGTTTTTCTTCAATTAATTTCCTTTTTCTATCGTTGT W22 TAATTAAGGAGGTGTTCTTGCAAATTATACAGAATTTATGCTTAGTTTTTCTTCAATTAATTTCCTTTTTCTATCGTTGT Teo TAATTAAGGAGGTGTTCTTGCAAATTATACAGAATTTATGCTTAGTTTTTCTTCAATTAATTTCCTTTTTCTATCGTTGT 2330 2340 2350 2360 2370 2380 2390 2400 | | | | | | | | B73 TCTCTTGTGCATTAAATTAGTGTTCATAGTTCTTTATCCACATGGTCAACCTCTCTTCATGGAATCATGGATGGGGCGGT W22 TCTCTTGTGCATTAAATTAGTGTTCATAGTTCTTTATCCACATGGTCAACCTCTCTTCATGGAATCATGGATGGGGCGGT Teo TCTCTTGTGCATTAAATTAGTGTTCATAGTTCTTTATCCACATGGTCAACCTCTCTTCATGGAATCATGGATGGGGCGGT 2410 2420 2430 2440 2450 2460 2470 2480 | | | | | | | | B73 GATAGATAACCCATCATCATCGCCATTTCAGTGTAAACATTGCCTATAGGTTTGCAGTTGTTAAAGAAAACAACATATTA W22 GATAGATAACCCATCATCATCGCCATTTCAGTGTAAACATTGCCTATAGGTTTGCAGTTGTTAAAGAAAACAACATATTA Teo GATAGATAACCCATCATCATCGCCATTTCAGTGTAAACATTGCCTATAGGTTTGCAGTTGTTAAAGAAAACAACATATTA 2490 2500 2510 2520 2530 2540 2550 2560 | | | | | | | | B73 CATCTAGCTTATTCATTATTCATCATGAAGTTCTTCCTTTCTAGCTAGCTTATTCATTCTAACTTCTCGCGCCGTAGTTA W22 CATCTAGCTTATTCATTATTCATCATGAAGTTCTTCCTTTCTAGCTAGCTTATTCATTCTAACTTCTCGCGCCGTAGTTA Teo CATCTAGCTTATTCATTATTCATCATGAAGTTCTTCCTTTCTAGCTAGCTTATTCATTCTAACTTCTCGCGCCGTAGTTA 2570 2580 2590 2600 2610 2620 2630 2640 | | | | | | | | B73 CAGGGAATCCATACATCAAAGGGTACAGATTCCTTCTTGTACTATTTATGTTTGCGTCTAGCATGCATACTAGTAGAGAA W22 CAGGGAATCCATACATCAAAGGGTACAGATTCCTTCTTGTACTATTTATGTTTGCGTCTAGCATGCATACTAGTAGAGAA Teo CAGGGAATCCATACATCAAAGGGTACAGATTCCTTCTTGTACTATTTATGTTTGCGTCTAGCATGCATACTAGTAGAGAA 2650 2660 2670 2680 2690 2700 2710 2720 | | | | | | | | B73 ATTATGGGCTTCAGTCCTTGTTGGGATCTGGATTTTCTCAACCGGGAATAATTAGAACAAGTCTCTCGTATGTGCGATTC W22 ATTATGGGCTTCAGTC-TTGTTGGGATCTGGATTTTCTCAACCGGGAATAATTAGAACAAGTCTCTCGTATGTGCGATTC Teo ATTATGGGCTTCAGTCCTTGTTGGGATCTGGATTTTCTCAACCGGGAATAATTAGAACAAGTCTCTCGTATGTGCGATTC 2730 2740 2750 2760 2770 2780 2790 2800 | | | | | | | | B73 GTAGGACTCAAACCCAAACCTCATGTATTGTCTCGCGCTTAGCTTTATTTTAACTATATGGATAATATGTTTTTCTTTTA W22 GTAGGACTCAAACCCAAACCTCATGTATTGTCTCGCGCTTAGCTTTATTTTAACTATATGGATAATATGTTTTTCTTTTA Teo GTAGGACTCAAACCCAAACCTCATGTATTGTCTCGCGCTTAGCTTTATTTTAACTATATGGATAATATGTTTTTCTTTTA 2810 2820 2830 2840 2850 2860 2870 2880 | | | | | | | | B73 AACTGACCCAGAAAAGACCCTTGAGTCTCGTTTGATAATGTTGGACTAAAGATCCTCCAACGTCCAGCTCTTATTAGTCC W22 AACTGACCCACAAAAGACCCTTGAGTCTCGGTTGATAATGTTGGACTAAAGATCCTCCAACGTCCAGCTCTTATTAGTCC Teo AACTGACCCACAAAAGACCCTTGAGTCTCGGTTGATAATGTTGGACTAAAGATCCTCCAACGTCCAGCTCTTATTAGTCC

2890 2900 2910 2920 2930 2940 2950 2960 | | | | | | | | B73 TAAAAAAGTCAAACCAGGACTGATATGCTTACTGGAGACGTGTTCTTTGTCGAGTGCCTCAGACACTCGACAAACGTTTT W22 TAGAAAAGTCTAACCAGGACTGATATGCTTACTGGAGACGTGTTCTTTGTCGAGTGCCTCAGACACTCGACAAACGTTTT Teo TAGAAAAGTCTAACCAGGACTGATATGCTTACTGGAGACGTGTTCTTTGTCGAGTGCCTCAGACACTCGACAAACGTTTT 2970 2980 2990 3000 3010 3020 3030 3040 | | | | | | | | B73 ACCGAGTGCCGCACTCTGCAAAGAGATCTAG-TAAAAAAATCATCGGCGAAAGATTCTTTGTCGAGTGCTTTCTATCGGG W22 ACCGAGTGCCGCACTCTGCAAAGAGATCTAGGTAAAAAAATCATCGGCGAAAGATTCTTTGTCGAGTGCTTTCTATCGGG Teo ACCGAGTGCCGCACTCTGCAAAGAGATCTAGGTAAAAAAATCATCGGCGAAAGATTCTTTGTCGAGTGCTTTCTATCGGG 3050 3060 3070 3080 3090 3100 3110 3120 | | | | | | | | B73 CACTCGGTAAAGACTTTGCCGAGTGCTGAATACATACTCGGCAAAGAAAAGGTGTCATGACAATATGTGCCATGTATGGA W22 CACTCGGTAAAGACTTTGTCGAGTGCTGAATACATACTCGGCAAAGAAAAGGTGTCATGACAATATGTGCCATGTATGGA Teo CACTCGGTAAAGACTTTGCCGAGTGCTGAATACATACTCGGCAAAGAAAAGGTGTCATGACAATATGTGCCATGTATGGA 3130 3140 3150 3160 3170 3180 3190 3200 | | | | | | | | B73 GTATTTGTCGAGTATCTAGGTGTGTCACTCGGCAAAGCAAGGTTTGAAATGGCCCACGCAACGGATTTATTGTCGAGTGC W22 GTCTTTGTCGAGTATCTAGGTGTGTCACTCGGCAAAGTAAGGTTTGAAATGGCCCACGCAACGGATTTATTGTCGAGTGC Teo GTCTTTGTCGAGTATCTAGGTGTGTCACTCGGCAAAGCAAGGTTTGAAATGGCCCACGCAACGGATTTATTGTCGAGTGC 3210 3220 3230 3240 3250 3260 3270 3280 | | | | | | | | B73 TATGGCCTGCCGCACTCGGCAAAAGGTGGCGACTTTGCTGAGTGCCTTGGCGGCACGGGATAAAGCAAGTTTCTAGGTGC W22 TATGGCCTGCCGCACTCGGCAAAAGGTGGCGACTTTGCTGAGTGCCTTGGCGGCACTGGATAAAGCAAGTTTCTAGGTGC Teo TATGGCCTGCCGCACTCGGCAAAAGGTGGCGACTTTGCTGAGTGCCTTGGCGGCACTGGATAAAGCAAGTTTCTAGGTGC 3290 3300 3310 3320 3330 3340 3350 3360 | | | | | | | | B73 CAAGGCATATTCATTTTTCCATATTTCATGTTATTGAGTATCATTTTTCCATA--------------CTCGATAAGTGTG W22 CAAGGCATATTCATTTTTCCATATTTCATGTTATTGAGTATCATTTTTCCATATTCATTTTTCCATACTCGATAAGTGTG Teo CAAGGCATATTCATTTTTCCATATTTCATGTTATTGAGTATCATTTTTCCATATTCATTTTTCCATACTCGATAAGTGTG 3370 3380 3390 3400 3410 3420 3430 3440 | | | | | | | | B73 CAAGAAAAATTGATATGGCCTGCCGCACTCGGCAAAGGTGGTGACTTTGTTGAGTGCCTTGGCTCTGGCACTCGATAAAG W22 CAAGAAAAATTGATATGGCCTGCCGCACTCGGCAAAGGTGGTGACTTTGTTGAGTGCCTTGGCTCTGGCACTCGATAAAG Teo CAAGAAAAATTGATATGGCCTGCCGCACTCGGCAAAGGTGGTGACTTTGTTGAGTGCCTTGGCTCTGGCACTCGATAAAG 3450 3460 3470 3480 3490 3500 3510 3520 | | | | | | | | B73 CAAGTTTCTAGGTGTCAAGGCATATCCATTTTTCCATATTCATGTTATTGAGTATCATTTTTCATTCATTTTTTCTATTA W22 CAAGTTTCTAGGTGTCAAGGCATATCCATTATTCCATATTCATGTTATTGAGTATCATTTTTCATTCATTTTTTATATTA Teo CAAGTTTCTAGGTGTCAAGGCATATCCATTTTTCCATATTCATGTTATTGAGTATCATTTTTCATTCATTTTTTCTATTA 3530 3540 3550 3560 3570 3580 3590 3600 | | | | | | | | B73 TTCGAATGACTTGTAGTTCAGATTTGAATTATTCGAATACATTTAATTTAATAAAAAAAATTACTAGATATATCAAACAC W22 TTCGAATGACTTGTAGTTCAGATTTGAATTATTCGAAGACATTTAATTTAATAAAAAAA-TTACTAGATATATCAAACAC Teo TTCGAATGACTTGTAGTTCAGATTTGAATTATTCGAAGACATTTAATTTAATAAAAAAA-TTACTAGATATATCAAACAC 3610 3620 3630 3640 3650 3660 3670 3680 | | | | | | | | B73 TTATAGAAATGTGCTAAAATTGAACATATAGTTCCATATACTCTCACAACAGCACTGTACACCATTTTCGGCGGCCCCTG W22 TTATAGGAATGTGCTAAAATTGAACATATAGTTCCATATACTCT------------------------------------ Teo TTATAGAAATGTGCTAAAATTGAACATATAGTTCCATATACTCT------------------------------------ 3690 3700 3710 3720 3730 3740 3750 3760 | | | | | | | | B73 TTATTTTCGGCGGCTAGGGGGGGCCGCCGAAAATAGTGGGTTATTTTCGGCGGTCTGACACGACCGCCGAAAGTAGTGCT W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 3770 3780 3790 3800 3810 3820 3830 3840 | | | | | | | | B73 ATTTTCGGCGGCCTCTGACACCAGCCGCCGAAAATAAGGCTATTTTCGGCGGCCTCTGACACCAGCCGCCGAAAATAAGG W22 -------------------------------------------------------------------------------- Teo --------------------------------------------------------------------------------

3850 3860 3870 3880 3890 3900 3910 3920 | | | | | | | | B73 CTATTTTCGGCGGCCTCTGACCTGGCCGCCGAAAATGAGAAATTTAAAACTGTCGGCCTTCTCCTATCTTCTTTCTCTCC W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 3930 3940 3950 3960 3970 3980 3990 4000 | | | | | | | | B73 GCGCTCTCTCTCTCACCCGACGCCGCCGCGCTCTCTCTCTCACCCGACGCCGCGCCGTGTCTCTGCCCACGCCGCCGCGC W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4010 4020 4030 4040 4050 4060 4070 4080 | | | | | | | | B73 CGCCGCCCGCGCCGCCGCCGCCGAAGGAAGCTGCGCGCACCGCCGTCCGCCGCCGTCCGTCCCCGCAGTGGTCGTCCGTC W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4090 4100 4110 4120 4130 4140 4150 4160 | | | | | | | | B73 GGCCCGCCATTCCACCGCCGTCGTCGAGCCGCCGCATCGAGAGAGGTAATTTTTTTTAGTATTTTATTTCTTATTTTCGA W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4170 4180 4190 4200 4210 4220 4230 4240 | | | | | | | | B73 CGGCTGTTATTTAGATGCCGCCGAAATGGCTTTTCTCCAACAGCAATCAGATTACATGGCTGCTTACAACGCACAGGCGC W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4250 4260 4270 4280 4290 4300 4310 4320 | | | | | | | | B73 AACAAGCAATGAACGTGAGTGTCTTATTTATTCTCAACACGCTCGATATTGGTTCTATAACTAATATATTATATTTGCAA W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4330 4340 4350 4360 4370 4380 4390 4400 | | | | | | | | B73 CAGTCTTGGTTTCCACAGCAGGCACAGCAGCAACCATTTGTTTTCCCTCAATTTCAGCCGCCGATGCCTCAGTGGGGATT W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4410 4420 4430 4440 4450 4460 4470 4480 | | | | | | | | B73 ACATGCTCCGCCACCTCCACCTCCACCTCAGGTTATTCAATTTAGTTAAGACTTGTCGTTTGTATCAACCTAATTGTCAA W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4490 4500 4510 4520 4530 4540 4550 4560 | | | | | | | | B73 ATCTTTACTAAACTTGATTTTGTAGGGATCCGGGGTCATGGGCCACAACACGCCACCACCGGTCATACCAGCACCAGGAG W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4570 4580 4590 4600 4610 4620 4630 4640 | | | | | | | | B73 GAGCTTATGCAGGGGAGGGAGCTTATGCAGGAGAGGTTAGTTTGAGAAACAATTTGTTTGAATAGTCGTTATACATGTAA W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4650 4660 4670 4680 4690 4700 4710 4720 | | | | | | | | B73 CTGTAGCCGTTATTAATGTCATCTTTTTTTTAGGATCCAAACCCGTTACACGACTTCGTCGACCAGTTATTGGCTTCTGG W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4730 4740 4750 4760 4770 4780 4790 4800 | | | | | | | | B73 AGGTAGTGGACACAACTCCAACGACCCCAATGTGTGATTAAGTTAGCTTTGTGCCGCACTGTGTTGTAATGAACTATTTG W22 -------------------------------------------------------------------------------- Teo --------------------------------------------------------------------------------

4810 4820 4830 4840 4850 4860 4870 4880 | | | | | | | | B73 TGGACTTTATGTTTGTATGGACTATTTGTGGACTTTATATTTGTATGGACTCAAGTTTTATGTTATGAACTCAAGTATGA W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4890 4900 4910 4920 4930 4940 4950 4960 | | | | | | | | B73 ATTTTCTGTTATTGTTTGGAATTTCTGTGATTGTATGAATTTTGTATAAAATTCTGTTATTTTTCGTTTTTTTCAATTTT W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 4970 4980 4990 5000 5010 5020 5030 5040 | | | | | | | | B73 TTTTGCTCTGAAATTAGTAATTTTCGGCGGCCCCCTGGCCGCCGAAAATAAGGGTTTTATTTTCGGCAGTTTTTTATTTT W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 5050 5060 5070 5080 5090 5100 5110 5120 | | | | | | | | B73 CGGCGGCCGGCTCCCTGGCCGCCGAAAGTAAAGCCTTATTTTCGGCCAGTTTTTTTTGGCGGCCAGAAGCCGCCGAAAAT W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 5130 5140 5150 5160 5170 5180 5190 5200 | | | | | | | | B73 AAGCCTTTTGCCGCCGAAAATAGCTTATTTTCGGCGGGAACTGGCTTATTTTCGGCGGCTTCTGGCCGCCGAAAATGACT W22 -------------------------------------------------------------------------------- Teo -------------------------------------------------------------------------------- 5210 5220 5230 5240 5250 5260 5270 5280 | | | | | | | | B73 GTAGCTGTTGTAGTGTCTAGGAACATGATAGAAAAATGTTGGAGGAAAAAGAAAAAAATAAAGTCACTTTGTCGAGTGTC W22 ------------------AGGAACACGATAGAAAAATGTTGGAGGAAAAAGAAAAAAATAAAGTCACTTTGCCGAGTGTC Teo ------------------AGGAACACGATAGAAAAATGTTGGAGGAAAAAGAAAAAAATAAAGTCACTTTGCCGAGTGTC 5290 5300 5310 5320 5330 5340 5350 5360 | | | | | | | | B73 AACAAAGGCACTCCCAAGCTGACCCTTTGCCGAGTGTCTGGAGGTAGCACTGGTGCCTTTGCCGAGTGCCAGCCTAAAAC W22 AACAAAGGCACTCCCAAGCTGATCCTTTGCCGAGTGTCTGGAGGTAGCACTGGTGCCTTTGCCGAGTGCCAGCCTAAAAC Teo AACAAAGGCACTCCCAAGCTGATCCTTTGCCGAGTGTCTGGAGGTAGCACTGGTGCCTTTGCCGAGTGCCAGCCTAAAAC 5370 5380 5390 5400 5410 5420 5430 5440 | | | | | | | | B73 ACTTGGCAGACTTAACAATCGTGAGCTGTCGTCGGCTGCTTATGGCGTTTTTCCGAGTGACAGGTTTTGCGAGTGTCTAA W22 ACTTGGCAGACTTAACAATCGTGAGCTGTCGTCGGCTGCTTATGGCGTTTTTCCGAGTGACAGGTTTTGCGAGTGTCTAA Teo ACTTGGCAGACTTAACAATCGTGAGCTGTCGTCGGCTGCTTATGGCGTTTTTCCGAGTGACAGGTTTTGCGAGTGTCTAA 5450 5460 5470 5480 5490 5500 5510 5520 | | | | | | | | B73 CACTAGGCAAAATAGGTGTCTGAATATGCCTCGGCAAACCACCTTTTTATCGAGTGTTTTGTTTTGCCGAGTGCGACACT W22 CACTAGGCAAAATAGGTGTCTGGATATGGCTCAGCAAACCACCTTTTTGTCGAGTGTTTTGTTTTGCCGAGTGCGACACT Teo CACTAGGCAAAATAGGTGTCTGGATATGGCTCGGCAAACCACCTTTTTGTCGAGTGTTTTGTTTTGCCGAGTGCGACACT 5530 5540 5550 5560 5570 5580 5590 5600 | | | | | | | | B73 CGACTGACTTTGTCGAGTGCCTGACTTTTCATAATCGACAAATGTTTTAGAACTTTGCTAAATACCGCGTTTCGGATAGT W22 CGGCTGACTTTGTCGAGTGCCTGACTTTTCATAATCGACAAATGTTTTAGAACTTTGCTAAATACCGCGTTTCGGATAGT Teo CGGCTGACTTTGTCGAGTGCCTGACTTTTCATAATCGACAAATGTTTTAGAACTTTGCTAAATACCGCGTTTCGGATAGT 5610 5620 5630 5640 5650 5660 5670 5680 | | | | | | | | B73 GGAAGGTCGTTTCTCTAGTGGCATAATGAAGTATATAGATCCAATTGGTCATGTGCATGCTGAGCCTTGTTGCGTGAAAC W22 GGAAGGTCGTTTCTCTAGTGGCATAATGAAGTATATAGATCCAATTGGTCATGTGCATGCTGAGCCTTGTTGCGTGAAAC Teo GGAAGGTCGTTTCTCTAGTGGCATAATGAAGTATATAGATCCAATTGGTCATGTGCATGCTGAGCCTTGTTGCGTGAAAC 5690 5700 5710 5720 5730 5740 5750 5760 | | | | | | | | B73 TAAATGGCTTATTGTAATCTTTCACACCGGCCACCCTTGTGTTCCGTTCTCCACATTAATTTCATCGCAACTCGTAGTAA W22 TAAATGGCTTATTGTAATCTTTCACACCGGCCACCCTTGTGTTCCGTTCTCCACATTAATTTCATCGCAACTCGTAGTAA Teo TAAATGGCTTATTGTAATCTTTCACACCGGCCACCCTTGTGTTCCGTTCTCCACATTAATTTCATCGCAACTCGTAGTAA

5770 5780 5790 5800 5810 5820 5830 5840 | | | | | | | | B73 ATACCAAAGGCCGAGGATAAAGGTAGCTAGAGTCGCAGAGATGATATACATCGATCAGTCAGTCAATCGCTGTGATGCTA W22 ATACCAAAGGCCGAGGATAAAGGTAGCTAGAGTCGCAGAGATGATATACATCGATCAGTCAGTCAATCGCTGTGATGCTA Teo ATACCAAAGGCCGAGGATAAAGGTAGCTAGAGTCGCAGAGATGATATATATCGATCAGTCAGTCAATCGCTGTGATGCTA 5850 5860 5870 5880 5890 5900 5910 5920 | | | | | | | | B73 TGCATGCATATCTACTTGTGCAGTGTCGATACATATTTATACTATAGCTATATGAGCAGTGGGCGGATCTCTAGAAATTT W22 TGCATGCATATCTACTTGTGCAGTGTCGATACATATTTATACTATAGCTATATGAGCAGTGGGCGGATCTCTAGAAATTT Teo TGCATGCATATCTACTTGTGCAGTGTCGATACATATTTATACTATAGCTATATGAGCAGTGGGCGGATCTCTAGAAATTT 5930 5940 5950 5960 5970 5980 5990 6000 | | | | | | | | B73 AGTGTGCGTTTTGTTGTAATGATAGAACGGGGTGGAATAGAACGATCACTTAAATAAGTTTGTTTGAGTTGAGACAGTAC W22 AGTGTGCATTTTGTTGTAATGATAGAATGGGGTGGAATAGAACGATCACTTAAATAAGTTTGTTTGAGTTGAGACAGTAC Teo AGTGTGCGTTTTGTTGTAATGATAGAACGGGGTGGAATAGAACGATCACTTAAATAAGTTTGTTTGAGTTGAGACAGTAC 6010 6020 6030 6040 6050 6060 6070 6080 | | | | | | | | B73 TATCCTACTCCCGCAACCAAACACATCTTTAGAGAACCTCTAGCTAGAGGCTAGCTCTTCCCTTTTTCATTTTCATAAAA W22 TATCCTACTCCCGCAACCAAACACATCTTTAGAGAACCTCTAGCTAGAGGCTAGCTCTTCCCTTTTTCATGTTCATAAAA Teo TATCCTACCCCCGCAACCAAACACATCTTTAGAGAACCTCTAGCTAGAGGCTAGCTCTTCCCTTTTTCATTTTCATAAAA 6090 6100 6110 6120 6130 6140 6150 6160 | | | | | | | | B73 GCTAGATAAAATGGGCAAAAGGAGGGGTCCTATATATTGTCGCGGAAGCAGTAGAGGGGGGGGGGGGGGGGG---CTTGA W22 GCTAGATAAAATGGGCAAAAGGAGGGGTCCTATATATTGTCGCGGAAGCAGTAGAGGGGGGGGGGGGGGGGGGGGCTTGA Teo GCTAGATAAAATGGGCAAAAGGAGGGGTCCTATATATTGTCGCGGAAGCAGAAGAGGGGGGGGGGGGGGGGGGGGCTTGA 6170 6180 6190 6200 6210 6220 6230 6240 | | | | | | | | B73 GCGAGATCATCAT------------------------------------------------------------------- W22 GCGAGATCATCATGGGTATGTTTGGTTCAGCTTTTTTCTGACCAGCTTTTCTGAAAATCTGGTTGTGGGGAGAATCTGGC Teo GCGAGATCATCAT------------------------------------------------------------------- 6250 6260 6270 6280 6290 6300 6310 6320 | | | | | | | | B73 -------------------------------------------------------------------------------- W22 TGTGGAGAGAATCTGAGTATTATTACGATTACGTGTGGAGGAAGATAAAGCTGTTCATAAGGCTCAGGATCTAGAAAGTG Teo -------------------------------------------------------------------------------- 6330 6340 6350 6360 6370 6380 6390 6400 | | | | | | | | B73 -------------------------------------------------------------------------------- W22 ACGGATTCCTACTATTACAATGACTCAACCGATTATGTGTTTATATTGATTTTGGATGGTTTTTGCCGCAACGAATTTTA Teo -------------------------------------------------------------------------------- 6410 6420 6430 6440 6450 6460 6470 6480 | | | | | | | | B73 -------------------------------------------------------------------------------- W22 TAGAAGCTGGCTGAAAAACTGAACGTTTGGCAGTCCGCAGCAGCTTTTGGTGGCCAGAAGCTGCCAGAAGCCGAAACAAA Teo -------------------------------------------------------------------------------- 6490 6500 6510 6520 6530 6540 6550 6560 | | | | | | | | B73 ----------ATCTTTCGCAAGAGATCTTTGCCATCGCTGAAGTCTCTGAACGAAGTGGCAGTACTTTTTCTCCAGCTCC W22 CATGGCCCATATCTTTCGCAAGAGATCTTTGCCATCGCTGAAGTCTCTGAACGAAGTGGCAGTACTTTTTCTCCAGCTCC Teo ----------ATCTTTCGCAAGAGATCTTTGCCATCGCTGAAGTCTCTGAACGAAGTGGCAGTACTTTTTCTCCAGCTCC 6570 6580 6590 6600 6610 6620 6630 6640 | | | | | | | | B73 AATGCCGTCTCCCCCTCTACTAGCCAAAGATAAAAAGGAGGTGTTTACTGGTCGATCTTTGCATTGCAAGCCCGCCGTGC W22 AATGCCGTCTCCCCCTCTACTAGCCAAAGATAAAAAGGAGGTGTTTACTGGTCGATCTTTGCATTGCAAGCCCGCCGTGC Teo AATGCCGTCTCCCCCTCTACTAGCCAAAGATAAAAAGGAGGTGTTTACTGGTCGATCTTTGCATTGCAAGCCCGCCGTGC 6650 6660 6670 6680 6690 6700 6710 6720 | | | | | | | | B73 GAAAGTGGCTTCTGCATGAATTGTCACTATCTCCTTGCGTCTTTCTGGTCTGCAAAACTTTGTCCTAAATATATTCTTTC W22 GAAAGTGGCTTCTGCATGAATTGTCACTATCTCCTTGCGTCTTTCTGGTCTGCAAAACTTTGTCCTAAATATATTCTTTC Teo GAAAGTGGCTTCTGCATGAATTGTCACTATCTCCTTGCGTCTTTCTGGTCTGCAAAACTTTGTCCTAAATATATTCTTTC

6730 6740 6750 6760 6770 6780 6790 6800 | | | | | | | | B73 AGCGTTTTGTGTTTGTGTCAGAGACGATGGACCATGCATACATACATGGAGCAGAAAATATATATTTATATATATAGCTG W22 AGCGTTTTGTGTTTGTGTCAGAGACGATGGACCATGCATACATACATGGAGCAGAAAATATATATTTATATATATAGCTG Teo AGCGTTTTGTGTTTGTGTCAGAGACGATGGACCATGCATACATACATGGAGCAGAAAATATATATTTATATATATAGCTG 6810 6820 6830 6840 6850 6860 6870 6880 | | | | | | | | B73 CACGCGCGCTAATGGGTACGGTGATGAGTTCTCTACAAATCAGATCACACATCACATCGCACAATGTGTGTGTGTGTGCA W22 CACGCGCGCTAATGGGTACGGTGATGAGTTCTCTACAAATCAGATCACACATCACATCGCACAAT--------------- Teo CACGCGCGCTAATGGGTACGGTGATGAGTTCTCTACAAATCAGATCACACATCACATCGCACAAT--------------- 6890 6900 6910 6920 6930 6940 6950 6960 | | | | | | | | B73 AGATATATGAAGTGTGACAGATTTAACTCATCGTCCCCTCATGGAGCACGTACACACAATCATTTGTAGGGCATACACTC W22 ----ATATGAAGTGTGACAGATTTAACTCATCGTCCCCTCATGGAGCACGTACACACAATCATTTGTAGGGCATACACTC Teo ----ATATGAAGTGTGACAGATTTAACTCATCGTCCCCTCATGGAGCACGTACACACAATCATTTGTAGGGCATACACTC 6970 6980 6990 7000 7010 7020 7030 7040 | | | | | | | | B73 TCTCACTTGTTTAGCTAATACCATATGGGAGTTTGTGGAGCTCATGGGAGTTTGTGCGCACAAAAAAAGGACAGCGTGAG W22 TCTCACTTGTTTAGCTAATACCATATGGGAGTTTGTGGAGCTCATGGGAGTTTGTGCGCACAAAAAAAGGACAGCGTGAG Teo TCTCACTTGTT-AGCTAATACCATATGGGAGTTTGTGGAGCTCATGGGAGTTTGTGCGCACAAAAAA-GGACAGCGTGAG 7050 7060 7070 7080 7090 7100 7110 7120 | | | | | | | | B73 GAATCAAACAAATTAAAGTAGGCCCTTTGCATTATTGTTTGTTCAGTTTGGGTGTGCACCAACAGTGTTTGTTCAGCAGC W22 GAATCAAACAAATTAAAGTAGGCCCTTTGCATTATTGTTTGTTCAGTTTGGGTGTGCACCAACAGTGTTTGTTCAGCAGC Teo GAATCAAACAAATTAAAGTAGGCCCTTTGCATTATTGTTTGTTCAGTTTGGGTGTGCACCAACAGTGTTTGTTCAGCAGC 7130 7140 7150 7160 7170 7180 7190 7200 | | | | | | | | B73 AGAGCCCTGATAAAAGTCATGGAGCAAATAAACAGTTCCGCTGGCAGCCATATGCATGCAGGAAAAGAGAGAAGGCGTTC W22 AGAGCCCTGATAAAAGTCATGGAGCAAATAAACAGTTCCGCTGGCAGCCATATGCATGCAGGAAAAGAGAGAAGGCGTTC Teo AGA-CCCTGATAAAAGTCATGGAGCAAATAAACAGTTCCGCTGGCAGCCATATGCATGCAGGAAAAGAGAGAAGGCGTTC 7210 7220 7230 7240 7250 7260 7270 7280 | | | | | | | | B73 GAGCATATGCATCTTTATTTTACTCCTCGTTCTTCTGCAAAAAACTATATGTAAAGAGGATTTTTTT------------- W22 GAGCATATGCATCTTTATTTTACTCCTCGTTCTTCTGCAAAAAACTATATGTAAAGAGGATTTTTTTCACTACACGACGG Teo GAGCATATGCATCTTTATTTTACTCCTCGTTCTTCTGCAAAAGCCTATATGTAAAGAGGATTTTTTT------------- 7290 7300 7310 7320 7330 7340 7350 7360 | | | | | | | | B73 -------------------------------------------------------------------------------- W22 TTGATCTTTAGCGACCCTTATTTGGGACCGACGGTTGGTCGCTAAAAGTTATGGACCGATGGTTGGTCGCTAAATGCGTT Teo -------------------------------------------------------------------------------- 7370 7380 7390 7400 7410 7420 7430 7440 | | | | | | | | B73 -------------------------------------------------------------------------------- W22 TATAGCGACGGTCTGTGGGTCGCTAAAAGTCTAGAAATTTAACAACTTATGGTCGGTCGCTATAGAGTTAAGTGTGTGAC Teo -------------------------------------------------------------------------------- 7450 7460 7470 7480 7490 7500 7510 7520 | | | | | | | | B73 ---------------------------------------------CCCCTTGCTTCTGGTGTGCATCTTTTCATGCTATA W22 TGATGGTGGA??????????GTAAGTGAACTCTCGTGTAGTGTTTCCCCTTGCTTCTGGTGTGCATCTTTTCATGCTATA Teo --------------------------------------------TCCCCTTGCTTCTCGTGTGCACCTTTTCATGCTATA 7530 7540 7550 7560 7570 7580 7590 7600 | | | | | | | | B73 TATATATATATACACTTTCACGGCGATAATTTAAGTACCGTAAGCAAATGCATACTGGATTTCTTCAATAATAAATAGAC W22 TATATATATA--CACTTTCACGGCGATAATTTAAGTACCGTAAGCAAATGCATACTGGATTTCTTCAATAATAAATAGAC Teo TATATA------CACTTTCACGGCGATAATTTAAGTACCGTAAGCAAATGCATACTGGATTTCTTCAATAATAAATAGAC 7610 7620 7630 7640 7650 7660 7670 7680 | | | | | | | | B73 CAAATAATAATACTGGTGTCTCGTTTAATTTATGATCAATGTGTGCATGCAGGTGAGTGTCCCGGGTCACAGCATGCTGA W22 CAAATAATAATACTGGTGTCTCGTTTAATTTATGATCAATGTGTGCATGCAGGTGAGTGTCCCGGGTCACAGCATGCTGA Teo CTAATAATAATACTGGTGTCTCGTTTAATTTATGATCAATGTGTGCATGCAGGTGAGTGTCCCGGGTCACAGCATGCTGA

7690 7700 7710 7720 7730 7740 7750 7760 | | | | | | | | B73 ACATGGTGACAGTCCGTTGTGGGCACTGCACTAGCCTGCTGTCGGTGAACTTGAGAGGACTCATCCAATCACTCCCTGTC W22 ACATGGTGACAGTCCGTTGTGGGCACTGCACTAGCCTGCTGTCGGTGAACTTGAGAGGACTCATCCAATCACTCCCTGTC Teo ACATGGTGACAGTCCGTTGTGGGCACTGCACTAGCCTGCTGTCGGTGAACTTGAGAGGACTCATCCAATCACTCCCTGTC 7770 7780 7790 7800 7810 7820 7830 7840 | | | | | | | | B73 GTCCAGAATCACTACTCACAGGTTGCAATGAATTAATAGCTGCTAGCAACATCATATACTGATTCGTGCCGTGCAGTGCA W22 GTCCAGAATCACTACTCACAGGTTGCAATGAATTAATAGCTGCTAGCAACATCATATACTGATTCGTGCCGTGCAGTGCA Teo GTCCAGAATCACTACTCACAGGTTGCAATGAATTAATAGCTGCTAGCAACATCATA--CTGATTCGTGCCGTGCAGTGCA 7850 7860 7870 7880 7890 7900 7910 7920 | | | | | | | | B73 TGATCTGCGCATGAAATATGAAGGACAACACGTACAATAAACCTCTTCTCTTCTAACAGAGCAGCTAATATAAACCTTTT W22 TGATCTGCGCATGAAATATGAAGGACAACACGTACAATAAACCTCTTCTCTTCTAACAGAGCAGCTAATATAAACCTTTT Teo TGATCTGCGCATGAAATATGAAGGACAACACGTACAATAAACCTCTTCTCTTCTAACAGAGCAGCTAATATAAACCTTTT 7930 7940 7950 7960 7970 7980 7990 8000 | | | | | | | | B73 TTTTGTTTTTTGTCATATTGCTTCAAAACTGTAAC----CATGCATTCATGTCGACAAACGTTACTAACAACCTGTGTGA W22 TTTTGTTTTTTGTCATATTGCTTCAAAACTGTAAC----CATGCATTCATGTCGACAAACGTTACTAACAACCTGTGTGA Teo TTTTGTTTTTTGCCATATTGCTGCAAAACTGTAACGAACCATGCATTCATGTCGACAAACGTTACTAACAAACTGTGTGA 8010 8020 8030 8040 8050 8060 8070 8080 | | | | | | | | B73 GTACTGTTACTAGCTTGTTATGCATTATTTACTGTAC----TATACTGCATTGTATCTCGCATGAAAGAGTTTAAAGCTT W22 GTACTGTTACTAGCTTGTTATGCATTATTTACTGTAC----TATACTGCATTGTATCTCGCATGAAAGAGTTTAAAGCTT Teo GTACTGTTACTAGCTTGTTATGCATTATTTACTGTACGTACTATACTGCAATGTATCTCGCATGAAAGAGTTTAAAGCTT 8090 8100 8110 8120 8130 8140 8150 8160 | | | | | | | | B73 GCACACATGCATGC----TGCTAATTTGAACAAGATGCATCAGTTCTTCCTCACACCAAGAACTTGGGTCGATTACCAAA W22 GCACACATGCATGC----TGCTAATTTGAACAAGATGCATCAGTTCTTCCTCACACCAAGAACTTGGGTCGATTACCAAA Teo GCACACATGCATGCATGCTGCTAATTTGAACAAGATGCATCAGTTCTTCCTCACACCAAGAACTTGGGTCGATTACCAAA 8170 8180 8190 8200 8210 8220 8230 8240 | | | | | | | | B73 GTGTGCATGCATGGAACATATACCAATAATAACATGATGATTATATTGTGTCAATTGGCTTCAGGAGCATTTCAAGGTCC W22 GTGTGCATGCATGGAACATATACCAATAATAACATGATGATTATATTGTGTCAATTGGCTTCAGGAGCATTTCAAGGTCC Teo GTGTGCATGCATGGAACATATACCAATAATAACATGATGATTATATTGTGTCAATTGGCTTCAGGAGCATTTCAAGGTCC 8250 8260 8270 8280 8290 8300 8310 8320 | | | | | | | | B73 AAAATTTCAGCTTTACTGAGAACTATCCAGAGTATGCAGCACCACCTTCGTCTTCGAGATACCGCATGCCAACGATGTTG W22 AAAATTTCAGCTTTACTGAGAACTATCCAGAGTATGCAGCACCACCTTCGTCTTCGAGATACCGCATGCCAACGATGTTG Teo AAAATTTCAGCTTTACTGAGAACTATCCAGAGTATGCAGCACCACCTTCGTCTTCGAGATACCGCATGCCAACGATGTTG 8330 8340 8350 8360 8370 8380 8390 8400 | | | | | | | | B73 TCAGCAAAAGGTGATCTGGATCATATGCTGCACGTCCGTGGTAAGCTAGATTGAATCATATATGTGTGGAAGTAAGTTTT W22 TCAGCAAAAGGTGATCTGGATCATATGCTGCACGTCCGTGGTAAGCTAGATTGAATCATATATGTGTGGAAGTAAGTTTT Teo TCAGCAAAAGGTGATCTGGATCATATGCTGCACGTGCGTGGTAAGCTAGATTGAATCATATATGTGTGGAAGTAAGTTTT 8410 8420 8430 8440 8450 8460 8470 8480 | | | | | | | | B73 ATGAGAAACCTTTTTTT-CAGCATAAACACATGCGTGAAGAATCCAAAAGGAATTAATAAGAGCCCGTATTGAGTACTCC W22 ATGAGAAACCTTTTTTT-CAGCATAAACACATGCGTGAAGAATCCAAAAGGAATTAATAAGAGCCCGTATTGAGTACTCC Teo ATGAGAAACCTTTTTTTTCAGCATAAACACATGCGTGAAGAATCCAAAAGGAATTAATAAGAGCCCGTATTGAGTACTCC 8490 8500 8510 8520 8530 8540 8550 8560 | | | | | | | | B73 CTCCATTCCAAAATGTTATTCGTTTTAGCTCTTGGTTTTTATAACTATATTAAAATATATGACAATGAATTTAGACACAT W22 CTCCATTCCAAAATGTTATTCGTTTTAGCTCTTGGTTTTTATAACTATATTAAAATATATGACAATGAATTTAGACACAT Teo CTCCATTCCAAAATGTCATTCGTTTTAGCTCTTGATTTTTATATCTATATTCAAATATATGACAATGAATTTAGACACAT 8570 8580 8590 8600 8610 8620 8630 8640 | | | | | | | | B73 ATATAAAACATGTACATATATCAAGTATTATATGAATCTATTAATTAACTAAAATGAATGTTAATTTGGGACGGG---AT W22 ATATAAAACATGTACATATATCAAGTATTATATGAATCTATTAATTAACTAAAATGAATGTTAATTTGGGACGGG---AT Teo ATATAAAACATGTATATAAATCAATTATTATATGAATCCATTAATTAACTAAAACAAATGTTAATTTGGGACGGGGGAAT

8650 8660 8670 8680 8690 8700 8710 8720 | | | | | | | | B73 ACTAAACTAAGGCTTATTA---AGCACAATATGATTTTTTTCAGAGAAATAATGATGTGGTTGCAAAAGCTAAGAATGAT W22 ACTAAACTAAGGCTTATTA---AGCACAATATGATTTTTTTCAGAGAAATAATGATGTGGTTGCAAAAGCTAAGAATGAT Teo ACTAAACTAAGGCTTATTATTAAGCACAATATGATTTTTT-CAGAGAAATAATGACGTGGTTGCAAAAACTAAGAATGAT 8730 8740 8750 8760 8770 8780 8790 8800 | | | | | | | | B73 TCCTTTTAATATTATATTTGACTATAAATTATTTCTTGCAAATAATTCATCTCTCATTTACGTCTTGTTTTTGACAAAAA W22 TCCTTTTAATATTATATTTGACTATAAATTATTTCTTGCAAATAATTCATCTCTCATTTACGTCTTGTTTTTGACAAAAA Teo TCCTTTTAATATTATATTTGACTTTAAATTATTTCTTGCAAATAATTCATCTCTCGTTTACGTCTTGTTTTTGACAAAAA 8810 8820 8830 8840 8850 8860 8870 8880 | | | | | | | | B73 GCAGAGGTAAAAGGTCATGTATATATACCATCATCACCTTGAAGTTCCTTGTGTACTATGCATGTGCTCATG-TTTTTTT W22 GCAGAGGTAAAAGGTCATGTATATATACCATCATCACCTTGAAGTTCCTTGTGTACTATGCATGTGCTCATG-TTTTTTT Teo GCATAGGTAAAAAGCCATGTATATATACCATCATCACCTTGAAGTTCCTTGTGTACTATGCATGTGCTCATGCTTTTTTT 8890 8900 8910 8920 8930 8940 8950 8960 | | | | | | | | B73 TGCTTTGTTTCTACTCTTAATGTTGAAAATACAACTTAAAATATGAAAATACACTGCAAAATTGAATTTTCTTGCGAGTA W22 TGCTTTGTTTCTACTCTTAATGTTGAAAATACAACTTAAAATATGAAAATACACTGCAAAATTGAATTTTCTTGCGAGTA Teo TGCTTTGTTTCTACTCTTAAT---------------TAAAATATGAAAATACACTGCAAAATTGAATTTTCTTTCCACTA 8970 8980 8990 9000 9010 9020 9030 9040 | | | | | | | | B73 AAATTAATACGATGAAGTTTAGTAACCCTATTATGAAGTGTTGGGTAAAATTCATATAGTTTCCAAAAGTGAGTAAACCA W22 AAATTAATACGATGAAGTTTAGTAACCCTATTATGAAGTGTTGGGTAAAATTCATATAGTTTCCAAAAGTGAGTAAACCA Teo AAATTAATACAATGAAGTTTAGTAACCCTATTATGAAGTGTTGGGTAAAATTCATATAGTTTCCAAAAGTGAGTAAACCA 9050 9060 9070 9080 9090 9100 9110 9120 | | | | | | | | B73 AACATAGATAAATGTGTGCTTATAGTATTCTCTAAAAATAAGACTAGCTAATAAATATTCCAAATTCATATGTATACTTC W22 AACATAGATAAATGTGTGCTTATAGTATTCTCTAAAAATAAGACTAGCTAATAAATATTCCAAATTCATATGTATACTTC Teo AACATAGATAAATGTGT-CTTATAGTATTCTCTGAAAATAAGACTAGCCGATAAATATTCCGAATTCATATGTATACTTC 9130 9140 9150 9160 9170 9180 9190 9200 | | | | | | | | B73 ATTGCTTCATGCTAGCTCTTCTAATTCTGAAACCGTTTTTAAATATAAACCGAACGGTTTTCCCAAATGTTCAGAATCTC W22 ATTGCTTCATGCTAGCTCTTCTAATTCTGAAACCGTTTTTAAATATAAACCGAACGGTTTTCCCAAATGTTCAGAATCTC Teo ATTACTTCATGCTAGCTCTTCTAATTCTGAAACCGTTGTTTAATATAAACCGAACGGTTTTCCCAAATGTTTAGAATTTT 9210 9220 9230 9240 9250 9260 9270 9280 | | | | | | | | B73 CCTTGTTGGTTGGTGTGTGAGGTGTTCATGATGGGGTTTCATGTGGCCTTCACAGTACACTTTGCAATTGTATATTAACT W22 CCTTGTTGGTTGGTGTGTGAGGTGTTCATGATGGGGTTTCATGTGGCCTTCACAGTACACTTTGCAATTGTATATTAACT Teo CCTTGTTGGTTGGTGTGTGAGGTGTTCATGATGGGGTTTCATGTGTCCTTCACAGTACACTTTGCAATTGTATATTAACT 9290 9300 9310 9320 9330 9340 9350 9360 | | | | | | | | B73 ATAATTCATCCATACAAGAGTGTTTTCAGCAAAGAGGATTTTGTAAAATATGTATATCTATGGTGTGTATGTCCTAATAA W22 ATAATTCATCCATACAAGAGTGTTTTCAGCAAAGAGGATTTTGTAAAATATGTATATCTATGGTGTGTATGTCCTAATAA Teo ATTATTCAACCATACAAGAGTGTTTTCAGCAAAGAGGATTTTGTAAAATATGTATATCTATGGTGTGTATGTCCTAATAA 9370 9380 9390 9400 9410 9420 9430 9440 | | | | | | | | B73 CTTCATGTGTCTGGTCTTTGTAATAAACAACAGCTCCAGAGAAGAGGCAGCGTGTTCCTTCAGCATATAACAGATTTATT W22 CTTCATGTGTCTGGTCTTTGTAATAAACAACAGCTCCAGAGAAGAGGCAGCGTGTTCCTTCAGCATATAACAGATTTATT Teo CTTCATGTGTCTGGTCTTTGTAATAAACAACAGCTCCAGAGAAGAGGCAGCGTGTTCCTTCAGCATATAACAGATTTATT 9450 9460 9470 9480 9490 9500 9510 9520 | | | | | | | | B73 AAGTGAGTTGGACTAAGTCTAAGTTCATTT-CTATTTTACATTCTCTCAAATTTAAATTGTAGACCGCCATTTTAGTTTT W22 AAGTGAGTTGGACTAAGTCTAAGTTCATTT-CTATTTTACATTCTCTCAAATTTAAATTGTAGACCGCCATTTTAGTTTT Teo AAGTGAGTTGGAC-CAGTCTAAGTTCATTTTCTATTTTACATTCTCTCAAATTTAAATTGTAGACC---ATTTTAGTTTT 9530 9540 9550 9560 9570 9580 9590 9600 | | | | | | | | B73 GT-TTAAGTCAAACTTATTTAACATTAACCAGATTTATGAAAAAAGTATGTTAGCATCTACAATCAAACAAATGCACTAT W22 GT-TTAAGTCAAACTTATTTAACATTAACCAGATTTATGAAAAAAGTATGTTAGCATCTACAATCAAACAAATGCACTAT Teo GTCTTAAGTCAAACTTATTTAACATTAACCAGATTTATGAAAAAAGTATGTTAGCATCTACAATCAAACAAATGCACTAT

9610 9620 9630 9640 9650 9660 9670 9680 | | | | | | | | B73 TAAAATACATTCCATGCTGTTACTAATGGATATCGTTTTATGAAAATATATTATATGATGTATCTAGTGAAATTTGTTTG W22 TAAAATACATTCCATGCTGTTACTAATGGATATCGTTTTATGAAAATATATTATATGATGTATCTAGTGAAATTTGTTTG Teo TAAAATATATTCCATGTTGTTACTAATGGATATCGTTTTATCATAATATATTATATGATGTATCTAGTGAAATTTGTTTG 9690 9700 9710 9720 9730 9740 9750 9760 | | | | | | | | B73 ATACTATAGATGATTGTGCGATTTTTTTATAAATTTGATCAAAGTTAGAGAATCTTTGAGTTAGGACAAAACTAAAACAA W22 ATACTATAGATGATTGTGCGATTTTTTTATAAATTTGATCAAAGTTAGAGAATCTTTGAGTTAGGACAAAACTAAAACAA Teo ATATTATAGCTGATTGTGCAGTTTTTT-ATAAATTTGATCAAAGTTACAGAATCTTTGAGTTAGGACAAAACTAAAGCAA 9770 9780 9790 9800 9810 9820 9830 9840 | | | | | | | | B73 TATATATTTTGGAATGAAGGGTGTACGTATGTTGTAGTATGGTTTATGTCTAGGTATAAACAATGTAGTTTTATATAAAC W22 TATATATTTTGGAATGAAGGGTGTACGTATGTTGTAGTATGGTTTATGTCTAGGTATAAACAATGTAGTTTTATATAAAC Teo TATATACTTTGGAATGAAGGGTGTACGTATGTTGTAGTATGGTTTATGTCTAGGTATAAACAGTCTAGTTTTATATAAAC 9850 9860 9870 9880 9890 9900 9910 9920 | | | | | | | | B73 TAATATTGAAATGGTGCATGCATTGTCTTAGGGAAGAGATACGAAGGATTAAAGCAAACAACCCGGACATAAGCCATAGG W22 TAATATTGAAATGGTGCATGCATTGTCTTAGGGAAGAGATACGAAGGATTAAAGCAAACAACCCGGACATAAGCCATAGG Teo TAATATTGAAATGGTGCATGCATTGTCTTAGGGAAGAGATACGAAGGATTAAAGCAAACAACCCGGACATAAGCCATAGG 9930 9940 9950 9960 9970 9980 9990 10000 | | | | | | | | B73 GAAGCTTTCAGCACTGCAGCAAAGAAT--AAGTACATGGTTTACATAGAACAAGTGTTGTCCTATACTCAGGATA----- W22 GAAGCTTTCAGCACTGCAGCAAAGAATGTAAGTACATGGTTTACATAGAACAAGTGTTGTCCTATACTCAGGATA----- Teo GAAGCCTTTAGCACTGCAGCAAAGAATGTAAGTACATGGTTTGCAAATAACAAGTGTTGTCCTATACTCAGGATATATTG 10010 10020 10030 10040 10050 10060 10070 10080 | | | | | | | | B73 ----------------ATTATCCCATGAAAATAAATTAGATGGAAAAAAATGCAAGGAAATTGATATTGAACTTTATTAA W22 ----------------ATTATCCCATGAAAATAAATTAGATGGAAAAAAATGCAAGGAAATTGATATTGAACTTTATTAA Teo TATGTATGTAGGGATAATTATCCCATGAAAATTAATTAGATGGAAAAA--TGCAAGGAAATTGATATTGAA-TTTACTAA 10090 10100 10110 10120 10130 10140 10150 10160 | | | | | | | | B73 TAAAGATGTCTTAAAATTAATCTCATTTATCTCAAACTTTTTTAAAAA-TGTGAACAATGTTTTTTGATAATCACAAAAT W22 TAAAGATGTCTTAAAATTAATCTCATTTATCTCAAACTTTTTTAAAAA-TGTGAACAATGTTTTTTGATAATCACAAAAT Teo TAAAGATGTCTTAAAATTAATCTCATTTATCTCAAACTTTTTTAAAAAATGTGAACAATGTTTTTTGATAATCACAAAAT 10170 10180 10190 10200 10210 10220 10230 10240 | | | | | | | | B73 ACTTACCAAACATTGCTTTGTTGATTAATTGTTCATCTATAACTCCTTGATACTCTCATACCATGATTTCCTGAACTATA W22 ACTTACCAAACATTGCTTTGTTGATTAATTGTTCATCTATAACTCCTTGATACTCTCATACCATGATTTCCTGAACTATA Teo ACTTACCAAACATTGCTTTGTTGATTAATTGTTCATCTATAACTCCTTGATACTCTCATACCGTGATTTCCTGA-CTATA 10250 10260 10270 10280 10290 10300 10310 10320 | | | | | | | | B73 TTAATTTATACCATAAACATATTGTACAATCTAGATACCATATACAAACTACTTGGTAAGAACACATTTTGTTATCCAAC W22 TTAATTTATACCATAAACATATTGTACAATCTAGATACCATATACAAACTACTTGGTAAGAACACATTTTGTTATCCAAC Teo TTAATCTATATCATAAACATATTGTACAAGCTAGATACCATATACAAACTACTTGGTAAGAACACATTTTGTTATCCAAC 10330 10340 10350 10360 10370 10380 10390 10400 | | | | | | | | B73 AAAATTTGTTCAAATCATCATTGTTTGTATTAATTTCCTTTCTTCAAAGAGTTAGTTAAGAAAAACAAGAACATATATGA W22 AAAATTTGTTCAAATCATCATTGTTTGTATTAATTTCCTTTCTTCAAAGAGTTAGTTAAGAAAAACAAGAACATATATGA Teo AAAATTTTTTCAAATCATCATTGTTTGTATTAATTTCCTTTCTTCAAAGAGTTAGTTAAGAAAAACAAGAACATATATGA 10410 10420 10430 10440 10450 10460 10470 10480 | | | | | | | | B73 ATTTCTTAAAAACAATGTATAAAATGCACCCAATGCATCCATATTGATTTTGTTTTCCATTTCAGTGGGCACATTTTCCG W22 ATTTCTTAAAAACAATGTATAAAATGCACCCAATGCATCCATATTGATTTTGTTTTCCATTTCAGTGGGCACATTTTCCG Teo ATTTCTTAAAAACAATGTATAAAATGCACCCAATGCATCCATATTGATTTTGTTTTCCATTTCAGTGGGCACATTTTCCG 10490 10500 10510 10520 10530 10540 10550 10560 | | | | | | | | B73 AACATTCATTTTGGACTCGGGCCCTATGAAAGCAGCAACAAGCTTGATGAGACCATTGGTGCAACGGGTCATCCTCGGAA W22 AACATTCATTTTGGACTCGGGCCCTATGAAAGCAGCAACAAGCTTGATGAGACCATTGGTGCAACGGGTCATCCTCGGAA Teo AACATTCATTTTGGACTCGGGCCCTATGAAAGTAGCAACAAGCTTGATGAGACCATTGGTGCAACGGGTCATCCTCGGAA

10570 10580 10590 10600 10610 10620 10630 10640 | | | | | | | | B73 AATTCAAGATCCCTACTAAGACATATATCGACCTCGTTCTTTGTTTTCCTATTGTAAGTAATTTTAGATAAAAAA-CTAT W22 AATTCAAGATCCCTACTAAGACATATATCGACCTCGTTCTTTGTTTTCCTATTGTAAGTAATTTTAGATAAAAAA-CTAT Teo AATTCAAGATCCCTACTAAGACCTATATCGACATCGTTCTTTGTTTTCCTATTGTAAGTAATTTTAGATAAAAAAACTAT 10650 10660 10670 | | | B73 CTTAGTTCTGAACTAGTTTGTGAGTGTCGTACATATA W22 CTTAGTTCTGAACTAGTTTGTGAGTGTCGTACATATA Teo CTTAGTTCCGAACTAGTTTGTGAGTGTCGTGTATATA

Figure S7. Alignment of GRMZM2G085873 sequences from B73, W22 and teosinte. All six

exons are highlighted in grey. Start codon is highlighted in green while stop codon is

highlighted in red. B73_Spm insertion is highlighted in yellow while W22_Spm insertion is

highlighted in blue. Note that W22_Spm insertion is not completely sequenced due to the

challenge in sequencing through terminal inverted repeats of a transposable element without an

appropriate W22 bacterial artificial chromosome.

Figure S8. Phylogenetic tree for YABBY gene family. Posterior probabilities are labelled on

each node. ZmYAB2.1 falls into YAB2 clade and is highlighted in bold.

Figure S9. Linkage disequilibrium (LD) plots of SNPs from ZmYAB2.1 exon 1 region, 34 kb

upstream region, 68 kb upstream region, and five other randomly selected genomic regions from

publicly available maize 55k SNP Chip dataset (Cook et al. 2012). Note that only SNPs from

maize landraces listed in Table S2 are used here. R2 is shown on the bottom left while P-values

are shown on the top right. Both R2 and P-values use the same color scale, and P-value of 1.5e-5

corresponds to Bonferroni-corrected P-value of 0.05. There is little to no LD between

ZmYAB2.1 exon 1 region and 34 kb upstream region, suggesting that there is no power to detect

any signature of selection on the upstream regulatory region of ZmYAB2.1.

Table S1. Markers used in fine-mapping etb1.2.

Marker Type AGP_v2 Position

Polymorphism Primers (5’-3’)

umc2047 SSR 258383492 Small (T); Large (M) (F) GACAGACATTCCTCGCTACCTGAT (R) CTGCTAGCTACCAAACATTCCGAT

GBS_81 GBS 258383884 SNP -

GBS_82 GBS 258383890 SNP -

GBS_83 GBS 258383911 SNP -

GBS_84 GBS 258386943 SNP -

GBS_85 GBS 258387379 SNP -

GBS_86 GBS 258387646 SNP -

GBS_87 GBS 258397535 SNP -

GBS_88 GBS 258427527 SNP -

GBS_89 GBS 258437034 SNP -

GBS_90 GBS 258437091 SNP -

GBS_91 GBS 258441125 SNP -

GBS_92 GBS 258441270 SNP -

GBS_93 GBS 258442518 SNP -

GBS_94 GBS 258442582 SNP -

GBS_95 GBS 258442796 SNP -

GBS_96 GBS 258447918 SNP -

GBS_97 GBS 258447921 SNP -

GBS_98 GBS 258448383 SNP -

GBS_99 GBS 258448441 SNP -

GBS_100 GBS 258448583 SNP -

GBS_101 GBS 258521637 SNP -

GBS_102 GBS 258551646 SNP -

GBS_103 GBS 258552796 SNP -

GBS_104 GBS 258553443 SNP -

GBS_105 GBS 258651730 SNP -

GBS_106 GBS 258651762 SNP -

GBS_107 GBS 258655425 SNP -

GBS_108 GBS 258655426 SNP -

GBS_109 GBS 258656283 SNP -

GBS_110 GBS 258656358 SNP -

GBS_111 GBS 258656778 SNP -

GBS_112 GBS 258657130 SNP -

GBS_113 GBS 258657467 SNP -

GBS_114 GBS 258657767 SNP -

GBS_115 GBS 258673732 SNP -

GBS_116 GBS 258674017 SNP -

GBS_117 GBS 258681240 SNP -

GBS_118 GBS 258691831 SNP -

GBS_119 GBS 258796234 SNP -

GBS_120 GBS 258796310 SNP -

LEK025_026 Indel 258799855 321 (T); 326 (M) (F) GTACCTCCGACGACTCGAAA (R) CATAGAGCTGGTGGGGAGTG

GBS_121 GBS 258800041 SNP -

GBS_122 GBS 258800198 SNP -

GBS_123 GBS 258800389 SNP -

GBS_124 GBS 258803723 SNP -

GBS_125 GBS 258803752 SNP -

GBS_126 GBS 258808508 SNP -

GBS_127 GBS 258875029 SNP -

GBS_128 GBS 258878686 SNP -

GBS_129 GBS 258878734 SNP -

LEK037_038 (SBM01)

SBM 259017755 SNP + Indel (F) GGCCTAGACACTGCCTTCTG (R) TTGGCTGAATCCTTCTCGTT

GBS_130 GBS 259017915 SNP -

GBS_131 GBS 259023565 SNP -

GBS_132 GBS 259023731 SNP -

GBS_133 GBS 259024225 SNP -

GBS_134 GBS 259174085 SNP -

GBS_135 GBS 259174254 SNP -

GBS_136 GBS 259175533 SNP -

GBS_137 GBS 259175601 SNP -

GBS_138 GBS 259175680 SNP -

GBS_139 GBS 259180657 SNP -

GBS_140 GBS 259181338 SNP -

GBS_141 GBS 259210821 SNP -

GBS_142 GBS 259212357 SNP -

GBS_143 GBS 259212464 SNP -

GBS_144 GBS 259214758 SNP -

GBS_145 GBS 259215322 SNP -

GBS_146 GBS 259215767 SNP -

GBS_147 GBS 259217246 SNP -

GBS_148 GBS 259217414 SNP -

GBS_149 GBS 259217670 SNP -

GBS_150 GBS 259217673 SNP -

GBS_151 GBS 259219693 SNP -

GBS_152 GBS 259219993 SNP -

GBS_153 GBS 259220072 SNP -

GBS_154 GBS 259220966 SNP -

GBS_155 GBS 259220974 SNP -

GBS_156 GBS 259221159 SNP -

GBS_157 GBS 259221612 SNP -

GBS_158 GBS 259221873 SNP -

GBS_159 GBS 259222739 SNP -

GBS_160 GBS 259223303 SNP -

GBS_161 GBS 259223380 SNP -

GBS_162 GBS 259223619 SNP -

GBS_163 GBS 259225324 SNP -

GBS_164 GBS 259225871 SNP -

GBS_165 GBS 259235250 SNP -

GBS_166 GBS 259235364 SNP -

GBS_167 GBS 259239221 SNP -

GBS_168 GBS 259272819 SNP -

GBS_169 GBS 259272837 SNP -

GBS_170 GBS 259274478 SNP -

GBS_171 GBS 259301362 SNP -

GBS_172 GBS 259301727 SNP -

GBS_173 GBS 259304663 SNP -

GBS_174 GBS 259304819 SNP -

umc1298 SSR 259304887 177 (T); 179 (M) (F) AGGACAAGAAAAAGAAGAAGCACG (R) AGCTGAACAAAATAAACGGAACGA

GBS_175 GBS 259304921 SNP -

GBS_176 GBS 259304941 SNP -

GBS_177 GBS 259372795 SNP -

GBS_178 GBS 259379916 SNP -

GBS_179 GBS 259407119 SNP -

GBS_180 GBS 259407231 SNP -

GBS_181 GBS 259408089 SNP -

GBS_182 GBS 259532659 SNP -

GBS_183 GBS 259538298 SNP -

GBS_184 GBS 259701285 SNP -

LEK055_056 (SBM02)

SBM 259714872 SNP + Indel (F) GGCATAGCTCGAGGAACAAC (R) TGTAGAAGGGGATTTGGCTG

GBS_185 GBS 259746774 SNP -

GBS_186 GBS 259771415 SNP -

GBS_187 GBS 259772662 SNP -

GBS_188 GBS 259772740 SNP -

GBS_189 GBS 259778419 SNP -

GBS_190 GBS 259816063 SNP -

GBS_191 GBS 259817618 SNP -

GBS_192 GBS 259825061 SNP -

GBS_193 GBS 259930666 SNP -

GBS_194 GBS 259937100 SNP -

GBS_195 GBS 259937306 SNP -

GBS_196 GBS 259937627 SNP -

GBS_197 GBS 259948037 SNP -

GBS_198 GBS 259948106 SNP -

GBS_199 GBS 259949194 SNP -

GBS_200 GBS 259949290 SNP -

GBS_201 GBS 260142416 SNP -

GBS_202 GBS 260143885 SNP -

GBS_203 GBS 260144220 SNP -

GBS_204 GBS 260144338 SNP -

GBS_205 GBS 260150212 SNP -

GBS_206 GBS 260150230 SNP -

GBS_207 GBS 260150257 SNP -

GBS_208 GBS 260150325 SNP -

GBS_209 GBS 260338377 SNP -

GBS_210 GBS 260338525 SNP -

GBS_211 GBS 260339199 SNP -

LEK067_068 Indel 260366895 130 (M); 138 (T) (F) GCATCTTGTTCAAATTAGCAGC (R) GTGAGTACTGTTACTAGCTTGTTATGC

CJ071_021 (SBM03)

SBM 260383398 SNP + Indel (F) TCTGGTTATGCGAAAGAAGC (R) TCTGCTGGTTGATGGACTAC

CJ136_118 (SBM04)

SBM 260448142 SNP + Indel (F) TGTCTTGAGWGGTTCTAGTG (R) AGTTCATTCACCTGTGTTGG

CJ004_005 (SBM05)

SBM 260448854 SNP + Indel (F) GTTCATTCTATTTCCAGGAC (R) CCAGGCACCTGATGGAATC

GBS_212 GBS 260514553 SNP -

GBS_213 GBS 260514645 SNP -

GBS_214 GBS 260517297 SNP -

GBS_215 GBS 260517356 SNP -

GBS_216 GBS 260522263 SNP -

GBS_217 GBS 260621279 SNP -

GBS_218 GBS 260655699 SNP -

GBS_219 GBS 260655766 SNP -

GBS_220 GBS 260655851 SNP -

LEK092_093 Indel 260656326 211 (T); 217 (M) (F) CTGATGCGTAGCGTATATCGAG (R) AGGCCTCCTTTGATCCCAA

LEK095_096 (SBM06)

SBM 260679274 SNP + Indel (F) TTGCTGATATTGTTCGCTCG (R) CAACTCGATGGAAAGCCATT

LEK097_098 (SBM07)

SBM 260684606 SNP + Indel (F) ACATCATCCCCGTGAAAGAG (R) TCAAGTGGTTCTCCCAGTCC

GBS_221 GBS 260711087 SNP -

GBS_222 GBS 260745399 SNP -

GBS_223 GBS 260746476 SNP -

GBS_224 GBS 260771404 SNP -

umc1411 SSR - Small (T); Large (M) (F) CGATAATGAAGATGAGCTCCGTG (R) CTAGCCGGTTGTTGTTGCCT

Table S2. Primers used for sequencing ZmYAB2.1

Primer Sequence (5’ – 3’) Location Direction CJ021 TCTGCTGGTTGATGGACTAC 5’ UTR Forward CJ074 ATGTCGTCGGCGGCCCAGAT Exon 1 Forward CJ022 AGACGAAGCTAGTCAGCAAG Intron 1 Forward CJ115 GTTCTCAAAGCATTCTCGCC Intron 1 Forward CJ071 TCTGGTTATGCGAAAGAAGC Intron 1 Reverse CJ072 TCGATCAAGAAGGTAACGAG Intron 1 Reverse CJ024 CCAGGTCATCGGCAGTACAC Intron 1 Reverse CJ025 CCACAAACCTTAGTGACAATG Intron 1 Forward CJ026 CCGCTATGCTAAAACATTATTC Intron 1 Forward CJ116 GCACAAGAGAACAACGATAG Intron 1 Reverse CJ114 AACCTCATGTATTGTCTCGC Intron 1 Forward CJ027 GGACTAATAAGAGCTGGACG Intron 1 Reverse CJ028 AAGCATATCAGTCCTGGTTTG Intron 1 Reverse CJ096 TTCGAATGACTTGTAGTTCAG Intron 1 Forward CJ131 CCATATACTCTCACAACAGC Intron 1 Forward CJ135 ACCTCCACCTCAGGTTATTC Intron 1 Forward CJ168 CGCACTGTGTTGTAATGAAC Intron 1 Forward CJ169 AAAGCCTTATTTTCGGCCAG Intron 1 Forward CJ133 CGCCGAAAATAAGCCAGTTC Intron 1 Reverse CJ130 TTGTTGACACTCGGCAAAGT Intron 1 Reverse CJ132 AGTGCCTTTGTTGACACTCG Intron 1 Reverse CJ129 TAACAATCGTGAGCTGTCGT Intron 1 Forward CJ127 ACACTCGACAAAAAGGTGGT Intron 1 Reverse CJ103 GCGTYTCGGATAGTGGAAGG Intron 1 Forward CJ109 AAACSACCTTCCACTATCCG Intron 1 Reverse CJ104 GTTGCRTGAAACTAAATGGC Intron 1 Forward CJ126 TTAATGTGGAGAACGGAACA Intron 1 Reverse CJ125 TACTGCCACTTCGTTCAGAG Intron 1 Reverse CJ120 GTTTACTGGTCGATCTTTGC Intron 1 Forward CJ107 TGGAGCACGTACACACAATC Intron 1 Forward CJ106 TGATTGTGTGTACGTGCTCC Intron 1 Reverse CJ196 AGATCAACCGTCGTGTAGTG Intron 1 Reverse CJ198 CCACCATCAGTCACACACTT Intron 1 Forward CJ105 TTTGATTCCTCACGCTGTCC Intron 1 Reverse CJ101 TTTGCAGAAGAACGAGGAGT Intron 1 Reverse CJ057 AACATGGTGACAGTCCGTTG Exon 2 Forward CJ058 GTGAGTAGTGATTCTGGACG Exon 2 Reverse CJ085 TCAAGGTCCAAAATTTCAGC Exon 3 Forward CJ055 ACGTGCAGCATATGATCCAG Exon 3 Reverse CJ054 GTTATATGCTGAAGGAACACG Exon 4 Reverse CJ075 CTTATGTCCGGGTTGTTTGC Exon 5 Reverse CJ061 GTTGCACCAATGGTCTCATC Exon 6 Reverse CJ079 TCATTCGTGATACTAGGAGC 3’ UTR Reverse

Table S3. Samples used for DNA sequence analysis and selection tests

Line DNA Prep Type Race/Species Country MR01 BKN_023 Landrace Inbred Araguito Venezuela MR02 BKN_014 Landrace Inbred Assiniboine USA MR05 BKN_019 Landrace Inbred Cateto Bolivia MR06 BKN_033 Landrace Inbred Chapalote Mexico MR08 BKN_034 Landrace Inbred Costeno Venezuela MR09 BKN_032 Landrace Inbred Cravo Riogranense Brazil MR12 BKN_015 Landrace Inbred Havasupai USA MR13 BKN_040.1 Landrace Inbred Hickory King USA MR14 BKN_016 Landrace Inbred Longfellow Flint Canada MR15 BKN_028 Landrace Inbred Palomero De Jalisco Mexico MR17 BKN_026 Landrace Inbred Pisankalla Bolivia MR18 BKN_022 Landrace Inbred Reventador Mexico MR19 BKN_017 Landrace Inbred Santa Domingo USA MR20 BKN_018 Landrace Inbred Shoe Peg USA MR21 BKN_035 Landrace Inbred Tabloncillo Mexico MR22 BKN_025 Landrace Inbred Tuxpeno Mexico MR23 BKN_030 Landrace Inbred Zapalote Chico Mexico MR24 BKN_009 Landrace Inbred Chullpi Peru MR25 BKN_010 Landrace Inbred Pororo Bolivia TIL01 TIP-521.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL03 TIP-523.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL04 TIP-454.5 Teosinte Inbred Zea mays ssp. parviglumis - TIL05 TIP-458.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL06 TIP-496.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL07 TIP-466.5 Teosinte Inbred Zea mays ssp. parviglumis - TIL08 TIP-529.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL09 TIP-503.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL10 TIP-485.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL11 TIP-534.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL12 TIP-508.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL14 TIP-498.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL16 TIP-469.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL17 TIP-512.1 Teosinte Inbred Zea mays ssp. parviglumis - TIL18 TIP-475.5 Teosinte Inbred Zea mays ssp. mexicana - TIL25 TIP-489.1 Teosinte Inbred Zea mays ssp. mexicana - M006 Q171 Open pollinated Zea diploperennis -

Table S4. Samples used for surveying allele distribution

Accession Stock Subspecies Type Racename Country Stateprovince Population Latitude Longitude Altitude

(ft) W22 Spm

B73 Spm

Mo17 82bp

Ki3 2bp

N/A 38-11 (PURD-AG) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A A158 (MINN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A A441-5 (NATAL) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A A6 (PHI) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A A619 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A B37 (ISU) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A B73 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A B97 (ISU) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CM105 (AGCAN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CM174 (AGCAN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A CM37 (BB) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A CML103 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A CML228 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CML247 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A CML277 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A CML322 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A CML333 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CML52 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A CML69 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A H99 (PURD-AG) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A Hp301 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A I29 (PURD-BOT) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Ia2132 (UIUC-NR) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Il14H (UIUC-NR) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K13 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K133 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K141 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K187 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K39 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K42 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K50 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A K55 (ARS-MO) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A Ki11 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A Ki3 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- +/+

N/A Ky21 (KY) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A M162W mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A M37W mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A Mo17 (ARS-MO) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A MO18W mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A MS153 (MICH) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A MS71 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A N192 (NEBR) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A NC350 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A NC358 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A Oh43 (OHW) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Oh7B (OHW) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A P39 (PURD-AG) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Q229 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A SC55 (SC) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A Sg18 (PURD-BOT) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A T232 (BB) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A T8 (TENN) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+ -/-

N/A Tx303 mays Inbred N/A N/A N/A N/A N/A N/A N/A N/A -/- -/- +/+

N/A Tzi8 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A Va26 (VATECH) mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- +/+ -/- -/-

N/A W182B (WISC) mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A W22 mays Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- -/-

N/A Z134 mays Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL01-S8 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL02-S3 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL03-S8 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL04-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL05-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL06-S6 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL07-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL08-S8 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL09-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A +/+ -/- -/- +/+

N/A TIL10-S6 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL11-S9 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL12-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL14-S6 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL15-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL16-S5 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL17-S7 parviglumis Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL18-S4 mexicana Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

N/A TIL25-S5 mexicana Inbred N/A N/A N/A N/A N/A N/A N/A -/- -/- -/- -/-

8750 8750 (CIMMYT) mexicana OP Chalco Mexico Mexico Ozumba 19.05 N 98.82 W 2340 -/- -/- -/- -/-

8769 8769 (CIMMYT) mexicana OP Chalco Mexico Distrito Federal

San Mateo 19.23 N 99.12 W 2300 -/- -/- -/- -/-

8770 8770 (CIMMYT) mexicana OP Chalco Mexico Mexico Zoquiapan 19.32 N 98.85 W 2270 -/- -/- -/- -/-

11386 11386 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.27 N 98.92 W 2200 -/- -/- -/- -/-

11386 11386 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.27 N 98.92 W 2200 -/- -/- -/- -/-

11374 11374 (CIMMYT) mexicana OP Chalco Mexico Mexico Ixtapaluca 19.35 N 98.88 W 2200 -/- -/- -/- -/-

11364 11364 (CIMMYT) mexicana OP Chalco Mexico Mexico Tlalmanalco 19.20 N 98.80 W 2400 -/- -/- -/- -/-

11362 11362 (CIMMYT) mexicana OP Chalco Mexico Distrito Federal

Xochimilco 19.25 N 99.08 W 2200 -/- -/- -/- -/-

11371 11371 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Puruandiro 20.08 N 101.52 W 2000 -/- -/- -/- -/-

11367 11367 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Chucandiro 19.90 N 101.33 W 1800 -/- -/- -/- -/-

11370 11370 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Puruandiro 20.08 N 101.52 W 2000 -/- -/- -/- -/-

11372 11372 (CIMMYT) mexicana OP Central Plateau

Mexico Guanajuato Moroleon 20.08 N 101.22 W 2100 -/- -/- -/- -/-

11373 11373 (CIMMYT) mexicana OP Central Plateau

Mexico Guanajuato Pinicuaro 20.05 N 101.23 W 2150 -/- -/- -/- -/-

12823 12823 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.25 N 98.85 W 2130 -/- -/- -/- -/-

12823 12823 (CIMMYT) mexicana OP Chalco Mexico Mexico Chalco 19.25 N 98.85 W 2130 -/- -/- -/- -/-

11408 11408 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Villa

Escalante 19.40 N 101.60 W 2320 -/- -/- -/- -/-

12825 12825 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Copandaro 19.88 N 101.22 W 1650 -/- -/- -/- -/-

11409 11409 (CIMMYT) mexicana OP Chalco Mexico Mexico Texcoco 19.50 N 98.92 W 2200 -/- -/- -/- -/-

11409 11409 (CIMMYT) mexicana OP Chalco Mexico Mexico Texcoco 19.50 N 98.92 W 2200 -/- -/- -/- -/-

11399 11399 (CIMMYT) mexicana OP Chalco Mexico Mexico Amecameca 19.13 N 98.77 W 2390 -/- -/- -/- -/-

11392 11392 (CIMMYT) mexicana OP Central Plateau

Mexico Durango Puente

Gavilanes 24.02 N 104.48 W 1950 -/- -/- -/- -/-

11394 11394 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Degollado 20.10 N 101.90 W 1700 -/- -/- -/- -/-

13792 13792 (CIMMYT) mexicana OP Chalco Mexico Mexico El Pedregal 19.27 N 99.45 W 2500 -/- -/- -/- -/-

13788 13788 (CIMMYT) mexicana OP Chalco Mexico Puebla Santa Cruz 19.08 N 97.53 W 2425 -/- -/- -/- -/-

13791 13791 (CIMMYT) mexicana OP Chalco Mexico Puebla Tlachichuca 19.17 N 97.60 W 2355 -/- -/- -/- -/-

5630 5630 (HHI) mexicana OP Central Plateau

Mexico Jalisco La Estancia 21.50 N 101.85 W 1920 -/- +/+ -/- -/-

13790 13790 (CIMMYT) mexicana OP Chalco Mexico Puebla San Salvador 19.13 N 97.63 W 2425 -/- -/- -/- -/-

13789 13789 (CIMMYT) mexicana OP Chalco Mexico Puebla San Antonio 19.07 N 97.53 W 2440 -/- -/- -/- -/-

13793 13793 (CIMMYT) mexicana OP Chalco Mexico Mexico Chapultepec 19.20 N 99.55 W 2540 -/- -/- -/- -/-

PI566673 PI566673 (NCRPIS) mexicana OP Central Plateau

Mexico Durango Durango 24.18 N 104.40 W 1950 -/- -/- -/- -/-

8750 8750 (CIMMYT) mexicana OP Chalco Mexico Mexico Ozumba 19.05 N 98.82 W 2340 -/- -/- -/- -/-

8751 8751 (CIMMYT) mexicana OP Chalco Mexico Mexico Temamatla 19.18 N 98.88 W 2400 -/- -/- -/- -/-

8770 8770 (CIMMYT) mexicana OP Chalco Mexico Mexico Zoquiapan 19.32 N 98.85 W 2270 -/- -/- -/- -/-

11362 11362 (CIMMYT) mexicana OP Chalco Mexico Distrito Federal

Xochimilco 19.25 N 99.08 W 2200 -/- -/- -/- -/-

11372 11372 (CIMMYT) mexicana OP Central Plateau

Mexico Guanajuato Moroleon 20.08 N 101.22 W 2100 -/- -/- -/- -/-

11373 11373 (CIMMYT) mexicana OP Central Plateau

Mexico Guanajuato Pinicuaro 20.05 N 101.23 W 2150 -/- -/- -/- -/-

11374 11374 (CIMMYT) mexicana OP Chalco Mexico Mexico Ixtapaluca 19.35 N 98.88 W 2200 -/- -/- -/- -/-

11387 11387 (CIMMYT) mexicana OP Nobogame Mexico Chihuahua Guadalupe y

Calvo 26.23 N 106.97 W 1850 -/- -/- -/- -/-

11392 11392 (CIMMYT) mexicana OP Central Plateau

Mexico Durango Puente

Gavilanes 24.02 N 104.48 W 1950 -/- -/- -/- -/-

11394 11394 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Degollado 20.10 N 101.90 W 1700 -/- -/- -/- -/-

11399 11399 (CIMMYT) mexicana OP Chalco Mexico Mexico Amecameca 19.13 N 98.77 W 2390 -/- -/- -/- -/-

11400 11400 (CIMMYT) mexicana OP Chalco Mexico Mexico Los Reyes

La Paz 19.40 N 98.95 W 2200 -/- -/- -/- -/-

11409 11409 (CIMMYT) mexicana OP Chalco Mexico Mexico Texcoco 19.50 N 98.92 W 2200 -/- -/- -/- -/-

12825 12825 (CIMMYT) mexicana OP Central Plateau

Mexico Michoacan Copandaro 19.88 N 101.22 W 1650 -/- -/- -/- -/-

13788 13788 (CIMMYT) mexicana OP Chalco Mexico Puebla Santa Cruz 19.08 N 97.53 W 2425 -/- -/- -/- -/-

13789 13789 (CIMMYT) mexicana OP Chalco Mexico Puebla San Antonio 19.07 N 97.53 W 2440 -/- -/- -/- -/-

13790 13790 (CIMMYT) mexicana OP Chalco Mexico Puebla San Salvador 19.13 N 97.63 W 2425 -/- -/- -/- -/-

13791 13791 (CIMMYT) mexicana OP Chalco Mexico Puebla Tlachichuca 19.17 N 97.60 W 2355 -/- -/- -/- -/-

13792 13792 (CIMMYT) mexicana OP Chalco Mexico Mexico El Pedregal 19.27 N 99.45 W 2500 -/- -/- -/- -/-

13793 13793 (CIMMYT) mexicana OP Chalco Mexico Mexico Chapultepec 19.20 N 99.55 W 2540 -/- -/- -/- -/-

11066 11066 (LMP) mexicana OP Central Plateau

Mexico Jalisco 10 km S of Degollado

20.37 N 102.18 W 1625 -/- -/- -/- -/-

JSG Y LOS-3

JSG Y LOS -3 mexicana OP Central Plateau

Mexico Jalisco Ayotlan 20.42 N 102.35 W 1520 -/- -/- -/- -/-

8779 T. A. Kato K-71-7 parviglumis OP Balsas Mexico Mexico Valle De

Bravo 19.17 N 100.32 W 1000 -/- -/- -/- -/-

JSG-187 J. Sanchez JSG-187 parviglumis OP Balsas Mexico Michoacan Huetamo 18.82 N 100.97 W 740 -/- -/- -/- -/-

JSG Y LOS-113

Sanchez & Ordaz JSG Y LOS -113

parviglumis OP Balsas Mexico Guerrero Teloloapan 18.32 N 99.80 W 1500 -/- -/- -/- -/-

JSG Y SMH-355

Sanchez & Montes JSG Y SMH -355

parviglumis OP Jalisco Mexico Jalisco Ejutla 19.95 N 104.00 W 950 -/- -/- -/- -/-

8759 T. A. Kato K-67-12 parviglumis OP Balsas Mexico Guerrero Acapetlahua

ya 18.33 N 100.12 W 970 -/- -/- -/- -/-

PI566688 Wilkes & Sanchez

WS-92-12 parviglumis OP Balsas Mexico Guerrero Zacatlan 18.38 N 99.97 W 1720 -/- -/- -/- -/-

JSG Y LOS-172

Sanchez & Ordaz JSG Y LOS -172

parviglumis OP Balsas Mexico Mexico Valle De

Bravo 19.17 N 100.20 W 1620 -/- -/- -/- -/-

8782 H. G. Wilkes W-71-3 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.35 N 100.18 W 1050 -/- -/- -/- -/-

JSG-377 J. Sanchez JSG-377 parviglumis OP Balsas Mexico Guerrero Tecoanapa 17.00 N 99.28 W 625 -/- -/- -/- -/-

C-9-78 Cervantes C-9-78 parviglumis OP Balsas Mexico Guerrero Ixcateopan 18.33 N 99.80 W 1640 -/- -/- -/- -/-

11376 T. A. Kato K-69-14 parviglumis OP Balsas Mexico Guerrero El Rincon 17.28 N 99.50 W 1150 -/- -/- -/- -/-

11401 Wilkes & Sanchez

WS-92-10 parviglumis OP Balsas Mexico Guerrero Huitzuco 18.28 N 99.18 W 1225 -/- -/- -/- -/-

JSG Y LOS-178

Sanchez & Ordaz JSG Y LOS -178

parviglumis OP Balsas Mexico Mexico Otzoloapan 19.17 N 100.32 W 1320 -/- -/- -/- -/-

JSG-391 J. Sanchez JSG-391 parviglumis OP Balsas Mexico Mexico Zacazonapan 19.08 N 100.27 W 1420 -/- -/- -/- -/-

JSG Y MAS-400

Sanchez & Aguilar JSG Y MAS-400

parviglumis OP Jalisco Mexico Jalisco Jirosto 19.77 N 104.75 W 480 -/- -/- -/- -/-

8766 T. A. Kato K-67-23 parviglumis OP Balsas Mexico Michoacan Tuzantla 19.18 N 100.55 W 1040 -/- -/- -/- -/-

JSG Y SMH-352

Sanchez & Montes JSG Y SMH -352

parviglumis OP Jalisco Mexico Jalisco Ejutla 19.92 N 104.15 W 1140 -/- -/- -/- -/-

IC #3 Iltis & Cochrane Site 3 parviglumis OP Balsas Mexico Guerrero site 2,

Teloloapan Hwy

18.33 N 99.83 W 1600 -/- -/- -/- -/-

PI566691 Wilkes & Sanchez

WS-92-? parviglumis OP Balsas Mexico Michoacan Tzitzio 19.45 N 100.90 W 1100 -/- -/- -/- -/-

JSG Y LOS-130

Sanchez & Ordaz JSG Y LOS -130

parviglumis OP Balsas Mexico Michoacan Tzitzio 19.60 N 100.92 W 1500 -/- -/- -/- -/-

JSG-379 J. Sanchez JSG-379 parviglumis OP Balsas Mexico Guerrero Mochitlan 17.28 N 99.48 W 765 -/- -/- -/- -/-

8765 T. A. Kato K-67-22 parviglumis OP Balsas Mexico Michoacan Tuzantla 19.08 N 100.47 W 950 -/- -/- -/- -/-

C-17-78 Cervantes C-17-78 parviglumis OP Balsas Mexico Michoacan Huetamo 18.83 N 100.93 W 710 -/- -/- -/- -/-

JSG Y LOS-74

Sanchez & Ordaz JSG Y LOS -74

parviglumis OP Jalisco Mexico Jalisco Villa

Purificacion 19.70 N 104.82 W 520 -/- -/- -/- -/-

C-14-78 Cervantes C-14-78 parviglumis OP Balsas Mexico Mexico Luvianos 18.98 N 100.32 W 1200 -/- -/- -/- -/-

PI384064 H. G. Wilkes Wilkes 1 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.35 N 99.85 W 1550 -/- -/- -/- -/-

8760 T. A. Kato K-67-14 parviglumis OP Balsas Mexico Michoacan Tiquiche 18.85 N 100.90 W 720 -/- -/- -/- -/-

JSG-378 J. Sanchez JSG-378 parviglumis OP Balsas Mexico Guerrero Tierra

Colorada 17.18 N 99.52 W 420 -/- -/- -/- -/-

JSG Y MAS-401

Sanchez & Aguilar JSG Y MAS-401

parviglumis OP Jalisco Mexico Jalisco Toliman 19.53 N 104.07 W 1405 -/- -/- -/- -/-

JSG Y LOS-126

Sanchez & Ordaz JSG Y LOS -126

parviglumis OP Balsas Mexico Michoacan Benito Juarez

19.28 N 100.42 W 1100 -/- -/- -/- -/-

8763 T. A. Kato K-67-20 parviglumis OP Balsas Mexico Mexico Tingambato 19.22 N 100.28 W 1110 -/- -/- -/- -/-

8761 T. A. Kato K-67-15 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.37 N 100.87 W 1120 -/- -/- -/- -/-

BK Site 4 Beadle & Kato Site 4 parviglumis OP Balsas Mexico Guerrero 1 mile S of

Palo Blanco 17.42 N 99.50 W 1350 -/- -/- -/- -/-

11407 Wilkes & Sanchez

WS-92-16 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.60 N 100.92 W 1500 -/- -/- -/- -/-

11406 Wilkes & Sanchez

WS-92-15 parviglumis OP Balsas Mexico Michoacan

Benito Juarez

19.22 N 100.52 W 880 -/- -/- -/- -/-

11353 T. A. Kato K-67-8 parviglumis OP Balsas Mexico Guerrero Iguala 18.35 N 99.78 W 1570 -/- -/- -/- -/-

JSG Y LOS-121

Sanchez & Ordaz JSG Y LOS -121

parviglumis OP Balsas Mexico Guerrero Acapetlahua

ya 18.38 N 100.07 W 1150 -/- -/- -/- -/-

8784 G. Beadle B-72-1 parviglumis OP Balsas Mexico Guerrero El Salado 17.40 N 99.40 W 1300 -/- -/- -/- -/-

8758 T. A. Kato K-67-7 parviglumis OP Balsas Mexico Guerrero Ixcateopan 18.37 N 99.77 W 1560 -/- -/- -/- -/-

JSG Y LOS-109

Sanchez & Ordaz JSG Y LOS -109

parviglumis OP Balsas Mexico Guerrero Chilpancingo 17.40 N 99.47 W 1260 -/- -/- -/- -/-

JSG-203 J. Sanchez JSG-203 parviglumis OP Jalisco Mexico Jalisco Juchitlan 19.98 N 104.05 W 1000 -/- -/- -/- -/-

967 B. Benz 967 parviglumis OP Jalisco Mexico Jalisco El Rodeo, below La

Lima 19.55 N 104.05 W 1460 -/- -/- -/- -/-

JSG-387 J. Sanchez JSG-387 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.35 N 99.83 W 1560 -/- -/- -/- -/-

JSG-193 J. Sanchez JSG-193 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.45 N 100.90 W 1100 -/- -/- -/- -/-

11357 T. A. Kato K-67-13 parviglumis OP Balsas Mexico Michoacan Huetamo 18.82 N 100.97 W 740 -/- -/- -/- -/-

8762 T. A. Kato K-67-17 parviglumis OP Balsas Mexico Mexico Luvianos 18.90 N 100.15 W 1390 -/- -/- -/- -/-

8783 H. G. Wilkes W-71-4 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.32 N 99.80 W 1580 -/- -/- -/- -/-

JSG-192 J. Sanchez JSG-192 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.37 N 100.87 W 1120 -/- -/- -/- -/-

JSG-374 J. Sanchez JSG-374 parviglumis OP Balsas Mexico Morelos Tepoztlan 18.97 N 99.03 W 1580 -/- -/- -/- -/-

JSG Y LOS-159

Sanchez & Ordaz JSG Y LOS -159

parviglumis OP Balsas Mexico Mexico Malinalco 18.95 N 99.50 W 1850 -/- -/- -/- -/-

11404 Wilkes & Sanchez

WS-92-13 parviglumis OP Balsas Mexico Mexico Amatepec 18.65 N 100.40 W 1180 -/- -/- -/- -/-

JSG Y MAS-264

Sanchez & Aguilar JSG Y MAS -264

parviglumis OP Jalisco Mexico Nayarit Amatlan De

Canas 20.80 N 104.40 W 900 -/- -/- -/- -/-

JSG-385 J. Sanchez JSG-385 parviglumis OP Balsas Mexico Guerrero Ixcateopan 18.50 N 99.78 W 1795 -/- -/- -/- -/-

JSG Y MAS-402

Sanchez & Aguilar JSG Y MAS-402

parviglumis OP Jalisco Mexico Jalisco Guachinango 20.63 N 104.42 W 1385 -/- -/- -/- -/-

JSG Y LOS-43

Sanchez & Ordaz JSG Y LOS -43

parviglumis OP Jalisco Mexico Jalisco Guachinango 20.80 N 104.55 W 1090 -/- -/- -/- -/-

JSG Y LOS-161

Sanchez & Ordaz JSG Y LOS -161

parviglumis OP Balsas Mexico Mexico Tejupilco 18.90 N 100.20 W 1320 -/- -/- -/- -/-

MAS-15 M. Aguilar MAS-15 parviglumis OP Jalisco Mexico Colima Minatitlan 19.45 N 104.03 W 1100 -/- -/- -/- -/-

11403 Wilkes & Sanchez

WS-92-12 parviglumis OP Balsas Mexico Guerrero Zacatlan 18.38 N 99.97 W 1720 -/- -/- -/- -/-

8776 T. A. Kato K-71-4 parviglumis OP Balsas Mexico Guerrero Palo Blanco 17.40 N 99.47 W 900 -/- -/- -/- -/-

11361 T. A. Kato K-67-25 parviglumis OP Balsas Mexico Michoacan Benito Juarez

19.27 N 100.47 W 1235 -/- -/- -/- -/-

JSG-197 J. Sanchez JSG-197 parviglumis OP Balsas Mexico Oaxaca S. Pedro

Juchatengo 16.33 N 97.03 W 1120 -/- -/- -/- -/-

JSG Y LOS-120

Sanchez & Ordaz JSG Y LOS -120

parviglumis OP Balsas Mexico Guerrero Acapetlahua

ya 18.37 N 100.03 W 1500 -/- -/- -/- -/-

JSG-191 J. Sanchez JSG-191 parviglumis OP Balsas Mexico Michoacan Tzitzio 19.38 N 100.82 W 1000 -/- -/- -/- -/-

PI566686 Wilkes & Sanchez

WS-92-10 parviglumis OP Balsas Mexico Guerrero Huitzuco 18.28 N 99.18 W 1225 -/- -/- -/- -/-

JSG Y LOS-142

Sanchez & Ordaz JSG Y LOS -142

parviglumis OP Jalisco Mexico Jalisco Jilotlan 19.30 N 103.08 W 1460 -/- -/- -/- -/-

11355 T. A. Kato K-67-10 parviglumis OP Balsas Mexico Guerrero Teloloapan 18.40 N 99.90 W 1800 -/- -/- -/- -/-

JSG Y LOS-119

Sanchez & Ordaz JSG Y LOS -119

parviglumis OP Balsas Mexico Guerrero Ixcapuzalco 18.40 N 99.97 W 1620 -/- -/- -/- -/-

8767 T. A. Kato K-67-24 parviglumis OP Balsas Mexico Michoacan Tuzantla 19.22 N 100.52 W 880 -/- -/- -/- -/-

JSG Y LOS-176

Sanchez & Ordaz JSG Y LOS -176

parviglumis OP Balsas Mexico Mexico Otzoloapan 19.15 N 100.38 W 1260 -/- -/- -/- -/-

JSG Y LOS-40

Sanchez & Ordaz JSG Y LOS -40

parviglumis OP Jalisco Mexico Jalisco Mascota 20.80 N 104.58 W 620 -/- -/- -/- -/-

JSG-382 J. Sanchez JSG-382 parviglumis OP Balsas Mexico Guerrero Chilpancingo 17.43 N 99.48 W 1300 -/- -/- -/- -/-

9477 M. Puga TEO:

BALSAS parviglumis OP Jalisco Mexico Jalisco

Cerro La Mesa

19.82 N 104.18 W 850 -/- -/- -/- -/-

11388 Wilkes, Sanchez &

Taba WST-85-3 parviglumis OP Balsas Mexico Oaxaca Oaxaca 16.33 N 97.03 W 1120 -/- -/- -/- -/-

AYA 32 MR24 mays Inbred Chullpi Peru N/A N/A 12.93 S 74.25 W 2350 -/- -/- -/- -/-

CUN 465 MR26 mays Inbred Pollo Colombia N/A N/A 5.05 N 73.50 W 1600 +/- +/- -/- -/-

URG II MR28 mays Inbred Cateto Sulino

Uruguay N/A N/A 31.70 S 56.00 W N/A -/- -/- -/- -/-

PI213793 MR02 mays Inbred Assiniboine USA N/A N/A 46.82 N 100.82 W 457 -/- -/- +/- -/-

PI317675 MR12 mays Inbred Havasupai USA N/A N/A 36.25 N 112.67 W 1067 -/- -/- -/- -/-

PI217408 MR14 mays Inbred Longfellow

Flint Canada N/A N/A 42.25 N 71.50 W N/A -/- -/- +/+ -/-

PI218130 MR19 mays Inbred Santa

Domingo USA N/A N/A 35.52 N 106.35 W 1585 -/- -/- -/- -/-

PI269743 MR20 mays Inbred Shoe Peg USA N/A N/A 36.78 N 92.22 W 213 -/- -/- +/+ -/-

BOV 635 MR05 mays Inbred Cateto Bolivia N/A N/A 16.60 S 67.25 W 1801 -/- +/+ -/- -/-

CUB 65 MR11 mays Inbred Cuban Flint Cuba N/A N/A 23.08 N 82.67 W 183 +/+ -/- -/- -/-

MOR 17 MR16 mays Inbred Pepetilla Mexico N/A N/A 18.35 N 99.53 W 747 -/- -/- -/- -/-

NAY 15 MR18 mays Inbred Reventador Mexico N/A N/A 22.33 N 105.38 W 46 -/- -/- -/- -/-

VEN 568 MR01 mays Inbred Araguito Venezuela N/A N/A 8.90 N 64.22 W 183 -/- -/- -/- -/-

VEN 604 MR04 mays Inbred Canilla Venezuela N/A N/A 8.90 N 64.22 W 183 -/- -/- -/- -/-

TAM 125 MR22 mays Inbred Tuxpeno Mexico N/A N/A 22.28 N 97.88 W 150 -/- -/- -/- -/-

BOV 344 MR17 mays Inbred Pisankalla Bolivia N/A N/A 21.52 S 64.75 W 1738 -/- -/- -/- -/-

CHI 349 MR10 mays Inbred Cristalino

Norteno Chile N/A N/A 39.92 S 72.50 W 7 -/- -/- -/- -/-

JAL 142 MR15 mays Inbred Palomero De

Jalisco Mexico N/A N/A 20.03 N 103.68 W 2520 -/- -/- -/- -/-

OAX 68 MR03 mays Inbred Bolita Mexico N/A N/A 17.47 N 97.30 W 1646 +/- -/- -/- -/-

OAX 70 MR23 mays Inbred Zapalote

Chico Mexico N/A N/A 16.33 N 95.23 W 46 -/- -/- -/- -/-

CHS 86 MR07 mays Inbred Comiteco Mexico N/A N/A 16.25 N 92.13 W 1555 +/+ -/- -/- -/-

RGS VII MR09 mays Inbred Cravo

Riogranense Brazil N/A N/A 30.00 S 53.00 W 366 -/- +/+ -/- -/-

SIN 2 MR06 mays Inbred Chapalote Mexico N/A N/A 24.80 N 107.42 W 61 -/- -/- -/- -/-

VEN 453 MR08 mays Inbred Costeno Venezuela N/A N/A 9.02 N 63.72 W 146 -/- +/+ -/- -/-

JAL 43 MR21 mays Inbred Tabloncillo Mexico N/A N/A 19.98 N 104.27 W 1330 -/- -/- -/- -/-

PI311237 MR13 mays Inbred Hickory

King USA N/A N/A 37.22 N 80.42 W N/A -/- -/- +/+ -/-

GTO 73 GTO 73 (INIFAP) mays OP Conico

Norteno Mexico N/A N/A 20.37 N 101.07 W 1751 -/- -/- -/- -/-

VEN 453 VEN 453 (ICA) mays OP Costeno Venezuela N/A N/A 9.02 N 63.72 W 146 -/- +/- -/- -/-

JAL 43 JAL 43 (CIMMYT) mays OP Tabloncillo Mexico N/A N/A 19.98 N 104.27 W 1330 -/- -/- -/- -/-

SIN 2 SIN 2 (INIFAP) mays OP Chapalote Mexico N/A N/A 24.80 N 107.42 W 61 -/- -/- -/- -/-

NAY 15 NAY 15 (INIFAP) mays OP Reventador Mexico N/A N/A 22.33 N 105.38 W 46 -/- -/- -/- -/-

PI217408 PI217408 (NCRPIS) mays OP Lonfellow

Flint USA N/A N/A 42.25 N 71.50 W N/A -/- -/- +/- -/-

OAX 70 OAX 70 (CIMMYT) mays OP Zapalote

Chico Mexico N/A N/A 16.33 N 95.23 W 46 -/- -/- -/- -/-

PI213793 PI213793 (NCRPIS) mays OP Assiniboine USA N/A N/A 46.82 N 100.82 W 457 -/- -/- -/- -/-

PI269743 PI269743 (NCRPIS) mays OP Shoe Peg USA N/A N/A 36.78 N 92.22 W 213 +/+ -/- -/- -/-

VEN 630 VEN 630 (NRC) mays OP Cacao Venezuela N/A N/A 8.60 N 71.17 W 201 -/- +/- -/- -/-

VEN 568 VEN 568 (NRC) mays OP Araguito Venezuela N/A N/A 8.90 N 64.22 W 183 -/- +/+ -/- -/-

OAX 68 OAX 68 (INIFAP) mays OP Bolita Mexico N/A N/A 17.47 N 97.30 W 1646 -/- -/- -/- -/-

RGS VII RGS VII (CIMMYT) mays OP Cravo

Riograndense

Brazil N/A N/A 30.00 S 53.00 W 366 -/- +/+ -/- -/-

BOV 635 BOV 635 (NRC) mays OP Cateto Bolivia N/A N/A 16.60 S 67.25 W 1801 -/- -/- -/- -/-

BOV 344 BOV 344 (ICA) mays OP Pisankalla Bolivia N/A N/A 21.52 S 64.75 W 1738 -/- -/- -/- -/-

BOV 587 BOV 587 (NRC) mays OP Pororo Bolivia N/A N/A 16.37 S 60.95 W 330 -/- -/- -/- -/-

SUR 797 SUR 797 (CIMMYT) mays OP Cateto

Nortista Suriname N/A N/A 5.75 N 55.22 W 61 +/- -/- -/- -/-

CUN 465 CUN 465 (NRC) mays OP Pollo Colombia N/A N/A 5.05 N 73.50 W 1600 -/- +/+ -/- -/-

AYA 32 AYA 32 (PCIM) mays OP Chullpi Peru N/A N/A 12.93 S 74.25 W 2350 -/- +/+ -/- -/-

ARG 461 ARG 461 (NRC) mays OP Capia

Amarillo Argentina N/A N/A 23.20 S 65.35 W 2287 -/- +/+ -/- -/-

URG II URG II (CIMMYT) mays OP Cateto Sulino

Uruguay N/A N/A 31.70 S 56.00 W N/A -/- -/- -/- -/-

CHI 349 CHI 349 (NCGRP) mays OP Cristalino

Norteno Chile N/A N/A 39.92 S 72.50 W 7 -/- -/- +/- -/-

MEX 6 MEX 6 (INIFAP) mays OP Palomero

Toluqueno Mexico N/A N/A 19.28 N 99.67 W 2652 -/- -/- -/- -/-

CHH 128 CHH 128 (INIFAP) mays OP Cristalino De

Chihuahua Mexico N/A N/A 29.22 N 108.13 W 2095 -/- -/- -/- -/-

CHH 158 CHH 158 (INIFAP) mays OP Azul Mexico N/A N/A 28.55 N 107.48 W 2040 -/- -/- -/- -/-

CHH 166 CHH 166 (INIFAP) mays OP Apachito Mexico N/A N/A 27.98 N 107.58 W 2400 -/- -/- -/- -/-

CHS 39 CHS 39 (INIFAP) mays OP Comiteco Mexico N/A N/A 15.23 N 92.27 W 1400 +/- -/- -/- -/-

GTO 69 GTO 69 (INIFAP) mays OP Celaya Mexico N/A N/A 20.48 N 100.97 W 1812 -/- -/- -/- -/-

JAL 753 JAL 753 (INIFAP) mays OP Serrano De

Jalisco Mexico N/A N/A 20.03 N 103.68 W 2520 -/- -/- -/- -/-

CHS 81 CHS 81 (INIFAP) mays OP Olotillo Mexico N/A N/A 16.60 N 92.72 W 686 +/- -/- -/- -/-

CHS 695 CHS 695 (INIFAP) mays OP Oloton Mexico N/A N/A 16.80 N 92.55 W 2390 +/- +/- -/- -/-

GRO 383 GRO 383 (INIFAP) mays OP Ancho Mexico N/A N/A 18.42 N 99.55 W 1200 -/- -/- -/- -/-

MEX 108 MEX 108 (INIFAP) mays OP Conico Mexico N/A N/A 19.10 N 99.58 W 2520 -/- -/- -/- -/-

MEX 212 MEX 212 (INIFAP) mays OP Cacahuacintl

e Mexico N/A N/A 19.30 N 99.65 W 2620 -/- -/- -/- -/-

PUE 510 PUE 510 (INIFAP) mays OP Elotes

Conicos Mexico N/A N/A 18.97 N 98.30 W 2200 -/- -/- -/- -/-

ZAC 180 ZAC 180 (INIFAP) mays OP Elotes

Occidentales Mexico N/A N/A 22.65 N 103.70 W 2050 -/- -/- -/- -/-

CHS 30 CHS 30 (CIMMYT) mays OP Vandeno Mexico N/A N/A 16.50 N 92.50 W 614 -/- -/- -/- -/-

GTO 191 GTO 191 (INIFAP) mays OP Zamorano

Amarillo Mexico N/A N/A 21.12 N 101.68 W 1870 -/- +/+ -/- -/-

VER 457 VER 457 (INIFAP) mays OP Coscomatepe

c Mexico N/A N/A 19.20 N 97.03 W 1800 -/- -/- -/- -/-

ZAC 182 ZAC 182 (INIFAP) mays OP Dulce De

Jalisco Mexico N/A N/A 22.65 N 103.70 W 2050 -/- -/- -/- -/-

MIC 328 MIC 328 (INIFAP) mays OP Mushito Mexico N/A N/A 19.68 N 102.12 W 2200 -/- -/- -/- -/-

SIN 34 SIN 34 (INIFAP) mays OP Dulcillo Del

Noroeste Mexico N/A N/A 25.07 N 107.50 W 500 -/- -/- -/- -/-

OAX 570 OAX 570 (INIFAP) mays OP Mixteco Mexico N/A N/A 17.42 N 97.48 W 2600 -/- -/- -/- -/-

SON 117 SON 117 (INIFAP) mays OP Blandito De

Sonora Mexico N/A N/A 28.97 N 109.40 W 410 -/- -/- -/- -/-

CHS 26 CHS 26 (INIFAP) mays OP Tepecintle Mexico N/A N/A 15.43 N 92.90 W 107 -/- -/- -/- -/-

NAY 191 NAY 191 (CIMMYT) mays OP Bofo Mexico N/A N/A 21.38 N 104.13 W 1000 -/- -/- -/- -/-

CHS 29 CHS 29 (CIMMYT) mays OP Tehua Mexico N/A N/A 15.75 N 92.72 W 1000 -/- +/- -/- -/-

CHS 521 CHS 521 (INIFAP) mays OP Zapalote

Grande Mexico N/A N/A 16.72 N 93.72 W 730 -/- -/- -/- -/-

CHS 650 CHS 650 (INIFAP) mays OP Motozinteco Mexico N/A N/A 15.37 N 92.25 W 1270 +/- -/- -/- -/-

CHH 150 CHH 150 (INIFAP) mays OP Palomero

Tipo Chihuahua

Mexico N/A N/A 29.35 N 107.75 W 2140 -/- -/- -/- -/-

CAM 48 CAM 48 (INIFAP) mays OP Nal-Tel Mexico N/A N/A 19.78 N 90.12 W 50 +/+ -/- -/- -/-

CHH 121 CHH 121 (INIFAP) mays OP Tuxpeno Norteno

Mexico N/A N/A 30.90 N 108.17 W 1470 -/- +/- -/- -/-

CHS 196 CHS 196 (INIFAP) mays OP Nal-Tel De

Altura Mexico N/A N/A 15.47 N 92.15 W 2300 +/- -/- -/- -/-

COA 21 COA 21 (INIFAP) mays OP Tuxpeno Norteno

Mexico N/A N/A 25.43 N 102.18 W 1400 +/- -/- -/- -/-

COA 25 COA 25 (INIFAP) mays OP Raton Mexico N/A N/A 25.75 N 102.97 W 1610 +/- -/- -/- -/-

GRO 157 GRO 157 (INIFAP) mays OP Conejo Mexico N/A N/A 17.12 N 100.47 W 30 -/- +/- -/- -/-

NAY 16 NAY 16 (CIMMYT) mays OP Tabloncillo

Perla Mexico N/A N/A 22.08 N 105.25 W 46 -/- -/- -/- -/-

NAY 24 NAY 24 (INIFAP) mays OP Harinoso De

Ocho Mexico N/A N/A 21.95 N 105.30 W 46 -/- -/- -/- -/-

NAY 185 NAY 185 (CIMMYT) mays OP Tablilla De

Ocho Mexico N/A N/A 21.68 N 104.07 W 1100 -/- -/- -/- -/-

OAX 565 OAX 565 (INIFAP) mays OP Serrano

Mixe Mexico N/A N/A 17.13 N 96.02 W 2350 -/- +/+ -/- -/-

OAX 566 OAX 566 (INIFAP) mays OP Mixeno Mexico N/A N/A 17.22 N 96.05 W 1850 -/- -/- -/- -/-

QOO 20 QOO 20 (INIFAP) mays OP Dzit Bacal Mexico N/A N/A 21.25 N 86.75 W 10 +/+ -/- -/- -/-

SIN 17 SIN 17 (INIFAP) mays OP Elotero De

Sinaloa Mexico N/A N/A 23.50 N 106.30 W 200 -/- -/- -/- -/-

SON 105 SON 105 (INIFAP) mays OP Onaveno Mexico N/A N/A 28.32 N 108.97 W 820 -/- -/- -/- -/-

VER 311 VER 311 (INIFAP) mays OP Arrocillo Amarillo

Mexico N/A N/A 19.80 N 97.25 W 2220 -/- +/+ -/- -/-

JAL 78 JAL 78 (INIFAP) mays OP Dulce De

Jalisco Mexico N/A N/A 21.18 N 102.88 W 1880 -/- -/- -/- -/-

B2 B2 (Goodman) mays OP Reid Yellow

Dent USA N/A N/A N/A N/A N/A -/- -/- +/+ -/-

VEN 604 VEN 604 (ICA) mays OP Canilla Venezuela N/A N/A 8.90 N 64.22 W 183 -/- -/- -/- N/A

COR 334 COR 334 (NRC) mays OP Cariaco Colombia N/A N/A 8.18 N 76.07 W 200 -/- +/- -/- -/-

ECU 458 ECU 458 (NRC) mays OP Chillo Ecuador N/A N/A 2.85 S 78.67 W 2195 -/- +/+ -/- -/-

ECU 964 ECU 964 (ICA) mays OP Chococeno Ecuador N/A N/A 0.67 S 79.50 W 46 -/- +/+ -/- -/-

PUN 4 PUN 4 (PCIM) mays OP Confite Puneno

Peru N/A N/A 15.93 S 69.88 W 3900 -/- +/+ -/- -/-

MAG 443 MAG 443 (NCGRP) mays OP Guirua Colombia N/A N/A 10.48 N 73.25 W 1860 +/- +/- -/- -/-

CUN 372 CUN 372 (ICA) mays OP Imbricado Colombia N/A N/A 4.95 N 74.38 W 2625 -/- +/+ -/- -/-

CUZ 66 CUZ 66 (PCIM) mays OP Kculli Peru N/A N/A 13.53 S 71.90 W 3268 -/- +/- -/- -/-

NAR 426 NAR 426 (ICA) mays OP Montana Colombia N/A N/A 1.18 N 77.25 W 2500 +/- +/- -/- -/-

BOY 462 BOY 462 (NRC) mays OP Pira Colombia N/A N/A 5.02 N 73.45 W 900 +/+ -/- -/- -/-

VEN 485 VEN 485 (ICA) mays OP Pira Venezuela N/A N/A 8.00 N 72.00 W 412 -/- -/- -/- -/-

APC 13 APC 13 (PCIM) mays OP Pisccorunto Peru N/A N/A 13.67 S 73.37 W 2900 -/- +/+ -/- -/-

SAN 329 SAN 329 (NRC) mays OP Sabanero Colombia N/A N/A 7.32 N 72.47 W 2626 +/+ -/- -/- N/A

YUC 148 YUC 148 (INIFAP) mays OP Nal-Tel Mexico N/A N/A 20.3 N 89.43 W 30 +/+ -/- -/- N/A

MOR 99 MOR 99 (INIFAP) mays Pepitilla Mexico N/A N/A 18.68 N 99.12 W 1040 -/- -/- -/- -/-

JAL 44 JAL 44 (CIMMYT) mays OP Jala Mexico N/A N/A 19.95 N 104.27 W 1300 -/- -/- -/- -/-

BOV 331 BOV 331 (ICA) mays OP Aysuma Bolivia N/A N/A 19.03 S 65.27 W 2127 -/- +/- -/- -/-

ARG 499 ARG 499 (CIMMYT) mays OP Capia Blanco Argentina N/A N/A 23.20 S 65.35 W 2287 -/- +/+ -/- -/-

SUR I SUR I (CIMMYT) mays OP Cateto Nortista

Precoce Suriname N/A N/A 5.75 N 55.22 W 61 -/- -/- -/- -/-

URG II URG II (CIMMYT) mays OP Cateto Sulino

Uruguay N/A N/A 31.70 S 56.00 W N/A -/- -/- -/- -/-

MEX 48 MEX 48 (INIFAP) mays OP Chalqueno Mexico N/A N/A 19.68 N 99.13 W 2256 -/- -/- -/- -/-

PUE 109 PUE 109 (INIFAP) mays OP Conico Mexico N/A N/A 18.23 N 97.57 W 2104 -/- -/- -/- -/-

BOV 992 BOV 992 (ICA) mays OP Coroico Bolivia N/A N/A 13.08 S 64.82 W 200 -/- +/+ -/- -/-

CUB 63 CUB 63 (CIMMYT) mays OP Cuban Flint Cuba N/A N/A 20.67 N 75.75 W 91 -/- -/- -/- -/-

SP III SP III (CIMMYT) mays OP Dente

Paulista Brazil N/A N/A 22.00 S 48.00 W 518 -/- +/+ -/- -/-

GUA 130 GUA 130 (CIMMYT) mays OP Dzit Bacal Guatemala N/A N/A 14.43 N 89.67 W 610 +/- +/- -/- -/-

CHI 421 CHI 421 (NRC) mays OP Harinoso

Tarapaqueno Chile N/A N/A 19.92 S 69.52 W N/A -/- +/- -/- -/-

BOV 961 BOV 961 (NRC) mays OP Karapampa Bolivia N/A N/A 21.83 S 64.13 W 1976 -/- +/- -/- -/-

BOV 567 BOV 567 (ICA) mays OP Morado Bolivia N/A N/A 16.47 S 67.47 W 2270 -/- +/+ -/- -/-

GUA 14 GUA 14 (INIFAP) mays OP Serrano Guatemala N/A N/A 15.28 N 91.43 W 1738 -/- +/- -/- -/-

TRN 10 TRN 10 (INIFAP) mays OP Tuson Trinidad N/A N/A 10.58 N 61.12 W 20 -/- -/- -/- -/-

PI214195 PI214195 (NCRPIS) mays OP Longfellow Canada N/A N/A 43.58 N 80.23 W 488 -/- -/- +/- -/-

JAL 127 JAL 127 (INIFAP) mays OP Mountain

Yellow Mexico N/A N/A 19.95 N 103.77 W 2060 -/- -/- -/- -/-

JAL 142 JAL 142 (INIFAP) mays OP Palomero De

Jalisco Mexico N/A N/A 20.03 N 103.68 W 2520 -/- -/- -/- -/-

Table S5. Primers used for ZmYAB2.1 genotyping and expression analyses

Primer Sequence (5’ – 3’) Description

CJ199 AGAGAAGGCGTTCGAGCATA

Marker for Spm insertion in W22 CJ198 CCACCATCAGTCACACACTT

CJ102 TGCATTTGCTTACGGTACTT

CJ096 TTCGAATGACTTGTAGTTCAG

Marker for Spm insertion in B73 CJ167 CCGAAAATGGTGTACAGTGC

CJ132 AGTGCCTTTGTTGACACTCG

CJ055 ACGTGCAGCATATGATCCAG Marker for 2bp/82bp insertions in Ki3 and Mo17

CJ085 TCAAGGTCCAAAATTTCAGC

CJ075 CTTATGTCCGGGTTGTTTGC qPCR primer pair for ZmYAB2.1

CJ085 TCAAGGTCCAAAATTTCAGC

wh182 CCAAGGCCAACAGAGAGAAA qPCR primer pair for GRMZM2G126010 (actin gene)

wh183 CCAAACGGAGAATAGCATGAG

CJ080 CTGGATGGCCCTCTGATTAG Primer pair for ZmYAB2.1 5’ UTR allele specific expression

CJ124 GACGACATTCCTTCGCCTTG

T385 TCATGGACAACGATGAGTGG qPCR primer pair for tb1

T388 CAATAACGCACACCAGGTCC

Table S6. PCR fragment sizes for alleles used in allele specific expression analysis

Allele PCR fragment size (bp)

A619 164

Oh43 164

MR12 150

MR19 153

TIL01 164

TIL11 160

Table S7. Allele specific expression results of ZmYAB2.1 with five or more reads from Lemmon et al. (2014)

F1 Hybrid Percentage of Reads Belonging to Maize (%) Total Number of Reads

B73 x TIL14 0 65

W22 x TIL14 0 15

Ki3 x TIL11 28 39

Ki3 x TIL03 10 31

References

Cook, J. P., M. D. McMullen, J. B. Holland, F. Tian, P. Bradbury et al., 2012 Genetic

architecture of maiuze kernel composition in the nested association mapping and inbred

association panels. Plant Physiol. 158: 824–834.

Darling, A. C. E., B. Mau, F. R. Blattner, and N. T. Perna, 2004 Mauve: multiple alignment of

conserved genomic sequence with rearrangements. Genome Res. 14: 1394–1403.

Lemmon, Z. H., R. Bukowski, Q. Sun, and J. F. Doebley, 2014 The role of cis regulatory

evolution in maize domestication. PLoS Genet. 10: e1004745.