8/8/2015 slide 1 oral presentation for chem 201: title of lab goes here u.r. name lab partner: t.h....
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04/19/23 Slide 1
Oral Presentation for Chem 201:
Title of Lab Goes Here
U.R. Name
Lab Partner: T.H. Guy
Presentation date: 04/19/23
04/19/23 Slide 2
Introduction
The INTRODUCTION introduces the talk Include in your Introduction
The problem you addressed Your experimental approach to the problem The significance of your work
Other ground rules: Bullet points are best
Bullet points should be just that Say full sentences; don't write them
Don't use too many colors Don't put too much material on one "slide"
Use more slides than less slides Make sure that everything you present is legible. Use 14-point type or bigger
Leave white space as a border around every slide!
04/19/23 Slide 3
Details of the Experiment
Because you can only put one topic/thought per slide, you will need several slides to fill in critical details.
The slides that come after the Introduction will be the equivalent of the Experimental section Provide any theory required that is critical to the calculations and
results you will obtain Provide an overview of the instrumentation used and any details
about instrumentation that are critical to the data you obtained Describe the experiments you have performed Present the critical equations for your calculations of data Present any other critical details
04/19/23 Slide 4
Details – Cont.
State logically (in stepwise fashion) the experimental details and what you did
Give big strokes—not tedious details. Examples: Prepared solutions of dimuglioglugtane (DMGG) at concentrations of
0.01, 0.1, and 1 M in CDCl3. Acquired 1H-NMR spectra
If the number of spectra or other acquisition parameters are important, include as sub-points.
Tell how you transformed the raw data into meaningful results Make sure you keep everyone focused on the purpose of the
experiment Discuss the importance of your findings after you present your
results.
04/19/23 Slide 5
Other Points
Cite sources of information in your presentation. Including: Articles used in your "overview" etc. All figures that you present that are not your own Sources of information
Citations are best placed at the bottom of the slide
Pictures are always better than words Present the pictures; say the words Data tables:
Avoid where possible. Use bar graphs, etc. instead of data tables Keep the tables simple and small
Here is a good place to put any citations you need on any page.
04/19/23 Slide 6
Example of Introduction Using Both Graphical Info and Bullet Points:
Sulfur Amino Acids (SAA)
Methionine An essential amino acid
Cysteine Nonessential amino acid Synthesis dependent methionine
Homocysteine Produced from methionine metabolism Contributes S for cysteine synthesis
Elevated homocysteine levels correlate with cardiovascular disease risk
Methionine (Met)
NH2
O
HOS
CH3
Cysteine (Cys)
NH2
O
HO SH
Homocysteine(Hcy)
NH2
O
HOSH
04/19/23 Slide 7
Example of an Experimental Procedure in Graphical Terms:
Method to Reduce and ModifyProtein-bound Homocysteine for Measurement by GCMS
From MacCoss et al., Anal. Chem. 1999
NH2
OH
O
SS
N
NSH
O
O
H3C
H3C
SH
NH2
OH
O
SH N
N S
O
O
H3C
H3C
S
N
NH2
OH
O
S
NNH
OtBDMS
O
S
tBDMS
Homocysteine Bound to Protein via a Disulfide Bridge
+
MTBSTFA
S-4-ethyl pyridine bis-tBDMS homocysteine
1
2
3
Homocysteine
DMH
N
4-vinylpyridine
04/19/23 Slide 8
Graphical Example of Results:
EI & NCI Mass Spectra of Leucine as the HFBPr Derivative
60 80 100 120 140 160 180 200 220 240 260 280 300 3200
1000
2000
3000
4000
5000
6000
7000
Abundance
55
69
114 131169
214
240
253
266
282
313
m/z
[M-COOPr]+
[M-C4H8]+?
CF3+
HFB-NH2+ ?
EI
160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 4600
5000
10000
15000
20000
25000
Abundance
160
178
198 243281
311
349
368 452
m/z
[M-H]-
[M-HF]-
?NCI
HFB = heptafluorobutyrylPr = n-propyl ester
CHNO
O
C3H7
C3F7O
[M-COOPr]+
MW = 369
04/19/23 Slide 9
Presenting Results & Discussion
Don't give ALL the data and ALL the results you have obtained Determine what you believe are the key results, and present
them Organize your talk around the Discussion
Figure out what results are required to support the points you want to discuss. Those are the results that you need to present.
Some data may need to be presented just to confirm that you correctly collected the results that you think you collected Examples: Data that demonstrate the instrumentation being used is working Data that demonstrate the experimental conditions are being met
04/19/23 Slide 10
Graphical Example of an Almost Too Busy Table: Amino Acid Composition of Select Protein Sources
Amino AcidHen Egg Muscle Liver
mole %
Alanine Ala 9.2 9.5 9.9
Arginine Arg 4.8 5.0 4.3
Aspartate + Asparagine Asx 6.6 7.9 7.9
Cysteine Cys 2.4 1.5 1.8
Glutamate + Glutamine Glx 10.2 13.0 10.5
Glycine Gly 5.9 8.7 8.0
Histidine His 2.0 2.4 2.2
Isoleucine Ile 6.4 4.7 5.0
Leucine Leu 8.4 8.0 9.1
Lysine Lys 5.5 7.6 6.7
Amino AcidHen Egg Muscle Liver
mole %
Methionine Met 2.6 2.2 2.3
Phenylalanine Phe 3.3 3.6 4.1
Proline Pro 4.8 5.7 5.7
Serine Ser 10.6 6.3 6.7
Threonine Thr 5.3 0.5 5.2
Tryptophan Trp 1.0 0.7 1.0
Tyrosine Tyr 3.0 2.2 2.6
Valine Val 8.0 6.1 6.8
04/19/23 Slide 11
Graphical Example of the Table Data as an Almost Too Busy Bargraph: Amino Acid Composition of Select Protein Sources
0%
2%
4%
6%
8%
10%
12%
14%
Am
ino
Ac
id C
on
ten
t(m
ole
fra
cti
on
)
Hen EggMuscleLiver
highin
egg
eachare
double
lowin
egg
not very abundant
04/19/23 Slide 12
Example of an Annotated, Scanned Figure: ESI-MS Spectrum of Horse Apomyoglobin
ApomyoglobinMW=16,951.2
+18
+20
+16
+14
+12
+10+22
m/z
04/19/23 Slide 13
Conclusions
You must have one or more Conclusions slides
The Conclusions Reiterate the key points of what you have accomplished State (for the last time) what is important about what you have done
04/19/23 Slide 14
Additional Constraints
At the time of your oral presentation, you need to:
1. Distribute a 1-page abstract of your talk to the class, TA's and instructor
2. Distribute copies of the slides that you will use to your TA's & instructor Printed no more than 2 per page
3. Give an annotated appendix of all figures, tables, data, etc. to your TA This appendix is the same as you would prepare for a regular
written laboratory.
04/19/23 Slide 15
Final Points
Plan on speaking for 30 (25-35) min with 10 min at the end for questions
Practice your talk before you actually give it! Be on time and be ready to go
Technical problems will cost you valuable speaking time
You can use for your presentation Slides displayed from a computer projection device
Angell B203 has a PC w/ USB port (not a Mac) Overheads Blackboard (as supplemental)
You get up to 20 points (your presentation is worth 50 points) for participating in asking questions and discussing the presentations
If you not ask questions or contribute to the discussion of the talks, you don’t get any points
Generally one question per talk for 4-5 talks will get you the 20 points
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