a biology and engineering cooperative project james w. stevens m. karen newell university of...

A Biology and A Biology and Engineering Engineering

Cooperative ProjectCooperative ProjectJames W. StevensJames W. StevensM. Karen NewellM. Karen Newell

University of Colorado at Colorado University of Colorado at Colorado SpringsSprings

30 April 200530 April 2005

general backgroundgeneral background

connection between cellular connection between cellular metabolic pathways and immune metabolic pathways and immune system responsesystem response

surface signals tied to mitochondrial surface signals tied to mitochondrial activity and fuelactivity and fuel

cooperative effortcooperative effort

biologybiology– science driverscience driver– cell culturescell cultures

growinggrowing conditionsconditions

– cell counting and cell counting and physical physical preparationpreparation

engineeringengineering– data acquisitiondata acquisition– temperature temperature

measurementsmeasurements– data reductiondata reduction– data smoothingdata smoothing

background-this projectbackground-this project

2 mouse leukemia lines2 mouse leukemia linesL1210 – not drug resistantL1210 – not drug resistant

L1210/ddp– drug resistantL1210/ddp– drug resistant cultured to 10^6 to 10^8 cellscultured to 10^6 to 10^8 cells stressed with bleachstressed with bleach

– measurable, repeatable differencesmeasurable, repeatable differences– different culture conditions, metabolic different culture conditions, metabolic

statesstates

cumulative heat release

-4

-2

0

2

4

6

8

10

12

14

16:51:36 16:52:19 16:53:02 16:53:46 16:54:29 16:55:12 16:55:55

time

jou

les

L1210ddp L1210

dt

dEWQG

)( TThAdt

dTmcG

data analysisdata analysis

first law of thermodynamics:first law of thermodynamics:

measured heat generation rate

-2

-1

0

1

2

3

4

5

6

16:52:05 16:52:10 16:52:14 16:52:18 16:52:23 16:52:27 16:52:31

time

wat

ts

L1210ddp L1210

0 0.2 0.4 0.6 0.8 10

0.5

1

*

*

*)(dt

bt

ec

aety

data smoothingdata smoothing

least square curve fit:least square curve fit:

minimize minimize

2ˆ*)( iyty

measured heat generation rate

-2

-1

0

1

2

3

4

5

6

16:52:05 16:52:10 16:52:14 16:52:18 16:52:23 16:52:27 16:52:31

time

wat

ts

L1210ddp, smoothed L1210, smoothed L1210ddp L1210

summarysummary

leverage complementary capacities– leverage complementary capacities– specific to people and projectspecific to people and project

involve studentsinvolve students

plunge into a new areaplunge into a new area

other cooperative efforts at UCCSother cooperative efforts at UCCS

biomechanics –biology, mechanical biomechanics –biology, mechanical engineeringengineering

orthopedic mechanics –MERIC, orthopedic mechanics –MERIC, biology, mechanical engineeringbiology, mechanical engineering

feedback controls –MAE, biologyfeedback controls –MAE, biology microgravity – MAEmicrogravity – MAE assistive technologies – MATH, assistive technologies – MATH,

biologybiology