ain shams university/ faculty of medicine medical biochemistry & molecular biology department...

Ain Shams University/ Faculty of Medicine

Medical Biochemistry & Molecular Biology DepartmentSafety Rules for Medical Biochemistry Students

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BY ALL STAFF MEMBERS

OF MEDICAL BIOCHEMISTRY DEPARTMENT


Medical Biochemistry & Molecular Biology Department

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Welcome To The Biochemistry Lab

Always remember every time:To Be on timeTo record your attendance by your IDTo Get Your White CoatTo Get Your Student recordTo be creative ! To follow your contract items



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Principle

Remember that the lab is a place for serious work! Careless behavior may endanger yourself and others and will not be tolerated!

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ILO:By the end of this topic, the student will be able to: Practice basics of safety procedures and apply the different general rules of safety during learning in Lab.

Purpose

Provide guidance and protocols for the protection of students from laboratory hazardous materials.

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Why does it matter?• Safe working protects:

– You– Other lab workers– Cleaners– Visitors– Your work

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Appearance and attitude in the lab.General work procedure.Hygiene practice.Emergency procedure.

Safety Rules for Medical Biochemistry Students includes :

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Appearance and attitude in the lab

While working in the lab, the following instructions should be followed:

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You must wear your clean white coatاالبيض البالطو ارتداء الخاص يجب

بك

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http://www.crestock.com/images/3770000-3779999/3779848-xs.jpg

http://www.crestock.com/images/3770000-3779999/3779848-xs.jpg



Do not use any flammable materials

such as perfumes.المواد تستعمل ال

مثل لالشتعال القابلهالبرفانات

Tie up long hair during laboratory activity

الشعر ترك عدم يجبفى العمل اثناء منسدال

المعمل

You must insert the jewelry and veil into the coat

االكسسوار ادخال يجبالبالطو داخل فى اوالحجاب

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Try always to wear leather shoes that cover your feet.

حاول دائما ارتداء حذاء جلدى يغطي القدمين

• Sandals and open-toed shoes do not protect your feet from broken glass that is frequently found in the lab

• Also, leather shoes protect your feet from chemical spills – canvas shoes do no

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Place books and personal items in the cabinet

والمتعلقات الكتب ادخال يجبالشخصية

المخصص المكان فى

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Only laboratory manuals are permitted into the working area

بم فقط العملى ذيسمح كراتالعمل منطقة فى والكشكول

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Listen to your instructor carefully and stand in your proper position in the lab.مشرفك لتعليمات استمع

مكانك في دائما وقف بحرصالمخصص

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Eating and drinking are not allowed in the lab.

فى به مسموح غير الشرب او االكلالمعمل

Put all food and drinks in your bag.



Do not sit on lab benches.

أثاث على تجلس ال المعمل

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side talks & the mobile are not allowed in the lab.

المعمل في زمالئك مع تتحدث ال. المحمول في او

In a laboratory setting no pushing; no shoving.

Avoid Horseplay



At the end of the lab period: Exit the lab in

an orderly manner.Again: no running, no pushing, no shoving.

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Read the instruction guide ahead of performing the experiment.

التجربة اجراء قبل التعليمات دليل .اقرأ

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General work procedure

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When in doubt – ASK!!!عند الشك----اســــــــأ ل

• Do not carry out a new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working

• DO NOT GUESS!!!!



You should wear approved safety goggles or safety glasses when

instructed in the lab. بذلك كلفت اذا بالمعمل الوقاية نظارات ارتداء يجب

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Personal Protective Equipment



This is not acceptable!!!

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Wear gloves if you will come in contact with blood specimens من عندما الدم اعينة القفاز رتد

مالمسة For even more dangerous liquids, thick gloves should be used.

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Use only clean & dry glassware and

never use broken or chipped glassware.

فقط استعملنظيفه زجاجيات

وسليمه جافه

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Check glassware for stars or cracks.

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Use only materials and equipments authorized by the instructor

ال اجهزة او مواد اى استعمالالمسئول المشرف باذن اال يكون

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Be aware if the chemicals used are hazardousان الخطيرة الكيماويات مع حذرا كن

استعملت

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Pour from large containers to smaller ones.

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Always ADD ACID to water Not the reverse !!

Do not spit into acid!” a good phrase to remember this rule.

acid

water



Use the flame with extreme caution and

Keep your head and clothing away from the flame

بحرص اللهب استعملشديد

ومالبسك راسك واجعلاللهب عن بعيدا

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Never carry flammable or hot and dangerous equipment or chemicals

through the lab.باالدوات ابدا تتحرك ال

الضارة و الساخنة اوالكيماوياتالمعمل داخل لالشتعال اوالقابلة

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Handle hot glassware with holder or tongs.

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Always point test tubes away from yourself and others when

heatingبعيدا االختبار انابيب دائما وجه

اثناء غيرك وعن نفسك عنالتسخين

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Never taste anything or touch chemicals with hand

الكيماويات ابدا تتذوق الباليد تالمسها او

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Test for odor of chemicals only by waving your hand above the container & sniffing cautiously from a distance

طريق عن فقط يكون للكيماويات الرائحة اختبارالمصدر اعلى من اليد تحريك

بعد على من بحذر االستشناق مع

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Never pour used reagents back into the bottle

مرة المستعملة الكيماويات التردمصدرها الى اخرى

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Keep lids on bottles and containers when not in use.

عدم حاله في الزجاجيات بتغطيه قماستخدامها

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• Use a rubber suction bulb or other device to fill a pipette should be used whenever available

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Safety Rules

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Inform the instructor immediately about any non properly working

equipment or any difficulty

مشرف ابالغ عليكحدوث عند المعمل

اي جهاز اي في عطلصعوبة اي وجود

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Hygiene practice

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Keep hands away from face, eyes and clothes during lab

من ألى اليد مالمسة عدمالمالبس و والعينين الوجه

العملى اثناء

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Keep tabletops clean. Return all equipment to its original location before leaving the lab. Clean all

spills immediately.

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Always clean the instruments & laboratory area before leavingالمعمل مغادره قبل عملك ومنطقة أدواتك نظف دائما



Broken Glass

Sweep it up right away.

Always clean the instrumentsأدواتك نظف دائما



Know how to dispose of waste

Dispose all waste materials according to your instructor order.

? ? ? ?

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Wash hands with soap and water before leaving the lab.

قبل والصابون بالماء يديك اغسلالمعمل مغادره

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Emergency procedure

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Emergency procedures includes:1-First aid procedures are available. First aid form and safety materials are included (Appendix 1).2- Inform the supervisor immediately about any problem or accident in the lab. An accident report form is available in the main lab office (Appendix 2).3- If any accident happened, as burn from an acid or alkali, wash the affected area immediately with plenty of running water.



In case of accident as an acid or alkali burns

Wash the affected area immediately with plenty of

running waterمن كحرق حادث اى وقع لو

قلوى او حمضفى المصابة المنطقة اغسل

الجارى الماء من بكثير الحال

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Inform the supervisor immediately about any

problem or accident in the lab

عند فورا المشرف ابلغاو مشكلة اى حدوث

بالمعمل حادث

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PLATFORM

PLATFORM

Preparation room

Preparation room

EXITEXITEXITEXIT

Lab benches

IReport RoomIReport RoomChemical &Reagents Area

Lab Safety & First Aid

Biochemistry and Molecular biology Department Lab Diagram

Instructor

PLATFORM

PLATFORM



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Know the locations and operating

instructions for Safety equipments

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Fire extinguishers

Fire blanket

first aid kit

eye washes

http://en.wikipedia.org/wiki/File:FireExtinguisherABC.jpg

http://www.cpr-savers.com/principal/firstaidkits.html



First AidInjury: BurnsWhat To Do: Immediately flush with

cold water until burning sensation is lessened.

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First AidFainting:

To Do: Provide fresh air and keep the head lower than the rest of the body.

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First AidEye Injury: EyesWhat To Do: Flush eyes

immediately with plenty of water for at least 15 minutes. If a foreign object is lodged in the eye, do not allow the eye to be rubbed.

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First Aid

Skin Injury: Spills on the skinWhat To Do:

Wash with large quantities of water. For acid spills, apply baking soda

solution. For base spills, apply vinegar or boric acid.

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Thank you

Have a Safe Year!

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