Addgene – Helping Scientists Share Joanne Kamens, PhD Addgene, Executive Director

Addgene’s Mission

Accelerate research and discovery by improving access to useful research materials

and information

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Addgene Helps Scientists Share Plasmids

Nonprofit, mission-driven organization dedicated to facilitating collaboration and sharing in the scientific community

We promote this mission by making it easy to share plasmids!

Addgene: Then and Now

Founded in 2004 Ken Fan, Benjie Chen

and Melina Fan

Filling the Need Supports the Mission

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Plasmids Distributed by Addgene

Cumulative USCumulative Non-US

Currently, over 500 plasmids are distributed each day

Easier Collaboration Means Better Science

Requestor benefits • Quality control • Curation • Reproducibility improved • Plasmid selection/design support • Request from many labs at once • International access (>80 countries so far!) • Timely receipt of materials

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Requested Plasmids by Country

Distributor Partnerships for Global Reach

Our distributor in China solved shipping/customs issues here Customer support for shipping issues in a helpful time is zone available from BZY Please Email or call Addgene in the USA for technical science support

Growth of the Sharing Community

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Labs Depositing and Plasmids Deposited (*10/1/2015)

Deposited Plasmids by Country

Easier Collaboration Means Better Science

Depositor benefits • Quality control • Reproducibility improved • Save time on request fulfillment • Supported international sharing • Plasmids get more use • Protection against lab turnover • Archiving key reagents • Distribution statistics maintained • Increased exposure

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Depositing is Easy

Addgene PhD scientists will help during the entire process Easy online deposit process or contact Addgene for help entering data Can deposit before a paper is published so when the paper comes out, plasmid numbers are in the materials and methods Most new labs start by depositing their most requested plasmids (or plasmids they think should be getting more requests) Addgene takes care of the entire technology transfer process – scientists do not have to worry about this

Demonstrating Impact: Depositing in a Biological Resource Center Increases Citation Rates

“Climbing Atop the Shoulders of Giants: The Impact of Institutions on Cumulative Research.” Jeffrey Furman and Scott Stern. American Economic Review 101.5 (2011): 1933–1963.

Acknowledging our Depositors

Private PI Account Page • Detailed information on requests

(can also get email summaries) • Tech Transfer Officers see

distribution of all plasmids at their institution via TTO Acct page

Unique, PI public page

Resources and Curation

Addgene is Helpful

• 950 scientists who used Addgene’s customer service.

• Customer service inquiries: • 74% by email • 18% by telephone • 8% by web interface

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Please rate your experience with Addgene customer support

ExcellentGoodFairPoor

I think Addgene is fantastic. I will forward your details to PIs in the

Institute, that way potential plasmid donors will know you are around.

Depositor, University College Dublin

Scientists Support Collaboration

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Oh so, you’re not really a salesperson, you’re here to

make my life easier… Future depositor, CHOP

“I do science differently now that I can request

plasmids from Addgene”

Lab head at Yale

And Then Came CRISPR

The Addgene CRISPR/cas9 Plasmid Collection

>1,000 CRISPR/cas9 plasmids >100 depositing laboratories >50,000 requests fulfilled >15,000 requestors in 2,200 universities Note especially the CRISPR libraries—a reagent not usually made publicly available

Genome Engineering Distribution

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CRISPR Plasmids Still Popular

International Impact from Cambridge, MA

United States 48%

Japan 9%

China 7%

Germany 6%

Great Britain 5%

Canada 3%

France 2%

Korea (Rep of) 2%

Switzerland 2%

Australia 2%

Rest of World 14%

Distribution of Addgene's Top 20 CRISPR/cas9 Plasmids by Country

Rest of World = 50 Countries

CRISPR/cas9 Plasmid Depositors (so far)

Mario de Bono Mike Boxem Simon Bullock John Calarco Wenbiao Chen Qi-Jun Chen Yonglong Chen Lionel Christiaen George Church Jennifer Doudna Peter Duchek Benjamin Ebert Andrew Fire Klaus Foerstemann Hodaka Fujii Wataru Fujii Caixia Gao Charles Gersbach Bob Goldstein Manuel Goncalves

Melissa Harrison Izuho Hatada Akitsu Hotta Bo Huang Shenglin Huang Xingxu Huang Danwei Huangfu Masahito Ikawa Tyler Jacks Rudolf Jaenisch Keith Joung Sophien Kamoun Iskra Katic Jin-Soo Kim Masato Kinoshita Eric Lander Ji-Long Liu David Liu Sebastian Lourido Timothy Lu Rene Maehr

Luciano Marraffini Craig Mello Eric Mendenhall Kiran Musunuru Richard Myers Kate O'Connor-Giles Wayne Parrott Lei Stanley Qi David Root David Sabatini Moritoshi Sato Alex Schier Frank Schnorrer Jen Sheen David Sibley William Simonds Erik Sontheimer Paul Sternberg Robert Stupar James Thomson

Ron Vale Jordan Ward Wensheng Wei Jonathan Weissman Susan Wente Jill Wildonger Scot Wolfe Takashi Yamamoto Yinong Yang Kosuke Yusa Donald Zack Mikel Zaratiegui Feng Zhang Yunde Zhao Huimin Zhao YOUR NAME HERE

Rapid Impact in Genome Engineering

Figure from the Genome Biology special Genome Engineering edition, in press

Educational Resources

The Community Shares CRISPR 101: Protocols, Tips, and Advice

CRISPR Software Matchmaker: Choosing the Best CRISPR Software Cpf1: A New Tool for CRISPR Genome Editing CRISPR 101: Mammalian Expression Systems and Delivery Methods Genome-wide Screening Using CRISPR/Cas9 Mapping the 4D nucleome with CRISPR/Cas9 CRISPR 101: Validating Your Genome Edit Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall & Myers A match made in heaven: CRISPR/Cas9 and AAV Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards CRISPR 101: Non-Homologous End Joining CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video] All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering etc., etc.

blog.addgene.org

Collaboration Speeds Discovery “The number of scientific papers with CRISPR in their title rose from 90 in 2012 to 741 (and counting) this year. That was partly Zhang’s doing: he has been using a non-profit called Addgene to distribute genetic and other material, called reagents, to biologists around the world.”

-STAT News

“‘The rapid adoption of the Cas9 technology was also greatly accelerated through a combination of open-source distributors such as Addgene.’’

-Patrick Hsu, Eric Lander, Feng Zhang Cell 2014

Thanks for listening. Questions? Please talk with me between

sessions if you have feedback or questions – Addgene wants to hear how we can help make things easier for you.

Twitter: @addgene

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blog.addgene.org

help@addgene.org

617-225-9000